Journal of Inflammation Research
·
2025
·
IF 4.1
Jiaxin Di,Meifang Guo,Maijiao Peng,Leyao Fang,Junxi Shen,Nenqun Xiao,Zhoujin Tan
Purpose To investigate the therapeutic mechanisms of sodium butyrate combined with SSP in treating diarrhea with kidney-yang deficiency syndrome, providing experimental evidence for the modern application of classical Chinese medicine formulas.Methods A mouse model of diarrhea with kidney-yang deficiency syndrome was established via adenine combined with Folium sennae gavage. After successful model establishment, the drug intervention groups were administered by gavage with 100% SSP decoction, 300 mg/mL sodium butyrate, and 100 mg/kg sodium butyrate + 50% SSP decoction, respectively. General physical conditions and organ indices were assessed. Serum tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) levels, as well as colonic immunoglobulin A (sIgA) and mucin 2 (MUC2) levels, were determined via ELISA. Kidney and intestinal histology were evaluated via hematoxylin‒eosin (HE) staining. The characteristics of the small intestinal mucosal microbiota under different interventions were analysed via 16S rRNA sequencing.Results Compared with natural recovery, the combination of 100 mg/kg sodium butyrate + 50% SSP significantly restored colonic sIgA levels (p< 0.05) and MUC2 levels, indicating an improvement in intestinal mucosal barrier function. Histological analysis revealed that the combination of 100 mg/kg sodium butyrate + 50% SSP improved renal inflammation and restored intestinal villus length and crypt depth. The combination therapy controlled the relative abundance of Corynebacterium while restoring Dwaynesavagella and Megasphaera_A. Correlation analysis indicated that Mammaliicoccus and Corynebacterium were significantly negatively correlated with sIgA and MUC2 levels and that their relative abundances were inhibited by the combination therapy.Conclusion Sodium butyrate enhanced the therapeutic efficacy of SSP. The combination therapy effectively repaired damaged kidneys and intestines and alleviated diarrhea with kidney-Yang deficiency syndrome by modulating the relative abundance of intestinal microbiota and enhancing intestinal immunity as well as mucosal barrier function. The interaction between the intestinal microbiota and intestinal mucosal protective factors may be a key mechanism in the combined treatment of diarrhea with kidney-yang deficiency syndrome.
研究领域:
药理学
·
中医学
·
微生物学
·
免疫学
·
胃肠病学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Xiaowei Zhang,Yutao Zhang,Zhou Xu,Wenmeng Liu,Boyan Gao,Jianhua Xie,Tingting Chen,Enpeng Li,Baoguo Li,Cheng Li
Crosslinked corn bran arabinoxylan (CLAX) is a food hydrocolloid that can be applied to improve the physicochemical and digestion properties of starch. However, the impact of CLAX with different gelling characteristics on starch properties remains elusive. Here, high cross-linked arabinoxylan (H-CLAX), moderate crosslinked arabinoxylan (M-CLAX), and low crosslinked arabinoxylan (L-CLAX) were fabricated to investigate their effects on the pasting, rheological, structural, and in vitro digestion property of corn starch (CS). The results showed that H-CLAX, M-CLAX, and L-CLAX differently increased the pasting viscosity and gel elasticity of CS, with H-CLAX exhibiting the greatest effect. The structural characterization of CS-CLAX mixtures showed that H-CLAX, M-CLAX, and L-CLAX differently enhanced the swelling power of CS and increased the hydrogen bonds between CS and CLAX. Furthermore, the addition of CLAX (especially H-CLAX) significantly reduced both the digestion rate and extent of CS, probably due to the increased viscosity and the formation of the amylose-polyphenol complex. This study provided new insights into the interaction between CS and CLAX, and could help to develop healthier foods with slow starch digestibility.
