JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2022
·
IF 5.895
Siying Che,Xiutan Peng,Yiwan Zhuge,Xinlan Chen,ChunSong Zhou,Haiyan Fu,Yuanbin She
Detection of pesticide paraquat (PQ) is of considerable significance to ensure food safety, and its rapid and on-site detection is still a challenge. Aimed at the ion characteristics of PQ, an “enrichment and detection” strategy was proposed to improve the sensitivity through electrostatic attractions, and the ion characteristic of probes was adopted to increase the portability through avoiding aggregation-caused quenching effects in the paper strips. Herein, a novel anion-functionalized ionic liquid (IL) probe with a large conjugated plane and rich π-electrons ([Fluo][P66614]2) was designed as a fluorescent and colorimetric dual-channel probe to sensitively and rapidly detect trace amounts of PQ in vegetables and the environment. The proposed probe exhibited good linearity with a detection limit of 64.0 nM in the PQ concentration range of 0.3–7.0 μM (fluorometry) and 0.1 μM in that of 0.1–8.0 μM (colorimetry), respectively. In addition, it displayed a rapid fluorescence quenching response from green to dark (<5 s) and excellent anti-interference (among 23 other pesticides) due to dual effects of electrostatic attraction and π–π stacking. Most importantly, the lipophilic IL probe could be applied in real vegetables and environmental samples with a satisfying recovery rate of 98–103% and assembled into a handy paper strip that achieved the visual semiquantitative detection of PQ. This ionic probe provides a feasible approach for rapidly and conveniently detecting PQ for ensuring agricultural and food safety and opens a new avenue to detect ion-responsive analytes in real complex samples by an “enrichment and detection” strategy.
研究领域:
分析化学
·
食品安全
·
环境科学
✓ 已验证
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2022
·
IF 4.831
Hong Zeng,Hongli Yang,Yue Tang,Xiaojuan Niu,Yuangen Wu
A facile and simple colorimetric biosensor was first established for paraquat (PQ) detection based on the aptamer-enhanced oxidation process of 3,3′,5,5′-tetramethylbenzidine (TMB) by Ag + . The study confirmed that the interaction of PQ-15 aptamer with Ag + accelerates the electron transfer from the aptamer-Ag + complex to dissolved oxygen, which enhances the release of superoxide anion radicals (O 2 ̇ − ) and facilitates the catalytic oxidation of the chromogenic substrate. PQ-15 aptamer will preferentially bind to PQ molecules, resulting in no further enhancement of the catalytic activity of Ag + . Molecular docking results revealed that the PQ molecules are attached to the stem-loop region of the PQ-15 aptamer through σ-π conjugation interactions. The proposed method is simple that only contains Ag + and corresponding aptamer. The limit of detection (LOD) of the constructed colorimetric biosensor for PQ detection was determined to be 16.5 μg·L -1 , belowing the maximum residue limit in fruits and vegetables set by the EU. Moreover, the colorimetric biosensor showed excellent selectivity and anti-interference properties, which was validated for detecting PQ residues in several typical agricultural and water samples.
研究领域:
生物化学
·
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MEDIATORS OF INFLAMMATION
·
2021
·
IF 4.711
Qiang Zheng,Hang Zhao,Dong Jia,Xu Han,Zhenning Liu,Min Zhao
Paraquat (PQ) can cause multiorgan failure including acute kidney injury (AKI). Our prior study showed that Toll-interacting protein (TOLLIP) protected against PQ-induced acute lung injury. However, the role of TOLLIP in PQ-induced AKI remains undefined. This study was aimed at understanding the role and mechanism of TOLLIP in AKI. Six-eight-week-old male Wistar rats were intraperitoneally injected with 25 mg/kg PQ to induce AKI for 24 h in vivo. HK-2 cells were treated with 300 μM PQ for 24 h to induce cellular injury in vitro or 300 μM PQ and 5 μM nuclear factor-κB (NF-κB) inhibitor BAY11-7082 for 24 h. Rats were infected with adenovirus carrying TOLLIP shRNA via tail vein injection and HK-2 cells with adenovirus carrying TOLLIP shRNA or TOLLIP 48 h before PQ exposure. Results showed that TOLLIP and Toll-like receptor 2/4 (TLR2/4) expressions were boosted in the kidney after PQ intoxication. The toxic effect of PQ on the kidney and HK-2 cells was exacerbated by TOLLIP knockdown, as evidenced by aggravated glomerulus and tubule injury, inflammatory infiltration, and cell apoptosis in the kidney and increased loss of cell viability and apoptotic cells in HK-2 cells. TOLLIP knockdown also enhanced PQ-induced NLR family pyrin domain-containing 3 (NLRP3) inflammasome activation in vivo and in vitro and TLR2/4-NF-κB signaling in vitro, reflected by increased contents of proinflammatory cytokines and expressions of NLRP3 inflammasome-related proteins in the kidney and HK-2 cells and expressions of TLR2, TLR4, and nuclear NF-κB p65 in HK-2 cells. However, TOLLIP overexpression inhibited PQ-induced loss of cell viability, cell apoptosis, NLRP3 inflammasome activation, and TLR2/4-NF-κB signaling in vitro. Additionally, BAY11-7082 abolished TOLLIP knockdown-induced NLRP3 inflammasome activation in vitro, indicating that TOLLIP protected against NLRP3 inflammasome activation in PQ-induced AKI through inhibiting TLR2/4-NF-κB signaling. This study highlights the importance of TOLLIP in AKI after PQ intoxication.
