Cell and Bioscience
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2023
·Vol. 13
·期 1
·1-15
·
IF 7.5
Yu Juan,Yu Nianye,Tian Yao,Fang Yifan,An Bin,Feng Guihai,Wu Jun,Wang Liu,Hao Jie,Wang Liqiang,Zhou Qi,Li Wei,Wang Yukai,Hu Baoyang
Background Research on human pluripotent stem cells (hPSCs) has shown tremendous progress in cell-based regenerative medicine. Corneal endothelial dysfunction is associated with the loss and degeneration of corneal endothelial cells (CECs), rendering cell replacement a promising therapeutic strategy. However, comprehensive preclinical assessments of hPSC-derived CECs for this cell therapy remain a challenge. Results Here we defined an adapted differentiation protocol to generate induced corneal endothelial cells (iCECs) consistently and efficiently from clinical-grade human embryonic stem cells (hESCs) with xeno-free medium and manufactured cryopreserved iCECs. Cells express high levels of typical CECs markers and exhibit transendothelial potential properties in vitro typical of iCECs. After rigorous quality control measures, cells meeting all release criteria were available for in vivo studies. We found that there was no overgrowth or tumorigenicity of grafts in immunodeficient mice. After grafting into rabbit models, the surviving iCECs ameliorated edema and recovered corneal opacity. Conclusions Our work provides an efficient approach for generating iCECs and demonstrates the safety and efficacy of iCECs in disease modeling. Therefore, clinical-grade iCECs are a reliable source for future clinical treatment of corneal endothelial dysfunction.
研究领域:
再生医学
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干细胞生物学
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眼科
·
临床前研究
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DOI
PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2023
·Vol. 34
·期 20
·1-12
·
IF 2.8
Chen Yongzhi,Wen Xu,Zhang Xiaohong,Yang Chao,Wang Lihua,Zhou Lingfeng,Li Zuohong,Deng Haobo,Li Jian
The recycled graphite (RG) and a new type of recycled graphite with multi-layer coating structure (RGC) were prepared by using spent graphite and asphalt. The electrochemical properties and coatings of RG and RGC were also investigated. The low graphitization degree of hard carbon and soft carbon was beneficial to improve the electrochemical properties of regenerated graphite, which was verified by first principles. The unique structure (soft carbon/ hard carbon/ graphite) limited the volume expansion of hard carbon and graphite when lithium ions were embedded or extruded, which improved the rate capability and cycling performance of the battery. Compared with spent graphite (SG) and recycled graphite (RG), the first charge-specific capacity of RGC at 0.1 C was 349.17 mAh·g − 1 , and the coulombic efficiency was 82.47%. Impressive reversible capacity was 263.38 mAh·g − 1 after 130 cycles at 0.5 C and discharge capacity of 188 mAh·g − 1 at 1 C. Due to the pre-lithiation of RGC, the first coulomb efficiency (99.49%) of the full battery (18,650) was higher than that of commercial graphite, and the energy conservation rate was 65.6% after 2000 cycles. This study provides a simple and effective scheme, and realizes the clean recovery and high value commercial application of spent graphite.
研究领域:
材料科学
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能源存储
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DOI
高影响因子
Nature Communications
·
2021
·Vol. 12
·期 1
·1-10
·
IF 14.919
Shen Xing,Zhou Quan,Han Miao,Qi Xingguo,Li Bo,Zhang Qiangqiang,Zhao Junmei,Yang Chao,Liu Huizhou,Hu Yong-Sheng
Na-ion batteries have been considered promising candidates for stationary energy storage. However, their wide application is hindered by issues such as high cost and insufficient electrochemical performance, particularly for cathode materials. Here, we report a solvent-free mechanochemical protocol for the in-situ fabrication of sodium vanadium fluorophosphates. Benefiting from the nano-crystallization features and extra Na-storage sites achieved in the synthesis process, the as-prepared carbon-coated Na 3 (VOPO 4 ) 2 F nanocomposite exhibits capacity of 142 mAh g −1 at 0.1C, higher than its theoretical capacity (130 mAh g −1 ). Moreover, a scaled synthesis with 2 kg of product was conducted and 26650-prototype cells were demonstrated to proof the electrochemical performance. We expect our findings to mark an important step in the industrial application of sodium vanadium fluorophosphates for Na-ion batteries.
