INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
·
IF 7.7
Guangjin Yu,Jing Wang,Qingshan Xiong,Yunqi Xu,Shouhu Xuan,Ken Cham-Fai Leung,Qunling Fang
With the prevalence of drug-resistant bacteria and the waning effects of antibiotics, nanoplatform has become an effective strategy for fighting infections. This work reports a dipolar-hollowed α-Fe 2 O 3 @Au/Polydopamine (PDA) nanospindle which possesses both photothermal-photodynamic (PTT-PDT) coupling antibacterial and drug carrying performance. Firstly, the spindle type α-Fe 2 O 3 @Au/PDA particle was prepared by a simple one-step strategy and then the dipolar-hollow structure was obtained by controlling etching the inside α-Fe 2 O 3 core with hydrochloric acid. After further loading the photosensitizer zinc phthalocyanine (ZnPc), the dipolar-hollowed α-Fe 2 O 3 @Au/PDA-ZnPc nanospindles were obtained. Owing to the dipolar-hollowed interior, the nanospindles are also effective in carrying antitumor drug doxorubicin (DOX) and shows a good drug loading-release behavior. The dipolar-hollowed α-Fe 2 O 3 @Au/PDA nanospindles exhibits a high antibacterial performance against Escherichia coli ( E. coli ) and Staphylococcus aureus ( S. aureus ) under near-infrared (NIR) and Xenon lamp irradiation. When α-Fe 2 O 3 @Au/PDA-ZnPc nanospindles concentration was increased to 100 μg/mL, the antibacterial rate was close to 100 %. In comparison to the original α-Fe 2 O 3 @Au/PDA nanospindles, the product achieved a lower effective antibacterial temperature. This triple-mode therapy (PTT/PDT/Drug) provides an interesting design idea for anisotropic therapeutic nanoplatform which can be applied in low-temperature antibacterial and drug delivery.
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Journal of Functional Foods
·
2024
·
IF 3.8
Zhizhou Li,Hui Wang,Shiwei Sun,Zhongbai Shao,Chaoyi Lv,Xiaoyue Dong,Lu Wang,Wei Wang
In the course of our research on utilizing edible flower resources to promote health, five ellagitannins ( 1 – 5 ) were isolated from pomegranate ( Punica granatum L.) flower by bioassay-guided fractionation. Among them, mallotusinin ( 1 ) and tercatain ( 2 ) were first isolated from pomegranate flower, and punicatannin D ( 4 ) was an unprecedented compound. Compounds 1 – 3 exhibited significant xanthine oxidase (XO) inhibitory activity compared with allopurinol, and a potent inhibition on α -glucosidase that was superior to acarbose was observed for compounds 1 – 4 . The XO inhibitory activity is positively correlated with the amount of galloyl moiety ( 3 > 2 > 1 ). Presenting tetrahydroxydibenzofuranoyl substituent or increasing the number of galloyl group enhanced the inhibition capacity of α -glucosidase ( 1 > 3 > 2 ). The inhibitory mechanism was further clarified in terms of kinetic studies and molecular docking. This study revealed that the ellagitannins present in pomegranate flower might be promising functional food ingredients with anti-hyperuricemia and anti-hyperglycemia properties.
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FOOD CHEMISTRY
·
2017
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IF 4.529
Hua Zhou,Hai-Mei Li,Yang-Min Du,Ri-An Yan,Shi-Yi Ou,Tian-Feng Chen,Yong Wang,Li-Xin Zhou,Liang Fu
YingDe black tea is produced from crude tea prepared from leaves of Camellia sinensis var. assamica . In this work, we isolated and identified five novel flavanones, namely, amelliaone A – E ( 1 – 5 ), along with seven known compounds 6 – 12 from the ethanol extract of YingDe black tea. The structures of these five novel phenolic compounds were determined using extensive 1D and 2D nuclear magnetic resonance spectroscopy experiments. The compounds were further evaluated for antioxidant, α -glucosidase inhibitory, and cytotoxic activities. Compound 1 exhibited higher α -glucosidase inhibitory activity with a half-maximum inhibitory concentration value (IC 50 ) of 10.2 µM compared with acarbose (18.2 µM).
