Innovative Food Science & Emerging Technologies
·
2023
·
IF 6.6
Wenhui Zou,Huihui Niu,Junjie Yi,Linyan Zhou
This study evaluated the changes of enzymes and quality properties of passion fruit juice juicing with (PFJ-1) and without seeds (PFJ-2) treated by high-pressure processing (HPP, 600 MPa/5 min) and thermal pasteurization (TP, 85 °C /30 s) during storage at 4 °C for 35 days. Juicing with seeds resulted in PFJ-1 with 76.83, 68.25 and 28.12% higher activities of polyphenol oxidase , peroxidase and superoxide dismutase than PFJ-2, respectively, and its apparent viscosity at 10 −1 s and L * were also 148.60 and 1.47% higher, respectively. The total sugars, organic acids, and volatile compounds in PFJ-1 were 9.16, 4.97 and 53.85% higher than PFJ-2, respectively. Quality properties, including sugars, organic acids, volatile compounds in PFJ-1-HPP were all more susceptible towards degradation than PFJ-2-HPP under storage, which were decreased by 21.93, 26.14 and 18.47% after storage. This research provided new insights for production of high-quality passion fruit juice. Industrial relevance At present, the passion fruit juice is the dominated product in passion fruit industry, but there are few studies reported on the difference between the juice quality under different juicing methods by juicing with or without seeds. The seeds in passion fruit contains a great variety of substances such as oxidative browning enzymes, unsaturated fatty acids , which can significantly affect the quality of passion fruit juice after entering in juice. This research is of great significance as a theoretical basis to further research on the influence mechanism of enzyme and phytochemicals in seeds on the quality of passion fruit juice, and also provides a guide to production for quality orientated passion fruit juice based on consumer and producer demands.
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Analytical Methods
·
2018
·Vol. 10
·期 10
·1247-1257
·
IF 2.073
Jun Sang,Bing Li,Ya-ya Huang,Qun Ma,Kang Liu,Cui-qin Li
This study aimed to extract and separate total anthocyanins from Lycium ruthenicum Murr. by combining deep eutectic solvents (DES) with macroporous resin chromatography and to develop green analytical methods for the determination of anthocyanins. Choline chloride/1,2-propanediol (1 : 2, mol mol−1) containing 10% water (v/v) was screened as the optimal extraction solvent, and extraction at 52 °C for 45 min using a liquid/solid ratio of 20 : 1 gave the maximum extraction yield of total anthocyanins (4.45 ± 0.07 mg g−1). According to the static/dynamic adsorption and desorption tests, LS-32 macroporous resin has the ability to separate anthocyanins from DES with a recovery ratio above 95%. An off-line heart-cutting two-dimensional HPLC-DAD/ESI-MS method was developed for the qualitative and quantitative analysis of L. ruthenicum anthocyanins using environmentally friendly mobile phases. Ethanol and a tartaric acid solution (0.3 mol L−1) were used as the mobile phases of the first-dimensional HPLC-DAD method, and 15 anthocyanin peaks were detected within 17 min. The second-dimensional HPLC-MS method was used to identify the main anthocyanin peaks in the first dimension. Method validation showed that the developed method was accurate, stable and reliable for the analysis of L. ruthenicum anthocyanins. The present study provides an excellent alternative green method for the preparation and determination of anthocyanins from plant material.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Jun Li,Pingcong Lv,Hongli Ou,Xianling Yang,Youyi Chang,Yanli Li,Qian Zhou,Yimin Shan,Xianggui Chen,Xiaoqi Yu
The slight acidic pH changes during food storage and transportation are often difficult to detect in a timely and accurate manner. There is also an urgent need to establish a portable, accurate, and low interference detection method for real-time monitoring of the quality changes of fresh juice that already has its own color. In this paper, a self-ratiometric fluorescent sensor (DCCA/SA bead) was prepared by combining a modified coumarin and sodium alginate (SA), and was used for the extreme pH monitoring of fluorescent by naked eye colorimetry. The derivative of natural active ingredient coumarin (DCCA) exhibited excellent self-fluorescence properties in the ratio of orange red (630 nm) to blue-green (480 nm) under the excitation, and exhibited good linear changes at extreme acid pH range (2.5–5.0), biocompatibility high sensitivity, and anti-interference ability. Based on the multiple interactions between DCCA and SA, DCCA/SA bead could be used for the monitoring small pH changes of fruit juice and storage stage in a fast response time. In addition, analyzing the RGB values of DCCA/SA bead images under ultraviolet light also successfully quantitatively determined changes in pH values. These results indicated that the self-ratiometric fluorescent hydrogel sensor had great user convenience and point-of-care testing (POCT) to monitor extreme acidic pH for freshness of foods.
