Cell Reports
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2022
·
IF 9.995
Xinyu Li,Jiasheng Li,Di Zhu,Ning Zhang,Xusheng Hao,Wenfeng Zhang,Qian Zhang,Yangli Liu,Xueying Wu,Ye Tian
Summary Coordination of inter-tissue stress signaling is essential for organismal fitness. Neuronal mitochondrial perturbations activate the mitochondrial unfolded-protein response (UPR mt ) in the intestine via the mitokine Wnt signaling in Caenorhabditis elegans . Here, we found that the protein disulfide isomerase PDI-6 coordinates inter-tissue UPR mt signaling via regulating the Wnt ligand EGL-20. PDI-6 is expressed in the endoplasmic reticulum (ER) and interacts with EGL-20 through disulfide bonds that are essential for EGL-20 stability and secretion. pdi-6 deficiency results in misfolded EGL-20, which leads to its degradation via ER-associated protein degradation (ERAD) machinery. Expression of PDI-6 declines drastically with aging, and animals with pdi-6 deficiency have decreased lifespan. Overexpression of PDI-6 is sufficient to maintain Wnt/EGL-20 protein levels during aging, activating the UPR mt , and significantly extending lifespan in a Wnt- and UPR mt -dependent manner. Our study reveals that protein disulfide isomerase facilitates Wnt secretion to coordinate the inter-tissue UPR mt signaling and organismal aging.
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TOXICOLOGY AND APPLIED PHARMACOLOGY
·
2020
·
IF 3.347
Yang Zhang,Jiabei Zhou,Qin Ye,Kui Zeng,Jie Pan,Lu Chen,Yingying Wang,Bo Yang,Qiaojun He,Jianqing Gao,Su Zeng,Lushan Yu
Thioredoxin 1 (Trx1) and telomerase play key roles in the development and progression process of most tumors, and they both are promising drug therapy targets. We have, for the first time, discovered that Trx1 and telomerase had a dual-target synergistic effect. Based on that results, we designed a series of 6-dithio-2′-deoxyguanosine analogs (named as YLS00X) and verified whether they can inhibit Trx1 and telomerase simultaneously. TrxR1/Trx1 system activity and telomerase expression were significantly inhibited by 6-dithio-2′-deoxyguanosine analogs, especially YLS004. YLS004 can also cause ROS accumulation, and induce tumor cell apoptosis . The vitro antitumor activity of 6-dithio-2′-deoxyguanosine analogs using MTT assay on 11 different human cancer cells and found that human colon cancer cells(HCT116) and melanoma cells (A375) were the most sensitive cells to 6-dithio-2′-deoxyguanosine analogs treatment and vivo xenografts models also confirmed that. The serum biochemical parameters and multiple organs HE staining results of subacute experiments indicated that YLS004 might be mildly toxic to immune organs, including the thymus, spleen, and hematopoietic system . Besides, YLS004 was rapidly metabolized in the rats' blood. Our study revealed that YLS004, a Trx1 and telomerase inhibitor , has strong anti-tumor effects to colon cancer and melanoma cells and is a promising new candidate drug.
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INTERNATIONAL JOURNAL OF LEGAL MEDICINE
·
2021
·Vol. 135
·期 2
·431-440
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IF 2.686
Jiang Bowei,He Wei,Jin Chuan,Liu Ying,Wen Dan,Wang Chudong,ZEYE Moutanou Modeste Judes,Li Jienan,Zha Lagabaiyila
Genotyping of short tandem repeat (STR) markers is the basic method of forensic science. Enhanced technologies are needed to meet the requirements of databasing and casework samples. The STRscan-17LC kit is a 6 Dye STR kit which amplifies 16 STR loci: D3S1358, TPOX, D16S539, vWA, D2S1338, CSF1PO, D19S433, D7S820, FGA, D8S1179, D5S818, D13S317, D18S51, TH01, D12S391, and D21S11 and the sex-determinant locus amelogenin. This kit is designed for better tolerance to PCR inhibitors and analysis of mildly degraded samples with all fragments smaller than 330 bases. In this study, the STRscan-17LC kit is validated according to the SWGDAM (Scientific Working Group on DNA Analysis Methods) guidelines, including PCR-based studies, sensitivity, precision and accuracy, inhibitors, species specificity, DNA mixture studies, population, and concordance studies. The validation results suggest that the STRscan-17LC kit is a useful tool for forensic application.
