MICROCHEMICAL JOURNAL
·
2026
·
IF 5.2
He Bu,Qingling Zhao,Jie Gao,Zixuan Zhang,Ze Liu,Lanlan Li,Xiaofei Yu,Xiaojing Yang,Zunming Lu,Shuquan Zhang,Xinghua Zhang
Fluorescent carbon dots (CDs) derived from traditional Chinese medicine (TCM) face limitations in water solubility and tunable emission, hindering their biomedical application. To address this, we synthesized baicalin-based CDs and engineered their surface with cetyltrimethylammonium chloride (CTAC) and Cu ions. This sequential modification first shifted the emission from blue to orange by introducing N/O-containing functional groups that reduced the energy gap. Subsequent Cu incorporation created Cu-related energy levels, further narrowing the gap to achieve red emission at 660 nm alongside enhanced solubility. In addition, the resulting Cu/CTAC-CDs served as a selective fluorescent probe for histidine, enabling its sensitive detection in complex matrices such as pig blood and milk. This work provides a viable route to fabricate water-soluble, emission-tunable TCM-derived CDs and offers fundamental insights into surface-state-controlled luminescence, advancing their development for biomedical applications.
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高影响因子
MACROMOLECULES
·
2025
·
IF 5.2
Xubo Fan,Xiyu Wang,Xiaoyue Xu,Qing Zheng,Xinhua Lu,Yuanli Cai
We herein demonstrate charge-mediated self-coacervation of zwitterionic histidine acrylamide that leads to viscoelasticity-controlled autocatalytic polymerization under photo-RAFT conditions. This monomer assembles into well-defined ion clusters, followed by self-coacervation via liquid–liquid phase separation (LLPS), leading to fluid droplets (pH 2.5) and hardening droplets (pH 7.1). A RAFT chain transfer agent (CTA) acts as a sticker to promote droplet fusion and Ostwald ripening, leading to autocatalytic reactions involving the induction period, fast chain propagation, and kinetically trapped or aged slow reactions, in a monomer cluster polymerization manner, showing salt-bridged proton-feedback capable of acidification of the acidic reaction medium and alkalization of the neutral reaction medium. Hydrogen-bonded complexation of macro-CTA onto monomer coacervates leads to liquid–liquid core–shell droplet reactors at pH 2.5; monomer plasticization can improve coacervate fluidity, leading to a seeded autocatalytic polymerization up to >95% conversion in 11 min. The final ultrathin lamellae exhibit outstanding responsiveness and unprecedented colloidal stability against dilution, pH fluctuation, salting, and long-term storage. These findings highlight pivotal roles of orchestrated associative/repulsive charge interactions for LLPS-mode coacervation and provide a simple guideline to predict viscoelasticity-controlled autocatalytic polymerization with enzyme-like reaction efficacy.
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高影响因子
NUCLEIC ACIDS RESEARCH
·
2022
·Vol. 50
·期 9
·4917-4937
·
IF 19.16
Wang Wenmeng,Qiao Shiyao,Li Guangyue,Cheng Jiahui,Yang Cuicui,Zhong Chen,Stovall Daniel B,Shi Jinming,Teng Chunbo,Li Dangdang,Sui Guangchao
As an oncogenic transcription factor, Yin Yang 1 (YY1) regulates enhancer and promoter connection. However, gaps still exist in understanding how YY1 coordinates coactivators and chromatin enhancer elements to assemble enhancers and super-enhancers. Here, we demonstrate that a histidine cluster in YY1’s transactivation domain is essential for its formation of phase separation condensates, which can be extended to additional proteins. The histidine cluster is also required for YY1-promoted cell proliferation, migration, clonogenicity and tumor growth. YY1-rich nuclear puncta contain coactivators EP300, BRD4, MED1 and active RNA polymerase II, and colocalize with histone markers of gene activation, but not that of repression. Furthermore, YY1 binds to the consensus motifs in the FOXM1 promoter to activate its expression. Wild-type YY1, but not its phase separation defective mutant, connects multiple enhancer elements and the FOXM1 promoter to form an enhancer cluster. Consistently, fluorescent in situ hybridization (FISH) assays reveal the colocalization of YY1 puncta with both the FOXM1 gene locus and its nascent RNA transcript. Overall, this study demonstrates that YY1 activates target gene expression through forming liquid-liquid phase separation condensates to compartmentalize both coactivators and enhancer elements, and the histidine cluster of YY1 plays a determinant role in this regulatory mechanism.