研究领域:
食品科学
·
生物化学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
·
2023
·
IF 5
Xun Wang,Like Ning,Hanchen Lin,Ning Ma,Xun Li,Fei Wang,Rui Zhang,Chaoqun You
The study of mitochondria-targeted antitumor treatment has been extensively developed as target sites to improve the efficiency of cancer therapy. On this basis, cellulose nanocrystal (CNC) with large specific surface areas, which can conjugate large amounts of active groups, have been provided with the potential antitumor platform for developing mitochondria-targeted triggers. Herein, an efficient mitochondria-targeting nanocellulose composite hydrogel based on triphenylphosphine (TPP) conjugated quaternized cellulose nanocrystal (QCNC) and agarose was developed and used as mitochondria targeting triggers to deliver drugs to cancer cells. Furthermore, TPP conjugated dihydroartemisinin (DHA) was loaded into the nanocellulose composite hydrogels, accompanied by the significant generation of reactive oxygen species (ROS), and resulted in cell apoptosis. This DHA-loaded nanocellulose composite hydrogel has achieved a doubled ROS yield than free DHA, and the results showed that the viability of the A549 cell was reduced by 50 % by nanocellulose composite hydrogels treatment compared to free DHA. Moreover, the nanocellulose composite hydrogel demonstrated nontoxic performance in vitro cytotoxicity studies on HUVEC cells. This current study indicates the strong potential for using nanocellulose as biocompatible and efficient mitochondria-targeted triggers in cancer therapies.
研究领域:
生物医学工程
·
肿瘤学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
Materials Today Bio
·
2023
·
IF 8.2
Zhaowei Yin,Chaoren Qin,Shaowei Pan,Chen Shi,Guanfu Wu,Yan Feng,Jing Zhang,Ziyi Yu,Bin Liang,Jianchao Gui
Exosomes (Exos) secreted by adipose-derived stem cells (ADSCs) have shown potential in alleviating osteoarthritis (OA). Previous studies indicated that infrapatellar fat pad (IPFP) derived stem cells (IPFSCs) may be more suitable for the treatment of OA than subcutaneous adipose tissue (ScAT) derived stem cells (ScASCs). However, it remains unclear which type of Exos offers superior therapeutic benefit for OA. This study first compared the differences between Exos derived from IPFP stem cells (Exos IPFP ) and ScAT stem cells (Exos ScAT ) in OA treatment. Results suggested that Exos IPFP significantly inhibit the degradation of cartilage extracellular matrix (ECM) than Exos ScAT , following this, the differences in microRNA (miRNA) expression between the two types of Exos using small RNA sequencing were performed. Subsequently, miR-99 b-3p was chosen and over-expressed in Exos ScAT (Exos ScAT−99b−3p ), both in vivo and in vitro experiments demonstrated its efficacy in inhibiting the expression of ADAMTS4, promoting the repair of the ECM in OA. Finally, microfluidic technology was performed to fabricate a hyaluronan-based hydrogel microparticles (HMPs) for encapsulating Exos (HMPs@exos), the injectability, sustained release of Exos and long-term therapeutic effect on OA were validated. In summary, these results suggest miR-99 b-3p regulates the degradation of cartilage ECM by targeting ADAMTS4, the upregulation of miR-99 b-3p in Exos ScAT would enable them to exhibit comparable or even superior effectiveness to Exos IPFP for OA treatment, making it a promising approach for OA treatment. Considering the abundant resources of ScAT and the limited availability of IPFP, ScAT harvested through liposuction could be genetically engineered to yield Exos for OA treatment. Furthermore, the encapsulation of Exos in HMPs provides an injectable sustained local drug release system, which could potentially enhance the efficacy of Exos and hold potential as future therapeutic strategies.
