FOOD CHEMISTRY
·
2024
·
IF 8.5
Wenhui Ma,Wei Yuan,Xianqi Yao,Miao Chen,Wei Wang,Weizhong Jin,Junrong Huang,Yungang Cao
This study investigated the effects of sodium pyrophosphate (SPP) and catechin (C) on the in vitro enzymatic digestion of oxidatively damaged myofibrillar protein (MP) gel. The results indicated that SPP increased the β-sheet content and the gastric digestibility of the MP gel, while C hindered the transition from α-helix to β-sheet structure, leading to decreased digestibility. Notably, neither compound significantly affected intestinal digestibility. Furthermore, SPP and C significantly enhanced the antioxidant activity of MP gel digestion products. Notably, their synergistic hydrolysis products, simulating both gastric and gastrointestinal stages, chelated 91.4 % and 89.1 % of Fe 2+ and scavenged 59.4 % and 77.6 % of hydroxyl radicals, respectively. Moreover, the final digestion products of the MP gel treated with SPP and C exhibited the highest content of negatively charged amino acids and absolute Zeta potential values. Overall, this study demonstrated that incorporating SPP and C could positively impact the digestion of oxidatively damaged MP gels.
研究领域:
食品科学
·
生物化学
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DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2024
·
IF 8.2
Xiang Dong,Wenying Li,Changzhu Li,Otobong Donald Akan,Chancan Liao,Jie Cao,Lin Zhang
Lead (Pb) affects gene transcription, metabolite biosynthesis and growth in plants. The tung tree ( Vernicia fordii ) is highly adaptive to adversity, whereas the mechanisms underlying its response to Pb remain uncertain. In this work, transcriptomic and metabolomic analyses were employed to study tung trees under Pb stress. The results showed that the biomass of tung seedlings decreased with increasing Pb doses, and excessive Pb doses resulted in leaf wilting, root rot, and disruption of Pb homeostasis. Under non-excessive Pb stress, a significant change in the expression patterns of flavonoid biosynthesis genes was observed in the roots of tung seedlings, leading to changes in the accumulation of flavonoids in the roots, especially the upregulation of catechins, which can chelate Pb and reduce its toxicity in plants. In addition, Pb-stressed roots showed a large accumulation of VfWRKY55 , VfWRKY75 , and VfLRR1 transcripts, which were shown to be involved in the flavonoid biosynthesis pathway by gene module analysis. Overexpression of VfWRKY55 , VfWRKY75 , and VfLRR1 significantly increased catechin concentrations in tung roots, respectively. These data indicate that Pb stress-induced changes in the expression patterns of those genes regulate the accumulation of catechins. Our findings will help to clarify the molecular mechanism of Pb response in plants.
研究领域:
植物生理学
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分子生物学
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环境科学
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2025
·
IF 2.6
Yunfan She,Chenxin Dong,Yumin Leng,Zhengbo Chen
The catechins in tea, as promoters of human health, have aroused widespread attention. Herein, a dual-signal mode (colorimetric and fluorescence) sensor array for catechin species fingerprinting was built based on PtNi bunched nanoparticles (PtNi-BNPs)-o-phenylenediamine (OPD)-H2O2 system. As we know, PtNi-BNPs catalyze the reaction between OPD and H2O2 to produce oxidized OPD (oxOPD) with both colorimetric (yellow) and fluorescent properties. As an antioxidant, catechins can inhibit the above reaction, resulting in a lighter color and reduced fluorescence intensity in their solution. Due to the varying inhibitory abilities of different catechins on their reactions, they produce unique colorimetric-fluorescence response patterns. These diverse responses were recorded and processed by linear discriminant analysis (LDA) and six catechin species were identified by the sensor array. Furthermore, our work provides a convenient method for simultaneous differentiation of catechins in mixtures and real samples. Such a sensor array with minimal sensor element and abundant signal outputs greatly saves cost and time, providing a powerful tool for the discrimination and assessment of catechins.
研究领域:
分析化学
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食品科学
·
生物医学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2018
·
IF 4.946
Yuting Li,Lin Li,Marianne N. Lund,Bing Li,Yi Hu,Xia Zhang
(−)-Epicatechin (EC) and (−)-epigallocatechin gallate (EGCG) (0.001–5%) were found to reduce N ε -(carboxymethyl) lysine (CML) concentrations by up to 0.80 ± 0.11 mM and 0.85 ± 0.07 mM, respectively, as quantified by HPLC-MS during heating at 80 °C and 100 °C in a glucose-lysine model system at pH 5, 7 and 8, which mimic different food processing conditions. With the addition of 1 mM EC, the free CML concentration in coconut milk was reduced to 5.7 ± 0.1 μg/mL (∼2.8 × 10 −2 mM), which was significantly lower than that in unheated coconut milk (6.1 ± 0.2 μg/mL (∼3.0 × 10 −2 mM), p < 0.05) indicating that the free CML may be eliminated by the addition of EC in coconut milk. Products of EC/EGCG quinones reacted with CML by a Michael addition or Schiff base addition in model systems were tentatively identified by UPLC-ESI-MS/MS.
