Journal of Functional Foods
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2017
·
IF 3.144
Jing-wan Li,Ke Yuan,Sui-cun Shang,Ying Guo
Type 2 diabetes (T2D) often leads to metabolic disorders. Hyperchloraemia, a common electrolyte metabolic disorder and caused by excessive intake of chloride-containing acidic agents. Thus, while berberine hydrochloride (BH) elicits a good hypoglycaemic effect, its nearly 10% chloride ion component is a potential hazard for T2D patients. In this study, we prepared four berberine organic acid salts (BOAs), including berberine citrate, berberine malate, berberine succinate, and berberine fumarate. T2D rats were administered BH or BOAs (500 mg/kg d) for 6 weeks. The body weights and levels of total cholesterol, triglycerides, fasting blood glucose, fasting insulin, and insulin resistance index in administered rats were normal. Levels of Na + , K + , Cl − , HCO 3 − , pH, pCO 2 and ‘ base excess (BE) in BH group showed significant differences compared with normal rats. These results suggest that BOAs are comparable to BH in terms of hypoglycaemic effects, but superior with regard to safety from hyperchloraemia in T2D rats.
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CATALYSIS LETTERS
·
2017
·Vol. 147
·期 7
·1802-1810
·
IF 2.799
Hong Runrun,He Yufei,Miao Chenglin,Feng Junting,Li Dianqing
Abstract Pd–Ir mesocrystals with rough surface and highly branched structure were synthesized through a facile co-reduction method. The morphology evolution shows the mesocrystals were formed by oriented attachment of nanoparticles, and abundant defects sites as well as synergetic effect between building units were formed during mesocrystals oriented attachment process. Therefore, the supported Pd–Ir mesocrystals show a significant enhancement in catalytic performance in hydrogenation of 2-ethylanthraquinone compared with conventional Pd–Ir nanoparticles. The high activity can be ascribed to high densities of defect sites in Pd–Ir MCs catalyst, which facilitated H 2 activation. In addition, the geometric effect of Pd–Ir MCs catalyst was responsible for inhibiting further hydrogenation of eAQH 2 to undesired consecutive byproducts. Graphical Abstract
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INTERNATIONAL JOURNAL OF THERMOPHYSICS
·
2024
·Vol. 45
·期 7
·1-14
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IF 2.5
Almasi Mohammad
A thorough evaluation of the thermophysical properties of ethylcyclohexane (ECH) mixed with 2-alkanols (2-propanol to 2-hexanol) is presented across the temperature range of 293.15 to 323.15 K. The focus of this study is on the density and viscosity behavior of these systems. The experimental results demonstrate positive deviations from ideality in excess molar volume, while viscosity deviations are negative for all examined mixtures. This observation suggests the presence of weak intermolecular interactions between ECH and the 2-alkanol. These findings are consistent with the self-association behavior of 2-alkanol and the nonpolar nature of ECH, which disrupts the associated structures of the alcohols. Furthermore, the Friction theory ( f-theory ) was employed to model the viscosity of the binary mixtures. The f-theory exhibits excellent agreement with the experimental data, with a maximum deviation of only 2.24% observed in the ECH + 2-butanol system. This minimal discrepancy underscores the f-theory's efficacy in accurately correlating the viscosity measurements.
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CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Yetong Li,Kailong He,Siyuan Zhao,Linyu Jiao,Huiyong Chen,Xiaoxun Ma,Chengyi Dai
Many researchers have extensively studied the alkylation of naphthalene with methanol for the preparation of high-value-added chemicals, especially methylnaphthalenes (MNs). Studies indicated that methanol generates a large number of methanol-to-olefin by-products on zeolites, resulting in low selectivity of the target product and poor stability of the catalyst, limiting its application in the industrial field. In this study, CO 2 hydrogenation was coupled with naphthalene alkylation, and the H 3 CO* active intermediate generated in situ was alkylated with naphthalene by using metal oxide-zeolite (OX-ZEO) bifunctional catalysts, which exhibited the ‘slow release’ phenomenon of the methylation reagent. It improved naphthalene conversion, suppressed deep alkylation and coke deposition side reactions and kept the catalyst stable for up to 500 h. A high-density and medium-strength Brønsted acid center facilitated the efficient migration of H 3 CO* active intermediates with the desorption of 2-methylnaphthalene (2-MN), which was particularly important for improving naphthalene conversion and 2-MN selectivity. The in situ IR showed that more Brønsted acid sites of Ga-S-1 effectively pulled the migration process of H 3 CO* species to the zeolitic acid center and increased the efficiency of C-C coupling compared to ZSM-5, which promoted the alkylation of H 3 CO* species with naphthalene. The results of chemisorption experiments showed that the medium-strength Brønsted acid site, which promoted the desorption of 2-MN, inhibited the deep alkylation reaction. The 2,6-dTBPy-IR results showed that the presence of fewer Brønsted centers on the outer surface of Ga-S-1 further optimized the product distribution and enhanced the selectivity of 2-MN in MNs to more than 90 %. Under optimal conditions, 80.1 % selectivity for MNs was obtained via ZnZrO x &Ga-S-1 bifunctional catalysts, with 97.4 % selectivity for 2-MN in MNs.
