MACROMOLECULAR CHEMISTRY AND PHYSICS
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2016
·Vol. 217
·期 10
·1197-1204
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IF 2.495
Jia Zhang,Wei Ji,Tian Liu,Chuanliang Feng
Syneresis property limits the further application of Kappa-carrageenan (κ-CG) in cell culture scaffold. To address this problem, κ-CG hydrogel is tailored by blending with a C2-symmetric benzene-based supramolecular gelator (B2). Scanning electron microscope (SEM) and Fourier transform infrared (FT-IR) spectroscopy confirm the semiinterpenetrating (semi-IPN) structure of the blended hydrogels and their wettability or thermal stability is also found to be improved. Based on the reduced syneresis, the growth and proliferation of the cultured HCT 116 cells on the blended hydrogels is enhanced compared with pure κ-CG hydrogels. This study paves an easy and feasible way to achieve stable and biocompatible κ-CG/B2 blended hydrogels with high water retention and it helps broaden the promising applications of κ-CG hydrogels in tissue engineering.
研究领域:
材料科学
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生物医学工程
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组织工程
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DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2019
·
IF 4.784
Liu Kunliang,Jin Zhicheng,Hu Xiaolong,Yan Dan,Zhang Yu,Zhu Haidong,Teng Gaojun,Xiong Fei
Trans-arterial chemoembolization (TACE) has been a mainstream treatment for hepatocellular carcinoma (HCC), accordingly there are more requirements for embolic agents’ properties, mainly concentrated on biodegradability and imaging function. A new kind of biodegradable multifunctional porous microspheres (BMPMs) with internal coarse plications are ingeniously designed and manufactured, consisting of carrageenan, inhexol and superparamagnetic iron oxide nanoparticle (SPIO). BMPMs’ sound roundness and excellent swelling behavior guarantee them to be smoothly transported into appointed arteries’ ends and achieve satisfactory embolization effect. Porous structure together with sulphate groups on BMPMs’ skeleton weaved by cross-linked carrageenan contributes to a marvelous doxorubicin hydrochloride (Dox) loading capacity over 45 mg Dox per mL of swollen microspheres, as well as a controllable degradation rate by allowing enzymes to infiltrate into BMPMs through holes, degrading 20–35% after two months in vitro . Inner coarse plications enable BMPMs to effectively combine with iohexol via hydrophilic effect and SPIO by blocking, which can further exhibit a decent imaging performance under digital subtraction system (DSA) and magnetic resonance imaging (MRI) for optimization of current TACE and development of a more advanced imageable TACE (iTACE). Safety evaluation and animal experiment manifest that BMPMs are reliable enough and hold great potential and competitiveness of being used in curing HCC.
研究领域:
生物医学工程
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肿瘤学
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材料科学
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医学影像
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Shuo Shi,Xi Lan,Xiyan Ding,Ximei Han,Jing Sun,Jianlong Wang,Jinyou Duan
The burdens of microbial food safety and environmental contamination make it necessary to search sustainable, safe, antibacterial and antioxidant active food packaging materials. This contribution proposed the use of copper-ferulic acid networks (CuFA NWs) as antibacterial substances. By immobilizing CuFA NWs into carrageenan matrix, a CuFA network-reinforced carrageenan-based packaging film (Carr/CuFA) was obtained via spontaneously hydrogen bond and electrostatic interaction indicated by ATR-IR and XPS. Interestingly, the addition of CuFA NWs increased the mechanical strength, surface hydrophobicity, and water vapor barrier properties of the carrageenan-based film, and imparted the film with UV-shielding capacity. Importantly, the Carr/CuFA x film exhibited effective antioxidant activity, and antibacterial performance against four foodborne bacteria. As a result, after confirming the safety of Carr/CuFA 3 films by releasing, hemolysis and cell viability experiments, the Carr/CuFA 3 film exhibited great potential in the safety control and preservation of fresh fruit by using blueberry and cherry as model fruit. In summary, this work provides a feasible candidate for the preservation and contamination control of fresh fruit.
