Micromachines
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2023
·Vol. 14
·期 3
·500
·
IF 3.4
Shi Tang,Zhaoqin Wei,Jiani Guo,Xiaoxia Sun,Yu Hu
A novel triazole fluorescent sensor was efficiently synthesized using binaphthol as the starting substrate with 85% total end product yield. This chiral fluorescence sensor was proved to have high specific enantioselectivity for lysine. The fluorescence intensity ofR-1was found to increase linearly when the equivalent amount of L-lysine (0–100 eq.) was gradually increased in the system. The fluorescence intensity of L-lysine toR-1was significantly enhanced, accompanied by the red-shift of emission wavelength (389 nm to 411 nm), which was attributed to the enhanced electron transfer within the molecular structure, resulting in anICTeffect, while the fluorescence response of D-lysine showed a decreasing trend. The enantioselective fluorescence enhancement ratio for the maximum fluorescence intensity was 31.27 [ef = |(IL−I0)/(ID−I0)|, 20 eq. Lys], thus it can be seen that this fluorescent probe can be used to identify and distinguish between different configurations of lysine.Keywords:enantioselective recognition;ICT effect;intermolecular hydrogen bond
研究领域:
化学
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生物化学
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PubMed
高影响因子
Polymer Chemistry
·
2013
·Vol. 4
·期 22
·5597-5602
·
IF 5.231
Zengchao Tang,Xiaoli Liu,Yafei Luan,Wei Liu,Zhaoqiang Wu,Dan Li,Hong Chen
A new strategy to regulate the attachment of plasminogen and its activator, t-PA, to a polyurethane surface is reported. The method is based on the one-step graft copolymerization of a lysine-containing methacrylic monomer and hydroxyethyl methacrylate (HEMA). The copolymer-modified PU surfaces showed high capacity for plasminogen and t-PA binding. The release of pre-adsorbed t-PA from the lysine-containing PU surfaces in contact with plasma was also investigated. It was shown that the interactions of plasminogen and t-PA on this type of surface can be controlled simply by changing the monomer feed ratio, and thus the relative quantities of HEMA and lysine in the grafts. This approach may be useful for the development of fibrinolytic, blood-contacting materials and, more generally, for controlling protein-surface interactions for biocompatibility.
研究领域:
生物材料学
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医学工程
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DOI
高影响因子
JOURNAL OF THE TEXTILE INSTITUTE
·
2025
·
IF 1.5
Yameng Xiao,Hualei Liu,Chen Sun,Xinran Han,Roman A. Surmenev,Xia Yin,Yi-Tao Liu,Jianyong Yu,Bin Ding
Porous carbon materials are widely used in adsorption and catalysis due to their highly adaptable pore structures and high specific surface areas. Nevertheless, porous carbon materials still face the bottleneck problem of mechanical strength and flexibility. Herein, we construct a porous carbon nanofiber membrane composed of beaded fibers and traditional straight fibers, demonstrating exceptional flexibility and efficient formaldehyde adsorption performance. Specifically, we regulate polyacrylonitrile solutions with different concentrations via a dual-nozzle electrospinning technique, followed by using controllable pre-oxidation and carbonization progress, obtaining flexible carbon nanofiber material with a hierarchical micro/mesoporous and beaded network structure. The softness of porous carbon nanofiber membrane is comparable to nonwoven fabrics and significantly lower than tissue paper, and it goes through 1000 buckling cycles at 50% deformation without damage. Furthermore, dynamic impregnation with lysine endows materials with synergistic physical and chemical adsorption, making its formaldehyde adsorption capacity reached 3.44 mg/g under ambient conditions, which shows great performance improvement compared with unimpregnated materials (0.8 mg/g). In this sense, this material overcomes the traditional trade-off between rigidity and adsorption performance in carbon materials, offering a possible solution for indoor formaldehyde purification.
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RSC Advances
·
2020
·Vol. 10
·期 41
·24507-24514
·
IF 3.119
Peihong Li,Xueyan Dai,Lijie Qu,Yanlong Sui,Chunling Zhang
Peptide-containing hydrogels have become a research hotspot due to their unique secondary structure and biocompatibility. Herein, we used amino-terminated F127 as a macroinitiator to initiate the ring-opening polymerization of L-lysine(z)-NCA, and the obtained oligo(lysine)-modified F127 (FL) had degrees of polymerization of lysine of 2, 5, and 8. The results showed that the FL hydrogels had reversible temperature-dependent sol–gel transitions, and the introduction of lysine increased the critical gel temperature. In the dilute solution of FL, the micelle size increased and aggregated as the pH increased; the micelle grew into a rod-like shape under alkaline conditions. Scanning electron micrographs showed that the interior of the FL hydrogel had a more complete porous structure. The FL-2 hydrogel loaded with 5-fluorouracil exhibited an approximately linear release trend within 12 h and has good biocompatibility. Therefore, FL hydrogels have potential applications in the field of biomedicine.