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Cells
·
2025
·Vol. 14
·期 6
·394
·
IF 5.2
Min Jiang,Jiaqi Hou,Qianqian Chai,Shihao Yin,Qian Liu
Pulmonary fibrosis due to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is the leading cause of death in patients with COVID-19. β-catenin, a key molecule in the Wnt/β-catenin signaling pathway, has been shown to be involved in the development of pulmonary fibrosis (e.g., idiopathic pulmonary fibrosis, silicosis). In this study, we developed a SARS-CoV-2-infected A549-hACE2 cell model to evaluate the efficacy of the A549-hACE2 monoclonal cell line against SARS-CoV-2 infection. The A549-hACE2 cells were then subjected to either knockdown or overexpression of the effector β-catenin, and the modified cells were subsequently infected with SARS-CoV-2. Additionally, we employed transcriptomics and raw letter analysis approaches to investigate other potential effects of β-catenin on SARS-CoV-2 infection. We successfully established a model of cellular fibrosis induced by SARS-CoV-2 infection in lung-derived cells. This model can be utilized to investigate the molecular biological mechanisms and cellular signaling pathways associated with virus-induced lung fibrosis. The results of our mechanistic studies indicate that β-catenin plays a significant role in lung fibrosis resulting from SARS-CoV-2 infection. Furthermore, the inhibition of β-catenin mitigated the accumulation of mesenchymal stroma in A549-hACE2 cells. Additionally, β-catenin knockdown was found to facilitate multi-pathway crosstalk following SARS-CoV-2 infection. The fact that β-catenin overexpression did not exacerbate cellular fibrosis may be attributed to the activation of PPP2R2B.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2025
·
IF 5.4
Xue Chen,Jiemei Bi,Haoyang Zhang,Mengtong Yuan,Jianjiao Wang,Narisu Hu
Periodontitis is a chronic inflammatory disease caused by dental plaque biofilm. To prevent disease progression and achieve successful periodontal tissue repair, it is necessary to simultaneously address the need for antibacterial, anti-inflammatory, and osteogenic effects within deep lesions. This study proposes a novel approach using a NIR light-responsive nanocomposite for enhanced ossification and combating bacterial biofilm infections in periodontitis. The nanocomposite incorporates polydopamine-coated calcium carbonate nanoparticles and BMP-2, which exhibits excellent photothermal conversion efficiency for bacterial eradication. Additionally, polydopamine also serves as an efficient ROS scavenger, thereby reducing the inflammatory response associated with periodontal disease. The core CaCO 3 interacts with BMP-2 to promote bone regeneration. This strategy demonstrates a synergistic effect by integrating PTT, ROS therapy, and osteogenic therapy in treating biofilm-induced periodontitis. Moreover, the CaCO 3 @BMP-2/PDA NPs reduced the protein expression levels of pro-inflammatory cytokines TNF-α and IL-1β and mitigated LPS-induced inflammation. This combination not only achieved critical protective effects but also fostered the promotion of osteoblast differentiation.
