SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2025
·
IF 4.6
Kai Wang,Yan-Ru Wang,Jun Zhao,Ren-Min Zhou,Xi-Feng Xu,Tong Wang,Zhong-Yuan Cheng
Hepatic ischemia-reperfusion (I/R) injury is a major clinical complication, and the precise monitoring of key inflammatory mediators like neutrophil elastase (NE) in vivo remains a significant challenge. To address this, we developed HDS-NE, a highly specific and activatable molecular probe based on a sulfur-substituted hemicyanine scaffold, engineered for the simultaneous detection of NE via fluorescence and photoacoustic imaging modalities. The probe demonstrated exceptional sensitivity and selectivity toward NE, enabling the real-time visualization of elevated NE activity in a murine model of hepatic I/R injury. Both in vivo and ex vivo imaging results confirmed a markedly enhanced signal in injured livers compared to controls, which was further validated by serum assays and histology. Crucially, this study represents the successful achievement of non-invasive, in vivo imaging of NE activity during hepatic I/R processes, offering a powerful tool for diagnosing and studying inflammatory liver diseases.
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PubMed
高影响因子
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
·
2025
·Vol. 122
·期 6
·e2417735122
·
IF 9.1
Wangshu Mou,Ria Khare,Joanna K. Polko,Isaiah Taylor,Juan Xu,Dawei Xue,Philip Benfey,Bram Van de Poel,Caren Chang,Joseph J. Kieber
In seed plants, the canonical role of 1-aminocyclopropane-1-carboxylic acid (ACC) is to serve as the precursor in the biosynthesis of the phytohormone ethylene, and indeed, ACC treatment is often used as a proxy for ethylene treatment. Increasing evidence suggests that ACC can also act independently of ethylene to regulate various aspects of plant growth and development. Here, we explore the effects of ACC on Arabidopsis thaliana root growth and the mechanisms by which it acts. ACC inhibits growth of the primary root in Arabidopsis seedlings when ethylene signaling is blocked, which becomes evident after 36 h of treatment with ACC. This reduced root growth is in part the result of suppressed cell proliferation in the root meristem resulting from altered expression of a key regulator of stem cell niche activity, WOX5. ACC also promotes lateral root (LR) development, in contrast to ethylene, which inhibits LR formation. Transcriptomic analysis of roots revealed no significant changes in gene expression after 45 min or 4 h of ACC treatment, but longer treatment times revealed a large number of differentially expressed genes, including the downregulation of the expression of a small group of phylogenetically related CLE peptides. Reduced expression of these group 1 CLEs in response to ACC leads to the activation of a transcription factor, LBD18, which promotes LR development. These results suggest that ACC acts to modulate multiple aspects of Arabidopsis root growth independently of ethylene via distinct transcriptional effects in the root meristem and LR precursor cells.
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产品关联
DOI
PubMed
高影响因子
KAOHSIUNG JOURNAL OF MEDICAL SCIENCES
·
2025
·
IF 3.1
Qing Su,Jia Chen
Steroid-induced osteonecrosis of the femoral head (SIONFH) is a debilitating orthopedic condition. This study investigated the mechanism of Tanshinone I (TsI) in SIONFH modulating apoptosis in SIONFH via the PI3K/AKT/mTOR pathway. A SIONFH rat model was treated with TsI and a PI3K activator. Bone mineral density (BMD), bone volume/total volume (BV/TV), trabecular number (Tb.N), trabecular thickness (Tb.Th), and trabecular separation (Tb.Sp) were determined by microCT. Empty lacunae count, Osteopontin, and apoptosis of the femoral head tissues were assessed. Levels of Bax, cleaved-caspase-3, Bcl-2, and AKT, PI3K, and mTOR phosphorylation in femoral head tissues were determined by Western blot. SIONFH rats exhibited decreased BMD, BV/TV, Tb.N, and Tb.Th, increased Tb.Sp, reduced Osteopontin-positive cells, increased empty lacunae rate, and TUNEL and Osteopontin co-positive cells, elevated Bax and cleaved-caspase-3 protein levels, and diminished Bcl-2 protein expression. TsI promoted osteogenesis, attenuated SIONFH, and reduced apoptosis in SIONFH rats. TsI inhibited AKT, PI3K, and mTOR phosphorylation levels in the femoral head tissues of SIONFH rats, thereby repressing the PI3K/AKT/mTOR pathway activation. Activating the PI3K/AKT/mTOR pathway partially reversed TsI's effect in rats. Collectively, TsI limited the PI3K/AKT/mTOR pathway activation to reduce osteocyte apoptosis in SIONFH rats, which provided potential therapeutic insights for SIONFH treatment.
