ANALYTICA CHIMICA ACTA
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2025
·
IF 6
Lu Lin,Mengmeng Lv,Jiangtao Ren,Jin Wang,Erkang Wang
Background G-quadruplex DNAzymes, known for their peroxidase-like activity and ease of rational design, have emerged as promising tools in biosensing applications. Despite the fundamental importance of structural topology, investigations into how the split mode of intramolecular split G-quadruplex (IntrSG)-based DNAzymes modulates their catalytic properties, as well as their implications for the development of DNAzyme-based probes, remain scarce. In this work, eleven IntrSG strands of different split modes were engineered with a classical parallel G-quadruplex sequence ( PW17 ). The catalytic activity of IntrSG-hemin mixtures was systematically investigated via various techniques. Results Most IntrSG strands incubated with hemin exhibited apparent catalytic activity, which was influenced by split modes and hybridization of inserted spacer. Interestingly, the mixture of hemin and IntrSG of split mode 5:7 (IntrSG(5:7)) exhibited drastic catalytic activity response after hybridization between its spacer and target, and the underlying mechanism was disclosed, which was hybridization-facilitated G-quadruplex assembling and catalytic enhancement. The IntrSG(5:7)-hemin system can be utilized as an effective “signal-on” signal transducer, owing to its simpleness and universality. Subsequently, influence of sequence context changes on the catalytic response was explored by extending and truncating the target sequence. Ignorable effect of protruding DNA was validated, and robust hybridization was critical for catalytic responses, which were unrelated to hybridization location. Finally, an IntrSG(5:7)-based catalytic probe was employed for quantitative analysis of miRNA ( Let-7a ), and favorable performance was achieved. Significance Taken together, a mechanistic framework for topological control of G-quadruplex DNAzyme catalysis was established, highlighting the potential of IntrSG as a versatile platform for biosensing and molecular diagnostics.
研究领域:
生物化学
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分子诊断学
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生物传感
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核酸工程
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DNA纳米技术
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PubMed
高影响因子
TALANTA
·
2025
·
IF 6.1
Wenchao Ran,Chi Xu,Xiaomei Pan,Xiaojun Huang,Chong Wang,Xianggui Chen,Jie Tang,Imran Mahmood Khan,Yukun Huang
Ofloxacin, a synthetic antimicrobial drug, exhibits significant therapeutic potential in combating various bacterial infections. The exploration of ofloxacin's antimicrobial properties and the unique catalytic activity of G-triplex(G3) DNAzymes demonstrate the continuous efforts to develop cutting-edge biosensors for bacterial detection. G3 outperforms G4 due to a short, easily generated and dissociated sequence, which allows G3 multimeric sequences to fold more accurately than G4 ones of equivalent length, resulting in higher activity for the formed G3/hemin. This study constructed a novel colorimetric DNAzyme biosensor based on G3/hemin for ofloxacin in food. Aptamer-functionalized Fe 3 O 4 magnetic nanoparticles acted as magnetic separation and detection probes, while G3/hemin served as DNAzyme. Under the optimum condition, the presence of ofloxacin initiated the catalytic oxidation to yield a colored product to quantify the ofloxacin concentration with the linear range of 50–300 nmol/L. Under the optimized conditions, a limit of detection of 15.25 nmol/L was obtained by directly detecting the fluorescent signals, which shows obvious superiority over the previously reported methods. This method offers the advantages of simplicity, rapid detection, and high recovery rates in standardization experiments involving actual samples such as beef jerky and ham sausage, paving the way for its widespread implementation in the fields of biomedical diagnostics, environmental monitoring, and food safety.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Xu-Mei Zhang,Man Zhang,Ning-Ning Xu,Shu-Juan Zheng,Ni Cheng
Chronic cutaneous wounds present a significant challenge for healthcare providers globally, with the risk of bacterial infections emerging as a particularly concerning issue. There is an increasing need to employ a combination of diverse antibacterial strategies to address infections comprehensively in chronic wounds. This study introduces a highly efficient antibacterial platform that encapsulates the NO precursor (BNN6) into β-cyclodextrin-modified hemin-bearing polydopamine nanoparticles called NO/CHPDA. These nanoparticles are seamlessly integrated into a hydrogel composite comprised of L-arginine grafted chitosan (Arg-CS) and oxide dextrans (oDex). The amalgamation of photothermal therapy (PTT), chemodynamic therapy (CDT), and nitric oxide (NO) antibacterial strategies within the NO/CHPDA@Arg-CS/oDex nanocomposite hydrogel demonstrates a synergistic and highly effective capacity to eradicate bacteria and accelerate the wound healing process in vivo . Remarkably, this nanocomposite hydrogel maintains excellent biocompatibility and induces minimal side effects. The resulting nanocomposite hydrogel represents a promising therapeutic solution for treating bacterial infections in wound healing applications.
