Analytical Methods
·
2016
·Vol. 9
·期 2
·282-286
·
IF 1.915
B. Liu,X. P. Wang,X. Y. Sun,C. X. Yang,P. Li
In this paper, a novel fluorescent immunosensor based on self-assembled multilayers was constructed to detect microcystin-(leucine–arginine). CdTe quantum dots were firstly assembled on the quartz substrates. Then bioconjugates were formed by an amidation reaction between CdTe quantum dots and microcystin-(leucine–arginine) antibodies. The sensing system exhibited a linear range from 0.0001 to 3 μg L−1 for microcystin-(leucine–arginine) with a detection limit of 0.0440 ng L−1, 2–4 orders of magnitude lower than most other fluoroimmunoassays. Desirable results were also obtained in natural lake water and interference experiments. The fluorescent immunosensor based on self-assembled multilayers has great potential for the rapid, sensitive detection of trace amounts of microcystin-(leucine–arginine) in real water samples.
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ACS Applied Nano Materials
·
2025
·
IF 5.5
Qi Tao,Hang Yao,Zehao Gu,Xiaofei Yang,Zhonglian Wu,Dong-An Wang,Yu Cao,Hui Chong
Chronic wounds are susceptible to bacterial aggregation, which could lead to severe infections and a complicated healing process. Addressing bacterial infections is crucial for effective wound repair. In this study, we developed an antibacterial nanozyme sustained-release system, Arg-CDs/ZIF-8, by doping arginine carbon dots (Arg-CDs) into the zeolitic imidazolate framework (ZIF-8) to achieve pH-responsive release. ensuring prolonged action at the wound site. In the presence of hydrogen peroxide, Arg-CDs/ZIF-8 demonstrates superior generation of NO and reactive oxygen species capability, exhibiting promising synergistic antibacterial effects against E. coli and S. aureus. In vivo animal experiments also demonstrated the excellent wound-healing ability of Arg-CDs/ZIF-8 in the presence of H2O2. The in vivo toxicity results confirm that Arg-CDs/ZIF-8 has good biosafety, providing a promising nonantibiotic approach to combat wound infections, and highlighting its potential in the development of antimicrobial materials.
研究领域:
生物材料学
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医学
·
纳米医学
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高影响因子
RSC Advances
·
2015
·Vol. 5
·期 20
·15668-15673
·
IF 3.84
Xian-Dong Lang,Shuai Zhang,Qing-Wen Song,Liang-Nian He
An easily prepared amino acid ionic liquid (AAIL) i.e. [TBP][Arg] comprising a tetra-butylphosphonium cation and an arginine anion was found to be an efficient and recyclable catalyst for the synthesis of quinazoline-2,4(1H,3H)-diones from 2-aminobenzonitriles and CO2 under solvent-free conditions. As a result, various 2-aminobenzonitriles bearing electron-withdrawing or electron-donating substituents worked well to afford quinazoline-2,4(1H,3H)-diones in excellent yields. Notably, this type of AAIL showed good stability, and could be easily recovered and reused five times without significant loss of its catalytic activity. This process represents an alternative approach for greener chemical fixation of CO2 to afford valuable compounds.
研究领域:
有机化学
·
环境科学
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高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2021
·
IF 4.098
Tianran Wang,Qidan Pang,Zhipu Tong,Hanyue Xiang,Nao Xiao
A simple probe BHN based on naphthol and benzothiazole is reported for detecting of arginine (Arg) and lysine (Lys) with high selectivity and sensitivity. The BHN in aqueous solution upon reacting with Arg or Lys induced a visible color change from colorless to yellow. The probe BHN can also be employed for fluorescence turn-on sensing of Arg and Lys with the limits of detection (LOD) of 5.20 × 10 -2 μM and 3.69 × 10 -2 μM, respectively. The naked eye colorimetric and fluorimetric detecting is lack of sensitive to other common amino acids including Gly, Ala, Ser, Pro, Val, Thr, Cys, Leu, Ile, Asn, Asp, Glu, Gln, Met, His, and Phe. The sensing mechanism has been proposed by pH investigation and 1 H NMR spectra.