研究领域:
生物材料
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药剂学
·
高分子化学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2012
·
IF 2.453
Dezhong Zhou,Congxin Li,Yuling Hu,Hao Zhou,Jiatong Chen,Zhengpu Zhang,Tianying Guo
Poly( l -lysine) (PLL) has excellent plasmid DNA (pDNA) condensation capacity. However, the relatively high cytotoxicity and low transfection efficiency limit its application as gene delivery vectors. Here, well-defined glycopolymers are synthesized by reversible addition fragmentation transfer polymerization and grafted onto PLL to improve the gene delivery performance. After glycopolymer modification, PLL shows reduced cytotoxicity. By regulating the glycopolymer length and amino group substitution degree, the glycopolymer modified PLL can condense pDNA with proper strength, protect the condensed pDNA from degradation and release them in time. Transfection with NIH3T3 and HepG2 cells shows that the glycopolymer modified PLL has improved transfection efficiencies. The low cytotoxicity, effective pDNA protection and enhanced transfection efficiencies indicate that glycopolymer modification would be an effective strategy to improve the polycation properties for gene delivery.
研究领域:
生物材料学
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分子生物学
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基因治疗
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PubMed
高影响因子
Animals
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2020
·Vol. 10
·期 2
·251
·
IF 2.323
Jie Peng,Mao Xia,Jia Xiong,Chenbin Cui,Ningning Huang,Yuanfei Zhou,Hongkui Wei,Jian Peng
Simple SummaryHydrogen sulfide (H2S) is an important second messenger, which has been implicated in regulating placental angiogenesis. Our findings revealed that gestational dietary methionine could affect maternal and placental H2S concentrations. With the increase of dietary methionine, maternal plasma and placental H2S concentrations changed quadratically, which was consistent with placental vascular density and reproductive performance. The decrease in H2S production caused by an increase in dietary methionine was likely to be the cause for the increase in the rate of low birth weight piglets and needs further study.AbstractThe placenta is a unique bond between the mother and the fetus during pregnancy, and a proper placental angiogenesis is vital for fetal development. H2S is an endogenous stimulator of angiogenesis that is mainly produced by the methionine transsulfurationpathway. The goal of this study was to evaluate the effect of gestational dietary methionine on maternal and placental H2S production in sows. Multiparous sows (Large×White; third parity;n= 65) were randomly allocated into five groups, with feed diets comprisingstandardized ileal digestible methionine/lysine (Met/Lys) ratios of 0.27 (nutrient requirements of swine (NRC); 2012 level), 0.32, 0.37, 0.42, and 0.47, respectively. The litter size and weight at birth were measured and recorded. Maternal blood samples were obtained at embryonic day (E) E40 d, E90 d, and E114 d of gestation. The placental samples were collected at parturition. The results showed that maternal plasma H2S concentration was not affected at E40 d. However, the maternal plasma H2S concentration changed quadratically with the dietary Met/Lys ratio at E90 d (p< 0.01) and E114 d (p= 0.03). The maximum maternal plasma H2S concentration was at the dietary Met/Lys ratio of 0.37. Meanwhile, maternal plasma H2S concentration was positively correlated with piglets born alive (p< 0.01) and litter weight (p< 0.01). Consistent with the maternal plasma, the placental H2S concentration also changed quadratically with the dietary Met/Lys ratio (p= 0.03); the Met/Lys ratio of 0.37 showed the maximum H2S concentration. In conclusion, our findings revealed that the gestational dietary Met/Lys ratio could affect maternal and placental H2S concentrations, which may be an important molecular mechanism affecting placental angiogenesis and piglet development.Keywords:hydrogen sulfide;reproductive performance;pregnant sows;methionine;placental angiogenesis
研究领域:
动物营养学
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生殖生物学
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PubMed
高影响因子
International Journal of Polymeric Materials and Polymeric Biomaterials
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2021
·
IF 2.604
Yan Zhang,Hai-Chao Zhao,Li-Jing Zhu
Mussel-inspired bionics technology has attracted extensive attention for the surface modification of porous materials. The adhesion effect of the mussel mucin comes is mainly from hydroxylation. Hence, polysulfone membrane was modified by co-deposition of catechol with hydroxyl and L-lysine with biocompatibility. Flux of the membrane exceeds 120 L m−2 h−1, the ultra-high rejection rate to bovine serum albumin and lysozyme. Moreover, the modified membranes exhibited enhanced antifouling ability, antibacterial property and adhesion resistance to E. coli with the synergy between catechol and L-lysine. The fabricated membranes can further lay the foundation for the industrial production of deposited membranes.
