查找使用阿拉丁产品的科研文献

Reduction-Responsive Nanoparticles Self-Assembled from Sericin-ss-Doxorubicin Conjugate for Hydrophobic IR780 Delivery with Integration of Antitumor Chemo-Phototherapy

Lulu Zhang,Haiyang Liu,Jin Ren,Junyi Shao,Yanrong Cao,Shuangshuang Wang,Changchun Peng,Shengwen Shao,Haixia Ge,Jingmou Yu

Background Stimuli-responsive polymer-drug nanoparticles have emerged as a versatile and effective platform for delivering drugs in the treatment of malignant tumors. The integration of chemo-phototherapy has gained increasing prominence due to its remarkable synergistic antitumor effects.Methods Hydrophilic sericin (Ser) was linked to antitumor drug doxorubicin (DOX) via a disulfide bond. The obtained Ser-ss-DOX conjugate was characterized by Fourier-transform infrared spectroscopy (FTIR), proton nuclear magnetic resonance (1H NMR) and dynamic light scattering (DLS). The near-infrared (NIR) fluorescent dye IR780 was encapsulated into Ser-ss-DOX by a dialysis method. The physical state of IR780 within Ser-ss-DOX/IR780 nanoparticles was analyzed by differential scanning calorimetry (DSC). The photothermal properties and drug release behavior of IR780-loaded nanoparticles were systematically investigated. Cellular uptake and reactive oxygen species (ROS) generation capacity and in vitro cytotoxicity of IR780-containing nanoparticles were studied in 4T1 breast cancer cells. In addition, 3D multicellular tumor spheroid model was conducted to investigate the antitumor activity of the nanoparticles.Results The Ser-ss-DOX conjugate was successfully synthesized. Ser-ss-DOX and Ser-ss-DOX/IR780 nanoparticles were spherical in shape, and their particle sizes measured by DLS were 326 and 190 nm, respectively. IR780 was in an amorphous state within the Ser-ss-DOX/IR780 nanoparticles. The loading contents of DOX and IR780 were 8.26% and 2.78%, respectively. Ser-ss-DOX/IR780 exhibited ideal photothermal properties in vitro and reduction-sensitive drug release in a high glutathione (GSH) environment. Ser-ss-DOX/IR780 displayed higher cellular uptake in 4T1 cells than Ser-ss-DOX and free DOX. Under 808 nm laser irradiation, Ser-ss-DOX/IR780 showed a strong capacity to generate ROS. Importantly, Ser-ss-DOX/IR780 with laser irradiation effectively inhibited the growth of 4T1 cells and 3D multicellular tumor spheroids.Conclusion This work demonstrated that Ser-ss-DOX/IR780 integrated dual functionalities of chemotherapy and phototherapy, thereby enabling substantial potential for application in tumor treatment.

Cascade-Targeting of Charge-Reversal and Disulfide Bonds Shielding for Efficient DOX Delivery of Multistage Sensitive MSNs-COS-SS-CMC

Lan Cui,Wentao Liu,Hao Liu,Qian Qin,Shuangxia Wu,Suqin He,Zhenya Zhang,Xinchang Pang,Chengshen Zhu

Reductive triblock copolymer micelles with a dynamic covalent linkage deliver antimiR-21 for gastric cancer therapy

Changyu He,Zhen Zhang,Qinglai Yang,Qing Chang,Zhifeng Shao,Bing Gong,Yu-Mei Shen,Bingya Liu,Zhenggang Zhu

