Microorganisms
·
2025
·Vol. 13
·期 11
·2554
·
IF 4.2
Yong-Lan Ma,Min Li,Qian Lei,Hai-Jun Ma,Ya-Jing An
Auxenochlorella pyrenoidosa , a promising edible bioresource, can be efficiently and safely cultivated using exogenous phytohormones to enhance its productivity. This study employed multi-omics analysis to systematically investigate the effects and mechanisms of exogenous trans-Zeatin (tZ) on the growth and metabolism of A. pyrenoidosa . Results demonstrated that 10 mg/L tZ significantly promoted algal growth, increasing biomass by 166 ± 3.35% at 72 hours (h), while concurrently elevating cellular soluble protein (SP), carbohydrate (CHO), and chlorophyll a (Chla) content. tZ also strengthened the antioxidant defense system, evidenced by reduced reactive oxygen species (ROS) levels, enhanced activities of antioxidant enzymes (superoxide dismutase (SOD) and catalase (CAT)), upregulation of glutathione metabolism, and decreased lipid peroxidation product (malondialdehyde (MDA)). Furthermore, tZ activated key metabolic pathways, including nitrogen metabolism, photosynthetic carbon fixation, and porphyrin biosynthesis, leading to the accumulation of arginine and polyamines, etc. This study reveals that tZ promotes microalgal growth by coordinately regulating carbon–nitrogen metabolic networks and antioxidant systems, providing a theoretical foundation for phytohormone-augmented microalgae cultivation technologies.
研究领域:
藻类学
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植物生理学
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代谢组学
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分子生物学
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生物技术
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PubMed
高影响因子
ACS Nano
·
2025
·
IF 16
Jingkai Wang,Jiangjie Chen,Jinyang Chen,Kaishun Xia,Pengcheng Yuan,Xiaopeng Zhou,Ronghao Wang,Chao Yu,Yi Li,Kesi Shi,Yuang Zhang,Haibin Xu,Tengfei Zhao,Hao Li,Qixin Chen,Shiqing Feng,Chengzhen Liang
The central nervous system (CNS) barrier obstructs therapeutic component entrance and hinders the therapy efficiency of CNS diseases. An ideal delivery system should penetrate and concentrate in the CNS without safety concerns. Nanovesicles (NVs) are a popular delivery tool, because of their biological homology, inherent homing effects, and capacity to penetrate barriers. However, the delivery efficacy of NVs is insufficient for CNS disease therapy, and the mechanism for barrier penetration remains elusive. Herein, nanovesicles (NVs) were extruded from mesenchymal stem cells and modified by a lesion tissue affinity peptide (CAQK) for spinal cord injury (SCI) therapy. The NVs penetrated endothelial barriers effectively in vitro. Subsequently, the CNS barrier penetration capacity of the CAQK-conjugated NVs (CNVs) was verified in vivo in spinal cord injury (SCI) and the temporary middle cerebral artery occlusion (t-MCAO) mouse models. Furthermore, the endothelial barrier penetration of CNVs depended on the active endocytosis by endothelial cells. After endocytosis, the Rab11+ endosome was identified to mediate a transcellular transcytosis to transport CNVs across the barrier. In the SCI model, CNVs promoted the lesion tissue accumulation, leading to improvement in the neural functional recovery. In summary, we developed a natural NV tool for SCI therapy, employing the inherent CNS barrier penetration capacity and enhanced lesion tissue homing characteristics of NVs. The NVs crossed the CNS barriers via active endocytosis, followed by Rab11+ endosome-mediated transcytosis. The CNV exhibited good delivery efficacy and therapeutic effects in CNS diseases and has the potential for clinical translation.
研究领域:
纳米医学
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神经科学
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药物递送系统
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再生医学
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生物医学工程
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DOI
PubMed
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BIOCHEMICAL PHARMACOLOGY
·
2023
·
IF 5.8
Chaofeng Xing,Minyi Tang,Jianqin Yang,Shuai Wang,Qihua Xu,Wenbin Feng,Yunping Mu,Fanghong Li,Allan Zijian Zhao
Type 1 diabetes mellitus (T1DM) is characterized by life-threatening absolute insulin deficiency . Although ω-3 polyunsaturated fatty acids (PUFAs) displayed significant anti-hyperglycemic activity, the insulinotropic effects of their metabolites remain unknown. In this study, we took advantage of a transgenic model , mfat-1, that overexpresses an ω-3 desaturase and can convert ω-6 PUFAs to ω-3 PUFAs. Eicosapentaenoic acid (EPA) was sharply elevated in the pancreatic tissues of mfat-1 transgenic mice compared with wild-type (WT) mice. In contrast to the WT mice, the mfat-1 transgenics did not develop overt diabetes and still maintained normal blood glucose levels and insulin secretion following streptozotocin-treatment. Furthermore, under the condition of pancreatic β-cell damage, co-incubation of the metabolites of EPA produced from the CYP 450 pathway with isolated islets promoted the overexpression of insulin as well as β-cell specific markers, pdx1 and Nkx6.1 in pancreatic α-cells. Addition of EPA metabolites to the cultured glucagon-positive α-cell lines, a series of pancreatic β-cell markers were also found significantly elevated. Combined together, these results demonstrated the effects of ω-3 PUFAs and their metabolites on the trans-differentiation from α-cells to β-cells and its potential usage in the intervention of T1DM.