研究领域:
材料科学
·
电化学
·
储能技术
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DOI
PubMed
高影响因子
Nature Communications
·
2023
·Vol. 14
·期 1
·1-14
·
IF 16.6
Yang Ye,Yang Yaozhang,Liu Dingyuan,Wang Yuanyuan,Lu Minqiao,Zhang Qi,Huang Jiqing,Li Yongchuan,Ma Teng,Yan Fei,Zheng Hairong
Acoustic tweezers can control target movement through the momentum interaction between an acoustic wave and an object. This technology has advantages over optical tweezers for in-vivo cell manipulation due to its high tissue penetrability and strong acoustic radiation force. However, normal cells are difficult to acoustically manipulate because of their small size and the similarity between their acoustic impedance and that of the medium. In this study, we use the heterologous expression of gene clusters to generate genetically engineered bacteria that can produce numerous sub-micron gas vesicles in the bacterial cytoplasm. We show that the presence of the gas vesicles significantly enhances the acoustic sensitivity of the engineering bacteria, which can be manipulated by ultrasound. We find that by employing phased-array-based acoustic tweezers, the engineering bacteria can be trapped into clusters and manipulated in vitro and in vivo via electronically steered acoustic beams, enabling the counter flow or on-demand flow of these bacteria in the vasculature of live mice. Furthermore, we demonstrate that the aggregation efficiency of engineering bacteria in a tumour is improved by utilizing this technology. This study provides a platform for the in-vivo manipulation of live cells, which will promote the progress of cell-based biomedical applications.
研究领域:
生物医学工程
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基因工程
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超声技术
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细胞生物学
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DOI
PubMed
高影响因子
AAPS PHARMSCITECH
·
2021
·Vol. 22
·期 1
·1-10
·
IF 3.246
Zhao Yunchun,Zheng Haili,Wang Xiaorong,Zheng Xiaoling,Zheng Yongquan,Chen Yue,Fei Weidong,Zhu Jiahuan,Wang Wenxi,Zheng Caihong
Novel cationic lipid-based liposomes prepared using an amphiphilic cationic lipid material, N , N -dimethyl-( N ′, N ′-di-stearoyl-1-ethyl)1,3-diaminopropane (DMSP), have been proposed to enhance the transfection of nucleic acids. Herein, we designed and investigated liposomes prepared using DMSP, soybean phosphatidylcholine, and cholesterol. This novel gene vector has high gene loading capabilities and excellent protection against nuclease degradation. An in vitro study showed that the liposomes had lower toxicity and superior cellular uptake and transfection efficiency compared with Lipofectamine 2000. An endosomal escape study revealed that the liposomes demonstrated high endosomal escape and released their genetic payload in the cytoplasm efficiently. Mechanistic studies indicated that the liposome/nucleic acid complexes entered cells through energy-dependent endocytosis that was mediated by fossa proteins. These results suggest that such cationic lipid-based liposome vectors have potential for clinical gene delivery.
