JOURNAL OF HAZARDOUS MATERIALS
·
2025
·
IF 11.3
Nei Wu,Yuling Yang,Jianming Xu,Haizhen Wang
Heavy-metal-tolerant bacteria provide a sustainable, eco-friendly approach to remediate cadmium (Cd) contaminated soils, and elucidating their tolerance mechanisms is key for designing effective bio-remediation strategies. This study integrated whole-genome sequencing, untargeted metabolomics and multi-phenotypic analyses to reveal the Cd tolerance mechanisms and soil remediation effects of three plant-growth-promoting bacteria: Rhizobium sp. WW22, Burkholderia sp. WW13 and Pseudomonas sp. WW2. Results showed that three strains achieved Cd adsorption efficiencies of 60.27 %-99.56 % in 0.3–100 mg L-1 Cd solutions, with amino, carboxyl, hydroxyl, and amide groups on cell surfaces as major binding sites. They have evolved gene clusters related to metal transport/efflux and oxidative stress response, which synergistically maintain metabolic homeostasis under Cd stress. Among them, strain WW13 exhibited stronger Cd tolerance due to abundant unique gene clusters for antioxidant defense, siderophore synthesis, and putrescine metabolism. Notably, three strains showed convergent metabolic responses under Cd stress, with secretions (predominantly organic oxygen compounds, lipids/lipid-like molecules, benzenoids, and organoheterocyclic compounds) that significantly increased environmental pH. Key common upregulated secretions (itaconic acid, cis/trans-aconitic acid) increased soil pH by 0.01–0.32 units and reduced the proportion of exchangeable Cd by 2.22 %-9.19 % within 10 days. Pot experiments further confirmed that strains indirectly reduced Cd bioavailability by increasing soil pH, enriching hydroxyl/carboxyl/amino groups, regulating dissolved organic carbon/nitrogen, ammonium nitrogen, activities of β-glucosidase and N-acetyl-β- D -glucosaminidase. By day 14, the proportion of exchangeable Cd was reduced by 4.09 %-5.86 %. This study revealed multi-pathway Cd tolerance mechanisms in bacteria and their roles in soil Cd immobilization, providing theoretical basis and efficient strains for bioremediation of Cd-contaminated soils.
研究领域:
环境微生物学
·
土壤生物学
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分子生态学
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重金属污染修复
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环境生物技术
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代谢组学
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植物-微生物互作
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产品关联
DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
·
2018
·
IF 4.439
Qing Shao,Zhen Hu,Xirong Xu,Long Yu,Dayu Zhang,Yudong Huang
The composites with interfacial self-healing ability are smart and promising materials in the future. Although some approaches have been used to heal the micro-cracks in composite materials, it is still a great challenge to develop a versatile strategy to fabricate multifunctional interface for self-healing. Here, boron nitride nanosheets (BN) are immobilized onto PBO fibers by facile polydopamine (PDA) chemistry. Benefiting from the photothermal effect of BN-PDA, the obtained surface layer displays interfacial self-healing properties under Xenon light irradiation.
研究领域:
材料科学
·
化学工程
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DOI
高影响因子
CELLULOSE
·
2021
·Vol. 28
·期 7
·4179-4189
·
IF 5.044
Zhang Shaobo,Zhou Yuhang,Zhu Gaolu,Jiang Yongze,Xie Jiulong,Qi Jinqiu,Yang Hanbo,Hui Wenkai,Huang Jintian
Abstract The magnetic properties of the magnetic transparent composite films largely depend on the content of Fe 3 O 4 employed. However, higher addition of Fe 3 O 4 into the matrix will lead to Fe 3 O 4 nanoparticles agglomeration resulting in the reduction in the transparency of the composite films. The contradiction between Fe 3 O 4 nanoparticle content and transparency limits the application of transparent magnetic films. In this work, the spherical bamboo nanocellulose (SBN) with an average size of 61 nm was prepared from bamboo powder by NaOH/Urea/Thiourea aqueous system, which was an effective, inexpensive and less toxic strategy. The basic structure of SBN was investigated. The SBN exhibited the cellulose II type crystal structure. The molecular chain length of SBN were lower than before dissolution. The magnetic SBN/Fe 3 O 4 composite films with light penetration were prepared. The results revealed that the dispersion of Fe 3 O 4 nanoparticles in the SBN/Fe 3 O 4 composite films was much better than that in nanofibrillated cellulose (NFC)/Fe 3 O 4 composite films because of the abundant hydrogen bonds between Fe 3 O 4 nanoparticles and SBN. The transparent results indicated the highest transmittance of the films was 94% in the wavelength range of visible light, which was almost 23.7% higher than that of NFC/Fe 3 O 4 composite films with the same amount of Fe 3 O 4 addition (15 wt%). The findings in this study also revealed that the SBN in the SBN/Fe 3 O 4 composite films was distributed in layers. The saturation magnetization of the SBN/Fe 3 O 4 composite films was 10.65 emu g −1 with 15 wt% Fe 3 O 4 incorporated, which guaranteed the excellent magnetic properties. Graphic abstract
