ENVIRONMENTAL RESEARCH
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2024
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IF 7.7
Xingyao Ye,Xiaojun Niu,Ling Li,Mengyu Lv,Dongqing Zhang,Deye Chen,Yu Line,Zhiquan Yang
N-(1,3-Dimethylbutyl)-N′-phenyl-p-phenylenediamine (6PPD) is an antioxidant commonly used in tire manufacturing, and its release into the environment has significantly increased due to rapid urbanization. When subjected to ozonation, 6PPD converts into the harmful pollutant 6PPD quinone (6PPDQ). These substances enter wastewater treatment plants (WWTPs) via stormwater runoff and pipelines, posing significant risks to the functional microorganisms. Anammox, a strictly controlled and sensitive microbial nitrogen removal process, is especially susceptible to the effects of the pollutants. This study investigates the comprehensive impact of 6PPD-Q and 6PPD on anammox communities based on characterization analysis and metagenomics. At environmental concentrations, 6PPD-Q at 200 ng/L-1000 ng/L led to the disintegration of anammox granules. Extended exposure to both 6PPD-Q and 6PPD significantly reduces the population of anammox bacteria (AnAOB). By utilizing organic matter from dead cells and incoming carbonate as a carbon source, the system evolved into a nitrogen metabolism network primarily focused on denitrification and dissimilatory nitrate reduction to ammonium (DNRA). This transformation was accompanied by a reshuffling of the microbial community and associated genes, resulting in an accumulation of NH 4 + -N. These findings underscore the toxicity of 6PPD-Q and 6PPD to anammox and stress the importance of incorporating 6PPD into regulatory and preventive strategies.
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PubMed
高影响因子
Journal of Environmental Chemical Engineering
·
2025
·Vol. 14
·期 1
·120710
·
IF 7.2
Zepeng Wang,Qing Xia,Zhangliang Li,Tao Liu
The application of anammox process is often constrained by the slow growth rate and low bioactivity of anammox bacteria (AnAOB). While zero-valent iron (ZVI) supplementation is a recognized strategy to enhance anammox, its direct addition poses operational challenges. This study proposed a novel approach by developing suspended carriers with melt-blended ZVI to provide sustained iron release and physical support for biofilm growth. Three types of ZVI5@HDPE, ZVI10@HDPE and ZVI15@HDPE carriers were prepared with varying ZVI loadings (5, 10 and 15 wt% respectively) using high-density polyethylene (HDPE), and evaluated in the anammox systems over 87 days. Results demonstrated that ZVI modification significantly enhanced carrier surface hydrophilicity (static water contact angle was 92.55 ± 2.33° for HDPE carriers and 77.76 ± 2.74° for ZVI15@HDPE carriers) and roughness (arithmetical mean deviation of microscopic profile was 37.0 ± 2.4 nm for HDPE carriers and 70.3 ± 4.2 nm for ZVI15@HDPE carriers), which facilitated microbial attachment and biofilm development. Moreover, these ZVI-modified carriers promoted the specific anammox activity (0.13 ± 0.06 g N/(gVSS·d) for HDPE carriers and 0.20 ± 0.01 g N/(gVSS·d) for ZVI15@HDPE carriers), increased heme c content, and enhanced key anammox enzymes activity. Nitrogen removal performance was superior in reactors with ZVI-modified carriers, with ZVI15@HDPE system achieving over 80 % total nitrogen removal efficiency. Microbial community analysis revealed a selective enrichment of Candidatus_Kuenenia (70 % relative abundance) on ZVI15@HDPE carriers, alongside an upregulation of functional genes related to energy metabolism and biofilm formation. This study confirms that ZVI-blended suspended carriers are an effective strategy to augment AnAOB enrichment and bioactivity, overcoming the limitations of direct ZVI supplementation.
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高影响因子
Journal of Cleaner Production
·
2024
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IF 9.7
Jia-Min Zhou,Ying-Jun Sun,Zhi-Han Xi,Zu-Gang Liao,Xin Ma,Ze-Tong Feng,Xin-Tao Liu,Xin-Rui Zhang,Ying Wang,Ying Chen,Ren-Cun Jin,Qian-Qian Zhang
The implications of zero-valent iron (ZVI) on performance of partial denitrification (PD) coupled with anammox process (PD-anammox) and microbial community structures were assessed in present investigation. The results indicated that removal efficiencies of nitrate nitrogen, ammonium nitrogen and total nitrogen (TN) reached 86.28 ± 0.04%, 81.05 ± 0.06% and 82.90 ± 0.80%, respectively, with 30-day addition of ZVI under ratio of ZVI to nitrate (ZVI/N), carbon to nitrate (C/N) respectively for 3 and 2.3. Moreover, the content of extracellular polymer substance elevated by ZVI, and which could substitute carbon sources, mediated electron transfer and promoted nitrogen removal efficiency. Additionally, microbiological analysis revealed that ZVI enhanced nitrogen removal process by promoting the growth of genus Ignavibacterium and Denitratisoma , which coexist with anaerobic ammonia oxidation bacterium (AnAOB) and also induced other nitrogen removal process such as heterotrophic nitrate reduction to ammonium (DNRA), nitrate-dependent anaerobic ferrous oxide (NAFO), and iron-based anammox (Feammox) process. This study offers new insights into the enhancement of the nitrogen removal process by ZVI in PD-anammox process.
