International Journal of Nanomedicine
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2018
·
IF 4.37
Fei Tong,Rongkui Chai,Haiying Jiang,Bo Dong
Background The objective of this study was to survey the therapeutic function of curcumin-encapsulated poly(gamma-benzyl l-glutamate)-poly(ethylene glycol)-poly(gammabenzyl l-glutamate) (PBLG-PEG-PBLG) (P) on diabetic cardiomyopathy (DCM) via cross regulation effect of calcium-sensing receptor (CaSR) and endogenous cystathionine-γ-lyase (CSE)/hydrogen sulfide (H 2 S). Methods Diabetic rats were preconditioned with 20 mg/kg curcumin or curcumin/P complex continuously for 8 weeks. The blood and myocardiums were collected, the level of serum H 2 S was observed, and the [Ca 2+ ] i content was measured in myocardial cells, and hematoxylin-eosin, CaSR, CSE, and calmodulin (CaM) expression were detected. Results Both curcumin and curcumin/P pretreatment alleviated pathological morphological damage of myocardium, increased H 2 S and [Ca 2+ ] i levels, and upregulated the expression of CaSR, CSE, and CaM as compared to DCM group, while curcumin/P remarkably augmented this effect. Conclusion PBLG-PEG-PBLG could improve water-solubility and bioactivity of curcumin and curcumin/PBLG-PEG-PBLG significantly alleviated diabetic cardiomyopathy.
研究领域:
药理学
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心血管疾病
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纳米医学
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产品关联
DOI
PubMed
高影响因子
MARINE POLLUTION BULLETIN
·
2023
·
IF 5.8
Chen Zhang,Yuhong Qi,Yarui Guo,Shukun Zhang,Gang Xiong,Kaixuan Wang,Zhanping Zhang
Polystyrene microspheres compounded with polyethylene glycol–based hydrogel (PS-PEG)/polydimethylsiloxane (PDMS) coatings were prepared using the physical blending method. The chemical structure, surface and interface properties, interlayer adhesion, and tensile properties were tested in this paper. Furthermore, the antifouling performance was evaluated through bovine serum albumin fluorescent protein adsorption testing, marine bacteria adhesion testing, and benthic diatom adhesion testing. The results showed that the coating performance was best when 20 wt% PS-PEG hydrogel was added. Its surface energy was only 19.21 mJ/m 2 , the maximum breaking strength was 1.24 MPa, the maximum elongation rate was 675 %, the elastic modulus was 2.53 MPa, and the anti-stripping rate was 100 %. In addition, the coating with added 20 wt% PS-PEG hydrogel bacterial adherence rate was 5.36 % and 2.45 % after rinsing and washing, respectively, and the removal rate was 54.29 %. In the benthic diatom adhesion test , the chlorophyll concentration a-value was only 0.0017 mg/L after washing with added 20 wt% hydrogel, and the protein desorption rate was 84.19 % higher than PDMS in the fluorescent protein adsorption test. This coating has the ‘low adhesion’ and ‘desorption’ characteristics in the three growth stages of biofouling. Meanwhile, the low surface energy of the silicone is stable, and the hydrogel also dynamically migrates to the surface to gradually form a hydration layer, both are synergistic. When 20 wt% PS-PEG hydrogel was added, the coating demonstrated excellent antifouling performance due to its high hydration layer, low surface energy, high elasticity, and high interlayer adhesion. This research is expected to contribute to the practical applications of hydrogel coatings in marine antifouling.
研究领域:
材料科学
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海洋生物学
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
BIOMATERIALS
·
2018
·
IF 8.806
Jie Li,Chunyang Sun,Wei Tao,Ziyang Cao,Haisheng Qian,Xianzhu Yang,Jun Wang
Controlling poly(ethylene glycol) (PEG) shielding/deshielding at the desired site of action exhibits great advantages for nanocarrier-based on-demand drug delivery in vivo . However, the current PEG deshielding strategies were mainly designed for anticancer drug delivery; even so, their applications are also limited by tumor heterogeneity. As a proof-of-concept, we explored a photoinduced PEG deshielding nanocarrier TK-NP Ce6&PTX to circumvent the aforementioned challenge. The TK-NP Ce6&PTX encapsulating chlorin e6 (Ce6) and paclitaxel (PTX) was self-assembled from an innovative thioketal (TK) linkage-bridged diblock copolymer of PEG with poly(d, l -lactic acid) (PEG- TK -PLA). We demonstrated that the high PEGylation of TK-NP Ce6&PTX in blood helps the nanocarrier efficiently avoid rapid clearance and consequently prolongs its circulation time . At the desired site (tumor), 660-nm red light irradiation led to ROS generation in situ , which readily cleaved the TK linkage, resulting in PEG deshielding. Such photoinduced PEG deshielding at the desired site significantly enhances the cellular uptake of the nanocarriers, achieving on-demand drug delivery and superior therapeutic efficacy. More importantly, this strategy of photoinducing PEG deshielding of nanocarriers could potentially extend to a variety of therapeutic agents beyond anticancer drugs for on-demand delivery.