研究领域:
材料科学
·
纳米技术
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DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2021
·
IF 13.273
Ying Tang,Wenyuan Li,Yaseen Muhammad,Shanliang Jiang,Meiyun Huang,Hanzhuo Zhang,Zhenxia Zhao,Zhongxing Zhao
Hollow covalent organic framework microsphere (H-COF-OMe) using TAPB and DMTP with enriched interfacial defects was fabricated via emulsion interfacial polymerization and in turn applied as a novel host for high laccase loading and tetracycline (TC) degradation. Benefited from the space-confined growth in the multiphase solvent interface, H-COF-OMe exhibited hollow spherical microstructure, high surface area and unique defects-rich interface. Attributed to these intriguing aspects, H-COF-OMe achieved maximum loading capacity of 567 mg/g and activity recovery of 85% for laccase. H-COF-OMe efficiently stabilized the active conformation of laccase from structural distortion via multiple binding sites, which endowed Lac@H-COF-OMe significantly higher pH, thermal, and storage stabilities, and reusability than free laccase and Lac@COF-OMe. Interestingly, the hollow morphology and defective interface of Lac@H-COF-OMe accelerated the diffusion of TC and shortened the reaction pathway, which endowed it with markedly enhanced TC degradation and recycling performance than many state-of-the-art catalysts. Significantly, Lac@H-COF-OMe (20 mg) could achieve 99% degradation of 50 mg/L tetracycline (50 mL) within 100 min. Monitoring of the intermediate products indicated that Lac@H-COF-OMe showed outstanding detoxification performance of the degradation products. This work suggested a novel COF synthesis strategy as laccase immobilization supporters for high TC degradation, which makes it as a promising candidate for degradation of organic pollutants.
研究领域:
环境科学
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生物技术
·
材料科学
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DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2015
·
IF 6.409
Guangxia Yu,Weixiang Wu,Qiang Zhao,Xiaoyun Wei,Qing Lu
This work introduced an efficient immobilization of acetylcholinesterase (AChE) onto amino functionalized carbon nanotubes (CNT–NH 2 ), in order to fabricate high sensitive and practical organophosphorus pesticide (OPs) biosensors. Compared with the pristine, –COOH and –OH decorated CNTs, there were larger amount of enzymes adsorbed on the surface of CNT–NH 2 with a favorable orientation and the best amperometric response was obtained on the AChE/CNT–NH 2 /GC electrode. Furthermore, the biosensor modified with CNT–NH 2 showed a high affinity to acetylthiocholine chloride (ATCh) and could catalyze the hydrolysis of ATCh with an apparent Michaelis–Menten constant ( K m ) value of 67.4 µM. Using paraoxon as a model compound, wide linear ranges from 0.2 nM to 1 nM and 1 nM to 30 nM, and a low detection limit of 0.08 nM were obtained with satisfactory reproducibility and stability. Moreover, the biosensor had also been successfully employed for the determination of low concentrations of pesticides in real vegetable samples. This method could be extended to other functionalized nano–materials for their application in constructing biosensors.
研究领域:
分析化学
·
环境科学
·
生物技术
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DOI
高影响因子
Polymer Chemistry
·
2020
·Vol. 11
·期 35
·5693-5700
·
IF 5.342
Taijun He,Zhenyu Xing,Yixing Wang,Difeng Wu,Yang Liu,Xiangyang Liu
Generally, surface initiated-atom transfer radical polymerization (SI-ATRP) often suffers from the immobilization of the initiator on the surface-inert substrate. Developing an effective and versatile one-step method to anchor the priming sites while retaining the bulk properties of the substrate still faces challenges. In this work, direct fluorination is proposed to treat the benzene ring-containing substrate and the C–F introduced on the benzene ring can be used as an initiation site to achieve SI-ATRP. Computer and molecular simulation experiments have shown that the C–F added on the phenyl ring is more suitable for initiating ATRP than the C–F on the alkane chain. Thereafter, polyethylene terephthalate (PET) fabric was successfully grafted with fluorinated methacrylate polymer via direct fluorination-initiated ATRP, resulting in durable superhydrophobic properties, which demonstrated the practicability of the method. Furthermore, fluorination-initiated ATRP presents universality with other phenyl ring-containing polymeric substrates, such as aramid fiber, polyimide fiber and poly-p-phenylene benzobisoxazole fiber. Among them, the fluorinated aramid fiber can be grafted with hydroxyethyl methacrylate to obtain increased interfacial shear strength.