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APPLIED SURFACE SCIENCE
·
2025
·
IF 6.9
Lu Chen,Zhengkai Wu,Xueying Cheng,Shuyu Chen,Jiaxin Zhao,Hang Li,Yingna Guo,Qingkun Shang
In this paper, Bi 4 O 5 I 2 /α-Bi 2 O 3 composite photocatalysts were successfully prepared by loading Bi 4 O 5 I 2 nanosheets on the surface of α-Bi 2 O 3 nanorods. The photocatalytic performance was investigated for the degradation of propranolol under visible light. Bi 4 O 5 I 2 /α-Bi 2 O 3 (30) could degrade 95 % of propranolol within 120 min, and the degradation rates of propranolol were 64.3 and 2.68 times that of α-Bi 2 O 3 and Bi 4 O 5 I 2 , respectively. The mineralization degree of propranolol reached 54.7 %, which was also much higher than that of Bi 4 O 5 I 2 and α-Bi 2 O 3 alone. Based on the results of high-resolution mass spectrometry (HRMS), the possible degradation pathways and products of propranolol were proposed, and the environmental toxicity of the degradation products was evaluated. The formation mechanism of Bi 4 O 5 I 2 /α-Bi 2 O 3 Z-type heterojunction was proposed by investigating the band structure and photoelectric properties of the composite photocatalyst. This work provides a successful example for the construction and application of new heterojunction photocatalysts.
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ANALYTICAL CHEMISTRY
·
2025
·
IF 6.7
Zhenzhen Cai,Zhiheng Huo,Gaosheng Li,Xu Cheng,Fang Xiao,Yuwan Du,Baiyi Zu,Xincun Dou
The specific fluorescent detection of α-methyltryptamine (AMT) presents a great challenge because similar amine groups and benzene rings exist in a variety of amines. Here, we show the precise modulation of the electron-withdrawing strength of the π-conjugate bridge in aldehyde-containing Schiff base-based fluorescent probes for ultratrace AMT discrimination. It is found that different electron-withdrawing groups −C6H4, −C6H2N2, and −C6H2Br2 as the π-conjugate bridge of the 2-dicyanomethylidene-3-cyano-4,5,5-trimethyl-2,5-dihydrofuran (TCF)-based probes can classify and identify organic amines with different amine nucleophilicities. Notably, the probe with −C6H2Br2 as the π-conjugate bridge, denoted as BrFS–TCF, which has the highest electrophilicity of the recognition site, shows a superior nM-level limit of detection (LOD) and an instant response time (<0.1 s) toward AMT. Especially, it shows an excellent selectivity facing the secondary amines, tertiary amines, aromatic amines, and even primary amines. The present strategy would provide a new pathway for chemical substances with similar structures and properties and especially for fighting against illegal drugs.
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FOOD CHEMISTRY
·
2025
·
IF 9.8
Mengxue Diao,Ziwei Li,Runhao Zhou,Xiaoxia Yan,Tiehua Zhang
Thymol showed good antimicrobial activity, however, the poor aqueous solubility limits it to apply in food industry. α-Lactalbumin can be used to delivery hydrophobic molecules, then enhancing their biological activities. The study investigates the potential of α-lactalbumin to expand the application range of thymol, further to evaluate the antimicrobial activity of the α-lactalbumin-thymol complexation. Multi-spectroscopy techniques and computational simulations have confirmed the successful complexation, driving mainly by van der Waals force. The α-lactalbumin-thymol complexation exhibited the superior antimicrobial activity than thymol against Escherichia coli and Staphylococcus aureus , as indicated by lower or comparable minimum inhibitory concentration (336 μg/mL and 224 μg/mL, respectively), fewer colony forming units, and larger inhibition zone diameters. Furthermore, α-lactalbumin enhanced the degree of membrane damage by thymol. The complexation preserved no obvious cytotoxicity against HeLa cells. This study indicates that the α-lactalbumin-thymol complexation chelates hold promise as natural antimicrobial agents in the food processing industry.
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MOLECULES
·
2025
·Vol. 30
·期 5
·980
·
IF 4.6
Bin-Chun Li,Bingbing Wu,Xueting Hou,Guo-Bin Ding
α-L-rhamnosidases play a key role in the metabolism and biodegradation of dietary flavonoid glycosides. We have developed a novel microplate spectrophotometric method to rapidly evaluate the conversion rates and substrate selectivities of mesophilic α-L-rhamnosidases towards citrus flavanone diglycosides by combining with a high-active and thermophilic β-D-glucosidase based on UV-visible spectral differences between citrus flavanone diglycosides and the corresponding aglycones under alkaline conditions. Furthermore, catalytic activities and enzyme kinetics of four α-L-rhamnosidases from human gut bacteria on various dietary flavonoid glycosides with different glycosidic bonds from various subclasses have been explored by HPLC. The α-L-rhamnosidase BtRha78A specifically removed the rhamnose group from the flavones, flavanones and flavonols diglycosides with the α-1,6 glycosidic bonds. Moreover, BtRha78A displayed higher catalytic activities on the rutinose group at 7-OH of the aglycones than at 3-OH. HFM-RhaA preferred to catalyze the flavones, flavanones and dihydrochalcones diglycosides with the α-1,2 glycosidic linkages at the 7-OH. However, this enzyme also showed high catalytic activity on the flavonol diglycoside rutin with the α-1,6 glycosidic bonds at the 3-OH. HFM-RhaC exhibited certain hydrolytic abilities towards all flavonoid diglycosides, and displayed higher activities on the flavonoid diglycosides with the α-1,6 glycosidic bonds. HFM-Rha78 weakly hydrolyzed the flavones, flavanones and dihydrochalcones diglycosides with the α-1,2 glycosidic bonds, and the flavonols diglycosides with α-1,6 glycosidic bonds. All four α-L-rhamnosidases from human gut bacteria did not exhibit catalytic activity towards the flavonoid glycosides with the α-1 glycosidic bonds. It was revealed that the α-L-rhamnosidases from human gut bacteria possessed diverse substrate selectivity on dietary flavonoid diglycosides. The structural basis for the specificity of BtRha78A on the flavonoid diglycosides with α-1,6 glycosidic bonds and the preference of HFM-RhaA on the flavonoid diglycosides with α-1,2 glycosidic bonds have been analyzed by molecular docking.
