JOURNAL OF MATERIALS SCIENCE
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2022
·Vol. 57
·期 13
·6943-6952
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IF 4.682
Zhang Yongjun,Zhao Xiaoyu,Zhu Qi,Cheng Mingyu,Xu Wei,Wang Yaxin,Zhu Aonan,Gao Renxian,Zhang Jian,Zhang Xiaolong
As an important marker of Hepatocellular carcinoma (HCC), AFP-L3 has attracted attention. In this study, the surface-plasmon-assisted Ag nanoparticles symmetrical growth technology under magnetic field is used to fabricate the six-nanohole nanojar array containing Ag nanoparticles as the sensing substrate. The displacement of SERS signal of the optimized structure is used to detect HCC marker AFP-L3. This method has good stability, high accuracy and has great potential in the early diagnosis of HCC.
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OPTICS EXPRESS
·
2022
·Vol. 30
·期 21
·37795-37814
·
IF 3.833
Chuansong Chen,Chuansong Chen,Wenjie Zhang,Wenjie Zhang,Pengyi Duan,Wenying Liu,Muhammad Shafi,Muhammad Shafi,Xiaoxuan Hu,Can Zhang,Chao Zhang,Baoyuan Man,Baoyuan Man,Mei Liu
Improving the photo-induced charge transfer (PICT) efficiency by adjusting the energy levels difference between adsorbed probe molecules and substrate materials is a key factor for boosting the surface enhanced Raman scattering (SERS) based on the chemical mechanism (CM). Herein, a new route to improve the SERS activity of two-dimensional (2D) selenium and tin compounds (SnSex, 1 ≤ x ≤ 2) by the hybrid phase materials is researched. The physical properties and the energy band structure of SnSex were analyzed. The enhanced SERS activity of 2D SnSex can be attribute to the coupling of the PICT resonance caused by the defect energy levels induced by Se vacancy and the molecular resonance Raman scattering (RRS). This established a relationship between the physical properties and SERS activity of 2D layered materials. The resonance probe molecule, rhodamine (R6G), which is used to detect the SERS performance of SnSex nanosheets. The enhancement factor (EF) of R6G on the optimized SnSe1.35 nanosheets can be as high as 2.6 × 106, with a detection limit of 10−10 M. The SERS result of the environmental pollution, thiram, shows that the SnSex nanosheets have a practical application in trace SERS detection, without the participation of metal particles. These results demonstrate that, through hybrid phase materials, the SERS sensitivity of 2D layered nanomaterials can be improved. It provides a kind of foreground non-metal SERS substrate in monitoring or detecting and provide a deep insight into the chemical SERS mechanism based on 2D layered materials.
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APPLIED SURFACE SCIENCE
·
2022
·
IF 7.392
Shengyuan Yao,Yan Lv,Qing Wang,Jinhui Yang,Hongmei Li,Ningning Gao,Furu Zhong,Jihong Fu,Jun Tang,Tao Wang,Dianzeng Jia
As a new kind of 2D carbon allotrope, graphdiyne (GDY) has the characteristics of uniform pores and large specific surface area due to its unique electronic space arrangement structure. Therefore, we fabricated a novel gold nanoparticles (Au NPs)/GDY/carbon cloth (CC) SERS substrate by a simple electrodeposition process using GDY nanowall wrapping the CC as support. The effects of electrodeposition conditions on the microstructure and SERS activity of Au NPs/GDY/CC substrate were studied. Under the condition of optimizing deposition time and deposition voltage, the prepared Au NPs/GDY/CC substrate can detect 10 -13 M with rhodamine 6G (R6G) as a probe molecule. The maximum enhancement factor (EF) is 2.2 × 10 12 , and the concentration of R6G is logarithmically correlated with the intensity of the SERS band with a linear correlation coefficient (R 2 ) of 0.97036. These indicate that the Au NPs/GDY/CC substrate has good sensitivity. The prepared Au NPs/GDY/CC SERS substrate was applied to the detection of organic dyes of malachite green (MG) and methyl violet (MV) in water, and the detection limits of both can reach 10 -9 M. This highly sensitive and high-performance SERS substrate has broad application prospects in the detection of organic dyes.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2022
·
IF 4.831
Hongji Li,Junfu Wang,Xiyue Wang,Haochen Yu,Linjing Ji,Tianyu Zhou,Chunbo Liu,Guangbo Che,Dandan Wang
In this test, the eggshell membrane (ESM) is selected as the support membrane for the biocompatibility and anchors CNTs on the surface to increase the mechanical properties. Then Ag NPs are decorated on CNTs-ESM substrate as SERS substrate by twice in-situ reduction. Finally, a layer of imprinted polymers is coated on the surface of the substrate to synthesize the imprinted membrane for selective detection of spiramycin. It is exhibited from the characteristic results that the CNTs significantly increase the mechanical properties and the detection sensitivity, simultaneously. When the concentration of SP changes between 10 -6 ∼ 10 -11 M, there is a linear relationship between SERS intensity and SP concentration. The detection limit is 10 -11 M, and the correlation coefficient R 2 is 0.9864. The SERS imprinted membrane can be applied into the detection of antibiotics in practical sample, which broadens the research field of antibiotics detection.