研究领域:
药理学
·
毒理学
·
免疫学
·
肾病学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Fuyou Du,Lingshun Sun,Qiulian Zen,Wei Tan,Zhenfang Cheng,Guihua Ruan,Jianping Li
A simple and rapid fluorescence “on-off-on” approach for sensing Hg 2+ and paraquat was designed by using nitrogen doped graphene quantum dots (N-GQDs) and N-GQDs/Hg 2+ system, respectively. It was found that the fluorescence of N-GQDs solution was quenched in the presence of Hg 2+ ions (turn off), and the quenched N-GQDs/Hg 2+ system was recovered after addition of paraquat (turn on), due to the higher affinity of paraquat and Hg 2+ than that of N-GQDs and Hg 2+ that resulting into the release of Hg 2+ from the surface of N-GQDs and the fluorescence recovery of N-GQDs. The developed fluorescence “on-off-on” approach exhibited high selectivity and sensitivity for detection of Hg 2+ and paraquat. Under the optimum experimental conditions, good linearities were achieved over the range of 0–4.31 μM for Hg 2+ and 0.05–2.0 μg mL −1 for paraquat, respectively. The detection limits for Hg 2+ and paraquat were 23 nM and 19 μg L −1 , respectively, and the recoveries of Hg 2+ and paraquat spiked in real samples ranged from 95% to 105.8%. Results of this study suggested that the highly selective and sensitive fluorescence “on-off-on” approach can be used to the detection of Hg 2+ and paraquat in real environmental water samples.
研究领域:
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
Foods
·
2022
·Vol. 11
·期 16
·2405
·
IF 5.561
Qiaoling Wu,Han Tao,Yuangen Wu,Xiao Wang,Qili Shi,Donglin Xiang
Paraquat (PQ) has high acute toxicity, even at low concentrations. For most people, the main pathway of exposure to PQ is through the diet. Therefore, the development of simple and efficient methods for PQ testing is critical for ensuring food safety. In this study, a new electrochemical detection strategy for paraquat is proposed based on the specific binding of PQ to its nucleic acid aptamer. Firstly, the Zn/Fe bimetallic ZIF derived nanoporous carbon (Zn/Fe-ZIF-NPC) and nickel hexacyanoferrate nanoparticles (NiHCF-NPs) were sequentially modified onto the glassy carbon electrode (GCE). NiHCF-NPs served as the signal probes, while Zn/Fe-ZIF-NPC facilitated electron transfer and effectively enhanced the sensing signal of NiHCF-NPs. Au nanoparticles (AuNPs) were then electrodeposited on the NiHCF-NPs/Zn/Fe-ZIF-NPC/GCE and then the thiolated aptamer was assembled on the AuNPs/NiHCF-NPs/Zn/Fe-ZIF-NPC/GCE via Au-S bonding. When incubated with PQ, the formation of PQ–aptamer complexes delayed the interfacial electron transport reaction of NiHCF-NPs, which caused a decrease in the current signals. As a result, simple and highly sensitive detection of PQ can be readily achieved by detecting the signal changes. A linear range was obtained from 0.001 to 100 mg/L with a detection limit as low as 0.34 μg/L. Due to the recognition specificity of the aptamer to its target molecule, the proposed method has excellent anti-interference ability. The prepared electrochemical aptasensor was successfully used for PQ assay in lettuce, cabbage and agriculture irrigation water samples with recoveries ranging from 96.20% to 104.02%, demonstrating the validity and practicality of the proposed method for PQ detection in real samples.Keywords:paraquat;Zn/Fe bimetallic MOF;nanoporous carbon;electrochemical aptasensor;label-free;food safety
研究领域:
分析化学
·
环境科学
·
食品安全
✓ 已验证
产品关联
DOI
PubMed
高影响因子