研究领域:
材料科学
·
高分子化学
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DOI
高影响因子
PLASMA CHEMISTRY AND PLASMA PROCESSING
·
2011
·Vol. 31
·期 6
·811-825
·
IF 1.798
Wei Yanlin,Chen Yashao,Liu Peng,Gao Qiang,Sun Yu,Huang Changzheng
To improve application of hydrophobic polymethyl methacrylate intraocular lens (PMMA IOL) in a convenient and continuous way, 2-hydroxyethyl methacrylate (HEMA) was immobilized by dielectric barrier discharge plasma at relatively high pressure. The hydrophilicity and topography of the modified IOL surface were comprehensively evaluated by contact angle and atomic force microscopy, while the surface biocompatibility of the modified IOL was investigated by platelets adhesion and cells proliferation experiments. The results revealed that the hydrophilicity of the HEMA- g -PMMA IOL samples were significantly and permanently improved. Less platelets attachment was observed on the modified IOL, especially in the HEMA 2 - g -PMMA IOL group (with 1.65 × 10 −1 mol/L HEMA concentration), which also suppressed the proliferation of cells.
研究领域:
生物材料学
·
眼科学
·
表面科学
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生物医学工程
·
纳米医学
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DOI
高影响因子
COLLOID JOURNAL
·
2011
·Vol. 73
·期 1
·12-17
·
IF 0.622
Wang Yuhui,Ma Qianqian,Liu Li,Miao Jigen,Miao Yuqing
A novel technique of enzyme immobilization is developed for making the immobilized enzymes capable of further releasing for homogeneous reaction. Water-soluble poly(vinyl alcohol) was used to prepare enzyme-loaded membranes with immobilized acetylcholinesterase (AChE). When used, a piece of enzymecontaining membrane is put into the solution and dissolves quickly. The released AChE is mixed and interacts with substrates and carbaryl inhibitors. The catalytical activity and inhibition sensitivity of released enzyme are comparable to those of free AChE. The values of Michaelis constant and maximum reaction rate for released AChE are also very close to those for free AChE. The experimental conditions such as the concentrations of PVA and acetone, the time of enzymatic reaction and that of AChE inhibition by carbaryl pesticide were optimized. The relative inhibition of AChE activity increased with the carbaryl concentration ranging from 0.1 μg/L to 100 mg/L. When compared to free AChE in solution or solid powder, the prepared PVA-AChE membranes are advantageous with respect to storage and handling. The suggested technique of enzyme immobilization is suitable for the variety of applications, when the enzyme catalysed reactions allows for single-using of the active material and does not require further enzyme recovering.
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DOI
高影响因子
Biomed Research International
·
2015
·Vol. 2015
·期 1
·139179
·
IF 1.579
Jingjing Sun,Yiling Chen,Jun Sheng,Mi Sun
To improve the reusability and organic solvent tolerance of microbial lipase and expand the application of lipase (hydrolysis, esterification, and transesterification), we immobilized marine microbial lipase using different methods and determined the properties of immobilized lipases. Considering the activity and cost of immobilized lipase, the concentration of lipase was fixed at 2 mg/mL. The optimal temperature of immobilized lipases was 40°C and 5°C higher than free lipase. The activities of immobilized lipases were much higher than free lipase at alkaline pH (more than 50% at pH 12). The free lipase lost most activity (35.3%) and immobilized lipases retained more than 46.4% of their initial activity after 3 h heat treatment at 70°C. At alkaline pH, immobilized lipases were more stable than free lipase (more than 60% residue activity at pH 11 for 3 h). Immobilized lipases retained 80% of their activity after 5 cycles and increased enzyme activity (more than 108.7%) after 3 h treatment in tert-butanol. Immobilization of lipase which improved reusability of lipase and provided a chance to expand the application of marine microbial lipase in organic system expanded the application range of lipase to catalyze hydrolysis and esterification in harsh condition.