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PubMed
高影响因子
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
·
2024
·
IF 8.1
Heba Emam,Rashid Al Hajri,Nazir Ahmad,Fatma M. Elantabli,M. El-Rabiei,Abdelwahab Hassan,Mohammed Al Abri,Hussein A. Younus
The quest for an efficient and sustainable electrocatalyst for hydrogen evolution reaction (HER) using earth-abundant elements is crucial for cost-effective and environmentally friendly hydrogen production. While many catalysts show good activity and stability in strongly basic conditions, fewer materials are effective under neutral conditions, which are more practical for electrolyzer operations. This study introduces a dinuclear copper catalyst, [Cu 2 (μ-OH) (μ-NO 3 ) (L) 2 ], where L is the Schiff base ligand derived from salicylaldehyde and histidine amino acid (Complex 1 ), for HER in both aqueous and non-aqueous environments. In non-aqueous conditions, with acetic acid as a proton source, the catalyst exhibits outstanding HER performance, achieving an overpotential of 170 mV. At an acid concentration of 0.086 M, it achieves a catalytic current of ∼17.5 mA cm −2 at an overpotential of 0.52 V. Remarkably, in fully aqueous neutral conditions, Complex 1 reaches a significant current density with an i c /i p ratio of ∼82 at a scan rate of 1.0 V s −1 , and an apparent rate constant ( k obs ) for electrocatalytic HER of ∼13,200 s −1 . The catalyst also shows excellent activity in 0.1 M H 3 PO 4 , with low overpotentials of ∼150 mV at 2.0 mA cm⁻ 2 and 270 mV at 10.0 mA cm⁻ 2 . In controlled potential electrolysis (CPE), the catalyst maintains stable catalytic currents of 25 mA cm⁻ 2 and 75 mA cm⁻ 2 for 18–28 h at a potential of 0.590 V vs. RHE in neutral and acidic conditions, respectively, without noticeable decrease in catalytic current. Detailed characterizations confirm the molecular integrity of the catalyst and its predominantly homogeneous catalytic mechanism.
研究领域:
化学
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材料科学
·
能源科学
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高影响因子
ANALYTICAL SCIENCES
·
2024
·
IF 1.8
Liu Zhili,Yan Yumei,Li Jing,Zhou Wenfeng,Gao Haixiang,Lu Runhua
Heavy metal pollution has always been a great threat to human health and safety. Compared with other heavy metals, although zirconium ion (Zr(IV)) is equally harmful, due to the lack of research on Zr(IV) in the biological systems and environment, its detection does not seem to have received the attention it deserves. Herein, a rapid visual dual-mode detection (colorimetric and chrominance method) of Zr(IV) based on l -histidine functionalized gold nanoparticles (HIS-AuNPs) has been reported. AuNPs and HIS-AuNPs before and after adding Zr(IV) were characterized by UV–Vis, TEM, DLS, Zeta potential, EDS and FT-IR, etc. These results showed that l -histidine was successfully modified on the surface of AuNPs by forming a stable Au–N bond, and its modification had little effect on the dispersion degree of AuNPs. After the addition of Zr(IV), interaction of this metal ion with the imidazolyl group on l -histidine can obviously cause the aggregation of HIS-AuNPs within 12 min, and the dispersion state and particle size of HIS-AuNPs can be significantly changed. These two detection modes were established by means of absorbance and color change of solution, and being used in addition and recovery experiments of Zr(IV) in natural water. Under the optimal conditions, these two modes exhibited good linearity within 15–70 and 20–100 μmol L −1 , and limit of detection of 2.62 and 6.25 μmol L −1 . The proposed method was highly sensitive and selective, which provided a new convenient way to realize the detection of Zr(IV). Graphical abstract
研究领域:
环境科学
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分析化学
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纳米技术
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PubMed
高影响因子
TALANTA
·
2024
·
IF 5.6
Qing Chen,Lijie Zhang,Yang Zhang,Jiajun Shen,Dandan Zhang,Mengmeng Wang
Depletion and separation of histidine-rich proteins from complicated biosamples are crucial for various downstream applications in proteome research and clinical diagnosis. Herein, porous polymer microspheres coated with polyacrylic acid (SPSDVB-PAA) were fabricated through double emulsion interfacial polymerization technique and followed by immobilization of Cu 2+ ions on the surface of SPSDVB-PAA. The as-prepared SPSDVB-PAA-Cu with uniform size and nanoscale pore structure enabled coordination interaction of Cu 2+ with histidine residues in his-rich proteins, resulting in high-performance adsorption. As metal affinity adsorbent, the SPSDVB-PAA-Cu exhibited favorable selectivity for adsorbing hemoglobin (Hb) and human serum albumin (HSA) with the maximum adsorption capacities of 152.2 and 100.7 mg g −1 . Furthermore, the polymer microspheres were used to isolate histidine-rich proteins from human whole blood and plasma, underscoring their effectiveness. The liquid chromatography tandem mass spectrometry (LC-MS/MS) results indicated that the content of 14 most abundant proteins in human plasma was depleted from 81.6 % to 30.7 % and low-abundance proteins were enriched from 18.4 % to 69.3 % after treatment with SPSDVB-PAA-Cu, illustrating potential application of SPSDVB-PAA-Cu in proteomic research.
