RSC Advances
·
2024
·Vol. 14
·期 14
·9848-9859
·
IF 3.9
Shangbo Li,Zijian Liu,Xiaohan Gao,Lidi Cheng,Zexian Xu,Li Li,Yaru Diao,Liqiang Chen,Yanshan Liu,Jian Sun
Jaw defects, which can result from a multitude of causes, significantly affect the physical well-being and psychological health of patients. The repair of these infected defects presents a formidable challenge in the clinical and research fields, owing to their intricate and diverse nature. This study aims to develop a personalized bone tissue engineering scaffold that synergistically offers antibacterial and osteogenic properties for treating infected maxillary defects. This study engineered a novel temperature-sensitive, sustained-release hydrogel by amalgamating β-cyclodextrin (β-CD) with chlorhexidine (CHX) and a decellularized extracellular matrix (dECM). This hydrogel was further integrated with a polylactic acid (PLA)–nano hydroxyapatite (nHA) scaffold, fabricated through 3D printing, to form a multifaceted composite scaffold (nHA/PLA/dECM/β-CD–CHX). Drug release assays revealed that this composite scaffold ensures prolonged and sustained release. Bacteriological studies confirmed that the β-CD–CHX loaded scaffold exhibits persistent antibacterial efficacy, thus effectively inhibiting bacterial growth. Moreover, the scaffold demonstrated robust mechanical strength. Cellular assays validated its superior biocompatibility, attributed to dECM and nHA components, significantly enhancing the proliferation, adhesion, and osteogenic differentiation of osteogenic precursor cells (MC3T3-E1). Consequently, the nHA/PLA/dECM/β-CD–CHX composite scaffold, synthesized via 3D printing technology, shows promise in inducing bone regeneration, preventing infection, and facilitating the repair of jaw defects, positioning itself as a potential breakthrough in bone tissue engineering.
研究领域:
生物材料学
·
组织工程学
·
口腔医学
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产品关联
DOI
PubMed
高影响因子
ACS Applied Polymer Materials
·
2025
·
IF 4.7
Yaru Diao,Li Li,Yaoxiang Xu,Yanshan Liu,Ruxi Sun,Liqiang Chen,Yupeng Wu,Xiaohan Gao,Wenhao Cheng,Chen Chen,Zexian Xu,Jian Sun
In bone tissue reconstruction, infection is a critical limiting factor affecting treatment efficacy. So it is necessary to introduce antibacterial agents onto scaffold surfaces for functional modification while balancing osteoblast activity. Carbon nanotubes (CNTs), with their exceptional osteogenic properties and drug-loading capacity, emerge as ideal materials. Digital 3D printing technology was employed to composite CNTs loaded with the antibacterial agent chlorhexidine gluconate (CG) with polylactic acid-nano hydroxyapatite (PLA/nHA), and a novel composite scaffold with a controllable porous structure (PLA/nHA/CNTs-CG) was successfully constructed. TEM observations revealed the hollow structure of CNTs, while XPS, XRD, N2 adsorption–desorption tests, and TGA confirmed successful CG loading. SEM analysis demonstrated the porous network-like structure of PLA/nHA/CNTs-CG scaffolds, exhibiting compressive strength of (8.44 ± 0.24) MPa. Initial biosafety validation through CCK-8 assay, live/dead cell staining, and hemocompatibility tests confirmed good biocompatibility. Cell adhesion experiments demonstrated enhanced cellular attachment on PLA/nHA/CNTs-CG scaffolds. Osteogenic performance tests revealed that CNTs incorporation improved MC3T3-E1 cell osteogenic differentiation, with CG presence not compromising scaffold osteogenic activity. Antibacterial experiments demonstrated potent antibacterial effects against both Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli). The PLA/nHA/CNTs-CG composite scaffold demonstrates dual functionality in infectious bone defect repair by simultaneously promoting bone regeneration and exerting antibacterial effects, providing novel insights for designing bone repair materials for infected defects.
