SEPARATION AND PURIFICATION TECHNOLOGY
·
2025
·
IF 9
Zewen Xu,Pei Li,Hao Guo,Shengchao Zhao,Kuo Chen,Xinliang Liu,Ming Wang,Yingfei Hou
Excessive carbon emission leads to global warming, threatening human survival. Membrane-based carbon capture technology is an important component of carbon capture, utilization, and storage (CCUS) technology and one of the main upstream technologies for achieving carbon neutrality. PIM-1 materials exhibit high CO 2 permeability but lower CO 2 /N 2 selectivity, making it a significant focus to improve CO 2 /N 2 selectivity without sacrificing CO 2 permeability. In this work, Noria was introduced as a block through the bottom-up approach into the PIM-1 molecular chain, forming Noria-based porous organic polymer (NPOP) microregions with excellent CO 2 affinity. Additionally, the presence of NPOP increases the interchain gaps, resulting in a higher free volume. Consequently, the separation membrane prepared under optimal conditions broke the 2008 upper bound, with a 43.6 % increase in CO 2 permeability and a 41.7 % increase in CO 2 /N 2 selectivity. Furthermore, NPOP could form colloidal networks and provide rigidity to the separation membrane, endowing it with excellent aging resistance. After 6 months of aging, the CO 2 permeability of 0.5 %Noria decreased by only 33.4 % (67.4 % for pure PIM-1), indicating its strong potential for widespread applications.
✓ 已验证
产品关联
DOI
高影响因子
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
·
2024
·
IF 4.2
Chunmeng Ding,Zhenguo Shen,Ruonan Xu,Yajing Liu,Mengyue Xu,Chenyu Fan,Dongyue Hu,Tian Xing
Aim To investigate the impact of exosomes released by Porphyromonas gingivalis -Lipopolysaccharide activated THP-1 macrophages and human periodontal ligament fibroblasts on hepatocyte fat metabolism. Results The liver of rats with experimental periodontitis showed obvious steatosis and inflammation compared with control rats. The culture supernatant of macrophages and human periodontal ligament fibroblasts (hPDLFs), when stimulated with Pg-LPS, induced lipogenesis in HepG2 cells. Furthermore, the lipid-promoting effect was effectively inhibited by the addition of the exosome inhibitor GW4869. Subsequently, we isolated exosomes from cells associated with periodontitis. Exosomes released by Pg-LPS-stimulated macrophages and hPDLFs are taken up by hepatocytes, causing mRNA expression related to fat synthesis, promoting triglyceride synthesis, and aggravating NAFLD progression. Finally, two sets of exosomes were injected into mice through the tail vein. In vivo experiments have also demonstrated that periodontitis-associated exosomes promote the development of hepatic injury and steatosis, upregulate SCD-1 expression and inhibit the AMPK signaling pathway. Conclusions In conclusion, we found that exosomes associated with periodontitis promote hepatocyte adipogenesis by increasing the expression of SCD-1 and suppressing the AMPK pathway, which indicates that close monitoring of the progression of stomatopathy associated extra-oral disorders is important and establishes a theoretical foundation for the prevention and management of fatty liver disease linked to periodontitis.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2024
·
IF 5.3
Bolun Mei,Yueru Wang,Jianhua Zhou,Xingchuan Yang,Yi Yu,Li Xu,Guoji Liu
To meet the demand of separating and purifying 1-naphthylamine from the reaction production and filling the solubility data gap, the solubility of 1-naphthylamine in twelve pure solvents (acetone, acetonitrile, ethyl acetate, n-butyl acetate, cyclohexane, n-heptane, methanol, ethanol, n-propanol, iso-propanol, n-butanol, and iso-butanol) was experimentally investigated in the range from 273.15 K to 308.15 K. It was found that the solubility of 1-naphthylamine increases markedly with increasing temperature in all solvent systems. The maximum solubility was 0.8978 (in the solvent of acetone, T = 308.15 K) and the minimum solubility was 0.005485 (in the solvent of n-heptane, T = 273.15 K). Then the experimental solubility data was correlated by the modified Apelblat model, Van't Hoff model, NRTL model, Wilson model, and Margules model. ARD% was calculated to assess the accuracy of the five equations and the results indicated that the modified Apelblat model fits optimally with an average ARD% value of 1.62 %. The effects of various physicochemical properties such as solvent polarity, hydrogen bond donor, hydrogen bond acceptor, and cohesive energy density on solute dissolution behavior were explored. In addition, the apparent thermodynamic properties of 1-naphthylamine in different solvents were analyzed based on modified Van’t Hoff model, which indicated the dissolution process of 1-naphthylamine in selected solvents is endothermic and entropy-driving. Finally, the effect of intermolecular interactions on the dissolution behavior was investigated at the molecular scale through molecular dynamics simulation studies, including molecular electrostatic potential surface, solvation free energy and radial distribution function analysis. This work will provide a fundamental guidance for the crystallization, separation, and purification of 1-naphthylamine.
