Molecular Cancer
·
2023
·Vol. 22
·期 1
·1-20
·
IF 37.3
Song Runjie,Ma Shuoqian,Xu Jiajia,Ren Xin,Guo Peilan,Liu Huijiao,Li Peng,Yin Fan,Liu Mei,Wang Qiang,Yu Lei,Liu Jiali,Duan Binwei,Rahman Nafis A.,Wołczyński Sławomir,Li Guangming,Li Xiangdong
Background hsa_circ_0001727 (circZKSCAN1) has been reported to be a tumor-associated circRNA by sponging microRNAs. Intriguingly, we found that circZKSCAN1 encoded a secretory peptide (circZKSaa) in the liver. The present study aims to elucidate the potential role and molecular mechanism of circZKSaa in the regulation of hepatocellular carcinoma (HCC) progression.Methods The circRNA profiling datasets (RNA-seq data GSE143233 and GSE140202) were reanalyzed and circZKSCAN1 was selected for further study. Mass spectrometry, polysome fractionation assay, dual-luciferase reporter, and a series of experiments showed that circZKSCAN1 encodes circZKSaa. Cell proliferation, apoptosis, and tumorigenesis in nude mice were examined to investigate the functions of circZKSaa. Mechanistically, the relationship between the circZKSaa and mTOR in HCC was verified by immunoprecipitation analyses, mass spectrometry, and immunofluorescence staining analyses.Results Receiver operating characteristic (ROC) analysis demonstrated that the secretory peptide circZKSaa encoded by circZKSCAN1 might be the potential biomarker for HCC tissues. Through a series of experiments, we found that circZKSaa inhibited HCC progression and sensitize HCC cells to sorafenib. Mechanistically, we found that the sponge function of circZKSCAN1 to microRNA is weak in HCC, while overexpression of circZKSaa promoted the interaction of FBXW7 with the mammalian target of rapamycin (mTOR) to promote the ubiquitination of mTOR, thereby inhibiting the PI3K/AKT/mTOR pathway. Furthermore, we found that the high expression of cicZKSCAN1 in sorafenib-treated HCC cells was regulated by QKI-5.Conclusion sThese results reveal that a novel circZKSCAN1-encoded peptide acts as a tumor suppressor on PI3K/AKT/mTOR pathway, and sensitizes HCC cells to sorafenib via ubiquitination of mTOR. These findings demonstrated that circZKSaa has the potential to serve as a therapeutic target and biomarker for HCC treatment.
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JOURNAL OF MATERIALS SCIENCE
·
2022
·Vol. 57
·期 43
·20531-20542
·
IF 4.682
Gao Yuan,Qi Yanfei,Wang Shuaishuai,Zhou Xinghai,Lyu Lihua,Jin Guoqing
The interface structure greatly affects the material surface wettability. Polyphenylene sulfide (PPS) fiber paper was modified via regulation of thermally induced phase separation process to obtain interfacial nanofiber network-like morphology, which obeyed nucleate-growth mechanism with the PPS resin on the fiber surface as the core. PPS nanofiber network layer with rough concave surface feature endowed the modified PPS fiber paper with stable superhydrophobic properties, and it showed excellent oil absorption and oil/water separation performance. The separation efficiency of using P -2 paper to separate the five oil/water mixtures listed in this paper were more than 99.99%, and the kerosene flux was more than 7350 L m −2 h −1 driven by gravity. Excellent hydrophobicity and cycling performance made PPS fiber paper with excellent reusability. In addition, the PPS fiber paper had also been proved to be used in strong acid–base and organic solvents for a long time. This work provided a PPS fiber paper for oil/water separation with simple preparation process, which was expected to be produced in large scale and applied in industrial field. Graphical abstract
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MICROCHIMICA ACTA
·
2022
·Vol. 190
·期 1
·1-9
·
IF 6.408
Wang Jiarui,Feng Juanjuan,Sun Min,Lian Yujing,Wang Minglin,Qiao Luqin
Sulfonic acid-functionalized covalent organic frameworks (COF-SO 3 ) as a coating of stir bar sorptive extraction (SBSE) for capturing three fluoroquinolones from milk have been developed. The COF-SO 3 material was characterized by X-ray diffraction, scanning electron microscopy, X-ray photoelectron spectroscopy, and infrared spectroscopy. Milk without any typical treatments like protein precipitation and defatting was only diluted five times with water for test. Combined with high-performance liquid chromatography (HPLC), a SBSE-HPLC method was established for detecting fluoroquinolones in milk samples. The corresponding wide linear ranges (4.00–500.0 µg L −1 ), low detection limits (1.20–2.62 µg L −1 ), good test repeatability (RSD < 5.2%), and acceptable enrichment factors (56.2–61.5) were implemented for three fluoroquinolones. The analytical method was applied to determine trace targets and provided satisfactory results. Furthermore, the research displayed satisfied reproducibility for bar-to-bar (RSD < 6.5%) and batch-to-batch (RSD < 8.6%) tests. Graphical abstract