研究领域:
生物材料学
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医学
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药剂学
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PubMed
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 9
·1-10
·
IF 5.3
Zhao Fan,Liu Yunxi,Lan Jingyue
A simple and efficient dual-signal electrochemical sensor was designed for glyphosate (GLYP) determination based on the one-step electro-synthesized Cu-Hemin MOFs/CNTs nanocrystals. Cu-Hemin MOFs/CNTs were directly modified on the electrode through electrodeposition, avoiding complicated synthesis and modification processes. The incorporation of CNTs greatly boosted the conductivity of Cu-Hemin MOFs and the sensitivity of the electrochemical sensor. Cu active sites in Cu-Hemin MOFs were converted to CuCl, allowing the specific detection of GLYP with the turn of CuCl into non-electroactive Cu-GLYP. Meanwhile, GLYP showed highly effective inhibition effect on the inherent peroxidase-like activity of Cu-Hemin MOFs, therefore generating the second electrochemical signal with Cu-Hemin MOFs-catalyzed o -phenylenediamine ( o -PD) + H 2 O 2 system. The Cu-Hemin MOFs/CNTs based sensor with two electrochemical signals showed good linearities of 1.0 × 10 −10 M − 3.0 × 10 −6 M and 1.0 × 10 −10 M − 5.0 × 10 −5 M, with detection limits of 5.17 × 10 −12 M and 6.81 × 10 −12 M for the CuCl signal based assay and nanozyme catalyzed o -PD + H 2 O 2 procedure, respectively. This simple and robust dual-signal sensor with excellent selectivity, accuracy, and stability allowed GLYP quantification in real samples, highlighting the potential application of this approach for food and environmental monitoring. Graphical Abstract
研究领域:
分析化学
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环境科学
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食品科学
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DOI
PubMed
高影响因子
RSC Advances
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2012
·Vol. 2
·期 18
·7140-7145
·
IF 0
Yunlei Zhou,Mo Wang,Xiaomeng Meng,Huanshun Yin,Shiyun Ai
A novel signal amplified electrochemical microRNA (miRNA) biosensor was developed here using hemin-G-quadruplex as signal unit. A hairpin structure DNA probe (43 bases) was immobilized on the gold nanoparticles (AuNPs) modified gold electrode surface with the segment at its 3′-end complementary to miRNA-21 (22 bases) and the segment at its 5′-end complementary to capture DNA (21 bases). Upon hybridization with the target miRNA-21, the hairpin structure was unfolded, which was further hybridized with capture DNA loaded on AuNPs. The AuNP contained two kinds of DNA; one was complementary to the hairpin structure DNA probe, while the other was aptamer for hemin. The electrochemical signal of hemin self-assembled in the center of G-quadruplex structure of the aptamer was measured using chronoamperometry. The miRNA-21 target was analyzed with a detection limit of 3.96 pM. Using isolated total RNA from human gastric carcinoma BGC-823 cells, human breast adenocarcinoma MCF-7 cells, human hepatocarcinoma HepG2 cells and human colon cancer HT-29 cells, the assay detected specifically miRNA-21 and over-expression of oncogene miRNA-21 was demonstrated.
研究领域:
生物化学
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生物技术
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分析化学
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癌症研究
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DOI
RSC Advances
·
2014
·Vol. 4
·期 84
·44614-44620
·
IF 3.708
Fengmin Qiao,Weijie Shi,Jing Dong,Wei Lv,Shiyun Ai
Hemin has been successfully modified onto the surface of CuAl layered double hydroxide nanosheets by a simple coprecipitation process, which afforded a hemin modified CuAl layered double hydroxide (H-LDH) hybrid functional material that exhibited protective effects against the harmful ONOO−. The obtained products were characterized by X-ray powder diffraction, scanning electron microscopy, transmission electron microscopy and Fourier transform infrared spectroscopy, which showed that the samples had hexagonal symmetry structure with a mean lateral size of 1 μm, on the surface of which were adsorbed round particles with a diameter of about 300 nm. A detailed inhibition study on ONOO−-mediated nitration reactions indicated that the interaction between hemin and LDHs results in a synergistic effect, which leads to an efficient reduction of ONOO− to nitrate. The present study suggests that the H-LDHs had an efficient ONOO− scavenging ability, and may well be a potent ONOO− scavenger for protection of the cellular defense activity against ONOO− involved diseases.
