Journal of Materials Chemistry B
·
2020
·Vol. 8
·期 46
·10662-10669
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IF 5.344
Sixuan Wu,Caihong Wang,Jinhong Wang,Hongliang Tan
Coordination polymers (CPs) have been extensively investigated for a variety of applications because of their tunable structures and properties. In this work, we demonstrated the potential of catalytic CPs in the fabrication of an integrated multifunctional composite for establishing a cascade amplified immunoassay. For this purpose, an Fe(III)-based CP (FeCP) with peroxidase-like activity was employed as a model of catalytic CPs to simultaneously integrate glucose oxidase (GOx) and the anti-prostate specific antigen (anti-PSA) antibody through a self-adaptive inclusion process. This leads to the formation of a dual-functional anti-PSA/GOx@FeCP composite with cascade catalytic activity and capture ability to target the antigen. Benefiting from the shielding effect of FeCPs as a host, a significantly improved stability against harsh environments can be achieved for the loaded GOx and anti-PSA antibody in the composite. On this basis, by utilizing anti-PSA/GOx@FeCPs as a detection antibody, a colorimetric immunoassay based on the cascade catalysis of GOx and FeCPs as a signal amplified enhancer was developed for the detection of PSA. Under optimal conditions, satisfactory detection results have been achieved in both buffered aqueous solutions and serum samples. We believe that this study will open up a new avenue for the rational design and fabrication of multifunctional composites while offering a new cascade amplification strategy for PSA detection.
研究领域:
生物化学
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分析化学
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材料科学
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免疫分析技术
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2023
·
IF 4.8
Tao Hu,Zeyang Bai,Di Wang,Ying Bai,Xiao Li,Zhonghua Ni
Prostatic cancer threatens the lives and health of numerous male patients all over the world. As a single-chain glycoprotein, prostate specific antigen (PSA) can be used to perform prostate cancer screening. At present, there are still problems such as time-consuming and low sensitivity in hospital detection. At the same time, electrochemical immunosensor technology has received a lot of attention due to its benefits, including high sensitivity, good selectivity, low usage and cost, and so on. Electrocatalysts are typically used to build electrochemical immunosensors for signal amplification in the recent years to realize sensitive detection of cancer biomarkers such as PSA. In recent years, three-dimensional nanomaterials have been widely used in the study of electrocatalysts, but the study of the sensitivity for PSA detection is still very scarce. The present work reported the application of a 3D graphene aerogel(GA)/AuNPs/Nafion electrochemical platform for highly sensitive PSA quantification. The electrochemical immunosensor has been designed by immobilizing aptamers on the screen-printed electrode (SPE) surface modified by GA/AuNPs/Nafion. Scanning electron microscopy (SEM), Raman spectra, and electrochemical characterization demonstrated the successful combination of GA/AuNPs/Nafion/SPE. Based on electrochemical impedance spectroscopy (EIS), the proposed immunosensor has demonstrated excellent electrochemical performance, exhibiting sensitive and stable responses for electrochemical detection of PSA ranging from 0.05 to 50 ng∙mL −1 with a limit of detection (LOD) of 0.0306 ng∙mL −1 . This study has great potential application in PSA detection and provides a valuable reference for point-of-care testing (POCT) of prostate cancer.
研究领域:
生物化学
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分析化学
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生物传感器
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癌症研究
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材料科学
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电化学
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产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Ning Xia,Dehua Deng,Suling Yang,Yuanqiang Hao,Luyan Wang,Yuwei Liu,Cuixia An,Qiuyang Han,Lin Liu
This work reported that protease can be used as the signal label to amplify the signal of electrochemical immunosensors. Carbon nanotube (CNT) was used as the carrier of recognition antibody and signal label. The nanolabel was captured by sensor electrode via a sandwich-type immunoreaction. Protease on the surface of nanolabel can catalyze the hydrolysis of peptide substrate to liberate an amino terminal Cu(II)- and Ni(II)-binding (ATCUN) peptide. The released ATCUN peptide can bind with Cu(II) to generate an electrocatalyst which exhibits high electrocatalytic ability for water oxidation. Thus, the signal was amplified again by the redox cycling between ATCUN-Cu(II) and H 2 O. The immunosensor exhibits low background current because of the poor redox activity of peptide substrate and the high oxidation overpotential of H 2 O molecule. Based on the signal amplification of protease and redox cycling, the target concentration as low as 10 pg/mL can be readily measured with prostate-specific antigen (PSA) as the model analyte. The proposed immunosensor was applied to determine PSA in human serum samples with satisfactory results.