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Wenming Shao,Junru Zhou,Yuzhen Huang,Ziyu Zhang,Yingwen Chen,Zhen Gao,Jiangfeng Ma,Bin Wu
The economic viability of cellulose biotransformation remains constrained by high enzyme costs, with processive endoglucanases emerging as promising candidates due to their dual-function hydrolysis mechanism. However, comprehensive kinetic and synergistic analyses of these enzymes are notably limited. This study investigates the kinetic properties of GH5 processive endoglucanase (M3-1) through various kinetic models. Inverse Michaelis-Menten analysis revealed M3-1's superior substrate recognition capacity, demonstrating 95.5 % productive binding site coverage compared to 48.8 % in non-processive endoglucanases. This enhanced efficiency is attributed to M3-1's distinctive structural features, particularly its open and deep cleft configuration. Pre-steady-state kinetics identified substrate association as the rate-limiting step, providing crucial direction for enzyme engineering efforts. Synergistic studies with cellobiohydrolase (CBH) demonstrated remarkable degradation synergy (DS value up to 8.2 on filter paper) and improved substrate resistance compared to traditional EG/CBH combinations. We propose a novel bidirectional degradation mechanism for the M3-1/CBH system, operating both inside-out and outside-in. The effectiveness of M3-1/CBH combination was further enhanced by up to 320 % through the addition of nonionic surfactants and expansin. These findings advance our understanding of processive endoglucanases and their potential applications in biomass conversion.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2025
·
IF 9
Haike Li,Zhiyun Kong,Huan Zhang,Xiaolei Wang,Xin Wei,Ruichao Yang,Xueyi Zhang
Organic solvent nanofiltration (OSN) membranes are suitable for separating drugs from solvents, especially heat-sensitive drugs. However, the trade-off effect between permeance and rejection limits the effective utilization of OSN membranes. In this study, a gel OSN membrane with high permeability-selectivity was prepared by bridging sodium alginate (SA) with HKUST-1 and then cross-linking with Ca 2+ ions. The interspersed distribution of HKUST-1 among the flexible polymer chains results in three-dimensional pores of the gel membrane, which enriches the solvent transport channels. Meanwhile, SA is cross-linked by Cu 2+ from HKUST-1 and Ca 2+ , which densifies the pore structure and enhances the solute rejection of the gel membrane. When the concentrations of HKUST-1, SA, and Ca 2+ are 2.5 wt%, the gel membrane (HKUST-1 2.5% -CaSA) has strong hydrophilicity (11.1°), a small pore size (0.59 nm), and a relatively high porosity (56.89 %). The ethanol permeance of the HKUST-1 2.5% -CaSA gel membrane reaches 43.8 L·m −2 ·h −1 ·bar −1 , with a tracycline (TC) rejection as high as 97 %. Moreover, the HKUST-1 2.5% -CaSA gel membrane is capable of achieving high rejection of TC in other organic solvents (acetone, ethyl acetate, and isopropanol). Even after being immersed in non-polar (n-hexane) and polar solvents (N,N-dimethylformamide, isopropanol, ethanol) for a period of 20 days, the separation performance of the gel OSN membrane remains consistent, confirming the excellent solvent resistance and potential in drug separation and solvent recovery.
