Scientific Reports
·
2015
·Vol. 5
·期 1
·1-10
·
IF 5.578
Xie Jingjing,Wang Jichuang,Chen Hongning,Shen Weiyu,Sinko Patrick J.,Dong Haiyan,Zhao Rongli,Lu Yusheng,Zhu Yewei,Jia Lee
Circulation tumor cells (CTCs) in the bloodstream of early-stage cancer patients carry the important information about valuable biomarkers and biological properties of primary tumor. However, detection and capture of CTCs are challenging owing to their low concentrations. Traditional technologies have the limited detection sensitivity and the low capture efficiency. We, herein, report an effective approach to specifically bind and capture colon cancer HT29 cells by using multiple Sialyl Lewis X antibodies (aSlex)-conjugated PAMAM dendrimers. The conjugation was characterized by using atom force microscope, UV and fluorescence measurements. The capturing and regulating HT29 cells by the aSlex-coated dendrimer conjugate were analyzed by microscopy and flow cytometry. The results indicated that the conjugate showed the enhanced capture of HT29 cells in a concentration-dependent manner and the maximum capture efficiency of 77.88% was obtained within 1 h-exposure. G6-5aSlex-FITC conjugate showed capture efficiency better than FITC-G6-COOH-5aSlex conjugate. G6-5aSlex-FITC conjugate could specifically capture HT29 cells even when the target HT29 cells were diluted with the interfering cells (e.g., RBCs) to a low concentration. The capture resulted in a concentration-dependent restraint of the cell activity. In conclusion, the aSlex-coated dendrimer conjugate displayed the great potential in capturing and restraining colorectal CTCs in blood.
研究领域:
肿瘤学
·
纳米医学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2025
·
IF 8.2
Xue Cai,Yuxing Chen,Jilin Wu,Aijing Wang,Xiaoyan Wang
Root caries present a significant challenge in dentistry. The unsatisfactory prognosis of restorative treatments requires novel, noninvasive preventive strategies. Here, we developed an amelogenin-derived peptide-modified poly(amidoamine), PAMAM-C11, to prevent demineralization in caries lesions and control periodontal destruction. PAMAM can induce dentin remineralization, whereas the C11 peptide strongly binds hydroxyapatite. When C11 is modified on the surface groups of PAMAM, it anchors the entire molecule on demineralized dentin surfaces, thereby resisting washout and enhancing the mineralization efficiency, especially in the presence of collagenase in the cariogenic environment. PAMAM-C11 also inhibits matrix metalloproteinases in dentin and periodontal tissues, protecting the necessary mineralization templates and controlling periodontal destruction. Furthermore, PAMAM-C11 can promote the proliferation and osteogenic differentiation of periodontal ligament stem cells, indicating its potential use in periodontal regeneration. These findings were ultimately validated in an in vivo rat caries model. It can be concluded that PAMAM-C11 has great potential for clinical applications on root caries prevention.
研究领域:
牙科学
·
生物材料学
·
再生医学
✓ 已验证
DOI
PubMed
高影响因子
Journal of the Taiwan Institute of Chemical Engineers
·
2021
·
IF 5.876
Zhihong Yin,Liandong Zhu,Fan Mo,Shuangxi Li,Dan Hu,Ruoyu Chu,Chenchen Liu,Chaozhen Hu
Cu (II) is one of the most harmful heavy metals in the environment, due to the hazardous effects to aquatic organisms, plants, animals, and human beings. In this work, a novel modified magnetic biochar (MBC@APTES-PAMAM) was prepared by PAMAM dendrimer for the removal of Cu (II). The analysis of structural properties identically demonstrated that the abundant -NH 2 groups were successfully loaded onto magnetic biochar surfaces. The sorption kinetics process of Cu (II) onto MBC@APTES-PAMAM was better fitted by the pseudo-second-order model. Langmuir isotherm model was better described for Cu (II) sorption process. MBC@APTES-PAMAM showed outstanding performances on Cu (II) removal, and the maximal adsorption amount was 251.81 mg/g. Adsorption experiment indicated that MBC@APTES-PAMAM had a higher Cu (II) removal efficiency under the pH range from 2 to 8. The adsorption capacity of Cu (II) onto MBC@APTES-PAMAM was decreased in the presence of competing ions. The existence of surfactant and humic acid had great influences on Cu (II) adsorption process. The Cu (II) adsorption mechanism was connected to the -NH 2 by complexation reaction, due to the increased electrostatic attraction and bridging influences. In addition, MBC@APTES-PAMAM also exhibited comparable removal performances for COD (70.82%), TN (71.10%), TP (100%), NH 3 N (55.03%) and Cu (II) (99.68%) in the practical treatment of landfill leachate. Overall, MBC@APTES-PAMAM is a green, low-cost, and high efficiency adsorbent for Cu (II) removal.
