Artificial Cells Nanomedicine and Biotechnology
·
2020
·
IF 3.343
Baiji Xue,Jinxiang Huang,Haoxiang Zhang,Bingliang Li,Man Xu,Yuelan Zhang,Maobin Xie,Xuefeng Li
Curcumin (CM) is a natural polyphenolic compound with multiple biomedical functions. However, clinical applications face more challenges due to its low dissolution rate and poor bioavailability. Micronization is an effective strategy to overcome these drawbacks. Herein, CM nanoparticles (CM NPs, ∼300 nm) were fabricated using solution enhanced dispersion by supercritical CO2 (SEDS). The solubility of CM NPs was remarkably enhanced. Aim to study the effects of micronization on the biological functions of CM, we investigated the antibacterial activity of original CM and CM NPs upon Pseudomonas aeruginosa. In vitro, the minimal inhibitory concentrations (MIC) assay, solid-medium spot assay, growth kinetics assay and morphologic observation using atomic force microscopy (AFM) confirmed that the anti-P. aeruginosa activity of CM NPs was enhanced compared to original CM. Moreover, CM NPs also showed stronger inhibition for adhesion and biofilm formation of P. aeruginosa compared to original CM. Experiments on mice infected with P. aeruginosa showed that CM NPs have a better therapeutic effect than the original CM in vivo. In summary, CM NPs may be a novel and promising therapeutic candidate for bacterial infection.
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International Journal of Electrochemical Science
·
2023
·
IF 1.5
Qinghong Li,Lihua Dong,Yi Yang,Zeqi Wu,Hongling Zhu,Yaohua Dong,Yuanyuan Shen,Li Zhang,Qingye Lu
Microbial contamination of aqueous metalworking fluids (MWFs) is of significant concern in metal process industries. In this study, a dominant Pseudomonas sp. strain ( P. xiamenensis ) was isolated from waste metalworking fluids (WMWFs) and the biodegradation of model MWF was studied under the influence of P. xiamenensis. Results showed that nitric additives in MWF were severely degraded by bacteria, leading to a significant increase in the turbidity of the MWF. The occurrence of microbially influenced corrosion of AISI 1045 carbon steel in MWF containing P. xiamenensis , was proven by morphological analysis and electrochemical characterization. The corrosion rate of the metal in MWF in the presence of bacteria was three-fold greater than in the absence of bacteria. Electrochemical results and surface analysis elucidated that the additive film was decomposed by P. xaimenensis adhered to the steel surface and a biofilm, which was controlled by diffusion replacing the additive film. The biofilm was reinforced by the reaction of metabolites and Ca 2+ and seriously localized corrosion behavior was observed in carbon steel immersed in the bio-contaminated MWF due to the microbial influence.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2021
·
IF 5.279
Wei Song,Kai Zheng,Xin Xu,Cong Gao,Liang Guo,Jia Liu,Xiulai Chen,Liming Liu,Guipeng Hu,Jing Wu
l-Ascorbic acid-2-phosphate (AsA-2P) is stable in aqueous solution and at high temperatures and is widely used in foods, pharmaceuticals, cosmetics, and fodders; however, practical application of enzymatic synthesis methods to promote industrial-scale production of AsA-2P remains a major challenge. In this study, we enhanced the phosphorylation efficiency of Pseudomonas aeruginosa acid phosphatase (APase; EC 3.1.3.2) for AsA-2P production via protein engineering. Among the mutants obtained, we selected the most efficient mutant (Var5; G125E/D135T/S136N), which exhibited an increased kcat of 18.6 s–1 and a Km for AsA of 223.9 mM. In addition, Var5 exhibited a maximum enzyme activity of 2080.4 U/L after 10 h of fermentation, which was 80% higher than the wild-type enzyme. Furthermore, under optimal conditions, Var5 showed a maximal conversion of 48.6% and achieved a final AsA-2P titer of 61.5 g/L at 8 h, which is considerably higher than that reported for other similar biocatalytic approaches. These findings demonstrate the potential of this method for the large-scale production of AsA-2P.