研究领域:
生物材料学
·
再生医学
·
骨科
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DOI
高影响因子
ACS Applied Materials & Interfaces
·
2017
·
IF 7.504
Dishi Wang,Yanxia Zhou,Xinru Li,Xiaoyou Qu,Yunqiang Deng,Ziqi Wang,Chuyu He,Yang Zou,Yiguang Jin,Yan Liu
pH-responsive polymeric micelles have shown promise for the targeted and intracellular delivery of antitumor agents. The present study aimed to elucidate the possible mechanisms of pH-sensitivity and cellular internalization of PEOz-b-PLA micelles in detail, further unravel the effect of hydrophilic/hydrophobic ratio of the micelles on their cellular internalization, and examine the intracellular trafficking routes and fate of PEOz-b-PLA after internalization of the micelles. The results of variations in the size and Zeta potential of PEOz-b-PLA micelles and cross-sectional area of PEOz-b-PLA molecules with pH values suggested that electrostatic repulsion between PEOz chains resulting from ionization of the tertiary amide groups along PEOz chain at pH lower than its pKa was responsible for pH-sensitivity of PEOz-b-PLA micelles. Furthermore, the studies on internalization of PEOz-b-PLA micelles by MCF-7 cells revealed that the uptake of PEOz-b-PLA micelles was strongly influenced by their structural features, and showed that PEOz-b-PLA micelles with hydrophilic/hydrophobic ratio of 1.7–2.0 exhibited optimal cellular uptake. No evident alteration in cellular uptake of PEOz-b-PLA micelles was detected by flow cytometry upon the existence of EIPA and chlorpromazine. However, the intracellular uptake of the micelles in the presence of MβCD and genistein was effectively inhibited. Hence, the internalization of such micelles by MCF-7 cells appeared to proceed mainly through caveolae/lipid raft-mediated endocytosis without being influenced by their hydrophilic/hydrophobic ratio. Confocal micrographs revealed that late endosomes, mitochondria and endoplasmic reticulum were all involved in the intracellular trafficking of PEOz-b-PLA copolymers following their internalization via endocytosis, and then part of them was excreted from tumor cells to extracellular medium. These findings provided valuable information for developing desired PEOz-b-PLA micelles to improve their therapeutic efficacy and reducing the potential safety risks associated with their intracellular accumulation.
研究领域:
药剂学
·
分子生物学
·
肿瘤学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Xiaoying Zhao,Tianyu Chen,Jiaxin Liu,Xinning Wang,Yunxuan Weng
Microbial contamination is the leading cause of food spoilage and food-borne disease. Here, we developed a multifunctional surface based on polylactic acid (PLA) bioplastic with antifouling and antibacterial properties via a facile dual-coating approach. The surface was designed with hierarchical micro/nano-scale roughness and low surface energy. Bactericidal agent polyhexamethylene guanidine hydrochloride (PHMG) was incorporated to endow the film with bactericidal activity. The film had good superhydrophobic, antifouling and antibacterial performance, with a water contact angle of 154.3°, antibacterial efficiency against E. coli and S. aureus of 99.9 % and 99.6 %, respectively, and biofilm inhibition against E. coli and S. aureus of 63.5 % and 68.9 %, respectively. Synergistic effects of antibacterial adhesion and contact killing of bacteria contributed to the significant antibacterial performance of the film. The biobased biodegradable film was highly effective in preventing microbial growth when applied as antibacterial food packaging for poultry product, extending the shelf life of fresh chicken breast up to eight days.
研究领域:
材料科学
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食品科学
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生物技术
·
化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
·
2023
·Vol. 140
·期 19
·e53839
·
IF 3
Zhengwei Wan,Meili Li,Yaozhong Zhuang,Zaizai Tong
Poly( L -lactic acid) (PLLA) is a biodegradable polymer that has attracted increasing attention due to its fantastic properties. Nevertheless, the disadvantages of PLLA, such as low crystallization rate and brittleness, limit its wide commercial applications. In the present work, electrospun stereocomplex PLA fibers (Sc-fibers) and PLLA grafted cellulose nanocrystals (g-CNCs) are prepared as nano-fillers and then blended with PLLA matrix to prepare different nanocomposites. Both of nano-fillers would accelerate the crystallization rate of PLLA, and as expected, g-CNCs have much stronger nucleation ability towards PLLA than Sc-fibers. Nevertheless, the crystallization of PLLA will decrease to some extent in the presence of both nano-fillers of g-CNCs and Sc-fibers due to the preferential formation of stereocomplex crystals on the surfaces of CNCs. Moreover, the presence of nano-fillers would enhance the mechanical properties of PLLA, such as the elongation at break can increase by four times after incorporating both g-CNCs and Sc-fibers. This work provides a new consideration for designing different nano-fillers, especially for polymer matrices with low crystallization ability and brittleness.