研究领域:
药剂学
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生物医学工程
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肿瘤学
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DOI
PubMed
高影响因子
LAB ON A CHIP
·
2024
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IF 6.1
Wenwen Chen,Hao Li,Xinyue Zhang,Yutao Sang,Zhihong Nie
Monodisperse biodegradable polymer microspheres show broad applications in drug delivery and other fields. In this study, we developed an effective method that combines microfluidics with interfacial instability to prepare monodispersed poly(lactic-co-glycolic acid)-b-polyethylene glycol (PLGA-PEG)/poly(lactic-co-glycolic acid) (PLGA) microspheres with tailored surface morphology. By adjusting the mass ratio of PLGA-PEG to PLGA, the concentration of stabilizers and the type of PLGA, we generated microspheres with various unique folded morphologies, such as “fishtail-like”, “lace-like” and “sponge-like” porous structures. Additionally, we demonstrated that risperidone-loaded PLGA-PEG/PLGA microspheres with these folded morphologies significantly enhanced drug release, particularly in the initial stage, by exhibiting a logarithmic release profile. This feature could potentially address the issue of delayed release commonly observed in sustained-release formulations. This study presents a straightforward yet effective approach to construct precisely engineered microspheres offering enhanced control over drug release dynamics.
研究领域:
药剂学
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材料科学
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药物递送系统
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DOI
PubMed
高影响因子
LANGMUIR
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2024
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IF 3.7
Jiacong Wu,Shiyuan Liu,Xinnan Ma,Cailiang Zhang,Chengxiang Feng,Lei Wang,Juan Han,Yun Wang
The current study presents a scalable approach for the preparation of temperature-responsive PEG/SiO2/PNIPAM-PEA Janus particles and, for the first time, investigates their potential applications in stabilizing foam and defoaming by adjusting the temperature. The method utilizes a (W1 + O)/W2 emulsion system, which incorporates appropriate surfactants to stabilize the emulsion and prevent rapid dissolution of the hydrophilic triblock polymer PEG-b-PTEPM-b-PNIPAM in water. The PEG/SiO2/PNIPAM-PEA Janus particles with temperature-responsive characteristics were synthesized in a single step that combined the sol–gel reaction and photoinduced free radical polymerization. The contact angle of the hydrophilic PEG/SiO2/PNIPAM surface was measured to be 54.7 ± 0.1°, while the contact angle of the hydrophobic PEA surface was found to be 122.4 ± 0.1°. By incorporating PEG/SiO2/PNIPAM-PEA Janus particles at a temperature of 25 °C, the foam’s half-life is significantly prolonged from 42 s to nearly 30 min. However, with an increase in temperature to 50 °C, the foam’s half-life rapidly diminished to only 44 s. This innovative application effectively enhances foam stabilization at low temperatures and facilitates the rapid dissipation of foam at high temperatures.
研究领域:
材料科学
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化学工程
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胶体与表面化学
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产品关联
DOI
PubMed
高影响因子
Polymers
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2022
·Vol. 14
·期 9
·1917
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IF 4.967
Fue Ren,Shuyan Yang,Yang Wu,Feng Guo,Feng Zhou
An ultra-low friction and high load capacity could be obtained on the surfaces grafted by a polymer brush even at relatively slow friction speeds in aqueous lubrication environments, which has attracted widespread attention to study the lubrication mechanism of polymer brushes; however, it has yet to be fully understood. The macroscopic tribological performance of two different polymer brushes, which were prepared by the method of “grafting to” and “grafting from” and named as PLL-g-PEG and PSPMA, respectively, were investigated. The friction results demonstrated that PLL-g-PEG obtained a lower friction coefficient than polymer brush PSPMA, which was ascribed to its unique “self-healing” behavior. The lubrication film was in situ observed and the film thickness induced by the polymer brush was measured using a laboratory set for film thickness measurement apparatus based on interference technology. It was found that PSPMA exhibited excellent lubrication performance not found in PLL-g-PEG, and two film-forming mechanisms highly dependent on velocity were revealed, which may be important to interpret the lubrication mechanism of polymer brushes in aqueous lubricants.Keywords:PLL-g-PEG brushes;PSPMA brushes;friction;film formation property;hydration effect;hydrodynamic effect;lubrication mechanism
研究领域:
摩擦学
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聚合物科学
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材料科学
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产品关联
DOI
PubMed
高影响因子
Materials
·
2023
·Vol. 16
·期 6
·2277
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IF 3.4
