NANO
·
2018
·
IF 1.1
Caiwei Li,Weimeng Si,Yuting Wu,Cheng Zhang,Wu Lei,Qingli Hao
Poly(3-methylthiophene)/reduced graphene oxide (P3MT/RGO) modified glassy carbon electrode (GCE) was fabricated via a facile procedure. P3MT was directly electrodeposited on the RGO coated electrode and employed for the electrochemical detection of paracetamol (PCT). The morphology of composite was characterized by scanning electron microscopy (SEM). Cyclic voltammetry (CV) and differential pulse voltammetry (DPV) were used to investigate the electrochemical behaviors of PCT on P3MT/RGO/GCE. Experimental parameters, such as the scan rates, polymerization laps and the pH of buffer solution were optimized. Under the optimal conditions, the anodic peak current in DPVs varies linearly with the concentration of PCT, and the limit of detection was 0.025 μ M. Moreover, the proposed P3MT/RGO/GCE also exhibited superior stability and reproducibility.
✓ 已验证
DOI
高影响因子
APPLIED SURFACE SCIENCE
·
2019
·
IF 5.155
Hong-bo Huang,Shao-hua Luo,Cai-ling Liu,Yue Yang,Yu-chun Zhai,Long-jiao Chang,Ming-qi Li
Na super ionic conductor (NASICON)-type Na 3 V 2 (PO 4 ) 3 (NVP) has been considered as a potential positive electrode material for sodium ion batteries (NIBs) owing to its high theoretical specific capacity. Nonetheless, the practical application of NVP is hindered by the intrinsically poor electronic conductivity. Herein, polydopamine-derived nitrogen-doped carbon-covered Na 3 V 2 (PO 4 ) 3 /C composites (NVP/C/NC) have been prepared through a self-polymerization of dopamine on the NVP/C surface and subsequent calcination at high temperature. The as-synthesized NVP/C/NC composite cathode exhibits a high initial reversible capacity (109.2 mAh/g at 0.2C), superior rate performance (87.2 mAh/g at a rate up to 20C), and excellent cycling capability (91.2% of the initial capacity is kept after 500 cycles at 2C) in NIBs. Furthermore, compared with NVP/C electrode, the NVP/C/NC electrode presents low resistance and high sodium ions diffusion coefficient . The good performance can be ascribed to the nitrogen-doped carbon layer in improving the electronic conductivity, shortening diffusion length of Na + ions and electrons, and relieving the volume changes of electrode materials. These preliminary results suggest that the as-obtained NVP/C/NC composite is a novel promising electrode material for low-cost sodium energy storage.
✓ 已验证
产品关联
DOI
高影响因子
CURRENT MICROBIOLOGY
·
2019
·Vol. 76
·期 6
·706-712
·
IF 1.595
Li Minghua,Meng Xiumei,Sun Zhiyang,Zhu Chunjie,Ji Huiying
It is well known that cofactors play a key role in the production of different compounds in bioconversion processes, while the high cost of cofactors limits their usage in industrial applications. In the present study, a NADH regeneration system was successfully developed in Lactobacillus plantarum by expressing the fdh gene coding for formate dehydrogenase (FDH) from Candida boidinii. Results indicated that the FDH was expressed with the highest activity of 0.82 U/mg of protein when cells entered early stationary phase. In addition, the expression of FDH increased the intracellular level of NADH and NADH/NAD + ratio in L. plantarum , and therefore, enhanced the NADH-dependent production of 3-phenyllactic acid (PLA) in repeated and fed-batch bioconversions. In brief, the results demonstrate that the NADH regeneration by expressing FDH is a promising strategy for producing NADH-dependent microbial metabolites in L. plantarum .