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高影响因子
ANALYTICA CHIMICA ACTA
·
2022
·
IF 6.911
Wenrui Ma,Lulu Liu,Xu Zhang,Xingfei Liu,Yi Xu,Shunbo Li,Muling Zeng
Developing sensitive and miniaturized biosensors for the detection of microRNAs (miRNAs) is highly desirable due to their association with early cancer diagnosis and prognosis. Here, a new microfluidic-based biosensor, combined with multifunctional nanosurface and DSN-assisted target recycle amplification strategy, is designed for the detection of miRNA-21. The design of nanosurface includes gold nanoparticles on porous anodic aluminum oxide (AAO) for surface enhanced Raman scattering (SERS) substrate, Au MBA @Ag core-shell nanoparticles for SERS nanotags and single-stranded DNA (ssDNA) in between for miRNA capture and nanotags immobilization. When the target miRNA is present near the nanosurface, it will be captured by ssDNA via hybridization reaction. Then, triggered by the DSN-assisted target recycle process, the freshly formed DNA/miRNA heteroduplexes are cleaved by DSN enzyme into DNA fragments and single-strand miRNA. The SERS nanotags are also dissociated from the nanosurface, leading to decrease of SERS signal. The cleaved target miRNA can be captured and SERS nanotags are released again in the next cycle, resulting in amplification of detection signal. To improve the accuracy of this biosensor, the functionalized AAO membrane is subdivided into two groups - AAO/Au array linked with encoded core-shell SERS nanotags acting as a reactor and primary detector and AAO/ [email protected] array serving as a collector and secondary detector for the dissociative SERS nanotags from the reactor. The decrease of SERS signal in primary detector and increase of signal in secondary detector ensures the accuracy and it is called dual-SERS detection strategy. The detection of miRNA-21 can be achieved with only 30 μL sample and 10 μL enzyme and a wide linear range of 10 fM∼10 nM is obtained. In addition, the microfluidic dual-SERS detection strategy can greatly reduce the possibility of false positive or false negative in single detection mode and it can be applied to the simultaneous detection of multiple miRNAs via integrating different probes.
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高影响因子
MOLECULES
·
2022
·Vol. 27
·期 12
·3939
·
IF 4.927
Yazhou Qin,Fan Mo,Sen Yao,Yuanzhao Wu,Yingsheng He,Weixuan Yao
Porous noble metal nanomaterials have attracted extensive attention due to their high specific surface area and surface plasmon resonance effect. However, it is difficult to form porous structures due to the high mobility and low reduction potential of noble metal precursors. In this article, we developed a facile method for preparing porous Ag with a controllable structure at room temperature. Two kinds of Ag crystals with different porous structures were successfully prepared by using AgCl cubes as sacrificial templates. Through the galvanic replacement reaction of Zn and AgCl, Ag crystals with a sponge-like porous structure were successfully prepared. Additionally, using NaBH4as the reducing agent, we prepared granular porous Ag cubes by optimizing the amount of reducing agent. Both the sponge-like and granular porous Ag cubes have clean and accessible surfaces. In addition, we used the prepared two porous Ag cubes as substrate materials for SERS detection of five kinds of methamphetamine analogs. The experimental results show that the enhancement effect of granular porous Ag is better than that of sponge-like porous Ag. Furthermore, we probed the hot spot distribution of granular porous Ag by Raman mapping. By using granular porous Ag as the substrate material, we have achieved trace detection of 5 kinds of methamphetamine analogs including Ephedrine, Amphetamine, N-Methyl-1-(benzofuran-5-yl)propan-2-amine (5-MAPB), N-Methyl-1-(4-methoxyphenyl)propan-2-amine (PMMA) and N-Methyl-1-(4-fluorophenyl)propan-2-amine (4-FMA). Furthermore, to achieve qualitative differentiation of analogs with similar structures we performed density functional theoretical (DFT) calculations on the Raman spectra of the above analogs. The DFT calculations provided the vibrational frequencies, Raman activities, and normal mode assignment for each analog, enabling the qualitative differentiation of the above analogs.Keywords:Ag crystal;methamphetamine;SERS;porous structure