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Analytical Methods
·
2018
·Vol. 11
·期 2
·157-162
·
IF 2.073
Jimin Yang,Xuesong Yin,Wei Zhang
In our study, we report the development of molybdenum disulfide (MoS2) nanosheet-supported poly(indole-6-carboxylic acid) (PIn6COOH) and its usage for the electrochemical determination of circulating tumor DNA (ctDNA) such as PIK3CA gene associated with breast cancer. The MoS2 nanosheets prepared by the solvent exfoliation method from commercial bulk MoS2 were first immobilized on the surface of a carbon paste electrode; then, PIn6COOH, a novel electroactive material, was electropolymerized on the MoS2 substrate by the potentiostatic method to form a unique nanocomposite structure. The physical adsorption between aromatic In6COOH monomers and MoS2 efficiently improved the electropolymerization efficiency, resulting in increased electrochemical response of PIn6COOH. Owing to the presence of abundant carboxyl groups, probe ssDNA was covalently immobilized on the carboxyl-terminated PIn6COOH/MoS2 nanocomposite through free amines of DNA sequences based on 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide and N-hydrosulfosuccinimide crosslinking reaction. The covalently immobilized probe ssDNA could selectively hybridize with its target DNA to form dsDNA on the surface of the PIn6COOH/MoS2 nanocomposite. The self-signal changes of PIn6COOH induced by DNA immobilization and hybridization could be sensitively recognized via cyclic voltammetry and electrochemical impedance spectroscopy. This developed biosensor showed high performance of DNA hybridization with a detection limit of 1.5 × 10−17 mol L−1 for the detection of PIK3CA gene. The easy construction of this biosensor besides its fine sensitivity and very low time consumption can make this PIn6COOH/MoS2 nanocomposite very highly competitive for further applications in carcinoma diagnostic and therapeutic fields.
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MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 9
·1-13
·
IF 5.705
Wang Jiangru,Yu Jialuo,Wang Xiaoyan,Wang Liyan,Li Bowei,Shen Dazhong,Kang Qi,Chen Lingxin
Manganese-doped ZnS quantum dots (ZnS:Mn(II) QDs) were synthesized and modified with L-cysteine (L-Cys) and 6-mercaptonicotinic acid (MNA). This prevents the aggregation of the QDs and makes them available for the interaction with Cu(II) ions via Cu(II)-S interaction. As a result, the fluorescence of the QDs is quenched by Cu(II) due to an electron transfer mechanism. The QDs display two emission peaks under 325 nm excitation, one (being red) peaking at 593 nm, the other (blue) at 412 nm. The red fluorescence is strongly quenched, while the blue fluorescence is not affected. An easily distinguishable color change from orange red to purple can be observed in fluorescence as the concentration of Cu(II) is increased. The probe is selective over commonly encountered other ions. The ratio of fluorescence intensities at 593 and 412 nm increases linearly in the 5 to 500 nM Cu(II) concentration range, and the detection limit is 1.2 nM.
Schematic of the preparation of manganese-doped quantum dots functionalized with L-cysteine and 6-mercaptonicotinic acid for selective and sensitive visual detection of copper ions.
