SURFACE & COATINGS TECHNOLOGY
·
2019
·
IF 3.192
Xiaohua Zhou,Jianan OuYang,Li Li,Qing Liu,Congcong Liu,Mingying Tang,Youwen Deng,Ting Lei
Mg alloy is an excellent biomaterial due to its ideal mechanical properties , bio-degradability and bio-compatibility. However, high local pH, subcutaneous gas pockets and compromised mechanical integrity caused by high degradation rate restrict its clinical application. The purpose of this study is to improve the anti-corrosion properties of 5β-TCP/Mg-3Zn scaffold using poly-dopamine/gelatin (PDA/G) composite coatings through an easy, convenient and cheap dipping method. We found that dopamine (C 8 H 11 NO 2 ) slowed down corrosion by forming thin, surface-adherent PDA films on the scaffold surface. Gelatin formed a dense organic membrane cover on the rough surface which results from the processing of PDA membrane to seal the macrocracks and protect it from corrosion. As compared to bare 5β-TCP/Mg-3Zn alloy, electrochemical tests showed that the corrosion current density ( i corr ) decreased from 5.24 × 10 −3 A/cm 2 to 1.41 × 10 −3 A/cm 2 , indicating improved anti-corrosion properties. The soaking results indicated that pH values of immersion solution, as well as the volumes of released hydrogen, were 9.83 and 9.25, 20 mL and 15 mL for bare and coated alloy, respectively, indicating that the composite coating enhanced the anti-corrosion property of Mg alloy. The in vitro cytotoxicity test showed that 10%, 50% and 100% concentration of the immersion solution were safe for cellular applications. The in vivo test showed that serum magnesium concentration for PDA/G was lower than that of the bare sample. In addition, the materials and coatings were nontoxic to muscle and other vital organs, including heart, liver, kidney, and muscle. Moreover, the PDA/G composite coating showed a better anti-corrosion property in vivo than the bare sample and displayed a longer service time. These results suggest that coating of 5β-TCP/Mg-3Zn scaffold with PDA/G slowed down the corrosion rate of Mg alloy.
研究领域:
生物材料
·
腐蚀科学
·
生物医学工程
✓ 已验证
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2023
·
IF 13.6
Jinfan Zhang,Zili Lin,Zongshun Yu,Yudan Zhang,Danluo Liang,Yingyi Chen,Yu Chen,Ping Chen,Haijin Liu,Wenying Lv,Guoguang Liu
For this work, we employed n-type Bi 2 WO 6 and p-type PhC 2 Cu to formulate a direct Z-scheme Bi 2 WO 6 /PhC 2 Cu (PCBW) photocatalyst via simplified ultrasonic stirring technique. An optimal 0.6PCBW composite exhibited the capacity to rapidly photodegrade 2,4,6-TCP (98.6% in 120 min) under low-power blue LED light, which was 8.53 times and 12.53 times faster than for pristine PhC 2 Cu and Bi 2 WO 6 , respectively. Moreover, electron spin resonance (ESR), time-resolved PL spectra, and quantitative ROS tests indicated that the PCBW enhanced the separation capacity of photocarriers. It also more readily associated with dissolved oxygen in water to generate reactive oxygen species (ROS). Among them, the ability of PCBW to produce ·O 2 - in one hour was 12.07 times faster than for pure PhC 2 Cu. In addition, the H 2 O 2 formation rate and apparent quantum efficiency of PCBW are 10.73 times that of PhC 2 Cu, which indicates that PCBW not only has excellent photocatalytic performance, but also has outstanding ROS production ability. Furthermore, Ag photodeposition, in situ X-ray photoelectron spectroscopy (XPS) and density functional theory (DFT) calculations were utilized to determine the photogenerated electron migration paths in the PCBW, which systematically confirmed that Z-scheme heterojunction were successfully formed. Finally, based on the intermediate products, three potential 2,4,6-TCP degradation pathways were proposed.