研究领域:
蛋白质组学
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生物技术
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分析化学
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临床诊断
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PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Shen Zhang,Xiao Wang,Yutong Wang,Jiaxu Dong,Yixuan Zhang,Manlin Zhao
Vitamin B12 is a drug that can participate in the production of bone marrow red blood cells, prevention of pernicious anemia and damage to brain nerves. It is needful to prepare new vitamin B12 determination strategies with excellent sensitivity, selectivity and uniqueness. Herein, a novel and facile chemical reduction method was successfully carried out (histidine and ascorbic acid were applied as protectant and reductant) for preparing histidine-functionalized silver nanoclusters (Ag NCs@his) with blue fluorescence. Some technologies were adopted to clarify the structure, optical characteristics of this stable Ag NCs@his and fluorescence quenching mechanism. Based on inner filter effect, a sensitive and selective platform for vitamin B12 detection was established. Under optimum pH value, reaction time and temperature, the as-developed platform could be utilized to the trace measurement of vitamin B12 with linear range of 0.5–200 μM and detection limit of 0.038 μM. Moreover, this platform was successfully used to measure vitamin B12 in real samples and temperature.
研究领域:
生物化学
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分析化学
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医学检测
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高影响因子
MOLECULES
·
2024
·Vol. 29
·期 12
·2906
·
IF 4.2
Hailin Wen,Miao Li,Chao-Yang Zhao,Tao Xu,Shuang Fu,Huimin Sui,Cuiyan Han
A highly sensitive, selective and recyclable histidine detection method based on magnetic Fe3O4@mTiO2(M-TiO2) nanocomposites with SERRS was developed. Mesoporous M-TiO2nanoparticles were functionalized with 4-aminothiophenol and then coupled with histidine through an azo coupling reaction in 5 min, producing the corresponding azo compound. The strong and specific SERRS response of the azo product allowed for ultrasensitive and selective detection for histidine with an M-TiO2device loaded with Ag NPs due to the molecular resonance effect and plasmonic effect of Ag NPs under a 532 nm excitation laser. The sensitivity was further enhanced with the magnetic enrichment of M-TiO2. The limit of detection (LOD) was as low as 8.00 × 10−12mol/L. The M-TiO2demonstrated applicability towards histidine determination in human urine without any sample pretreatment. Additionally, the M-TiO2device can be recycled for 3 cycles with the photodegradation of the azo product under UV irradiation due to TiO2-assisted and plasmon-enhanced photocatalysis. In summary, a multifunctional and recyclable M-TiO2device was synthesized based on azo coupling and SERRS spectroscopy for ultra-sensitive and specific histidine sensing. In addition, the proposed system demonstrated the potential for the multiplex determination of toxic compounds in the fields of food safety, industrial production and environmental protection, which benefit from the fingerprint property and universality of SERRS.
研究领域:
生物化学
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分析化学
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材料科学
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PubMed
高影响因子
RSC Advances
·
2015
·Vol. 5
·期 75
·61449-61454
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IF 3.84
Yanjia Guo,Xijuan Zhao,Tengfei Long,Min Lin,Zhongde Liu,Chengzhi Huang
Despite a significant surge in the number of investigations into chirality at the nanoscale, there is currently only limited knowledge about the chirality origin and potential applications. Here, we have succeeded in preparing a pair of gold nanocluster enantiomers, in which the histidine (His) enantiomers serve as both the reducing agent and the stabilizing ligand. Origins of the optical activity are discussed from the viewpoint of the intrinsically chiral core model and the dissymmetric field effect. The former is proposed to be responsible for the chirality origin of the as-prepared chiral gold nanoclusters (AuNCs), and both of them act concurrently for the chiroptical response observed in the ligand exchange reactions, which are performed on the His enantiomer-based clusters with enantiopure thiol Pen and Cys ligands, respectively. In addition, the clusters display potential toward biological labelling due to the high photoluminescence (PL) quantum yield and remarkable cellular uptake, in spite of the fact that no chirality-dependent effects in autophagy and subcellular localization are observed in the following fluorescence imaging.
