Food Bioscience
·
2026
·
IF 5.9
Liuyi Wei,Taoying Wu,Hao Wu,Li Wen,Bo Cui,Yunhui Cheng
Rice-derived peptides are a sustainable protein source with emerging lipid regulation functions. In this study, peptides with novel pancreatic lipase (PL) and cholesterol esterase (CE) inhibition from rice protein hydrolysates (RPHs) were identified by peptidomics. Then their alleviating effects and mechanisms on lipid accumulation in cells were investigated. 55 peptides were identified, showing significant enrichment of Phe, Pro, Arg, and Leu, with hydrophobic/polar amino acids at terminal positions. Among them, five peptides (WQ7, FR6, GF6, GA7, and GY7) exhibited potent inhibitory activity against PL and CE, with IC 50 values of 0.19-0.70 μg/mL and 0.22-0.25 μg/mL, respectively. Molecular docking studies revealed that five peptides bind stably to the active sites of PL (Phe77, Tyr114, Phe215, and His263) and CE (Ser194 and His435) through hydrogen bonding, PI-alkyl, and PI-PI interactions. Dynamic simulations demonstrated that three peptides (WQ7, FR6, and GF6) form stable complexes with both CE and PL, exhibiting strong binding affinity. In vitro study confirmed that these peptides reduced levels of TC, TG, and LDL-c while increasing HDL-c content, with WQ7 showing superior efficacy. Western blot and qPCR results indicated WQ7 regulated lipid metabolism by suppressing the PPARγ pathway and downregulating the protein expression of related factors, such as ACS, FASN, and SREBP-1c. This study showed that the WQ7, a multifunctional bioactive peptide, demonstrates direct enzyme-inhibitory activity and regulates core transcriptional pathways, providing theoretical support for utilizing rice protein in the development of lipid-lowering functional food.
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高影响因子
MOLECULES
·
2023
·Vol. 28
·期 6
·2611
·
IF 4.6
Hui Ye,Yuqin Luo,Yanjie Li,Xiangyun Wang,Hongmei He,Jinhua Jiang,Jianzhong Yu,Changpeng Zhang
A simple and sensitive method for the simultaneous quantitation of prohexadione-Ca and uniconazole in the field experiment ofOryza sativaL. andCitrus reticulataBlanco was established using solid-phase extraction (SPE) with polymer anion exchange (PAX) and Florisil followed by LC-MS/MS. The method demonstrated excellent linearity (R2> 0.999 0), trueness (recoveries between 95~105%), precision (CVs between 0.8~12%), sensitivity, and repeatability (LOQ of 0.05 and 0.01 mg/kg, respectively). Residue tests were conducted in the field at 12 representative sites in China, revealing final concentrations of prohexadione-Ca and uniconazole in brown rice, rice hull, and rice straw to be below 0.05 mg/kg, while in whole citrus fruit and citrus pulp, they were below 0.01 mg/kg. These were below the maximum residue limits specified in China. The chronic dietary risks of prohexadione-Ca and uniconazole in rice crops and citrus fruits were calculated to be 0.48% and 0.91%, respectively. Our research suggests that the chronic risk associated with the daily consumption of rice crops and citrus fruit at the recommended dosage is acceptable.Keywords:Oryza sativaL.;citrus fruit;dietary intake risk assessment;plant growth regulators
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高影响因子
LAB ON A CHIP
·
2019
·Vol. 19
·期 14
·2383-2393
·
IF 6.914
Hui Hui Chai,Feng Chen,Shu Jie Zhang,Ya Dan Li,Zhi Song Lu,Yue Jun Kang,Ling Yu
The root system of plants is a major component of their bodies in terms of both function and bulk. The investigation of root system development is greatly assisted by microfluidic devices, which improve the spatial and temporal resolution of observations without destroying tissue. In the present study, a multi-chamber petaloid root-growth chip was developed for studying the development and physiology of root systems that have thin branching structures (i.e., fibrous root systems). The petaloid root-growth chip includes a central seed germination chamber and five root-growth chambers for observing the development of fibrous roots. The proposed device was applied for investigating the root system development of Oryza sativa. The phenotype and growth kinetics of O. sativa root systems grown in the proposed device were compared with those obtained during growth in a conventional conical flask with agar-based medium, and the results indicated that cultivation in the miniaturized device did not delay root system growth in the early stage (≤2 weeks). In addition, the transparent device enabled the non-destructive observation of the developmental and microstructural characteristics of the roots, such as the root caps, root border cells, and root hairs. Moreover, the ability to control the microenvironment in each of the five root-growth chambers individually facilitated the investigation of specific adaptations in the fibrous root growth of single O. sativa seedlings to different drought stresses. Accordingly, five polyethylene glycol (PEG)6000-induced drought stress conditions were established in the five root-growth chambers to investigate the root development of a single O. sativa seedling in the central germination chamber. In situ observations demonstrated that the different PEG6000-induced conditions affected the root growth responses and root microstructural adaptations of the single seedlings in each root-growth chamber. Therefore, the petaloid root-growth microfluidic chip can eliminate the effects of variations in different plant seeds to reveal the responses of plants to different environmental conditions more objectively while concurrently allowing for non-destructive observations at very high spatial and temporal resolution.