✓ 已验证
产品关联
DOI
高影响因子
PROTEIN JOURNAL
·
2024
·
IF 1.9
Yuan Libo,Wang Ke,Fang Yuan,Xu Xiujuan,Chen Yingcun,Zhao Dongxin,Lu Kui
Cecropin A (1–7) is a cationic antimicrobial peptide which contain lots of basic amino acids. To understand the effect of basic amino acids on cecropin A (1–7), analogues CA2, CA3 and CA4 which have more arginine or lysine at the N-terminal or C-terminal were designed and synthesized. The interaction of cecropin A (1–7) and its analogs with DNA was studied using ultraviolet–visible spectroscopy, fluorescence spectroscopy and circular dichroism spectroscopy. Multispectral analysis showed that basic amino acids improved the interaction between the analogues and DNA. The interaction between CA4 and DNA is most pronounced. Fluorescence spectrum indicated that Ksv value of CA4 is 1.19 × 10 5 L mol −1 compared to original peptide cecropin A (1–7) of 3.73 × 10 4 L mol −1 . The results of antimicrobial experiments with cecropin A (1–7) and its analogues showed that basic amino acids enhanced the antimicrobial effect of the analogues. The antimicrobial activity of CA4 against E. coli was eightfold higher than that of cecropin A (1–7). The importance of basic amino acid in peptides is revealed and provides useful information for subsequent studies of antimicrobial peptides. Graphical Abstract
✓ 已验证
产品关联
DOI
PubMed
高影响因子
AICHE JOURNAL
·
2024
·
IF 3.5
Liming Xia,Bofeng Zhang,Gang Hou,Li Wang,Sibao Liu,Guozhu Liu
Structured catalysts coatings exhibit excellent performance on non-adiabatic gas–solid process, deriving from their enhanced heat and mass transfer properties. However, the coating catalysts prepared by traditional hydrothermal method of zeolite encapsulating noble metal are prone to spalling, especially under conditions of large flow rate and high temperature. Herein, we prepared PtZn clusters encapsulated in silicalite-1 zeolite coating on stainless steel with high bonding strength by an improved in situ growth method. The optimized catalyst exhibited ultra-thin, uniform, continuous, and high degree of crosslinking, thereby enhancing mass transfer and thermal stability. In propane dehydrogenation reaction, the optimized PtZn@S-1-R showed a high specific activity of 14.7 mol C 3 H 6 $$ {\mathrm{mol}}_{{\mathrm{C}}_3{\mathrm{H}}_6} $$ mol Pt −1 s −1 and a propylene selectivity above 99% at 600°C with a high weight hourly space velocity of 120 h −1 . The metal-encapsulated zeolite coating catalysts broaden the application avenue for heterogeneous catalysis with great application prospects.
✓ 已验证
产品关联
DOI
高影响因子
Theranostics
·
2025
·Vol. 15
·期 6
·2360
·
IF 13.3
Bin Li,Jingyi Liu,Wanyu He,Yanlin Zhou,Man Zhao,Cong Xia,Xiaoyue Pan,Zhihua Ji,Ruoyu Duan,Hui Lian,Kai Xu,Guoying Yu,Lan Wang
Rationale: Acute lung injury (ALI)/acute respiratory distress syndrome (ARDS) is a critical syndrome with a mortality rate of up to 40%, and it is characterized by a prominent inflammatory cascade. The inflammasome and pyroptosis play crucial regulatory roles in regulating various inflammatory-related diseases by serving as pivotal signaling platforms for inflammatory responses and mediating the release of substantial quantities of inflammatory factors. Our previous studies confirmed that GC-1, a clinical-stage thyroid hormone analog, effectively mitigated pulmonary fibrosis by restoring mitochondrial function in epithelial cells. However, the potential effects of GC-1 on macrophage inflammasome assembly and pyroptosis in lung injury as well as the underlying mechanisms, remain unclear. Methods: The effects of GC-1 on lung injury, oxidative damage and inflammation were evaluated in two murine models of ALI (LPS- or HCl-induced models) by assessing lung pathology, the concentrations of IL-1β and IL-18 in BAL fluid, inflammasome and the levels of inflammasome- and pyroptosis-related proteins. Additionally, the impact of GC-1 on ROS-mediated inflammasome assembly and pyroptosis was investigated by examining ROS levels, Nrf2 signaling, and inflammasome adaptor protein ASC levels in mouse alveolar macrophages and human THP-1 macrophages treated with LPS and ATP. The Nrf2 inhibitor ML385 and the mitochondrial-ROS inhibitor Mito-TEMPO were used to further elucidate the effect of GC-1 on the Nrf2-p53-ASC pathway. Results: GC-1 significantly alleviated inflammation and lung injury in ALI model mice, as indicated by pulmonary pathology, inflammatory cytokine levels, ROS production and pyroptosis rates. Consistently, GC-1 inhibited ASC recruitment and oligomerization in macrophages, which suppressed the gasdermin D-mediated release of IL-1β and IL-18. These findings indicated a reduction in inflammasome assembly and pyroptosis initiation. Further research revealed that GC-1 may mitigate oxidative stress induced by mitochondrial damage through Nrf2 signaling, thereby inhibiting the expression of ROS-activated p53 and the target gene ASC. This protective effect of GC-1 could be reversed by ML385 and mimicked by Mito-TEMPO. Conclusions: This study presents a novel mechanism for treating ALI in which GC-1 inhibits macrophage ROS-mediated inflammasome assembly and pyroptosis through Nrf2-p53-ASC pathway. These findings highlight the promising potential of the use of GC-1 as an anti-inflammatory and antioxidant drug in the treatment of ALI/ARDS.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Physical Chemistry C