研究领域:
材料科学
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高分子科学
·
纳米技术
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产品关联
DOI
高影响因子
IONICS
·
2017
·Vol. 24
·期 3
·787-795
·
IF 2.062
Wang Ping,Zhang Di,Zhou Yiyang,Li Yu,Fang Huagao,Wei Haibing,Ding Yunsheng
A novel biodegradable 1,2,3-triazolium-functionalized PEG- b -PCL copolymer (TAPEC) was synthesized by the “click” coupling of methoxypolyethylene glycol azide and α-propargyl-ω-hydroxyl-poly(ε-caprolactone), followed by the quaternization of the 1,2,3-triazole moiety with iodomethane. All the intermediates and TAPEC were characterized by 1 H NMR, FT-IR, and gel permeation chromatography (GPC). Taking advantage of the characteristics of ionic liquid and block copolymer, this ion-containing diblock copolymer is expected to be used as a novel compatibilizer in mixed biopolyester for regulating the interface and crystallization behaviors. Hence, the TAPEC was evaluated as a compatibilizer and an interface emulsifier in the blends of polylactic acid (PLA) and poly(ε-caprolactone) (PCL). Non-isothermal crystallization experimental results showed that the TAPEC with the higher amount of ε-caprolactone units induces a plasticization and nucleate effect that increased the crystallization ability of the PLA phase; meanwhile, in the PCL phase, the agminated ionic cluster acting as a nucleating agent significantly increased the crystalline of PCL.
研究领域:
材料科学
·
高分子化学
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产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2016
·
IF 7.145
Xingye Feng,Di Jiang,Ting Kang,Jianhui Yao,Yixian Jing,Tianze Jiang,Jingxian Feng,Qianqian Zhu,Qingxiang Song,Nan Dong,Xiaoling Gao,Jun Chen
The combination of photodynamic therapy (PDT) and chemotherapy holds great potential in combating drug-resistant cancers. However, the major challenge that lies ahead is how to achieve high coloading capacity for both photosensitizer and chemo-drugs and how to gain efficient delivery of drugs to the drug-resistant tumors. In this study, we prepared a nanovehicle for codelivery of photosensitizer (pyropheophorbide-a, PPa) and chemo-drugs (paclitaxel, PTX) based on the synthesis of PPa-conjugated amphiphilic copolymer PPa-PLA-PEG-PLA-PPa. The obtained nanoparticles (PP NP) exhibited a satisfactory high drug-loading capacity for both drugs. To achieve effective tumor-targeting therapy, the surface of PP NP was decorated with a tumor-homing and penetrating peptide F3. In vitro cellular experiments showed that F3-functionalized PP NP (F3-PP NP) exhibited higher cellular association than PP NP and resulted in the strongest antiproliferation effect. In addition, compared with the unmodified nanoparticles, F3-PP NP exhibited a more preferential enrichment at the tumor site. Pharmacodynamics evaluation in vivo demonstrated that a longer survival time was achieved by the tumor-bearing mice treated with PP NP (+laser) than those treated with chemotherapy only or PDT only. Such antitumor efficacy of combination therapy was further improved following the F3 peptide functionalization. Collectively, these results suggested that targeted combination therapy may pave a promising way for the therapy of drug-resistant tumor.