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高影响因子
Analytical Methods
·
2024
·Vol. 16
·期 29
·4951-4959
·
IF 2.7
Weiqin Zhao,Ruichen Zhai,Qianxiao Chen,Chun Huang,Haojia Li,Youyu Zhu,Yingfeng Duan,Jie Gao
Glutathione (GSH) is commonly used as a diagnostic biomarker for many diseases. In this study, based on carbon quantum dots prepared from dragon fruit peel (D-CQDs) and the T-Hg(II)-T mismatch, a dual-mode biosensor was developed for the detection of GSH. This system consists of two single-stranded DNA (ssDNA). DNA1 was the T-rich sequence; DNA2 was attached to streptavidin-coated magnetic beads and consisted of T-rich and G-rich fragments. Due to the presence of Hg(II), the T-Hg(II)-T mismatch was formed between T-rich fragments of two ssDNA. In the presence of GSH, Hg(II) detached from dsDNA and bound with GSH to form a new complex. The G-rich fragment assembled with the hemin shed from D-CQDs to form the G-quadruplex/hemin complex. At this time, in fluorescence mode, the fluorescence of D-CQDs quenched by hemin could be restored. In colorimetric mode, after the magnetic beads separate, a visual signal could be produced by catalyzing the oxidation of ABTS using the peroxide-like activity of the G-quadruplex/hemin complex. This biosensor in both fluorescence mode and colorimetric mode had excellent selectivity and sensitivity, and the limit of detection was 0.089 µM and 0.26 µM for GSH, respectively. Moreover, the proposed dual-mode biosensor had good application prospects for detection of GSH.
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高影响因子
LUMINESCENCE
·
2025
·Vol. 40
·期 2
·e70106
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IF 3
Mingyu Zhang,Yizhuo Tian,Sijia Liu,Yu Wang,Haiyan Li,Yafei Chen,Qing Gao,Xinli Wang,Mingli Chen
A multifunctional surface-enhanced Raman scattering substrate Fe 3 O 4 @AgNPs@MIL-101 was prepared. Rapid SERS detection of pesticide residues was realized by direct pre-enrichment and separation on the peel surface. MIL-101 has an ortho-octahedral framework and large pore size, which endowed Fe 3 O 4 @AgNPs@MIL-101 with the ability to rapidly adsorb and separate positively charged targets. The introduction of tannic acid realized the in situ growth of AgNPs on the backbone, to modulate the electromagnetic enhancement. Pesticide molecules were adsorbed onto the surface of AgNPs mediated by central S atoms, accompanied by the interaction between pesticide molecules and AgNPs, the corresponding SERS signals of different pesticides were observed. Together with the introduction of magnetic coating Fe 3 O 4 , the molecules were enriched in the hotspot and separated to further enhance the SERS performance. Magnet instead of centrifugation was used to simultaneously perform surface extraction and sample separation for a noninvasive, rapid, immediate, and portable assay. The method was accomplished in measurement of thiram and thiabendazole on apple and tangerine epidermis, and the limits of detection (LODs) were 20 ng/cm 2 and 4 μg/cm 2 , respectively. The recovery was reasonable, and it showed that the procedure is valuable for the rapid and nondestructive surface analysis of residual chemicals.
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POSTHARVEST BIOLOGY AND TECHNOLOGY
·
2013
·
IF 2.454
Jianwen Bu,Yucong Yu,Guzhanuer Aisikaer,Tiejin Ying
Mature green cherry tomato fruit were harvested and treated with ultraviolet-C (UV-C) irradiation at a predetermined dose of 4.2 kJ m −2 and stored at 18 °C for 35 days. The effects of UV-C treatment on firmness, cell wall compositions (pectin and cellulose), cell wall ultra-structure, levels of ethylene production, and activities of cell wall degrading enzymes in the fruit were investigated during storage. Major genes involved in cell wall degradation ( PME 2.1, Cel 1, PG cat and Exp 1) were also checked. Fruit firmness was better maintained in UV-C treated fruit corresponding with higher contents of cellulose and acid-soluble pectin. Transmission electron microscopy indicated that UV-C irradiation retarded cell wall disassembly in the pericarp. Ethylene production was significantly inhibited by UV-C treatment. The UV-C treatment also suppressed the transcriptional expression of PME2.1 , Cel1 , PGcat and Exp1 genes, and inhibited pectin methylesterase (PME; EC 3.1.1.11), polygalacturonase (PG; EC3.2.1.15) and cellulase (EC3.2.1.4) activities during storage. These results suggested that the inhibition of ethylene production, which in turn down-regulated expression of genes encoding cell wall degrading enzymes, might be one of the possible mechanisms of UV-C delaying tomato fruit softening.
