Journal of the Taiwan Institute of Chemical Engineers
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2022
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IF 5.477
Tianhao Han,Weiwei Chen,Yawen Cai,Zhimin Lv,Yifeng Zhang,Xiaoli Tan
Background Due to the randomly distributed nature of uranium contamination, remediation strategies often concentrate on in-situ immobilization. Limestone can limit the migration of uranium through its spontaneous replacement reaction with phosphate, and achieve uranium immobilization. Methods In this study, the immobilization of uranium in the deposition process of hydroxyapatite was investigated. The effect of carbonate concentration on the reaction process was studied by adding Na 2 CO 3 . Significant findings The deposition process is accompanied by the immobilization of uranium by co-precipitation. The immobilization (92.6%) of uranium (0.52 mmol/L) is due to the formation of calcium uranyl phosphate, increasing carbonate concentration has a significant inhibitory effect on uranium immobilization, and the 50 Mm carbonate can reduce the uranium immobilization efficiency by nearly 20%. XRD, FTIR, Raman, XPS, EA and fluorescence spectroscopy proved that co-precipitation, complexation, and encapsulation were the main mechanisms of uranium immobilization. Through determining the ratios of type-A and type-B substitutions, it reveals the immobilization of uranium weakens due to the competition of carbonate and phosphate. Our study provides the deposition of uranium in the geosphere should be taken into account for remediation and radioactive pollution disposal.
研究领域:
环境科学
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放射性污染治理
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DOI
高影响因子
Biocatalysis and Agricultural Biotechnology
·
2021
·
IF 0
Xijie Li,Juzheng Wang,Diyi Feng,Han Huang,Yuefeng Deng,Chengli Yang,Jie Ouyang,Dali Li,Ruofu Shi
The search for seeking novel immobilized enzyme carriers is a hot topic in the field of enzyme engineering . As a kind of common office consumable, magnetic printing toner has been terribly wasted, leading to potential harm to the environment. We try to use printing toner as carrier for enzyme immobilization to realize waste reuse. In this paper, the laccase extracted from Agaricus bisporus was successfully immobilized on chitosan coated magnetic printing toner by glutaraldehyde linking. The use of printing toner is proved to be feasible. The results show that the optimal temperature of immobilized laccase is 40 °C, and the optimal pH is 3.0. Compared with free laccase, the optimal temperature and optimal pH have been obviously changed after immobilization. And the pH tolerance has been significantly improved, which can remain 53% activity after 5 cycles. Because of the use of magnetic printing toner, it is more convenient to recycle the immobilized enzyme . In this study, printing toner was used as carrier for the immobilization of enzyme and the discarded printing toner recycling was realized.
研究领域:
酶工程
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环境科学
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生物化学
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DOI
CERAMICS INTERNATIONAL
·
2022
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IF 5.532
Guilin Wei,Xiaoyan Shu,Mingfen Wen,Yuexiang Lu,Tao Duan,Shunzhang Chen,Hexi Tang,Jing Chen,Faqin Dong,Yi Xie,Yang He,Xirui Lu
Nuclear waste generated from the nuclear industry should be safely treated because of its harmful effects on the environment and humans. In this study, the mechanism of the as-prepared Gd 2 Zr 2 O 7 for nuclear waste immobilization was investigated as an example in the simulation of waste immobilization under spark plasma sintering (SPS) conditions. The solid solubility limit of the waste in the sintered matrices was 45 mol% at 1725 °C and 1800 °C. The phase transformation from fluorite to pyrochlore could be detected owing to the increase in the ionic radius ratio when the waste was immobilized in the matrix structure. A high sintering temperature promotes transformation by increasing the orderliness of the matrix structure and destroying the crystalline structures to form an amorphous structure, results in a decrease in structural orderliness. Moreover, the leaching rates of Nd, at approximately 3.5 × 10 −8 g·m −2 ·d −1 at 42 d, demonstrated high chemical stability in the matrices doping with 45 mol% simulated nuclear waste. A mechanism is proposed in this study for treating radioactive waste using Gd 2 Zr 2 O 7 ceramic under SPS conditions.