研究领域:
生物材料
·
干细胞治疗
·
基因治疗
·
骨科
·
药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Environmental Sciences
·
2020
·
IF 4.302
Xiaoyang Fan,Yili Wang,Daxin Zhang,Yajie Guo,Shihui Gao,Enrui Li,Huaili Zheng
The effects of four conditioning approaches: Acid, Acid-zero-valent iron (ZVI)/peroxydisulfate (PMS), Fe(II)/PMS and ZVI/PMS, on wastewater activated sludge (WAS) dewatering and organics distribution in supernatant and extracellular polymeric substances (EPS) layers were investigated. The highest reduction in bound water and the most WAS destruction was achieved by Acid-ZVI/PMS, and the optimum conditions were pH 3, ZVI dosage 0.15 g/g dry solid (DS), oxone dosage 0.07 g/g DS and reaction time 10.6 min with the reductions in capillary suction time (CST) and water content (Wc) as 19.67% and 8.49%, respectively. Four conditioning approaches could result in TOC increase in EPS layers and supernatant, and protein (PN) content in tightly bound EPS (TB-EPS). After conditioning, organics in EPS layers could migrate to supernatant. Polysaccharide (PS) was easier to migrate to supernatant than PN. In addition, Acid, Acid-ZVI/PMS or Fe(II)/PMS conditioning promoted the release of some polysaccharides containing ring vibrations v P O, v C-O-C, v C-O-P functional groups from TB-EPS. ESR spectra proved that both radicals of SO 4 - · and ·OH contributed to dewatering and organics transformation and migration. CST value of WAS positively correlated with the ratios of PN/PS in LB-EPS and total EPS, while it negatively correlated with TOC, PN content and PS content in TB-EPS, as well as PS content in supernatant and LB-EPS. BWC negatively correlated to zeta potential and TOC value, PN content, and HA content in supernatant.
研究领域:
环境科学
·
污水处理
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Cultural Studies of Science Education
·
2021
·Vol. 16
·期 1
·47-69
·
IF 1.167
Rivera Seema,Oliveira Alandeom
The present study examines the pedagogical use of reading aloud of science trade books as an effective tool for teaching nature of science (NOS) to elementary students. To this end, we explore elementary teachers’ and students’ dialogic negotiation of NOS during interactive science read-alouds, as well as potential interactions between their sense-making patterns, NOS views, and trade-book representations of NOS. It was found that, when a book had explicit NOS aspects in it, a teacher with more informed NOS views was able to facilitate a more extended, open-ended, and inclusive discussion about NOS. Conversely, when the trade book had very explicit connections, a teacher with naïve NOS views was able to only superficially address these NOS aspects without going beyond or elaborating on the information available in the book. Furthermore, the latter discussion was characteristically close-ended, exclusive of students, and limited in sense-making. These findings underscore the need for further investigation of how particular NOS aspects are narrativized in science trade-books, and how elementary teachers can effectively guide students while facilitating explicit negotiation of particular types of trade book representations of NOS during interactive science read-alouds. It is argued that improving elementary science instruction requires a more sophisticated, theory-based understanding of how NOS instruction is mediated by stories and storytelling.
研究领域:
科学教育
·
小学教育
✓ 已验证
DOI
高影响因子
LANGMUIR
·
2023
·
IF 3.9
Donghai Sheng,Lin Zhang,Hongfei Shang,Baoming Guo,Yuan Li
The contact between the blood and the surface of medical materials causes a series of rejection reactions. In this process, the plasma protein is adsorbed to the surface of materials within seconds and binds to glycoprotein receptors on platelets, causing platelet activation, coagulation cascade, and complement activation to form thrombus, which greatly limits the application of medical materials. In our work, the surface of poly(4-methyl-1-pentene) hollow fiber membranes (PMP HFMs) was coated with a diamond-like carbon (DLC) film by the ion plating method. The blood compatibility of the DLC coating was evaluated by protein adsorption, platelet adhesion, clotting time, red blood cell (RBCs) hemolysis, dynamic coagulation, and extracorporeal blood circulation tests. Compared with the unmodified PMP membrane, the DLC film could effectively reduce protein adsorption and platelet adhesion and prolong the coagulation time. The DLC coating showed BSA adsorption of as low as 0.53 μg/cm2 as well as a long activated partial thromboplastin time (APTT) value of 71.84 s. Furthermore, the PMP membrane modified with the DLC coating was used for extracorporeal blood circulation without thrombosis forming within 28 days. The DLC coating is one of the most promising medical coatings as an artificial lung membrane in extracorporeal membrane oxygenation (ECMO) equipment.