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Frontiers in Cellular and Infection Microbiology
·
2022
·
IF 6.073
Pi Xionge,Teng Weilin,Fei Dibo,Zhao Gang,Liu Wei
Probiotics, prebiotics, and synbiotics can alleviate metabolic syndrome by altering the composition of the gut microbiota. Live combined Enterococcus faecium and Bacillus subtilis has been indicated to promote growth and reduce inflammation in animal models. However, the modulatory effects of live combined B. subtilis R-179 and E. faecium R-026 (LCBE) on human microbiota remain unclear. The current study examined the growth of these two strains in the presence of various oligosaccharides and assessed the effects of this probiotic mixture on human and murine gut microbiota in vitro and in vivo. Oligosaccharides improved the growth of E. faecium R-026 and B. subtilis R-179 as well as increased their production of short-chain fatty acids. E. faecium R-026 or B. subtilis R-179 co-incubated with Bifidobacterium and Clostridium significantly increased the number of the anaerobic bacteria Bifidobacterium longum and Clostridium butyricum by in vitro fermentation. Moreover, LCBE significantly reduced plasma cholesterol levels in mouse models of hyperlipidemia. LCBE combined with galacto-oligosaccharides led to a significant decrease in the Firmicutes/Bacteroidetes ratio and a significant increase in the relative abundance of Akkermansia and Bifidobacteria after treating mice with LCBE (0.23 g/day) for eight weeks. Furthermore, in vitro fermentation also showed that both the single strains and the two-strain mixture modulated human gut microbiota, resulting in increased Lactobacillus and Bifidobacteria, and decreased Escherichia-Shigella. Overall, these results suggest that LCBE can improve host health by reducing the level of cholesterol in mouse models by modifying the composition of the gut microbiota.
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Nature Communications
·
2023
·Vol. 14
·期 1
·1-11
·
IF 16.6
Feng Yangyang,Li Yong,Lin Jing,Wu Huyue,Zhu Lei,Zhang Xiang,Zhang Linlin,Sun Chuan-Fu,Wu Maoxiang,Wang Yaobing
Stable lithium metal negative electrodes are desirable to produce high-energy batteries. However, when practical testing conditions are applied, lithium metal is unstable during battery cycling. Here, we propose poly(2-hydroxyethyl acrylate-co-sodium benzenesulfonate) (PHS) as negative electrode protective layer. The PHS contains soft poly (2-hydroxyethyl acrylate) and poly(sodium p-styrene sulfonate), which improve electrode flexibility, connection with the Cu current collector and transport of Li ions. Transmission electron cryomicroscopy measurements reveal that PHS induces the formation of a solid electrolyte interphase with a fluorinated rigid and crystalline internal structure. Furthermore, theoretical calculations suggest that the -SO 3 - group of poly(sodium p-styrene sulfonate) promotes Li-ion motion towards interchain migration through cation-dipole interaction, thus, enabling uniform Li-ion diffusion. Electrochemical measurements of Li | |PHS-coated-Cu coin cells demonstrate an average Coulombic efficiency of 99.46% at 1 mA/cm 2 , 6 mAh/cm 2 and 25 °C. Moreover, when the PHS-coated Li metal negative electrode is paired with a high-areal-capacity LiNi 0.83 Co 0.11 Mn 0.06 O 2 -based positive electrode in multi-layer pouch cell configuration, the battery delivers an initial capacity of 6.86 Ah (corresponding to a specific energy of 489.7 Wh/kg) and, a 91.1% discharge capacity retention after 150 cycles at 2.5 mA/cm 2 , 25 °C and 172 kPa.
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ChemistrySelect
·
2023
·Vol. 8
·期 33
·e202301702
·
IF 2.1
Zhiwei Chen,Xiaoxiao Huang,Luyao Yang
Graphical Lewis Acid-Mediated One-pot Tandem Reactions for synthesis of structurally diverse Furo[3,2-c]coumarins. The three-component cyanoalkylsulfonylation cyclization reaction of N -methacryloyl-2-phenylbenzimidazole, DABCO ⋅ (SO 2 ) 2 and cyclobutanone oxime ester regulated by different metals (iron and nickel) and the two-component cyanoalkylation cyclization reaction of DABCO ⋅ (SO 2 ) 2 as oxidant were described. The tandem cyclization route allows different cyanoalkylsulfonylated benzimidazole [2,1-a] iso-quinoline-6(5H)-ones/cyanoalkylated benzimidazole [2,1-a] iso-quinoline-6(5H)-ones to be obtained in moderate to good yields under mild conditions, with good functional group tolerance, readily available starting materials, and simple operation. In addition, experiments show that the tandem cyclization reaction was a free radical reaction process.