研究领域:
分析化学
·
生物化学
·
光谱学
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PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2016
·
IF 3.902
Song Luo,Yixin Zhang,Jun Cao,Bin He,Sai Li
A new drug delivery system with improved endocytosis efficiency based on arginine modified poly(ethylene glycol)- b -poly(ε-caprolactone) (PEG-PCL) diblock copolymers was developed successfully. The structures of amphiphilic copolymers were verified by proton Nuclear Magnetic Resonance ( 1 H NMR), Fourier Transform Infrared (FTIR) and Gel Permeation Chromatography (GPC). The amphiphilic copolymers could self-assemble into spherical micelles with critical micelle concentration (CMC) at approximately 0.03 mg/mL. Biocompatibility, cellular internalization efficiency and in vitro antitumor effect of the polymeric micelles were studied. Results showed that drug-free micelles were nontoxic to cells. The Confocal Laser Microscopy (CLSM) and the flow cytometry as well as the antitumor activity tests all revealed that drug-loaded micelles modified by arginine groups (guanidino) exhibited higher endocytosis efficiency than those without modification, resulting in a lower IC 50 value to kill tumor cells and meanwhile averting the “competitive binding” problem. Also, in vivo results demonstrated that arginine modified polymeric micelles could deliver the drug to tumor site more efficiently than ones without any modification, thus indicating guanidino in polymeric micelles could benefit the internalization of micelles to fully exert the function of killing tumors. In general, guanidinylation strategy in this splendid novel micelle system has a great potential to improve the therapeutic effect of nanoparticle-based drug delivery system for cancer therapy in the future.
研究领域:
药学
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生物医学工程
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肿瘤学
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PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2020
·Vol. 44
·期 12
·4842-4849
·
IF 3.288
Ziting Zhang,Zhefeng Fan
As a new-type of nanomaterial, carbon dots (CDs) have been extensively applied in biochemistry-related fields. In this study, a single-step hydrothermal method was used to rapidly and conveniently synthesize Mg-doped CDs (Mg-CDs) with stable fluorescence, and favorable water solubility and dispersity, with the quantum yield reaching 26.86%. With adenosine triphosphate (ATP) as a switch, a fluorescent sensor was developed to monitor arginine based on the Mg-CDs. The surface of the Mg-CDs had many organic functional groups, and ATP could bind effectively to these groups, thereby quenching the fluorescence of the Mg-CDs. In addition, the electrostatic interaction between arginine and ATP displayed covalent stability, which enhanced the fluorescence of the Mg-CDs. After constructing the probe sensor, we also systematically optimized the experimental conditions and investigated parameters such as pH and ATP concentration. Under the optimum test conditions, the constructed fluorescence test sensor revealed a linear relationship with arginine within the range of 0.3–130 μmol L−1, with a low detection limit of 0.15 μmol L−1. The sensor exhibited high specificity, excellent stability and good reproducibility. The results showed the satisfactory determination of arginine in human urine with a recovery of 97.0–100%. The relative standard deviation value was less than 4.1%. The present study can be used as a highly selective and sensitive colorimetric method for the quantification of arginine from various aqueous media.
研究领域:
生物化学
·
纳米材料科学
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高影响因子
MOLECULAR PHARMACEUTICS
·
2024
·
IF 4.5
Shuai Hong,Changxiu Lin,Junsheng Hu,Jingshu Piao,Ming Guan Piao
Nimodipine is the primary clinical drug used to treat cerebral vasospasm following subarachnoid hemorrhage. Currently, tablets have low bioavailability when taken orally, and injections contain ethanol. Therefore, we investigated a new method of nimodipine administration, namely, nasoencephalic administration. Nasal administration of nimodipine was carried out by attaching the cell-penetrating peptide octa-arginine (R8) to liposomes of nimodipine and incorporating it into a temperature-sensitive in situ gel. The prepared liposomes and gels underwent separate evaluations for in vitro characterization. In vitro release exhibited a significant slow-release effect. In vitro toad maxillary cilia model, RPMI 2650 cytotoxicity, and in vivo SD rat pathological histotoxicity experiments showed that all the dosage from the groups had no significant toxicity to toad maxillary cilia, RPMI 2650 cells, and SD rat tissues and organs, and the cilia continued to oscillate up to 694 ± 10.15 min, with the survival rate of the cells being above 85%. A transwell nasal mucosa cell model and an isolated porcine nasal mucosa model were established, and the results showed that the osmolality of the R8-modified nimodipine liposomal gel to nasal mucosal cells and isolated porcine nasal mucosa was 30.41 ± 2.14 and 65.9 ± 7.34 μg/mL, respectively, which was significantly higher than that of the NM-Solution and PEGylated nimodipine liposome gel groups. Animal fluorescence imaging studies revealed that the R8-modified nimodipine liposomal gel displayed increased brain fluorescence intensity compared to the normal liposomal gel. Pharmacokinetic results showed that after transnasal administration, the AUC(0–∞) of the R8-modified nimodipine liposomal gel was 11.662 ± 1.97 μg·mL–1, which was significantly higher than that of the plain nimodipine liposomal gel (5.499 ± 2.89 μg·mL–1). Brain-targeting experiments showed that the brain-targeting efficiencies of the PEGylated nimodipine liposome gel and R8-modified PEGylated nimodipine liposome gels were 20.44 and 33.45, respectively, suggesting that R8/PEG/Lip-NM-TSG significantly increased the brain-targeting of the drug.