研究领域:
药剂学
·
分子生物学
·
生物技术
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DOI
PubMed
高影响因子
BMC Chemistry
·
2021
·Vol. 15
·期 1
·1-10
·
IF 2.61
Huirong Zhang,huina Zhang,Nifeng Chen,Li Li
Background Eucommia ulmoides seed oil is a functional health oil with a high content of unsaturated fatty acids. However, excessively high content of unsaturated fatty acids can cause E. ulmoides seed oil to easily spoil. Microencapsulation technology can effectively encapsulate substances, thereby prolonging the spoilage time of oil products. Methods In the present study, E. ulmoides seed oil from different manufacturers were analyzed by Agilent 7890B-5977A gas chromatography – mass spectrometry. Encapsulation efficiency, yield rate, and scanning electron microscopy results between microcapsules prepared use different wall materials and different methods (spray drying and complex coagulation) were compared to determine the best preparation process for microcapsules. The Wantong 892 professional oil oxidation stability tester was used to measure the induced oxidation time of the E. ulmoides seed oil and microcapsules. Conclusion E. ulmoides seed oil comprises > 80% unsaturated fatty acids with a high α-linolenic acid content, followed by linoleic acid. The most promising combination was chitosan:gum arabic at 1:8 as the wall material and complex coagulation. The best preparation had a wall material concentration, stirring speed, aggregation pH, and core-to-wall ratio of 2.5%, 500 rpm, 4.2, and 1:4, respectively. Microcapsules prepared under these conditions exhibited higher yield and encapsulation efficiency (94.0% and 73.3%, respectively). The induced oxidation time of the E. ulmoides seed oil and microcapsules were 3.8 h and 13.9 h, respectively, indicating that microencapsulation can increase the oxidation induction time of this oil.
研究领域:
食品科学
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化学工程
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制药技术
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DOI
PubMed
高影响因子
TRANSITION METAL CHEMISTRY
·
2020
·Vol. 45
·期 4
·253-266
·
IF 1.366
An Si-Ying,Su Yuan-Yuan,Qi Xin,Zhang Rong-Lan,Ma Yan-Ling,Zhao Jian-She
Abstract A series of Zn(II)-based complexes as photoinduced carbon-monoxide-releasing molecules (photoCORMs), viz. ( L )Zn( R )]ClO 4 with L = tris(pyridine-2-ylmethyl)amine (TPA), N , N -((5-estermethyl-2-pyridyl)methyl)-bis(2-pyridylmethyl) amine (5-COOCH 3 TPA), N , N -(bis(5-estermethyl-2-pyridyl)methyl)-(2-pyridylmethyl) amine [(5-COOCH 3 ) 2 TPA], or N , N -((6-estermethyl-2-pyridyl)methyl)-bis(2-pyridylmethyl) amine (6-COOCH 3 TPA) and R = 4-dimethylaminoflavonol [4-N(Me) 2 FLH], 4-diethylaminoflavonol [4-N(Et) 2 FLH], or 4-methoxyflavonol (4-MeOFLH), have been prepared and characterized. When dissolved in CH 3 CN or 1:1 H 2 O:dimethylsulfoxide (DMSO) in presence of O 2 , visible light ( λ max ranging from 417 to 462 nm) induced dioxygenase-type reactions to release CO quantitatively from these complexes. Moreover, the intracellular uptake and CO releasing reactivity were confirmed by the change of their fluorescent properties observed under fluorescence microscopy. The cytotoxicity of the complexes demonstrates that they exhibit good biocompatibility in the concentration range from 1 to 50 μM. The solid complexes are stable in air for more than 60 days, and their aqueous solutions are also stable for 96 h under anaerobic or dark condition. The remarkable stability and biocompatibility of the complexes are indispensable properties for use in pharmaceutical chemistry. Graphic abstract
研究领域:
无机化学
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药物化学
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生物化学
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DOI
高影响因子
Drug Delivery and Translational Research
·
2020
·Vol. 11
·期 1
·169-181
·
IF 2.664
Wu Jun-Yong,Cai Jia-Xin,Li Yong-Jiang,Hu Xiong-Bin,Liu Xin-Yi,Wang Jie-Min,Tang Tian-Tian,Xiang Da-Xiong
For therapy of skin cancer, transdermal administration has been a potential way to enhance chemotherapy. However, the drug delivery efficacy remained unsatisfactory because of the physiological barriers from the skin to the tumor, which hindered the effect of 3,5,4′-trimethoxy-trans-stilbene (BTM), a drug that has toxicity to cancer. Herein, we prepared an oil-in-water (O/W) microemulsion to load BTM (BTM-ME) for transdermal therapy of melanoma. BTM-ME was characterized by size, zeta potential, and polymer disperse index (PDI). B16F10 melanoma cell line was used for cell experiments and animal models. And cell uptake, viability assay, and flow cytometry were to test the cell internalization and the ability of BTM-ME to induce cancer cell apoptosis. Skin penetration testing was to detect its penetration efficiency to the skin. And tumor-bearing mice were used to prove the improvement of anti-cancer efficacy of BTM-ME with the combination of Taxol. BTM was successfully loaded in O/W microemulsion, with a drug loading capacity of 24.82 mg/mL. BTM-ME can penetrate the skin and increase the retention of BTM in the epidermis. And the combination of Taxol and BTM-ME effectively suppressed tumor growth and has lower toxicity to normal organs. BTM-ME provides adjuvant therapy to cutaneous melanoma and the combination of Taxol and BTM-ME has the clinical potential for skin cancer therapy.