研究领域:
酶工程
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分子生物学
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生物催化
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工业微生物学
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蛋白质化学
·
应用酶学
✓ 已验证
DOI
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2023
·
IF 9.9
Minghui Xiong,Bo Chai,Guozhi Fan,Xiaohu Zhang,Chunlei Wang,Guangsen Song
The strong electron interaction between metal oxide–carbon-based catalyst components plays a vital role in the peroxymonosulfate (PMS) activation for pollutant degradation. Herein, a novel CoOOH nanosheets anchored on rape straw-derived biochar (BC) surface (labeled as CoOOH/BC) as an efficient PMS activator toward degrading sulfamethoxazole (SMX) was synthesized. Experimental results indicated that integrating CoOOH nanosheets on the BC surface could inhibit CoOOH aggregation to increase the specific surface areas, exert a component synergistic effect to enhance activation degradation activity, and improve the catalyst stability. As a result, a 96 % degradation efficiency of SMX was achieved within 20 min over 20 wt% CoOOH/BC composite catalyst under the optimal conditions. Density functional theory (DFT) calculations disclosed that a built-in electric field (BIEF) pointing from BC to CoOOH was constructed at their interface, which could mediate PMS activation for reactive oxygen species (ROS) generation and induce direct electron transfer from SMX to PMS, resulting in efficient SMX degradation via both radical and non-radical pathways. Moreover, quenching experiments and electron paramagnetic resonance (EPR) measurements confirmed that single oxide ( 1 O 2 ) and superoxide radical ( O 2 · - ) are the dominant active species in the current system. Additionally, the possible SMX degradation routes were reasonably proposed based on liquid chromatography-mass spectrometry (LC-MS) results. This work provides an in-depth understanding of the role of BIEF in PMS activation, and expands the application of biochar-based materials in the field of environmental remediation.
研究领域:
环境科学
·
材料化学
·
污染修复
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DOI
高影响因子
Bioresource Technology Reports
·
2021
·
IF 0
Ya Liu,Rui Pei,Zhongliang Huang,Jingran Xiao,Ayan Yao,Kaiji Xu,Yixin Li,Shafqat Ullah,Zongzhong Yu,Yuanpeng Wang,Shu-Feng Zhou,Guowu Zhan
Herein, we propose a facile synthetic strategy for biogenic photocatalyst by sequentially immobilizing CdS and Pt nanoparticles (NPs) on engineered recombinant Escherichia coli ( e-E. coli ) strains. In particular, we transferred the cysteine desulfurase gene to the heterologous host to overexpress cysteine desulfurase and the resulting e-E. coli strain could serve as a biofactory for the extracellular growth of CdS NPs involving catalyzing L-cysteine (Cys) to form a reactive sulfur source, followed by the precipitation of CdS via the reaction between Cd 2+ ions and the locally concentrated active sulfur source around the strain. The subsequent modification of e-E. coli /CdS composites with noble metal Pt NPs further enhanced the catalytic degradation performance towards organic dyes, due to suppressing the recombination of the photogenerated electron-hole pairs. Accordingly, the ternary e-E. coli /CdS/Pt composites emerged as robust photocatalysts for pollutant degradation in waste-water treatment, which is promising for environmental remediation in ambient conditions.
研究领域:
环境科学
·
生物技术
·
材料科学
✓ 已验证
DOI
JOURNAL OF HAZARDOUS MATERIALS
·
2022
·
IF 14.224
Xing Cao,Qiushi Zhang,Weichen Yang,Lin Fang,Shiwei Liu,Rui Ma,Kai Guo,Ning Ma
The high chlorine (Cl) and lead (Pb) content characteristics of municipal solid waste incineration fly ash (MSWIFA) pose environmental risks and hinder resource utilization. Herein, an MSWIFA-based magnesium potassium phosphate cement (MKPC) preparation strategy was developed, which allowed the MSWIFA recycling and the Pb-Cl synergistic immobilization without the washing pretreatment . The compressive strength of the resulting 10 wt% MSWIFA-based MKPC was 28.44 MPa, with over 99.2% reduction in leaching toxicity of Pb and Cl. The high-angle annular dark field scanning transmission electron microscope (HAADF-STEM) and X-ray absorption spectroscopy (XAS) analyzes showed that Pb, phosphate and Cl - formed Pb 5 (PO 4 ) 3 Cl in MKPC. In-situ X-ray diffraction (XRD) tests showed that Pb 3 (PO 4 ) 2 was gradually transformed to Pb 5 (PO 4 ) 3 Cl through a dissolution-precipitation process. The formation energy, Bader charge, charge density difference and density of states (DOS) of Pb 5 (PO 4 ) 3 Cl were analyzed by first-principles calculations, confirming that Pb 5 (PO 4 ) 3 Cl was more thermodynamically stable than Pb 3 (PO 4 ) 2 and PbCl 2 and that electronic interactions between Pb-p, O-p, P-p and Cl-p orbits were the origin of Pb-Cl synergistic immobilization. This work provides a new strategy for the resource utilization of MSWIFA without washing pretreatment, and provides an in-depth understanding of the Pb-Cl synergistic immobilization mechanism.
研究领域:
环境科学
·
材料科学
·
化学工程
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）