研究领域:
药学
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神经学
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药物递送系统
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药理学
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生物技术
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PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2026
·
IF 13.8
Qingqing Li,Sitong Zhang,Zhiyi Wu,Guangjun Huang,Jianlong Wang
Photodynamic inactivation holds promise for food packaging, yet its application is hindered by poor efficiency against Gram-negative bacteria. This study developed an active composite film based on food-grade, positively-charged chitosan (CS) and a hierarchically structured composite (HPZA). Under visible light illumination, HPZA generated substantial reactive oxygen species, causing bacterial membrane disruption and subsequent ion leakage. As a result, HPZA at 60 μg/mL achieved a 5 log 10 reduction in Escherichia coli , and at 80 μg/mL it reduced Staphylococcus aureus from 6 log 10 to 1 log 10 CFU/mL. Furthermore, it exhibited potent light-driven antifungal activity against Botrytis cinerea . With HPZA as a functional active filler, the CS-based films exhibited enhanced physicochemical performance, including a 53.48% increase in elongation at break, a 24.27% increase in water contact angle, and increased visible light absorbance. In a practical application, the CS-HPZA 2.00% film effectively extended the shelf life of cherry tomatoes from 2 to 10 days, while maintaining fruit firmness and total soluble sugar content, with minimal difference from the fresh group. These findings highlight CS-HPZA as a promising light-driven antimicrobial packaging film for active food packaging applications.
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高影响因子
FOOD RESEARCH INTERNATIONAL
·
2026
·
IF 8.8
Yushi Li,Jinmei Li,Jiteng Deng,Pei Xia,Guicheng Zhou,Zhenjun Zhu,Yu Ding,Jingge Yang,Fen Zhang
Phocaeicola vulgatus -centered network and ornithine synthesis are impaired in obese individuals. • P. vulgatus alleviates HFD-induced obesity in humanized rats. • P. vulgatus shows a potential functional association with Phascolarctobacterium faecium in enhancing arginine biosynthesis. • Elevated systemic arginine is associated with activated host hepatic retinoic acid signaling. • The arginine-retinoic acid axis highlights P. vulgatus as a probiotic target. Fecal microbiota transplantation (FMT) shows inconsistent clinical efficacy in treating obesity, and the specific microbial determinants dictating its success remain poorly characterized. Our previous clinical FMT trial identified Phocaeicola vulgatus as a key microbe contributing to the therapeutic efficacy of obesity treatment. Here, to investigate its role in obesity, we established an independent clinical cohort comprising obese and lean individuals, revealing that the P. vulgatus -centered network and ornithine synthesis are impaired in the obese group. We then confirmed causality by utilizing a humanized rat model carrying microbiota from a P. vulgatus -deficient obese patient, demonstrating that P. vulgatus supplementation significantly mitigates HFD-induced obesity, including reductions in body weight and serum total cholesterol levels, as well as the alleviation of hepatic steatosis. To further explore the functional mechanisms of P. vulgatus , integrated metagenomic and metabolomic analyses revealed a potential functional association between P. vulgatus and Phascolarctobacterium faecium that is associated with enhanced intestinal arginine biosynthesis and systemic availability. Furthermore, hepatic transcriptomics linked these elevated circulating arginine levels to the upregulation of retinoic acid (RA) signaling. Taken together, our findings outline a potential microbial-host network wherein P. vulgatus mitigates obesity via the arginine-RA axis, providing a valuable scientific basis for exploring this strain as a probiotic candidate for metabolic health. Download: Download high-res image (165KB) Download: Download full-size image
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RSC Advances
·
2025
·Vol. 15
·期 16
·12528-12537
·
IF 4.6
Junbo Wang,Jie Jiang,Jing Wang,Shouyuan Hu,Jiahao Hu,Yalong Li,Zhiyu Tao,Chengju Wu,Pei Li,Liang Chen
Nanofiltration has emerged as an effective technique for the selective separation of mono- and divalent ions, such as Mg2+/Li+ mixtures, and plays a crucial role in lithium extraction from salt lakes. In this study, a graphene oxide (GO) membrane with positively charged channels was prepared by crosslinking arginine (Arg) onto GO nanosheets, followed by vacuum filtration to form the membrane, and then thermal reduction (Arg-rGO). The Arg-rGO membrane exhibits high performance in the ion separation of a typical brine with a Mg2+/Li+ mass ratio of 20. The separation factor (SLi/Mg) reached up to 45.6—two times the highest separation factor reported—while maintaining an advanced water permeance of 21.3 L m−2 h−1 bar−1. Furthermore, the Mg2+/Li+ mass ratio was reduced from 20 to 0.2 after two-stage crossflow filtration with high flux under high pressure. The observed separation performance can be attributed to the synergistic effect of electrostatic repulsion and size-exclusion. These findings confirmed efficient separation of Mg2+/Li+ using GO membranes, demonstrating potential for practical application in lithium extraction from salt lakes.