Si Xu,Shanshan Liang,Bing Wang,Jiali Wang,Meng Wang,Lingna Zheng,Hao Fang,Tingfeng Zhang,Yi Bi,Weiyue Feng
Pre-coating with a protein corona on the surface of nanomaterials (NMs) is an important strategy for reducing non-specific serum protein absorption while maintaining targeting specificity. Here, we present lipoic acid-terminated polyethylene glycol and transferrin bi-functionalized MoS2nanosheets ([email protected]2-PEG NSs) as a feasible approach to enhance cellular uptake.[email protected]2-PEG NSs can maintain good dispersion stability in cell culture medium and effectively protect MoS2NSs from oxidation in ambient aqueous conditions. Competitive adsorption experiments indicate that transferrin was more prone to bind MoS2NSs than bovine serum albumin (BSA). It is noteworthy that single HepG2 cell uptake of[email protected]2-PEG presented a heterogeneous distribution pattern, and the cellular uptake amount spanned a broader range (from 0.4 fg to 2.4 fg). Comparatively, the intracellular Mo masses in HepG2 cells treated with[email protected]2-PEG and MoS2-PEG showed narrower distribution, indicating homogeneous uptake in the single HepG2 cells. Over 5% of HepG2 cells presented uptake of the[email protected]2-PEG over 1.2 fg of Mo, about three-fold that of[email protected]2-PEG (0.4 fg of Mo). Overall, this work suggests that Tf coating enhances the cellular uptake of MoS2NSs and is a promising strategy for improving the intracellular uptake efficiency of cancer cells.Keywords:bi-functionalization;[email protected]2-PEG nanosheets;HepG2 cellular uptake
研究领域:
材料科学
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纳米技术
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生物医学工程
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细胞生物学
✓ 已验证
DOI
PubMed
高影响因子
REACTIVE & FUNCTIONAL POLYMERS
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2016
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IF 2.725
Guiyou Wang,Lingmei Zhang
In this study, well-defined double-hydrophilic triblock copolymers , consisting of hydrophilic poly(ethylene glycol) (PEG) and hydrophobic poly( N , N -diethylamino-2-ethylmethacrylate) (PDEAEMA), were synthesized and characterized. The molecular weights of the copolymers were controllable. In the basic environments, the copolymers can self-assemble into the PDEAEMA core and PEG corona micelles in aqueous solution with an average size of 40–180 nm. The low critical micelle concentration (CMC, about 10 − 3 mg mL − 1 ) of copolymers investigated in pH 7.4 aqueous solution confirmed the adequate thermodynamic stability of their self-assembly. As shown in the cytotoxicity assay, the copolymers have good biocompatibility. Doxorubicin (DOX) was encapsulated into the triblock copolymer micelles . The drug loading efficiency and entrapment efficiency values of DOX-loaded micelles reached 8.14% and 88.6%, respectively. The DOX release of the drug-loaded micelles depends on the pH values. These phenomena indicated that the double-hydrophilic PDEAEMA – PEG – PDEAEMA triblock copolymers have a potential application in the drug delivery.
研究领域:
材料科学
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药学
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生物医学工程
✓ 已验证
DOI
高影响因子
ADVANCES IN POLYMER TECHNOLOGY
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2020
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IF 1.539
Lei Li,Yan Li,Cheng Miao,Rui Liu
This study was to explore the therapeutic effect and mechanism of puerarin (PUE) combined with PEGylated nanoparticles on a rat cerebral infarction cell model. In this context, PEG-PLGA/PUE nanoparticles were prepared by the thin-film hydration method, and the toxicity of PEG-PLGA/PUE nanoparticles to brain capillary endothelial cell (BCEC) was detected by MTT. The BCEC/TF cell model was obtained by induction of BCEC cells with TNF-α. The BCEC/TF cell model was identified by immunofluorescence; the protein expression was detected by western blotting; the expression level of miR-424 in cells was measured by RT-qPCR; the targeting relationship between miR-424 and PDCD4 was confirmed by dual-luciferase reporter assay. We found that PEG-PLGA/PUE nanoparticles prepared by the thin-film hydration method had uniform particle size, regular shape, and good stability and were not toxic to cells. The vWF was widely expressed in the cytoplasm in BCECs. The BCEC/TF cell model was obtained after TNF-α treatment, and tissue factor (TF) was widely expressed on the cell membrane of BCEC/TF cells. Furthermore, it was observed that the PEG-PLGA/PUE nanoparticles showed better therapeutic effect on the BCEC/TF cell model than PUE. PEG-PLGA/PUE nanoparticles and PUE inhibited the expression of PDCD4 protein by increasing the expression of miR-424 in BCEC/TF cells. In summary, the therapeutic effect of PEG-PLGA/PUE nanoparticles on the in vitro cell model of cerebral infarction is better than that of PUE. Moreover, PEG-PLGA/PUE inhibits the expression of PDCD4 protein by lowering the expression level of miR-424 in cells, thereby reducing the hazard of cerebral infarction.