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Food Analytical Methods
·
2022
·Vol. 15
·期 12
·3468-3478
·
IF 3.498
Li Junkui,Zuo Min,Zhang Wen,Zou Xiaobo,Sun Zongbao
The use of gutter oil in cooking, which poses a serious threat to human health, has attracted increasing attention in recent years. Capsaicin, existing in chili peppers, is used to identify gutter oil. Here, in order to detect capsaicin fast and accurately for gutter oil discrimination, we introduce a hybrid method to detect capsaicin based on a diazo coupling reaction and surface-enhanced Raman scattering (SERS) technology. A diazo coupling reaction is applied to the composite of p-aminothiophenol (4-ATP) and capsaicin, and the product, azo dye, is adopted as the SERS probe. Gold nanorods (Au NRs) are prepared using the seed-mediated growth method and utilized as the SERS substrate. The Au–S bond between the azo dye and the Au NRs has a powerful ability to spontaneously capture target molecules on the sensing interface. The production of the diazo coupling reaction is proven with an ultraviolet–visible (UV–vis) absorption spectrum. As-synthesized Au NRs are characterized with a UV–vis absorption spectrum, transmission electron microscope, and SERS. Significantly improved SERS peaks were observed at 1114 and 1392 cm −1 , showing this method can be used to detect capsaicin and identify gutter oil. In a broad range of 10 −11 ~ 10 −4 M, the log-linear relationship between SERS intensity and capsaicin concentration is well established, with a detection limit of 3.24 × 10 −12 M.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2021
·
IF 4.098
Wenxian Wei,Dan Yu,Yu Du,Yicheng Ding,Qingli Huang
Au-Ag alloy nanoparticles (NPs) with controllable size and composition were synthesized by a facile, one-pot hydrothermal method. Various characterization techniques including TEM, UV–vis, EDX, HAADF-STEM and XPS were used to discuss the influencing factors for the size and composition of Au-Ag alloy NPs. It is obvious that the size and composition of Au-Ag alloy NPs could be adjusted by the experimental parameters. Catalytic and SERS performance of the Au-Ag alloy NPs were further investigated. Ideal catalytic and SERS performance could be also obtained via optimizing the size and composition of Au-Ag alloy. This work is of importance in theory research and practical application of the noble metal nanocomposites.
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高影响因子
NEW JOURNAL OF CHEMISTRY
·
2021
·Vol. 45
·期 41
·19593-19604
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IF 3.591
Yaoyu Tan,Huan Jiang,Baihui Wang,Xia Zhang
Surface-enhanced Raman scattering (SERS) is an ultrasensitive fingerprint spectroscopy technique. The combined application of SERS and nanozymes has shown excellent prospects in the field of biomedical diagnosis. In the present study, polyaniline@MoS2@Fe3O4@Au (PANI@MoS2@Fe3O4@Au) nanozymes were successfully prepared using hydrothermal reactions. PANI@MoS2@Fe3O4@Au nanozymes have not only dramatically enhanced the peroxidase-like activity but also significantly enhanced the SERS performance. We also studied the effect of different concentrations of HAuCl4·4H2O on the SERS activity of nanozymes and found that PANI@MoS2@Fe3O4@Au nanozymes prepared using 2 mg mL−1 HAuCl4·4H2O solution exhibited the best SERS performance. We used SERS to study the enzyme kinetics of the nanozymes, and the results showed that the nanozymes have a strong affinity for H2O2 and TMB. Finally, we combined PANI@MoS2@Fe3O4@Au nanozymes and glucose oxidase (GOx) to establish a SERS glucose biosensor, which has the advantages of ultrasensitivity, high-precision, and a wide linear response range. The linear response range of glucose is 10−11–10−3 M, and the detection limit is 10−12 M. More importantly, we have successfully determined the glucose content in human serum samples with satisfactory accuracy. This work provides an essential new strategy for designing composite nanozymes for biocatalysis and provides a new idea for constructing high-sensitivity SERS biosensors.