研究领域:
材料科学
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生物化学
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DOI
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
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2022
·
IF 4.598
Jie Wei,Yumeng Li,Qingyang Si,Qiao Xiao,Qingmin Chen,Tianhui Jiao,Quansheng Chen,Xiaomei Chen
A label-free, sensitive and reliable electrochemical sensor based on hemin/G-quadruplex (G4) DNAzyme was engineered to determine adenosine triphosphate (ATP) in fish. The nucleic acid sequence (DNA1) including the G4 sequence subunit and anti-ATP aptamer segment is anchored on Au electrodes. The nucleic acid sequence (DNA2) is composed of another G4 sequence fragment and anti-ATP aptamer subunit. The probes DNA1 and DNA2 self-assemble into the active hemin/G4 DNAzyme structure on Au electrodes upon exposure to the target ATP. The determination of the target ATP relied on an increase in the catalytic currents occurring on DNAzyme-anchored electrode via the electrochemical reduction of H 2 O 2 . The proposed strategy enables high-performance and specified determination of the target ATP with a detection limit of 0.04 μmol/L. Specifically, the proposed ATP strategy was further implemented to determine ATP in microorganisms in carp samples. Compared with the classical total viable count (TVC) assay, the proposed ATP assay was feasible to assess the freshness of carp, which expands the applications of hemin/G4 DNAzyme and holds promising potential for freshness assessment in food safety.
研究领域:
分析化学
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食品科学
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电化学
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生物传感器
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高影响因子
JOURNAL OF ALLOYS AND COMPOUNDS
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2021
·
IF 5.316
Songmei Li,Yimin Yu,Xiaoyan Sun,Xifeng Ding,Huafang Yang,Shibing Pan,Lixi Wang,Qitu Zhang
Ni(OH) 2 has been acknowledged as a promising oxygen evolution reaction (OER) electrocatalyst owing to its good electrochemical activity and low cost. However, application of the pristine Ni(OH) 2 is limited by the poor conductivity and low exposure of the active sites. Herein, a [email protected] (OH) 2 composite is prepared through a hydrothermal reaction. Heat treatment temperature proves to have a great influence on the electrocatalytic activity. In 0.1 M KOH electrolyte, the composite treated at 175 °C shows an overpotential of only 287 mV to reach the current density of 10 mA·cm −2 . Besides, the low Tafel slope (42 mV·dec −1 ) and superior stability (15 h at 10 mA·cm −2 ) also confirm the composite as an excellent OER electrocatalyst. The brilliant activity and stability toward OER can be attributed to the promoted coupling between Ni and Fe centers and the improved conductivity due to the 2D 18 p-electrons ring conjugate structure of hemin. Besides, effective hydrogen bonds between Ni(OH) 2 and hemin hinder the aggregation of Ni(OH) 2 , which ensures the catalyst enormous accessible active sites. This work offers a new method for the modification of Ni(OH) 2 as OER electrocatalysts. And the application of hemin also serves as a successful case for the use of biomass materials in OER electrocatalysis .
