JOURNAL OF CHROMATOGRAPHY A
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2023
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IF 4.1
Nasir Ali,Fuyi Wang,Li Qi
Fabricating polymeric coatings that are responsive to multiple/dual stimuli is crucial and remains a major challenge in the development of highly efficient open tubular capillary electrochromatography (OT-CEC). In this study, a pH and temperature-responsive block copolymer, poly(styrene-maleic anhydride 2-dimethylamino ethyl methacrylate), P(St-MAn-DMAEMA), was designed and synthesized. Using P(St-MAn-DMAEMA) as the coating, an OT-CEC protocol was constructed for the analysis of chromones. The morphology and hydrophobicity-hydrophilicity of the polymeric coating could change via varying the environmental conditions, affecting the separation efficiency of OT-CEC. Interestingly, the best performance of OT-CEC was achieved at pH 9.7 and 45 °C via tuning the interactions between the coating and the analytes. Additionally, the proposed OT-CEC method exhibited a good linear range for the detection of the three test chromones from 10.0 to 100.0 μM, with all correlation coefficients (R 2 ) >0.997. The coatings also had good stability and reusability. This work provides an approach for the preparation of new multiple-stimuli-responsive polymeric coatings for the establishment of OT-CEC systems.
研究领域:
分析化学
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高分子科学
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电化学
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产品关联
DOI
PubMed
高影响因子
Bioactive Materials
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2022
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IF 16.874
Jianhua He,Wenli Zhang,Xiaoju Zhou,Fengfei Xu,Jiahui Zou,Qiqi Zhang,Yi Zhao,Hongliang He,Hu Yang,Jianping Liu
Nanoparticle-based therapeutics represent potential strategies for treating atherosclerosis; however, the complex plaque microenvironment poses a barrier for nanoparticles to target the dysfunctional cells. Here, we report reactive oxygen species (ROS)-responsive and size-reducible nanoassemblies, formed by multivalent host-guest interactions between β-cyclodextrins (β-CD)-anchored discoidal recombinant high-density lipoprotein (NP 3 ST ) and hyaluronic acid-ferrocene (HA-Fc) conjugates. The HA-Fc/NP 3 ST nanoassemblies have extended blood circulation time, specifically accumulate in atherosclerotic plaque mediated by the HA receptors CD44 highly expressed in injured endothelium, rapidly disassemble in response to excess ROS in the intimal and release smaller NP 3 ST , allowing for further plaque penetration, macrophage-targeted cholesterol efflux and drug delivery. In vivo pharmacodynamicses in atherosclerotic mice shows that HA-Fc/NP 3 ST reduces plaque size by 53%, plaque lipid deposition by 63%, plaque macrophage content by 62% and local inflammatory factor level by 64% compared to the saline group. Meanwhile, HA-Fc/NP 3 ST alleviates systemic inflammation characterized by reduced serum inflammatory factor levels. Collectively, HA-Fc/NP 3 ST nanoassemblies with ROS-responsive and size-reducible properties exhibit a deeper penetration in atherosclerotic plaque and enhanced macrophage targeting ability, thus exerting effective cholesterol efflux and drug delivery for atherosclerosis therapy.
研究领域:
纳米技术
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药学
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心血管疾病
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药物递送系统
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2022
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IF 9.965
Mengmeng Xu,Yuan Liu,Wanru Luo,Fangrong Tan,Donghua Dong,Weichang Li,LinGe Wang,Qianqian Yu
A multi-functional nanocatalytic system based on combined therapies has attracted considerable research attention in recent years due to its potential in the treatment of cancer. Herein, ZnO 2 @ [email protected] nanoparticles (NPs) based on hydroxyl radical (•OH) mediated chemodynamic therapy (CDT) and glucose-exhausting starvation therapy (ST) were constructed. Specifically, in the acidic tumor microenvironment (TME), the pH responsive decomposition of the shell ZIF-67 triggered the release of the Fenton-like catalyst Co 2+ , after which the exposed zinc peroxide (ZnO 2 ) reacted with H 2 O (H + ) to generate O 2 and hydrogen peroxide (H 2 O 2 ). The generated O 2 could alleviate hypoxia in the TEM and interact with ultra-small Au NPs originally coated on ZnO 2 to catalyze intracellular glucose and to produce another source of H 2 O 2 . While the glucose consumption caused the starvation of tumor cells, the generated H 2 O 2 from dual sources reacted with the catalyst Co 2+ to generate highly toxic •OH for CDT. Systematic in vitro and in vivo experiments were carried out to evaluate this nanocatalytic system, and the results showed an enhanced efficacy of this cancer therapy.