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CHEMICAL PAPERS
·
2020
·Vol. 75
·期 3
·1069-1081
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IF 1.68
Arukalam Innocent O.,Xu Dake,Li Ying
Pseudomonas aeruginosa ( P. aeruginosa ) has been implicated in the attachment and formation of marine biofilms which initiate biofouling and biocorrosion. To this end, several hydrophobic and superhydrophobic coatings have been suggested for anti-bacterial application, but some possess toxic substances which affect useful aquatic lives. Thus, the aim of this study was to appraise the anti-bacterial and anti-corrosion performance of hydrophobic perfluorodecyltrichlorosilane (FDTS)-modified poly (dimethylsiloxane) (PDMS)-ZnO coating using P. aeruginosa . The surface analytical, physico-chemical and electrochemical techniques were used to investigate the properties of the coatings. Results show that FDTS-modified PDMS-ZnO coating displayed higher resistance to adhesion of P. aeruginosa biofilm and better corrosion resistance performance than the unmodified coating. The outstanding performance of FDTS-modified coating was attributed to the low surface activity of FDTS which increased the kinetic barrier between the coating surface and biofilm. It is therefore anticipated that these results would provide insight in the design of future anti-bacterial coatings for marine application.
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CHEMICAL ENGINEERING JOURNAL
·
2026
·
IF 12.5
Jieyi Pan,Jiaying Zhao,Leijie Tian,Yingying Sun,Pengchao Fang,Yili Chen,Haiyan Hu,Shihui Yu
An inhalable core–shell nanoplatform is designed with pH-responsive release for dual modulation of host and pathogen. • A mucolytic polyglutamic acid shell enables efficient penetration of patient-derived mucus and biofilm disruption. • The polymer-based core delivers combinatorial antifibrotic and antibacterial agents in the acidic infection microenvironment. • The engineered nanocarrier eradicates resistant P. aeruginosa (>99%) and haltes infection-driven pulmonary fibrosis in vivo. Chronic pulmonary Pseudomonas aeruginosa ( P. aeruginosa ) infection in immunocompromised patients establishes a stubborn pathological triad of thickened mucus, dense biofilm, and excessive inflammation, driving pulmonary fibrosis and irreversible lung function decline. Current therapies, reliant on intensified antibiotics or nebulized polymyxin, fail to thoroughly disintegrate this triad, address the fibrotic drive, and exacerbate drug resistance. To address these challenges, we engineered inhalable nanoparticles (CPAP/PRIP NPs) with a layered structure, formulated using two custom-synthesized polymers. The pH-responsive core is self-assembled from a bactericidal and antioxidant/anti-inflammatory polymer (all-trans-retinal-ε-poly- l -lysine), which encapsulates the ion pairs composed of bactericidal polymyxin B (PB) and anti-fibrotic rhein. This ion-pairing strategy significantly enhanced the drug-loading efficiency of PB, while the pH-responsive design enables localized drug release specifically at infection sites (pH 5.5 to 6.5). To endow mucus-dissolving and penetrating capabilities, the core is further coated with a mucolytic/hydrophilic cysteamine-polyglutamic acid polymer shell. Nebulized CPAP/PRIP NPs exerted two key therapeutic actions: (i) efficiently eradicated difficult-to-treat-resistant P. aeruginosa (DTR P. aeruginosa ) via high drug loading, mucus dissolution and penetration, biofilm permeability, bactericidal activity, and virulence suppression; (ii) attenuated infection-driven pulmonary fibrosis via transforming growth factor β1 suppression and anti-inflammation/antioxidant effects. In vitro, CPAP/PRIP NPs disrupted the construction of patient-derived mucus, reduced its viscosity, and attenuated TGF-β1-driven pulmonary fibroblast invasiveness and migration. In vivo, CPAP/PRIP NPs ameliorated mucus layer, normalized cytokines, achieved over 99% eradication of DTR P. aeruginosa , and halted infection-driven pulmonary fibrosis. This integrated strategy represents an innovative approach for simultaneously eradicating DTR P. aeruginosa infection & halting infection-driven pulmonary fibrosis.