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Food Hydrocolloids for Health
·
2025
·
IF 5.1
Yingzhu He,Ze Ma,Hejing Shen,Bei Fan,Xiang Li,Qin Tian,Fengzhong Wang,Yajuan Bai
The bioactivity of Alpinia oxyphylla fruit polysaccharides (AFP) is closely linked to their extraction-determined structural properties. This study systematically compared three extraction methods (ultrasound-assisted enzymatic (AFP-UE), pure ultrasound (AFP-U), and hot water (AFP-H)) for their efficiency in extracting AFP and influencing their structural features and intestinal barrier protective effects. Although AFP-UE yielded the highest extraction efficiency (4.75%) and exhibited predominantly a neutral arabinogalactan, it was AFP-U (comprises mixed rhamnogalacturonan-I and homogalacturonan domains alongside notable glucan motifs) that demonstrated the most potent intestinal barrier protection in a dextran sulfate sodium (DSS)-induced zebrafish colitis model. At 50 μg/mL, AFP-U significantly reduced the pro-inflammatory cytokine TNF-α, while upregulating anti-inflammatory cytokine IL-4, tight junction protein ZO-1, and mucin 2. Mechanistically, AFP-U is very likely to mainly alleviated oxidative stress and promoted intestinal barrier repair primarily by activating the IL-22 pathway, as evidenced by enhanced IL-22/IL-10 expression and suppression of IL-22 Receptor Subunit Alpha-2 (IL-22RA2). These findings highlight the critical role of extraction methods in shaping polysaccharide structure and bioactivity, positioning pure ultrasound extraction as a green and efficient strategy for producing functional polysaccharides with enhanced gastrointestinal health benefits.
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JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
·
2019
·
IF 4.978
Atian Xie,Jiuyun Cui,Yangyang Chen,Jihui Lang,Chunxiang Li,Yongsheng Yan,Jiangdong Dai
Realization of fast, efficient and continuous oil–water separation is of great significance for solving the problem of oil pollution. We present here a facile hydrothermal strategy to synthesize a superhydrophilic platanus fruit-like Ni@Ni(OH) 2 hierarchical architecture (SI-PFHA). Additionally, superhydrophobic platanus fruit-like Ni@Ni(OH) 2 hierarchical architecture (SO-PFHA) is obtained through stearic acid modification of SI-PFHA. Dual-channel separation system is integrated based on two superwetting membranes. Such separation system is competent for continuous light/heavy oil–water separation with high flux and high separation efficiency. Importantly, outstanding durability and reusability make this separation system a promising strategy for practical application in remediation of oily wastewater.
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SCIENTIA HORTICULTURAE
·
2018
·
IF 1.76
Huiyun Chen,Zhengxuan Sun,Huqing Yang
Indian jujube is perishable and has a relatively short shelf-life at room temperature. The effects of carnauba wax (CW) and CW containing glycerol monolaurate (CW-GML) coating on physico-chemical and qualitative attributes of jujube fruit are investigated during storage at 20 °C for 12 days. Compared to the control, both CW and CW-GML coatings reduce jujube weight loss, respiration rate, and ethylene production, maintain lower activities of polygalacturonase, pectin methylesterase and cellulase and delay the flesh softening. The two coatings also delay the change of skin color and ensure higher content of chlorophyll and ascorbic acid. However, the CW-GML coating most significantly inhibite the decay of jujube fruit and retains better sensory quality. After 12 d of storage at 20 °C, the decay index is only 44.3% of the control fruit, and 65.6% of the CW-coated jujube. These results confirm that CW-GML coating could be an effective means to contribute to the shelf-life extension and quality maintenance of jujube fruit stored at 20 °C.