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JOURNAL OF MOLECULAR CATALYSIS A-CHEMICAL
·
2014
·
IF 3.679
Yujia Xie,Fengyong Zhang,Pingle Liu,Fang Hao,He’an Luo
Metalloporphyrin-catalysed direct functionalization of saturated C H bonds is one among the topical and challenging areas within the chemical industry. In this work, three types of unsupported and boehmite supported trans-A 2 B 2 -metalloporphyrin chloride complexes (Co-D( p -Cl)PPCl, Fe-D( p -Cl)PPCl, Mn-D( p -Cl)PPCl) were prepared and characterized. It has been found that these trans-A 2 B 2 -metalloporphyrins have high catalytic activity in cyclohexane oxidation under dioxygen. The boehmite supported metalloporphyrins show better catalytic performance and are more stable than unsupported metalloporphyrins. Among these supported metalloporphyrin catalysts, Co-D( p -Cl)PPCl/BM was better than Fe-D( p -Cl)PPCl/BM and Mn-D( p -Cl)PPCl/BM, the cyclohexane conversion and selectivity to KA oil are 6.83% and 83.30%, respectively, and the turnover number is 1.05 × 10 7 . The Co-D( p -Cl)PPCl/BM catalyst can be facilely recovered and was recycled up to six times without significant decrease in catalytic performance.
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ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2016
·Vol. 23
·期 23
·23422-23435
·
IF 2.76
Li Qinchun,Yang Xiaoyan,Wang Hongbin,Wang Haijuan,He Shujuan
A field survey was conducted to evaluate soil metal pollution and endogenous trans -zeatin content in the leaves of plants growing at six sites in a metal-polluted area located in Gejiu, Yunnan, China. Five plant species were collected, and the physicochemical properties and concentrations of five metals in the soil were analyzed. The trans -zeatin content in plant leaves was measured by high-performance liquid chromatography. Based on the Nemerow pollution index, the six sites were classified into four levels of pollution (i.e., low, medium, high, and severely high). The degree of soil metal pollution was cadmium (Cd) > arsenic (As) > lead (Pb) > zinc (Zn) > copper (Cu). The leaf trans -zeatin content in Pteris vittata (an arsenic hyperaccumulator) increased significantly by 98.6 % in soil with a severely high level of pollution compared with soil at a low level of pollution. However, in non-hyperaccumulators Bidens pilosa var . radiata and Ageratina adenophora , a significant decrease in leaf trans -zeatin content of 35.6 and 87.6 %, respectively, was observed. The leaf trans -zeatin content in Artemisia argyi also decreased significantly by 73.6 % in high metal-polluted soil compared with that in medium metal-polluted soil. Furthermore, significant correlations were observed between leaf trans -zeatin content in Pteris vittata and As, Pb, and Cd concentrations in the soil; however, either no correlation or a negative one was observed in the other plant species. Therefore, a high content of trans -zeatin in the leaves of Pteris vittata may play an important role in its normal growth and tolerance to metals.
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PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2024
·Vol. 35
·期 9
·1-11
·
IF 2.8
Yang Ting,Deng Haoyun,Zhang Zhengli,Hu Mi,Cheng Kaiqiang,Chen Yuanjian,Li Junli
Within organic solar cells (OSCs), the cathode interface layer (CIL) is indispensable for promoting the transport and extraction of charges. In this research, an environmentally friendly and cost-effective trans-cinnamic acid (TCA) is doped into PFN-Br to serve as the CIL in OSCs. The molecular structure and electronic structure of TCA were studied in this article using density functional theory (DFT) calculations executed with the Gaussian’16W software. The calculation results based on DFT indicate that TCA exhibits excellent planarity and the lowest unoccupied molecular orbital (LUMO) matching with PFN-Br. Excellent planarity and matched energy levels are advantageous for charge transport and harvesting. Doping TCA into the PFN-Br cathode interface layer improved charge extraction efficiency, and reduced charge recombination phenomena. Consequently, this led to a notable amplification in the maximum power conversion efficiency (PCE) of PBDB-T: IT-M -based non-fullerene devices, achieving an impressive 11.32%. This study furnishes an uncomplicated and cost-effective strategy for improving the PCE in OSCs.