A reductive tri-block copolymer PEG-SS-PLA-SS-PEI with a double disulphide linkage in the backbone directed by H-bonding association was synthesized and self-assembled into cationic polymeric nanomicellar particles for in vivo antimiRNA delivery with an average diameter of 68 nm and a zeta potential of approximately 39 mV. The chemical structure of the copolymer was well characterized by 1H NMR and GPC. The cationic polymeric nanomicellar particles could be unpacked in an intracellular reductive environment (GSH) leading to the release of encapsulated antimiRNA. MTT assays in vitro showed no significant cytotoxicity of SGC7901 gastric cancer cells incubated with PEG-SS-PLA-SS-PEI micelles. The in vitro study indicated that the micelle-based antimiR-21 delivery system could effectively facilitate cellular uptake and greatly down-regulate the expression level of miR-21 in SGC7901 cell lines, which was comparable to Lipofectamine™ 2000. The down regulation of miR-21 remarkably induced apoptosis, suppressed the tumor cell migration and invasion, and increased the expression of target genes such as phosphatase and tensin homolog deleted on chromosome ten (PTEN) and Programmed Cell Death Protein 4 (PDCD4). More importantly, the in vivo systemic administration of the micelles/antimiR-21 complex in a gastric cancer model significantly inhibited tumor growth and increased the expression of target genes. The nanoparticle based on the PEG-SS-PLA-SS-PEI copolymer would be a safe and efficient carrier for delivery of therapeutic antimiRNA, which shows a prospective therapy method in gastric cancer.

Unexpected enhancement of molten salt corrosion by fission products: Superior performance of Fe-based 316L SS over Ni-based Inconel 600

Chengyu Wang,Ming Cheng,Haofeng Luo,Tao Jiang,Wentao Zhou,Yafei Wang

Molten salts are widely used in high-temperature nuclear systems, yet their interactions with structural alloys, particularly under the influence of fission product species, are still not fully understood. In this study, the corrosion behaviors of Fe-based 316L stainless steel (316L SS) and Ni-based Inconel 600 alloy were investigated in LiCl-KCl eutectic molten salt at 500 °C, under the influences of thermodynamically active lanthanide and less-active lanthanide/non-lanthanide fission product species. The results reveal that both active and less-active lanthanide species enhance the corrosion of alloys in molten salt, whereas less-active non-lanthanide barely affect the alloy’s corrosion as thermodynamically expected. The unexpected corrosion enhancement caused by less-active lanthanide species (Ce 3 + , La 3+ , Nd 3+ , Sm 3+ , Pr 3+ , Gd 3+ , and Y 3+ ) is attributed to the formation of lanthanide oxychlorides (LnOCl, Ln represents less-active lanthanide species), driven by trace oxygen present in Ar atmosphere. Furthermore, the 316L SS was found to exhibit better corrosion resistance than Inconel 600 alloy in LiCl-KCl molten salt containing fission products, despite Ni-based alloys being widely regarded as more corrosion-resistant than Fe-based alloys in molten salt environments. This study systematically investigates, for the first time, the effects of fission products on both Fe-based and Ni-based alloys in molten salt, providing a comprehensive understanding of the behavior of structural alloys for molten salt applications in the nuclear industry.

Construction of Cu2O/PANI-GO/SS flexible electrode with three-dimensional network structure towards electrochemical sensing of glucose and ascorbic acid

Haoran Li,Yuekuan Sun,Ting Xian,Jinhun Bao,Wenjuan Liu,Xiaolong Lu

Three-dimensional (3D) nanostructures which have the ability to expand carrier diffusion channels and provide plenty of electrocatalytic active sites are extensively regarded as highly effective strategies for enhancing the sensing performance of electrocatalysts. Herein, a highly sensitive Cu 2 O/PANI-GO electrode was constructed simply via in-situ electrodeposition of the graphene oxide (GO) doped polyaniline PANI (PANI-GO) 3D porous network structure on a flexible stainless-steel (SS) foil substrate. Subsequently, Cu₂O nanoparticles were electrodeposited onto the unique 3D network architecture which provides abundant active sites for Cu 2 O deposition. Meanwhile, the introduction of conductive PANI and GO improves the conductivity of the sensing catalysts and enhances the electrocatalytic performance of Cu 2 O. The electrochemical behavior of glucose and ascorbic acid (AA) was investigated through cyclic voltammetry (CV). CV results have demonstrated that the formation of a 3D porous network structure provides a substantial number of electronic pathways, which facilitate direct electron transfer between biomolecules and electrocatalysts, thereby promoting the electrocatalytic oxidation of glucose and ascorbic acid (AA). According to chronoamperometry, such a 3D porous network structure electrode exhibits outstanding sensing performance for glucose and AA detection, including lower detection limit and higher sensitivity over a wide detection range compared with the reported similar Cu 2 O-based electrodes. The excellent anti-interference, stability, reproducibility, and bending stability are also obtained. Furthermore, the glucose concentration in diluted fetal bovine serum and AA concentration in diluted vitamin c beverages were quantitatively measured, and the results were satisfactory, indicating that the proposed flexible electrochemical sensing electrode provides considerable prospects for practical human health and food safety monitoring.

