TALANTA
·
2012
·
IF 3.794
Wei Chen,Lei Hong,Ai-Lin Liu,Jian-Qing Liu,Xin-Hua Lin,Xing-Hua Xia
As a peroxidase mimic, cupric oxide nanoparticles were found to enhance the chemiluminescence (CL) of luminol-H 2 O 2 system up to 400 folds. The CL spectra and radical scavengers were conducted to investigate the possible CL enhancement mechanism. It was suggested that the enhanced CL could be attributed to the peroxidase-like activity of CuO nanoparticles, which effectively catalyzed the decomposition of hydrogen peroxide into hydroxyl radicals . The effects of the reactant concentrations and some organic compounds were also investigated. The proposed method could be used as a sensitive detection tool for hydrogen peroxide and glucose.
研究领域:
生物化学
·
分析化学
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DOI
PubMed
高影响因子
Analytical Methods
·
2017
·Vol. 9
·期 6
·974-979
·
IF 1.9
Jian Xin Xie,Wen Jing Chen,Xian Xue Wu,Yun Ying Wu,Hong Lin
It was found that Co–Fe layered double hydroxides (Co–Fe LDHs), other than intercalated LDHs, could catalyze the luminol–H2O2 chemiluminescence (CL) reaction and generate a strong CL signal. The as-prepared Co–Fe LDHs were characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Based on the CL spectra, UV-visible absorption spectra and free radical scavenging assay, a possible CL mechanism was proposed. Under optimum conditions, a good linear relationship between the CL intensity and H2O2 concentration in the range of 1.0 × 10−8 to 3.0 × 10−6 mol L−1, with the limit of detection of 5.0 × 10−9 mol L−1, was obtained. Based on the glucose conversion into H2O2 by glucose oxidase, the sensitive detection of glucose could be realized with the limit of detection of 2.0 × 10−8 mol L−1. This proposed method has been applied to the determination of H2O2 and glucose in real samples with satisfactory results.
研究领域:
分析化学
·
生物医学工程
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DOI
高影响因子
ACS Applied Materials & Interfaces
·
2019
·
IF 8.456
Yue Song,Wei Zhang,Shuijian He,Lei Shang,Rongna Ma,Liping Jia,Huaisheng Wang
In the field of clinical diagnosis, it is important to construct a potential-resolved multiplex electrochemiluminescence (ECL) biosensor for decreasing the false-positive rate and improving the diagnostic accuracy. However, the shortage of low-potential cathodic luminophores between −1 and 0 V (vs Ag/AgCl) severely limited the development of the biosensor. Herein, we synthesized a novel luminophore N,N-bis-(3-dimethyl aminopropyl)-3,4,9,10-perylene tetracarboxylic acid diimide (PDI), which gave dual emissions at −0.25/–0.26 V with K2S2O8 as a co-reactant in aqueous solution. The ECL was assigned to excited J-type PDI dimers. Then, PDI and luminol were used as luminophores to respectively combine with graphite oxide and gold nanoparticles and form potential-resolved ECL nanoprobes. Also, this potential-resolved ECL nanoprobes were respectively functionalized by secondary antibodies (Ab2) to construct a low-potential sandwiched ECL immunosensor for tumor markers carcinoembryonic antigen (CEA) and α-fetoprotein (AFP) simultaneous determination during linear scanning potential range from −0.6 to 0.6 V. The prepared multiplex immunosensor exhibited sensitive ECL response for CEA at −0.6 V due to PDI and that for AFP at 0.6 V due to luminol, and both linear semilogarithmical ranges were from 0.1 pg to 1 ng mL–1. In addition, PDI with dual ECL peaks showed enticing prospect of built-in self-calibration for a precise quantitative and bioimaging analysis.