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FUEL PROCESSING TECHNOLOGY
·
2016
·
IF 3.847
Hu Pan,Hu Li,Xiao-Fang Liu,Heng Zhang,Kai-Li Yang,Shan Huang,Song Yang
Due to the high content of free fatty acids (FFAs), crude Jatropha curcas oil (CJCO) usually requires a two-step process for biodiesel production . In this study, the mesoporous polymeric solid acid consisting of acidic functional ionic liquids and sulfonic acid groups have been synthesized and characterized by XPS , FT-IR, N 2 adsorption-desorption, HR-TEM, SEM and TG. The polymeric solid acid possesses abundant mesopores (27.0 nm), high surface area (281.0 m 2 /g) and high acidity (2.6 mmol g − 1 ). It shows excellent catalytic performance in esterification of oleic acid with methanol, exhibiting 98% oleic acid conversion, which is more active than commercial resins (i.e., Amberlyst 15 and Nafion NR50). Interestingly, it is also efficient for the simultaneous (trans)esterification of CJCO with a high acid value (15.9 mg KOH/g) to biodiesel without soap formation, giving high FAMEs content of 94%. The obtained catalytic activity was demonstrated to be higher than that of conventional acidic catalysts. Moreover, the polymeric solid acid could keep relatively high catalytic activity after four recycles. Therefore, the mesoporous polymeric solid acid is a promising solid acid catalyst for direct production of biodiesel from renewable and low cost feedstocks with high acid values.
研究领域:
能源化学
·
催化材料科学
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高影响因子
RSC Advances
·
2015
·Vol. 5
·期 66
·53926-53934
·
IF 3.84
Yu Zhou,Guofei Chen,Huiwen Zhao,Liping Song,Xingzhong Fang
A series of transparent polyimides was prepared from trans-1,4-bis(2,3-dicarboxyphenoxy)cyclohexane dianhydride (trans-3,3′-CHDPA) with various aromatic diamines via one-step solution polycondensation. The glass transition temperatures (Tgs) of the resulting polyimides were in the range of 206–255 °C. These polyimide films showed high optical transparency with cut-off wavelengths of 370–379 nm, and they exhibited good mechanical properties with tensile strengths of 65–88 MPa, tensile moduli of 1.7–2.4 GPa, and elongations at break of 4.7–7.5%. Compared with polyimides based on trans-1,4-bis(3,4-dicarboxyphenoxy)cyclohexane dianhydride (trans-4,4′-CHDPA), the polyimides derived from trans-3,3′-CHDPA showed higher Tg, better solubility and optical transparency due to the 3- and 4-position isomeric effect.
研究领域:
材料科学
·
高分子化学
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DOI
高影响因子
COMPOSITES PART B-ENGINEERING
·
2025
·
IF 14.2
Xiangren Bai,Xiang Zhang,Dongdong Zhao,Xudong Rong,Naiqin Zhao,Chunnian He
Strain localization at grain boundaries (GBs) is one of the key factors contributing to the trade-off between strength and ductility in engineering alloys. To address this issue, we developed a unique perpendicular interlocking structure connecting the matrix GBs by introducing trans -granular graphene nano-shells (SGSs) via a two-step targeted localized Cu–Al interdiffusion process. In the as-built structure, the original GBs served as the soft component, while the perpendicularly intersecting SGSs acted as the hard component. This configuration not only promotes sustainable dislocation accumulation in the vicinity of the GBs but also facilitates plastic strain delocalization through stress shielding effects from the SGSs. By effectively impeding the extension of shear bands and redirecting crack propagation towards the grain interiors, these hetero-structured GBs contribute to reducing the intrinsic brittleness of GBs. Consequently, the SGS/AlCu composite demonstrates a remarkable balance of tensile strength (∼574 MPa) and ductility (∼17 %). Our findings suggest that restructuring the geometry of GBs in metallic alloys presents a promising strategy for mitigating strain localization at GBs, offering potential for enhancing the properties of metallic alloys in various engineering applications.
