SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2021
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IF 4.098
Huixue Wang,Jinya Liu,Wei Chen,Jintong Na,Yong Huang,Guiyin Li
Glypican-3 (GPC3), a heparin sulfate proteoglycan, is a potential diagnostic and therapeutic target for hepatocellular carcinoma. In this paper, a novel fluorescent aptasensor for GPC3 detection is constructed via [email protected] quantum dots–labeled GPC3 aptamer ( [email protected] Apt ) as a fluorescence probe. First, [email protected] is screened out with higher fluorescence intensity, which emits bright blue fluorescence under ultraviolet light. Then, the fluorescence-labeled [email protected] Apt probe is formed by the combination of amination GPC3 Apt and [email protected] using EDC/NHS coupled reaction. Under hydrogen bond and π-π interaction/stacking, the fluorescence of [email protected] Apt could be quenched by reductive graphene oxide (RGO) with the help of the photoinduced electron transfer and the fluorescence resonance energy transfer mechanism. In the presence of GPC3, the [email protected] Apt specifically recognizes and binds to GPC3, giving rise to the change of secondary structure of GPC3 Apt to form the GPC3/GPC3 Apt [email protected] complex, which would lead to the disintegration of the [email protected] Apt -RGO compound. Therefore, the energy transfer process is blocked and the fluorescence intensity is restored, enabling a highly sensitive response to GPC3. When the concentration of GPC3 is from 5.0 ng/mL to 150.0 ng/mL, the fluorescence recovery rate is well linearly related to GPC3 concentration with the limit of detection of 2.395 ng/mL (S/N = 3). This strategy shows recoveries from 98.31% to 101.89% in human serum samples and provides simple, fast and cheap analysis of GPC3, which suggests that it has great potential applications in clinical diagnosis for hepatocellular carcinoma.
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COMPOSITES PART B-ENGINEERING
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2021
·
IF 9.078
Zhen Lu,Dekai Liu,Chengcheng Shi,Han Xiao,Wei Liu,Shaosong Jiang
NiAl intermetallic compounds have attracted the attention of researchers in the field of high-temperature structural materials, but their poor room-temperature ductility and insufficient high-temperature strength crying out for solutions have hindered their practical application. To solve these problems, a novel 3-dimensional network structure-reinforced NiAl-based composite was designed by hot presing sintering sinter with pre-alloyed powders. The 3-dimensional network structure was composed of discrete nearly spherical HfO 2 and rod-shaped HfRe 2 . The mechanical properties of the composites were closely correlated with the distribution of the 3-dimensional network structure in the matrix, which was controlled by the sintering temperature and holding time. A microstructure with a desirable 3-dimensional network structure distribution and the best comprehensive mechanical properties was produced at 1375 °C/60 min. The microstructural evolution along with the strengthening and toughening mechanisms were discussed in detail. The increase in the high-temperature strength was mainly attributed to the pinning effect of the 3-dimensional network structure on dislocations and the restraining effect on the sliding and rotation between the matrix grains, while the increase in room-temperature ductility was mainly due to the grain refinement and hindering of crack propagation by the 3-dimensional network structure. In this paper, the development of a novel low-density NiAl-based composite with a 3-dimensional network structure improved both the strength and ductility, providing design ideas for the application of NiAl-based materials.
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Molecular Catalysis
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2021
·
IF 5.062
Kequan Chen,Chaoqiang Wu,Chengyong Wang,Alei Zhang,Fei Cao,Pingkai Ouyang
In this study, efficient production of the platform chemical 3-acetamido-5-acetylfuran (3A5AF) from chitin resources via a chemo-enzymatic protocol was investigated. Initially, chitinolytic enzyme was used to convert chitin into N -acetyl glucosamine (GlcNAc) using an affinity adsorption–enzymatic reaction integrated approach. Then, the obtained GlcNAc was used to produce 3A5AF using screened ammonium thiocyanate (NH 4 SCN) as the catalyst. The optimal conditions for converting GlcNAc into 3A5AF were determined at a temperature of 180 °C, solvent of DMAc, a molar ratio of GlcNAc to NH 4 SCN of 2:1, addition of CaCl 2 ·2H 2 O, a molar ratio of GlcNAc to CaCl 2 ·2H 2 O of 1:1 and a reaction time of 10 min, resulting in 3A5AF with a molar yield of 56.66%. The NH 4 SCN showed good recyclability within five cycles. Furthermore, upon scaling up of the 3A5AF preparation, a good yield was still maintained. Additionally, 99% pure 3A5AF needle-shaped crystals were obtained with 34 wt% yield by crystallisation purification. This new chemo-enzymatic protocol provides a good option to convert chitin resources into 3A5AF.