Graphical abstract
研究领域:
药剂学
·
肿瘤学
·
药物递送系统
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透皮治疗
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DOI
PubMed
高影响因子
Microbial Cell Factories
·
2022
·Vol. 21
·期 1
·1-10
·
IF 6.352
Wen Yongdi,Liao Baosheng,Yan Xiaoxiao,Wu Zhenqiang,Tian Xiaofei
Background Hypocrellin A (HA) is a perylene quinone pigment with high medicinal value that is produced by Shiraia bambusicola Henn. (S. bambusicola) and Hypocrella bambusae (Berk. & Broome) Sacc. (Ascomycetes) with great potential in clinical photodynamic therapy. Submerged cultivation of S. bambusicola is a popular technique for HA production. However, there is not much research on how temperature changes lead to differential yields of HA production.Results The temperature regulation of submerged fermentation is an efficient approach to promote HA productivity. After a 32 °C fermentation, the HA content in the mycelia S. bambusicola (GDMCC 60438) was increased by more than three- and fivefold when compared to that at 28 °C and 26 °C, respectively. RNA sequencing (RNA-seq) analysis showed that the regulation of the expression of transcription factors and genes essential for HA biosynthesis could be induced by high temperature. Among the 496 differentially expressed genes (DEGs) explicitly expressed at 32 °C, the hub genes MH01c06g0046321 and MH01c11g0073001 in the coexpression network may affect HA biosynthesis and cytoarchitecture, respectively. Moreover, five genes, i.e., MH01c01g0006641, MH01c03g0017691, MH01c04g0029531, MH01c04g0030701 and MH01c22g0111101, potentially related to HA synthesis also exhibited significantly higher expression levels. Morphological observation showed that the autolysis inside the mycelial pellets tightly composted intertwined mycelia without apparent holes.Conclusion sThe obtained results provide an effective strategy in the submerged fermentation of S. bambusicola for improved HA production and reveal an alternative regulatory network responsive to the biosynthesis metabolism of HA in response to environmental signals.
研究领域:
微生物学
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生物技术
·
分子生物学
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DOI
PubMed
高影响因子
AMB Express
·
2022
·Vol. 12
·期 1
·1-15
·
IF 4.126
Liu Shasha,She Pengfei,Li Zehao,Li Yimin,Yang Yifan,Li Linhui,Zhou Linying,Wu Yong
According to a 2019 report from the Centers of Disease Control and Prevention (CDC), methicillin-resistant Staphylococcus aureus (MRSA) was listed as one of the “serious threats” that had become a global public challenge in hospitals and community. Biofilm-associated infections and refractory persisters of S. aureus also impede the effectiveness of conventional antibiotics that have greatly increased difficulty in clinical therapy. There is an urgent need to develop new antimicrobials with antibiofilm and anti-persister capacities, and drug repurposing is the most effective and most economical solution to the problem. The present study profiles the antimicrobial activity of ceritinib, a tyrosine kinase inhibitor, against S. aureus in vitro and in vivo. We investigated the antimicrobial efficacy of ceritinib against planktonic and persistent S. aureus by a time-killing kinetics assay. Then, antibiofilm effect of ceritinib was assessed by crystal violet staining and laser confocal microscope observation. Ceritinib showed biofilm inhibition and mature biofilm eradication, and possesses robust bactericidal activity against S. aureus persisters. We also evaluated antimicrobial efficacy in vivo using a subcutaneous abscess infection model. Ceritinib ameliorated infection in a subcutaneous abscess mouse model and only showed negligible systemic toxicity in vivo. Mechanism exploration was conducted by transmission electron microscopy, fluorescently labeled giant unilamellar vesicle assays, and a series of fluorescent dyes. In conclusion, we find ceritinib represents potential bactericidal activity against MRSA by disrupting cell membrane integrity and inducing reactive oxygen species production, suggesting ceritinib has the potential to treat MRSA-related infections.