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JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY
·
2022
·Vol. 148
·期 3
·789-805
·
IF 4.755
Zhu Di,Huang Zhixiong,Shi Minxian,Qin Yan,Zou Zhenyue,Deng Zongyi
BPR has been widely used as ablative material for thermal protection on aircraft surface. In this study, model-free method was employed to study the kinetic models of BPR and ZrSi 2 /B 4 C-Ph composite. In addition, the thermal stability, chemical reaction and structure evolution during pyrolysis of ZrSi 2 /B 4 C-Ph composite were characterized by TGA, XRD, XPS, GC–MS and TG-MS. The results indicated that the pyrolysis of BPR includes three consecutive and overlapping stages. And the kinetic mechanism function f(α) , apparent activation energy E a and pre-exponential factor A of each stage were confirmed. Besides, the high char yield of ZrSi 2 /B 4 C-Ph composite is mainly attributed to the reaction of ZrSi 2 and B 4 C with gases released during BPR pyrolysis, which formed solid phases such as amorphous carbon, ZrO 2 , SiO 2 and B 2 O 3 .
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FIBERS AND POLYMERS
·
2022
·Vol. 23
·期 11
·3022-3027
·
IF 2.347
Yu Miao,Chen Zhuoming,Xin Binjie,Liu Xunlin,Xu Wenjun
Pearl is a pure natural material, which has multifunctional properties such as anti-ultraviolet, anti-static and far-infrared physiotherapy. In this study, a series of polyacrylonitrile/pearl nanofibrous membranes (PPFM) with different pearl powders were prepared by the electrospinning method. The membranes were then investigated by scanning electron microscopy (SEM) and fourier-transform infrared spectroscopy (FTIR), and their functional properties such as far-infrared emission, ultraviolet (UV) protection, and electrical resistivity were also studied. The experimental results reveal that pearl powders can significantly enhance the far-infrared emission of polyacrylonitrile (PAN), and the average emissivity was increased up to 0.795 in the wavelength range of 8–14 μm when the pearl powder mass fraction increased to 9 %. In addition, the UV protection factor (UPF) of the membrane with 9 % pearl powder could reach 968, while the UPF of the pristine PAN membrane was only 11, and the electrical resistivity of PPFM was also improved after blending with pearl powders. The study proves that pearl powder can efficiently endow the PAN with multifunctional properties for more promising applications.
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Nature Communications
·
2022
·Vol. 13
·期 1
·1-13
·
IF 17.694
Sun Jie,Wang Ming,Zhong Yaqi,Ma Xuan,Sun Shimin,Xu Chenzhong,Peng Linyuan,Li Guo,Zhang Liting,Liu Zuojun,Ai Ding,Liu Baohua
The progressive decline of physiological function and the increased risk of age-related diseases challenge healthy aging. Multiple anti-aging manipulations, such as senolytics, have proven beneficial for health; however, the biomarkers that label in vivo senescence at systemic levels are lacking, thus hindering anti-aging applications. In this study, we generate a Glb1 +/m ‒Glb1-2A-mCherry (GAC) reporter allele at the Glb1 gene locus, which encodes lysosomal β-galactosidase—an enzyme elevated in tissues of old mice. A linear correlation between GAC signal and chronological age is established in a cohort of middle-aged (9 to 13 months) Glb1 +/m mice. The high GAC signal is closely associated with cardiac hypertrophy and a shortened lifespan. Moreover, the GAC signal is exponentially increased in pathological senescence induced by bleomycin in the lung. Senolytic dasatinib and quercetin (D + Q) reduce GAC signal in bleomycin treated mice. Thus, the Glb1-2A-mCherry reporter mice monitors systemic aging and function decline, predicts lifespan, and may facilitate the understanding of aging mechanisms and help in the development of anti-aging interventions.
