MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Mingbing Yi,Wenjun Liu,Cong Wang
Lactate, a critical biomarker for various physiological and pathological conditions, necessitates precise quantification methods for effective disease diagnostics and monitoring. Traditional detection techniques, though effective, are often hampered by the need for complex instrumentation and procedures. Here, this study introduces a highly sensitive and selective electrochemiluminescence (ECL) aptasensor for the detection of lactate, leveraging the unique properties of DNA-terbium (Tb) nanoparticles (NPs) and aptamer technology. Specifically, in this label-free ECL sensor, DNA-Tb NPs were utilized as both ECL probes and recognition unit, which were synthesized by a simple coordination driven self-assembly of lactate aptamer ligand with terbium ions metal center. The biosensor exhibits a remarkable limit of detection (LOD) of 2.9 μ M across a linear range of 0.01 to 10 mM, demonstrating high selectivity, stability, reproducibility, and practical application potential. The work not only underscores the efficacy of DNA-Tb NPs in enhancing ECL signals but also showcases the aptamer’s role in achieving high specificity towards lactate detection. The optimization of assay conditions further elevates the biosensor’s performance, establishing it as a promising tool for the rapid, simple, and sensitive detection of lactate in various settings.
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FOOD CHEMISTRY
·
2024
·
IF 8.5
Yongfu Pu,Zhijia Gu,Toine F.H. Bovee,Ying Yang,Yu Ying,Maokang Li,Xiaodi Hong
A novel superparamagnetic photonic crystal DNA probe (Fe 3 O 4 @SiO 2 @amino@DNA SPC) was developed to enable rapid visual detection of Hg 2+ . This unique photonic crystal (PC) was synthesized by combining superparamagnetic nanospheres with DNA probes. The DNA probe, rich in thymine (T), detects mercury ions through base mismatch, resulting in the formation of T–Hg 2+ –T loop hairpin structures. With the binding of Hg 2+ to the probe attached to superparamagnetic nanospheres, the PC structure assembled by these nanospheres, formed by the magnetic field, was changed. This change enhanced the reflection intensity; it could be quantified using a fiber optic spectrometer and was visible to the naked eye. The Fe 3 O 4 @SiO 2 @amino@DNA SPC, specific to Hg 2+ , exhibited a reflection peak at 679 nm, which intensified with increasing Hg 2+ concentration. The reflection intensity increased by 132.58 a.u., and the PC color shifted from red to yellow as the Hg 2+ concentration increased from 0.1 μg/L to 1 mg/L.
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CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Xiangyang Li,Min Yang,Yushi Xie,Longjiao Zhu,Wentao Xu
Every year, a significant amount of fruit and vegetable spoilage caused by microbial contamination leads to substantial food waste and economic losses in the agricultural and food sectors. There is an urgent need to develop highly efficient and safe multifunctional preservation materials. Curcumin (Cur) is known for its excellent antioxidant and antibacterial properties; however, its poor water solubility and low bioavailability severely limit its practical applications. In this study, we synthesized highly stable and water-dispersible metal-biomolecule network nanoparticles by self-assembly of ferrous ions (Fe 2+ ) with DNA in an aqueous solution at room temperature. Furthermore, by leveraging the strong coordination interaction between Iron(Fe) and Cur, we prepared Cur-modified nanoparticles (Fe-DNA-Cur) with dual antioxidant and antibacterial functions. Fe-DNA-Cur not only preserves the biological activity of curcumin, but also effectively addresses its inherent poor water solubility. After further compounding with chitosan (CS), the as-prepared Fe-DNA-Cur@CS composite demonstrates breakthrough antimicrobial performance, thereby providing an innovative solution for developing novel, highly effective and safe fruit/vegetable preservatives. When used as a sustainable food packaging coating for perishable fruits at room temperature, the Fe-DNA-Cur@CS coating can reduce dehydration, prevent nutrient loss, inhibit microbial growth, increase nutraceutical value, maintain strawberry quality, and extend shelf life during storage. This study demonstrates the potential of Fe-DNA-Cur@CS as a multifunctional coating material to reduce food waste and agricultural economic losses caused by microbial contamination.
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TALANTA
·
2025
·
IF 6.1
Jing Luo,Nan Jia,Jiangshan Xue,Nan Zhang,Le Li,Yongli Wu,Mengyan Lei,Jiahui Zhang,Xiangyuan Ouyang,Huaiyu Bu
Staphylococcus aureus is a common foodborne pathogen that poses a serious threat to public health, necessitating the development of simple, ultrasensitive, and highly selective detection methods. In this study, we designed a colorimetric sensor by incorporating SA 31 aptamers with DNA nanosheet-templated copper nanoclusters (DNS/CuNCs) for the precise detection of S. aureus . The DNS/CuNCs exhibit peroxidase-like activity, catalyzing the oxidation of TMB to ox-TMB, resulting in a color change directly correlated with the bacterial concentration. This bioassay demonstrated a broad detection range of 10-10 7 CFU/mL and an impressive detection limit of 3 CFU/mL, surpassing most previous reports. In addition, DNS/CuNCs-SA 31 biosensor effectively detected S. aureus at low concentration as low as 58 CFU/mL in practical samples including drinking water, orange juice, and milk using a smartphone-based readout. The study introduces a novel approach for the sensitive and selective detection of S. aureus leveraging the enzyme-like activity of DNA nanostructures, offering a convenient and efficient solution of real-world pathogen monitoring.