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ADVANCED FUNCTIONAL MATERIALS
·
2025
·
IF 19
Qiaoxuan Wang,Qi Cheng,Ding Huang,Kefei Zhao,Qiaoju Hu,Min Liang,Yifan Xie,Huaxiang Wu,Changyou Gao
The inefficient lysosomal escape of small interfering RNA (siRNA) from immune cells remains a major barrier to therapeutic efficacy. To address this challenge, an innovative pH-triggered aggregation nanoplatform for enhanced siRNA delivery is developed. By using adamantane (Ad)-modified hyperbranched poly-L-lysine (HBPL-Ad) as a backbone, this nanoplatform incorporated acetalated β-cyclodextrin (β-ACD) as a pH-sensitive “gatekeeper”, together with siRNA to prepare physiologically stable aggregated nanoparticles (ANPs). Triggered by the acidic lysosomal microenvironment, the β-ACD hydrolysis exposed β-CD cavities to enable strong host-guest (β-CD/Ad) binding. This binding caused nanoparticle aggregation and subsequent mild lysosomal membrane permeabilization (LMP), facilitating efficient siRNA escape independent of the proton sponge effect. The ANPs demonstrated superior cellular uptake and significantly enhanced TNF-α silencing across macrophages, dendritic cells, and Jurkat immune cells in vitro. Intraperitoneal administration of ANPs loaded with TNF-α siRNA in a collagen-induced arthritis (CIA) model achieved 93% mRNA reduction and 95% protein reduction in joint TNF-α levels, significantly outperforming Lipo2000 (56% mRNA and 60% protein reduction), effectively attenuating synovitis and cartilage erosion without detectable systemic toxicity. This work establishes pH-triggered programmable aggregation as a potent and transformative strategy for overcoming immune cell delivery barriers, offering a promising clinically viable platform for RNAi-based immunotherapy.
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Food Science & Nutrition
·
2025
·Vol. 13
·期 9
·e70862
·
IF 3.8
Lu Jin,Yuanquan Ran,Nian Yang,Lanlan Yang,Xiaoyan Jia,Huan Zhao,Qiong Hu,Bing Yang,Dongxin Tang,Minyi Tian
α-Caryophyllene is a natural condiment and flavoring additive. Herein, we first study the in vivo and in vitro anti-lung cancer efficacy of α-caryophyllene and its potential mechanism. In antitumor activity in vitro, α-caryophyllene exhibited obvious selective cytotoxicity, and its cytotoxicity against lung cancer A549 cells (IC 50 = 22.94 ± 0.56) was superior to the elemene injection (EI, positive control), while its toxicity against normal cell lines was lower (MRC-5: IC 50 = 76.66 ± 2.60 μg/mL, L929: IC 50 = 44.04 ± 0.72 μg/mL). α-Caryophyllene blocked the cell cycle in the G1 phase via reducing cyclin E2-CDK2 and cyclin D3-CDK 4/6 complexes and increasing p21, thereby suppressing A549 cell proliferation. Simultaneously, α-caryophyllene activated the mitochondrial apoptotic pathway through elevating the Bax/Bcl-2 ratio, lowering ΔΨm, releasing Cyt c, activating caspase 9 and caspase 3, and triggering PARP fragmentation. In addition, α-caryophyllene downregulated N-cadherin and MMP-2 levels, thereby preventing the metastasis of A549 cells. In antitumor activity in vivo, α-caryophyllene effectively suppressed transplanted tumor growth by inducing apoptosis of transplanted tumor cells and was superior to the positive control EI. Therefore, α-caryophyllene has outstanding anticancer properties in vitro and in vivo, showing great potential to become a new anticancer drug in the pharmaceutical field.