研究领域:
生物医学工程
·
材料科学
·
血液学
✓ 已验证
DOI
高影响因子
Food Chemistry: Molecular Sciences
·
2026
·
IF 4.8
Yongbang Si,Yongna Song,Dong Huang,Yifei Chen,Jun Sun,Zezhong Wu,Rui Ma,Guoliang Sun,Yuqiong Meng
Driven by sustainability, plant oils are increasingly used to replace fish oil in aquafeeds, thereby altering muscle fatty acid composition and potentially affecting volatile profiles. Lipoxygenases (LOXs) participate in fatty-acid bioconversion, but the role of 5-lipoxygenase (5-LOX) in non-immune fish muscle remains unclear. We hypothesized that acute intragastric exposure to plant-derived C18 fatty acids would be associated with changes in muscle 5-LOX protein abundance and volatile profiles in triploid rainbow trout. Using an acute oral gavage model, trout received oleic, linoleic, or α-linolenic acid, alone or with the selective 5-LOX inhibitor zileuton. Muscle samples were analyzed for 5-LOX mRNA expression, detectable protein abundance, and volatile profiles. Detectable 5-LOX protein abundance was low under basal conditions and changed after fatty-acid administration, with linoleic acid showing the clearest increase. Several major volatiles also changed across treatments. Under acute fatty-acid exposure, zileuton reduced hexanal concentration by 85.7% (221.2 to 31.6 ng/g) and decreased the nonanal odor activity value by 34.4%. These results indicate that acute plant-derived C18 fatty acid exposure was associated with changes in detectable muscle 5-LOX protein abundance and volatile profiles in triploid rainbow trout. The inhibitor-associated volatile changes further support the possibility that multiple oxidative processes may be involved under these acute conditions.
✓ 已验证
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Bo Hu,Wei Wei,Fengxia Liu,Xiaofei Xu,Zhiyi Li,Zhijun Liu
To achieve superior regeneration properties of the CO 2 -rich phase without additional auxiliary measures in biphasic CO 2 absorbents (e.g., N 2 purging or co-activators), a dual-functionalized ionic liquid biphasic solvent of N-Aminoethylpiperazine-Lysine/N-Propanol/water ([AEPH][Lys]/NPA/H 2 O) was designed for CO 2 capture. It demonstrates a remarkable CO 2 loading of 1.847 mol·mol −1 , while the rich volume being only 53.0 % of the total absorbent and the viscosity as low as 6.10 mPa·s. Remarkably, 87.26 % regeneration efficiency was achieved under 393.15 K for 40 min without additives, and the regenerated lower phase could homogeneously recombine with the upper phase. After 10 cycles, regeneration efficiency remained at 84.67 %, while energy consumption was only 1.90 GJ·ton -1 CO 2 (49.87 % lower than MEA). 13 C NMR revealed that bicarbonate/carbonate dominated the CO 2 products, as well as some carbamates and a small amount of propyl carbonates. The reaction pathways of bicarbonates/carbonate based on quantum chemical calculations were indicated that through base-catalyzed hydration of tertiary amines on AEPH + cations, hydrolysis of carbamates and the reaction of water with CO 2 . The bicarbonate/carbonate exhibits lower thermal stability than carbamates, facilitating efficient regeneration. Based on MPI index, solvation-free energy and weak intermolecular interactions analysis, the solubility of CO 2 products in water is higher than NPA. This absorbent demonstrates significant potential for industrial CO 2 capture applications.
研究领域:
化学工程
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Qiao Feng Wang,Ming Sun,Jiang Bing Sun,Jun Zhou
A novel and efficient method of surfactant-assisted temperature-controlled solidification ionic liquid dispersion liquid–liquid microextraction (SA-TCS-IL-DLLME) has been developed for the preconcentration and determination of trace five phthalate esters in bottled water. The analysis was completed by ultra-high performance liquid chromatography (UHPLC). In this study, hydrophobic low-density solid quaternary phosphonate ionic liquid ([P 44412 ] BF 4 ) was used as extractant while surfactant Tergitol TMN-6 was employed as dispersant. The dispersion of the extractant in the sample solution was promoted by water bath and vortex. At the same time, the temperature was controlled to transform the extractant from liquid to solid. Then, the separation of the extractant and the sample solution was achieved. Incidentally, extraction of the analyte was also finished. Important parameters which affected SA-TCS-IL-DLLME efficiency, such as the type and volume of extractant and dispersant, the amount of salt, the vortex time and pH value were investigated and optimized by single factor experiments. Under these optimized conditions, the method exhibited linearity ranges of 0.01–1.0 ng mL −1 with correlation coefficients between 0.9994 and 0.9998. The method’s intra-day ( n = 6) and inter-day ( n = 6) precision levels were maintained at ≤ 3.37 %, which was considered to be within acceptable limits. The computed detection and quantification limits were 16.54–24.60 and 54.91–82.04 ng mL −1 , respectively. Above all, this optimized method was effectively employed to extract five phthalate esters from bottled water with recoveries ranging from 94.83 % to 98.72 %. It can be used for rapid analysis and detection of phthalate esters in bottled water samples.
