CANCER GENE THERAPY
·
2026
·
IF 6.4
Shi Ruxue,Gu Kai,Li Haichuan,Sun Qingwei,Wu Xinyu,Huang Shuhong,Hu Qingxia
Castration-resistant prostate cancer (CRPC) is one of the most prevalent cancers in men. The new generation androgen receptor (AR) inhibitor enzalutamide can improve the therapeutic effectiveness of patients with CRPC. However, these patients eventually develop acquired enzalutamide resistance (ENZR), and the mechanisms underlying resistance are not well understood. Wilms’ tumor 1-associating protein (WTAP) plays an important role in m 6 A modification and has been reported as an oncogene in various cancers. Here, we utilized a tissue microarray and collected tissues from prostate cancer (PCa) patients to detect WTAP expression, and found that WTAP is upregulated in PCa. Meanwhile, WTAP overexpression promotes cell proliferation and accelerates tumor growth through colony formation assays and the establishment of a subcutaneous xenograft model in vivo. These findings establish the tumor promoter role of WTAP in prostatic tumorigenesis. Furthermore, we verified that WTAP is a novel responsive gene of AR via promoter activity and chromatin immunoprecipitation (ChIP) assays. Importantly, we uncovered that WTAP is upregulated in ENZR cells, and WTAP knockdown inhibited the proliferation of ENZR cells. Mechanistically, ubiquitin-specific protease (USP7) enhanced the stability of WTAP by the ubiquitin-proteasome pathway in ENZR cells, thereby WTAP increases promote AKT signaling through an m 6 A-mediated way, and an AKT inhibitor can abolish the pro-resistance phenotype mediated by WTAP. Together, these findings suggest that WTAP plays a key role in ENZR development of PCa cells, and WTAP may be a potential treatment target for ENZR tumors. The alternative text for this image may have been generated using AI. In this manuscript, we utilized a tissue microarray and collected tissues from prostate cancer (PCa) patients to detect WTAP expression, and found that WTAP is upregulated in PCa. Meanwhile, WTAP overexpression promotes cell proliferation and accelerates tumor growth through colony formation assays and the establishment of a subcutaneous xenograft model in vivo. These findings establish the tumor promoter role of WTAP in prostatic tumorigenesis. Furthermore, we verified that WTAP is a novel responsive gene of AR via promoter activity and chromatin immunoprecipitation (ChIP) assays. Importantly, we uncovered that WTAP is upregulated in ENZR cells, and WTAP knockdown inhibited the proliferation of ENZR cells. Mechanistically, ubiquitin-specific protease (USP7) enhanced the stability of WTAP by the ubiquitin-proteasome pathway in ENZR cells, thereby WTAP increases promote AKT signaling through an m 6 A-mediated way, and an AKT inhibitor can abolish the pro-resistance phenotype mediated by WTAP. Together, these findings suggest that WTAP plays a key role in ENZR development of PCa cells, and WTAP may be a potential treatment target for ENZR tumors.
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PubMed
高影响因子
Science China-Chemistry
·
2026
·
IF 9.8
Chen Yi-Bo,Hao Jian,Liu Jia-Jia,Xiao Han,Lv Qi,Zhang Min-Yi,Li Shangda,Chi Lisheng,Zhong Yu-Wu,Ruhlmann Laurent,Zhang Jian,Li Chunsen,Fang Wei-Hui
Chiral metal-organic macrocycles and cages, as enzyme-mimetic platforms for asymmetric catalysis, face the challenge of integration with low-cost metals. We report the first homochiral Al lII macrocycle-based supramolecular cages, cAlOC-185- S/R —[(c-Al 8 ) 6 ], synthesized via a dual-ligand strategy combining chiral phosphates and N-donor co-ligands with HNO 3 . A distinctive trait is water coordination at the macrocycle’s waist, exposing catalytic sites. Additionally, switching N-donor coligands from NA to 6-NH 2 -NA modulates supramolecular assembly, enlarging the external channel diameter from 2.3 to 3.3 nm. Compared with traditional metal-organic cages, these macrocycle-based systems offer three key advantages. (1) Cost-effective: accessible ligands, low-cost Al III , one-pot synthesis. (2) Improved mass transfer: substrates react on the surface (no cage entry). (3) Tunable supramolecular channels. Benefiting from these structural and pore-regulating features, their benzaldehyde cyanosilylation efficiency is significantly enhanced: from 48 h, 91% yield, 82% ee to 12 h, nearly quantitative yield, 90% ee (rt), far outperforming free ligands (14% yield, no ee). Notably, most reported catalysts require −78 °C to 0 °C, with only ∼10% active at rt. Theoretical calculations confirm that enantioselectivity arises from the energy-favorable pathway. With 100-gram-scale synthesis validating its scalability, this work provides a low-cost, high-performance Al III catalyst and a versatile platform for catalyst development via precise pore engineering and theoretical guidance.
