CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
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2013
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IF 0.769
Yu-Rong TANG,Yu ZHANG,Rui LIU,Ying-Ying SU,Yi LÜ
The poly(acrylic acid) functionalized NaYF 4 :Yb,Er nanoparticles (NPs) were found to possess intrinsic peroxidase-like activity, which could catalyze oxidization of 3,3′,5,5′-tetramethylbiphenyl (TMB) by H 2 O 2 to produce a color variation. Using TMB as substrate, we systematically studied the effect of a series of conditions, such as temperature and pH, on the catalytic activity of NaYF 4 :Yb,Er NPs. When combined with uricase, the proposed colorimetric method could be successfully used for the determination of uric acid. Under the optimum conditions, the linear range of this method was from 1.0 × 10 −5 M to 2.0 × 10 −4 M with the detection limit down to 5.3 × 10 −6 M. Moreover, the developed method was applied to detect uric acid in serum samples and the results were in good agreement with those reported by the hospital.
研究领域:
生物化学
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医学诊断
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纳米技术
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产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Yanzi Wu,Yujie Ma,Guanhong Xu,Fangdi Wei,Yunsu Ma,Quan Song,Xu Wang,Tang Tang,Yueyue Song,Menglan Shi,Xiaoman Xu,Qin Hu
Artificial enzymes have received much attention because of easy access, low cost and good stability. In this work, we report on a core–shell artificial peroxidase, namely, Fe 3 O 4 @MIL-100(Fe), which can catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) by H 2 O 2 to produce a blue product. The combination of Fe 3 O 4 and MIL-100(Fe) can remarkably enhance peroxidase-like activity. The catalytic behavior of Fe 3 O 4 @MIL-100(Fe) followed Michaelis–Menten equation and the affinity of Fe 3 O 4 @MIL-100(Fe) to substrate was higher than horseradish peroxidase. Under the optimal conditions, the absorbance of oxidized TMB at 652 nm is linearly correlated with the concentration of H 2 O 2 . As H 2 O 2 is the oxidative product of cholesterol in the presence of cholesterol oxidase, a sensitive and selective method of cholesterol detection was also developed. As low as 0.8 μM of cholesterol could be detected with a linear range from 2 to 50 μM ( r = 0.999). The proposed method was successfully applied to the detection of cholesterol levels in serum samples. As an artificial peroxidase, Fe 3 O 4 @MIL-100(Fe) has advantages of high catalytic efficiency, low cost, good stability, easy preparation and storage. This work demonstrates that MOF-based materials hold great promise for applications in bioanalysis.
研究领域:
分析化学
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生物化学
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纳米技术
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材料科学
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产品关联
DOI
高影响因子
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
·
2017
·
IF 0.795
Liang-Liang WU,Zhi-Juan QIAN,Zheng-Jun XIE,Ying-Ying ZHANG,Chi-Fang PENG
A sensitive colorimetric method for detection of copper ions (Cu 2+ ) was developed based on surface modification of Ag/Pt nanoclusters (Ag/Pt NCs) and regulation of the peroxidase-like activity. It was found that 3-mercaptopropionic acid (MPA) could inhibit the catalytic ability of Ag/Pt NCs, however it would lose the inhibition toward catalytic ability of Ag/Pt NCs after oxidization by oxygen through the catalysis of Cu 2+ . On the basis of this, a colorimetric method was developed for the detection of Cu 2+ by measuring the colorimetric signal variation of the TMB-H 2 O 2 reaction. This method exhibited high sensitivity and selectivity toward Cu 2+ over a panel of other metal ions, in the linear range of 10–100 nM with a detection limit of 5.0 nM. The method was simple and low cost, and suitable for Cu 2+ detection in real water samples.