研究领域:
材料科学
·
力学工程
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POLYMER JOURNAL
·
2026
·
IF 3.1
Chen Meijia,Song Li,Xiang Hengxue,Zhu Liping,Feng Xunda,Yu Senlong,Zhu Meifang
The development of intrinsically photostable polymers through molecular design remains a persistent challenge in materials science. Here, a series of polyethylene terephthalate-1,4-cyclohexanedimethanol (PETG) copolyesters with precisely tailored trans-1,4-cyclohexanedimethanol (trans-CHDM) contents were synthesized via one-pot copolymerization. The successful stereochemical control of CHDM units was verified by 1 H-NMR spectroscopy. For the first time, the structure-property relationship between the trans-CHDM content and the UV-aging resistance of PETG was systematically established. Increasing the trans-CHDM content was shown to increase the glass transition temperature and the thermal stability of the copolyesters. Remarkably, higher trans-CHDM levels led to significantly improved photostability, as evidenced by superior mechanical retention, reduced chain scission, and minimal structural degradation during prolonged UV-irradiation. Mechanistic investigations revealed that the increased UV resistance stems from a narrowed optical bandgap and, more critically, an efficient photothermal conversion pathway. The latter is facilitated by heightened intramolecular friction in high-trans polymers, which promotes nonradiative energy dissipation and effectively mitigates photooxidative damage. Within the series, PETG-40-80% achieves an optimal balance between UV durability and mechanical performance, positioning it as a promising candidate for high-performance, weather-resistant applications. This work highlights the stereochemical control of comonomers as a potent strategy for increasing the photostability of polyester materials.
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高影响因子
Frontiers in Microbiology
·
2026
·
IF 5.8
Yuling Dong,Beibei Du,Changjian Xie,Shujing Zhang,Qiuxiang Pang,Desheng Zhang
Potassium sorbate (PS) is a widely used antimicrobial additive employed as a preservative in food, cosmetics, and animal feed. Early childhood represents a critical developmental window characterized by rapid growth, immune system maturation, gut microbiota establishment, and physiological homeostasis development, which may be particularly vulnerable to chemical exposures (during which chemical exposures may exert heightened impacts). Nevertheless, the effects of PS on childhood development and gut microbiota remain poorly understood, and its potential trans-generational effects have yet to be elucidated. In this study, we employed Drosophila melanogaster, an established model for studying evolutionarily conserved aspects of development, metabolism, and host–microbiota interactions, to systematically evaluate PS toxicity across multiple parameters: developmental dynamics, gut microbiota composition, gene regulation in adulthood, and trans-generational effects. Our findings demonstrate a dose-dependent biphasic response: while low-dose PS exposure (25 mg/L) accelerated larval pupation and adult emergence, suggesting a potential growth-promoting effect, high-dose exposure (≥ 500 mg/L) significantly delayed development and reduced adult lifespan (observed in females at 1,000 and males at 500–1,000 mg/L). Notably, trans-generational analysis revealed persistent developmental delays in the F1 generation, with offspring of 1,000 mg/L-exposed parents showing prolonged larval pupation time despite normal adult emergence timelines, indicative of partial developmental recovery. Mechanistically, high-dose PS disrupted endocrine signaling and altered expression of key developmental pathway regulators (EcR, InR, TOR, and E74B). These transcriptional changes were largely reversible in offspring, further supporting a similar gradually wash out recovery. While gut microbiota remained stable in exposed parents, offspring of 1,000 mg/L-exposed flies had significant microbiome alterations, highlighting clear trans-generational dysbiosis. This study provides new evidence that PS exposure during a sensitive developmental period in D. melanogaster can perturb development and gut microbial homeostasis across generations, offering insights relevant to understanding how early-life chemical exposures might influence conserved biological processes in higher organisms.
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高影响因子
Nature Communications
·
2026
·
IF 18.1
Cui Jiawen,Xu Nuo,Cao Rongyu,Zhang Ruqi,Yu Ying,Ao Luyao,Wang Feiyi,Gong Qixing,Zhen Le,Li Yantong,Zhou Qingqing,Dang Yue,Wang Guangji,Zhou Fang,Liu Jiali
Trans-endothelial migration (TEM) is a critical rate-limiting barrier in tumor metastasis, yet its mechanistic basis remains poorly defined. Here we show that α-ketoglutarate (α-KG) and its metabolic enzyme glutamate dehydrogenase 1 (GLUD1) constitute a pivotal regulatory axis governing this process. Mechanistically, GLUD1 translocates to the nucleus, where α-KG binds to its non-catalytic site (K147) to induce a conformational change that stabilizes the GLUD1-SNAIL interaction, promoting assembly of a nuclear ternary complex. Notably, this complex is preferentially enriched in tumors, and specific blockade of K147 disrupts this assembly and abrogates metastatic effects. Our findings reveal a non-canonical role for GLUD1 and α-KG in transcriptional regulation and propose a therapeutic paradigm that selectively interferes with metabolite-dependent complex formation rather than enzymatic activity, offering a precise strategy to target TEM-driven metastatic cells.
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