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Nature Synthesis
·
2026
·
IF 22.6
Jiang Tao,Qu Mengxin,Liu Xudong,Du Wei,Cha Shuangshuang,Meng Yuanyuan,Zhou Xinchi,Deng Zhongqiang,Gong Ming
Polysulfides are important reagents for sulfur-based polymers and lithium–sulfur batteries, yet their conventional syntheses yield varying chain-length mixtures. Electrochemical sulfide oxidation is an energy-efficient alternative route that suffers from electrode passivation, low selectivity and soluble products. Here we develop an in situ selective capturing strategy during sulfide electrooxidation to produce only trisulfide and tetrasulfide without disulfide in the precipitate form. By tuning electrolytic conditions and capturing agents, we achieve selectivities up to 73% (trisulfide) and 82% (tetrasulfide). This chemoselectivity stems from strong interaction of di-anionic polysulfides to hydrophobic quaternary ammonium cations that redirects the oxidation pathway to high-purity trisulfides and tetrasulfides. These trisulfide and tetrasulfide reagents crosslink halides or epoxides and vulcanize polyvinyl chloride at ambient conditions with sulfur chain-length-dependent properties, enabling precise polysulfide chemistry for advanced chemicals and materials. The alternative text for this image may have been generated using AI.
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Chinese Medicine
·
2026
·Vol. 21
·期 1
·116
·
IF 7.4
Yang Xiao,Liang Hangfei,Li Xuan,Tian Jianing,Fan Shicheng,Huang Min,Wan Jianbo,Zuo Zhong,Guo Haibiao,Bi Huichang
Background Mitochondrial biogenesis plays a vital role in various types of hepatocyte injury. Schisandrol B (SolB), a bioactive lignan isolated from Schisandra sphenanthera , exerts a significant hepatoprotective effect against lithocholic acid (LCA)-induced cholestatic liver injury. Whether mitochondrial biogenesis is involved in the anti-cholestasis effect of SolB remains unknown. Methods A mouse model of cholestatic liver injury was induced by intraperitoneal injection of LCA. SolB was administered orally twice a day. Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), total bile acids (TBA) and total bilirubin (TBILI), as well as hepatic superoxide dismutase (SOD) activity were measured. Liver pathology was evaluated by toxylin and eosin (H&E) staining. Mitochondrial morphology was examined using electron microscopy. Furthermore, the expression of mitochondrial biogenesis-related genes or proteins was analyzed by RT-qPCR or Western blot. Results We confirmed that SolB pretreatment (200 mg/kg/d) alleviated LCA-induced liver injury as evidenced by histological and biochemical analyses. SolB alleviated LCA-induced mitochondrial dysfunction in mice, as evidenced by increased mitochondrial DNA (mtDNA) content, superoxide dismutase (SOD) levels, and peroxisome proliferator-activated receptor γ co-activator 1α (PGC-1α) and mitochondrially encoded cytochrome c oxidase subunit 1 (MTCO1) expression, together with decreased fibroblast growth factor 21 ( Fgf21 ) and growth differentiation factor 15 ( Gdf15 ) gene levels. Transmission electron microscope analysis showed that LCA elicited small, fragmented mitochondria, which were not reversed after SolB pretreatment. However, western blot analysis showed that the expression of mitochondrial dynamics-related proteins, such as dynamin-related protein1 (DRP1), optic atrophy 1 (OPA1), mitofusin 1 (MFN1), and MFN2, was significantly decreased after LCA treatment. Pretreatment with SolB could significantly upregulate DRP1, mitochondrial fission factor (MFF), and fission1 (FIS1) which are crucial to regulate mitochondrial fission. It is worth noting that the protective effect of SolB against LCA-induced liver injury was independent of parkin RBR E3 ubiquitin-protein ligase (PARKIN)-mediated mitophagy as evidenced by decreased PARKIN and microtubule-associated protein light chain 3 (LC3)-II. Conclusion In summary, this study demonstrated that SolB improved mitochondrial function but had no effect on LCA-induced mitochondrial fragmentation, which provides new insights into better understanding hepatoprotective mechanism of SolB against cholestatic liver injury.