研究领域:
肿瘤学
·
药学
·
纳米技术
·
癌症治疗
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产品关联
DOI
PubMed
高影响因子
International Journal of Nanomedicine
·
2022
·
IF 7.033
Yanan Shi,Wan Huang,Rongcai Liang,Kaoxiang Sun,Fangxi Zhang,Wanhui Liu,Youxin Li
To improve the pharmacokinetics and stability of recombinant human erythropoietin (rhEPO), rhEPO was successfully formulated into poly(ethylene glycol)–poly(d,l-lactide) (PEG–PLA) di-block copolymeric micelles at diameters ranging from 60 to 200 nm with narrow polydispersity indices (PDIs; PDI < 0.3) and trace amount of protein aggregation. The zeta potential of the spherical micelles was in the range of −3.78 to 4.65 mV and the highest encapsulation efficiency of rhEPO in the PEG–PLA micelles was about 80%. In vitro release profiles indicated that the stability of rhEPO in the micelles was improved significantly and only a trace amount of aggregate was found. Pharmacokinetic studies in rats showed highly enhanced plasma retention time of the rhEPO-loaded PEG-PLA micelles in comparison with the native rhEPO group. Increased hemoglobin concentrations were also found in the rat study. Native polyacrylamide gel electrophoresis results demonstrated that rhEPO was successfully encapsulated into the micelles, which was stable in phosphate buffered saline with different pHs and concentrations of NaCl. Therefore, PEG–PLA micelles can be a potential protein drug delivery system.
研究领域:
药学
·
生物技术
·
药物递送系统
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产品关联
DOI
PubMed
高影响因子
COMPOSITE STRUCTURES
·
2025
·
IF 7.1
He Ou-Yang,Xuanyu Zhou,Yulong Zhang,Yan Wang,Ziqiang Zhu,Jianan Bai,Wei jiang,Yanan Zhang,Yubing Hu
The interfacial properties between the components of a composite material influence the overall material properties. Herein, we proposed a “rigid-flexible” approach to improve the relatively weak interlaminar characteristic of 3D printed CF/PLA composite. Graphene oxide (GO) and polyethyleneimine (PEI) were chosen as the “rigid” and “flexible” phases, respectively. A stepwise chemical grafting technique was used to introduce “rigid-flexible” structures to the continuous carbon fiber (CCF) surface. Subsequently, filaments were prepared using modified CF and polylactic acid (PLA) and samples were obtained by FDM process. The CF-PEI-GO/PLA composites exhibited remarkable enhancements in mechanical performance relative to neat CF/PLA. Specifically, their interfacial shear strength (IFSS) doubled (101.64% increase), while impact resistance surged by 74.50%. Additionally, tensile strength and flexural strength showed substantial improvements of 31.17% and 24.86%, respectively. The interfacial enhancement mechanism was analyzed through a variety of characterization techniques such as SEM, AFM, XPS and Raman. This research offered new insights and methods towards the development of engineering materials for high performance 3d printing.
研究领域:
材料科学
·
复合材料
·
3D打印技术
·
高分子科学
·
表面工程
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Yanli Zheng,Xiaoyu Jia,Zhiyong Zhao,Yalin Ran,Meijun Du,Haiyu Ji,Yanfang Pan,Ziqin Li,Xiaowei Ma,Yue Liu,Lihua Duan,Xihong Li
Poly (butylene adipate- co -terephthalate) (PBAT)/polylactic acid (PLA) blended with compatibilizers (polycaprolactone, PCL; poly (ethylene glycol), PEG; titanium dioxide, nano-TiO 2 ) ( [email protected] composites) were developed by melt processing. Natamycin incorporated into [email protected] blend composites formed [email protected] films, which exhibited high tensile strength (24.1–43.5 MPa) and elongation at break (85.8–258.2 %), and exhibited good oxygen permeability, water vapor permeability, surface hydrophobicity and biodegradability. The in vitro results revealed that inhibition of Penicillium expansum cell growth of the [email protected] films with addition of 1.0 wt% natamycin reached 95.72 %. The [email protected] film with natamycin effectively reduced incidence of decay (1.52 %) on grapes, maintained their quality, and inhibited the growth of pathogenic fungi to up to 0.42 log cfu·g −1 . This study generates new insights into the preservation properties of antimicrobial [email protected] film, which endow it with great application potential as a novel and eco-friendly packaging material for the food industry.