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EXPERIMENTAL CELL RESEARCH
·
2021
·
IF 3.905
Lili Tan,Jie Lu,Limin Liu,Lu Li
Atherosclerosis is the underlying contributing factor of cardiovascular disease, which is a process of inflammation and lipid-rich lesion. Macrophage-derived foam cell is a key hallmark of atherosclerosis and connected with various factors of lipid metabolism. Here, we showed that fatty acid binding protein 3 (FABP3) was upregulated in the aorta of ApoE −/- mice with high-fat-diet (HFD) feeding. Knockdown of FABP3 in HFD-fed ApoE −/- mice notably facilitated cholesterol efflux, inhibited macrophage foam cell formation, and thus prevented atherogenesis. Furthermore, FABP3 silencing decreased the expression of peroxisome proliferator-activated receptor γ (PPARγ). Mechanistic studies had disclosed the involvement of PPARγ signaling in balancing cholesterol uptake and efflux and diminishing foam cell formation. These findings firstly revealed an anti-atherogenic role of FABP3 silencing in preventing foamy macrophage formation partly through PPARγ, which might be a beneficial approach for therapying atherosclerosis.
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2021
·
IF 7.312
Zhongwei Guo,Kaifei Zhang,Hui Guan,Meihong Liu,Sanchuan Yu,Congjie Gao
Surface modification has been adopted to tune surface property of nanofiltration membrane for improved separation performance. However, it has rarely been explored to modulate membrane pore size distribution. Here, we reported a novel modification strategy combined with surface cross-linking and in-situ condensation for fabricating composite nanofiltration membrane with reduced and homogenized pore size. The strategy was fulfilled by soaking the surface of nascent polyamide-based membrane with an aqueous solution of 3-aminopropyltriethoxysilane (APTES). Membrane physico-chemical property analyses demonstrated that the hydrolyzed APTES molecules bonded onto membrane surface through amidation and esterification reactions with the residual acyl chloride groups, and chemically combined with each other through condensation reaction. Modification was found to hydrophilize membrane surface, homogenize pore size and reduce mean pore diameter. The modification under desired conditions led to an improvement in pure water permeance from 12.2 to 14.0 l/m 2 h bar, a decrease in geometric mean pore diameter from 0.71 nm to 0.63 nm, and a decline in geometric standard deviation from 1.32 to 1.23. Permeation test results showed that the APTES-modified membrane also exhibited improved removal performance to methylene blue and cresol red removal and enhanced antifouling resistances to model foulants cetyltrimethylammonium bromide, humic acid and bovine serum albumin.
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JOURNAL OF THE EUROPEAN CERAMIC SOCIETY
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2021
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IF 5.302
Lang Bian,Xudong Qi,Kai Li,Jinhui Fan,Zhanmiao Li,Enwei Sun,Bin Yang,Shuxiang Dong,Wenwu Cao
Ternary 0.552Pb(Ni 1/3 Nb 2/3 )O 3 -xPbZrO 3 -(0.448-x)PbTiO 3 (PNN-PZ-PT) ceramics near the triple point compositions were fabricated by an improved two-step sintering method. The triple point composition 0.552PNN-0.135PZ-0.313PT ceramic has outstanding piezoelectric performance with piezoelectric coefficient d 33 = 1200 pC/N. Its easy fabrication and low cost make this piezoelectric material an excellent candidate for high sensitivity sensors and ultrasonic transducers. The evolution of domain structures for ceramics with composition near the triple point provides deeper insight into the mechanism of ultrahigh piezoelectric properties of PNN-PZ-PT ceramics.