NEUROSCIENCE RESEARCH
·
2011
·
IF 2.096
Haiyan Xu,Ruirui Chen,Xiaoyan Cai,Defu He
Neostriatum plays an important role in the pathophysiology of Parkinson's disease (PD). However, the changes of sensitivity of dopamine receptors of neostriatal neurons in PD have been less addressed in vivo . In the present study, systemic exposure to paraquat and maneb induced Parkinsonian symptoms and neuronal loss of substantia nigra pars compacta. Using single-unit recording methods, three types of neostriatal neurons were recorded including medium spiny-like neurons, large aspiny-like neurons and fast-spiking interneurons. In the exposed rats, increased firing activity of neostriatal neurons was revealed when compared to control rats. Following D1 receptor agonist, SKF38393 and D2 receptor agonist, LY171555 iontophoretically administrated respectively, effects of increase and decrease in firing activity were both observed in neostriatal neurons. However, stronger inhibitory effects of activating D1 receptors and weaker excitatory effects of activating D2 receptors were found in the exposed rats as compared to controls. It indicated that differential changes of sensitivity of D1 and D2 receptors in Parkinson's disease were related to the modulation of the imbalance between D1-receptor-dependent striatonigral direct pathway and D2-receptor-dependent striatopallidal indirect pathway. Our results illustrate the electrophysiological changes of in vivo neostriatal neurons in Parkinson's disease, thereby providing insight into the regulatory mechanisms of dopamine-mediated physiology.
研究领域:
神经科学
·
药理学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2019
·
IF 2.813
Shengdong Pan,Jiabin Zhang,Qian He,Xiaohong Chen,Micong Jin
In this study, novel benzenesulfonic acid groups modified magnetic microspheres (Fe 3 O 4 @SiO 2 @poly(4-VB)) were synthesized and characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier-transform infrared spectrometry (FTIR), and vibrating sample magnetometer (VSM). The as-prepared Fe 3 O 4 @SiO 2 @poly(4-VB) was employed as a magnetic-phase extraction (MSPE) adsorbent for rapid determination of paraquat (PQ) and diquat (DQ) in human urine samples coupled with ultra-high performance liquid chromatography-high resolution mass spectrometry (UPLC-HRMS). Moreover, this paper had expounded systematically the mass spectrum cracking mechanisms of PQ and DQ. And a zwitterionic functionalized SIELC Obelisc R column was employed for separation and retention of the above two polar herbicides using 50 mmol/L ammonium formate (pH = 3.7)-acetonitrile as the mobile phase. Besides, the adsorption and desorption conditions of Fe 3 O 4 @SiO 2 @poly(4-VB) toward PQ and DQ were optimized in spiking urine samples to obtain the best adsorption and desorption efficiencies. And the adsorption mechanisms of Fe 3 O 4 @SiO 2 @poly(4-VB) toward PQ and DQ referred to synergetic effect of electrostatic attraction and π-π interaction. Under the optimal conditions, the inter-day and intra-day spiking recoveries of the proposed method were in the range of 86.7–109.9% with RSDs less than 10%. The limits of detection (LODs) were obtained by spiking in blank urine samples at a series of low concentrations and were found to be 0.12 μg/L and 0.14 μg/L for PQ and DQ, respectively, which were lower than the comparing literatures. The developed analytical method was proven to be simple, rapid, sensitive, and accurate for clinical poisoning analysis.