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高影响因子
ACS Omega
·
2024
·
IF 3.7
Ting Sun,Faxing Zhang,Yuyi Xu,Xiaowei Wang,Jiajia Jia,Lihong Sang,Ji Li,Dongkai Wang,Zhiguo Yu
Nonsteroidal anti-inflammatory drugs (NSAIDs) combined with chemotherapeutic agents for the treatment of colorectal cancer (CRC) are a promising therapeutic strategy. NSAIDs can effectively boost the antitumor efficacy of chemotherapeutic agents by inhibiting the synthesis of COX-2. However, hazardous side effects and barriers to oral drug absorption are the main challenges for combination therapy with chemotherapeutics and NSAIDs. To address these issues, a safe and effective lysine–polydopamine@abemaciclib-flurbiprofen (Flu) codrug nanocrystal (Lys-PDA@AF NCs) was designed. Abemaciclib (Abe), a novel and effective inhibitor of the CDK4/6 enzyme, and Flu were joined to prepare Abemaciclib-Flu codrug (AF) by amide bonds, and then the AF was made into nanocrystals. Lysine-modified polydopamine was selected as a shell to encapsulate nanocrystals to enhance intestinal adhesion and penetration and lengthen the duration time of drugs in vivo. Nuclear magnetic resonance, Fourier transform infrared, Massspectrometry, X-ray photoelectron spectroscopy, Transmission electron microscopy, and drug loading were used to evaluate the physicochemical characteristics of the nanocrystals. In our study, Abe and Flu were released to exert their synergistic effect when the amide bond of AF was broken and the amide bond was sensitive to cathepsin B which is overexpressed in most tumor tissues, thus increasing the selectivity of the drug to the tumor. The results showed that Lys-PDA@AF NCs had higher cytotoxicity for CRC cell with an IC50 of 4.86 μg/mL. Additionally, pharmacokinetics showed that Abe and Flu had similar absorption rates in the Lys-PDA@AF NCs group, improving the safety of combination therapy. Meanwhile, in vivo experiments showed that Lys-PDA@AF NCs had excellent antitumor effects and safety. Overall, it was anticipated that the created Lys-PDA@AF NCs would be a potential method for treating cancer.
研究领域:
药学
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肿瘤学
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生物材料学
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DOI
PubMed
高影响因子
International Journal of Electrochemical Science
·
2024
·
IF 1.3
Mingjing Yin,Jicheng Yuan,Lina Yu,Xinying Ma,Kun Long,Mingyong Chao
2,3,5,6-Tetramethylpyrazine (TMP), an organic compound with numerous potential health benefits, is extensively used in medical and food products. This research introduces a novel approach for the precise quantification of TMP by employing a poly(L-lysine) modified glassy carbon electrode (pLL/GCE). The pLL/GCE is fabricated through the electrochemical polymerization of L-lysine onto a glassy carbon electrode (GCE) surface. This modification enhances the electrode's electrocatalytic efficiency, resulting in a significant increase in the reduction peak current of TMP. Exploiting this distinctive property, we establish an electrochemical method for quantifying TMP using cyclic voltammetry (CV). Optimized conditions reveal a robust linear correlation between the reduction peak current of TMP and its concentration within two adjacent linearity ranges: 40.0 nmol/L to 1.0 μmol/L and 1.0 μmol/L to 100.0 μmol/L. Notably, this method achieves an impressive detection limit of 20.0 nmol/L. To validate the practicality of this approach, we analyzed various real-world samples, obtaining recovery rates ranging from 98.2% to 100.8%. The developed method holds significant promise for the determination of TMP in medical and food products, underscoring its potential for widespread application.