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JOURNAL OF CHROMATOGRAPHY A
·
2020
·
IF 4.049
Tangjuan Zhao,Mengyan Zhang,Ling Ma,Li Ma,Hongmei Shi,Weijun Kang,Xiangdong Xu
A novel magnetic solid-phase extraction (MSPE) material (Fe 3 O 4 @SiO 2 -NH 2 -G2) had been prepared and employed for adsorption and analysis of seven quaternary ammonium compounds (QACs) in infant fruit and vegetable products coupled with high performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS). In this paper, Fe 3 O 4 @SiO 2 -NH 2 -G2 was synthesized based on Fe 3 O 4 @SiO 2 -NH 2 and dendrimer (G2) consisting of cyanuric chloride and imidazole . The morphology, configuration and magnetic behavior of the magnetic material were characterized by transmission electron microscopy (TEM), Fourier transform infrared spectrometry (FT-IR), X-ray diffraction (XRD), and vibrating sample magnetometer (VSM). Critical parameters affecting extraction efficiency, such as the adsorbent amount, sample pH, extraction time, the type of eluent, and desorption time, were optimized. The proposed method provided good linearity with the correlation coefficients ( R 2 ) of 0.9992-0.9999, low limits of detection (LODs) (0.05-0.50 μg kg −1 ) and limits of quantitation (LOQs) (0.20-2.00 μg kg −1 ). The satisfactory method recoveries in three spiked infant fruit and vegetable products samples were between 80.12% and 101.35% with the relative standard deviations (RSDs) less than 12.04%. In summary, the established method was an effective sample preparation method and showed good prospect for the analysis of QACs in complex matrices.
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高影响因子
ANALYTICAL SCIENCES
·
2020
·Vol. 36
·期 3
·353-360
·
IF 2.049
Sifan XU,Shuqi YE,Yunhui XU,Feifan LIU,Yushun ZHOU,Qian YANG,Hailong PENG,Hua XIONG,Zhong ZHANG
To achieve a rapid, sensitive, and economical method for the detection of ascorbic acid (AA) in the presence of Fe3+, a nitrogen and sulfur co-doped carbon dots (N,S-co-CDs) based fluorescence sensing system was developed. In this work, N,S-co-CDs were successfully synthesized via a one-step microwave-assisted method within 2.5 min using ammonium citrate and L-cysteine as precursors. The fluorescence of N,S-co-CDs was quenched (off ) by Fe3+ through a static-quenching mechanism. Subsequently, the fluorescence was recovered (on) after introducing AA into the quenched system, which was attributed to the reduction effect of AA for Fe3+. Therefore, a switch-on sensor (N,S-co-CDs/Fe3+ system) was developed for AA detection. Under optimal conditions, the limit of detection (LOD) of 2.31 μmol/L for AA was obtained over a linear range from 0 to 150 μmol/L. Furthermore, the proposed sensing method was successfully applied to detect AA in processed fruit juice with satisfactory results. The most important is that the sensor derived from a microwave-assisted method has simple and eco-friendly synthesis processes, is rapid, and has high detection efficiency. Therefore, such a switch-on sensor may be a promising candidate sensor for AA detection in processed fruit samples.
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Frontiers in Nutrition
·
2021
·
IF 6.576
Wang Ruimin,He Ruiping,Li Zhaohui,Wang Lu
Clausena indica (Datz.) Oliv fruit pericarps (CIOPs) is an important agro-industrial by-product rich in active components. In this article, the effects of traditional and green deep eutectic solvents (DESs) on the high-performance liquid chromatography (HPLC) characterization, antioxidant activities, and α-glucosidase-inhibitory activity of phenolic extracts from CIOPs were investigated for the first time. The results showed that ChCl-Gly and Bet-CA had higher extraction efficiency for the total phenolic content (TPC, 64.14–64.83 mg GAE/g DW) and total flavonoid content (TFC, 47.83–48.11 mg RE/g DW) compared with the traditional solvents (water, methanol, and ethyl acetate). LC-Q-Orbitrap-MS/MS was adopted to identify the phenolic compositions of the CIOPs extracts. HPLC-diode array detection (HPLC-DAD) results indicated that arbutin, (–)-epigallocatechin, chlorogenic acid, procyanidin B1, (+)-catechin, and (–)-epicatechin were the major components for all extracts, especially for deep eutectic solvents (DESs). In addition, ChCl-Xyl and ChCl-Gly extracts showed higher antioxidant activities against 2,2-diphenyl-1-picrylhydrazyl (DPPH•), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid (ABTS+•), ferric reducing antioxidant power (FRAP), reducing power (RP), and cupric ion reducing antioxidant capacity (CUPRAC) than extracts extracted by other solvents. A strong α-glucosidase-inhibiting activity (IC50, 156.25-291.11 μg/ml) was found in three DESs extracts. Furthermore, in silico analysis of the major phenolics in the CIOPs extracts was carried out to explore their interactions with α-glucosidase. Multivariate analysis was carried out to determine the key factors affecting the antioxidant activity and α-glucosidase-inhibiting activity. In short, DES can be taken as a promising solvent for valorization and recovery of bioactive compounds from agro-industrial by-products. The results verified that CIOPs can be used as a prospective source rich in bio-active compounds applied in the food and pharmacy industries.
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