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高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Meigui Huang,Lixia Cong,Ruifeng Ying,Mehraj Ahmad,Gang Hao,Khizar Hayat,Ahmad Mohammad Salamatullah
The intense bitterness of quercetin poses a challenge to its utilization in the food industry. To address this issue, three anionic polysaccharides (carrageenan, pectin, and trehalose) were individually incorporated to fabricate polysaccharide-coated liposome nanocarriers. Electronic tongue analysis revealed a significant decreasing bitterness value (10.34 ± 0.07 mV, sensory score 1.8 ± 0.2, taste weak bitter) in quercetin-loaded nanoliposomes , compared with the bitterness value of quercetin aqueous solution (14 ± 0.01 mV, sensory score 7.3 ± 0.3, taste strong bitter). Furthermore, the polysaccharide-coated nanoliposomes exhibited an even greater capacity to mask the bitterness of quercetin, with carrageenan coated nanoliposomes demonstrating the most pronounced effect. The superior bitter masking ability of carrageenan coated nanoliposomes can be attributed to its high charge and viscosity. In sensory evaluations, gummy incorporated with carrageenan-coated nanoliposomes received the highest ratings, exhibiting enhanced overall palatability and antioxidant activity . This study offers insights into expanding the use of bitter nutrients in food applications and paves the way for more appealing and healthful food products.
研究领域:
食品科学
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纳米技术
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药剂学
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产品关联
DOI
PubMed
高影响因子
Food Packaging and Shelf Life
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2024
·
IF 8.5
Yahong Han,Mingrui Zhou,Jiajie Deng,Cheng Cheng,Zihan Xu,Wenfu Hou,Yang Yi,David Julian McClements,Shuai Chen
Recently, intelligent packaging integrated with natural colorants has garnered interest for its capability to assess the freshness of stored food and delay its spoilage. However, the poor dispersibility and stability of natural pigments limit their application. This study addresses these issues by encapsulating natural pigments in core-shell protein-polysaccharide nanoparticles to enhance their stability and dispersibility. Using curcumin and anthocyanin as examples, pigment-loaded nanoparticles were synthesized using antisolvent precipitation to create zein particles, which were then coated with chondroitin sulfate through electrostatic layer-by-layer deposition. This method produced spherical anionic nanoparticles with high encapsulation efficiency (Curcumin: 91.93 %, Anthocyanin: 85.85 %). These nanoparticles were incorporated into carrageenan-based composite films, which acted as freshness indicators and preservatives. The films' color shifted from green-brown ( L =33.47, a =9.46, b =11.09) to red-brown ( L =22.80, a =13.83, b =2.11) with increasing nanoparticle concentration (from 20 % to 40 % w/v), and their uniform microstructure demonstrated good compatibility with the biopolymer matrix. The films were tested as freshness indicators and preservatives for packaged fish, showing improved freshness detection and preservation effectiveness with higher nanoparticle content. These biodegradable materials are expected to be intelligent packaging solutions for the food sector.
研究领域:
食品科学
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材料科学
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DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2026
·
IF 8.7
Gerui Ren,Ruiqi Hu,Jiajun Mao,Xinpei Cai,Tianrong Wang,Xin Song,Wentao Wu,Caixin Zhuge,Min Huang,Hujun Xie,Kejun Cheng
Dihydromyricetin (DMY) possesses potent antioxidant, anti-inflammatory and neuroprotective activities, yet its potential applications are curtailed by extremely low aqueous solubility and limited bioaccessibility. To overcome these constraints, the Zein-DMY/κ-carrageenan (κ-CGN) nanoparticles were engineered via anti-solvent precipitation at pH 5 (zein:κ-CGN mass ratio of 6:4) that reached 83.1% encapsulation efficiency. Multispectral analysis (FTIR, fluorescence, XRD) combined with molecular docking showed that hydrogen bonding, electrostatic and hydrophobic interactions were the main driving forces for the formation of nanoparticles, and DMY was anchored in the hydrophobic region of Zein. The dense sulfate groups of κ-CGN electrostatically stabilized the surface of nanoparticles, preventing their aggregation. Compared with free DMY, the nanocomposite retained strong antioxidant activity (95.25% for DPPH and 97.20% for ABTS), increased water solubility 4.88-fold (3.12 ± 0.07 mg/mL) and enhanced bioaccessibility 1.65-fold (37.31%). In vitro digestion simulations, coupled with SEM and release kinetics modeling, demonstrated the controlled release characteristics of nanoparticles in intestinal fluid. The release kinetics closely adhered to the Ritger-Peppas model, indicating Fickian diffusion behavior. This scalable, food-grade nanocarrier offers a robust strategy to enhance the solubility, bioaccessibility and targeted intestinal delivery of DMY for functional foods and nutraceuticals.