研究领域:
分析化学
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生物化学
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纳米技术
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电化学
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免疫学
✓ 已验证
DOI
高影响因子
ANALYTICAL CHEMISTRY
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2022
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IF 8.008
Xiaolin Yang,Yuxi Wei,Zimei Wang,Junxia Wang,Honglan Qi,Qiang Gao,Chengxiao Zhang
This work reports a highly efficient electrogenerated chemiluminescence (ECL) quenching on lipid-coated multifunctional magnetic nanoparticles (MMNP) for the determination of proteases incorporating membrane-confined quenching with a specific cleavage reaction for the first time. A new ruthenium complex [Ru(bpy)2(ddcbpy)](PF6)2 (bpy = 2,2′-bipyridine, ddcbpy = 4,4′-didodecyl-carbonyl-2,2′-bipyridine with two hydrophobic long alkyl chains) was synthesized as a signal probe, while [cholesterol-(CH2)6-HSSKLQK(peptide)-ferrocene (quencher)] was designed as a specific peptide-quencher probe. The MMNP were prepared by inserting both the signal probe and the peptide-quencher probe into the cholesterol–phospholipid-coated Fe3O4 magnetic nanoparticles (Fe3O4 NP, ∼200 nm). When prostate specific antigen (PSA) taken as a model analyte was introduced into the suspension of MMNP, PSA cleaved the amide bond of SK in cholesterol-(CH2)6-HSSKLQK-Fc, and then the cleaved peptide-motif-Fc-quencher was deviated from the MMNP, resulting in the increase in the ECL intensity. It was found that the ECL quenching constant of [Ru(bpy)2(ddcbpy)]2+ on MMNP (KSV, NP/lipECL =2.68 × 107 M–1) is 137-folds higher than that on the lipid-coated electrode (KSV, lipECL=1.95 × 105 M–1) and 391-folds higher than that in the solution (KSV, aqECL =6.86 × 104 M–1). The ECL emission of Ru(bpy)32+ derivative-attached Fe3O4 NP was observed at ∼1.2 V, involving the tunnel-electron transfer pathway (TPA+ Ru(bpy)33+ = Ru(bpy)32+*). Based on the highly efficient ECL quenching of the ruthenium complex by ferrocene on the MMNP, a new ECL method was developed for PSA with a linear range from 0.01 to 1.0 ng/mL and a limit of detection of 3.0 pg/mL. This work demonstrates that the approach of ECL quenching by ferrocene on lipid-coated Fe3O4 NP is promising and could be easily extended to determine other proteases.
研究领域:
分析化学
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生物化学
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纳米技术
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分子生物学
✓ 已验证
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2024
·
IF 5.7
Ning Xia,Fengli Gao,Gang Liu,Yong Chang,Lin Liu
Background Small molecule mimics offer the advantages of easy preparation, good thermodynamic stability, and reproducible catalytic activity. However, most of the reported organic artificial mimics face challenges including low catalytic activity, oxidative self-destruction, and auto-aggregation into inactive dimers. Therefore, novel organic mimics with high catalytic activity as well as good thermal and environmental stability are highly desirable. Results We found that pyrroloquinoline quinone (PQQ), a nutritionally important growth factor, displayed significant peroxidase-like activity to catalyze the oxidation of colorless 3,3′,5,5′-tetramethylbenzidine (TMB) into blue oxidized TMB (oxTMB) by H 2 O 2 . Its catalytic activity surpassed that of other organic small molecules reported previously, including fluorescein derivatives and adenosine/guanosine triphosphate. Based on the reduction of oxTMB by the enzymatically generated ascorbic acid (AA), the PQQ/TMB-based system was used to monitor alkaline phosphatase (ALP) activity with ascorbic acid 2−phosphate (AAP) as the substrate. This sensing system allowed for the determination of ALP with a detection limit of 1 mU/mL. Furthermore, we discovered that PQQ can coordinate with Cu 2+ to form metal-organic nanoparticles. The building blocks of two active cofactors endowed the nanoparticles with impressive functionalities for biocatalytic applications. With Cu-PQQ nanoparticles as signal labels, colorimetric immunoassays of prostate specific antigen (PSA) were achieved with a lowest detectable concentration down to 0.1 pg/mL. Significance This work is valuable for the design of novel biosensors by utilizing PQQ as an artificial enzyme because of its high catalytic activity, good stability, ease of storage, and simple chemical structure.
