查找使用阿拉丁产品的科研文献

Targeted Treatment of Myocardial Infarction by Macrophage Membrane Coated with Resveratrol Nanoparticles

Tongli Li,Jintang Feng,Wenyu Ding,Zhang Zhang

Myocardial infarction (MI) is characterized by rapid onset and high mortality. Due to the complexity of its pathological process, targeted therapy for MI has been a long-standing challenge. Macrophages, as immune cells, selectively accumulate in the infarct area after MI. This inherent targeting ability, coupled with excellent immune evasion capabilities, makes macrophages an ideal source for targeted delivery to damaged myocardium as a biomimetic delivery carrier. Therefore, in this work, we aimed to develop biomimetic nanoparticles for specific targeted drug delivery to efficiently treat MI. Using cell membrane-coated nanotechnology, we prepared resveratrol-loaded nanoparticles (RESNPs) using poly(lactic-co-glycolic acid) (PLGA) nanoparticles, and then coated the surface of RESNPs with macrophage membranes to prepare biomimetic nanoparticles MM/RESNPs. We then described the physical properties of MM/RESNPs, and their targeting, therapeutic effects, and safety were evaluated in a MI mouse model. The results show that MM/RESNPs exhibited good biocompatibility and could effectively target damaged myocardial sites. MM/RESNPs were found to considerably improve cardiac function and reduce the infarct size in MI mice. Furthermore, MM/RESNPs demonstrated good biosafety after long-term administration. Macrophage membrane-coated biomimetic nanoparticles (MM/RESNPs) exhibit excellent nanoperformance and biosafety, enabling targeted drug delivery and achieving safe and effective treatment for MI.

Resveratrol/POSS-based polymethacrylates for DLP 3D printing and fluorescent anti-counterfeiting

Wenxiu Chen,Youzhi Zeng,Zaoxia Wen,Linxuan Fang,Lianbin Wu

A trifunctional methacrylate monomer (REM) was synthesized from biomass resveratrol to address the need for sustainable resins in digital light processing (DLP) 3D printing. The polymer properties were optimized by incorporating methacryloxypropyl polyhedral oligomeric silsesquioxane (MA-POSS) with different mass fractions. Light-curing biobased materials (REM/POSS) exhibited enhanced thermal stability and dielectric properties. The REM/POSS-30 % composite showed a low dielectric constant ( D k  = 2.90 at 14.2 GHz) and excellent thermal stability. Compared to commercial bisphenol A-based resins (MEP), REM/POSS-0 % demonstrated superior comprehensive performance. The stilbene structure of resveratrol in REM/POSS polymers provided strong blue fluorescence under UV light, suggesting potential for information storage and encryption. The successful fabrication of complex structures confirmed the compatibility of REM/POSS with DLP 3D printing, offering a promising alternative to petroleum-based materials in both 3D printing and anti-counterfeiting applications.

Encapsulation of resveratrol by Pickering emulsion stabilized by Pueraria lobata nano-starch

Wei Ding,Rui Wu,Jie Guo,Xianwu Zhou,Shima Liu,Jian He,Ke Song

Resveratrol is a naturally occurring polyphenol with pronounced bioactivity. Its cardioprotective, anti-inflammatory, and antioxidant properties confer broad potential for disease prevention and therapy. However, its poor aqueous solubility and limited stability constrain practical applications. In this study, Pickering emulsions stabilized by octenyl succinic anhydride (OSA)-modified starch nanoparticles (OSA-SNPs) were developed to enhance the physicochemical stability and bioavailability of resveratrol. SNPs were prepared from Pueraria lobata starch by antisolvent precipitation using ethanol and were subsequently modified with OSA to yield OSA-SNPs. Starch-based Pickering emulsions stabilized with OSA-SNPs were formulated for resveratrol encapsulation. The encapsulation efficiency, droplet size, and emulsion stability were characterized. Stable emulsions with an encapsulation efficiency of 92.16 % were obtained. Compared with surfactant-stabilized systems, the starch-based Pickering emulsions exhibited superior resistance to Ostwald ripening and improved biocompatibility. In vitro simulated gastrointestinal digestion studies demonstrated a sustained-release profile of resveratrol over time. These findings indicate that OSA-modified Pueraria lobata starch nanoparticles are a promising green stabilizer for delivery systems of poorly soluble and labile bioactive compounds, offering a practical strategy for their application.