研究领域:
生物医学工程
·
分析化学
·
临床诊断
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DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2019
·Vol. 411
·期 20
·5175-5186
·
IF 3.286
Wei Lu,Zhang Yanhui,Eziz Nurguzal,Yang Yaru,Li Guixin,Guan Ming
An ultrasensitive electrochemiluminescence (ECL) immunosensor for alpha-fetoprotein (AFP) detection based on a poly(aniline-luminol)/graphene oxide nanocomposite was developed. The nanocomposite, which was prepared using a fast and simple chemical oxidation strategy for the first time, showed excellent ECL performance. This outstanding ECL performance is due to the formation of poly(aniline-luminol) on the graphene oxide (GO) surface and the excellent electron-transfer properties of GO. Moreover, the poly(aniline-luminol)/graphene oxide nanocomposite has abundant amino groups at its surface, making it a good platform for biomacromolecule labeling. Using the nanocomposite, a novel ECL immunosensor for the determination of AFP was successfully developed. Anti-AFP was immobilized on the surface of a poly(aniline-luminol)/graphene oxide nanocomposite-modified electrode using a glutaraldehyde crosslinking method to form the ECL immunosensor. The AFP was then captured at the modified electrode surface through an antigen–antibody immunoreaction. When the AFP was captured by its antibody, the ECL intensity decreased. This ECL immunosensor for the detection of AFP exhibited a linear range of 1.7 × 10 −12 to 1.7 × 10 −8 mg mL −1 and a detection limit of 5 × 10 −13 mg mL −1 , indicating high sensitivity and linearity across a wide concentration range. Furthermore, the immunosensor was successfully applied to determine AFP in a real-world human serum sample.
A new poly(aniline-luminol)/graphene oxide nanocomposite was prepared using a fast and simple strategy for the first time, and an ultrasensitive electrochemiluminescence (ECL) immunosensor for alpha-fetoprotein (AFP) detection based on this nanocomposite was developed
研究领域:
分析化学
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生物传感器
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纳米技术
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医学诊断
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DOI
PubMed
高影响因子
ELECTROANALYSIS
·
2022
·Vol. 34
·期 10
·1555-1563
·
IF 3.077
Ruirui Dong,Yanhui Zhang,Jiali Huang,Marhaba Habibul,Guixin Li
A novel electrochemiluminescence biosensor was presented for the assay of hepatitis B virus (HBV) DNA. Coralloid poly(aniline-luminol)-MWCNTs was prepared on an electrode surface, which was followed by the attachment of capture DNA via amide bond formation. The HBV-DNA and ferrocene labelled DNA hybridized with capture DNA to form a sandwich structure on electrode surface. Ferrocene can effectively increase the signal output of the ECL sensor due to the catalysis of ferrocene on electrochemiluminescence of luminol. The biosensor rendered a linear dependence over the range from 1.0×10 −16 to 1.0×10 −7 M with a low detection limit of 2.4×10 −17 M.
研究领域:
分析化学
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生物传感器
·
病毒学
·
电化学
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DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2015
·Vol. 40
·期 1
·458-463
·
IF 3.086
Huimin Duan,Leilei Li,Xiaojiao Wang,Yanhui Wang,Jianbo Li,Chuannan Luo
A trace-level chemiluminescent (CL) sensor for the determination of phenacetin, utilising CdTe quantum dots@luminol (QDs@luminol) for signal amplification, based on a chitosan/magnetic graphene oxide-molecularly imprinted polymer (CsMG-MIP) as a biological recognition material was fabricated. CdTe QDs@luminol, which was used in the preparation process for the sensor, could amplify the signal of the CL sensor through chemiluminescence resonance energy transfer (CRET) while reducing the consumption of luminol. The CsMG-MIP, taking full advantage of the abundant hydroxyl and amino groups in chitosan which provide a lot of sites for the formation of hydrogen bonds in SMIP, using graphene oxide to improve the adsorption capacity and Fe3O4 nanoparticles to make the preparation of the recognition unit simple and easy, was introduced into the CL sensor. Under the optimized conditions of the CL sensor, phenacetin could be assayed in the range of 3.0 × 10−9–3.0 × 10−7 mol L−1 with a detection limit of 8.2 × 10−10 mol L−1 (3δ). With the advantages of amplifying the CL signal through CRET and reducing the consumption of luminol simultaneously, the sensor was successfully applied to the determination of trace-level phenacetin in real samples with a high selectivity and with the reagent economized.