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高影响因子
MOLECULAR DIVERSITY
·
2026
·
IF 4.3
Hou Yingdong,Wang Zhijie,Xia Hubin,Zhou Yifeng,Zhang Xiaofeng
Dioctyl terephthalate (DOTP) has been associated with chronic intestinal inflammation. However, its implications for patients with colorectal cancer (CRC) remain unexplored. This study aimed to identify potential molecular targets and mechanisms involved in DOTP-related remodeling of the CRC microenvironment. Using multiple databases and weighted gene coexpression network analysis, we identified 12 potential DOTP-related CRC targets. Molecular docking (MD) and molecular dynamics simulations (MDS) were conducted to assess the interactions between these targets and DOTP. Notably, potential bindings were observed between DOTP and nine targets. Through the screening of 82 machine learning model combinations, four common targets were identified among the nine candidates, particularly RELA and AR. Importantly, exposure of SW620 and LS513 to DOTP resulted in increased RELA expression and enhanced proliferative and migratory capacities. Furthermore, elevated RELA expression was associated with an immunosuppressive microenvironment and a reduced response to immunotherapy. Single-cell RNA sequencing (scRNA-seq) revealed predominant RELA expression in macrophages, particularly in M2-like Mph_SPP1 cells, which exhibited marked immunosuppressive activity with highly expressed LGALS9. Notably, THP1 cells treated by DOTP resulted in increased mRNA expression levels of RELA and M2 phenotype markers. These findings suggest that dysregulation of the immune microenvironment may represent a critical mechanism underlying the progression of DOTP-related CRC and highlight RELA as a promising therapeutic target for CRC patients with high-risk exposure to DOTP.
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Journal of Coatings Technology and Research
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2026
·
IF 3.4
Wei Rong,Qi Yixing,Feng Guang,Zhang Juantao,Yuan Juntao,Lv Naixin,Fu Anqing,Zhang Qiuli
Microbiologically influenced corrosion (MIC), caused by microorganisms such as sulfate-reducing bacteria (SRB), iron bacteria (FB), and heterotrophic bacteria (TGB), accelerates metal oxidation and damages the protective film of coatings through their metabolic activities. Despite the development of various coatings, those capable of preventing microbiologically influenced corrosion (MIC) in metals remain limited. In this research, we have developed a novel type of coating that combines superhydrophobic and antibacterial characteristics, suppressing biofilm formation and preventing corrosion. Two antibacterial superamphiphobic coatings, termed as FPPDC and FPPTC, were created by synthesizing antibacterial fluorinated silica particles through a sol–gel process and incorporating them with two quaternary ammonium salts, i.e., DMOAP and TMAPS, respectively. The FPPDC coating outperforms the FPPTC counterpart in antibacterial performance, mechanical durability, and corrosion resistance. The FPPDC coating achieves a bactericidal efficiency of 99.99% against sulfate-reducing bacteria, higher than 99.00% for the FPPTC coating. Additionally, the FPPDC coating maintains its superhydrophobicity after 1200 abrasion cycles, while the FPPTC loses this property after 1000 cycles. The FPPDC coating successfully combines fluorinated chains and quaternary ammonium salts to achieve both antibacterial and anticorrosion functions, with excellent mechanical stability, making it suitable for MIC mitigation in oilfield environments. This research paves the way for new possibilities in developing multifunctional coatings.