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MEDICINAL CHEMISTRY RESEARCH
·
2021
·Vol. 30
·期 6
·1240-1248
·
IF 1.965
Shi Bo-Ya,Wang Ze-Hao,Zhang Ning,Xie Yin-Rong,Sun Xiu-Li,Yang Hao-Nan,Wu Yi-Long,Zi Cheng-Ting,Wang Xuan-Jun,Sheng Jun
Novel glucosylated (-)-epigallocatechin-3-gallate derivatives 10 – 13 having the EGCG analogs conjugated to the d -glucosyl azide were synthesized by carrying out the copper-catalyzed azide-alkyne cycloaddition (CuAAC) reaction, and were evaluated for their cytotoxicities against a panel of five human cancer cell lines (HL-60, SMMC-7721, A-549, MCF-7, and SW480) using MTT assays. Compounds 10 and 11 showed the highest levels of cytotoxicity against the HL-60 cells with IC 50 values of 4.57 and 3.78 μM, respectively, and showed moderate selectivity toward cancer cell lines. Compound 11 was also shown to induce apoptosis in HL-60 cells. Most notably, inclusion of the perbutyrylated glucose residue in an EGCG derivative was concluded to lead to increased anticancer activity.
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BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
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2021
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IF 3.575
Likun Yan,Xiao Zhang,Jie Fu,Qiang Liu,Xiaohua Lei,Zhenyu Cao,Ju Zhang,Yaoli Shao,Qing Tong,Wei Qin,Xinxu Liu,Chun Liu,Zhiqiang Liu,Zhenghao Li,Jueliang Lu,Xundi Xu
Transient receptor potential vanilloid 3 (TRPV3) is a member of the TRP superfamily. Previous studies have demonstrated that TRPV3 is associated with myocardial fibrosis. However, the role of TRPV3 in hepatic fibrosis and its underlying mechanisms are still unclear. This study aimed to elucidate the underlying effects of TRPV3 on hepatic fibrosis at multiple biological levels. First, immunohistochemical staining was performed to examine TRPV3 expression in human hepatic cirrhosis tissues. Then, we established a CCl 4 -induced hepatic fibrosis mouse model. The TRPV3 selective agonist drofenine and its inhibitor, forsythoside B, were intraperitoneally injected to investigate the relationship between TRPV3 and liver fibrosis progression. Finally, in vitro studies were performed using hepatic stellate cells (HSCs) to discover the potential molecular biological mechanisms. Immunohistochemistry revealed TRPV3 overexpression in liver cirrhosis. In the liver fibrosis groups, TRPV3 inhibitor treatment significantly reduced liver fibrosis, while TRPV3 agonist exacerbated its progression. In HSCs, knocking down TRPV3 with siRNA impaired DNA synthesis and cell proliferation and increased cell apoptosis. Furthermore, we found that knockdown of TRPV3 could reduce the lectin like oxidized lowdensity lipoprotein receptor-1 (LOX-1) protein levels. Our research suggests that lower expression or functional levels of TRPV3 can ameliorate the inflammatory response and fibrotic tissue proliferation.
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Journal of Advanced Ceramics
·
2021
·Vol. 10
·期 4
·809-819
·
IF 6.707
Yan Tianxiang,Chen Kaiyuan,Li Chengqi,Liu Min,Wang Jie,Fang Liang,Liu Laijun
(1− x )K 0.5 Na 0.5 NbO 3 − x Bi(Zn 2/3 Nb 1/3 )O 3 ((1− x )KNN− x BZN, x = 0.010, 0.015, 0.020, 0.025, and 0.030) lead-free ceramics were fabricated via a traditional solid-state method. The crystal structure, microstructure, dielectric, and conductivity behavior of this system were studied. Combined with X-ray diffraction (XRD) patterns, Rietveld refinement, and dielectric spectroscopy, an orthorhombic phase was determined for x = 0.010, an orthorhombic-tetragonal mixed phase was identified for x = 0.015, and a rhombohedral symmetry appears in 0.020 ⩽ x ⩽ 0.030. Both 0.98KNN−0.02BZN and 0.975KNN−0.025BZN ceramics exhibit stable permittivity and low dielectric loss tangent (tan δ ) in wide temperature ranges owing to the combination of rhombohedral-tetragonal step-like feature and the diffuse phase transition from tetragonal to cubic. The activation energies of dielectric relaxation and conductivity behavior at high temperatures initially decrease slightly, then drop sharply, and finally decline slowly, which could be attributed to microstructure morphologies and the concentration of oxygen vacancies.