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JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2025
·
IF 4.6
Wen Yun,Youwei Chen,Ruiqi Wang,Nana Xie,Xingmin Wang
A novel spatially constrained dual pincer DNA scaffold was developed for Bisphenol A (BPA) detection. The dumbbell-shaped aptamer hairpin could be opened by target BPA. The two tails of opened aptamer-hairpin separately triggered the catalytic hairpin assembly between the two hairpins at both ends of DNA scaffold through the proximity effect. The effective concentration of the hairpins on dual pincer DNA scaffold greatly increased about 2800 fold due to the spatial constraint design. A good dynamic correlation was shown from 10 pM to 300 pM. The limit of detection was as low as 3 pM according to the 3σ/slope protocol. Real sample detection confirmed that dual pincer DNA scaffold could be effectively used for food and environmental monitoring with accurate sensing.
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Nature Communications
·
2025
·Vol. 16
·期 1
·1-12
·
IF 15.7
Chen Yonghuan,Li Xiuying,Yue Xinru,Yu Weihua,Shi Yuesen,He Zilong,Wang Yuanfeng,Huang Yu,Xia Fan,Li Fengyu
Synthetic drugs fundamentally reshape the illicit drug market due to their low cost, ease of production, and rapid manufacturing processes. However, current drug detection methods, which rely on complex instruments, have limited applicability and often neglect the influence of pH fluctuations, leading to potential bias and unreliable results. Herein, we propose co-calibration DNA probes on a nanoconfined biosensor (N C B S ), covering the range of sweat pH 3–8 to achieve significantly enhanced target signal recognition. The N C B S exhibits a linear response range of 10 3 -10 8 fM with a low limit of detection (LOD) of 3.58 fM in artificial sweat. Compared to the single-aptamer N C B S , the dual-aptamer N C B S offers a broader linear response range, primarily due to the synergistic effects of changes in surface wettability and the capture of hydrion, which together reduce signal interference in proton transport. The linear response range doubles, and its detection sensitivity improves by 4–5 orders of magnitude compared to existing drug detection methods. This sensing strategy expands the application scope of aptamer-based composite probes, offering an approach for ultra-sensitive drug detection and demonstrating significant potential in sweat sensing and drug monitoring fields.
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Matter
·
2024
·
IF 17.3
Yi Zhang,Weiwei Shi,Chao Ma,Sikang Wan,Cong Li,Jianlei Shen,Jingjing Li,Xiaoguo Liu,Dong Chen,Fan Wang,Hongjie Zhang,Chunhai Fan,Kai Liu
The impressive mechanical properties of DNA molecules play important physiological and pathological roles, which arise from their
intrinsically ordered and periodic double-helix structures. Nevertheless, bulk fabrication of DNA-based materials that inherit their nanoscale mechanical strength remains a grand challenge. Here, we
developed a multiscale engineering approach to assemble with
highly ordered DNA bulk fibers. We found that the involvement of
metal ions enabled tunable assembly and orientation of DNA-metal
ensembles ranging from molecules to nanobundles and to macroscale filaments. This strategy remarkably enhanced the mechanical
performance of DNA fibers with the optimal tensile strength and
toughness reaching to 513 MPa and 130 MJ m3
, respectively,
which outperform even partially protein- and polymer-based fibers.
We further demonstrated the high scalability of this method by
continuous spinning of 1,000 m of robust fibers with 50 mg DNA,
which shows great promise in translations of DNA-based materials
in mechanobiology, bioelectronics, and beyond.
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RSC Advances
·
2024
·Vol. 14
·期 14
·9602-9608
·
IF 3.9
Tiantian Wu,Yanqiang Shi,Tao Yang,Pengxuan Zhao,Zhu Yang,Bin Yang
Dolastatin derivatives possess excellent anticancer activity and have been translated into clinical trials for cancer therapy. Drug delivery systems enable dolastatin derivatives to break the limitation of instability during blood circulation and ineffective cell internalization in the application. Nevertheless, their potential has not been thoroughly established because of the limited loading efficacy and complicated chemical modification. Herein, we rationally propose a rolling circle amplification-based polymer–DNA assembled nanoflower for targeted and efficient delivery of dolastatin-derived drugs to achieve efficient anticancer therapy. The polymer–DNA assembled nanoflower with targeted aptamer conjugate is widely applicable for loading dolastatin-derived drugs with high encapsulation efficiency. The developed monomethyl auristatin E (MMAE) loaded PN@M exhibited increased cellular uptake and enhanced inhibitory effect, especially in multidrug-resistant tumor cells. The results of in vivo anticancer effects indicate that nanoflower as a dolastatin derivatives delivery system holds considerable potential for the treatment of malignant cancer.