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JOURNAL OF NANOBIOTECHNOLOGY
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2026
·Vol. 24
·期 1
·317
·
IF 15
Zhao Ting,Guo Lu,Cong Ning,Zhao Yading,Wang Xiaoxuan,Zeng Xinyu,Li Suyun,Liu Rui,Huang Shuting,Fu Yuye,Li Jie
ROS- and US-responsive targeted sGTND was constructed.
sGTND possesses excellent biocompatibility and exhibits ROS-responsive drug release.
sGTND responds to US to achieve UTMD effects and CEUI imaging.
sGTND/UTMD combination inhibits TCA cycle through NRF2 activation and G6PD inhibition.
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产品关联
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Cell Death & Disease
·
2026
·
IF 12.2
Ruan Banzhan,Wang Bingshu,Zhang Xiaodian,Liu Fujin,Wan Zhenling,Chen Yan,Wu Julan,Luo Chun,Lu Wenyan,Lu Yanda,Zheng Shaojiang
Pancreatic cancer is a highly lethal disease characterized by rapid onset, aggressive progression, and limited treatment options. The involvement of FoxM1 in the TGF-β/Smad signaling pathway has been linked to pancreatic cancer progression; however, the mechanisms behind the cooperative regulation of TGF-β signaling by FoxM1 and Smad4 remain poorly understood. In this study, we utilized molecular cytology techniques, animal models, and human pancreatic cancer tissues to investigate the role of FoxM1 in Smad4 stabilization and its regulation of TGF-β signaling. Our findings reveal that FoxM1 inhibits ubiquitin-proteasome-mediated degradation of Smad4, resulting in its stabilization. Once translocated into the nucleus, Smad4 binds to the FoxM1 promoter region, inducing FoxM1 expression and forming a positive feedback loop. Furthermore, we observed significantly higher expression of this feedback loop in pancreatic cancer tissues compared to adjacent normal tissues, with markedly elevated levels in poorly differentiated tissues compared to well-differentiated ones. Therefore, the loop aberrantly activates the TGF-β pathway, driving pancreatic cancer progression. These findings uncover a novel mechanism of TGF-β pathway activation and provide potential new targets for the prevention and treatment of pancreatic cancer. This study elucidates that FoxM1 functions to impede the ubiquitin proteasome-mediated degradation of Smad4, consequently stabilizing it. Following nuclear translocation, Smad4 binds to the FoxM1 promoter region, initiating FoxM1 expression and establishing a positive feedback loop. This loop plays a pivotal role in promoting pancreatic cancer development and migration by aberrantly activating the TGF-β pathway.
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Journal of Coatings Technology and Research
·
2026
·
IF 3.4
Chen Linyang,Xing Zheng,Liu Weiwei,Luo Jing
In this article, a series of acrylic ester copolymer hyperdispersants with different chemical structures (anchoring segments and solvated segments) were designed and synthesized by free radical polymerization and used to disperse mica in UV-curable systems. The influence of anchoring segments and solvated segments of the polyacrylic hyperdispersant on the dispersion of mica in UV-curable coating as well as the coating properties was systematically investigated. The dispersion performance of the hyperdispersants was investigated by rheology measurement, mechanical property, and anticorrosion property (via electrochemical impedance spectroscopy and neutral salt spray test) of the UV-curable coatings. It was found that the optimum anchoring segment and solvated segment of the polyacrylic hyperdispersant for dispersing mica in UV-curable systems were N-isopropylacrylamide (NIPAM) and butyl methacrylate (BMA), respectively. With the addition of p(BMA- co -NIPAM), the viscosity of the UV-curable resin system with mica decreased obviously. Moreover, the negative effect of mica on the mechanical properties of the UV-curable coatings was significantly mitigated, and the coating exhibited outstanding anticorrosion performance. The electrochemical low-frequency impedance value of the coating remains above 10 9.0 Ω cm 2 for 40 days without any decrease. After 1,200 h of neutral salt spray testing, only slight pitting corrosion was observed on the coating surface.