Resveratrol ameliorates DSS-induced ulcerative colitis by acting on mouse gut microbiota

Yu Bing,Wang Ying,Tan Zekai,Hong Zhengyi,Yao Lewen,Huang Shiyuan,Li Zexi,Zhang Lanyue,Li Hui

Objective and design Ulcerative colitis (UC) is a multi-faceted, recurrent immune disorder caused by dextran sulfate sodium (DSS). The intestinal microbiota has multiple functions in the host, so UC requires long-term potent medication. The effect of resveratrol (RSV) has seldom been reported, and this study researched that. Herein, the effect of RSV and Grape seed oil that anti-inflammatory ability in experimental mice was explored, also why RSV altered Gut Microbiota has been researched. Materials and methods In this experiment, the effects of experimental drugs on colon length in mice with DSS-induced colitis were compared. H&E Staining was performed on serial sections of colon tissues and histological scores were determined for all groups. The expression of cyclooxygenase-2 (COX-2) and tumor necrosis factor-α (TNF-α) in the colon tissue of mice was detected by immunohistochemical staining. In the end, the α-diversity index, sobs index, and rarefaction curve of the cecal and colon microbiota of different groups of mice were measured. Bray–Curtis-based Venn diagram of PCoA (principal coordinate analysis) and OTUs distribution in mouse gut microbiota were obtained. Results The results showed that the use of 40 mg/kg RSV (high dose) significantly reduced the severity of UC. The use of 10 mg/kg RSV (low dose) significantly reduced the effect of shortened colon length in DSS mice. Compared with the DSS-treated group, the levels of COX-2 and TNF-α in the colon tissues of RSV + DSS-treated mice were significantly decreased. According to this experiment, 19 mouse gut microbiota species had a relative abundance greater than 0.1%, with Beerella, Bacteroides, Helicobacter, Oscillator, and cecum pylori being more abundant in the colon than in the colon. A higher relative abundance of Lachnospira NK4A136 was observed in DSS and RSV groups compared with the control group, whereas the opposite was observed for Alloprevotella. This proves that resveratrol increases the uniformity and diversity of gut microbes to a certain extent, and has a protective effect on the gut.

Efficacy of a resveratrol nanoformulation prepared by a facile solvent-free method

Jiahao Li,Yushuang Wei,Qin Lai,Xiangyang Li,Yu Wang,Xun Wang,Yinghua Chen,Hong Liu,Kai Yang,Bing Yuan

Resveratrol (RSV) is a natural polyphenolic compound known for its antioxidant, anti-inflammatory, anticancer, and cardioprotective properties. However, the limited water solubility and poor bioavailability of RSV significantly hinder its applications in the food and pharmaceutical industries. To address these challenges, we developed a facile, scalable, and specifically organic-solvent-free method to prepare highly stable and concentrated RSV nanoformulations. By utilizing an ethoxylated hydrogenated castor oil (EHCO) aqueous solution, we successfully dissolve RSV in water at concentrations up to 30 mg/mL. This RSV solution can subsequently be incorporated with hydrogenated lecithin S10 to formulate stable lipid nanoparticles (e.g., 140–180 nm). The entire process is performed under heating and stirring, eliminating the need for organic solvents and ensuring simplicity and high reproducibility. The resulting RSV-CO60@S10 nanoformulations exhibit relatively high encapsulation efficiency (with final RSV concentrations up to 30 mg/mL), long-term stability (exceeding 6 months), preserved antioxidant activity, and effective cellular internalization capabilities that alleviate oxidative stress. Additionally, these nanoparticles exhibit promising therapeutic efficacy on atopic dermatitis in mice. These findings offer valuable insights into the potential utilization of RSV across diverse applications.

The anti-depressant effects of a novel PDE4 inhibitor derived from resveratrol

Yingcong Yu,Jinhui Wang,Xianfeng Huang

Preparation of Hyaluronic Acid-Coated Resveratrol Nanomicelles and Its In Vitro Evaluation

Xia J.,Li X.,Lv C.,Jiang J.,Zhu T.,Liu T.,Li J.,Zhang W.

Abstract Resveratrol (Res), a non-flavonoid polyphenolic compound extracted from natural plants, has many pharmacological effects such as anti-tumor, organ protection, improvement of cardiovascular disease, and anti-inflammation. However, due to its poor water solubility and light-degradable property, its bioavailability is restricted. In this study, the hyaluronic acid (Ha)-coated poloxamer 188 (F68) and poloxamer 407 (F127) and Ha-coated Res nanomicelles (Ha-F68/F127-Res-Mc) were prepared by film dispersion method. The nanomicelles were evaluated by indicators of encapsulation efficiency ( EE ), drug loading ( DL ), particle size, polydispersity index and ζ-potential. Besides, using the Box–Behnken design (BBD) based response surface methodology (RSM), obtained the optimum prescription process to obtain the Ha-F68/F127-Res-Mc. Compared with the Res active pharmaceutical ingredient (API), Ha-F68/F127-Res-Mc had higher water solubility and photostability, and could achieve sustained release. Compared with the Res active pharmaceutical ingredient (API), Ha-F68/F127-Res-Mc had higher water solubility and photostability, and could achieve sustained release. In conclusion, the prepared nanomicelles have stronger cytotoxic effect on MCF-7 than doxorubicin (DOX). This will provide a basis for the development of Res antitumor injection, and provide a reference for the research on nano-delivery systems of other BCS II drugs in the future.