研究领域:
分析化学
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纳米技术
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环境科学
✓ 已验证
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2018
·
IF 6.042
Zhonghui Chen,Chaoqun Chen,Huawei Huang,Fang Luo,Longhua Guo,Lan Zhang,Zhenyu Lin,Guonan Chen
Hydrogen sulfide (H2S) is important for normal neural functions, which involves protecting neurons from oxidative stress and neuronal transmission modulation in brain. The detection of H2S is significant for revealing its role in the diagnosis of various disease. In this study, a novel multicolor colorimetric assay based on the etching of gold nanorods (Au NRs) is proposed to evaluate H2S level with the naked eye. This measurement relies on the catalytic oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) via horseradish peroxidase (HRP) to produce TMB2+, which could etch the Au NRs quickly and accompany with a distinct color change. The vivid colors can be easily distinguished with the naked eye without any sophisticated instruments. The presence of H2S can cause the deactivation of HRP, which affects the amount of TMB2+ produced and consequently affects the color changing of the system. Based on this mechanism, a simple but sensitive multicolor colorimetric assay is developed for H2S detection with a linear range of 0.05–50 μM. The proposed method is demonstrated for monitoring extracellular H2S in rat brain coupled with microdialysate.
研究领域:
分析化学
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神经科学
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2021
·Vol. 13
·期 48
·5851-5858
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IF 2.896
Yan Guan,Xiao-Lan Zhao,Qiu-Xia Li,Long Huang,Jian-Mei Yang,Tong Yang,Yun-Hui Yang,Rong Hu
In this paper, a novel colorimetric strategy based on iodide ion (I−) and Cu-MOF catalysis was developed for simple, low-cost, and naked-eye detection of Fe3+. Both I− and MOFs display catalytic activity toward peroxidase substrate 3,3′,5,5′-tetramethylbenzidine (TMB). Adsorption and embedding of I− in Cu-MOF generates Cu-MOF/I with a three-dimensional porous structure that exhibits higher specific surface area, providing more active sites to facilitate interaction with TMB, resulting in enhanced catalytic efficiency. Reports have shown that Fe3+ can oxidize TMB in the absence of H2O2. We found that as Fe3+ concentration increases, the color of the system gradually deepens and the UV absorption peak gradually increases, thus providing a colorimetric sensor for quantitative Fe3+ detection. The detection limit (LOD) obtained in the presence of I− is 200 nM; however, in the absence of I−, the LOD is approx. 10 μM. Thus, the sensing system is ideal for signal amplified analysis of Fe3+. In the presence of various interfering metal ions, the developed sensing system displays excellent selectivity. Additionally, the practical application to Fe3+ detection in real samples is explored.
研究领域:
分析化学
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材料科学
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纳米技术
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环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Miao Ma,Qing Wang,Qianqian Su,Weizhe Liu,Hui Xia,Caixia Zhou,Mengtian Li,Fang Yang,Fujian Xu
Blood uric acid (UA) levels are closely associated with various diseases. This study presents a novel UA photothermal detection system. UA reacts with uricase to generate hydrogen peroxide, which then oxidizes 3,3′,5,5′-tetramethylbenzidine (TMB) into oxidized TMB (oxTMB) in the presence of copper ions as the catalyst. Crucially, instead of expensive laser, Light Emitting Diode (LED) was applied to offer the irradiation on oxTMB, inducing a temperature increase. The increment of temperature measured by a handheld household thermometer exhibits a linear correlation with the concentration of UA in the samples. This method demonstrates enhanced detection sensitivity and excellent anti-interference capabilities. It has been successfully applied to precisely determine UA levels in volunteer blood samples. The sensing platform achieves portable UA detection in human blood within 3 min, featuring a linear response range of 50–400 μM, a detection limit (LOD) of 14.4 μM. This work establishes a novel miniaturized LED-based photothermal (LED-PT) uric acid detection device, holding significant potential for rapid, portable UA monitoring in healthcare and clinical diagnostics.