研究领域:
食品科学
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生物化学
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产品关联
DOI
PubMed
高影响因子
Toxics
·
2023
·Vol. 11
·期 9
·799
·
IF 4.6
Hangwei Wang,Zhanren Liu,Shaojie Liu,Ruoru Yang,Yifei Wang,Yiying Gu,Min Wu,Ruihua Dong,Bo Chen
This study aimed to assess the therapeutic efficacy of catechin against experimentally induced kidney stones resulting from co-exposure to melamine (MEL) and cyanuric acid (CYA) in male Sprague–Dawley rats. To induce nephrolithiasis, a combination of MEL and CYA (1:1 ratio, each at a dose of 31.5 mg/kg bw/day) was administered to the rats for 28 consecutive days. After nephrolithiasis was successfully induced, the rats were randomly divided into two groups: a treatment group and a sham group. The treatment group was given a daily oral dose of 50 mg/kg of catechin for 28 days, while the sham group received no intervention. Urine and blood samples were collected throughout the treatment period, and kidney samples were taken on day 28. Our findings demonstrated that treatment with catechin significantly reduced crystal deposition and pathological damage in the rats from nephrolithiasis. Additionally, renal injury markers were significantly decreased in the treatment group compared to the sham group. These findings suggest that catechin has potential therapeutic benefits in treating nephrolithiasis induced by co-exposure to MEL and CYA.Keywords:melamine;cyanuric acid;nephrolithiasis;catechin
研究领域:
毒理学
·
药理学
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产品关联
DOI
PubMed
高影响因子
POLYMER ENGINEERING AND SCIENCE
·
2026
·
IF 3.6
Lei Nie,Letian Yan,Yaling Deng,Yanfang Sun,Guohua Jiang
The hydrogel bio-adhesive has demonstrated outstanding potential in regenerative wound care. Here, we prepared borate-crosslinked bioadhesive hydrogels (PPGB) composed of dopamine-conjugated gelatin (Gel-DA), polyvinyl alcohol (PVA), and catechin acid. Scanning electron microscopy revealed an interconnected porous microstructure within the PPGB matrix. Rheological characterization confirmed typical viscoelastic solid behavior, favorable injectability, and excellent self-healing capability, the latter of which was also supported by macroscopic observations. Antioxidant activity was validated through ABTS and DPPH radical scavenging assays. The PPGB adhesives exhibited strong adhesion to various material surfaces and moist organic tissues. Antibacterial testing demonstrated efficient activity against the typical Gram-positive and Gram-negative bacteria. Biocompatibility evaluation using CCK-8 assays and DAPI/phalloidin staining of NIH 3 T3 fibroblasts exposed to hydrogel extracts indicated good cytocompatibility. Furthermore, a scratch wound healing assay confirmed enhanced cell migration. Collectively, these findings present a straightforward strategy for engineering multifunctional hydrogel bio-adhesives with potential for biomedical applications. Highlights Dopamine-conjugated gelatin was synthesized. Borate-crosslinked PPGB hydrogels were fabricated as bioadhesives. PPGB hydrogels displayed good antioxidant activities. PPGB hydrogel bioadhesives exhibited strong adhesion to various materials and organs. PPGB hydrogels exhibit suitable cytocompatibility and cell-promoted migration.