研究领域:
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANNALS OF BOTANY
·
2024
·
IF 3.6
Guo Huanhuan,Li Shiqin,Liu Yong,Yang Qinsong
Background and AimsChinese pistachio (Pistacia chinensis Bunge), an important horticultural plant species, holds great ornamental value with beautiful leaves and fruits. Seedling propagation of this tree species is restricted by its erratic seed germination, however, the germination mechanism is ambiguous yet. The aim of this study was to figure out the germination mechanism from the novel perspective based on the multi-omics data.MethodsThe multi-omics technique combined with hormone content measurement was first applied in seed germination of Chinese pistachio.Key ResultsDue to the great accumulation during seed germination, catechin stood out from the identified metabolites by broadly targeted metabolomic analysis. Exogenous catechin of 10 mg/L significantly improved the germination of Chinese pistachio seeds. An interesting result of hormone analysis showed that the improving effect of catechin could be attributed to increase of the gibberellic acid 3 (GA3) content rather than decrease of the abscisic acid (ABA) content before germination. The paclobutrazol (PAC, a GA biosynthesis inhibitor) and PAC + catechin treatments also showed that the promoting effect of catechin on seed germination depends on GA biosynthesis. Transcriptome analysis and qRT‒PCR further revealed that catechin induced the expression of PcGA20ox5 to activate GA biosynthesis. Several transcription factors were induced by catechin and GA treatments, such as TCP, bZIP and C3H, which may play an important regulatory role in GA biosynthesis in a catechin-mediated way.ConclusionsCatechin promotes seed germination via GA biosynthesis in Chinese pistachios. This study proposes a novel mechanism by which catechin promotes seed germination via the GA pathway, which provides new insight into a comprehensive understanding of seed dormancy and germination.
研究领域:
植物学
·
植物生理学
·
分子生物学
·
园艺学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2022
·Vol. 10
·期 17
·3344-3356
·
IF 7.571
Linxue Zhang,Zhuo Wan,Zuoying Yuan,Jun Yang,Yunfan Zhang,Qing Cai,Jianyong Huang,Yuming Zhao
Multicellular aggregates have been widely utilized for regenerative medicine; however, the heterogeneous structure and undesired bioactivity of cell-only aggregates hinder their clinical translation. In this study, we fabricated an innovative kind of microparticle-integrated cellular aggregate with multifunctional activities in angiogenesis and osteogenesis, by combining stem cells from human exfoliated deciduous teeth (SHEDs) and bioactive composite microparticles. The poly(lactide-co-glycolide) (PLGA)-based bioactive microparticles (PTV microparticles) were ∼15 μm in diameter, with dispersed β-tricalcium phosphate (β-TCP) nanoparticles and surface-modified vascular endothelialcadherin fusion protein (hVE-cad-Fc). After co-culturing with microparticles in U-bottomed culture plates, SHEDs could firmly attach to the microparticles with a homogeneous distribution. The PTV microparticle-integrated SHED aggregates (PTV/SHED aggregates) showed significant positive CD31 and ALP expression, as well as the significantly upregulated osteogenesis makers (Runx2, ALP, and OCN) and angiogenesis makers (Ang-1 and CD31), compared with PLGA, PLGA/β-TCP (PT) and PLGA/hVE-cad-Fc (PV) microparticle-integrated SHED aggregates. Finally, in mice, 3 mm calvarial defects filled with the PTV microparticle-integrated SHED aggregates achieved abundant vascularized neo-bone regeneration within 4 weeks. Overall, we believe that these multifunctional PTV/SHED aggregates could be used as modules for bottom-up regenerative medicine, and provide a promising method for vascularized bone regeneration.
研究领域:
再生医学
·
组织工程
·
生物材料
·
干细胞生物学
✓ 已验证
DOI
PubMed
高影响因子
CERAMICS INTERNATIONAL
·
2026
·
IF 6
Yanlong Wu,Jiangyong Wu,Jianfeng Kang,Jingwei Yang,Shuanglong Zhang,Jian Qiao,Xiaoxi Shao,Xu Chen
Bioactive ceramics are ideal materials for bone substitutes owing to their excellent osteoconductivity, but the application in load-bearing conditions is limited by their intrinsic brittleness. Inspired by the interpenetrating organic-inorganic structure of natural bone, we developed ceramic/polymer interpenetrating phase composites (C/P-IPCs) by vacuum-assisted microporous infiltration of polycaprolactone (PCL) molten into the interconnected micropores in vat photopolymerization printed β-tricalcium phosphate (β-TCP) samples. The C/P-IPCs achieved a synergy of mechanical properties and bioactivity compared with the pure ceramic β-TCP and the conventional ceramic particle-reinforced polymer (CPR-P) from the same materials. Specifically, the composites exhibited dramatically enhanced mechanical properties over pure ceramic, with strength increases of approximately 2.1- to 2.4-fold and strain energy density improvements of 2.4- to 5.5- fold. Meanwhile, the C/P-IPCs demonstrated a moderate degradation rate and superior mechanical stability during degradation. Furthermore, their biological performance of C/P-IPCs was markedly superior to that of CPR-P, including cell proliferation, differentiation, and bone repair. These results indicate that the C/P-IPCs are a promising candidate for bone repair under load-bearing conditions.
