JOURNAL OF THE BRAZILIAN CHEMICAL SOCIETY
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2015
·Vol. 26
·期 6
·1098-1110
·
IF 1.129
Yao Hui,Lu Yang,Han Juan,Wang Yun,Yan Yongsheng
As a pretreatment technique to trace analysis, polyoxyethylene lauryl ether (POELE10)- salt aqueous two-phase extraction system (ATPES) is coupled with high performance liquid chromatography to analyse thiamphenicol (TAP) in egg, milk and honey. The analysis of the phase behaviors of the ATPES found that the extraction efficiency and enrichment factor of TAP was influenced by the types of salts, the concentration of salt, the concentration of POELE10, the concentration of 1-propanol and the temperature. The optimized conditions of experiment were determined in the multi-factor experiment by using response surface methodology. The extraction efficiency of TAP is up to 99.59% and the optimal values of enrichment factor of TAP was 27 under the optimized conditions. The limit of detection and the limit of quantification of TAP are 0.5 µg kg−1 and 1.5 µg kg−1, which meet the needs of determination of trace or ultratrace TAP in food.
研究领域:
食品科学
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分析化学
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2015
·
IF 3.391
Yang Lu,Hui Yao,Chuang Li,Juan Han,Zhenjiang Tan,Yongsheng Yan
Polyoxyethylene lauryl ether (POELE10)-NaH 2 PO 4 aqueous two-phase extraction system (ATPES) is coupled with HPLC to analyze chloramphenicol (CAP) in aquatic product. Response surface methodology (RSM) was adopted in the multi-factor experiment to determine the optimized conditions. The extraction efficiency of CAP ( E %) is up to 99.42% under the optimal conditions, namely, the concentration of NaH 2 PO 4 , the concentration of POELE10, pH and temperature were 0.186 g·mL −1 , 0.033 g·mL −1 , 3.8 and 25 °C respectively. The optimal value of enrichment factor of CAP (F) was 22.56 when the concentration of NaH 2 PO 4 was 0.192 g·mL −1 , the concentration of POELE10 was 0.024 g/ml, pH was 4.2 and temperature was 30 °C. The limit of detection (LOD) and limit of quantification (LOQ) of this method are 0.8 μg·kg −1 and 1 μg·kg −1 , which meet the needs of determining trace or ultratrace CAP in food. The E % and F of this technique are much better than other extraction methods.
研究领域:
分析化学
·
食品安全
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DOI
高影响因子
Foods
·
2024
·Vol. 13
·期 2
·199
·
IF 4.7
Qisheng Yue,Jun Tian,Ling Dong,Linyan Zhou
As a by-product of pomegranate processing, the recycling and reuse of pomegranate pomaces (PPs) were crucial to environmentally sustainable development. Ultrasound-assisted aqueous two-phase extraction (UA-ATPE) was applied to extract the anthocyanins (ACNs) from PPs in this study, and the central composite design response surface methodology (CCD-RSM) and artificial neural network–genetic algorithm (ANN-GA) models were utilized to optimize the extraction parameters and achieve the best yield. The results indicated that the ANN-GA model built for the ACN yield had a greater degree of fit and accuracy than the RSM model. The ideal model process parameters were optimized to have a liquid–solid ratio of 49.0 mL/g, an ethanol concentration of 28 g/100 g, an ultrasonic time of 27 min, and an ultrasonic power of 330 W, with a maximum value of 86.98% for the anticipated ACN yield. The experimental maximum value was 87.82%, which was within the 95% confidence interval. A total of six ACNs from PPs were identified by utilizing UHPLC-ESI-HRMS/MS, with the maximum content of cyanidin-3-O-glucoside being 57.01 ± 1.36 mg/g DW. Therefore, this study has positive significance for exploring the potential value of more by-products and obtaining good ecological and economic benefits in the future.Keywords:by-product;cyanidin-3-O-glucoside;ultrasound-assisted aqueous two-phase extraction;genetic algorithm;pomegranate pomace
研究领域:
食品科学
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环境科学
·
化学工程
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生物技术
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2019
·
IF 5.399
Lei Wang,Wenxuan Li,Yuanyuan Liu,Wenjing Zhi,Juan Han,Yun Wang,Liang Ni
High cost and enzyme deactivation in purification process are the two main obstacles for the use of enzyme as green catalyst. The objective of this work was to overcome these limitations by developing a cost-effective aqueous two-phase system (ATPS) for efficient purification of enzymes with remarkable separation efficiency and high retention of enzyme activity. The ATPS was formed by thermo-responsive block copolymer PEG 113 -b-PNIPAM 149 and salt as phase-forming components combining economy, recovery and sustainability. This strategy fabricated block copolymer with specified molecular weight and low LCST, which not only achieved better phase splitting but also ensured easy recycling for block copolymer. The developed ATPS demonstrated excellent extraction and biocompatibility for bromelain in real sample with 94.87% separation efficiency and 77.06% activity, which were remarkably higher than those obtained in EOPOEO-based ATPS. The recycling of copolymer was introduced to minimize cost, with recovery efficiency of 90% in the five cycles.