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高影响因子
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
·
2021
·
IF 3.575
Dequan Kong,Baoying Xie,Yuhang Li,Yaping Xu
Palmitoylethanolamide (PEA) offers a strong protection against BBB disruption and neurological deficits after cerebral ischaemic/reperfusion (I/R) injury. To date, these BBB protective effects of PEA are mainly attributed to PPARα-mediated actions. However, whether PEA protects against BBB disruption through direct regulation of cytoskeletal microfilaments remains unknown. Here, we identified PEA as a Rho-associated protein kinase (ROCK2) inhibitor (IC 50 = 38.4 ± 4.8 μM). In vitro data suggested that PEA reduced the activation of ROCK/MLC signaling and stress fiber formation within microvascular endothelial cells (ECs) after oxygen-glucose deprivation (OGD), and consequently attenuated early (0–4 h) EC barrier disruption. These actions of PEA could not be blocked by the PPARα antagonist GW6471. In summary, the present study described a previously unexplored role of PEA as a ROCK2 inhibitor, and propose a PPARα-independent mechanism for pharmacological effects of PEA.
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EXPERIMENTAL NEUROLOGY
·
2020
·
IF 4.691
Shuai Zhang,Mei-Ling Yan,Lin Yang,Xiao-Bin An,Hong-Mei Zhao,Sheng-Nan Xia,Zhuo Jin,Si-Yu Huang,Yang Qu,Jing Ai
Chronic cerebral hypoperfusion (CCH) promotes the development of Alzheimer's pathology. However, whether and how CCH impairs the synaptic vesicle trafficking is still unclear. In the present study, we found that the hippocampal glutamatergic vesicle trafficking was impaired as indicated by a significant shortened delayed response enhancement (DRE) phase in CA3-CA1 circuit and decreased synapsin I in CCH rats suffering from bilateral common carotid artery occlusion (2VO). Further study showed an upregulated miR-153 in the hippocampus of 2VO rats. In vitro, overexpression of miR-153 downregulated synapsin I by binding the 3′UTRs of SYN1 mRNAs, which was prevented by its antisense AMO-153 and miRNA-masking antisense oligodeoxynucleotides ( SYN1-ODN ). In vivo , the upregulation of miR-153 elicited similar reduced DRE phase and synapsin I deficiency as CCH. Furthermore, miR-153 knockdown rescued the downregulated synapsin I and shortened DRE phase in 2VO rats. Our results demonstrate that CCH impairs hippocampal glutamatergic vesicle trafficking by upregulating miR-153 , which suppresses the expression of synapsin I at the post-transcriptional level. These results will provide important references for drug research and treatment of vascular dementia .