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ELECTROCHIMICA ACTA
·
2020
·
IF 6.215
Sixuan Xiao,Yujie Zhang,Debao Xiao
Visualization of latent fingermarks has ever been realized through electrochromism (EC) on stainless steel used as an electrode of electrochromic device (ECD). However, enhancement of fingermarks on paper using EC remains unresolved. Considering the hydrophobicity differences between the protruding and the concave parts of sebum-containing fingermarks, we attempt to devise ECDs capable of visualizing latent fingermarks on copperplate paper. It is demonstrated that the sulfonated viologen, 1,1′-bis(3-sulfonatopropyl) viologen can be used as an excellent chromophore for latent fingermarks enhancement on copperplate paper using EC, with either 1,1′-ferrocenedimethanol, or vitamin C or potassium ferrocyanide/potassium ferricyanide as the electron mediator. The as-fabricated ECDs can display the characteristic second level details of latent fingermarks left on copperplate paper under a voltage of -1.2 V. This work opens a new avenue for visualizing latent fingermarks on copperplate paper, which is a frequently-used potential physical evidence in office and daily life that may be useful for forensics.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2020
·
IF 4.192
Jiaojiao Li,Li Zhang,Tao Wu,Yafei Li,Xiaojun Zhou,Zheng Ruan
Destruction in intestinal barrier is concomitant with the intestinal diseases. There is growing evidence that tryptophan-derived intestinal bacterial metabolites play a critical role in maintaining the balance of intestinal mucosa. In this study, the Caco-2/HT29 coculture model was used to evaluate the effect of indole-3-propionic acid (IPA) on the intestinal barrier and explore its underlying mechanism. We found that IPA increased transepithelial electrical resistance and decreased paracellular permeability which was consistent with the increase in tight junction proteins (claudin-1, occludin, and ZO-1). Furthermore, IPA strengthened the mucus barrier by increasing mucins (MUC2 and MUC4) and goblet cell secretion products (TFF3 and RELMβ). Additionally, IPA weakened the expression of LPS-induced inflammatory factors. These discoveries provide new views for understanding the improvement of intestinal barrier by gut microbial metabolites of aromatic amino acids.
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Natural Product Communications
·
2020
·
IF 0.468
Na Li,Dongdong Xin,Hongbo Li,Yanyan Zhao,Wei Zhou,Hao Zhang,Liangbin Hu,Yaming Ge,Haizhen Mo
Fluoride is an essential trace element, but its beneficial range is narrow, and excess fluoride may have negative health effects. The objective of this study was to investigate the potential cytoprotective effects of epigallocatechin-3-gallate (EGCG) in cultured neuro-2a neuroblastoma cells exposed to sodium fluoride (NaF)-induced oxidative stress. Isolated Neuro-2a cells were exposed to increasing concentrations of NaF (0, 1, 2, 4, 6, and 8 mM) for 24 hours to induce oxidative stress. Moreover, to determine the concentration of EGCG necessary for protective effects, we exposed isolated Neuro-2a cells to increasing concentrations of EGCG (0, 0.5, 1, 5, 10, 20, 40, 60, 80, and 100 μg/mL) for 24 and 48 hours. Pretreatment with EGCG at various doses (0, 0.5, 1, 5, 10, 20, and 40 μg/mL) was evaluated in Neuro-2a cells for 24 hours, followed by an NaF (4 mM per culture well) challenge for 24 hours. As shown in this study, EGCG can protect Neuro-2a cells from NaF-induced apoptosis. This effect may be due to the reactive oxygen species scavenging activity of EGCC.
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