研究领域:
材料科学
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环境科学
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核能工程
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DOI
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
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2024
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IF 8.2
Dengcai Zhang,Junhao Lin,Juan Luo,Shichang Sun,Xi Zhang,Rui Ma,Juan Peng,Fei Ji,Shuaifei Zheng,Zhen Tian,Ning Ma
Biochar with adjustable redox activity is an effective strategy for immobilization of excess arsenic (As(III)) contaminated soil. However, biochar exhibits limitations in terms of electron transfer efficiency and immobilization efficiency due to insufficient activation energy. In this study, As(III) in the soil was rapidly immobilized by adding magnetic biochar (Fe-BC) and introducing microwave irradiation energy to enhance electron transport efficiency. The results showed that the pore structure and iron species (ZVI, Fe 3 O 4 ) loaded onto the biochar could be modulated by controlling the temperature and time of microwave pyrolysis, which enhanced the microwave absorption capacity and the immobilization performance of As. After adding Fe-BC (10 wt%) and treating with microwave irradiation for 3 h, the content of As(III) in the soil was reduced to 54.56 %. Compared with the conventional heating treatment, the percentage of stabilized As (residual form) increased by 11.21 %. The localized hot spots formed through the absorption of microwave energy by biochar promote the formation of arsenic-containing mineral crystals (FeAsO 4 and Fe 3 AsO 7 ), thus enhancing the immobilization efficiency. In addition, microwave-induced electron transfer facilitated the oxidation of As(III) to As(V) by surface quinone and carbonyl groups on the Fe-BC. Density functional theory calculation further proved that the surface groups of the Fe-BC had a stronger electron-withdrawing ability under microwave irradiation, thereby promoting the adsorption and immobilization of As(III). This work provided a new perspective on the technology of rapid remediation of heavy metals contaminated soil using biochar.
研究领域:
环境科学
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土壤修复
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化学工程
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材料科学
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DOI
PubMed
高影响因子
Materials
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2020
·Vol. 14
·期 1
·130
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IF 3.057
Yanshuai Ding,Hao Zhang,Xuelian Wang,Hangtian Zu,Cang Wang,Dongxue Dong,Mingsheng Lyu,Shujun Wang
The immobilization technology provides a potential pathway for enzyme recycling. Here, we evaluated the potential of using dextranase immobilized onto hydroxyapatite nanoparticles as a promising inorganic material. The optimal immobilization temperature, reaction time, and pH were determined to be 25 °C, 120 min, and pH 5, respectively. Dextranase could be loaded at 359.7 U/g. The immobilized dextranase was characterized by field emission gun-scanning electron microscope (FEG-SEM), X-ray diffraction (XRD), and Fourier-transformed infrared spectroscopy (FT-IR). The hydrolysis capacity of the immobilized enzyme was maintained at 71% at the 30th time of use. According to the constant temperature acceleration experiment, it was estimated that the immobilized dextranase could be stored for 99 days at 20 °C, indicating that the immobilized enzyme had good storage properties. Sodium chloride and sodium acetic did not desorb the immobilized dextranase. In contrast, dextranase was desorbed by sodium fluoride and sodium citrate. The hydrolysates were 79% oligosaccharides. The immobilized dextranase could significantly and thoroughly remove the dental plaque biofilm. Thus, immobilized dextranase has broad potential application in diverse fields in the future.
研究领域:
生物化学
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材料科学
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医学
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DOI
高影响因子
APPLIED CLAY SCIENCE
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2020
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IF 4.605
Xiaohai Liu,Baoguo Ma,Hongbo Tan,Li Gao,Pian Chen
Metakaolin (MK) is a widely used admixture in chloride immobilization which is conducive to the durability of reinforced concrete. In this study, triisopropanolamine (TIPA) was added into cement-MK system to improve the chloride immobilization by expediting the pozzolanic reaction of MK. The effect of TIPA on hydration of binder composed of 90% Portland cement and 10% MK was investigated, and the hydration behavior was characterized by thermogravimetric analysis , X-ray diffraction, nuclear magnetic resonance spectroscopy , and mercury intrusion porosimetry , based on the evaluation of pozzolanic reaction of MK by ion dissolution. Results indicated that TIPA promoted the hydration of both MK and cement, and chloride immobilization was also improved. The mechanism for the improvement was involved in three aspects: a) TIPA facilitated the dissolution of aluminum from MK to obtain more chloroaluminate salts, strengthening chemical binding; b) TIPA accelerated hydration of cement and MK for more C-S-H gel, conducive to the physical adsorption; c) TIPA increased the amount of hydrates to refine the pore structure , hindering the migration of chloride ion. This investigation provided one new method for improving the pozzolanic reaction of MK to enhance chloride immobilization of cement-MK system.
研究领域:
建筑材料学
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化学工程
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DOI
高影响因子
JOURNAL OF MATERIALS SCIENCE
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2026
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IF 4.4
Liu Yuchong,Zhao Yu,Yang Wantai,Wang Guan,Zhao Changwen,Liu Y.,Zhao Y.,Yang W.,Wang G.,Zhao C.