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Food and Bioprocess Technology
·
2022
·Vol. 16
·期 1
·232-246
·
IF 5.581
Deng Yejun,Liu Yong,Zhang Caihong,Xie Pujun,Huang Lixin
The accumulation of advanced glycation end-products (AGEs) from food matrix in human body can induce health crisis. This study firstly investigated the AGEs accumulation reducing potential of soluble dietary fiber (SDF) from Rhodomyrtus tomentosa fruits in antiglycation and trapping AGEs aspects. To obtain superior functionalities, the SDF was prepared by an enzymatic modified extraction method (ESDF). The result showed the extraction yield of SDF increased by 45% with the Viscozyme L modification. The structural characterizations indicated the enzymatic treatment greatly influenced the monosaccharide composition, thermal stability, crystallinity, and apparent viscosity of Rhodomyrtus tomentosa fruits SDF (RTSDF) as compared with SDF obtained from water extraction method (WSDF). The antioxidant assay indicated that RTSDF could scavenge O 2−∙ ,OH ∙ , and H 2 O 2 radicals and chelate Fe 2+ . Additionally, RTSDF, especially ESDF, reduced the more fluorescent AGEs generation in bovine serum albumin (BSA)-fructose model, and protected the BSA from oxidation and cross-linking. Besides, the RTSDF presented the ability to trap those already formed BSA-bound AGEs. ESDF exhibited higher AGEs binding capacity, while WSDF demonstrated stronger ability in restraining the AGEs diffusion. These results suggested the potential utility of RTSDF serving as an essential ingredient in functional foods to reduce AGEs accumulation in human body, controlling AGEs-associated diseases.
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MICROCHIMICA ACTA
·
2022
·Vol. 189
·期 12
·1-10
·
IF 6.408
Liu Xiao-Pan,Sun Wen-Qian,Zhao Meng-Ge,Zhang Xing-Jie,Liu Li-Hong,Chen Chang-Po
An easily prepared fluoro-functionalized ionic covalent organic framework (F-iCOF) has been implemented into MALDI-TOF MS, enabling the highly selective enrichment and sensitive determination of perfluorinated sulfonate (PFS) contaminants in a rapid and convenient manner. The good thermal stability and excellent optical absorption properties of F-iCOF makes it a brilliant matrix with no background noise. Moreover, benefitting from the large surface area, appropriate pore size, good water dispersibility, and abundant fluorine atom and cationic characteristic of F-iCOF, it exhibited superior adsorption capacity and enrichment selectivity towards PFSs. Good signal responses for PFSs were obtained in the presence of various interfering compounds such as BSA, HA, or even more than 100-fold excess of glutamic acid and similar in structure sodium alkyl sulfonates, highlighting the specific selectivity of F-iCOF. Calibration curves for potassium perflurobutane sulfonate (PFBSK) in tap water and whole blood were established with good linear correlation in the range 1–500 pg mL −1 . The limits of detection and quantification for PFBSK were as low as 0.04 pg mL −1 and 0.05 pg mL −1 , respectively, which are comparable or better than the existing methods for the determination of PFSs. Graphical Abstract
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ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2022
·Vol. 30
·期 9
·24470-24478
·
IF 5.19
Ma Ge,Xiao Han,Wei Xinming,Xiao Anshan,Sun Xiaoying,Gao Xiang
Oil leakage from water coolers in refinery circulating water occurs from time to time, which affects the long-term and stable operation of refinery units. So far, workers in the refineries still adopt manual check methods, opening water coolers one by one and checking the water’s smell and color to find out the spilled water coolers. In this study, a more rapid method of source appointment of oil spill in the circulating water by combining chemical fingerprinting with model recognition was developed. Firstly, chemical fingerprints including benzene/naphthalene series, and light hydrocarbon (C 3 -C 5 ) in oil samples from all water coolers in the refinery fluid catalytic cracking (FCC) unit were analyzed by gas chromatography-mass spectrometry (GC/MS). Gasoline, diesel, and poor oil could be distinguished in terms of benzene and naphthalene distribution. The three similar types of gasolines could be distinguished by the volatile hydrocarbons especially C 3 -C 4 . The classification model for the spill of gasoline, diesel, and poor oil in circulating water was constructed by the partial least squares discriminant analysis algorithm with a 100% correct classification rate at the concentration more than 10 ppm. The gasoline spills in the circulating backwater of the refinery were successfully recognized by the classification model. This method enables the rapid prediction of oil spill type in refinery circulating water, and a similar method by installing online instrument and software potentially can be used for monitoring the circulating water in real time.