研究领域:
材料科学
·
食品科学
·
生物技术
·
包装技术
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产品关联
DOI
PubMed
高影响因子
MOLECULAR PHARMACEUTICS
·
2017
·
IF 4.44
Qian Wang,Chan Li,Tianyang Ren,Shizhu Chen,Xiaoxia Ye,Hongbo Guo,Haibing He,Yu Zhang,Tian Yin,Xing-Jie Liang,Xing Tang
Bioadhesive nanoparticles based on poly(vinyl methyl ether/maleic anhydride) (PVMMA) and poly(ethylene glycol) methyl ether-b-poly(d,l-lactic acid) (mPEG-b-PLA) were produced by the emulsification solvent evaporation method. Paclitaxel was utilized as the model drug, with an encapsulation efficiency of up to 90.2 ± 4.0%. The nanoparticles were uniform and spherical in shape and exhibited a sustained drug release compared with Taxol. m-NPs also exhibited favorable bioadhesive efficiency at the same time. Coumarin 6 or DiR-loaded nanoparticles with/without PVMMA (C6-m-NPs/DiR-m-NPs or C6-p-NPs/DiR-p-NPs) were used for cellular uptake and intestinal adhesion experiments, respectively. C6-m-NPs were shown to enhance cellular uptake, and caveolae/lipid raft mediated endocytosis was the primary route for the uptake of the nanoparticles. Favorable bioadhesive efficiency led to prolonged retention in the intestine reflected by the fluorescence in isolated intestines ex vivo. In a ligated intestinal loops model, C6-m-NPs showed a clear advantage for transporting NPs across the mucus layer over C6-p-NPs and free C6. The apparent permeability coefficient (Papp) of PTX-m-NPs through Caco-2/HT29 monolayers was 1.3- and 1.6-fold higher than PTX-p-NPs and Taxol, respectively, which was consistent with the AUC0–t of different PTX formulations after oral administration in rats. PTX-m-NPs also exhibited a more effective anticancer efficacy, with an IC50 of 0.2 ± 1.4 μg/mL for A549 cell lines, further demonstrating the advantage of bioadhesive nanoparticles. The bioadhesive nanoparticles m-NPs demonstrated both mucus permeation and epithelial absorption, and thus, this bioadhesive drug delivery system has the potential to improve the bioavailability of drugs that are insoluble in the gastrointestinal environment.
研究领域:
药学
·
纳米技术
·
药物递送系统
·
癌症研究
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2019
·Vol. 167
·期 7
·070504
·
IF 3.12
Muhammad Zaheer,Hongli Xu,Bingbing Wang,Lianwei Li,Yonghong Deng
High ionic conductivity is a prerequisite for the application of solid-state polymer electrolyte towards the safe and high energy density electrochemical devices. Here we report the preparation and properties of an in-situ polymerized comb-like copolymer-based SPE (PLA/PEG-SPE) with high ionic conductivity from methyl acrylate functionalized poly(D,L-Lactide) (PLAMA) and poly(ethylene glycol) methyl ether methacrylate (PEGMA). A remarkably high ionic conductivity value of 6.9 × 10−5 S cm−1 at ambient temperature and a maximum ionic conductivity of 4.3 × 10−4 S cm−1 at 60°C were detected, with an activation energy of 0.2 eV and a Li+ transference number (tLi+) of 0.36. The PLA/PEG-SPE exhibits a wide electrochemical stability window up to 4.6 vs. Li/Li+ and very good lithium metal electrode compatibility. Solid-state LiFePO4/SPE/Li cells with integrated cathode and lithium metal deliver superior cycling stability with high discharge capacities (149 mAh g−1 as the initial specific capacity) and high capacity retention (exceeded 82% of its initial specific capacity) at 0.2 C at 60°C. The solid-state cells are also capable of being cycled at room temperature at 0.2 C. This work highlights a facile, in-situ fabrication strategy involving a vinyl-functionalized PLA precursor that yields a high-performance ion-conducting membrane attractive for lithium metal battery applications.
研究领域:
材料科学
·
电化学
·
能源存储
·
聚合物化学
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DOI
高影响因子
危险品化学品经营许可证（带存储）