研究领域:
分析化学
·
环境科学
·
食品科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
·
2019
·
IF 2.606
Jiahui He,Wei Wang,Huicong Zhou,Pan She,Pantian Zhang,Yi Cao,Xuefei Zhang
A novel cytocompatible and pH-sensitive amphiphilic polymeric prodrug named as mPEG- b -norbornene functional PLA-graft-Doxorubicin (mPEG- b -PLA- g -DOX) was synthesized by ROP (ring-opening polymerization), then condensation and followed by click reaction. The polymeric carriers were grafted with triazo group. Doxorubicin (DOX) was modified by the cyclooctyne via condensation reaction and conjugated to the polymeric carriers through the SPAAC (Strain-Promoted Alkyne-Azide Cycloaddition) click reaction. There was a Schiff base between the DOX and cyclooctyne which could result in the pH stimuli-responsiveness for controlled release of anti-cancer drug. The resultant mPEG- b -PLA- g -DOX prodrug was characterized by the 1 H NMR, GPC and HPLC. The content of DOX in prodrug reached 17.4% as measured by the UV spectrum. The amphiphilic polymeric prodrug could self-assemble into micelle which was characterized by the dynamic light scattering (DLS) and scanning electron microscope (SEM). The profile of in vitro drug release exhibited enhanced drug release at acidic pH (pH = 5.3) compared with neutral pH (pH = 7.4). The results of MTT assay showed that the amount of DOX required for MCF-7 cells was reduced after DOX molecules were prepared into polymer prodrug, which was conducive to reducing side effects under the premise of ensuring inhibitory effect.
研究领域:
药学
·
肿瘤学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
Journal of Cleaner Production
·
2022
·
IF 11.072
Shiyu Fang,Wangbei Cao,Qianqi Shao,Wenxuan Huang,Feng Wang,Xiaoshi Cheng,Jiashun Cao,Jingyang Luo,Yang Wu
Crawfish shell wastes (CSW) are abundant with valuable substrates, which possess great resource potentials. This work has revealed the feasibility of CSW reutilization for effective volatile fatty acids (VFAs) generation with waste active sludge (WAS) via anaerobic fermentation. The maximum VFAs generation reached 17963 mg COD/L when the CSW/WAS mass ratio was 3.25:1, while it was 784 mg COD/L in the control reactor. Meanwhile, each gram of CSW could be transformed into 264 mg COD equivalent VFAs. The feed of CSW brought in massive organic substrates, which significantly promoted the solubilization , hydrolysis and acidification processes in the CSW/WAS co-fermentation systems simultaneously. The metabolism of carbohydrates took priority over proteins in contributing to the VFAs promotion. More importantly, the functional anaerobes ( i.e. , Paludicola sp. and Macellibacteroides sp.), which participated in the main substrates decomposition with the VFAs as the primary metabolites , were evidently enriched in the presence of CSW. The critical hydrolases and acid-forming enzymes relevant to VFAs biosynthesis were all enhanced. Moreover, the key genes encoding for the main substrates metabolisms ( i.e., chitin) and VFAs production were remarkably up-regulated. The evident promotion of microbial activities and metabolic functions was mainly ascribed to the favorable fermentation conditions ( i.e., pH, ORP and C/N) induced by CSW conditioning. This study proposed an innovative strategy to recover high-value products from CSW, and in-depth insights of microbial traits and genetic functions in CSW/WAS co-fermentation systems.
研究领域:
环境工程
·
生物技术
·
微生物学
·
废物管理
·
生物化学工程
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）