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高影响因子
BIORESOURCE TECHNOLOGY
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2018
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IF 5.807
Zheng-Zhe Zhang,Ya-Fei Cheng,Lian-Zeng-Ji Xu,Yu-Hui Bai,Jia-Jia Xu,Zhi-Jian Shi,Yang-Yang Shen,Ren-Cun Jin
The increasing use of engineered metal oxide nanoparticles (MONPs) in consumer products raises great concerns about their environmental impacts, but their potential impacts on anaerobic ammonium oxidation (anammox) process in wastewater treatment remain unclear. In this study, the presence of MONPs (1, 50, 200 mg L −1 ) exhibited no visible effects on the nitrogen removal performance of anammox reactors, but high levels (200 mg L −1 ) of SiO 2 NPs, Al 2 O 3 NPs and CeO 2 NPs had a distinct effect on shaping the anammox community. Long-term exposure of MONPs caused different responses in the relative abundance of Ca. Kuenenia , the level of functional gene Hzs A and the activities of three key enzymes involved in anammox metabolism, but no significant inhibition effects on specific anammox activity were detected. Overall, the effects of MONPs on anammox community structure and sludge properties depended on their types and levels and followed the order SiO 2 > CeO 2 > Al 2 O 3 > TiO 2 .
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PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2024
·
IF 9.7
Zongshuo Han,Ruijie Hu,Xiaoying Zheng,Zhilin Zhao,Wenfei Li,Haidong He,Tao Lin,Hang Xu
The long acclimation period and sensitivity to environmental conditions of Anammox are the bottlenecks for its promotion and application. An innovative strategy was adopted to accelerate functional microbial enhancement and improve nitrogen removal performance by inoculating cryopreserved Anammox sludge and activated sludge with intermittent dosing of nanoscale zero-valent iron (nZVI). The acclimation time was shortened by 76 days with nitrogen removal efficiency (NRE) reaching up to 91.07 %. Anammox, NDFO (nitrate/nitrite-dependent Fe(II) oxidation), Feammox (Fe(III) reduction coupled with anaerobic ammonium oxidation) and abiotic reactions were coupled in the system with nZVI, contributing to 69.79 %, 15.14 %, 9.84 % and 0.25 % of nitrogen removal, respectively. Further microbial analysis demonstrated significant enrichment of functional microorganisms, such as Candidatus Jettenia , Acidovorax and Comamonas . High-efficient nitrogen removal was attribute to the increase of functional genes involved in Anammox, electronic transfer, heme C synthesis and iron metabolism. This work provides an inspiring idea for the mainstream Anammox application.
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PubMed
高影响因子
Journal of Environmental Chemical Engineering
·
2024
·
IF 7.4
Lingjie Liu,Shaoqing Mo,Shaopo Wang,Jing Chang,Jianhui Zhao,Jingjie Yu
Low temperatures were a critical factor that inhibited anaerobic ammonium oxidizing bacteria (AnAOB) activity. This study demonstrated the effect of exogenous zero-valent iron (ZVI) on Anammox-hydroxyapatite (HAP) system at 17°C. The results showed that long-term addition of ZVI increased the system nitrogen removal efficiency by 8.59% and alleviated the inhibition of AnAOB by low temperature. After addition of ZVI, the activity of AnAOB and the heme c content increased by 58.20% and 4.83%, respectively. Moreover, the functional enzyme activities of NIR, HZS, and HZO increased by 94.12%, 36.36%, and 16.67%, respectively. The concentrations of quorum sensing molecules, i.e., C 6 -HSL and C 8 -HSL, increased by 191.17% and 41.54%, respectively, after ZVI addition. Metagenomic analysis revealed that the addition of 5 g/L ZVI stimulated the relative abundance relative abundance of Fe(II) metabolism-related genes in AnAOB at 17 °C, thereby contributing to the uptake, transport, utilization, and storage of Fe(II). The addition of ZVI could improve the nitrogen removal performance of the anammox-HAP system at the low temperature.