研究领域:
药理学
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神经科学
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分子生物学
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产品关联
DOI
高影响因子
BIOMACROMOLECULES
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2023
·
IF 6.2
Lan Wang,Zhen Du,Mengmeng Xu,Qiuju Dai,Qing-Yun Guo,Baoer Fan,Wen Tang
Polymeric vehicles often exhibit batch-to-batch variations due to polydispersity, limiting their reproducibility for biomedical applications. In contrast, polyhedral oligomeric silsesquioxane (POSS) has emerged as an attractive candidate for drug delivery due to its precise chemical structure and rigid molecular shape. A promising strategy to enhance drug efficacy while reducing systemic toxicity is the development of multi-stimuli-responsive delivery systems capable of targeted drug release at a disease site. Herein, we developed a drug delivery platform based on POSS–polymer conjugates. By functionalizing the POSS with amino groups and establishing B–N coordination with boronic acids, the nanoparticles (NPs) exhibit responsive behavior to stimuli, including adenosine-5′-triphosphate (ATP), acidic pH, and nucleophilic reagents. We successfully encapsulated two boronic acid-containing molecules: tetraphenylethylene (TPE), serving as a fluorescent probe, and bortezomib (BTZ), an anticancer drug. The TPE@NPs were employed to visualize the cellular uptake of NPs by tumor cells, while the BTZ@NPs exhibited increased cytotoxicity in tumor cells compared with normal cells. This POSS–PEG conjugate offers a nanoparticle platform for encapsulating versatile boronic acid-containing molecules, thereby enhancing drug efficacy while minimizing systemic toxicity. Given the wide-ranging applications of boronic acid-containing molecules in biomedicine, our platform holds significant promise for the development of intelligent drug delivery systems for diagnostics and therapeutics.
研究领域:
药剂学
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生物医学工程
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肿瘤学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TRIBOLOGY INTERNATIONAL
·
2024
·
IF 6.1
Changhong Lai,Song Wang,Pian Zhong,Yanyan Chen,Juan Xu,Xiaoli Li,Weiqiang Liu
Vascular interventional catheters are generally made of hydrophobic polymers , which may lead to vascular injury, poor lubrication and other consequences due to friction with vascular wall or stent in the process of intervention. In this study, the PDA-PVP/PEG composite coating was prepared based on common TPU catheter material to improve its hydrophilic lubrication behavior. Firstly, the oxidative self-polymerization of dopamine (PDA) was initiated and deposited on the surface of TPU in a weak alkali environment, and then the PVP/PEG coating was prepared by UV curing with PDA as the intermediate adhesion layer . The composite coating was optimized step by step by lubricating material system, process parameters, molecular weight , and concentration. Compared with the blank control group, the water contact angle decreased from 91.3° to about 28.3°, and the friction coefficient decreased from 0.4 to about 0.03, and the composite coating was not damaged after 30 min of reciprocating sliding. The results of cytotoxicity grade Level 1 and hemolysis rate less than 5 % also proved that the composite coating showed good cytotoxicity and blood compatibility. In summary, the PDA-PVP/PEG hydrophilic lubricating composite coating revealed excellent comprehensive properties.
研究领域:
生物医学工程
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
·
2016
·Vol. 134
·期 10
·
IF 1.866
Fei Fang,Fan Kong,Xueqin Zhang,Mengqi Lin,Yichi Zhang,Shounian Ding
A series of amphiphilic poly(N-vinyl carbazole) (PVK) block copolymers containing tris(8-hydroxyquinoline) aluminum (Alq 3 ) have been synthesized via reversible addition-fragmentation chain transfer (RAFT) radical polymerization with trithiocarbonate terminated polyethylene glycol (PEG) as a RAFT agent. The chemical structures of the block copolymers have been identified by 1 H nuclear magnetic resonance and Fourier transform infrared spectrometer. The analysis of gel permeation chromatography indicates that the polydispersity indices of the block copolymers are lower than 1.30. The results of thermogravimetric analysis show that the decomposition temperatures of the copolymers are higher than 330 ° C . The optical properties of the copolymers have been investigated and the obviously enhanced emission from Alq 3 has been found due to resonance energy transfer from PVK to Alq 3 . © 2016 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2017 , 134 , 44573.
研究领域:
高分子化学
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材料科学
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光电子学
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纳米功能材料
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聚合物合成
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荧光材料
·
能量转移
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）