研究领域:
生物化学
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分析化学
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纳米技术
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生物传感器
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产品关联
DOI
PubMed
高影响因子
Nanoscale
·
2018
·Vol. 10
·期 25
·11942-11947
·
IF 7.233
Lin Yang,Shu Jun Zhen,Yuan Fang Li,Cheng Zhi Huang
Graphene oxide (GO) exhibits distinctive Raman scattering features for its high frequency D (disordered) and tangential modes (G-band), which are characteristically sharp at 1580 cm−1 and 1350 cm−1, respectively, but are too weak for sensitive quantitation purposes. By depositing silver nanoparticles on the surface of GO in this contribution, both D and G bands of GO become enhanced. The enzyme label of this method controls the dissolution of silver nanoparticles on the surface of GO through hydrogen peroxide which is produced by the oxidation of the enzyme substrate. With the dissolution of the silver nanoparticles a greatly decreased SERS signal of GO was obtained. This strategy involves dual signal amplification of the enzyme and nanocomposites to improve the detection sensitivity. As a proof of concept, prostate specific antigen (PSA), a biomarker for prostate cancer, is successfully detected as a target by forming a sandwich structure in immunoassay. The SERS immunoassay possesses excellent analytical performance in the range 0.5 pg mL−1 to 500 pg mL−1 with a limit of detection of 0.23 pg mL−1, making the detection of PSA serum samples from prostate cancer patients satisfactory, demonstrating that the sensitive enzyme-assisted dissolved AgNPs SERS immunoassay of PSA has potential applications in clinical diagnosis.
研究领域:
纳米技术
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生物医学工程
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分析化学
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癌症研究
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材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2021
·
IF 6.558
Biying Zhao,Ping Miao,Ziheng Hu,Xinying Zhang,Xue Geng,Yingying Chen,Lingyan Feng
Prostate specific antigen (PSA) has become a potential biomarker for detecting prostate cancer (PCa) in the early stage. Herein, we report a target-induced resolution for the detection of PSA sensitively and specifically by amperometric electrochemical measurements. To meet a satisfactory performance, three conformations of pre-design DNA aptamers including two stem-loop structures and a double strand structure have been investigated and compared. All of them are immobilized on gold electrode as capture probes with redox-active molecular. The mechanism of signal transduction depends on molecular recognition events involving aptamer conformational changes, thus influencing the charge transfer. A short, single-stranded DNA (ssDNA) pseudoknot forming two stem-loop structural aptamers with labeled MB at the 3′ -terminus was found to posse the highest signal variation than other structure when induced by PSA due to the strong conformational change. With the optimized capture strand, the aptasensor showed the peak current increase of MB by the binding relationship between PSA and the sensor over a wide concentration range of 4 magnitude orders. The proposed aptasensor exhibited a wide detection range from 10 pg/mL to 500 ng/mL with a low detection limit of 1.24 pg/mL (S/N = 3). Moreover, the electrochemical aptasensor demonstrated good reproducibility, sensitivity, selectivity , and reliability for the detection of PSA. We also found the aptasensor had a good response in the human serum samples, making this device easy to operate for the detection of the PSA physiological concentration.