研究领域:
生物化学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Hanliang Li,Lianzhu Lin,Mouming Zhao
This study aimed at recycling flavonoids with glycolipid lowering activity from Chenpi processing residue produced by essential oil extraction. The crude Chenpi flavonoids obtained by ethanol reflux mainly contained flavonone (hesperidin) and polymethoxyflavonoids (nobiletin, tangeretin, sinensetin, and isosinensetin), which complementally exerted strong glycolipid lowering activity as revealed by digestive enzyme inhibitory activity, molecular docking and network pharmacology. The reducing sugar and water-soluble impurities were efficiently removed by yeast fermentation and aqueous two-phase system (ATPS) extraction, respectively. The optimal combination use of yeast (2 % w / v , CN36) and ATPS formed by 22 % ( w /w) (NH 4 ) 2 SO 4 and 25 % (w/w) ethanol improved flavonoid purity to 60.25 %, enriched flavonone and polymethoxyflavonoids by 6.69-fold, and enhanced digestive enzyme inhibitory activity, which was superior to the use of individual yeast or ATPS, unoptimized combination, macroporous resin (XAD-16), and ethyl acetate. This study was the first to develop a recyclable biophysical method for simultaneous enrichment of flavonone and polymethoxyflavonoids.
研究领域:
药学
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生物技术
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食品科学
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药理学
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产品关联
DOI
PubMed
高影响因子
Journal of the Iranian Chemical Society
·
2016
·Vol. 13
·期 10
·1759-1765
·
IF 1.3
Wang Tingting,Zhang Dan,Zhang Ziliang,Han Juan,Wang Yun,Tang Xu
As a novel and green pretreatment technique to trace samples, polyoxyethylene cetyl ether (POELE20)–(NH 4 ) 2 SO 4 aqueous two-phase extraction system was coupled with high-performance liquid chromatography to analyse synchronously chloramphenicol (CAP), thiamphenicol (TAP) and florfenicol (FF) in chicken and pork samples. It was found that the extraction efficiency ( E %) and enrichment factor ( F ) of the three antibiotics were influenced by the types of salts, the concentration of salt, the concentration of POELE20, system temperature and pH. The final optimal condition was as following: the phase-forming salt is (NH 4 ) 2 SO 4 , the concentration of (NH 4 ) 2 SO 4 is 0.141 g mL −1 , the concentration of POELE20 is 0.03 g mL −1 , the temperature is 298.15 K, and the system pH is 4.5. This POELE20–(NH 4 ) 2 SO 4 ATPS was applied to separate and enrich three antibiotics in real sample under the optimal conditions, and it was found that the recovery was 97.20–102.00 % with a RSD of 0.61–4.85 %. The limit of detection for CAP, TAP and FF were 0.10, 0.50 and 0.50 μg kg −1 , and the limit of quantitation for CAP, TAP and FF was 0.15, 1.50 and 1.50 μg kg −1 . Seven times the experiments were used to verify the repeatability and veracity of this method, and the RSD for the intra-day and inter-day were 1.13–3.22 and 1.74–4.72 %.
研究领域:
分析化学
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食品科学
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药物分析
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DOI
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
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2022
·
IF 6.633
Yanrong Cheng,Xia Guo,Delan Ge,Wenlin Xu,Yaqiong Wang
Broadening the polarity range of polymer-based aqueous two-phase systems (ATPSs) is of practical significance. Recently, ionic liquids have been used as adjuvants to modify the polarity and properties of PEG-rich phases in PEG/salt ATPSs. However, thus far, only short alkyl (containing less than 8 carbon atoms)-chained ionic liquids have been able to promote the extraction performance of ATPSs; long alkyl-chained ionic liquids have decreased the extraction performance significantly due to their distribution in the bottom phase. In the present study, we found that by complexing with Triton X-100 (TX-100), long alkyl-chained imidazolium ionic liquids [C n mim]Br (n = 10 and 12) as adjuvants were enriched in the top phase of PEG/salt ATPSs, and hence, the polarity difference of the two phases were increased. By extracting two model substances (S-mandelic acid (MA) and l -tryptophan (Trp)), it was concluded that, although the long alkyl-chained [C n mim]Br adjuvant decreased the extraction performance of the ATPS substantially, the long alkyl-chained [C n mim]Br/TX-100 complex as the adjuvant enhanced the extraction performance of the ATPS significantly. Compared with the case without an adjuvant, when the [C 12 mim]Br/TX-100 complex and the [C 10 mim]Br/TX-100 complex were used as the adjuvants, the partition coefficients of MA and Trp in the PEG 600/(NH 4 ) 2 SO 4 ATPS were increased up to 3 ∼ 4 times, much better than those with short alkyl-chained ionic liquid as the adjuvant. Moreover, the [C 12 mim]Br/TX-100 complex is more conductive to the transfer of MA into the PEG-rich phase, and the [C 10 mim]Br/TX-100 complex is more conductive to the transfer of Trp into the PEG-rich phase.