Robust Alcohol Soluble Polyurethane/Chitosan/Silk Sericin (APU/CS/SS) Nanofiber Scaffolds Toward Artificial Skin Extracellular Matrices via Microfluidic Blow-Spinning

Liu Jingwen,Cui Tingting,Xu Xiaowei,Du Yu,Wang Lin,Chen Su,Pang Jie

Abstract Skin regeneration is a matter of high concern since many individuals suffer from skin damage. To date, the concept of protein-based artificial skin scaffolds have been successfully applied and proven in skin regeneration. However, realizing a skin tissue scaffold with a skin-like extracellular matrix (ECM) that combines low price, good biocompatibility, excellent antibacterial properties, good cell adhesion, and strong mechanical properties is still a major challenge. In this study, inexpensive silk sericin (SS) protein-based artificial skin nanofiber scaffolds (NFSs) with excellent biological activity, no immune rejection, and high mechanical strength were fabricated via microfluidic blow-spinning (MBS). In particular, the as-prepared NFS was transformed from a random coil structure to a \(\upbeta\) -sheet structure by using the MBS in high-speed shear chips to improve its stability and mechanical strength. Additionally, through in vitro and in vivo studies, it was shown that SS protein-based artificial skin NFSs possessed excellent antibacterial effects and degradability properties, as well as accelerated tissue granulation growth, effectively promoting full skin wound healing and skin regeneration for medical problems worldwide. Thus, this skin ECM-inspired NFS offers new perspectives for accelerating wound healing and tissue regeneration and provides potential applications for clinical medicine. Graphical abstract

Enhancement of oral bioavailability and anti-colitis effect of luteolin-loaded polymer micelles with RA (rosmarinic acid)-SS-mPEG as carrier

Zhaomin Lu,Juan Liu,Liangjian Zhao,Chenli Wang,Feng Shi,Zhengqi Li,Xuesong Liu,Zhiwei Miao

Objective Polymer micelles were prepared (L-RSPMs) with luteolin and synthetic RA-SS-mPEG polymeric material before evaluation of their anti-inflammatory effect on 2, 4, 6-trinitro-benzene-sulfonic acid (TNBS)-induced ulcerative colitis (UC) model in rats.Methods The synthetic RA-SS-mPEG was characterized with NMR spectroscopy, before preparation of luteolin-coated RA-SS-mPEG polymer micelles. The in vitro characterization and evaluation of the formulation were accomplished, couple with its pharmacokinetic parameters. The levels of PEG2, MDA, CRP and GSH, as well as concentrations of TNF-α, IL1-β, IL-6 and IL-10 in serum and colon tissue were detected via ELISA kit. The degree of colon injury and inflammation was evaluated via histopathologic examination.Results L-RSPMs displayed small average droplet size (133.40 ± 4.52 nm), uniformly dispersed (PDI: 0.163 ± 0.011), good stability, slow release and enhanced solubility. We observed 353.28% increase in the relative bioavailability of L-RSPMs compared to free luteolin, while the half-life of the micelle was extended by 6.16h. Compared to model (M) group, luteolin (low and high doses) and L-RSPMs (low and high doses) significantly reduced levels of MDA, PEG2, CRP, TNF-α, IL-6 and IL-1β in colon tissue and serum of colitic rats but dose dependently increased IL-10 and SOD levels (p < 0.01). Histopathologic examination of colon showed that luteolin (low and high doses) and L-RSPMs (low and high doses) improved colonic inflammation in colitic rats to varying degrees compared to M group.Conclusion L-RSPMs could improve TNBS-induced colon inflammation by enhancing bioavailability, promoting antioxidant effects and regulating cytokine release, which may become a potential agent for UC treatment in clinical settings.