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PubMed
高影响因子
FOOD CHEMISTRY
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2014
·
IF 3.259
Jinbing Li,Xiaoyong Wang
Novel thermally-induced BSA/ι-carrageenan particles are used as a protective carrier for (−)-epigallocatechin-3-gallate (EGCG). The addition of EGCG to BSA/ι-carrageenan particles can highly quench the intrinsic fluorescence of BSA , which is explained in terms of the binding of EGCG to the hydrophobic pockets of BSA mainly through the hydrophobic force. According to the double logarithm equation, the binding constant is determined as 1.1 × 10 8 M −1 for the binding of EGCG with BSA/ι-carrageenan particles. The high binding affinity is ascribed to both the molecular structure of EGCG and the partial unfolding state of BSA in BSA/ι-carrageenan particles. The circular dichroism spectra and calculated α-helix of BSA suggest that the bound EGCG leads to a more random secondary structure of BSA. Furthermore, BSA/ι-carrageenan particles are found to be superior to native BSA and pure BSA particles for improving the stability and radical scavenging activity of EGCG.
研究领域:
食品科学
·
生物化学
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DOI
高影响因子
SYNTHETIC METALS
·
2013
·
IF 2.109
Dan Zhu,Qiaoli Guo,Zhaoyu Zheng,Masaru Matsuo
There are some biosensors and biochips for the recognition and quantization for particular biological molecules. They are made of conductive polymers and natural biomolecules such as protein, DNA and polysaccharides. The conductive polymers work as electric wires for signal transmission, while the natural biomolecules are receptors. Such electric devices are of complex and heterogeneous structures, in either single chain or condensed chains or dispersive phase separations. In order to investigate the complex structures of the polymer blends in the devices and the relation between the structure and the electrical properties, we have prepared several kinds of nano-structured polyaniline (PANI) with defined aggregate morphologies, i.e., nano-granules or nano-fibers with different aspect ratios. The blends of κ-carrageenan and PANIs have been prepared through sol–gel process. The multi-level structures of PANIs have been characterized with X-ray diffraction (XRD) and scanning electron microscope (SEM). The thermal analysis and dielectric relaxation spectroscopy help to evaluate and to understand the chain relaxation and the interfacial structures of the blends.
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DOI
高影响因子
FOOD HYDROCOLLOIDS
·
2020
·
IF 7.053
Luping Gu,David Julian McClements,Jiaying Li,Yujie Su,Yanjun Yang,Junhua Li
In this study, alginate/κ-carrageenan hydrogel beads were developed to encapsulate and release egg yolk immunoglobulin Y (IgY) under simulated gastrointestinal conditions. The effects of κ-carrageenan concentration on the encapsulation and loading efficiency of the hydrogel beads was assessed, as well as on their swelling and release behavior in a simulated human gut. An κ-carrageenan concentration of 0.30% gave the optimum encapsulation and loading efficiency. After incubation in simulated gastric fluids, there was no loss in IgY activity when it was encapsulated within alginate/carrageenan beads but around 35% loss when it was encapsulated in pure alginate beads. This effect was attributed to a stronger electrostatic attraction and a more closely packed biopolymer network formed within the composite beads, based on measurements of their electrical and microstructural properties. The composite beads were resistant to swelling in the stomach phase, but not in the intestine phase. The kinetics of swelling under simulated gastric conditions was found to follow Schott's second-order model. The gastrointestinal digestion of the IgY was slower in the composite beads than in the pure alginate beads. The release kinetics of IgY from the composite beads was fitted by an irregular release model. Our results suggest that alginate/κ-carrageenan composite beads are efficient carriers for the delivery systems of hydrophilic immunoglobins.
研究领域:
食品科学
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生物材料学
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DOI
高影响因子
Chinese Herbal Medicines
·
2022
·
IF 0
Xia Gao,Jiajia Wang,Xialin Chen,Shanli Wang,Chaojie Huang,Quanchang Zhang,Liang Cao,Zhenzhong Wang,Wei Xiao
Objective To elucidate the anti-inflammatory mechanism of Reduning Injection (RDN) by analyzing the potential biomarkers and metabolic pathways of the carrageenan-induced inflammatory model from the overall metabolic level. Methods Rat inflammatory model was established by carrageenan. UPLC-Q-TOF/MS was used to detect and analyze changes of endogenous metabolites in the serum and urine of carrageenan-induced inflammatory rats. Combined with multivariate analysis and databases analysis, inflammatory-related potential biomarkers were screened and identified to analyze possible metabolic pathways. The reliability and biological significance of these biomarkers was verified by metabolic network analysis and correlation analysis with pharmacodynamic indicators. Results A total of 16 potential biomarkers were screened and identified by multivariate analysis and metabolite databases, among which 13 species could be adjusted by RDN. The metabolism pathway analysis revealed that histidine metabolism, sphingolipid metabolism, and tyrosine metabolism were greatly disturbed. Their biomarkers involved urocanic acid, sphingosine, and norepinephrine, all of which showed a callback trend after RDN treatment. The three biomarkers had a certain correlation with some known inflammatory-related small molecules (histamine, arachidonic acid, Leukotriene B4, and PGE 2 ) and pharmacodynamic indicators (IL-6, IL-1β, PGE 2 and TNF-α), which indicated that the selected biomarkers had certain reliability and biological significance. Conclusion RDN has a good regulation of the metabolic disorder of endogenous components in carrageenan-induced inflammatory rats. And its anti-inflammatory mechanism is mainly related to the regulation of amino acid and lipid metabolism. This research method is conducive to the interpretation of the overall pharmacological mechanism of Chinese medicine.