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IEEE SENSORS JOURNAL
·
2024
·Vol. 24
·期 24
·40904-40911
·
IF 4.3
Xuegang Li,Pengqi Gong,Xue Zhou
Deoxyribonucleic acid (DNA) detection represents an important direction of diagnosis and treatment of genetic diseases, which contains all the genetic information of the human body. The DNA sequence remains extremely challenging to detect, despite numerous previous studies that have achieved selective detection, due to the backward sensing scheme. For low-cost, high-sensitivity DNA hybridization detection, a probe-type highly sensitive DNA detection method based on surface plasmon resonance (SPR) is demonstrated in this article. First, low-cost refractive index (RI) tracking detection can be completed via optical fiber SPR. Then, the selective detection of complementary DNA (cDNA) is achieved by binding the probe DNA (pDNA) sequence to the optical fiber SPR sensor using polyethyleneimine (PEI). Compared with previous studies, the sensitivity [0.13 nm/(nmol/L)] is greatly upgraded, and the detection limit (DL) is reach to 4.87 nmol/L. This low-cost and high-performance optical fiber sensing method is conducive to the further development of DNA hybridization detection technology.
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CHINESE JOURNAL OF CHEMISTRY
·
2025
·
IF 5.5
Mengyao Cao,Xiewei Xiong,Li Li,Hao Pei
Comprehensive Summary Monitoring and analyzing expression levels of multiple biomarkers in biological samples can improve disease risk prediction and guide precision medicine but suffers from high cost and being time-consuming. Here, we construct a fast molecular classifier based on freeze-thaw cycling that implements an in silico support vector machine (SVM) classifier model at the molecular level with a panel of disease-related biomarkers expression patterns for rapid disease diagnosis. The molecular classifier employs DNA reaction networks as the computing module and repeated dehydration and concentration process as the driving force to implement a set of simplified mathematical operations (such as multiplication, summation and subtraction) for efficient classification of complex input patterns. We demonstrate that the fast DNA-based molecular classifier enables precise cancer diagnosis within a short turnaround time in synthetic samples compared to those of free diffusion classifiers. We envision that this all-in-one molecular classifier will create more opportunities for inexpensive, accurate, and rapid disease diagnosis, prognosis and therapy, particularly in emergency departments or the point of care.
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CHEMICAL ENGINEERING JOURNAL
·
2021
·
IF 13.273
Zhenqiang Fan,Bo Yao,Yuedi Ding,Dong Xu,Jianfeng Zhao,Kai Zhang
Fast and effective detection of epidemics is the key to preventing the spread of diseases. In this work, we constructed a dual-wavelength ratiometric electrochemiluminescence (ECL) biosensor based on entropy-driven and bipedal DNA walker cycle amplification strategies for detection of the RNA-dependent RNA polymerase (RdRp) gene of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The entropy-driven cyclic amplification reaction was started by the SARS-CoV-2 RdRp gene to generate a bandage. The bandage could combine with two other single-stranded S1 and S2 to form a bipedal DNA walker to create the following cycle reaction. After the bipedal DNA walker completed the walking process, the hairpin structures at the top of the DNA tetrahedrons (TDNAs) were removed. Subsequently, the [email protected] 3 C 2 @AuNPs-S7 probes were used to combine with the excised hairpin part of TDNAs on the surface of Au-g-C 3 N 4 , and the signal change was realized employing electrochemiluminescence resonance energy transfer (ECL-RET). By combining entropy-driven and DNA walker cycle amplification strategy, the ratiometric ECL biosensor exhibited a limit of detection (LOD) as low as 7.8 aM for the SARS-CoV-2 RdRp gene. As a result, detecting the SARS-CoV-2 RdRp gene in human serum still possessed high recovery so that the dual-wavelength ratiometer biosensor could be used in early clinical diagnosis.
研究领域:
生物技术
·
分析化学
·
分子生物学
·
纳米技术
·
病毒学
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SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Kai Wen,Xingdong Yang,Cuiling Zhang,Huiying Wei,Guizheng Zou,Ye Zhu
Diffusion-mediated homogeneous electrochemical assay has attracted substantial attention owing to its simplicity, rapid response, and high recognition efficiency, however, lack of inherent connection between signal reporter and electrode surface restricts the improvement of detection sensitivity and further limits its potential applications. Herein, a facile electrochemical strategy is proposed through integrating the advantages of traditional heterogeneous assay and homogenous assay. In this strategy, a small amount of target DNA can trigger the release of assist DNA from the electrode surface. Then, the assist DNA can induce the homogeneous amplification reaction, which in turn promotes the amplification on the electrode surface by generating numerous target DNA analogues, thereby realizing positive feedback signal amplification. As a result, ultrasensitive electrochemical detection of nucleic acids was achieved in the dynamic range from 5 aM to 5 fM with a detection limit down to 1.2 aM. There is about 2.5 orders of magnitude improvement in sensitivity compared with the control strategy without homogeneous amplification. Moreover, the proposed strategy was demonstrated with high selectivity, reproducibility, and capability of detecting nucleic acids in serum samples, displaying its promising potential in the development of ultrasensitive electrochemical biosensing platform for disease diagnosis applications.
研究领域:
生物化学
·
分析化学
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