研究领域:
生物医学工程
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分析化学
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癌症诊断
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生物传感器
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Nano Materials
·
2022
·
IF 6.14
Yuting Gao,Yan Wu,Pengcheng Huang,Fang-Ying Wu
The enzyme-linked immunosorbent assay (ELISA) commonly used for the detection of cancer biomarkers suffers from low sensitivity. Although emerging plasmonic ELISA has become a candidate owing to unprecedented sensitivity and operation convenience, the single-mode colorimetric readout mainly dependent on the monodisperse or aggregated state of gold (Au) or silver (Ag) nanoparticles remains limited in clinical applications because of poor accuracy from uncertain experimental and environmental factors. Herein, we presented a carbon dot (CD)-encapsulated plasmonic core-satellite single nanoprobe, CDs/Ag–Au, prepared by a simple sequential in situ reduction strategy without needing additional reducing agents. The developed nanoprobe could simultaneously generate notable colorimetric and fluorometric signals triggered by H2O2 due to its selective etching to the Ag core accompanied by the release of CDs. Inspired by this dual responsiveness, we further utilized this integrated nanoprobe as the signal reporter in the glucose oxidase-involved immunoassay for prostate-specific antigen (PSA), a model cancer biomarker. Both the clear changes in the localized surface plasmon resonance peak and remarkable fluorescence enhancement were closely correlated with the target concentration, thus achieving highly sensitive and reliable dual-mode detection of PSA in a simple manner. This approach also enabled dual-mode visual detection and evaluation of the PSA level in human serum samples.
研究领域:
纳米技术
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生物传感器
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癌症诊断
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分析化学
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2016
·
IF 7.145
Jinjin Guo,Junchun Wang,Junqing Zhao,Zilin Guo,Yuzhong Zhang
In this work, a novel electrochemical immunoassay protocol has been reported for simultaneous determination of multiple tumor biomarkers based on DNA hybridization chain reaction (HCR) for signal amplification. Alpha-fetoprotein (AFP) and prostate specific antigen (PSA) were selected as model biomarkers. The immunoassay protocol contained primary antibodies immobilized on gold nanoparticles (Au NPs), secondary antibodies conjugated with DNA concatemer from HCR of primer, auxiliary probe, and signal probe labeled with signal molecules (methyleneblue (MB) and ferrocene (Fc)). In the presence of target biomarkers, the sandwich immunocomplex was formed between the primary antibodies and secondary antibodies bioconjugates carrying numerous signal molecules. As a result, two well-resolved reduction peaks, one was at −0.35 V (corresponding to MB) and other was at 0.33 V (corresponding to Fc; both vs SCE), were obtained in differential pulse voltammetry, and peak currents changed were related to the level of biomarkers. Under optimal conditions, the electrochemical immunoassay exhibited a wide linear response range (0.5 pg mL–1 to 50 ng mL–1) and low detection limits (PSA, 0.17 pg mL–1; AFP, 0.25 pg mL–1) (at S/N = 3). In addition, the immunoassay was evaluated by analyzing simulate human serum sample, and the recoveries obtained were within 99.4–107.6% for PSA and 97.9–108.2% for AFP, indicating the immnuoassay could be applied to the simultaneous detection of AFP and PSA in human serum samples.
研究领域:
生物化学
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分析化学
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纳米技术
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癌症研究
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生物医学工程
✓ 已验证
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2020
·
IF 5.977
Yingcong Zhang,Ze Zhang,Shengzhong Rong,Hongwei Yu,Hongmin Gao,Qian Sha,Ping Ding,Hongzhi Pan,Dong Chang
An ultrasensitive sandwich-type electrochemiluminescence (ECL) immunosensor was developed for detection of prostate specific antigen (PSA) using an amplification strategy based on ZnO nanorods- l -cysteine-luminol nanocomposites and the biotin-streptavidin system. The biotin-streptavidin system served as a capture probe to increase the number of antibodies. ZnO nanorods not only acted as a nanocarrier that increased the number of luminol molecules and secondary antibodies, but also enhanced the ECL signal of luminol-H 2 O 2 system by promoting H 2 O 2 decomposition, which can further increase ECL intensity. Under optimized conditions, the proposed immunosensor demonstrated excellent analytical performance with a wide linear detection range of 0.03 pg mL −1 to 30 ng mL −1 and a detection limit of 0.01 pg mL −1 (the detection limit in real samples was 0.021 pg mL −1 ). This method exhibited excellent stability, reproducibility, and selectivity. In addition, the results of PSA determinations in human serum samples obtained using the proposed immunosensor were consistent with data collected using the commercial chemiluminescence immunoassay analyzer.