研究领域:
癌症治疗
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纳米技术
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生物医学工程
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材料科学
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化学工程
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
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2024
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IF 6.7
Shuang Yue,Xiaotong Wang,Lei Wang,Jiajia Li,Yufeng Zhou,Yan Chen,Zeyang Zhou,Xiaodong Yang,Xiaofeng Shi,Song Gao,Zhongmin Wen,Xiaojun Zhu,Yan Wang,Shuang Yang
The Tn antigen, an immature truncated O-glycosylation, is a promising biomarker for cancer detection and diagnosis. However, reliable methods for analyzing O-GalNAcylation and complex O-glycosylation are lacking. Here, we develop a novel method, MOTAI, for the sequential analysis of O-glycosylation using different O-glycoproteases. MOTAI conjugates glycopeptides on a solid support and releases different types of O-glycosylation through sequential enzymatic digestion by O-glycoproteases, including OpeRATOR and IMPa. Because OpeRATOR has less activity on O-GalNAcylation, MOTAI enriches O-GalNAcylation for subsequent analysis. We demonstrate the effectiveness of MOTAI by analyzing fetuin O-glycosylation and Jurkat cell lines. We then apply MOTAI to analyze colorectal cancer and benign colorectal polyps. We identify 32 Tn/sTn-glycoproteins and 43 T/sT-glycoproteins that are significantly increased in tumor tissues. Gene Ontology analysis reveals that most of these proteins are ECM proteins involved in the adhesion process of the intercellular matrix. Additionally, the protein disulfide isomerase CRELD2 has a significant difference in Tn expression, and the abnormally glycosylated T345 and S349 O-glycosylation sites in cancer group samples may promote the secretion of CRELD2 and ultimately tumorigenesis through ECM reshaping. In summary, MOTAI provides a powerful new tool for the in-depth analysis of O-GalNAcylation and complex O-glycosylation. It also reveals the upregulation of Tn/sTn-glycoproteins in colorectal cancer, which may provide new insights into cancer biology and biomarker discovery.
研究领域:
癌症生物学
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糖生物学
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生物化学
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分子生物学
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临床诊断
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产品关联
DOI
PubMed
高影响因子
Journal of Environmental Chemical Engineering
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2021
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IF 5.909
Yaowen Liu,Xiaorong Dong,Kaiwen Bao,Zhiyong Deng,Md Alomgir Hossen,Jianwu Dai,Wen Qin,KangJu Lee
In this study, potassium persulfate, N,N′-methylene bisacrylamide (MBA), and soluble starch (ST) were in situ cross-linked into porous antimicrobial starch particles containing N-halamine functional groups (PST–MBA–Cl). Then, polyacrylonitrile (PAN)/silica aerogel (SA)/zinc oxide (ZnO) beaded fibers containing PST–MBA–Cl particles were prepared by electrospinning. Scanning electron microscopy results demonstrated the uniform dispersion of the PST–MBA–Cl particles and ZnO on the surface and interior of the beaded fiber when the concentration of the PST–MBA–Cl particles was 15%. The composite beaded fibers prepared after chlorination and soaking in zinc salt solutions of different pH values had a higher degree of interior fluffiness. For the analysis of the effectivity of the prepared particles using 10 8 CFU/mL gram-positive ( S. aureus ) and gram-negative ( E. coli ) bacterial human pathogens , the inhibition zone was 15.97 ± 0.12 and 17.81 ± 0.23 mm, respectively. Meanwhile, the degradation efficiency of PAN and SA for the cationic dye methylene blue (MB) was 88.00% ± 1.83%. The PAN/SA/PST–MBA–Cl/ZnO composite beaded fiber showed good antibacterial properties and a fast recovery rate, and its degradation efficiency for MB was retained at ~ 80% after five cycles. Thus, the PAN/SA/ PST–MBA–Cl/ZnO beaded fibers can be used as an environment-friendly and reusable adsorbing material for the removal of dyes from industrial wastewater .
研究领域:
环境科学
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材料科学
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化学工程
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水处理
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）