研究领域:
微生物学
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药理学
·
传染病
·
药物再利用
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2022
·Vol. 189
·期 3
·1-11
·
IF 6.408
Wang Ying,Wang Tian,Wang Minglu,Wang Jun,Xu Zhixiang,Zhang Hongyan
The simple and rapid commercial colloidal gold test strip can only be used for qualitative or semi-quantitative detection, accompanied by weak detectability and false negative experimental results. Herein, a photothermal test strip assay which combined test strip with a portable photothermal card reader was established to achieve quantitative detection with excellent detectability. According to the photothermal effect produced by gold nanoparticles (GNPs) captured on the test line, the signal could be recorded by the reader. Thirteen food hazards including veterinary drug residues and pesticide residues were tested; the photothermal detectability in actual samples were about 23 (methyl parathion), 7 (enrofloxacin), 6 (sarafloxacin), 8 (sulfadiazine), 12 (sulfamethazine), 7 (paraquat), 6 (malachite green), 11 (amantadine), 13 (nitrofurazone), 6 (diethylstilbestrol), 12 (estriol), 21 (estrone), and 26 (17β-estradiol) times better than the visual detectability. Our results demonstrated that the photothermal test strip assay could be used for sensitive, rapid, and quantitative detection of residues of food hazards. Graphical abstract
研究领域:
食品科学
·
分析化学
·
纳米技术
·
传感器与执行器
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DOI
PubMed
高影响因子
3 Biotech
·
2020
·Vol. 10
·期 5
·1-13
·
IF 1.798
Liu Zhengying,Bao Jianying,Yang Huilin,Zhang Zhibin,Yan Riming,Zhu Du
Perylenequinones (PQ), a class of naturally occurring polypeptides, are widely used as a clinical drug for treating skin diseases and as a photodynamic therapy against cancers and viruses. In this study, the effects of different carbon sources on PQ biosynthesis by Shiraia sp. Slf14 were compared, and the underlying molecular mechanism of fructose as the sole carbon to enhance PQ production was investigated by transcriptome analysis. The results indicated that fructose enhanced PQ yield to 1753.64 mg/L, which was 1.73-fold higher than that obtained with glucose. Comparative transcriptome analysis demonstrated that most of the upregulated genes were related to transport systems, energy and central carbon metabolism in Shiraia sp. Slf14 cultured in fructose. The genes involved in glycolysis and pentose phosphate pathways, and encoding citrate synthase, ATP-citrate lyase, and acetyl-CoA carboxylase were substantially upregulated, resulting in increased overall acetyl-CoA and malonyl-CoA production. However, genes involved in gluconeogenesis, glyoxylate cycle pathway, and fatty acid synthesis were significantly downregulated, resulting in higher acetyl-CoA influx for PQ formation. In particular, the putative PQ biosynthetic cluster was upregulated in Shiraia sp. Slf14 cultured in fructose, leading to a significant increase in PQ production. The results of real-time qRT-PCR and related enzyme activities were also consistent with those of transcriptome analysis. These findings provide a remarkable insight into the underlying mechanism of PQ biosynthesis and pave the way for improvements in PQ production by Shiraia sp. Slf14.
研究领域:
分子生物学
·
微生物学
·
生物技术
·
药学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）