研究领域:
生物化学
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分析化学
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生物医学工程
·
癌症诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2017
·
IF 4.95
Ziyu Han,Yanyan Wang,Xuexin Duan
This paper reports a surface functionalization strategy for protein detections based on biotin-derivatized poly( l -lysine)-grafted oligo-ethylene glycol (PLL- g -OEG x -Biotin) copolymers . Such strategy can be used to attach the biomolecule receptors in a reproducible way simply by incubation of the transducer element in a solution containing such copolymers which largely facilitated the sensor functionalization at an industrial scale. As the synthesized copolymers are cationic in physiology pH, surface biotinylation can be easily achieved via electrostatic adsorption on negatively charged sensor surface. Biotinylated receptors can be subsequently attached through well-defined biotin-streptavidin interaction. In this work, the bioactive sensor surfaces were applied for mouse IgG and prostate specific antigen (PSA) detections using quartz crystal microbalance (QCM), optical sensor (BioLayer Interferometry) and conventional ELISA test (colorimetry). A limit of detection (LOD) of 0.5 nM was achieved for PSA detections both in HEPES buffer and serum dilutions in ELISA tests. The synthesized PLL- g -OEG x -Biotin copolymers with different OEG chain length were also compared for their biosensing performance. Moreover, the surface regeneration was achieved by pH stimulation to remove the copolymers and the bonded analytes , while maintaining the sensor reusability as well. Thus, the developed PLL- g -OEG x -Biotin surface assembling strategy is believed to be a versatile surface coating method for protein detections with multi-sensor compatibility.
研究领域:
生物技术
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生物传感器
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分析化学
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材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2017
·
IF 7.78
Yu-Ling Wang,Fu-Rao Liu,Jun-Tao Cao,Shu-Wei Ren,Yan-Ming Liu
The identification of tumor markers is of great importance for clinical diagnosis but accurate detection with high sensitivity is still a great challenge. In present work, a spatial-resolved dual-signal-output electrochemiluminescent (ECL) ratiometric assay platform was constructed for sensitive detection of prostate specific antigen (PSA) on a dual-disk glassy carbon electrode. To fabricate the platform, flower-like CdS three-dimensional (3D) assemblies and Ru(bpy) 3 2+ -conjugated silica nanoparticles (Ru(bpy) 3 2+ @RuSi NPs), were immobilized onto the two disks as cathodic and anodic ECL emitters, respectively. After the stepwise modification of the gold nanoparticles , antibody for PSA, and bovine serum albumin onto the two disks respectively, the Ru(bpy) 3 2+ @RuSi NPs-based disk were incubated with varied concentration of PSA as working electrode , whereas the flower-like CdS 3D assemblies-based disk with fixed concentration of PSA were taken as internal reference electrode . The label free assay of PSA was realized by the ratio of anodic ECL signal from working electrode to the cathodic ECL signal from the internal reference electrode (ECL anode /ECL cathode ). On the basis of the spatial-resolved dual-signal-output ratiometric ECL sensor, the PSA can be detected accurately with a linear range of 0.001 – 50 ng/mL at a concentration as low as 0.34 pg/mL. Furthermore, the proposed method was applied for PSA determination in human serum samples with satisfying results. Thanks to the same modified process of the two disks, this universal design well avoids environmental errors including the interference caused in the biological recognition process, which effectively reduces the false positive or negative errors, exhibiting a greatly improved accuracy, reliability and sensitivity.
研究领域:
分析化学
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生物化学
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生物医学工程
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临床诊断
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纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）