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产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Quanliang Li,Xiaomeng Wang,Jing Ren,Ping Chen,Dayong Ren
Rapid and reliable quantification of meat freshness is crucial for consumer health and food safety. In this study, two simple and portable photosensitive colorimetric cards were fabricated for rapid on-site detection of meat freshness. The colorimetric cards were prepared by dispersing Rose Bengal (RB) into sodium acetate-acetic acid and sodium citrate-citric acid buffer systems containing 3,3′,5,5′-tetramethylbenzidine (TMB) and fixed on filter paper, respectively. The hue of the colorimetric cards was intimately correlated with the amount of volatile base nitrogen absorbed from meat, by which the quality of the meat can be visualized qualitatively. Correlation analysis of total volatile basic nitrogen (TVB-N), red: blue ratio (R/B), total color difference (ΔE), and response sensitivity (S%) demonstrated the accuracy of the proposed colorimetric cards. Principal component analysis (PCA) revealed that the first two principal components of the photosensitive colorimetric cards explained 95.4 %, 93.2 %, 92.9 %, and 87.8 % of the variance of sodium acetate-acetate colorimetric card, and 73 %, 87.9 %, 87.1 %, and 92 % of sodium citrate-citric card, respectively, which allowed for a clear differentiation of meat freshness including freshness, sub-freshness, and spoilage. The prepared photosensitive colorimetric cards can accurately reflect meat quality within 4 min. The proposed colorimetric cards are expected to be a prospective universal platform for on-site accurate quantification of meat freshness.
研究领域:
食品安全
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分析化学
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传感器
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比色检测
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光化学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF KING SAUD UNIVERSITY SCIENCE
·
2019
·
IF 2.835
Shuai Zhang,Shoulian Wei,Hao Cheng,Binquan Rong
In this paper, DMC was prepared via carbonization in nitrogen with plexiglass as the template and sucrose as the carbon source. The hierarchical porous carbon (DHPC) was prepared by treating DMC with potassium hydroxide. The DHPC was loaded on chitosan to prepare DHPC@CS, which was then doped into nanosilver via the one-pot process to prepare DHPC@CS-AgNPs. The morphology of DHPC@CS-AgNPs was characterized using the Scanning Electron Microscope (SEM) and the Transmission Electron Microscope (TEM). In the presence of Hg 2+ , DHPC@CS-AgNPs will catalyze the oxidation of TMB, resulting in an observable change of color, so a rapid, sensitive and highly selective method could be established to detect trace Hg 2+ colorimetric sensors. The conditions of the TMB color reaction, characteristics of the Hg 2+ -DHPC@CS-AgNPs oxidized enzyme mimics and their color reaction characteristics were studied. Under the optimized conditions, coloration could not be catalyzed in the DHPC@CS-AgNPs-TMB system, but with the addition of the mercury ions, the catalytic activity was enhanced and the oxidized enzyme mimic activity was observed. In the Hg 2+ -DHPC@CS-AgNPs-TMB system, when TMB was used as a substrate, K m = 0.0165 mM, and V max = 4.6512 × 10 −8 M·s −1 . Under the optimized conditions, the linear range of mercury ion was determined to be 1 × 10 −8 –1 × 10 −6 mol·L −1 using the above method, the linear relationship was r = 0.99753, the detection limit (S/N = 3) was 7 × 10 −9 mol·L −1 , and the recoveries of spiked water sample and fish sample ranged from 84.4% to 103%.
研究领域:
分析化学
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环境科学
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材料科学
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纳米技术
✓ 已验证
产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2016
·
IF 7.476
Shurong Tang,Meili Wang,Zhijun Li,Ping Tong,Qiang Chen,Guangwen Li,Jinghua Chen,Lan Zhang
This work demonstrates the use of quantum dots (QDs) with photocatalytic activity as a sensitive, inexpensive and rapid colorimetric platform for Cu 2+ sensing. Based on the simple thiol compound mediated QDs growing method, CdS QDs can be quickly formed in situ, which possess excellent photocatalytic ability for the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to produce a colored product under light irradiation . Cu 2+ can catalyze the oxidation of sulfhydryl groups in glutathione (GSH) which act as a stabilizer for CdS QDs. In the presence of Cu 2+ , GSH is oxidized and loss the stabilization ability for the growth of CdS QDs, thus resulting in the decrease of the absorbance. Under optimum conditions, as low as 5.3 nM Cu 2+ can be detected. This sensing system is simple, reliable and holds great potential to provide a new general platform for ultrasensitive monitoring of a variety of analytes .