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RUSSIAN JOURNAL OF INORGANIC CHEMISTRY
·
2020
·Vol. 65
·期 8
·1186-1195
·
IF 0.94
Xue-Wen Liu,Liu Ning-Yi,Deng Yuan-Qing,Wang Shan,Liu Ting
Abstract Two ruthenium complexes containing nitro-substituted ligand, [Ru(bpy) 2 (hnpip)](PF 6 ) 2 ( 1 ) and [Ru(phen) 2 (hnpip)](PF 6 ) 2 ( 2 ) (bpy = 2,2'-bipyridine; phen = 1,10-phenanthroline; hnpip = 2-(3-hydroxyl- 4-nitrophenyl)imidazo[4,5- f ][1,10-phenanthroline] were synthesized and characterized. DNA binding behaviors were studied by luminescent titration, UV-vis titration, FRET (FRET = Fluorescence resonance energy transfer) and molecular docking experiments. Complex 2 exhibited “light switch” effect upon addition of G-quadruplex DNA and displayed fluorescent selectivity towards G-quadruplex DNA over other DNAs in the presence of K 4 [Fe(CN) 6 ]. Fluorescence experiments, FRET experiments and molecular docking results indicated that the selectivity may be attributed to protection of nitro group from water, DNA affinity and the difference in DNA structures. Furthermore, both complexes were found to cleave DNA under irradiation by the formation of singlet oxygen. DNA topoisomerase inhibition experiments indicated that complex 2 exhibited higher topoisomerase I inhibition activity (IC 50 = 18 μM) than complex 1 (IC 50 = 50 μM). Docking studies revealed that complex 2 stabilized Top1cc complex via π–π interaction and the formation of hydrogen bond.
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Journal of Functional Foods
·
2020
·
IF 3.701
Zhenbang Xiao,Shengtao Yang,Jiali Chen,Chengyong Li,Chunxia Zhou,Pengzhi Hong,Shengli Sun,Zhong-Ji Qian
Ultraviolet radiation B cause photoaging of the skin by enhancing the high expression of matrix metalloproteinases (MMPs) to degrade collagen and inhibit the synthesis of procollagen. Trehalose (TRE), a widely occurring natural disaccharide, has been shown to play a cytoprotective role in many pathological processes. However, few studies have evaluated whether it has anti-photoaging potential. In this study, we investigated the anti-photoaging and mechanism of action of TRE using Human immortalized keratinocytes (HaCaT) cells. The results indicated that TRE pretreatment effectively scavenges reactive oxygen species (ROS) accumulation induced by UVB and increases the content of certain endogenous antioxidant factors in HaCaT cells. TRE suppressed UVB-induced MMP expression by blocking mitogen-activated protein kinases (MAPKs), activator protein 1 (AP-1) and NF-κB signaling. TRE treatment increased procollagen I synthesis via activating transforming growth factor-β (TGF-β)/Smad pathway and demonstrated that TRE may be a potential natural disaccharide for photoaging therapy in the future.
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LETTERS IN ORGANIC CHEMISTRY
·
2024
·Vol. 22
·期 1
·56-60
·
IF 0.7
Xin-Yu Lin,Guo-Jun Chen,Su-Zhe Wu,Ke-Xuan Xu,Xiao-Tian Zhang,Qi Feng
4-Hydroxyquinoline-2(1H)-ones exhibit multiple biological activities, and studying their synthetic methods is of great significance. In the presence of tetramethylguanidine and silver nitrate, 4-hydroxyquinoline-2(1H)-ones were efficiently synthesized from 2-ethynylanilines and carbon dioxide under atmospheric pressure. The reaction conditions, such as types of silver catalysts and reaction solvents, were optimized, and the applicability of the substrate was preliminarily investigated. This method provides an alternative pathway for the synthesis of 4-hydroxyquinoline-2(1H)-ones and the conversion and utilization of carbon dioxide.
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高影响因子
CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
·
2023
·Vol. 39
·期 4
·654-659
·
IF 3.1
Zhu Xia,An Jiakun,Chen Gaoyu,Xing Chenyu,Bao Jianchun,Xu Xiangxing
Perovskite single crystals are prominent semiconductor materials due to their high carrier mobility, long carrier diffusion length, and large absorption coefficient. They can be used as optoelectronic functional materials for narrowband or broadband photodetectors. In this report, the photodetection band tunability was achieved by doping MAPbX 3 (MA=methylammonium, X=Cl/Br/I) perovskite single crystals with sub-nano carbon dots (MAPbX 3 -C). The sub-nano size and good dispersibility of the carbon dots ensured controllable doping. The absorption of the sub-nano carbon dots and trap states in the MAPbX 3 -C single crystals resulted in the special responsivity spectrum features.