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高影响因子
Journal of Water Process Engineering
·
2024
·
IF 6.3
Yunlong Yang,Pan Yang,Minjie Li,Jinkui Zhang,Xulin Chen,Heng Meng,Hui Zheng
Spent mushroom substrates (SMSs) were recycled as solid-liquid mixed carbon sources in a single chamber microbial fuel cell (SC-MFC) to investigate nitrogen removal and electricity generation performances as well as explore the corresponding mechanisms. The leaching experiments showed that the release rate of COD was as high as 59.75 mg/(g·d) for the first stage but it declined sharply to 0.3 mg/(g·d) for the second stage, and denitrification performance relied on COD released from SMS at the beginning. A pH of 6.5, a solid-liquid ratio of 1:100 and an electrode distance of 3 cm were deemed as the optimum operating conditions of SC-MFC. For the long-term operation of SC-MFC, the total nitrogen removal efficiency exceeded 50 % and the average voltage output attained 153 mV. Microbial diversity indicated that the anode enriched hydrolyzing bacteria, fermentation bacteria and electricigens, whereas the cathode was found to be predominated by denitrifying bacteria and anammox microorganisms, suggesting nitrate was mainly removed in the cathodic area. The membrane-less structure and SMS as substrates mutually created a special environment that induced concurrent denitrification and anammox. Our results can provide useful information for understanding the mechanisms of using SMS as solid-liquid carbon sources for simultaneous nitrogen removal and electricity generation.
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高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2017
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IF 6.216
Xiaojing Zhang,Yue Zhou,Tengfei Xu,Kaiwei Zheng,Rongrong Zhang,Zhaoxue Peng,Hongzhong Zhang
In this study, a sequencing batch reactor (SBR) was operated as Anammox and used as parent reactor. Then three SBRs seeded from the parent reactor were adopted for investigating the effect of CuO, ZnO and TiO 2 nanoparticles (NPs) on nitrogen removal, microbial activity and microbial communities of Anammox. The bioactivities of anaerobic ammonia-oxidizing bacteria (AAOB) after different exposure period were detected by batch experiments. Results showed that NPs performed immediate and durable toxicity on Anammox. The nitrogen removal efficiency decreased to 2.3%, 2.9% and 14.0% from 71%, the Anammox rate decreased to 0.13, 0.16 and 0.75 from 2.76 mg h −1 g −1 VSS, and the relative abundance of AAOB decreased to 2.57%, 2.78% and 2.61% from 7.99%, respectively. CuO and ZnO NPs performed similar effect, while CuO NPs led to the most severe toxicity and lowest accumulative effect. TiO 2 NPs showed the lowest toxicity but most durable suppression on Anammox process.
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高影响因子
BIORESOURCE TECHNOLOGY
·
2017
·
IF 5.651
Zheng-Zhe Zhang,Ya-Fei Cheng,Yu-Hui Bai,Lian-Zeng-Ji Xu,Jia-Jia Xu,Zhi-Jian Shi,Qian-Qian Zhang,Ren-Cun Jin
Magnetic nanoparticles (NPs) have been widely applied in environmental remediation, biomass immobilization and wastewater treatment, but their potential impact on anaerobic ammonium oxidation (anammox) biomass remains unknown. In this study, the short-term and long-term impacts of maghemite NPs (MHNPs) on the flocculent sludge wasted from a high-rate anammox reactor were investigated. Batch assays showed that the presence of MHNPs up to 200 mg L −1 did not affect anammox activity, reactive oxygen species production, or cell membrane integrity. Moreover, long-term addition of 1–200 mg L −1 MHNPs had no adverse effects on reactor performance. Notably, the specific anammox activity, the abundance of hydrazine synthase structural genes and the content of extracellular polymeric substance were increased with elevated MHNP concentrations. Meanwhile, the community structure was shifted to higher abundance of Candidatus Kuenenia indicated by high-throughput sequencing. Therefore, MHNPs could be applied to enhance anammox flocculent sludge due to their favorable biocompatibility.