研究领域:
分析化学
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生物传感
·
药物分析
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DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2016
·
IF 4.758
Qi Li,Jiahua Wang,Yi He
A selective chemiluminescent sensor was developed for the detection of mercury(II) ions (Hg 2+ ) in environmental water and soil supernatant samples using non-aggregated luminol-capped gold nanoparticles (AuNPs). Luminol-capped AuNPs can react with silver nitrate (AgNO 3 ) under alkaline environment , generating a strong chemiluminescence (CL) emission because AuNPs had excellent catalytic activity for luminol-AgNO 3 CL system. However, when Hg 2+ was added to the solution of luminol-capped AuNPs, it would be adsorbed on the surface of luminol-capped AuNPs according to the inherent affinity of AuNPs for Hg 2+ . The presence of Hg 2+ significantly inhibited the catalytic activity of AuNPs, leading to CL quenching. With the increasing of Hg 2+ concentration, the CL intensity gradually decreased. Under the optimal detection conditions, the developed CL sensor showed a good linear relationship in range of 10–600 nM with a limit of detection of 1 nM at a signal-to-noise ratio of three which was below the maximum allowable concentration for Hg 2+ in drinking water (10 nM) defined by United States Environmental Protection Agency. Also, the developed CL sensor exhibited high selectivity toward Hg 2+ over other environmentally relevant metal ions at high concentrations (500 μM).
研究领域:
分析化学
·
环境科学
·
纳米技术
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产品关联
DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2025
·
IF 3.8
Zhang Wei,Chen Yequan,Ji Kaixiang,Dong Zhiyong,Abdussalam Abubakar,Zholudov Yuriy T.,Gao Wenyue,Tian Yu,Xu Guobao
Vanadyl acetylacetonate, VO(acac) 2 , is a well-known environmentally friendly catalyst for aerobic oxidations. It has been exploited as an efficient co-reactant of luminol chemiluminescence (CL) in aqueous media for the first time. The luminol-VO(acac) 2 system generates an intense CL and ascorbic acid can quench its CL remarkably. Based on the strong quenching effect of ascorbic acid, the luminol-VO(acac) 2 CL was used in the quantification of clinically important ascorbic acid and alkaline phosphatase (ALP) by enzymatic conversion of L-ascorbic acid 2-phosphate into ascorbic acid. The linear ranges for detecting ascorbic acid and alkaline phosphatase are 1 to 100 μM and 0.005 to 200 U/L, respectively. The limits of detection of ascorbic acid and alkaline phosphatase are 0.5 μM and 0.005 U/L, respectively. The proposed CL method has shown high selectivity for the ALP among the tested biomolecules and metal ions. It also shows good recoveries in human serum samples. Graphical
研究领域:
分析化学
·
生物医学
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Chao Li,Juan Lu,Yanjia Guo,Yujia Song,Ruidan Li,Hanyue Jiang,Wenzhuo Wang,Pengfei Han,Guangping Ma,Jing Wang,Li Tian
In this paper, a novel dual-potential ratiometric electrochemiluminescence (RECL) sensor was constructed and applied to the ultrasensitive detection of kanamycin (KAN). The RECL sensor was constructed using porous graphite phase carbon nitride (PCN) and luminol, which serve as the luminescent reagents at the cathode and anode, respectively. PCN not only functioned as the luminescent reagent for the cathode but also served as a carrier for the Pt Au NPs. Simultaneously, Ti 3 CN acted as a carrier for the anode luminescent reagent, luminol, thereby enhancing the ECL signals at anode. Under optimal conditions, the ratio of the anode to cathode ECL signals (I luminol /I PCN ) showing excellent linear relationship with the logarithm of KAN concentration (lgC KAN ) within the range of 5.0 × 10 −14 to 5.0 × 10 −8 mol/L, with a detection limit as low as 1.67 × 10 −14 mol/L, making it suitable for detecting KAN in milk samples.