研究领域:
分析化学
·
毒理学
·
临床医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2023
·
IF 6.1
Shuiping Tang,Yong Huang,Shulin Zhao,Kun Hu
Herein, a novel surface molecularly imprinted-based matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (SMI-MALDI-TOF MS) method for direct target paraquat (PQ) analysis in complicated samples is reported. Notably, a captured analyte-imprinted material can be directly detected via MALDI-TOF MS by using imprinted material as nanomatrix. Using this strategy, the molecular specific affinity performance of surface molecularly imprinted polymers (SMIPs) and the high-sensitivity detection capability of MALDI-TOF MS was integrated. The introduction of SMI endowed the nanomatrix with the capacity for rebinding the target analyte and ensuring specificity, prevented the interfering organic matrix, and enhanced the analyzing sensitivity. By using paraquat (PQ) as a template, dopamine as a monomer , and covalent organic frameworks with a carboxyl group (C-COFs) as a substrate, polydopamine (PDA) was decorated on C-COFs via a simple self-assembly procedure to generate an analyte-based surface molecularly imprinted polymer (C–COF@PDA-SMIP), which served the dual function of SMIP capturing the target analytes and high-efficiency ionization. Thus, a reliable MALDI-TOF MS detection PQ with high selectivity and sensitivity as well as an interference-free background was achieved. The synthesis and enrichment conditions of C–COF@PDA-SMIPs were optimized, and its structure and property were characterized. Under optimal experimental conditions, the proposed method achieved highly selective and ultrasensitive detection of PQ from 5 to 500 pg mL −1 , and the limit of detection was as low as 0.8 pg mL −1 , which is at least three orders of magnitude lower than that achieved without enrichment. In addition, the specificity of the proposed method was superior to that of C-COFs and nonimprinted polymers. Moreover, this method exhibited reproducibility, stability, and high salt tolerance. Lastly, the practical applicability of the method was successfully verified by analyzing complicated samples, such as grass and orange.
研究领域:
分析化学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2022
·
IF 4.601
Shengdong Pan,Zhiping Huang,Chunqing Shang,Li Wang,Qiaoli Qiu,Zemin Xu,Dandan Zhang
In cases of low concentration paraquat (PQ) poisoning, as the disease progresses over a long period of time, the prototype PQ may not be detected in the urine, which has a significant negative impact on the precise treatment of the poisoning. But PQ poisoning can also be confirmed by the detection of specific metabolites of PQ in the urine samples. In the present study, core-shell amphiphilic carboxyl-functionalized magnetic polymer microsphere (Amphiphilic-MPs-COOH) was prepared, and the as-prepared Amphiphilic-MPs-COOH was characterized by transmission electron microscope (TEM), scanning electron microscope (SEM), Fourier-transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), and thermogravimetry and differential thermogravimetry analyses (TG-DTG). Then, the Amphiphilic-MPs-COOH was employed as a magnetic solid-phase extraction (MSPE) adsorbent for pretreatment and rapid determination of PQ and its four metabolites from urine samples prior to ultra-high performance liquid chromatography-high resolution mass spectrometry (UPLC-HRMS). The extraction-elution conditions of Amphiphilic-MPs-COOH towards PQ and its metabolites were optimized in spiking urine samples to obtain the best MSPE efficiency. The adsorption mechanism of PQ and its metabolites by Amphiphilic-MPs-COOH involves electrostatic attraction and π-π stacking interactions. Moreover, the effect of different ratios of hydrophilic monomer NVP and functional monomer 4-VBA on the extraction and purification performance of PQ and its metabolites in urine samples. And the results revealed that both hydrophilic monomer and functional monomer were important for the adsorption of PQ and its metabolites, and the addition of the appropriate amount of the hydrophilic monomer NVP can improve the compatibility of the adsorbent with the urine substrate. In addition, this study compared the matrix effect of the Amphiphilic-MPs-COOH based MSPE method and the commercial Waters Oasis WCX SPE method. The results showed that the Amphiphilic-MPs-COOH based MSPE method developed in this paper had better resistance to matrix interference. Under optimal conditions, the recoveries of PQ and its metabolites were ranging from 84.5 to 103%, with relative standard deviations (RSDs) of 1.1–6.3%. While the limits of detection (LODs, S/N ≥ 3) and limits of quantification (LOQs, S/N ≥ 9) of the method were in range of 0.1–1.6 μg/L and 0.3–4.8 μg/L, respectively. Finally, the established MSPE-UPLC-HRMS method in this study was used to confirm PQ poisoning not only based on detecting PQ prototype, but also on its four metabolites, providing strong technical support for clinical precision treatment.
研究领域:
分析化学
·
毒理学
·
临床医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）