Multifunctional Lipid Nanoparticles for Protein Kinase N3 shRNA Delivery and Prostate Cancer Therapy

Ji Wang,Yanhao Zhang,Chao Liu,Wenhui Zha,Shuo Dong,Hanlei Xing,Xinsong Li

Protein kinase N3 (PKN3), by virtue of its abnormal expression in prostate cells, has been widely used as a target of RNAi (shRNA, siRNA, miRNA) therapy. The major challenges of PKN3 RNAi therapy lie in how to design effective interference sequences and delivery systems. Herein, new PKN3 shRNA sequences (shPKN3-2459 and shPKN3-3357) were designed, and bioreducible, biodegradable, ionizable lipid-based nanoparticles were developed for shPKN3 delivery. First, an ionizable lipid (DDA-SS-DMA) bridged with disulfide bond and ester bonds was synthesized by a three-step reaction and confirmed by MS, 1H NMR, and 13C NMR. The ionizable lipid was mixed with cholesterol, DSPC, PEG-lipid, and shPKN3 by a microfluidic mixer to prepare lipid nanoparticles (LNP–shPKN3) which were characterized by DLS and TEM. Afterward, the pH and glutathione (GSH)-responsiveness of the DDA-SS-DMA based LNP delivery system were investigated by lysosome escape and gel electrophoresis assays. Compared with the commercial transfection reagent Lipo2000, the DDA-SS-DMA based delivery system showed higher transfection efficiency and lower toxicity. Western blot analysis, invasion tests, and migration assays were performed to evaluate the silencing effect of shPKN3 in vitro. In in vivo studies, high tumor suppression (65.8%) and treatment safety were evident in the LNP–shPKN3-2459 treatment group. Taken together, the DDA-SS-DMA based delivery system encapsulating shPKN3-2459 showed significant antitumor efficacy and might be a promising formulation for the treatment of prostate cancer.

Multi-omics reveals phenol-based coordinated defense of Sparganium stoloniferum rhizoma

Mengru Sang,Qinan Liu,Dishuai Li,Jingjie Dang,Shilin Dai,Chanchan Liu,Qinan Wu

Sparganium stoloniferum is a widely distributed aquatic medicinal plant that exhibits exceptional tolerance to environmental stress. Interestingly, the phenols in S. stoloniferum which have medicinal properties, are defensive compounds. Studies integrating transcriptome, proteome, and metabolome analyses have highlighted the phenylpropanoid and flavonoid biosynthesis pathways, which are related to the defense mechanisms of S. stoloniferum . During the growth process, S. stoloniferum rhizoma (SL) accumulates lignin, forming a physical defense, and phenylpropanoids and flavonoids derived during the lignin biosynthesis process provide chemical defense. In this study, a map of the molecular processes involved in the coordinated defense of S. stoloniferum was generated. The expression patterns of genes involved in the coordinated defense of SL were consistent with the omics results. The cDNA of the phenylalanine ammonia-lyase ( PAL ) gene from S. stoloniferum ( Ss PAL) was cloned. The recombinant protein Ss PAL showed good affinity and catalytic activity for L-phenylalanine. Heat stress experiments indicated that genes related to phenol biosynthesis were upregulated when S. stoloniferum responded to stress. Therefore, phenols played a crucial defense role when S. stoloniferum was at an early developmental stage or responded to stress in a short time. In contrast, the physical defense mainly relied on lignin formation, which was the main defense mechanism of SL in the late growth stage. Based on these results, we discuss the causes of phenolic pharmacological substance formation in medicinal aquatic plants. These results provide new insights into the defense strategies of aquatic plants.