✓ 已验证
DOI
高影响因子
PLoS One
·
2020
·Vol. 15
·期 1
·e0228247
·
IF 2.74
Congcong Liu,Jingcheng Wang,Chengde Gao,Zhenting Wang,Xiaohua Zhou,Mingying Tang,Kun Yu,Youwen Deng
Magnesium-based biomaterials are attracting increasingly more attention for orthopedic applications based on their appropriate mechanical properties, biodegradability, and favorable biocompatibility. However, the high corrosion rate of these materials remains to be addressed. In this study, porous β-Ca 3 (PO 4 ) 2 /Mg-Zn (β-TCP/Mg-Zn) composites were fabricated via a powder metallurgy method. The β-TCP/Mg-Zn composites with 6% porosity exhibited optimal mechanical properties, and thus, they were selected for surface modification. A novel dopamine/gelatin/recombinant human bone morphogenetic protein-2 (rhBMP-2) coating with demonstrated stability was prepared to further improve the corrosion resistance of the composite and enhance early osteoinductivity. The homogeneously coated β-TCP/Mg-Zn composite showed significantly improved corrosion resistance according to electrochemical and immersion tests. In addition, extracts from the dopamine/gelatin/rhBMP-2–coated β-TCP/Mg-Zn composite not only facilitated cell proliferation but also significantly enhanced the osteogenic differentiation of Sprague–Dawley rat bone marrow-derived mesenchymal stem cells in vitro . Furthermore, in vivo experiments were performed to evaluate the biodegradation, histocompatibility, and osteoinductive potential of the coated composite. No obvious pathological changes in the vital visceral organs were observed after implantation, and radiography and hematoxylin-eosin staining showed strong promotion of new bone formation, matched composite degradation and bone regeneration rates, and complete absorption of the released hydrogen gas. Collectively, these results indicate that the dopamine/gelatin/rhBMP-2–coated β-TCP/Mg-Zn composite offers improved corrosion resistance, favorable biocompatibility, and enhanced osteoinductive potential for use in the fabrication of orthopedic implants.
研究领域:
材料科学
·
生物医学工程
·
骨科
✓ 已验证
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2021
·
IF 7.086
Bingzhi Liu,Tingyu Pan,Jiajun Liu,Li Feng,Yuning Chen,Huaili Zheng
Chlorophenol is a widely used organic compound, and the environmental and health problems caused by it have being worsened in recent years. This study used 2,4,6-trichlorophenol (2,4,6-TCP) as the target pollutant, and employed ultrasound (US) enhanced zero-valent iron (Fe 0 )/EDTA/air system (FEA), namely US/FEA, to remove 2,4,6-TCP. The influence of single factor experimental conditions such as EDTA concentration, Fe 0 dosage, US power, pH and pollutant concentration on the removal efficiency of 2,4,6-TCP was investigated, and the optimal reaction conditions were determined. The mechanism of reactive oxygen species (ROS) produced by US/FEA was explored. The degradation process and removal mechanism of 2,4,6-TCP in the US/FEA were discussed through the determination and analysis of intermediate products. The results showed that US could continuously activate and renew the Fe 0 surface, accelerate its oxidation and corrosion process, and then continuously and stably produce sufficient amounts of Fe 2+ and Fe 3+ . Ultrasonic cavitation effect could reduce the difficulty of O 2 activation reaction, and promote the production of sufficient H 2 O 2 . The addition of EDTA made the system have a wide range of pH applications, and its performance under neutral and alkaline conditions was also superior. The ROS of US/FEA included ·OH, O 2 ·- and Fe(IV), where Fe(IV) was the main contributor to the removal of 2,4,6-TCP. In addition, the degradation of 2,4,6-TCP had two processes including dechlorination and benzene ring opening. First, 2,4,6-TCP was dechlorinated and degraded into phenol. And then, phenol was degraded into small molecular acids by ring-opening, and finally it was mineralized into CO 2 and H 2 O completely. US/FEA is a promising technology for high-efficiency degradation of organic matter and deep environmental purification.