研究领域:
纳米生物学
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材料科学
·
分析化学
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高影响因子
Frontiers in Bioengineering and Biotechnology
·
2021
·
IF 5.89
E Yang,Meng Jun,Cai Heqing,Li Caibin,Liu Sainan,Sun Luming,Liu Yanxiang
The organic compounds from biochar play a role of hormone analogs, stimulating the expression of metabolites by controlling related gene and protein. In this experiment, we reported the L-histidine biosysthesis was promoted by biochar treatment in E. coli unlike genetic engineering of the traditional method. The related results indicated the most optimal concentration was found to be 3%, and 7% is the lethal dose. E. coli was inhibited in the high-concentration treatment. On the other hand, docking technology was usually used as drug screening, basing on Lock-and-key model of protein in order to better understand mechanisms. So the organic compounds of biochar from GC-MS analysis that acted as ligands were connected to HisG protein controlling L-histidine biosysthesis in E. coli. The result showed that the three organic molecules interacted with HisG protein by hydrogen bond. So we considered that these three compounds play regulatory roles in L-histidine biosysthesis, and the hisG gene expression fully supports this conclusion.
研究领域:
微生物学
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生物化学
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分子生物学
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生物技术
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PubMed
高影响因子
ANALYST
·
2018
·Vol. 143
·期 18
·4442-4447
·
IF 3.864
Pengjuan Ni,Dafeng Jiang,Chuanxia Chen,Yuanyuan Jiang,Yizhong Lu,Zhenlu Zhao
In this work, we propose a fluorescence method for the simultaneous detection of glutathione (GSH) and histidine (His) based on the Cu(II)-thiamine (Cu(II)-TH) system. It is well established that non-fluorescent thiamine (TH) can be oxidized by Cu(II) to generate fluorescent thiochrome (TC) under alkaline conditions. The introduction of GSH and His can inhibit the oxidation of TH by Cu(II) due to the strong affinity between Cu(II) and GSH or His. With this strategy, the detection limit for GSH and His is 10.5 nM and 26.4 nM, respectively. The developed method shows several obvious advantages: (1) simplicity in design and operation without the utilization of fluorescent nanomaterials or probes; (2) time-saving detection process since all the detection process is completed within 15 min; (3) cost-effectiveness by using TH as the fluorescent substrate; and (4) higher sensitivity and good selectivity. Therefore, it shows great potential in biosensing fields.
研究领域:
分析化学
·
生物传感
·
医学检测
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PubMed
高影响因子
Journal of Materials Chemistry B
·
2015
·Vol. 3
·期 10
·2185-2191
·
IF 4.726
Jiangnan Zheng,Zian Lin,Guo Lin,Huanghao Yang,Lan Zhang
This work reports a novel metal–organic framework (MOF)-based metal affinity platform for the rapid and highly specific separation of histidine-rich proteins using zeolitic imidazolate framework-8 coated magnetic nanocomposites (denoted as Fe3O4@ZIF-8). The coating of the ZIF-8 layer on the Fe3O4 core was performed in aqueous solution at room temperature and merely took 10 minutes. The monodisperse Fe3O4@ZIF-8 has an average diameter of 190 nm, displays superparamagnetism with a saturation magnetization value of 47.9 emu g−1, and possesses a large external surface area of 131.0 m2 g−1. Due to the high density of low-coordinated Zn atoms on the surface of ZIF-8, Fe3O4@ZIF-8 exhibited a large adsorption capacity for model histidine-rich proteins (>6000 mg g−1 for bovine hemoglobin) and relatively low adsorption capacities for other proteins which contain fewer surface-exposed histidine residues. Moreover, Fe3O4@ZIF-8 showed excellent recyclability (more than 10 times) with high recovery (88.4%). In addition, Fe3O4@ZIF-8 can be used to selectively separate hemoglobin from a protein mixture and human blood samples. The good results demonstrate the potential of Fe3O4@ZIF-8 in the separation of histidine-rich proteins.
研究领域:
生物化学
·
材料科学
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PubMed
高影响因子
危险品化学品经营许可证（带存储）