研究领域:
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
Materials Science & Engineering C-Materials for Biological Applications
·
2018
·
IF 5.08
Guan-Hai Wang,Hua Chen,Yin-Yu Cai,Li Li,Hui-Kang Yang,Qin Li,Zhi-Jiang He,Jian-Tao Lin
The nucleus is one of the most important cellular organelles, where gene encode and transcribe at that location. However, nucleus-targeting gene delivery are rare been reported. It is important to develop a high-efficiency nucleus-targeting gene vector that can deliver targeted gene into nucleus directly for destroy of cancer cells. Here, special nucleus-targeting and size changeable deliver system based on TAT-SS-PAMAM-D3 with TAT functional on the surface and disulfide linked between D2 and D3 is designed to perform highly efficient nucleus-targeting gene delivery for effective cancer cell killing in vitro. CLSM observations reveal that more TAT-SS-PAMAM-D3 are enter into the nucleus when compare to SS-PAMAM-D3. The TAT modified vector can also act as gene deliver to reach high gene transfection efficiencies, high apoptosis and low viability in HeLa cells. This TAT functionalized and disulfide linking in the carrier may become a prospective vector for cancer gene treatment and also offered a different strategy for designing a better gene delivery system.
研究领域:
遗传学
·
肿瘤学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS
·
2022
·Vol. 22
·期 3
·1075
·
IF 3.847
Xin Wang,Weiguang Shi,Yuda Wang,Dan Cheng,Jiahui Liu,Shihan Xu,Wei Liu,Biao Dong,Jiao Sun
A typical and environment-friendly fluorescent polyamine-amine (PAMAM) features good compatibility and unique surface modification, while it is restricted by a low fluorescence property performance and an unclear fluorescence mechanism. In this work, we prepared blue fluorescent PAMAM polymer dots (PDs) via a simple hydrothermal method based on dichloromethane (DCM) and 2.0G PAMAM. The quantum yield achieved was 32.1%, which was 25 times stronger than that of 2.0G PAMAM due to the lone-pair electron leap of the amine groups, the aggregation of carbonyl groups, as well as the crosslinking induced by DCM inside the PAMAM. In addition, the fluorescent 2.0G PAMAM-DCM PDs show a great Fe3+sensing property with the detection limit of 56.6 nM, which is much lower than the safety limits (5.36 μM) in drinking water, indicating its great potential for Fe3+detection in aqueous media.
研究领域:
材料科学
·
分析化学
·
纳米技术
·
环境监测
✓ 已验证
DOI
高影响因子
Catalysis Science & Technology
·
2019
·Vol. 9
·期 21
·6145-6151
·
IF 5.726
Desheng Yang,Rui Zhang,Ting Zhao,Tingting Sun,Xiaomeng Chu,Shaojie Liu,Erjun Tang,Xiaodong Xu
Due to their small size, uniform dispersion and good stability, dendrimer encapsulated nanoparticles (DENs) are widely used as nanocomposite materials. However, as a commonly used template, the polyamidoamine (PAMAM) dendrimer is limited in the biological and medical fields because of its toxicity. Besides, nanoparticles are commonly encapsulated inside dendrimers, which limits their effective contact with the reactants. In this paper, we presented a comprehensive investigation on the synthesis, characterization and application of 4-carbomethoxypyrrolidone modified polyamidoamine (PAMAM-Pyr) dendrimer–silver nanocomposites (Ag–PPDNCs). The silver nanoparticles were stabilized by PAMAM-Pyr, which combined the advantages of PAMAM and polyvinylpyrrolidone (PVP). Compared with particles prepared with PVP or PAMAM, silver nanoparticles prepared with PAMAM-Pyr had smaller particle sizes with a narrow distribution of 4.78 ± 0.57 nm, which were a kind of mixed type of dendrimer nanocomposite. In addition, Ag–PPDNCs showed a very high activity in the reduction of 4-nitrophenol with an observed rate constant of up to 7.2 × 10−3 s−1 and the turnover frequency was as high as 948.6 h−1, which was superior to that of other reported templates. These results demonstrated the significant role of PAMAM-Pyr in the preparation of Ag–PPDNCs with small particle sizes and excellent catalytic reduction properties. Due to its excellent application performance and low toxicity, PAMAM-Pyr has great application potential in the fields of catalysis and medicine.