Targeted multifunctional redox-sensitive micelle co-delivery of DNA and doxorubicin for the treatment of breast cancer

Longbao Feng,Shina Yan,Qiyu Zhu,Jie Chen,Lian Deng,Yanfang Zheng,Wei Xue,Rui Guo

Drug/gene co-delivery carriers are a promising strategy for cancer treatment. Thus, herein, T7-conjugated redox-sensitive amphiphilic polyethylene glycol–polyethyleneimine–poly(caprolactone)-SS-poly(caprolactone)–polyethyleneimine–polyethylene glycol (PEG–PEI–PCL-SS-PCL–PEG) (PPPT) is designed to realize the co-delivery of pORF-hTRAIL and DOX efficiently into tumor cells. PPPT is synthesized via the ring opening polymerization (ROP) of ε-caprolactone followed by Michael addition polymerization and atom transfer radical polymerization (ATRP) of the maleic imide group of MAL-PEG-NHS. The PPPT micelles present a spherical or ellipsoidal geometry with a mean diameter of approximately 100–120 nm. Meanwhile, they also exhibit a redox-responsive drug release profile in vitro. The blood compatibility and complement activation tests reveal that the PPPT micelles do not induce blood hemolysis, blood clotting, or complement activation. The T7-modified co-delivery system shows a higher cellular uptake efficiency than the unmodified co-delivery system in human breast cancer MCF-7 cells and is accumulated in tumor more efficiently in vivo. These results suggest that the T7-targeted codelivery system of DOX and pORF-hTRAIL is a combined delivery platform that can significantly improve the treatment of breast cancer.

N-Doped Carbon-Encapsulated Cu@SS-Arg Catalyst for H2 Production from Aqueous-Phase Reforming of Ethanol: Toward Carbon-Neutral Energy Cycles

Minglei Lu,Bo Zhang,Shuting Jiang,Xuezhao Yu,Yingying Zhong,Junwei Liao

The development of high-performance catalysts for aqueous-phase reforming of ethanol (APRE) is essential for advancing sustainable hydrogen production and achieving carbon neutrality. Herein, we report a N-doped carbon-encapsulated Cu nanoparticle catalyst (Cu@SS-Arg), which enhances hydrogen selectivity and suppresses carbon-containing gas byproducts. The Cu@SS-Arg catalyst, which has been optimized, achieves a H2 production rate of 31.2 μmol/gcat/s with a H2 selectivity of 99.99% at 210 °C, while concomitantly reducing CO emissions in comparison to commercial precious metal catalysts. In situ DRIFTS and TPSR-MS analyses demonstrate that the Cu+/Cu0 active sites not only promote ethanol/water activation but also facilitate the C–H/O–H bond cleavage, thus favoring the ethanol dehydrogenation (via *CH3CH2O, *CH3CHO, and *CH3COOH intermediates), water dissociation (*H2O → *OH + *H) and carbon monoxide conversion (*CO + *OH → *CO2 + *H) pathways over C–C bond cleavage. This work presents a dual strategy for designing nonprecious metal catalysts that synergistically integrates structural stabilization with N-doping regulation. Together, these approaches enable sustainable and low-carbon production of hydrogen from renewable ethanol.

Microfibrous-Structured SS-fiber@meso-HZSM-5 Catalyst for Methanol-to-Propylene: Steam-Assisted Crystallization Synthesis and Insight into the Stability Enhancement

Jia Ding,Pengjing Chen,Songyu Fan,Zhiqiang Zhang,Lupeng Han,Guofeng Zhao,Ye Liu,Yong Lu

Free-standing stainless-steel (SS)-fiber@meso-HZSM-5 core–shell catalysts engineered from micro- to macro-scale were highly efficiently synthesized via cost-effective steam-assisted crystallization (SAC) method. Impact of synthesis conditions on their morphology and textural/acidic properties was investigated by means of XRD, SEM, TEM, solid-state NMR, NH3-TPD, Py-IR and N2 physisorption. Single-run lifetime of such structured catalysts for MTP process was strongly dependent on their preparation conditions but slightly the product distribution. A volcano-like relationship for lifetime was observed against the SAC time, which was correlated well with the crystallization-time-dependent crystallinity, mesoporosity and crystal size. The promising SS-fiber@meso-HZSM-5 was the one obtained after the SAC for 12 h, which delivered a prolonged single-run lifetime of 620 h with a high propylene selectivity of ∼42% at 450 °C using a methanol weight hourly space velocity (WHSV) of 1 h–1, as the result of high crystallinity, well-developed mesoporosity and small crystal size. Note that well-developed mesoporosity was paramount for the catalyst stability improvement, because of enhanced accommodation capacity of zeolite shell for receiving formed coke.