研究领域:
分析化学
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生物医学工程
·
食品科学
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DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Qian Sun,Xielong Dong,Juan Xu,Ting Wang
The study aimed to develop a multifunctional wound dressing with enhanced antibacterial properties and wound healing promotion. The synthesis process involved preparing oxidized regenerated cellulose (ORC) following a modified procedure, synthesizing chitosan/silver nanoparticles (CS/Ag NPs) via an in-situ reduction method, and subsequently preparing ORC/CS/Lys@Ag NPs hydrogels. Characterization techniques including FTIR, XRD, SEM, and EDS were employed to analyze functional groups, lattice structure, morphology, and elemental composition. Gelation time, swelling behavior, water retention, mechanical properties, viscosity, self-healing capacity, rheological behavior, oxygen permeability, in vitro degradation, release of Ag + , and antibacterial properties were evaluated using various experimental methods. Results indicated that the novel wound dressing has the capability to evenly distribute Ag NPs to effectively counteract bacteria. It can maintain moist conditions for 86 h, resist a sturdy mechanical pressure of 11.3 KPa, and degrade by 11.045 % ± 0.429 within 8 h. Combining its efficient gas exchange abilities, self-repairing function, and biocompatibility, almost full recovery was observed in injured mouse skin within 13 days, highlighting its promising clinical utility.
研究领域:
材料科学
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医学
·
生物工程
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DOI
PubMed
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2024
·
IF 3.3
Kaihua Liu,Zhijie Liu,Junjian Miao,Yiqun Huang,Keqiang Lai
BACKGROUND N ε-carboxymethyllysine (CML), N ε-carboxyethyllysine (CEL) and α -aminoadipic acid (AAA) are important foodborne hazards and their intake can cause a variety of diseases in humans. It is extremely important to investigate the formation mechanism of CML, CEL and AAA, as well as their association with each other when aiming to control their production. RESULTS A multi-response kinetic model was developed within the glucose-lysine Maillard reaction model system. The concentrations of glucose, lysine, glyoxal (GO), methylglyoxal (MGO), CML, CEL and AAA were quantified at different temperature (100–160 °C) and at different intervals (0–60 min). The experimental data were fitted to the proposed model to calculate kinetic parameters for the corresponding steps. The results indicated that the production of CML was primarily relied on the direct oxidative cleavage of the Amadori product, rather than the reaction between GO and Lys, whereas CEL and AAA were generated through the reaction of MGO with Lys. Significantly, the reaction between α -dicarbonyl compounds and Lys preferentially generated CML and CEL, resulting in the lower concentrations of AAA compared to CML and CEL. CONCLUSION The multi-response kinetic model developed in the present study can be applied well to the Maillard reaction. The relationship between the formation mechanisms of CML, CEL and AAA is also explained. © 2024 Society of Chemical Industry.
研究领域:
食品科学
·
生物化学
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PubMed
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2024
·
IF 5.3
Qiannan Wang,Guancheng Jiang,Xingxing Wang,Yinbo He,Lili Yang,Yijie Wang
In the study, JL-BC, an environmentally friendly shale inhibitor with a temperature tolerance of 220 °C was synthesized by grafting modified nano biochar (BC) onto polyarginyl lysine (JL) for the first time. Fourier transform infrared spectroscopy (FTIR) and thermogravimetric (TGA) tests indicated that JL-BC had been successfully synthesized and had good thermal stability. The inhibition performance of JL-BC was experimentally evaluated by linear swelling test, hot-rolling recovery test and sodium bentonite (Na-BT) block immersion test, and the inhibition mechanism of JL-BC was studied by various experimental characterization methods. The experimental results showed that compared with potassium chloride (KCl), polyether amine (PEA), bionic inhibitor dopamine (DA), 2, 3-epoxypropyltrimethylammonium chloride (EPTAC), and poly dimethyl diallyl ammonium chloride (PDMDAAC), Na-BT had the lowest linear swelling height of 4.49 mm in 4 % JL-BC solution at 150 °C. The recovery rates of shale cuttings were highest in 4 % JL-BC solution at 200 °C and 220 °C, which were 96 % and 92 %, respectively. The Na-BT block was immersed in 4 % JL-BC solution for 16 h basically retained its original morphology. The excellent inhibition performance of JL-BC was mainly attributed to the positive charge of JL-BC in aqueous solution at pH 9, which was strongly adsorbed on Na-BT, lowering the zeta potential of Na-BT, destabilizing Na-BT and causing it to aggregate. With the increase of JL-BC concentration, Na-BT gradually aggregated into larger flocs, increasing the particle size of Na-BT. Nano BC reduced water intrusion to a certain extent by physically blocking micropores. In addition, the EC 50 value of JL-BC was 2.93 × 10 5 mg/L, indicating that it was non-toxic. The addition of JL-BC to the soil effectively increased the content of organic matter, ammonium nitrogen and available potassium, and promoted the growth of wheat seedlings. This work may open a new avenue for the development and use of environmentally friendly treatment agents.
研究领域:
材料科学
·
环境科学
·
化学工程
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高影响因子
危险品化学品经营许可证（带存储）