Biomass resources represent a sustainable alternative to fossil-based feedstocks for energy and value-added chemical production, and their utilization hinges largely on cellulase-catalyzed glucose conversion. Various immobilization strategies have been developed with the aim of cellulase recyclability. However, these strategies suffer from inadequate interactions between the substrate and immobilized cellulases, as well as poor recyclability. To overcome these drawbacks, we developed a hierarchical polymer brush architecture on polypropylene fibers through sequential photoinduced grafting and surface-initiated atomic transfer radical polymerization. This platform features hydrophilic polymer segments followed by reactive epoxy-functionalized brushes for covalent cellulase immobilization, enhancing substrate-enzyme interactions and cellulase stability against environmental alternations (especially harsh conditions). Importantly, the immobilized biocatalyst maintained 60% initial activity through six cycles in sodium carboxymethyl cellulose hydrolysis. This fiber-grafted block copolymer platform demonstrates an efficient strategy for cellulase immobilization, which opens up a new avenue for high-performance enzyme immobilization in industrial biocatalysis. Graphical abstract
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DOI
高影响因子
Journal of Future Foods
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2026
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IF 10.6
Jinchao Hou,Haohua Hu,Shuaiqi Yan,Le Zhong,Geling Kuang,Yuxiao Feng,Hongwu Wang,Jiandong Cui
A green DES/TX-100/water multicomponent interfacial system was constructed for lipase immobilization. • Hydrophobic hollow bowl-shaped mesoporous carbon (H-HBMC) served as an interfacial activation support. • AOL@H-HBMC-L/DTW achieved 108.43% activity recovery and 1.75-fold higher k cat / K m. • The interface induced lid opening, hydrophobic surface exposure, catalytic triad rearrangement, and enhanced organic solvent/thermal stability. Lipase has a wide range of applications in the food industry, such as baked food, dairy products, and oil modification. However, natural free lipase exhibits the poor stability and low activityin in dustrial applications. Inspired by the interfacial catalysis of lipase, herein, lauric anhydride-modified hydrophobic SiO 2 -derived hollow bowl-shaped mesoporous carbon particles (HBMC) were prepared and utilized to immobilize lipase from Aspergillus oryzae (AOL) in a multicomponent interfacial system composed of Deep eutectic solvents/Triton X-100/water (DTW). The resultant immobilized lipase (AOL@H-HBMC-L/DTW) exhibited higher activity recovery (108.43%) than free lipase. Meanwhile, the catalytic efficiency ( k cat / K m ) of immobilized lipase was 1.75-fold that of free lipase. In addition, thermal stability and organic solvent tolerance were also enhanced. After heating at 55 °C for 25 min, the immobilized lipase could retain 41.87% of its initial activity, whereas free lipase only maintained 29.63% of its initial activity. After dimethylformamide (DMF) treatment for 1 h, the free lipase retained only 50.12% of its activity, whereas the immobilized lipase retained 90.14%. Furthermore, it was demonstrated that hydrophobic H-HBMC-L and the multicomponent interfacial system could facilitate lipase activation and improve its activity. Therefore, we believe that the strategy proposed here has potential in preparing lipase biocatalysts with high activity and stability. Download: Download high-res image (203KB) Download: Download full-size image
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DOI
高影响因子
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
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2015
·Vol. 42
·期 2
·189-196
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IF 2.439
Zhu Deyi,Li Xia,Liao Xuepin,Shi Bi
Collagen fiber (CF), an abundant natural biopolymer, features many favorable properties that make it a potential carrier for cell immobilization. In the present investigation, CF was grafted with polyethyleneimine (PEI) using glutaraldehyde (GA) as the cross-linking agent, resulting in the formation of a novel CF based carrier (CF-PEI). The properties of CF-PEI as a carrier were evaluated by the immobilization of Microbacterium arborescens (CICC 20196), which has glucose isomerase (EC 5.3.1.5) activity. It was found that M. arborescens cells immobilized on CF-PEI exhibited higher glucose isomerization than those using activated carbon or anion exchange resin as the carriers. The Michaelis constant (K m) of the isomerization reaction for the CF-PEI-immobilized M. arborescens cells was 0.528 mol/L, which was slightly higher than that of free cells (0.473 mol/L). In addition, the apparent activation energies (E a) of free and immobilized cells on CF-PEI were almost the same at 60 kJ/mol. In an isomerization reaction of glucose to fructose in a fixed-bed reactor, CF-PEI-immobilized M. arborescens cells showed appreciable activity and operational stability. The corresponding isomerization ratio was as high as 41 % for 20 days, and the half-life was about 40 days.