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MICROCHIMICA ACTA
·
2021
·Vol. 188
·期 5
·1-9
·
IF 5.833
An Xuanxuan,Chen Ruchun,Chen Qizhen,Tan Qin,Pan Shuang,Liu Hui,Hu Xiaoli
A MnO 2 nanosheet-assisted ratiometric fluorescence probe based on carbon quantum dots (CQDs) and o-phenylenediamine (OPD) has been developed for the detection of the anticancer drug 6-mercaptopurine (6-MP). CQDs with strong fluorescence are synthesized via the one-step hydrothermal method. MnO 2 nanosheets as an oxidase-mimicking nanomaterial directly oxidize OPD into 2,3-diaminophenazine (DAP) which has a fluorescence emission at 570 nm, whereas the fluorescence of CQDs at 445 nm is then reduced by the DAP through the inner filter effect (IFE) under a single excitation wavelength (370 nm). After adding 6-MP, MnO 2 nanosheets can be reduced to Mn 2+ and lose their oxidase-like property, blocking the IFE with the fluorescence decrease of DAP and fluorescence increase of CQDs. The novel ratiometric fluorescence probe exhibits considerable sensitivity toward 6-MP and linear response is in the 0.46–100.0 μmol L −1 concentration range with the detection limit of 0.14 μmol L −1 . Furthermore, the probe shows good selectivity when exposed to a series of interfering other organic and inorganic compounds, and biomolecules and can be applied to the detection for 6-MP in human serum samples and pharmaceutical tablets. Satisfactory recoveries of 6-MP in human serum samples are in the range 96.1–110.9% with the RSD of 1.4 to 3.2%. The amount of 6-MP is successfully estimated as 49.3 mg in pharmaceutical tablet with the RSD of about 2.2%. Graphical abstract
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Annals of Medicine and Surgery
·
2023
·Vol. 85
·期 11
·5469
·
IF 1.7
Ren Yuzhe,Li Xing,Zhang Yuanjie,Yan Zilong
Xiaoqinglong decoction (XQLD) is widely used clinically in the treatment of childhood cough variant asthma (CVA). However, its potential mechanism is still unknown. In the present study, the authors investigate the biological network and signalling pathway of XQLD in treatment of childhood CVA using network pharmacology-based analysis and experimental validation. By using the Bioinformatics Analysis Tool Molecular Mechanism of Traditional Chinese Medicine (BATMAN-TCM) database, the authors confirmed the correlation between XQLD and asthma, and the authors screened 1338 potential target genes of Mahuang and Guizhi, the most active herbs in XQLD. By overlapping “Childhood asthma-related genes” of DisGeNET database, the authors identified 58 intersecting genes of Childhood asthma and 1338 target genes of Mahuang and Guizhi. The intersecting genes were used to construct the protein-to-protein interaction and performed Gene Ontology (GO) functional and the Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses. Gene Ontology enrichment analysis demonstrated 359 Biological Process terms, 16 Cellular Component terms, and 26 Molecular Function terms. Meantime, 75 terms of Kyoto Encyclopedia of Genes and Genomes signalling pathway were involved in enrichment analysis. These candidates showed a significant correlation with inflammatory response and positive regulation of tyrosine phosphorylation of STAT protein. In addition, XQLD treatment significantly upregulated serum interferon-γ expression, and downregulated serum interlukin-6 expression of CVA mice. XQLD treatment significantly inhibited phosphorylation of STAT3 in bronchial-lung tissues. Our data suggest that XQLD effectively alleviated bronchial-lung tissue damage in CVA mice and inhibited the body inflammatory response by regulating interlukin-6/STAT3 signalling pathway.