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Journal of Cleaner Production
·
2025
·
IF 10
Zhiheng Wang,Jingtao Hou,Khurram Shehzad,Shuanglian Xiong,Menghua Cao,Shuxin Tu
Organosilicon exhibits exceptional potential for reducing cadmium (Cd) uptake and accumulation in crops, owing to its molecular structure-endowed high membrane permeability and biocompatibility. However, the current understanding of the mechanisms by which organosilicon mitigates Cd toxicity in rice remains fragmented. We combined controlled hydroponic experiments and foliar organosilicon-sprayed field trials, integrating physiological, biochemical, transcriptomic, histochemical, and in vitro simulation analyses to clarify the underlying mechanisms. Results demonstrated that organosilicon reduces Cd uptake and accumulation and mitigates its toxicity through a synergistic triple mechanism: by reinforcing the root apoplastic barrier via enhanced lignification and suberization, by promoting vacuolar Cd compartmentalization in leaves, and by activating the plant defense system via potentiated glutathione-dependent antioxidant and glyoxalase pathways. Field trials revealed that foliar organosilicon application reduced Cd concentration in rice grains by 48.89 %–52.87 %, decreased Cd-associated health risks by 56.76 %–57.87 %, increased rice yield by 9.26 %–9.98 %, and enhanced grain nutritional quality. This study is the first to unveil the synergistic triple mechanism through which organosilicon mitigates rice Cd toxicity and accumulation. Given its low cost and high efficiency, this approach provides a practical and cost-effective strategy for using silicon-based materials in heavy metal-contaminated agricultural lands.
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高影响因子
Journal of Food Measurement and Characterization
·
2021
·Vol. 15
·期 2
·2064-2077
·
IF 2.431
Zhang Dong,Duan Xiaoliang,Wang Yuanyuan,Shang Bo,Liu Hui,Sun Hui,Wang Yuehua
The main aim of this study is to evaluate and compare the physicochemical properties, chemical composition, and in vitro antioxidant activities of rice bran oil (RBO) from five most popular japonica rice ( Oryza sativa L.) varieties planted in China. It was found that the AV and PV of RBOs were 0.45–1.18 mg KOH/g and 0.72–1.73 mmol/kg, individually. The L *, a *, and b * values of RBOs were 24.18–26.70, 1.84–2.54, and 6.35–9.01, respectively. RBOs were rich in linoleic acid (38.63–43.50%), oleic acid (34.23–41.20%), and palmitic acid (16.26–18.93%). POL + SLL (17.44–19.29%), OLL (16.06–17.67%), OOL (13.79–17.79%), and PLL (11.70–14.79%) were the predominant triacylglycerols of RBOs. RBO from different rice varieties contained abundant bioactive constituents such as tocopherols and tocotrienols (948.59–1461.90 mg/kg), squalene (2055.65–4456.79 mg/kg), γ-oryzanol (19391.15–30024.09 mg/kg), phytosterols (10632.74–13948.17 mg/kg), and polyphenols (127.55–358.84 mg/kg). Pigments such as carotenoids and chlorophylls varied from 6.47 to 14.29 mg/kg and 0.79 to 12.96 mg/kg. The DPPH, ABTS, FRAP, and ORAC value were 837.41–1055.64, 1592.38–2106.47, 244.27–557.13, and 170.16–1776.41 µmol TE/100 g, respectively. This work could provide useful information to consumers for choosing suitable vegetable oils based on their health needs.
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高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2023
·
IF 13.6
Luoqin Shen,Ping Zhang,Yanyao Lin,Xinting Huang,Siyi Zhang,Zhiheng Li,Zhiguo Fang,Yuezhong Wen,Huijun Liu
Antibiotics and microplastics (MPs) are two emerging pollutants in agroecosystems, however the effects of co-exposure to antibiotics and MPs remain unclear. The toxicity of florfenicol (FF) and polystyrene microplastics (PS-MPs) on rice seedlings was investigated. FF and PS-MPs caused colloidal agglomeration, which changed the environmental behavior of FF. FF inhibited rice growth and altered antioxidant enzyme (superoxide dismutase, peroxidase, and catalase) activities, leading to membrane lipid peroxidation; impaired photosynthetic systems, decreased photosynthetic pigments (Chlorophyll a, Chlorophyll b, and carotene), chlorophyll precursors (Proto IX, Mg-Proto IX, and Pchlide), photosynthetic and respiratory rates. The key photosynthesis related genes ( Psa A, Psa B, Psb A, Psb B, Psb C, and Psb D) were significantly down-regulated. The ultrastructure of mesophyll cells was destroyed with chloroplast swelling, membrane surface blurring, irregular thylakoid lamellar structure, and number of peroxisomes increased. PS-MPs mitigated FF toxicity, and the IBR index values showed that 10 mg∙L −1 PS-MPs were more effective. Metabolomic analysis revealed that the abundance of metabolites and metabolic pathways were altered by FF, was greater than the combined "MPs-FF" contamination. The metabolism of amino acids , sugars, and organic acids were severely interfered. Among these, 15 metabolic pathways were significantly altered, with the most significant effects on phenylalanine metabolism and the citric acid cycle ( p < 0.05).