研究领域:
化学
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材料科学
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分析化学
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环境科学
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产品关联
DOI
PubMed
高影响因子
ANALYST
·
2020
·Vol. 145
·期 19
·6349-6356
·
IF 3.978
Ye Hu,Chaochao Yue,Jing Wang,Yuhua Zhang,Wenhui Fang,Jiaqi Dang,Ying Wu,Hong Zhao,Zengxi Li
Fe–Ni-MOFs with different amounts of Fe are synthesized through a two-step template etching method. Kinetic analysis indicates that Fe–Ni-MOF exhibits prominent intrinsic peroxidase-like activity, which could catalytically oxidize 3,3′,5,5′-tetramethylbenzidine (TMB) to produce oxidized TMB (oxTMB) with a blue color. As a peroxidase-mimicking MOF, Fe–Ni-MOF with its efficient stable catalysis properties is demonstrated to allow a sensitive colorimetric assay for Sn2+ ions. The mechanism is explored, whereby Sn2+ ions could reduce the peroxidase-like activity of Fe–Ni-MOF based on a redox interaction, making the oxTMB color lighter and decreasing the absorbance intensity at 652 nm. The linear determination of the Sn2+ ion concentration using a UV-vis spectrometer ranges from 0.01 mM to 1.0 mM and from 1.0 mM to 4.0 mM. The presented Fe–Ni-MOF-based assay of Sn2+ ions was successfully applied to real water samples.
研究领域:
材料科学
·
分析化学
·
环境科学
✓ 已验证
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2017
·
IF 4.95
Cuiying Lin,Yajuan Guo,Mengmeng Zhao,Mi Sun,Fang Luo,Longhua Guo,Bin Qiu,Zhenyu Lin,Guonan Chen
Development of simple but sensitive biosensor for influenza detection is highly important in immediate and effective clinical treatment. In this study, a sensitive colorimetric immunosensor which combines the advantages of high selectivity of immunoassay and simplicity of colorimetric detection has been developed to detect influenza virus H5N1 based on enzyme-encapsulated liposome. Biotin-tagged liposome encapsulated with large amount of horseradish peroxidase (HRP) was firstly synthesized. In the presence of H5N1, H5N1 co-bound with the capture antibody and the biotinylated detection antibody to form sandwich immunocomplex . Subsequently, the HRP-encapsulated liposome was introduced to conjugate with the detection antibody through biotin-avidin-biotin linkage. Upon the addition of substrate (mixture of 3,3′,5,5′-tetramethylbenzidine (TMB) and H 2 O 2 ), the liposome was directly lysed to release large amount of HRP by TMB. The released HRP catalyzed the H 2 O 2 -mediated oxidation of TMB, resulting in color change of the system, which was observed by naked eyes or UV–vis spectra. The result showed that the absorption intensity enhanced with the increase of H5N1 concentration ranging from 0.1 to 4.0 ng/mL, and the detection limit was calculated to be 0.04 ng/mL. The sensitivity of the proposed biosensor is much higher than that of conventional enzyme-linked immunosorbent assay method. The proposed immunosensor is relatively simple, low-cost, sensitive, and selective without using any sophisticated instruments, therefore it may have a promising prospect for detecting targets in clinical medicine, food safety analysis, and environmental monitoring.
研究领域:
生物技术
·
病毒学
·
分析化学
·
医学诊断
✓ 已验证
DOI
PubMed
高影响因子
TALANTA
·
2013
·
IF 3.498
Rui Zhu,Yan Zhou,Xi-Liang Wang,Li-Ping Liang,Yi-Juan Long,Qin-Long Wang,Hai-Jie Zhang,Xiao-Xiao Huang,Hu-Zhi Zheng
It was found that Hg 2+ can inhibit the peroxidase mimetic activity of bovine serum albumin (BSA) protected Au clusters (BSA-Au) due to the specific interaction between Hg 2+ and Au + existed onto the surface of BSA-Au clusters. By coupling with 3, 3′, 5, 5′-tetramethylbenzidine (TMB)–H 2 O 2 chromogenic reaction, a novel method for Hg 2+ detection was developed based on the inhibiting effect of Hg 2+ on BSA-Au clusters peroxidase-like activity. This method exhibited high selectivity and sensitivity. As low as 3 nM (0.6 ppb, 3 σ ) Hg 2+ could be detected with a linear range from 10 nM (2 ppb) to 10 µM (2 ppm) and this method was successfully applied for the determination of total mercury content in skin lightening products.
研究领域:
分析化学
·
生物化学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）