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2026
·
IF 10.4
Huanhuan Liu,Yi Zhao,Boyu Cheng,Fengyi Guo,Xiuqing Yao,Jie Gao,Dima Atehli,Tianyu Qi,Ji Kang,Steve W. Cui,Yuanlin Sun,Qingbin Guo
DBD plasma enables catechin grafting on CAX without disrupting its skeleton. • ECG shows the highest grafting yield among four catechin monomers. • ECG moieties mainly anchor to the inner xylan backbone of CAX. • Catechin grafting partly restores plasma-condensed CAX chain expansion. • Catechin grafting shifts CAX sheets into porous fibrous networks. A green, non-thermal dielectric barrier discharge (DBD) plasma protocol was established to covalently graft four structurally distinct catechin monomers, epicatechin, epicatechin gallate (ECG), epigallocatechin, and epigallocatechin gallate, onto corn bran arabinoxylan (CAX). Among the synthesized conjugates, ECG-CAX showed the highest grafting yield (72.66 mg/g). Fourier transform infrared spectrum (FT-IR) and ultraviolet-visible (UV–vis) spectroscopy analyses verified phenolic incorporation while preserving the primary polysaccharide skeleton. Two-dimensional nuclear magnetic resonance (2D-NMR), supported by gradient acid hydrolysis, indicated a free-radical-mediated, heterogeneous multi-site conjugation network, with the B- and D-rings of ECG predominantly anchored to the inner xylan backbone. Plasma treatment contracted native CAX from flexible random coils into compact coil conformations, whereas phenolic grafting partially reversed this transition by introducing steric hindrance and promoting chain re-expansion into semiflexible coils. This conformational evolution reduced apparent viscosity and shifted the micromorphology from lamellar sheets to interconnected porous fibrous networks, providing mechanistic insight into plasma-mediated polysaccharide–polyphenol conjugation. Download: Download high-res image (189KB) Download: Download full-size image
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DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
·
IF 7.7
Haowei Lv,Huiling Xu,Enbo Xu,Zhengyu Jin,Haibo Zhao,Chao Yuan,Meng Zhao,Zhengzong Wu,Deyun He,Bo Cui
The hydrophilic and low mechanical properties limited the application of starch-based films. In this work, a hydrophobic starch-based nanofiber mat was first successfully prepared from aqueous solution at room temperature by using electrospinning and glutaraldehyde (GTA) vapor phase crosslinking techniques for active packaging applications. Catechin (CAT) was immobilized in the nanofibers by electrospinning, resulting in higher thermal stability (T dmax = 315.23 °C), antioxidant (DPPH scavenging activity = 94.31 ± 2.70 %) and antimicrobial (inhibition zone diameter = 15.6 ± 0.3 mm) of the fibers, which further demonstrated hydrogen bonding and electrostatic interaction between CAT and fibers. Nanofibers after GTA vapor phase crosslinking exhibited enhanced hydrophobicity (water contact angle: 15.6 ± 1.5° → 93.5 ± 2.3°) and mechanical properties (tensile strength: 1.82 ± 0.06 MPa → 7.64 ± 0.24 MPa, elastic modulus: 19.35 ± 0.63 MPa → 45.34 ± 0.51 MPa). The results demonstrated that preparation of starch-based electrospun nanofiber mats in aqueous system at room temperature overcame the challenges of organic solvent pollution and thermosensitive material encapsulation, while GTA vapor phase crosslinking technique improved the hydrophobicity and mechanical properties of nanofiber mats, which facilitated the application of starch-based materials in the field of packaging.
研究领域:
材料科学
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食品科学
·
纳米技术
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产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2024
·
IF 4.8
Jun Lv,Chang Liu,Pan-Pan Tang,Jun-Xiang Liu,Rana Muhammad Aadil,Jun-Hu Cheng,Shahzad Hussain,Xiu-Bin Liu,Zhi-Wei Liu
The application of cold plasma (CP) treatment was investigated for its effectiveness in promoting the covalent grafting of β-lactoglobulin (β-LG) and epigallo-catechin 3 -gallate (EGCG), as well as improving the antioxidant capacity of β-LG and eliminating its antigenicity. The results indicated that CP treatment significantly enhanced the covalent grafting of β-LG and EGCG. The maximum EGCG content in β-LG-EGCG complexes reached 47.08 ± 0.58 mg/g (with 45 s treatment), as confirmed by SDS-PAGE analysis and FTIR spectroscopy. Additionally, LC-MS/MS identifies two binding sites (T76 and K77) of EGCG in the β-LG-EGCG conjugates. After 60 s treatments, there was a significant reduction in the IgG binding capacity of β-LG-EGCG conjugates (29.97 ± 0.05%). Further analysis suggested that the change in the conformational epitopes of β-LG, due to the conjugating with EGCG, was likely the main factor responsible for the reduced binding Immunoglobulin G (IgG) capacity. The maximum antioxidant activities of β-LG-EGCG conjugates were found to be 974.14 ± 1.94 μmol TE g −1 for ABTS and 748.48 ± 9.66 μmol TE g −1 for DPPH). These values represent an eightfold increase in DPPH activity and a twofold increase in ABTS compared to the control sample. This study demonstrated that the CP treatment is highly effective technology for facilitating the covalent grafting of polyphenols to proteins, thereby enhancing their antioxidant properties and eliminate their antigenicity.