研究领域:
环境科学
·
化学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MATERIALS & DESIGN
·
2023
·
IF 8.4
Hao Wang,Xinyu Xu,Xiaohan Mei,Dapeng Zeng,Boda Ying,Zehao Yu,Shibo Liu,Ruiyan Li,Yanguo Qin
Bone implants that promote osseointegration and have antimicrobial capabilities are necessary to ensure optimal long-term functionality of implants. In this study, a porous composite osteogenic scaffold was prepared using a twin-screw extruder and a fused deposition modeling three-dimensional (3D) printer. A polydopamine (PDA) coating was used to load graphdiyne oxide (GDYO) on the surface of the 3D printed polyetherimide-tricalcium phosphate (PEI-TCP) osteogenic scaffold through π–π double-bond interactions. The resulting P-T-GDYO composite scaffold was characterized, and its mechanical properties, ability to promote osteogenic differentiation, and light-responsive antibacterial activity were evaluated. When the scaffold was doped with 10 wt% β-TCP, its mechanical properties remained in the range of those of cancellous bone. In terms of osteogenic differentiation, the P-T-GDYO composite scaffold promoted adhesion and osteogenic differentiation of MC3T3-E1 cells, as well as effectively promoting endogenous bone regeneration of rabbit femoral condyle bone defects in osteogenesis experiments in vivo . In a mouse subcutaneous infection model, after near-infrared light irradiation, P-T-GDYO showed an antibacterial rate of 90.18 % against Staphylococcus aureus , while maintaining good tissue–organ compatibility. This study represents an important development in GDYO-based photothermal antibacterial materials in the field of orthopedics and expands its application in the biomedical field.
研究领域:
生物材料学
·
骨科医学
·
组织工程学
✓ 已验证
产品关联
DOI
高影响因子
EUROPEAN POLYMER JOURNAL
·
2024
·
IF 5.8
Yuxin Niu,Fuxiang Song,Rong Shan,Qianjuan Zhao,Zhuang Chen,Na Wang,Weibo Xie
The use of 3D printing technology has advanced the bone tissue engineering, and constructing large artificial bones for repairing large-scale bone defects is highly significant. This study aimed to construct large artificial bones in a precise and controllable manner, focusing on repairing critical-sized bone defects. The researchers used 3D printing technology to synthesize 3D PCL/β-TCP, and then evaluated its ability to promote MC3T3-E1 cell adhesion, proliferation, and new bone formation through a series of characterizations. The results confirmed that 3D PCL/β-TCP, presented as a lattice structure similar to natural bone, could be used to prepare personalized artificial bone blocks based on the actual range of bone defects. The researchers also experimented with dipping 3D PCL/β-TCP bone blocks in different proportions of β-TCP and found that PT2 showed better mechanical and hydrophilic properties. Further experiments showed that PT2 presented excellent biocompatibility and outstanding capacity to promote MC3T3-E1 cell proliferation and adhesion, and that 3D PCL/β-TCP bone blocks possessed good osteogenic activity. Finally, it was suggested that PT2 exhibited the property of promoting new bone formation. These findings provide experimental data support for the repair of critical-sized bone defects.
✓ 已验证
产品关联
DOI
高影响因子
NEW PHYTOLOGIST
·
2024
·
IF 8.3
Honglian Gu,Jiaxing Li,Dahe Qiao,Mei Li,Yingjie Yao,Hui Xie,Ke-lin Huang,Shengrui Liu,De-Yu Xie,Chaoling Wei,Junyan Zhu
Summary Numerous herbivore-induced plant volatiles (HIPVs) play important roles in plant defense. In tea plants ( Camellia sinensis ), (Z)-3-hexenyl acetate (3-HAC) has been characterized as associated with resistance to herbivores. To date, how tea plants biosynthesize and regulate 3-HAC to resist herbivores remain unclear. Based on transcriptomes assembled from Ectropis obliqua- fed leaves, a cDNA encoding BAHD acyltransferase, namely CsCHAT1 , was highly induced in leaves fed with E. obliqua . Enzymatic assays showed that CsCHAT1 converted (Z)-3-hexenol into 3-HAC. Further suppression of CsCHAT1 expression reduced the accumulation of 3-HAC and lowered the resistance of tea plants to E. obliqua , while 3-HAC replenishment rescued the reduced resistance of CsCHAT1 -silenced tea plants against E. obliqua . Two transcription factors (TFs), CsNAC30 and CsTCP11 , were co-expressed with CsCHAT1 . An integrative approach of biochemistry, DNA–protein interaction, gene silencing, and metabolic profiling revealed that the two TFs positively regulated the expression of CsCHAT1 . The suppression of either one decreased the production of 3-HAC and eliminated the resistance of tea plants to E. obliqua . Notably, the suppression of either one considerably impaired JA-induced 3-HAC biosynthesis in tea plant. The proposed pathway can be targeted for innovative agro-biotechnologies protecting tea plants from damage by E. obliqua .