研究领域:
分析化学
·
食品安全检测
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2014
·
IF 3.84
Zhiyong Guo,Beibei Chen,Zebo Wang,Xiaohua Jiang
An ultrasensitive electrochemiluminescence (ECL) biosensor based on gamma-polyglutamic acid-graphene-luminol (γ-PGA-G-luminol) composite and oligonucleotides for the detection of mercury ion (Hg 2+ ) was developed. γ-PGA-G-luminol composite coated on the glassy carbon electrode could produce a strong and stable ECL signal by luminol, and provide carboxyl groups to cross-link with amino-modified DNA1 enriching in thymine (T) by γ-PGA. Upon addition of Hg 2+ and biotin-modified DNA2 enriching in T, T–Hg 2+ –T interaction occurred and mediated the coordination between DNA1 and DNA2. Afterwards, streptavidin was connected to the end of DNA2 through specific binding of streptavidin to biotin, which obviously reduced the ECL intensity due to the inert protein layer with poor electron transfer ability. Based on the specific T–Hg 2+ –T coordination chemistry and the specific binding of streptavidin-biotin system, ultrahigh sensitivity and selectivity for Hg 2+ detection were obtained. Results revealed that the ECL intensity was logarithmically linear with the concentration of Hg 2+ in a wide range from 0.01 to 100 nmol/L (about 0.002–20 ppb). Moreover, the biosensor also exhibited excellent selectivity for Hg 2+ ions without significant interference from commonly co-existing metal ions in the sample matrix. Excellent sensitivity and selectivity make the developed biosensor a potential and simple tool for the detection of Hg 2+ .
研究领域:
分析化学
·
环境科学
·
生物技术
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DOI
高影响因子
ACS Applied Materials & Interfaces
·
2021
·
IF 9.229
Hui Ouyang,Jiaxin Xian,Shuai Luo,Lvxia Zhang,Wenwen Wang,Zhifeng Fu
A facile, one-step doping protocol was adopted to synthesize Co single atomic site catalysts (SASCs) in UiO-66 metal–organic frameworks. In view of highly uniform active sites of Co–O6 moieties, the SASCs specifically contribute to catalyzing the generation of a large amount of singlet oxygen instead of superoxide or hydroxyl radicals, which endows Co SASCs with a the remarkable enhancement effect (∼3775 times) on luminol chemiluminescent (CL) emission. Interestingly, monolayer titanium carbide MXenes can drastically quench the CL signal of the Co SASC-boosted luminol reaction by ∼94.6% as highly efficient luminescent absorbents. Furthermore, the emitter–quencher pair of Co SASCs and titanium carbide MXenes was successfully adopted to develop an immunoassay method for cardiac troponin I (cTnI) on an immunochromatographic test strip platform. With a sandwich immunoreaction mode, a titanium carbide MXene-labeled cTnI tracer antibody was captured on the test line of a test strip, which significantly inhibited the CL response of the Co SACs-boosted luminol system. The dynamic range for quantitating cTnI is 1.0–100 pg mL–1, with a detection limit of 0.33 pg mL–1 (3σ). The test strip was successfully used to detect cTnI in human serum samples collected from cardiopathy patients. This proof-of-principle work manifests both the CL enhancement of SASCs and the quenching behavior of MXenes, which shows the thrilling prospects of combinational usage of the two functionalized nanomaterials for tracking biological recognition events.
研究领域:
分析化学
·
材料科学
·
生物医学工程
·
纳米技术
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DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2024
·
IF 3.8
Zhang Yahui,Wang Xinyi,Jia Changbo,Dong Yongping
The majority of previously reported cathodic electrochemiluminescence (ECL) systems often required very negative potential to be carried out, which has greatly limited their applications in the sensing field. Screening high-performance cathodic ECL systems with low triggering potential is a promising way to broaden their applications. In this work, rhenium disulfide nanosheets (ReS 2 NS) have been revealed as an efficient co-promoter to realize low-triggering-potential cathodic luminol ECL. One strong cathodic ECL signal appeared at a potential of −0.3 V and one anodic ECL peak was obtained at −0.15 V under the reverse potential scan, which were caused by electrogenerated reactive oxygen species (ROS) from hydrogen peroxide. The generation of strong luminol ECL at low potential was the result of the electrocatalytic effect of ReS 2 NS on the reduction of H 2 O 2 . The scavenging effect of uric acid (UA) on the ROS could significantly inhibit the cathodic ECL. As a result, an ECL sensor was proposed, which showed outstanding performance for the detection of UA in the range of 10 nM to 0.1 mM with a low detection limit of 1.53 nM. Moreover, the ECL sensor was successfully applied in the sensitive detection of UA in real samples. This work provides a new avenue to establish a low-potential cathodic ECL system, which will sufficiently expand the potential application of cathodic ECL in the sensing field.
研究领域:
分析化学
·
生物医学工程
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）