·
2024
·
IF 3.3
Jiaojiao Yu,Yi Zuo,Gudan Li,Hong Yang,Xinwen Guo
To synthesize a high-performance titanium silicalite-1 (TS-1), this paper reports a simple and environmentally friendly approach to regulate the alkalinity in the synthesis of titanium silicalite-1 (TS-1) involving tetrapropylammonium hydroxide (TPAOH) and tetrapropylammonium bromide (TPABr). Essentially, the pH of the synthesis gel was controlled by n(TPAOH/TPA+), while keeping the total tetrapropylammonium cations (TPA+) constant. The study shows that at a relatively moderate alkalinity of pH = 10.7, TS-1 produced exhibits nanocrystalline microstructure favorable for diffusion, contains high contents of TiO4 and TiV, and delivers 94.3% of H2O2 conversion with a turnover frequency of 565.6 mol (mol h)−1 in propylene epoxidation. Through detailed analysis of crystallization processes of TS-1 at different alkalinities, this study unveils the effect of alkalinity on the rate of Si–Ti condensation, which dictates the formation of the framework structure and incorporation of Ti species into the framework. Essentially, only at moderate alkalinity of pH = 10.7, comparable Si–OH and Ti–OH condensation rates permit the highest amount of Ti being inserted in TS-1 as active (TiO4 + TiV) and limits the self-condensation of Ti species to form inactive anatase-TiO2.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF CHEMICAL THERMODYNAMICS
·
2012
·
IF 2.422
Wu Qian,Yingjie Xu,Hongye Zhu,Chaohong Yu
Densities and viscosities of the pure ionic liquid 1-methylimidazolium acetate ([Mim]Ac) and its binary mixtures with methanol, ethanol, 1-propanol, and 1-butanol were measured at temperature ranging from T = (293.15 to 313.15) K. The thermal expansion coefficient, molecular volume, standard entropy, and lattice energy of [Mim]Ac were deduced from the experimental density results. A simple linear equation was used to correlate the variation of viscosity of [Mim]Ac with temperature. Excess molar volumes V E and viscosity deviations Δ η for the binary mixtures at above mentioned temperature were calculated and fitted to the Redlich–Kister equation with satisfactory results. Excess molar volumes for {[Mim]Ac + 1-butanol} mixture have an S shape, while those for other mixtures have a negative deviation from ideal behaviour over the entire mole fraction range. Viscosity deviations are all negative deviation for {[Mim]Ac + alcohol} mixtures. The results were interpreted in terms of interactions and structural factors of binary mixtures.
✓ 已验证
产品关联
DOI
高影响因子
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
·
2025
·Vol. 27
·期 10
·5109-5116
·
IF 2.9
Zheng Zou,Zijie Xiao,Wenxin Dong,Wei Dang,Shusheng Pan,Xiaojun Su,Wei Zhang
As one of the commonly used methods for surface passivation of semiconductors, a heterostructure method was developed in this work to passivate surface traps of CsPbBr3 single crystals (SCs), and further, this method was correlated with carrier recombination processes. Herein, carrier recombination processes in bare and [MAPbCl3]0.34[CsPbBr3]0.66-covered CsPbBr3 SCs were studied using time-resolved spectroscopic techniques, including steady-state and time-resolved photoluminescence (TRPL) spectroscopy and time-resolved microwave photoconductivity (TRMC). By comparing the kinetics of TRPL and TRMC, we concluded that surface hole-trapping process dominates the TRPL kinetics of bare CsPbBr3 SCs and surface electron-trapping process dominates the slower decay component in TRMC. By studying carrier recombination processes of CsPbBr3 SCs with and without choline bromide (CB) additives, we found that the use of CB could introduce additional surface electron and hole traps. For CsPbBr3 SCs with a shell, we observed charge carrier transfer from the shell to the CsPbBr3 crystal. We found that the [MAPbCl3]0.34[CsPbBr3]0.66 shell can reduce the electron-trapping and hole-trapping rates by 2.2 times and 5.2 times, respectively, indicating that the [MAPbCl3]0.34[CsPbBr3]0.66 shell can passivate the surface traps of CsPbBr3 crystals effectively.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ELECTRONIC MATERIALS
·
2024
·
IF 2.2
Cao Yang,Zhang Yong,Wei Wei,Wang Chen,Zhao Xiaoyu,Chen Xudan,Lu Zhenhao
Organic–inorganic metal halides have recently garnered significant interest owing to their excellent photoluminescence performance and high stability. Therefore, a synthetic procedure was designed here to obtain (PPZ)ZnBr 4 (PPZ = 1-phenylpiperazine) nanocrystals (NCs) incorporating Pb 2+ and Sn 2+ ions, obtaining blue and orange light emitters, respectively. Photoluminescence quantum yields of blue and orange emissions were up to 43.2% and 29.9%, respectively, under 365-nm excitation. The prepared NCs also demonstrated long-term stability and humidity resistance. This study not only provided a deep comprehension of low-dimensional perovskites, but also established an avenue for the strategic design of high-performance light-emitting perovskites.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）