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FIBERS AND POLYMERS
·
2023
·Vol. 24
·期 1
·299-308
·
IF 2.5
Xu Qingbo,Wang Xinyu,Zhou Jing,Wang Peng,Zhang Yanyan,Chen Qian
Improving the surface roughness of cotton fabric is one of the main strategies to prepare superhydrophobic cotton fabric. To improve the surface roughness of fabric, it is advisable to immobilize inorganic/organic particles on the surface of cotton fabric. However, the durability of the superhydrophobic cotton fabric is very poor due to the inorganic/organic particles are easily detachable from the fabric surface. In this work, a mild eutectic solvent prepared by choline chloride and oxalic acid was used to etch the cotton fabric, making the surface of the cotton fabric have many ravines. These ravines on the cotton fabric can be used the embedded the silica particles, thus making the silica particles firmly immobilized on the cotton fibers. Then, the treated cotton fabric was coated with polydimethylsiloxane (PDMS). As a result, the water contact angle of the finished fabric was reached at 156.9°. In addition, the superhydrophobic cotton fabric constructed by silica particles and PDMS via “Mosaic Mode” showed excellent mechanical and chemical stability. Therefore, the finished fabric has great potential application in the self-cleaning and oil–water separation fields.
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APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
·
2023
·Vol. 195
·期 7
·4552-4569
·
IF 3
Nawaz Muhammad,Jiang Yipeng,Xiao Ying,Yu Hao,Wang Zikang,Hu Kun,Zhang Tianao,Hu Jiajun,Gao Min-Tian
The complex structure of rice straw is such that its bioconversion requires multiple physical and chemical pretreatment steps. In this study, it was found that a large amount of soluble polysaccharides (SPs) are formed during the pretreatment of straw. The yield of NaOH-based SPs (4.8%) was much larger than that of ball-milled SPs (1.5%) and H 2 SO 4 -based SPs (1.1%). For all the pretreatments, the ratio of phenolic compounds to saccharides (P/S) for each type of SPs increased upon increasing the concentration of ethanol in the order of 90% > 70% > 50%. The yield of NaOH-based SPs was much higher than that of acid-based and ball-milled SPs. The changes in the 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS), ferric reducing antioxidant power assay (FRAP), and 2,2-diphenyl-1-picrylhydrazyl (DPPH) of SPs follow the same rule, i.e., the higher the P/S ratio, the higher the antioxidant values of the SPs. The flow cytometry and laser scanning microscopy results show that the P/S ratio can significantly influence the effect of SPs on microbial growth and cell membrane permeability. Upon varying the ethanol concentration in the range of 50–90%, the P/S ratio increased from 0.02 to 0.17, resulting in an increase in the promoting effects of the SPs on yeast cell growth. Furthermore, H 2 O 2 , NAD + /NADH, and NADP + /NADPH assays indicate that SPs with a high P/S ratio can reduce intracellular H 2 O 2 and change the intracellular redox status.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2023
·Vol. 415
·期 7
·1347-1355
·
IF 4.3
Zhang Gan,Zhang Ganggang,Lai Xiaocui,Su Liu,He Weihua,Lai Weihua,Deng Shengliang
Aggregation-induced emission luminogens (AIEgens) are attracting extensive research attention in the biosensor fields. Herein, we report a new polyethyleneimine (PEI)-induced strategy for enhancing luminescence of TCBPE (an AIEgen) to promote its development in biosensor. The copolymer dots (TCBPE-PEI) with high quantum yield (39.7%) and outstanding stability were synthesized via a one-pot method. The fluorescence enhancement mechanism based on the PEI strategy originated from the restriction of intramolecular motions of TCBPEs and the form of donor–acceptor structures to decrease the inherent energy bandgap. Benefiting from chelating property of TCBPE-PEI by Cu 2+ , a fluorescence-quenching sensor for Cu 2+ detection was developed based on the fluorescence quenching of the electron transfer effect. Especially, a good linear range of 10–250 nM with a low limit of detection 1.1 nM was achieved, and it was further applied in samples successfully. The current work provides a novel approach to fabricate AIEgen biosensors and shows great potential in Cu 2+ detection. Graphical Abstract
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危险品化学品经营许可证（带存储）