研究领域:
电化学
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材料科学
·
催化
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DOI
高影响因子
Polymers
·
2022
·Vol. 14
·期 23
·5135
·
IF 4.967
Xu Han,Yun Tao,Chao Xu,Yicong Deng,Zisen Meng,Zhenhao Dou,Peng Wang,Quan Feng
Hemin possesses great potential in eliminating organic pollutants due to its mild reaction condition, light-harvesting efficiency, and environmental friendliness. However, it has drawbacks such as being easy to aggregate and hard to recycle, and poor stability should be improved in practical application. Herein, the subject developed an electrospinning approach to enable the hemin particulates to be immobilized onto polyacrylonitrile (PAN) nanofibers stably. Hydrogen peroxide (H2O2) was adopted as an oxidant in the system to simulate the enzymatic catalysis of hemin in an organism. Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), UV-Vis diffuse reflection spectroscopy (DRS), and electron spin resonance spectroscopy (ESR) analysis was employed to discuss the morphology, structure, and mechanism of the prepared n-hemin/PAN nanocomposite membranes, and 0.02 mmol L−1of the rhodamine B (RhB) removal activity in different conditions was also verified with these membranes. The kinetic studies showed that n-hemin/PAN nanocomposite membranes maintained excellent properties both in adsorption and degradation. Around 42% RhB could be adsorbed in the dark, while 91% RhB decolorized under xenon lamp irradiation in 110 min, suggesting the catalytic performance of n-hemin/PAN was greatly driven by light irradiation. Differing from the axial coordinated hemin complexes, n-hemin/PAN would catalyze hydrogen peroxide into •OH radicals rather than •OOH and high-valent metal-oxo species. This work provides an effective way to support hemin as nanocomposite membranes, in which the molecular interaction between polymer and hemin made their light adsorption an obvious red shift.Keywords:polyacrylonitrile;hemin;photocatalysis;enzyme-like catalysis;electrospinning
研究领域:
环境科学
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材料科学
·
生物化学
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PubMed
高影响因子
POLYMER
·
2022
·
IF 4.432
Cheng Deng,Hai Yang,Simin Liu,Zhiyong Zhao
Nanomaterials self-assembled by DNA amphiphiles have great potential application in catalysis, bioimaging and biomedicine. Here, we constructed two kinds of DNA-dendrimer amphiphiles including the G-quadruplex-dendrimer hybrid (G20-Den) and single-stranded DNA-dendrimer hybrid (D20-Den) as a control. Both of them self-assembled into nanofibers with a length of several micrometers in MES buffer, and the diameter of G20-Den nanofibers was smaller than that of D20-Den nanofibers due to the compact stacking of G-quadruplex formed in G20-Den hybrids. At the same time, the catalytic properties of G20-Den nanofiber/hemin, as horseradish peroxidase-mimicking DNAzymes, were investigated. Our results showed that G20-Den nanofiber/hemin, as well as the single-stranded G20/hemin system, exhibited higher catalytic peroxidation activity than D20-Den nanofiber/hemin. Furthermore, through simple ultrafiltration, the recycle of these nanofibers can be simply achieved. This research has provided a new solution for the construction of functional DNA nanomaterials.
研究领域:
生物化学
·
纳米医学
·
材料科学
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DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2022
·
IF 8.008
Yun Chen,Dehui Qiu,Xiaobo Zhang,Yuan Liu,Mingpan Cheng,Jianping Lei,Jean-Louis Mergny,Huangxian Ju,Jun Zhou
G-quadruplex/hemin (G4/hemin) DNAzymes are biosensing systems, but their application remains limited by an overall low activity and a rather high level of unwarranted background reactions. Here, these issues were addressed through the rational design of F3T-azaC-hemin, a G4-based construct in which the hemin is covalently linked to the G4 core and its binding site flanked with a nucleotide activator, here d(T-azaC). This design led to a G4-DNAzyme whose performances have been ca. 150-fold increased compared to the parent G4-based system. The utility of F3T-azaC-hemin was demonstrated here through the ultrasensitive chemiluminescent detection of miRNA-221. The limit of detection (LOD) has been decreased to the femtomolar range, making it a new and highly efficient molecular tool in the biosensing technology field.
研究领域:
生物传感技术
·
分子生物学
·
分析化学
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DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2026
·
IF 6.7
Wencheng Xiao,Hang Ao,Nilin Wu,Jun Sun,Wenrui Hu,Yunlong Chen,Jie Wu,Huangxian Ju
Highly sensitive detection of cell secretions at the single-cell level is critical for efficient cell screening and monoclonal antibody (mAb) production. Herein, we present a highly sensitive microfluidic chemiluminescence immunoimaging (μCLII) method for rapid and quantitative screening of high-yield specific hybridoma cells (HSHCs) by detecting single-cell secreted antibodies on a rationally designed lantern-shaped microchamber array chip. This method can effectively capture and isolate single hybridoma cells to in situ detect their secreted antibody by visual CL imaging with a proximity recognition amplification strategy. The proposed strategy can be performed by antibody-triggered proximity hybridization to release a DNA sequence, which initiates a rolling circle amplification reaction to turn on abundant hemin-DNA enzymes for generating strong CL emission of the imidazole-enhanced luminol-H2O2 system. This method shows a detection limit of ∼60 antibodies per chamber, and thus enables a precise sorting of HSHCs within 70 min. Using antiproprotein convertase subtilisin/kexin type 9 mAb (PCSK9-mAb) as a model, all of the cells selected by the μCLII method show high-producing hybridomas with PCSK9-mAb yield more than 80 fg during the screening process, indicating a promising potential of the proposed method for single-cell analysis and mAbs screening.
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