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Desalination and Water Treatment
·
2024
·
IF 1
Song Deng,Jingrong Huang,Meng Guo,Xueni Sun,Xiuxiu Ren,Jing Zhong,Rong Xu
Novel p-phenylenediamine-bridged organosilica (PPDA-BOS-T) materials were synthesized via the co-condensation reaction assisted by tetraethyl orthosilicate (TEOS). These amino-functionalized organosilicas were characterized by Fourier transform infrared spectrometer, N 2 sorption, scanning electron microscopy, and transmission electron microscopy. The addition of TEOS played a positive role in the construction of the porous network structure of the bridged organosilicas. The saturation adsorption capacity for Ag(I) of the PPDA-BOS-T could reach 82.88 mg g –1 . The conformity to the Freundlich model indicated multilayer adsorption with active sites. Furthermore, the adsorption was followed well by the pseudo-second-order model, and the intraparticle diffusion was proved to be the rate-limiting step during the adsorption of Ag(I). Finally, the thermodynamic parameters including the change of free energy (ΔG), enthalpy (ΔH), and entropy (ΔS) of adsorption were calculated.
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JOURNAL OF POLYMER RESEARCH
·
2013
·Vol. 21
·期 1
·1-13
·
IF 2.019
Li Xing-Jian,Sun Dao-Xing,Zhang Yi-Heng
The Cu(I)-mediated azide–alkyne cycloaddition reaction, “click” chemistry, has been used to functionalize the waterborne polyurethane, by which a novel fluorinated waterborne polyurethane (FWPU) was synthesized. FWPU was confirmed by FT-IR and 1 H NMR. The structure and properties of FWPU were characterized by water contact angle, water absorption, surface energy, scanning electron microscope (SEM) equipped with energy dispersive X-ray spectrometry (EDS), thermogravimetric analysis (TGA) and X-ray diffraction (XRD), etc. From the research of the surface properties, it was found that the modified materials were endowed with a remarkable hydrophobicity and stain resistance. SEM results showed that the micro-phase separation of FWPU became increasingly apparent with the rise of fluorinated side chains in polyurethane backbone. XRD measurements reflected that the crystallinity of FWPU tended to decrease firstly and then increase with the change of fluorine content. TGA results demonstrated that FWPU possessed flame retardant performance to a certain extent due to the incorporated alkyne groups in the FWPU to improve the final char strongly.
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FOOD CONTROL
·
2022
·
IF 6.652
Xuanyu Sha,Siqingaowa Han,Guoqiang Fang,Nan Li,Dianyang Lin,Wuliji Hasi
In this work, a facile and effective method for assembly and transfer nanoparticles on thin layer chromatography (TLC) plate was proposed and used to combined with layer chromatography-surface-enhanced Raman spectroscopy (TLC-SERS) strategy for detection of Rhodamine B and Sudan I. First, Monodispersed Ag NCs with broadly tunable diameters (from 41 to 67 nm) and 7.25 × 10 5 maximum actual enhancement factor (AEF) were successfully prepared and systematically investigated for its optic performance use in TLC-SERS. Au@Ag NCs were monitored by TEM, SEM and UV–Vis spectrometer. The optical properties of Ag NCs were simulated and the experimental results showed good consistency with simulation results of absorption spectra. Through three phases interfacial technique and a simple transfer method, nanostructures were assembled on the TLC plate. Moreover, the semi-quantitative analysis of Rhodamine B and Sudan I in the range of 1–200 ppm was performed based on the assembled TLC plate with the limit of detection as low as 1 ppm. In addition, the practical feasibility of this strategy was verified by the detection of Sudan I and Rhodamine B in chili oil. In general, this assembled TLC-SERS strategy builds a connection for the application of nanomaterials with rich morphology in TLC-SERS, which has a great application prospect and practical application value.
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