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高影响因子
CHEMOSPHERE
·
2019
·
IF 5.108
Xingjia Yin,Jing Lu,Yuchun Wang,Guanglong Liu,Yumei Hua,Xiaoqiong Wan,Jianwei Zhao,Duanwei Zhu
Excessive nitrogen has been a global concern to cause lake eutrophication . The denitrification and anammox processes are considered to be effective biological pathways for nitrogen removal. Submerged macrophytes also play a key role in the nitrogen cycle of lakes. However, the mechanism of submerged macrophytes on regulating biological nitrogen removal pathways has not been well quantified. Therefore, this study investigated the impacts of submerged macrophytes on the community structures and abundance of the nirS -type denitrifiers and anammox bacteria in the rhizospheres. The qPCR results indicated that the abundance of two bacteria in the near-rhizospheres of submerged macrophytes was significantly lower than the root compartments and non-rhizospheres, while the concentrations of organic acids in the near-rhizospheres were higher than those of the root compartments and non-rhizospheres. Redundancy analysis results illustrated that concentrations of NO 3 − -N, NO 2 − -N, citric acid and oxalic acid were the key environmental indicators which had the significant impact on the microbial community . The concentrations of citric acid and oxalic acid were negatively correlated with the nirS -type denitrifiers abundance, and the oxalic acid concentrations were negatively correlated with the anammox bacteria abundance. These results indicated that submerged macrophytes could reduce the abundance of nirS -type denitrifiers and anammox bacteria by releasing organic acids. In addition, the highest diversity of denitrifier community were found in the rhizosphere of the Hydrilla verticillata , while the highest diversity of anammox community were found in the Potamogeton maackianus rhizosphere. These results indicate that the impacts of submerged macrophytes on the biological nitrogen removal pathways were species-dependent.
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高影响因子
BIORESOURCE TECHNOLOGY
·
2024
·
IF 9.7
Shuyan Yin,Yi-Xuan Wang,Cheng Hou,Jing Wang,Jing Xu,Xinbai Jiang,Dan Chen,Yang Mu,Jinyou Shen
Anammox coupled partial S 0 -driven autotrophic denitrification (PS 0 AD) technology represents an innovative approach for removing nitrogen from wastewater. The research highlighted the crucial role of biofilm on sulfur particles in the nitrogen removal process. Further analysis revealed that sulfur-oxidizing bacteria (SOB) are primarily distributed in the inner layer of the biofilm, while anammox bacteria (AnAOB) are relatively evenly distributed in inner and outer layers, with Thiobacillus and Candidatus Brocadia being the dominant species, respectively. Except for anammox and PS 0 AD processes, 15 N isotope labeling tests determined that sulfur reshaped nitrogen metabolism pathways, providing solid evidence for the occurrence of sulfammox process. SOB and AnAOB collaborate in nitrogen and sulfur conversion, with SOB-drived PS 0 AD processes reducing nitrate to nitrite for AnAOB to remove ammonia. Conversely, the nitrate produced from anammox process can be reused by SOB. Metagenomic analyses verified that SOB drove the PS 0 AD process through encoding soxBYZ gene, while AnAOB might play an important role in simultaneously driving the anammox and sulfammox processes. These findings underscore the importance of biofilm and clarify the nitrogen-sulfur cycle mechanisms within the coupled system.
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高影响因子
BIORESOURCE TECHNOLOGY
·
2026
·
IF 8.2
Shuyan Yin,Yuhui Fang,Shenglei Sun,Xiaocui Wang,Jin Li
Nitrate/nitrite makes a difference in MAB-based consortia induced by ZVI. • Highest TNRE of 94.03 % was achieved in treating high saline wastewater. • Sulfurimonas was enriched and cooperated with Ca. Scalindua to remove nitrogen. • Marine anammox and ZVI-AD collaboratively removed nitrogen from saline wastewater. Nitrogen removal mechanisms of marine anammox bacteria (MAB) induced by zero-valent iron (ZVI) were investigated to reveal the differences resulting from nitrate or nitrite under high-salinity (3.5 %) and low-temperature (15 ± 1 °C) stress. The one-time addition of ZVI (20 g) altered the microbial energy metabolism and electron transfer pathways. When nitrate served as the electron acceptor, ZVI-based autotrophic denitrification (ZVI-AD) served as the “engine”, providing essential nitrite for MAB through partial nitrate reduction, achieving a total nitrogen removal efficiency (TNRE) of 31.5 %. Conversely, when nitrite was the electron acceptor, marine anammox dominated nitrogen removal, while ZVI-AD acted as a recycler of the anammox by-product (nitrate), establishing a self-coupled nitrogen cycle with a superior TNRE of 94.0 %. Sulfurimonas and Candidatus Scalindua were the core functional microbes, exhibiting different preferences for nitrate and nitrite. Compared to the nitrite-added reactor, Sulfurimonas was enriched more in the nitrate-added reactor. Nevertheless, ZVI significantly promoted the enrichment of Candidatus Scalindua in the nitrite-added reactor, with its relative abundance increasing to 31.3 %. Furthermore, Feammox may have contributed to ammonium removal during the later stage. Overall, the selection of electron acceptors regulated the nitrogen removal mechanisms of ZVI-induced MAB consortia, providing new insights into intensifying nitrogen removal in high-salinity and low-temperature wastewater treatment. Download: Download high-res image (165KB) Download: Download full-size image
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高影响因子
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