研究领域:
纳米技术
·
催化
·
材料科学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
POLYMER COMPOSITES
·
2013
·Vol. 35
·期 4
·627-635
·
IF 1.482
Lei Xia,Baoqing Shentu,Zhixue Weng
The effect of dendrimer-like polyamidoamine grafted attapulgite (ATP-PAMAM) on the microstructure and morphology of Nylon-6 (PA6) was investigated. The ATP-PAMAM nanoparticles were prepared by treating attapulgite (ATP) with heat and acid followed by grafting with polyamidoamine (PAMAM) molecules, which was confirmed by Fourier transform infrared spectroscopy (FT-IR), thermogravimetric analysis (TGA), and dispersion state in formic acid. The X-ray diffraction (XRD) analysis result indicated that the grafting modification was occurred on the surface of fibrous crystals and did not shift the crystal structure of ATP. PA6/ATP-PAMAM (G 2.0 ) nanocomposites with different modified ATP content were prepared by melt compounding in a twin screw extruder. XRD measurements suggested that the intensity of diffraction peak of α crystalline form of PA6 decreased gradually as the inclusion of ATP-PAMAM(G 2.0 ) into the PA6 matrix, while that of γ crystalline form increased gradually. The results of molau experiment and scanning electron microscopy (SEM) observation showed not only a uniform dispersion of ATP-PAMAM(G 2.0 ) in the PA6 matrix but also a strong interfacial adhesion between them. Mechanical investigation (by tensile test) showed an obvious improvement in the presence of surface modified ATP. POLYM. COMPOS., 35:627–635, 2014. © 2013 Society of Plastics Engineers
研究领域:
材料科学
·
高分子化学
✓ 已验证
产品关联
DOI
高影响因子
Gels
·
2022
·Vol. 8
·期 10
·683
·
IF 4.432
Xiangbin Liu,Suling Wang,Weiguang Shi,He Liu
Smart hydrogels, owing to their exceptional viscoelastic and deformable capacity in response to environmental stimulation involving temperature and pH, have been successfully applied in oilfields for purposes such as water and/or gas shutoff treatments. However, the CO2breakthrough problem in low permeability reservoirs has not been well solved. In this work, a rheological method-based Avrami dynamics model and Dickinson dynamics model were employed to investigate the dynamic gelation process of thermo-/pH-dual-sensitive PEG/PAMAM nanogels to further our understanding of the microstructure of their gelation and pertinence plugging application. Plugging experiments were performed by alternating injections of CO2and hydrogel solution in a slug type on three fractured low permeability cores with a backpressure of 13 MPa. The nanogels presented a secondary growth pattern from three to one dimension from micrometer to nanometer size with a morphological transformation from a sphere to an irregular ellipsoid or disk shape. The phase transition temperature was 50 °C, and the phase transition pH was 10. If both or either were below these values, the hydrogel swelled; otherwise, it shrank. Plugging results show that the plugging efficiency was higher than 99%. The maximum breakthrough pressure was 19.93 MPa, and the corresponding residual pressure remained 17.64 MPa for a 10 mD core, exhibiting great plugging performance and high residual resistance after being broken through by CO2.Keywords:smart nanogel;PEG;PAMAM;reaction dynamics;CO2channeling plugging
研究领域:
石油工程
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2015
·
IF 3.368
Jingjing Zhao,Bo Zhang,Shun Shen,Jun Chen,Qizhi Zhang,Xinguo Jiang,Zhiqing Pang
Glioblastoma multiforme (GBM) is the most aggressive central nervous system (CNS) tumor because of its fast development, poor prognosis, difficult control and terrible mortality. Poor penetration and retention in the glioblastoma parenchyma were crucial challenges in GBM nanomedicine therapy. Nanoparticle diameter can significantly influence the delivery efficiency in tumor tissue. Decreasing nanoparticle size can improve the nanoparticle penetration in tumor tissue but decrease the nanoparticle retention effect. Therefore, small nanoparticles with high retention effect in tumor are urgently needed for effective GBM drug delivery. In present study, a small nanoparticle drug delivery system was developed by conjugating fibrin-binding peptide CREKA to Polyamidoamine (PAMAM) dendrimer , where PEGylated PAMAM is used as drug carrier due to its small size and good penetration in tumor and CREKA is used to target the abundant fibrin in GBM for enhanced retention in tumor. In vitro binding ability tests demonstrated that CREKA can significantly enhanced nanoparticle binding with fibrin. In vivo fluorescence imaging of GBM bearing nude mice, ex vivo brain imaging and frozen slices fluorescence imaging further revealed that the CREKA-modified PAMAM achieved higher accumulation and deeper penetration in GBM tissue than unmodified one. These results indicated that the CREKA-modified PAMAM could penetrate the GBM tissue deeply and enhance the retention effect, which was a promising strategy for brain tumor therapy.