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PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Xunzhi Deng,Qinyao Li,Jing Lu,Yilin Liu,Haiou Li,Ze Liao,Gang Huang,Langtao Xiao,Zhoufei Luo
Strigolactones (SLs) are novel plant hormones that play crucial roles in regulating plant growth and development. The stereoisomers of SLs often exhibit distinct biological activities, making their chiral separation and analysis essential for understanding their regulatory roles in plant branching. This study focused on the chiral separation and characterization of SL stereoisomers, including GR24 (synthetic) and strigol (natural). A magnetic nanocomposite (Ni₃(HITP)₂@Fe₃O₄@rGO) was synthesized for SL extraction, combined with SFC-MS/MS for precise separation and quantification. The method exhibited excellent linearity (0.2–50 ng/mL), minimal matrix effects (1.64–2.17 %), and low detection limits (1.93–3.76 pg/mL). Recoveries ranged from 86.90 % to 106.37 % with RSDs of 2.56 %–13.54 %. Under nitrogen and phosphorus stress, only (+)-strigol was detected in the roots of Oryza sativa L., suggesting its regulatory role under stress conditions. This reliable method provides robust support for exploring the physiological roles of SL stereoisomers in plants, shedding light on their functions in stress response and development.
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高影响因子
CEREAL CHEMISTRY
·
2024
·Vol. 101
·期 5
·1106-1117
·
IF 2.2
Yi Zhou,Xin Wu,Jinxin Shen,Daijun Yang,Qun Lu,Changjiang Long,Rui Liu
Background Black rice is rich in various nutrients, such as anthocyanins, which may undergo some changes during cooking. However, previous studies have been mainly focused on a single substance and paid less attention to the changes in overall nutrient components. Here, we performed widely targeted metabolomics analysis on the chemical compositional changes in a new black rice variety (‘Huamoxiang No.3’) under three cooking modes, including bottom heating (BH), induction heating (IH), and IH + pressure. Results Among the detected 1458 metabolites, 276 and 108 metabolites were differential metabolites after cooking and in three cooking modes, respectively. Cooking increased the contents of most peptides, phenolic acids, and organic acid compounds but significantly decreased those of lipids and flavonoids, particularly anthocyanins. However, phenolic acids such as chlorogenic acid, erucic acid, and p -coumaric acid increased significantly, possibly due to the release of bound phenols and degradation of flavonoids to phenolic acids, such as thermal degradation of C3G to protocatechuic acid and among the three heating modes, the IH + pressure mode resulted in the most significant changes. Conclusion Although cooking reduced the content of flavonoids and anthocyanins, the increase in phenolic acids and other substances contributed to the healthy effect of cooked black rice, and the content of these active substances increased more significantly in the IH + pressure mode.
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高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Yanni Wu,Shudong He,Tiange Pan,Xinya Miao,Jie Xiang,Yongkang Ye,Xiaodong Cao,Hanju Sun
In order to fortify γ-aminobutyric acid (GABA) of brown glutinous rice (BGR), pre-germination strategy was employed, and effects of low-frequency (28 kHz) ultrasound treatment combined with CaCl 2 stress on the sprout length, germination rate, morphology, color, water, total polyphenol content (TPC), starch, protein, GABA contents and relevant metabolites were investigated. The germination rate would be inhibited under CaCl 2 concentration ≥ 2.0 % during 24 h soaking without ultrasound treatment, and no significant difference was also observed combined with 9 h ultrasound treatment. Ultrasound treatment was beneficial to water absorption, TPC enrichment, energy metabolism, lipid metabolism and protein hydrolysis. Higher contents of GABA (3.29 folds), pyruvic acid (7.63 folds), glycerol (4.88 folds), glutamate (2.02 folds) and glucose (1.32 folds) were obtained due to the antagonistic effect between the 30 w ultrasound treatment and 2.0 % CaCl 2 stress at the 9 h pre-germination, and energy, lipid and protein metabolomic pathways were all involved in the GABA accumulation.