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TALANTA
·
2025
·
IF 6.1
Terefe Tafese Bezuneh,Fuchun Nan,Xin Li,Tongtong Kou,Zilong Wu,Fangfang Lang,William W. Yu
Designing highly sensitive method for the analysis of α-glucosidase activity is of critical value for diagnosis and therapy of diabetes. Here, we employed self-assembly of guanosine 5′-monophosphate nucleotide (GMP) with metal ions (Cu 2+ and Tb 3+ ) to prepare multifunctional Tb-CuGMP NPs. Owing to Cu 2+ -catalytic center, the as-prepared Tb-CuGMP NPs exhibits ascorbic acid oxidase (AAO)-like and peroxidase (POD)-like self-cascading catalytic activity. The AAO-POD cascade system can catalyze the oxidation of AA into dehydroascorbic acid (DHAA) with the generation of H 2 O 2 , which serves as a substrate for POD-like activity and catalyzed into • OH radicals. Based on these enzymatic reactions, a unique colorimetric detection approach in which nanozyme/TMB/AA produced oxidized TMB with characteristic blue color and DHAA sensitized Tb 3+ to turn-on the fluorescence of the detection system was proposed. Furthermore, enzymatic hydrolysis of 2-O-a-D-glucopyranosyl-L-ascorbic acid (AA2G) into AA was coupled with AAO-POD cascade system to realize highly sensitive detection of α-glucosidase activities. Interestingly, the as-constructed dual-mode assay reduces the LOD of α-glucosidase to 5.1 × 10 −4 U/mL. To sum up, this work provides new horizon for the development of efficient multifunctional nanomaterial with integration of biocatalysis and bioassay into a single material.
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Food Bioscience
·
2025
·
IF 5.9
Yifu Zhang,Jiawang Zhang,Kefan Guo,Menghang Zhou,Xixian Xie,Ye Chen
α-Cyclodextrin (α-CD) is characterized by its inherent hydrophobic cavity and favorable water solubility, serving as an effective means to enhance the solubility and stability of lipophilic substances. However, the encapsulation efficiency of α-CD inclusion complexes remains relatively low. Therefore,in this study, the regulatory mechanisms of self-assembled nanostructures of α-CD were explored. The structural differences of α-CD self-assembled via hydrogen bonds under various pH conditions were investigated. Taking Vitamin D 3 (VD 3 ) as an example, the impact of these structures on encapsulation efficiency was verified, and the α-CD-VD 3 inclusion complex (IC) was characterized. At pH 9, α-CD exhibited the smallest average particle size (134.36 ± 1.17 nm) and the highest zeta potential (−43.20 ± 1.94 mV), indicating excellent thermal stability. Fourier-transform infrared spectroscopy and proton nuclear magnetic resonance analyses revealed minimal hydrogen bonding between α-CD molecules at this pH, resulting in a maximum encapsulation efficiency of 89.27 % for α-CD-VD 3 . The continuous variation method confirmed the formation of the α-CD-VD 3 IC, with a binding constant of 265.32 M -1 and a Gibbs free energy of −3.30 kcal/mol. The α-CD-VD 3 aqueous solution demonstrated good stability under varying pH and salt ion conditions. In vitro release studies showed that the α-CD-VD 3 IC was stable in simulated gastric fluid and efficiently released VD 3 in simulated intestinal fluid. A stable aqueous delivery system based on the self-assembled structure of α-CD has been successfully developed in this study, which holds broad application prospects in various fields, including pharmaceutical and food sciences.
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POLYMER
·
2025
·
IF 4.5
Yinqiao Liu,Jialu Han,Wensheng Yan,Qiuran Jiang,Jiamei Luo,Liying Zhang,Jun Wang,Wanshuang Liu
Growing environmental concerns are driving the development of bio-based and recyclable thermosetting polymers. In this work, we synthesized a novel liquid cycloaliphatic epoxide containing silyl ether bonds from α-terpineol, a renewable monoterpene alcohol. After optimizing the accelerator content, the epoxy resin cured with methylhexahydrophthalic anhydride exhibited a high glass transition temperature (172 °C) and an acceptable tensile strength (57.2 MPa), the presence of silyl ether bonds imparts the resin with inherent degradability in fluoride and acidic organic solutions, offering a pathway for recycling. Finally, the optimized epoxy system was used to prepare a thermally conductive adhesive with boron nitride (BN) as the filler. The resulting epoxy composite 35 wt% BN, exhibited an enhanced thermal conductivity of 1.298 W/m·K. Crucially, the high-value BN fillers could be recovered non-destructively through selective chemical recycling of the epoxy matrix.
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