研究领域:
植物学
·
分子生物学
·
农业科学
✓ 已验证
DOI
PubMed
高影响因子
Bioactive Materials
·
2023
·
IF 18.9
Qiji Lu,Jingjing Diao,Yingqu Wang,Jianlang Feng,Fansen Zeng,Yan Yang,Yudi Kuang,Naru Zhao,Yingjun Wang
Bone bionics and structural engineering have sparked a broad interest in optimizing artificial scaffolds for better bone regeneration. However, the mechanism behind scaffold pore morphology-regulated bone regeneration remains unclear, making the structure design of scaffolds for bone repair challenging. To address this issue, we have carefully assessed diverse cell behaviors of bone mesenchymal stem cells (BMSCs) on the β-tricalcium phosphate (β-TCP) scaffolds with three representative pore morphologies (i.e., cross column, diamond, and gyroid pore unit, respectively). Among the scaffolds, BMSCs on the β-TCP scaffold with diamond pore unit (designated as D-scaffold) demonstrated enhanced cytoskeletal forces, elongated nucleus, faster cell mobility, and better osteogenic differentiation potential (for example, the alkaline phosphatase expression level in D-scaffold were 1.5–2 times higher than other groups). RNA-sequencing analysis and signaling pathway intervention revealed that Ras homolog gene family A (RhoA)/Rho-associated kinase-2 (ROCK2) has in-depth participated in the pore morphology-mediated BMSCs behaviors, indicating an important role of mechanical signaling transduction in scaffold-cell interactions. Finally, femoral condyle defect repair results showed that D-scaffold could effectively promote endogenous bone regeneration, of which the osteogenesis rate was 1.2–1.8 times higher than the other groups. Overall, this work provides insights into pore morphology-mediated bone regeneration mechanisms for developing novel bioadaptive scaffold designs.
研究领域:
生物医学工程
·
组织工程
·
细胞生物学
·
再生医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2024
·
IF 18.5
Na Wen,Shuangshuang Li,Hongzhi Jiang,Jiachao Yang,Weibo Yang,Yunhao Song,Jinlin Long,Jiwu Zhao,Zhihui Lin,Xunbin Yu,Yen Wei,Shiyun Lu,Xueping Huang,Tianhua Zhou
Gastric ulcers accompanied by acute or chronic bleeding represent a significant risk of fatality. However, the development of effective strategies for achieving rapid hemostasis and wound healing in gastric ulcer bleeding represents a formidable challenge. To address this challenge, thrombin-derived C-terminal peptide (TCP-25) is employed as a targeted agent, and it is incorporated into two novel injectable and biocompatible carboxymethyl chitosan (CMCs) hydrogels via Schiff's base reaction. The TCP-25 peptide hydrogels display optimal adaptability within the distinctive gastrointestinal microenvironment, demonstrating a balance of acid resistance and degradation, along with controlled release and bioactivity, and good bio-adhesive properties. Both in vitro and in vivo reveal that the hydrogel achieves rapid hemostasis. In an ethanol-induced gastric ulcer model in rats, it demonstrates that the hydrogel effectively inhibits gastric ulcer bleeding by 92% within 24-h, exhibiting efficacy that surpasses that of omeprazole medicine, a commonly used clinical treatment. This enhanced performance is attributed to the synergistic effects of the CMCs hydrogel and TCP-25 peptide in effectively achieving hemostasis, inhibiting bacterial growth, and promoting gastric wound healing in acute or chronic bleeding gastric ulcers. Consequently, the present work offers promising clinical applications for rapid hemostasis and wound healing in gastric ulcers.
研究领域:
生物医学工程
·
材料科学
·
药学
·
胃肠病学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）