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高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2019
·
IF 5.589
Ting-Kai Zhu,Pei-Pei Du,Li-Juan Zeng,Huixiong Lü,Hai-Ming Zhao,Yan-Wen Li,Ce-Hui Mo,Quan-Ying Cai
Crops can take up and accumulate di- n -butyl phthalate (DBP), an extensively used plasticizer with endocrine disrupting effect, which poses potential risk to human health. Our previous study found the genotype variation in accumulation of DBP by different cultivars of rice ( Oryza sativa L.). Nevertheless, the effect of DBP metabolism in vivo on the accumulation variation among different plant cultivars remains unknown. In this study, metabolism variation of DBP by low ( Fengyousimiao ) and high ( Peizataifeng ) DBP-accumulating cultivars of rice and the key enzymes involving in DBP metabolism in rice plants were investigated using in vivo exposure of rice plants and in vitro exposure of root crude enzyme extracts. Both mono- n -butyl phthalate (MBP) and phthalic acid (PA) were detected as DBP metabolites in all rice tissues ( i.e. , roots, stems, leaves) and crude enzyme extracts with MBP predominance. DBP metabolism occurred simultaneously when DBP uptake with the highest metabolism in roots in vivo . Degradation of DBP in root crude enzyme extracts fitted well with the first order kinetics ( R 2 = 0.49–0.76, P < 0.05). The activity of carboxylesterase (CXE) in root crude enzyme extracts was significantly positively correlated with DBP degradation rates. CXE played an important role in DBP metabolism of rice plants, confirming by the fact that triphenyl phosphate of CXE inhibitor could inhibit DBP metabolism of in vivo and in vitro exposure. This result was further confirmed by in vitro degradation of DBP with the commercial pure CXE. The crude enzyme solution from roots of Fengyousimiao with higher CXE activity had significantly higher DBP degradation rates than that of Peizataifeng . However, Fengyousimiao with lower tolerance to DBP stress and higher inhibition by triphenyl phosphate displayed lower DBP metabolism ability in vivo than Peizataifeng .
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高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2018
·
IF 3.974
Xue-Xue Chen,Yang Wu,Xian-Pei Huang,Huixiong Lü,Hai-Ming Zhao,Ce-Hui Mo,Hui Li,Quan-Ying Cai,Ming-Hung Wong
Di-(2-ethylhexyl) phthalate (DEHP) is a typical endocrine disrupting chemical with relatively high concentrations in agricultural soils of China. Here, a rhizobox experiment was conducted to investigate the variations in microbial community and DEHP dissipation among different soil rhizospheric compartments between low ( Fengyousimiao ) and high ( Peizataifeng ) DEHP-accumulating cultivars of rice ( Oryza sativa L.) grown in DEHP spiked soil (0, 20, 100 mg/kg). The dissipation rates of DEHP in rhizospheric soils of Peizataifeng were generally significantly higher than those of Fengyousimiao, with the highest removal rate in 0–2 mm rhizosphere. The results of Illumina-HiSeq high-throughput sequencing revealed that both bacterial and fungal diversity and community structure were significantly different in rhizospheric soils of the two cultivars. DEHP dissipation rates in 0–2 mm rhizosphere of Peizataifeng were positively correlated with bacterial and fungal diversity. The relative abundance of DEHP-degrading bacterial genera Acinetobacter, Pseudomonas and Bacillus of Peizataifeng was generally higher than those in the same rhizospheric compartment of Fengyousimiao in DEHP treatments, resulting in different rhizospheric DEHP dissipation. Cultivation of Peizataifeng in agricultural soil is promising to facilitate DEHP dissipation and ensure safety of agricultural products.
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高影响因子
FOOD CHEMISTRY
·
2016
·
IF 4.052
Qiang Xia,Liping Wang,Congcong Xu,Jun Mei,Yunfei Li
The effects of germination and high hydrostatic pressure (HHP) processing on the in vitro bioaccessibility of mineral elements, amino acids (AAs), antioxidants and starch in brown rice (BR) were investigated. Germinated BR (GBR) was obtained by incubating at 37 °C for 36 h and then subjected to HHP treatments at 0.1, 100, 300 and 500 MPa for 10 min. The in vitro bioaccessibility of calcium and copper was increased by 12.59–52.17% and 2.87–23.06% after HHP, respectively, but bioaccessible iron was decreased. In addition, HHP significantly improved individual AAs, particularly indispensable AAs and gama-aminobutyric acid, as well as bioaccessible total antioxidant activities and starch resistance to enzymatic hydrolysis. However, germination greatly increased starch digestibility. Atomic force microscopy characterization suggested an obvious structural change in bran fraction at pressures above 300 MPa. These results can help to understand the effects of germination and HHP technologies on nutrients bioaccessibility and develop appropriate processing conditions.
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高影响因子
危险品化学品经营许可证（带存储）