Modification and stabilizing effects of PEG on resveratrol-loaded solid lipid nanoparticles

Su Jun-Qing,Wen Zhen,Wen Yi-Ai,Xiao Wan-Ni,Lin Jing,Zheng Zong-Kun

Resveratrol-loaded solid lipid nanoparticles (SLNs) modified by polyethylene glycol (PEG2000) (RES–PEG–SLNs) were prepared to study the stabilizing influences of PEG2000 on SLNs properties including loading capacity, particle size, photostability, and release. The micromorphology, particle size distribution, drug–lipid–modifier interaction and crystalline structure were characterized to elucidate stabilizing effects of PEG2000 on SLNs. Compared with ordinary SLNs, SLNs modified by PEG2000 at relatively low amounts of [ m (PEG2000): m (lipids) = 1:10] exhibit high drug loading, steady nanoparticle size distributions, photostability and sustained release. According to characterizations, RES–PEG–SLNs formation is dependent on the physical interactions of drug–lipid–modifier. Since PEG2000 is doped into lipid matrix in a non-crystalline state, the lipids crystalline arrangement is disrupted. Additionally, RES–PEG–SLNs are crystallized in a PEG2000/lipid eutectic mixture rather than a simple mixture, inhibiting the lipid polymorphism transformation from α - to β -form, and therefore preventing drug exclusion from the lipid matrix. The PEG2000/lipid matrix contains lattice defects, which allow for the incorporation of more resveratrol and preventing it from photodegradation effectively. In contrast to the burst release of SLNs modified without PEG2000, resveratrol is released more slowly from the lattice defects in lipid matrix of RES–PEG–SLNs, resulting in a sustained release fitted by a two-stage exponential kinetic equation. PEG2000 is distributed on the RES–PEG–SLNs surface, increasing repulsion between nanoparticles and avoiding particles aggregation. These results confirm that both matrix doping effects and surface steric hindrance produced by the presence of PEG2000 play important roles in maintaining high loadings, nanoparticle size, photostability and sustained release.

Dissolving microneedle patches loaded with resveratrol albumin nanoclusters for corneal anti-angiogenic therapy

Yingrong Chen,Lu Wang,Renjie Wu,Jiawei Zhu,Weili Zhang,Yuepiao Cai,Tao Xue,Xiang Yu

Corneal neovascularization (CNV) is a leading cause of corneal blindness. Although resveratrol (RES) effectively inhibits neovascularization, its clinical utility remains severely limited by poor aqueous solubility and low permeability across ocular surface barriers. To address these challenges, we developed dissolving microneedle patches encapsulating RES-loaded bovine serum albumin nanoclusters (RES-NC@MN). Specifically, we fabricated RES-NCs via a microfluidic-assisted nanoprecipitation method and systematically optimized the formulation using a three-factor Box-Behnken design. The optimized RES-NCs exhibited uniform spherical nanostructures (∼158 nm) with high encapsulation efficiency. XRD and DSC analyses confirmed the amorphous state of the encapsulated drug. The resulting RES-NC@MNs demonstrated sufficient mechanical strength for corneal insertion and facilitated rapid drug release. Notably, the steady-state flux of RES from RES-NC@MNs was approximately 10-fold higher than that from RES suspensions. Pharmacokinetic studies revealed that RES-NC@MNs achieved a 7.2-fold increase in C max and a 26.5-fold greater AUC 0-∞ compared to conventional RES suspensions. In a rat alkali-burn model, treatment with these MNs reduced the neovascular area by approximately 70% and significantly suppressed CD31 and VEGF expression alongside pro-inflammatory cytokine levels. Consequently, the RES-NC@MN system presents a robust strategy for delivering lipophilic therapeutics to the ocular surface, offering enhanced anti-angiogenic efficacy for CNV treatment.