研究领域:
材料科学
·
环境工程
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PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2025
·
IF 4.6
Yidan Liang,Mingyu Cui,Yujie Zhao,Bo Han,Ran Li,Yuqi Zhang,Ji-Jiang Wang
A novel colorimetric-fluorometric probe, 3-hydroxy-[1,1′:4′,1″-terphenyl]-4-carbaldehyde (HTPC), was synthesized for the detection of basic amino acids lysine (Lys) and arginine (Arg). The response of HTPC was driven by the acid-base reaction between HTPC and Lys/Arg. Upon addition of Lys/Arg, the HTPC acetone-H 2 O solution generated new electronic absorption at ~400 nm and turned-on a fluorescence emission at ~500 nm, and both the absorbance and fluorescence intensities significantly enhanced. Simultaneously, the solution underwent a color change from colorless to light-yellow and emitted blue-green fluorescence under UV light irradiation. This is because Lys/Arg caused deprotonation of the phenolic hydroxyl groups in HTPC using their terminal amino groups, thereby altering the electron cloud distribution of HTPC and enabling independent existence of the fluorophore, which enhanced both absorbance and fluorescence intensity. Furthermore, Lys and Arg could be distinguished by treating the probe-amino acid solutions with Hg 2+ and monitoring the fluorescence property. The limit of detection of Lys and Arg were 8.50 μM and 2.77 μM for colorimetric detection, and 1.92 μM and 2.35 μM for the fluorescence detection, respectively. The synthesized probe was employed to accurately quantify the Lys/Arg contents in drugs and achieved live-cell fluorescence imaging of Lys/Arg. Our synthesized colorimetric-fluorometric probe based on the acid-base reaction provides a new strategy for constructing sensitive probes of amino acids.
研究领域:
分析化学
·
生物有机化学
·
传感器与探针
·
氨基酸检测
·
荧光成像
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PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2016
·
IF 2.76
Zhan-Chao Wu,Zhao-Zhan Wang,Jie Liu,Jin-Hua Yin,Shao-Ping Kuang
A new adsorbent l -Arg-CS/Fe 3 O 4 was prepared by l -arginine modifying magnetic chitosan for the removal of Cu(II ) ions from aqueous solution. The compositions of l -Arg-CS/Fe 3 O 4 were characterized by infrared (IR) spectroscopy, X-ray diffraction (XRD) analysis and thermogravimetric (TG) analysis, respectively. The surface morphology, particle size and specific surface area were measured on scanning electron microscope (SEM) and micromeritics gas adsorption surface analyzer. The adsorption behavior of l -Arg-CS/Fe 3 O 4 for Cu(II ) were investigated as a function of pH, contact time and initial Cu(II ) concentration. The maximum adsorption capacity for Cu(II ) was 2.13 mmol g −1 at the optimized adsorption conditions, which were pH = 5.5, t = 13 min, C 0 = 4.0 mmol L −1 . The adsorption behavior was evaluated by the analysis of adsorption kinetics. Freundlich and Langmuir isotherm models were used to estimate the adsorption mode. l -Arg-CS/Fe 3 O 4 exhibited high adsorption capability after five regeneration cycles. The adsorption and desorption mechanisms were discussed. The results show that l -Arg-CS/Fe 3 O 4 is a promising adsorbent for removal of Cu(II) from aqueous solutions.
研究领域:
环境科学
·
材料科学
·
化学工程
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高影响因子
危险品化学品经营许可证（带存储）