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JOURNAL OF NANOPARTICLE RESEARCH
·
2026
·Vol. 28
·期 9
·241
·
IF 3.5
Li E.,Huang Qin,Zhan Jidong
Complicated urinary tract infections (UTIs) caused by Pseudomonas aeruginosa are difficult to treat due to biofilm-mediated resistance and the limitations of conventional antibiotics like ceftazidime (CAZ). To address this challenge, a nanotherapeutic system, ZIF-8@CAZ, was developed by encapsulating CAZ within a zeolitic imidazolate framework-8 (ZIF-8) nanocarrier. This nanocomposite exhibited sustained drug release and significantly enhanced in vitro antibacterial efficacy against P. aeruginosa PAO1, as evidenced by a substantially lower minimum inhibitory concentration compared to free CAZ, along with potent anti-biofilm activity. In a murine model of ascending UTI, ZIF-8@CAZ treatment resulted in a dramatic reduction of bacterial burden in bladder tissues, significant attenuation of inflammation (as shown by decreased levels of IL-6 and TNF-α), and superior amelioration of tissue pathology compared to an equivalent dose of free CAZ. Furthermore, comprehensive biosafety evaluations confirmed the excellent biocompatibility of ZIF-8@CAZ, with no observed systemic toxicity. These findings demonstrate that ZIF-8@CAZ is a promising nanoplatform for the effective treatment of biofilm-associated UTIs.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Chun Zhang,Kunhong Zhao,Zilin Wu,Na Tao,Weijia Yang,Zhaojia Li,Moxian Chen,Xiangyang Li
Bacterial canker caused by Pseudomonas syringae pv. actinidiae (Psa) severely impacts global kiwifruit production. This study shows that 5-fluoroindole exerts significant bactericidal activity against Psa, with a half-maximal effective concentration (EC50) of 15.34 μg/mL, demonstrating a higher efficacy than the positive control copper hydroxide (EC50 = 58.65 μg/mL). 5-Fluoroindole disrupts membrane integrity, induces oxygen species (ROS) accumulation, and triggers apoptosis in Psa. Transcriptome analysis reveals that 5-fluoroindole treatment increased the expression of the methionine synthase II (MetE) gene by 6.28 fold compared with the expression of this gene in the control (CK) group. Microscale thermophoresis and isothermal titration calorimetry show that the dissociation constants of the binding of 5-fluoroindole to MetE protein are 0.33 and 8.55 μM, respectively. Molecular docking experiments indicate that Asp693 is a key binding site and that there is no specific binding between 5-fluoroindole and the Psa MetED693A mutant. This study provides valuable insights for developing effective agents to control kiwifruit bacterial canker.
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ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2022
·Vol. 30
·期 10
·28247-28258
·
IF 5.19
Shi Guangyu,Hu Jiayuan,Cheng Yuanyuan,Shi Weilin,Chen Yan
The effect of Pseudomonas aeruginosa ( P. aeruginosa ) on the phytoremediation efficiency of ryegrass on soil contaminated with nonylphenol (NP) and cadmium (Cd) was investigated by pot experiments. Pseudomonas aeruginosa application stimulated the adsorption of Cd by ryegrass and facilitated the biodegradation of NP in the soil. Exogenous P. aeruginosa inoculation increased the activities of urease, dehydrogenase, and polyphenol oxidase in the soil of the T4 treatment by 38.5%, 50.0%, and 56.5% compared to that of the T2 treatment, respectively. There was a significant positive correlation between the activities of dehydrogenase and polyphenol oxidase and the NP removal rate ( P < 0.001). The relative abundances of beneficial microorganisms (such as Sphingomonas , Lysobacter , Streptomyces , Chloroflexia, Deltaproteobacteria, and Alphaproteobacteria) were increased as a result of P. aeruginosa inoculation. These microorganisms play important roles in nutrient cycling, Cd adsorption, and NP degradation. Additionally, P. aeruginosa was not the dominate bacterial species at the end of the experiment. According to this study, P. aeruginosa application improved the phytoremediation efficiency of ryegrass on soil contaminated with NP and Cd, with a minimal risk of alien microbial invasion.
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BIOCHEMICAL ENGINEERING JOURNAL
·
2021
·
IF 3.978
Jingjing Zhang,Feng Qu,Taobo Liang,Sidi Lv,Wen Li,Qixiu You,Bolu Chen,Hengyi Xu
Pseudomonas aeruginosa is one of the most commonly isolated nosocomial pathogens and is responsible for approximately 10 % of all hospital-acquired infections. P. aeruginosa is difficult to treat with known antibiotics due to its increasing antibiotic resistance. In our study, Ag NPs coated with ε-polylysine (ε-PL) and polyethyleneimine (PEI) were synthesized. These materials showed strong anti- P. aeruginosa capacity at an low concentration (IC50: 7.284 μg/mL). In addition, three drug-resistant clinical strains and biofilm formation were inhibited by ε-PL/PEI-coated Ag NPs (MBIC: 15.63 μg/mL). We further studied the antibacterial mechanism of Ag NPs on different levels and found that a) the amount of reactive oxygen species (ROS) increased, b) the expression of oxidative stress-related genes were up-regulated, c) the activity of total superoxide dismutase decreased, and d) the inhibitory rate is increased on P. aeruginosa cells. These antibacterial effects were dependent on an increasing concentration of Ag NPs but were neutralized by the ROS scavenger N-acetyl-L-cysteine. Therefore, we suggested that oxidative stress induced by ROS is the primary antibacterial mechanism of synthesized Ag NPs.