研究领域:
食品科学
·
生物化学
·
免疫学
·
等离子体技术
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产品关联
DOI
高影响因子
Foods
·
2025
·Vol. 14
·期 19
·3360
·
IF 5.1
Yaqi Liu,Zhenli Cai,Yao Fan,Xingcai Wang,Meixia Wu,Haiyan Fu,Yuanbin She
Aimed at addressing the increasingly serious problem of adulteration in Xihu Longjing, a catechin-targeted nano-enhanced visual and fluorescent dual-mode sensor array was constructed by nano porphyrins and quantum dots (QDs) for the precise analysis of Xihu Longjing from adjacent origins. This sensor array realizes the quantitative analysis of catechin enantiomers in Xihu Longjing through the selective combination of sensing units. It can accurately identify adjacent Xihu Longjing teas with different grades and storage times and can precisely detect samples with a low proportion of adulteration. At the same time, the flavor quality and antioxidant performance of Xihu Longjing tea can also be quantitatively evaluated. The dual-mode sensor array design proposed in this study provides a new idea for detecting minor differences in food authenticity and has significant application value for quality control in the tea industry.
研究领域:
食品科学
·
分析化学
·
纳米生物技术
·
茶叶质量控制
·
分子识别
·
传感技术
·
天然产物化学
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 192
·期 1
·1-14
·
IF 5.3
Wang Yilin,Zhao Zixia,Wang Yuefan,Liu Zhifang,Chen Leyao,Qi Jin,Xie Yixi,Zhao Pengcheng,Fei Junjie
GO/Co-MOF/PPy-350 (GPC-350) was synthesized by in situ growth of ultrafine Co-MOF on graphene oxide (GO), followed by encapsulation with polypyrrole (PPy) and calcination at 350.0℃. Meanwhile, MoS 2 -MWCNTs (MoS 2 -CNTs) were produced via the in situ synthesis of MoS 2 within multi-walled carbon nanotubes (MWCNTs). The electrochemically superior GPC-350/MoS 2 -CNTs nanocomposite was then achieved by combining GPC-350 with MoS 2 -CNTs. The polypyrrole encapsulation serves to protect the ultrafine Co-MOF, preventing its degradation during the calcination process. The linear detection range of the GPC-350/MoS 2 -CNTs/GCE sensor for the determination of catechin (CA) in phosphate buffered saline (PBS) was from 5.0 to 1800.0 nM with a limit of detection of 1.78 nM. In addition, the materials were characterized using SEM, EDX, TEM, XRD, EIS, XPS, FTIR, and Raman. These results indicate that the synthesis of GPC-350/MoS 2 -CNTs nanocomposites is successful and CA in beverages samples can be effectively detected using electrochemical sensors. Additionally, the reaction mechanism of CA was explored through cyclic voltammetry. The application of GPC-350/MoS 2 -CNTs nanocomposites in sensor technology offers innovative approaches for the ultrasensitive detection of flavonoids. Graphical abstract
研究领域:
材料科学
·
分析化学
·
食品科学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2009
·
IF 2.696
Yulong Chen,Yueming Jiang,Jun Duan,John Shi,Sophia Xue,Yukio Kakuda
The variations in the contents of catechins and phenolic compounds in relation to the quality scores (QS) and taste scores (TS) in the ‘ Huang Zhi Xiang ’ Oolong tea grown in different seasons and at different altitudes were determined. The study demonstrated that the contents of (−)-epigallocatechin gallate (EGCG), catechin gallate (CG), and total catechins (TC) in the Oolong teas grown at a high altitude were significantly higher than those grown at a low altitude. Furthermore, the contents of EGCG, CG, and TC in the autumn tea were significantly higher than those from the spring tea grown at a low altitude. However, no significant differences in the contents of EGCG, CG, and TC were observed between the spring and autumn teas grown at a high altitude. In addition, the contents of (−)-epigallocatechin (EGC), (+)-catechin (C), (−)-gallocatechin (GC), and simple catechins (SC) in the spring tea leaves were significantly higher than those in the autumn tea leaves grown at a low altitude, but they were not significantly different from those in the autumn tea leaves grown at a high altitude. The contents of EGCG, CG, and TC in the Oolong teas were positively and significantly correlated to the taste score (TS) and quality score (QS) of the Oolong tea. Thus, the contents of EGCG, CG, and TC could be the important quality parameters of the ‘ Huang Zhi Xiang ’ Oolong tea. This work suggested that the production of the ‘ Huang Zhi Xiang ’ Oolong tea grown at low altitudes should utilize the autumn tea leaves while the production of Oolong tea grown at high altitudes can use both the spring and autumn tea leaves.
研究领域:
植物学
·
食品科学
·
农业科学
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DOI
高影响因子
危险品化学品经营许可证（带存储）