研究领域:
生物化学
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分离科学
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工业化学
✓ 已验证
产品关联
DOI
高影响因子
Analytical Methods
·
2016
·Vol. 8
·期 35
·6566-6572
·
IF 1.915
Xiaozhong Wang,Xuwen Li,Lanjie Li,Min Li,Qian Wu,Ying Liu,Jie Yang,Yongri Jin
A green and effective extraction system based on the use of an ultrasound-assisted ionic liquid aqueous two-phase system (IL-ATPS) was developed for the determination of aconitum alkaloids in lateral roots of Aconitum carmichaeli Debx (Fuzi in Chinese). IL-ATPS parameters, including the type and amount of ionic liquid, amount of salts, and pH value, as well as extraction time, were investigated. Under the optimized conditions, the recoveries of the analytes varied from 80.37 to 107.18%. The detection limits and quantification limits for the analytes were achieved within the ranges of 0.048–0.082 μg g−1 and 0.16–0.30 μg g−1, respectively. After extraction, the recovery of ionic liquids without the use of organic solvents was also confirmed. The hydrophilic ionic liquids (1-hexyl-3-methylimidazolium bromide) were converted into hydrophobic ionic liquids (1-hexyl-3-methylimidazolium hexafluorophosphate) by a metathesis reaction, and in situ liquid–liquid microextraction was triggered simultaneously. Finally, 0.1 mol L−1 HCl aqueous solution (containing 5% Tween-20) was used as the back-extraction agent to recover the ionic liquids.
研究领域:
中药学
·
分析化学
✓ 已验证
DOI
高影响因子
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2018
·
IF 3.141
Pan Sun,Kun Huang,Weiyuan Song,Zhen Gao,Huizhou Liu
An ionic-liquid-based aqueous two-phase system (iL-ATPS) consisting of an ionic liquid, tributylmethylammonium nitrate (N4441NO3), and NaNO3 aqueous solutions was developed for the extraction and separation of rare-earth ions, for example Nd(III), from transition-metal ions. The excellent selectivity of a green nonfluorinated ionic liquid and the low-viscosity feature of an aqueous two-phase system were combined in our suggested iL-ATPS. The results indicated that efficient separation of Nd(III) from transition-metal ions, Fe(III), Ni(II), and Co(II), could be obtained in our suggested iL-ATPS. Furthermore, different parameters of the separation process, including the concentrations of HNO3 and NaNO3 and the added amount of N4441NO3, were optimized. Notably, the extraction kinetics of Nd(III) could be improved significantly in the present iL-ATPS compared with the conventional organic-solvent-based ionic-liquid extraction systems. The similar environment across the liquid–liquid interface was revealed by the ultralow viscosity and interfacial tension of the ionic-liquid-rich phase in the iL-ATPS. This feature was responsible for the enhanced extraction kinetics of Nd(III). In addition, the structures of the extraction complexes were elucidated by using NMR spectroscopy and molecular simulations. It was demonstrated that NO3– ions were in the inner coordination shell of Nd(III) ions to interact with Nd(III) ions, while N4441+ surrounded Nd(III) ions at the outer coordination shell of Nd(III) ions. Moreover, 2 mol/L HNO3 aqueous solutions could be used to effectively strip Nd(III) from the loaded ionic-liquid-rich phase. After 5 cycles, the extraction performance of the ionic liquid remained nearly unchanged. The present work highlights an environmental method to selectively separate rare-earth ions from the complicated solutions coexisting with other transition-metal impurity ions. It provides a basis for the future application in recycling of rare-earth elements from the spent NdFeB magnets.
研究领域:
环境化学
·
湿法冶金
·
可持续回收
·
分离科学
·
稀土元素
✓ 已验证
DOI
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2016
·
IF 3.13
Yang Lu,Biao Cong,Zhenjiang Tan,Yongsheng Yan
Polyoxyethylene lauryl ether (POELE10)-Na 2 C 4 H 4 O 6 aqueous two-phase extraction system (ATPES) is a novel and green pretreatment technique to trace samples. ATPES coupled with high-performance liquid chromatography (HPLC) is used to analyze synchronously sulfadiazine (SDZ) and sulfamethazine (SMT) in animal by-products (i.e., egg and milk) and environmental water sample. It was found that the extraction efficiency (E%) and the enrichment factor (F) of SDZ and SMT were influenced by the types of salts, the concentration of salt, the concentration of POELE10 and the temperature. The orthogonal experimental design (OED) was adopted in the multi-factor experiment to determine the optimized conditions. The final optimal condition was as following: the concentration of POELE10 is 0.027 g mL −1 , the concentration of Na 2 C 4 H 4 O 6 is 0.180 g mL −1 and the temperature is 35 °C. This POELE10-Na 2 C 4 H 4 O 6 ATPS was applied to separate and enrich SDZ and SMT in real samples (i.e., water, egg and milk) under the optimal conditions, and it was found that the recovery of SDZ and SMT was 96.20–99.52% with RSD of 0.35–3.41%. The limit of detection (LOD) of this method for the SDZ and SMT in spiked samples was 2.52–3.64 pg mL −1 , and the limit of quantitation (LOQ) of this method for the SDZ and SMT in spiked samples was 8.41–12.15 pg mL −1 .
研究领域:
分析化学
·
环境科学
·
食品科学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）