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POLYMERS FOR ADVANCED TECHNOLOGIES
·
2021
·Vol. 32
·期 6
·2508-2516
·
IF 3.665
Tao Zheng,Wengui Wang,Yaochi Liu
In this work, a novel phosphorus–nitrogen flame retardant (CN-DOPO), which was successfully prepared via a facile method of graphitic carbon nitride (g-C 3 N 4 ) and 9,10-dihydro-9-oxa-10-phosphaphenanthrene-10-oxide (DOPO). Polyamide 6 (PA6) composites were then prepared through melt blending with different ratios of g-C 3 N 4 /DOPO and different contents of CN-DOPO. CN-DOPO was characterized and confirmed using X-ray diffraction (XRD), Fourier transform infrared spectra (FTIR), and scanning electron microscopy (SEM). PA6/CN-DOPO composites were investigated by limiting oxygen index (LOI), vertical combustion (UL-94), thermal gravimetric analysis (TGA), differential scanning calorimetry (DSC), and thermogravimetric analysis-infrared spectrometry (TGA-IR). The results indicated LOI value was increased from 23.4% to 29.2% and successfully passed UL-94 V-0 rating when the presence of CN-DOPO increased to 15 wt%. Moreover, mechanical properties of PA6/CN-DOPO composites had not deteriorated significantly due to CN-DOPO having better dispersion and compatibility in PA6. The morphological of char layer revealed that CN-DOPO formed a highly integrated char layer surface during the combustion process, as compared to that of pure PA6. Meanwhile, CN-DOPO decomposed to release gases could inhibit decomposition by diluting flammable volatiles. Accordingly, the special gas-condensed phase synergistic flame-retardant mechanism of PA6/CN-DOPO was proposed. This work indicated that CN-DOPO could endow PA6 with highly effective flame-retardant and good mechanical performance.
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CHEMICAL ENGINEERING JOURNAL
·
2021
·
IF 13.273
Xin Liu,Qi Fu,Peng Xu,Pengfei Zhu,Zhuoyu Yang,John C. Crittenden
A sensitive spectrophotometric method (the Br − -ABTS method) for measuring monopersulfate (PMS) of 0–20 μM in water was established. The Br − -ABTS method was based on the oxidation coloration reaction where Br − as catalyst promoted PMS for oxidation of ABTS forming the green-colored ABTS radical (ABTS • + ) under pH 3.0 (50 mM phosphate buffer). ABTS • + could be quantitatively measured at 415 nm, 650 nm, 732 nm and 820 nm using UV–vis spectrophotometer. There was a well linear relationship between the absorbance of ABTS • + and PMS concentration with the value of R 2 being 0.999. The proposed Br − -ABTS method was highly sensitive, and the absorbance generated by Br − -ABTS at 415 nm was 6.59 × 10 4 cm −1 per M (mol L –1 ) of PMS. The changes of pH from 3.0 to 9.0 had an insignificant effect on the calibration curve of Br − -ABTS method. The reaction of PMS with ABTS had a stoichiometric coefficient of 1:1.96 at the pH 3.0. The residual absorbance of ABTS •+ in ultrapure water, Xiangjiang water and Hou-lake water was stable within 30 min. Moreover, the trace PMS concentration of 0.03–20 μM in ultrapure water, 0.05–20 μM in natural water (e. g. Xiangjiang water and Hou-lake water) and 1–20 μM in wastewater was accurately measured with the proposed Br − -ABTS method. Additionally, the interference of natural water matrix (e. g. humic acid), metal ions (e. g. Co(II), Fe(III) and Cu(II)) and hydroxylamine had barely interference for the proposed Br − -ABTS method. Furthermore, the Br − -ABTS method was more accurate and flexible to determine PMS concentration than DPD method during the decolorization of rhodamine B in the Co(II)/PMS system, Cu(II)/PMS system and hydroxylamine/Fe(III)/PMS process.
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