研究领域:
药理学
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炎症研究
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代谢组学
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中药
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DOI
Food & Function
·
2021
·Vol. 12
·期 12
·5407-5416
·
IF 5.396
Shi-Qi Xu,Yi-Tong Han,Jia-Nan Yan,Xin-Yu Jiang,Yi-Nan Du,Hai-Tao Wu
In this paper, some cationic dipeptides from scallop (Patinopecten yessoensis) male gonads (SMGs), which can synergistically gel with ι-carrageenan (ι-C), were screened by the in silico approach. Fourteen protein sequences of SMGs were obtained by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and nano liquid chromatography-mass spectrometry/mass spectrometry (nanoLC-MS/MS) analysis and were then hydrolyzed via in silico simulation. A total of 414 sequences were obtained with 56 duplicates, half of which were positively charged at pH 7. Among the cation sequences, 171 had good water solubility, including two amino acids (Lys and Arg). The molecular weight analysis of the cationic water-soluble sequences showed that 0.2–0.3 kDa accounted for the highest proportion. Based on the obvious synergistic effect of Lys and ι-C, 11 Lys-containing dipeptides, including Ser-Lys (SK), Thr-Lys (TK), Trp-Lys (WK), Ala-Lys (AK), Leu-Lys (LK), Gly-Lys (GK), Val-Lys (VK), Cys-Lys (CK), Asn-Lys (NK), Phe-Lys (FK), and Met-Lys (MK), were finally screened out to study gelation with ι-C. It was found that the dipeptides/ι-C formed firm gels except WK/ι-C. The values of the storage modulus (G′) of 11 dipeptides/ι-C were investigated by a rheometer. The G′ of 8 dipeptides/ι-C was higher than 1000 Pa. These results indicated that the in silico-screened dipeptides from SMGs can form composite gels with ι-C, which can be used for the design and development of functional hydrogels.
研究领域:
食品科学
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生物化学
·
材料科学
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DOI
PubMed
高影响因子
LANGMUIR
·
2025
·
IF 3.9
Liang Zhao,Tuo Yang,Wentao Li,Xuewei Zhang,Qizhong Shi,Xin Feng,Yameng Wang,Xiafei Li,Wenbin Li
Extracorporeal circulation often causes mechanical damage to erythrocytes, which release hemoglobin into the plasma, resulting in postsurgical hazard, so lowering the free hemoglobin (f-Hb) level in the blood is important. In this study, based on the good biocompatibility of chitosan/κ-carrageenan and the adsorption effect of carbon nitride, a chitosan/κ-carrageenan/carbon nitride (CS/κ-Car/C3N4) hydrogel was prepared through the charge electrostatic interaction between polysaccharides. The incorporation of C3N4 enhanced both the adsorptive capacity and the stability of the hydrogel. Specifically, the CS/κ-Car/C3N4-0.4% hydrogel exhibited an adsorption amount of 185.79 mg/g for human hemoglobin, approximately twice that of the CS/κ-Car hydrogel. The CS/κ-Car/C3N4 hydrogel also showed a significantly lower breakage and degradation rate. In addition, the CS/κ-Car/C3N4 hydrogel did not induce hemolysis, complement activation, or coagulate when in contact with blood and showed good blood compatibility. Furthermore, in vivo studies in rabbits revealed that the CS/κ-Car/C3N4 hydrogel significantly reduced elevated f-Hb levels to near-normal concentrations postcirculation, without inducing any severe adverse effects on the subjects. This investigation has elucidated that the CS/κ-Car/C3N4 hydrogel possesses superior adsorptive efficacy and biocompatibility, effectively clearing f-Hb in vivo. Consequently, the findings of this study offer novel insights into the design paradigm for adsorbents intended for blood circulation applications.
研究领域:
生物材料学
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医学工程
·
血液净化技术
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）