Ovalbumin-proanthocyanidin‑sodium alginate conjugate-based oleogels: Construction, characterization and resveratrol delivery performance

Zenghong Xing,Xiaoyun Fei,Deming Gong,Guowen Zhang

Resveratrol (Res) is a bioactive compound with limited application in food and pharmaceuticals due to its poor solubility, low stability and limited bioavailability. To address these challenges, this study developed novel ovalbumin-proanthocyanidin‑sodium alginate (OVA-PC-SA) conjugate-based oleogels, and investigated the effect of conjugate concentration on the properties of oleogels and Res's delivery performance. Results indicated that oil binding capacity exceeded 99% at conjugate concentrations of 0.4–1.0%, and the hardness, gel strength and thermal stability of the oleogels were enhanced significantly with increasing conjugate concentration. All oleogels contained β-type crystals and exhibited superior oxidative stability compared to unstructured oil. At conjugate concentrations of 0.2% and 1.0%, the oleogels exhibited high encapsulation efficiencies (87.47% and 91.10%), loading capacities (99.10 and 102.23 mg/g), and significantly improved Res's in vitro bioaccessibility (80.53% and 85.06%) compared to Res-loaded oil (75.41%). The oleogels enhanced the controlled release of lipophilic bioactive substances, demonstrating their potential as effective carriers.

Development and application of resveratrol-encapsulated nanostructured lipid carriers for mulberry juice fortification

Lukai Ma,Zijian Li,Guoqin Liu,Gengsheng Xiao,Linlin Li,Shuo Wan

Resveratrol-loaded NLCs (Res-NLCs) were developed in this study. • Res-NLCs improved the solubility and bioaccessibility of Res. • Res-NLCs exhibited enhanced stability under acidic and thermal stress conditions. • In mulberry juice, Res-NLCs stabilized anthocyanins, enhancing bioaccessibility. • Res-NCLs have potential to enhance the quality of functional beverages. The physiological efficacy of resveratrol (Res) is limited by its inherent hydrophobicity and poor bioaccessibility. Nanostructured lipid carriers (NLCs) have been engineered to overcome these pharmacokinetic bottlenecks. In this study, Res-NLCs were developed, characterized byuniform spherical morphology and high encapsulation efficiency (99.07%). Res-NLCs maintained relatively stable physicochemical properties under the evaluated storage conditions and exhibited enhanced intestinal transport potential, with an apparent permeability coefficient of 4.84 × 10 −6  cm/s. In a food matrix application (mulberry juice), the Res-NLCs acted as a robust delivery vehicle and a stabilizer for endogenous anthocyanins. Notably, the Res-NLCs increased anthocyanin storage retention by 7.28% and reduced anthocyanin loss by 283.9 mg/L. Dynamic digestion further confirmed that Res-NLCs protected the anthocyanins during the intestinal phase and modulated their release, thereby improving their potential bioaccessibility. These findings highlight the potential of Res-NLCs as multifunctional ingredients to enhance the nutritional profile and chemical stability of functional beverages. Download: Download high-res image (183KB) Download: Download full-size image

Optimization of Polydatin Hydrolysis Process Through Response Surface Methodology for Efficient Resveratrol Production

Dong Wang,Yating Xiao,Xia Yang,Jie Cui,Yangyang Cai,Hua Chen

Background/Objectives:Resveratrol is a naturally occurring polyphenolic stilbene compound exhibiting a wide range of biological activities, and it has been extensively utilized as both a food additive and a pharmaceutical active ingredient. Typically, it can be directly extracted from natural sources such as grapes, mulberries, and peanuts, or obtained through catalytic hydrolysis of polydatin. To establish an efficient and optimized method for resveratrol production, we conducted a comprehensive study to refine the acid-catalyzed hydrolysis conditions of polydatin.Methods:A high-performance liquid chromatography method was developed for the quantitative determination of polydatin and resveratrol. To identify the optimal ranges of reaction temperature, HCl concentration, and ethanol concentration, single-factor experiments were conducted by evaluating their influences on hydrolysis kinetics and resveratrol yield. Based on these results, response surface methodology incorporating a Box–Behnken design was employed to optimize the hydrolysis process, using resveratrol yield as the response variable. Furthermore, time-course experiments were performed to determine the optimal reaction duration under the established optimal conditions.Results:Single-factor experiments demonstrated that increasing temperature and HCl concentration significantly accelerated hydrolysis, but resveratrol yield increased initially and then decreased with excessive increases in either factor. To further optimize the process, response surface methodology optimization experiments were conducted at temperatures of 60, 70, and 80 °C; HCl concentrations of 1.0, 1.5, and 2.0 M; and ethanol concentrations of 75%, 85%, and 95%. The optimal conditions were identified as follows: temperature, 70 °C; HCl concentration, 1.5 M; ethanol volume fraction 85%; and reaction time, 5 h. Under these conditions, the theoretical resveratrol yield was 85.68%, and the average yield from triplicate validation experiments was 86.01% (RSD = 0.56%), which was consistent with the theoretical value.Conclusions:The optimized acid-catalytic hydrolysis process using RSM is stable, feasible, and efficient, offering a promising approach for enhancing resveratrol production from polydatin.