研究领域:
肿瘤学
·
药剂学
·
神经科学
✓ 已验证
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Dandan Guo,Xiaoqian Zhou,Shaohua Huang,Yan Zhu
In this work, achiral metal organic frameworks (MIL-53(Al)–NH 2 ) were synthesized and functionalized with poly (amidoamine) (PAMAM) dendrimers. By dispersing PAMAM grafted MIL-53(Al)–NH 2 to water, the obtained solution exhibited high fluorescence intensity and stability. Due to the strong affinity interaction, fluorescence of the solution was quenched when Cu 2+ was added. Following the addition of lysine to the system, the quenched fluorescence intensity was partially restored based on the chelation between Cu 2+ and lysine. In this case, the fluorescent signal of MIL-53(Al)–NH 2 -PAMAM-Cu 2+ system became turn-on state. Furthermore, because of different chelating abilities with L-lysine and D-lysine, the system exhibited enantioselective fluorescence enhancement. Therefore, the PAMAM grafted MIL-53(Al)–NH 2 were successfully used for enantioselective fluorescence detection of lysine after being quenched by Cu 2+ . The method showed good linearity in both L/D-lysine detection (R 2 = 0.9976–0.9984) and ee values determination (R 2 = 0.9921). The detection limit of L-lysine and D-lysine were 7.52 and 12.2 μmol L −1 , respectively. Reliability of the method was verified by analysis of lysine enantiomers in spiked drinking water samples. Rapid enantioselective fluorescent imaging of lysine enantiomers showed great application prospects in test paper, diagnostic kit and vivo imaging system.
研究领域:
分析化学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2023
·
IF 9.5
Mengen Ma,Yilin Zeng,Yuzhao Yang,Cuiling Zhang,Yujiao Ma,Shaohang Wu,Chong Liu,Yaohua Mai
The wide-band-gap inorganic CsPbI2Br perovskite material provides a highly matched absorption range with the indoor light spectrum and is expected to be used in the fabrication of highly efficient indoor photovoltaic cells (IPVs) and self-powered low-power Internet of Things (IoT) sensors. However, the defects that cause nonradiative recombination and ion migration are assumed to form leakage loss channels, resulting in a severe impact on the open-circuit voltage (VOC) and the fill factor (FF) of IPVs. Herein, we introduce poly(amidoamine) (PAMAM) dendrimers with multiple passivation sites to fully repair the leakage channels in the devices, taking into account the characteristics of IPVs that are extremely sensitive to nonradiative recombination and shunt resistance. The as-optimized IPVs demonstrate a promising PCE of 35.71% under a fluorescent light source (1000 lux), with VOC increased from 0.99 to 1.06 V and FF improved from 75.21 to 84.39%. The present work provides insight into the photovoltaic mechanism of perovskites under full sun and indoor light, which provides guidance for perovskite photovoltaic technology with industrialization prospects.
研究领域:
材料科学
·
能源技术
·
光电子学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2016
·
IF 7.476
Yin-Zhu Wang,Wei Zhao,Pan-Pan Dai,Hai-Jie Lu,Jing-Juan Xu,Jing Pan,Hong-Yuan Chen
Herein, a spatial-resolved electrochemiluminescene (ECL) ratiometry based on a closed biopolar electrode (BPE) is reported for the highly sensitive detection of prostate specific antigen (PSA). [email protected] 3 N 4 NCs as one ECL emitter were firstly coated on the cathode of BPE, while the anode of the BPE served for calibration via another ECL substance, Ru(bpy) 3 2+ . The electroneutrality across the BPE makes the reactions on each pole of BPE electrically coupled. Thus one electrochemical sensing reaction at one pole of BPE could be quantified at both ends. A composite, Pt-PAMAM-DNAzyme was assembled on the surface of cathode via DNA hybridization between probe DNA and PSA aptamer . It acted as an ECL quencher of g-C 3 N 4 via resonance energy transfer (RET) and catalyzing the reduction of O 2 , the co-reactant of g-C 3 N 4 . Meanwhile, it could promote the ECL of Ru(bpy) 3 2+ at anode, since the catalytic reduction of O 2 at the cathode increased the faradiac current flowing through the BPE. Based on this signal composite , an ECL “off-on” phenomenon was observed at the cathode, after Pt-PAMAM-DNAzyme was “peeled off” by PSA. Conversely, at the anode, an “on-off” ECL changing was obtained. Therefore, a sensitive ratiometry for PSA detection was achieved with a linear range from 0.10 to 200 ng/mL. Since the two ECL emitters were physically separated, the ratiometric system was relatively simple and neither optical filters nor spectrometer were required. The strategy combining the ECL ratiometry and BPE broadens the applications of BPE-ECL and shows good perspective in clinical application.
研究领域:
生物分析
·
医学检测
·
电化学传感
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）