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Nutrients
·
2017
·Vol. 9
·期 12
·1301
·
IF 3.55
Yajing Fang,Weiwei Cao,Mengmeng Xia,Siyi Pan,Xiaoyun Xu
Flavonoids exhibit a broad range of biological activities. However, poor absorption of some flavonoids is a major limitation for use of flavonoids as nutraceuticals. To investigate the structure requirements for flavonoids intestinal absorption, transepithelial transport and cellular accumulation (CA) of 30 flavonoids were determined using the Caco-2 cell monolayer. The bilateral permeation of five types of flavonoids followed the order: flavanones ≥ isoflavones > flavones ≥ chalcones > flavonols. The concentration of flavonoids accumulated in cells did not correlate with cell penetration since the correlation coefficient between the apparent permeability coefficient (Papp) and their corresponding CA was poor (R2< 0.3). Most flavonoids exhibited a ratio of 0.8–1.5 forPapp A to B/Papp B to A, suggesting passive diffusion pathways. However, luteolin, morin and taxifolin may involve the efflux mechanisms. The quantitative structure-permeability relationship (QSPR) study demonstrated that the intestinal absorption of flavonoids can be related to atomic charges on carbon 3′ (QC3′), molecule surface area (SlogP_V3), balance between the center of mass and position of hydrophobic region (vsurf_ID1) and solvation energy of flavonoids (E_sol). These results provide useful information for initially screening of flavonoids with high intestinal absorption.Keywords:Caco-2;flavonoids;intestinal absorption;permeability;QSPR
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POLAR RESEARCH
·
2018
·
IF 1.5
Xiangning Han,Daicheng Liu
In this reported study, a novel high-performance thin-layer chromatography (HPTLC) method was developed for the detection and quantification of the toxic substance di(2-ethylhexyl) adipate (DEHA) in Antarctic krill. This procedure was based on the extraction of DEHA by ultrasonic solvent extraction with anhydrous ethanol, silica-gel column chromatographic separation, HPTLC detection and quantification using petroleum ether/ethyl acetate/ acetone/glacial acetic acid (29:1:0.5:2d*, v/v/v/v) as the developing solvent and bromine thymol blue solution as the chromogenic agent. The content of DEHA in freeze-dried Antarctic krill was found to be ca. 0.63 ± 0.05 mg/g. The structure of DEHA in the Antarctic krill was subsequently determined by gas chromatography–mass spectrometry (GC-MS) and infrared chromatography, which verified the presence of this compound in the krill. The HPTLC method exhibited excellent accuracy, with a recovery of 97.1–101.6% and good precision with a relative standard deviation of 2.47–4.90%. The DEHA in Antarctic krill oil was extracted by n-hexane and detected using the same method described above, which verified that DEHA was also present in krill oil at a concentration of ca. 2.16 ± 0.08 mg/g. The presence of DEHA in kill oil is very concerning because of its demonstrated harmful ecotoxicity, and since Antarctic krill is the key link in the food chain in the Antarctic coastal marine ecosystem. The adverse effects of DEHA on Antarctic krill and the source of DEHA will be explored in future research.
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JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY
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2024
·Vol. 333
·期 6
·2691-2696
·
IF 1.5
Wang Yi,Yang Jiajia,Sui Jixue,Wang Lan,Tang Jia,Yang Yu,Lu Yunyun,Dai Shuang,Cao Qi
Efficient separation of europium (Eu) from aqueous solution is pivotal for the sustainable development of nuclear industry and rare earth industry. In this work, bis(2-ethylhexyl) phosphate (P204) was employed to separate Eu(III) by solvent extraction method using kerosene as diluent. The extraction equilibrium could reach within 30-min contact time. The extraction ratio enhanced as the increased O:A phase ratio, extractant concentration, and temperature. However, the HCl introduction caused its clear descent. Combined with the slope method and FT-IR analysis, extraction mechanism could be unveiled, which is the coordination between P=O double bond and Eu(III) ions in a proportion of 3:1.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
·
IF 7.7
BingBing Wang,Jian-Zhong Xu,Shuai Liu,Zhi-Ming Rao,Wei-Guo Zhang