研究领域:
生物技术
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生物工程
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酶技术
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DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2024
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IF 13.3
Qiushi Jiang,Yanjing Li,Minmin Wang,Wen Cao,Xueying Yang,Sihu Zhang,Liejin Guo
This paper presents a promising heterogeneous biocatalyst-immobilized cellulase system, which can facilitate the targeted catalytic conversion of biomass to reducing sugars. Magnetic Zeolite Imidazole Framework-8/chitosan (Fe 3 O 4 /ZIF-8/CS, FZC) hybrid honeycomb microspheres were synthesized to immobilize cellulase using an in-situ growth strategy. The enhancement of immobilization efficiency induced by each constituent of the composite carrier was explored. This exploration was grounded in varying combinations of components within the honeycomb composite spheres, revealing the indispensability of ZIF-8. Through in-situ assembly, the composite material presents a microsphere with an internal honeycomb structure and multilevel pores. Effective immobilization, temperature-responsive conformational change, and cyclic stability were achieved due to the scaling effect of the channel, extensive specific surface area, and abundant amino group interactions enable immobilized enzymes to maintain excellent performance under challenging operating conditions, demonstrating an enhanced structure-performance relationship. With the obtained immobilized cellulase, we achieved a high load (461.67 mg/g) of cellulase and a significant reducing sugar yield (328.39 mg/g) from corncob waste. In addition, we investigated the enhancement effect of thermal activation on the immobilized enzyme activity of composite carrier. The results showed that the appropriate heat treatment was conducive to the activation of FZC immobilized enzyme with a reducing sugar yield 1.61 times higher than that of the control. The strategy provided in this study promotes the development of immobilized biocatalysts and provides a useful reference for the further design of biocatalytic systems.
研究领域:
生物化学
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材料科学
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酶工程
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DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2019
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IF 3.858
Jia Liu,Run-Tian Ma,Yan-Ping Shi
With the increasing demand for lipase inhibitors and new drugs used in the clinical treatment of obesity, it is of great significance to screen lipase inhibitors from traditional Chinese medicines (TCMs) via capillary electrophoresis. In this work, Fe 3 O 4 @TiO 2 nanoparticles was fabricated by solvothermal method and employed as an improved magnetic support to immobilize lipase through electrostatic interaction. By the method of transmission electron microscopy, fourier transform infrared spectroscopy and X-ray diffraction, the magnetic nanoparticles were characterized. The immobilized enzyme possessed advantages of a wider range for pH and temperature endurance, better storage stability and reusability. The kinetics performances of the immobilized lipase were studied. When p-Nitrophenyl palmitate (pNPP) was used as enzyme substrate, the Michaelis-Menten constant was calculated to be 2.51 mM and its inhibition constant for Orlistat was ascertained to be 13.41 μM. Ultimately, the established method was applied to lipase inhibitors screening from 6 Tibetan medicines with lipase inhibitory activity and Oxytropis falcate Bunge was screened out for its supreme lipase inhibitory activity. 11 compounds in the Oxytropis falcate Bunge were further screened, five compounds exhibited similar inhibitory activity to Orlistat, and one compound (kaempferol) presented better inhibitory activity than Orlistat, which is the most commonly used drugs to treat obesity in clinic. This work not only developed a method for new anti-obesity drugs discovery, but also provided inspiration for exploring new medicinal value of the TCMs.
研究领域:
药学
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生物技术
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中药学
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药物发现
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DOI
PubMed
高影响因子
ChemCatChem
·
2021
·Vol. 13
·期 23
·5047-5055
·
IF 5.686
Cong Gao,Jiaping Wang,Liang Guo,Guipeng Hu,Jia Liu,Wei Song,Liming Liu,Xiulai Chen
Graphical Biocatalysis : In this study, an efficient synthetic microbial consortium for glutaric acid production was developed by dividing the cadaverine pathway into two engineered strains. Moreover, strategies of cofactor regeneration and colloidal chitin based-immobilization were implemented to provide a low-cost approach for glutaric acid biosynthesis under mild reaction conditions. Glutaric acid is an important C5 platform compound for the synthesis of nylon polymers. However, its production titer and cost are limited by the need for expensive cofactors addition as well as the difficulty of reusing microbial catalysts. Herein, to address these problems, two regeneration systems each for NAD(P) + and α-ketoglutaric acid were introduced into a five-enzyme cadaverine pathway for a whole-cell biocatalytic approach. We demonstrated the efficient conversion of lysine to glutaric acid in a synthetic microbial consortium manner, producing 73.2 g/L glutaric acid with a 74 % molar yield in a 5-L bioreactor scale. Moreover, with a colloidal chitin-based immobilization, the engineered microbial consortium exhibited good stability and reusability in a five-cycle bioconversion. This study provides an efficient microbial platform for glutaric acid production by developing a cheaper and universal cell immobilization approach.
研究领域:
生物技术
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生物化工
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代谢工程
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DOI
高影响因子
危险品化学品经营许可证（带存储）