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SURFACE & COATINGS TECHNOLOGY
·
2020
·
IF 3.784
Xin Cheng,Jiashun Shi,Weigang Wang,Huimin Liao,Shiqiang Chen,Guangzhou Liu,Jvna Chen
Biofilm formation is an important cause of microbiologically influenced corrosion (MIC), and inhibition of microbial adhesion is one of the most effective ways to control biofilm formation. In this work, nanostructured functional film of EH40 steel is prepared by one-step anodization in NaOH solution to inhibit adhesion of Pseudomonas aeruginosa ( P. aeruginosa ) without and with light. Results show that the functional films fabricated on EH40 steel surface in a series of NaOH solutions inhibit the adhesion of P. aeruginosa , and its anti-adhesion performance first increases and then decreases with varying concentrations of NaOH solution. The optimal anti-adhesion performance under dark condition, i.e., anti-adhesion rate of 92.7% compared with bare steel, is obtained for the nanostructured oxide film fabricated in 12 M NaOH solution under anodization potential of 1.0 V for 2 h. Under visible light irradiation, the best anti-adhesion rate increases to 99.9% due to hydroxyl radicals ( OH) produced by the nanostructured oxide film with photocatalytic activity .
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JOURNAL OF HAZARDOUS MATERIALS
·
2020
·
IF 9.038
Yunli Liu,Haiyang Hu,Giulio Zanaroli,Ping Xu,Hongzhi Tang
Polycyclic aromatic hydrocarbons (PAHs) and heterocyclic derivatives are organic pollutants that pose a serious health risk to human beings. In this study, a newly isolated Pseudomonas brassicacearum strain MPDS could effectively degrade PAHs and heterocyclic derivatives, including naphthalene , fluorene, dibenzofuran (DBF) and dibenzothiophene (DBT). Notably, strain MPDS is able to degrade fluorene, DBF and DBT uniquely via a lateral dioxygenation pathway, while most reported strains degrade fluorene, DBF and DBT via an angular dioxygenation pathway or co-metabolize them via a lateral dioxygenation pathway. Strain MPDS completely degraded 50 mg naphthalene (in 50 mL medium) in 84 h, and OD 600 reached 1.0–1.1; while, it stabilized at OD 600 0.5–0.6 with 5 mg fluorene or DBF or DBT. Meanwhile, 65.7% DBF and 32.1% DBT were degraded in 96 h, and 40.3% fluorene was degraded in 72 h, respectively. Through genomic and transcriptomic analyses, and comparative genomic analysis with another DBF degradation strain, relevant gene clusters were predicted, and a naphthalene-degrading gene cluster was identified. This study provides understanding of degradation of PAHs and their heterocyclic derivatives, as well as new insights into the lateral dioxygenation pathway of relevant contaminants.
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SCIENCE OF THE TOTAL ENVIRONMENT
·
2020
·
IF 6.551
Nai-Xian Feng,Yu-Xi Feng,Qi-Feng Liang,Xin Chen,Lei Xiang,Hai-Ming Zhao,Bai-Lin Liu,Gang Cao,Yan-Wen Li,Hui Li,Quan-Ying Cai,Ce-Hui Mo,Ming Hung Wong
Phthalate acid esters (PAEs) are environmentally ubiquitous and have aroused a worldwide concern due to their threats to environment and human health. Di-n-butyl phthalate (DBP) is one of the most frequently observed PAEs in the environment. In this study, a novel bacterium identified as Pseudomonas sp. YJB6 that isolated from PAEs-contaminated soil was determined to have strong DBP-degrading activity. A complete degradation of DBP in 200 mg/L was achieved within 3 days when YJB6 was cultivated at 31.4 °C with an initial inoculation size of 0.6 (OD600) in basic mineral salts liquid medium (MSM), pH 7.6. The degradation curves of DBP (50–2000 mg/L) fitted well the first-order kinetics model, with a half-life ( t 1/2 ) ranging from 0.86 to 1.88 d. The main degradation intermediates were identified as butyl-ethyl phthalate (BEP), mono-butyl phthalate (MBP), phthalic acid (PA) and benzoic acid (BA), indicating a new complex and complete biodegradation pathway presented by YJB6. DBP might be metabolized through de-esterification, β-oxidation, and hydrolysis, followed by entering the Krebs cycle of YJB6 as a final step. Strain YJB6 was successfully immobilized with sodium alginate (SA), polyvinyl alcohol (PVA), and SA-PVA. The immobilization significantly improved the stability and adaptability of the cells thus resulting in high volumetric DBP-degrading rates compared to that of the freely suspended cells. In addition, these immobilized cells can be reused for many cycles with well conserved in DBP-degrading activity. The ideal DBP degrading ability of the free and immobilized YJB6 cells suggests that strain YJB6, especially the SA-PVA+ YJB6 promises great potential to remove hazardous PAEs.
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