L-Tryptophan hydroxylation catalyzed by tryptophan hydroxylase (TPH) presents a promising method for synthesizing 5-hydroxytryptophan (5-HTP), yet the limited activity of wild-type human TPH2 restricts its application. A high-activity mutant, MT10 (H318E/H323E), was developed through semi-rational active site saturation testing (CAST) of wild-type TPH2, exhibiting a 2.85-fold increase in k cat / K m over the wild type, thus enhancing catalytic efficiency . Two biotransformation systems were developed, including an in vitro one-pot system and a Whole-Cell Catalysis System (WCCS). In the WCCS, MT10 achieved a conversion rate of only 31.5 % within 32 h. In the one-pot reaction, MT10 converted 50 mM L-tryptophan to 44.5 mM 5-HTP within 8 h, achieving an 89 % conversion rate, outperforming the M1 (NΔ143/CΔ26) variant. Molecular dynamics simulations indicated enhanced interactions of MT10 with the substrate, suggesting improved binding affinity and system stability. This study offers an effective approach for the efficient production of 5-HTP.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2024
·
IF 4.9
Xin Bai,Jian Nie,Siyao Cheng,Daohu Sheng,Jijun Xiao,Wei Dong
The development of non-viral gene delivery systems often requires balancing transfection efficiency and cytotoxicity, particularly when using cationic polymers. Poly(2-hydroxypropenimine) (PHP), with its hydroxyl groups, presents a more biocompatible alternative to traditional polymers such as polyethyleneimine (PEI). In this study, we explored the potential to enhance PHP’s transfection capabilities and reduce its cytotoxicity through a targeted fluorination strategy. Specifically, fluorinated alkyl groups were grafted onto PHP via an ethylene oxide ring-opening reaction, with the reaction conditions optimized for temperature, solvent, and time to ensure efficiency. Transfection experiments using HEK 293 cells demonstrated that fluorinated PHP variants, especially PHP-FH8 16 , significantly improved transfection efficiency, achieving a 75.1 % gene expression rate at an N/P ratio of 2, compared to 44.0 % for unmodified PHP. The transfection experiments in Hela cells also showed substantial transfection efficiency. The gene expression rate was 53.2 % at an N/P ratio of 2 compared to 31.6 % for unmodified PHP. Notably, the fluorinated polymers exhibited lower cytotoxicity and increased serum stability, which suggests their potential as safer gene delivery systems. This study carefully evaluates the feasibility of using fluorinated cationic polymers as next-generation gene delivery vectors, balancing efficacy and safety. It underscores the strategic application of fluorination technology in developing modified polymer vectors that could lead to more efficient and safer non-viral gene therapy solutions.
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SCIENCE OF THE TOTAL ENVIRONMENT
·
2025
·
IF 8
Yiming Sun,Ruili Zhang,Jiali Li,Yihan Hu,Haolin Zhang,Xiangjie Wang,Yi Yang,Haibin Wang,Ming Ge
2-Ethylhexyl diphenyl phosphate (EHDPHP) is a widely used organophosphorus flame retardant and plasticizer easily released into the environment. Its biological toxicity is of great concern. The lung is considered a possible target organ for EHDPHP, but currently, there are limited studies on the biotoxicity of EHDPHP in poultry lungs. Therefore, the lungs were selected as the target organ to study the toxic effects of EHDPHP on chicks and their mechanisms of action. In this study, 7-day-old chicks were gavaged with different concentrations of EHDPHP, and lung samples were collected at 14, 28, and 42 days after intragastric administration. Lung histopathological and ultrapathological changes were examined by paraffin section-HE staining and transmission electron microscopy. The levels of lung damage markers (LDH) and oxidative stress markers (GSH-Px, SOD, and MDA) were detected by applying the kit. In contrast, lung cell pyroptosis-related factors (NLRP3, ASC, NF-κB, Pro-Caspase-1, IL-18, and IL-1β) and inflammatory factors (IL-6 and TNF-α) were assessed by using the qRT-PCR, Western blot and ELISA techniques. The results showed that EHDPHP induced pathological morphological changes and elevated LDH content in chick lungs, decreased lung antioxidant enzymes (GSH-Px and SOD) activities, increased peroxidation product MDA content and up-regulated the expression levels of cellular pyroptosis factors (NLRP3, ASC, NF-κB, Pro-Caspase-1, IL-18, and IL-1β), and the synthesis and secretion of inflammatory factors (IL-6 and TNF-α) were promoted. The above changes were EHDPHP dose-dependent. The results indicated that EHDPHP induced oxidative stress in chick lungs, resulting in oxidative damage to the lungs, and, intriguingly, the cellular pyroptosis pathway was activated, which was also involved in the process of EHDPHP-induced inflammatory damage in chick lungs. The results of this study revealed for the first time the damaging effects and mechanisms of EHDPHP on chick lungs. Also, they provided a scientific basis for further exploring the mechanisms of toxicity damage, safe use, and pollution control of EHDPHP.
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