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JOURNAL OF MATERIALS SCIENCE
·
2026
·
IF 4.4
Kadir Aygul,Li Yan,Ahmant Ali,Yan Yin,Dong Xiaoyu
In this study, a high-performance self-powered ultraviolet photodetector (UV PD) is demonstrated, which is fabricated through straightforward hydrothermal and electrochemical deposition techniques. The device features a p–n heterojunction structure composed of ZnO nanorods (NRs) as the n -type bottom layer, poly(3,4-ethylenedioxyselenophene) (PEDOS) as the p -type top layer, and copper oxide (CuO) nanoparticles (NPs) incorporated as a key intermediate layer. Under weak UV irradiation (365 nm, 0.32 mW/cm 2 ), the fabricated UV PD delivers outstanding self-powered performance, attaining a responsivity of 0.185 μA/W and a detectivity of 3.58 × 10⁹ Jones. The CuO NPs insertion layer is verified as the key contributor to device performance enhancement: the UV PD with the CuO NPs interlayer exhibits a responsivity higher than that of the device without the interlayer. This performance boost is attributed to the CuO NPs’ ability to optimize heterojunction quality-specifically; it facilitates carrier generation, transport, and separation while extending carrier lifetime, thus substantially enhancing the responsivity of the CuO NPs/ZnO/PEDOS self-powered UV PD. Additionally, the fabricated self-powered UV PD exhibits excellent multi-cycle stability and robust detection performance under different light intensities. With its high efficiency and low-cost fabrication process, this self-powered UV PD exhibits great potential for practical applications in UV sensing systems.
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Nature Catalysis
·
2026
·
IF 48.3
Wu Huixin,Xiang Qian,Wang Liang,Wang Hai,Luo Qingsong,Lv Yating,Deng Lifan,Wu Yuexin,Hui Yu,Tang Rui,Yuan Wentao,Wang Yong,Wu Qing,Cao Xiao-Ming,Wang Liang,Xiao Feng-Shou
Hydrogenation of pyridines is particularly challenging because their strong coordination to metal sites hinders hydrogen adsorption and can poison the catalysts. Here we show that Ni nanoparticles (NPs) within zeolite crystals can overcome this problem, owing to a network of micropores that features a greater proportion of free Ni sites for hydrogen adsorption even in the presence of pyridines. As a result, a high activity was achieved under mild conditions, substantially outperforming those of the commercial Raney Ni and conventional supported Ni catalysts. In addition, Ni leaching from the Ni NPs within the zeolite crystals was effectively hindered under the reaction conditions, achieving excellent stability. The versatility of the approach was shown by synthesizing Pd NP analogues, which showed improved activity compared with the conventional supported Pd catalysts.
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CATALYSIS LETTERS
·
2026
·Vol. 156
·期 10
·275
·
IF 2.5
Hu Zhoulin,Li Jianwei,Zhang Chuanqi,Guo Wenzhi,Dai Bin,Zhang Chuancai
This study prepared and evaluated hollow spherical (B-Al 2 O 3 ) and needle-like (N-Al 2 O 3 ) alumina catalysts in a fixed-bed reactor for the transesterification of dimethyl oxalate (DMO) with ethanol (EtOH) to produce diethyl oxalate (DEO), using commercial alumina (C-Al 2 O 3 ) as a reference. Experimental results indicated that catalytic activity followed the order B-Al 2 O 3 > N-Al 2 O 3 > C-Al 2 O 3 . At 140 °C and a space velocity of 0.2 h –1 , B-Al 2 O 3 achieved a DMO conversion of 100%, with a DEO selectivity of 84.9% and an ethyl methyl oxalate (EMO) selectivity of 15.1%. The superior performance of B‑Al 2 O 3 originated from its largest specific surface area (202.52 m 2 /g), abundant weak acid‑base sites, and high oxygen vacancies that served as Lewis acid sites, providing more accessible active sites and promoting reactant activation. In situ DRIFTS results suggested a possible nucleophilic substitution pathway for the reaction, involving the replacement of CH 3 O − in DMO by C 2 H 5 O− from EtOH to form DEO. Deactivation studies revealed that coke deposition primarily caused catalyst deactivation; however, catalytic activity could be fully restored after regeneration. This study highlighted the morphological advantages of hollow spherical alumina and elucidated its structure–activity relationship, offering a design strategy for developing efficient catalysts for the green synthesis of DEO. Graphical
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