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TALANTA
·
2021
·
IF 6.057
Guanli Fan,Xia Li,Shuling Xu,Caifeng Dai,Qingwang Xue,Huaisheng Wang
Simple and sensitive detection of telomerase activity is of vital importance for both early diagnosis and therapy of malignant tumors. Inspired by DNA-biobarcode amplification reported by Chad A. Mirkin, we developed a facile DNA-biobarcode-like SERS-based copper-mediated signal amplification strategy for sensitive detection of telomerase activity. In this strategy, a duplex DNA constructed by hybridization of a copper oxide nanoparticle (CuO NP)-labeled reporting sequence (RS) with the telomerase primer sequence (TS) is ingeniously designed, and anchored on the magnetic bead (MB) to build the CuO NPs-encoded magnetic bead (MB-CuO NPs) detection probe. Upon selective sensing of telomerase, telomerase elongation reaction and structure change of TS products make the CuO NP-RS displace and separate from MB. The separated CuO NPs are dissolved into a mass of Cu 2+ , which prompt monodisperse dopamine-functionalized AgNPs (D-AgNPs) signal probe into aggregation, resulting in color changes and significantly enhancing of SERS signal. The SERS signal increases with the increase of Cu 2+ , which is directly proportional to the telomerase. Benefiting from the transformation of CuO NP to Cu 2+ with a high amplification effect, this strategy could realize the telomerase activity measurement down to 3 HeLa cells and a dynamic range of 10–10000 cells. It shows a significant improvement of sensitivity without need for other enzymes and elaborate design, which escapes from the complicated manipulations and design in polymerase chain reaction (PCR) and DNA amplification techniques. Moreover, with this strategy, telomerase activities of different cell lines and telomerase inhibitors screening were successfully performed. Significantly, it can also be utilized for visual detection of telomerase, which validates the potential on-site application and its application as point-of-care testing (POCT) for efficient monitoring. Given the high-performance for telomerase analysis, the strategy has a promising application in biological detection and clinical diagnosis, as well as point-of-care tests.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Ziluo Su,Hongmei Liu,Ying Chen,Chenjie Gu,Guodong Wei,Tao Jiang
Aiming to augment the reliability and efficiency of surface enhanced Raman scattering (SERS)-active immunoprobe, sophisticated strategies that are able to avoid the dissociation of Raman molecules and noble metal nanoparticles (NPs) in practice have drawn a considerable attention. This study confirmed that Ag@polystyrene-block-poly(acrylic acid)@Ag core-shell nanostructures possessed superior stability compared with bare Ag NPs in phosphate buffer saline solution and human serum. Combined with a unique paper-based immunosubstrate with a satisfying enhancement factor of 9.71 × 10 5 , a robust sandwich immunostructure was constructed and conducted to monitor carbohydrate antigen 19-9 (CA19-9), presenting a low limit of detection of about 10 −4 U/mL. With these exceptional features, such a proposed protocol was finally utilized to detect CA19-9 in several clinical human sera, which shown much higher sensitivity than the traditional chemiluminescence analysis.
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CHEMICAL PHYSICS LETTERS
·
2021
·
IF 2.328
Xufeng Hao,Weixuan Liu,Yajun Zhang,Weijun Kang,Lingmei Niu,Lianfeng Ai
Chlorpromazine hydrochloride (CPZ) is a phenothiazine antipsychotic drug, which can effectively relieve the symptoms of chronic or severe mental disorders and reduce the manic phase of manic depression. However, excessive CPZ (more than 2 g per day) can cause serious harm to the health of the body, such as cholestatic jaundice, leukopenia, orthostatic hypotension, extrapyramidal effects and contact dermatitis. Therefore, a simple, fast, and accurate method to detect and identify CPZ is very important, especially for the detection of biological samples. In this study, based on the principle of inorganic salt inducing nanoparticle agglomeration, we synthesized silver colloids (AgNPs) with plasmon resonance effect. And we introduced inorganic salt MgBr 2 to induce self-limiting aggregation of AgNPs, resulting in a large number of “hot spots” in the nanoparticle gap. Thereby significantly enhancing the SERS intensity. The surface-enhanced Raman spectroscopy (SERS) method proposed in this paper can achieve real-time rapid detection of chlorpromazine hydrochloride in the field, with a linear range of 0.1–100 ppm (R 2 = 0.992). The calculated detection limit was 0.048 ppm. This strategy enables high stability and good repeatability of the SERS spectra. The application of established method can achieve the rapid and accurate determination of CPZ in pig urine. The recovery of standard addition was 87.50–107.70% and the RSD was between 2.87% and 7.42%. Therefore, the SERS detection technology in this study provides a new insight for the rapid, effective and accurate determination and identification of CPZ in pig urine.
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