✓ 已验证
产品关联
DOI
PubMed
高影响因子
GREEN CHEMISTRY
·
2019
·Vol. 21
·期 16
·4434-4442
·
IF 9.405
Teng Li,Shengqin Liu,Bing Wang,Jingen Long,Jun Jiang,Ping Jin,Yao Fu,Haizhu Yu,Weiran Yang
Glycerol, generated in abundance as the by-product in the process of biodiesel production and saponification, has seen attempts to convert it into value-added chemicals. However, due to the low selectivity of hydrogenolysis of the secondary hydroxyl group, valuable 1,3-substituted chemicals are difficult to obtain from glycerol by chemocatalysis. In this work, glyceric acid (GA), a renewable biomass from glycerol, was quantitatively converted to 3-iodopropionic acid (3-IPA) at 373 K in 3 h by hydroiodic acid mediated hydrogenation. As the reductant in this process, HI is oxidized to I2 and then regenerated in situ by metal catalysts and H2. The reaction pathway was proposed by intermediate identification and verified by a kinetics study and computational method. The catalytic system was shown to be stable and can be reused several times without loss in activity. As a 1,3-substituted chemical, 3-IPA is not only a potential monomer to form poly-3-hydroxypropionic acid, but also a good platform molecule to produce useful chemicals, e.g. 3-hydroxypropionic acid (3-HPA) and acrylic acid (AA), due to its highly reactive nature.
✓ 已验证
产品关联
DOI
高影响因子
RSC Advances
·
2019
·Vol. 9
·期 43
·24904-24914
·
IF 3.049
Zengfeng Wei,Xin Chen,Jiang Duan,Caihong Mei,Dan Xiao,Aidong Zhang
Urushiol is a resource-limited natural coating material with diverse applications; however, the synthesis of urushiol analogues and the realization of their urushiol-like performance remain challenging. Herein, four urushiol analogues, namely, 3-((4-alkenoylpiperazin-1-yl)methyl)catechols with the precise 3-substitution pattern on a catechol as that found in urushiol were synthesized by employing the Mannich reaction of catechol with formaldehyde and N-Boc-piperazine as the key step in a two-step route. By using optimization, the advantages of convenience in operation, cost-effectiveness, and scalability could be obtained. The electropolymerization of these analogues on copper was found to be practical due to their higher aerobic stability than urushiol, affording robust coatings with desirable hardness, adhesion strength, hydrophobicity, and thermal stability. Furthermore, the coatings exhibited effective corrosion protection on copper with initial anticorrosion efficiency up to 99.9% and comparatively higher efficiency (more than 97%) after 4 weeks of immersion in 3.5 wt% NaCl solution. The evidence from the electrochemical and infrared spectroscopic characterization data revealed that the electropolymerization process mechanically involved the free radical coupling of phenoxyl radicals to themselves and to the CC bonds in the side chain, forming a robust crosslinking coating. This work paves a way for the synthesis of high-performance urushiol analogues with potential applications as metal protection materials.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2024
·Vol. 16
·期 16
·2472-2477
·
IF 2.7
Chenhao Zhao,Xingdan Wang,Jing Wu,Yeli Hu,Qiwei Zhang,Qi Zheng
Sialic acids are a family of monosaccharides that share a nine-carbon backbone and a carboxyl group. A recent derivatization method based on 3-nitrophenylhydrazine (3-NPH) provides a mild chemical labeling technique for biomolecules containing carbonyl or carboxyl groups. In this study, we utilized 3-NPH to label sialic acids via a two-step derivatization process. The derivatized species can produce a common reporter ion corresponding to C1–C3 with two labels, and a fragment differentiating between Neu5Ac, Neu5Gc, and KDN. This method is compatible with O-acetylated sialic acids and provides high sensitivity to Neu5Gc and KDN, and since the utilization of dual labeling significantly enhances the hydrophobicity of derivatives, it can effectively mitigate matrix effects when combined with parallel reaction monitoring technology. Negative-ion tandem mass spectrometry (MS/MS) analysis reveals a distinctive fragmentation profile for the 4-O-acetylated species, while the other sialic acids yield similar MS/MS spectra with a high abundance of reporter ions. Using the reporter ion as a transition, this analytical strategy is effective for analyzing complex biological samples. For example, it was successfully employed to quantify sialic acids in the intestinal tissues of several carp species, demonstrating its potential in sialylation research.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MOLECULAR CELL
·
2024
·
IF 14.5
Wei Luo,Yunyuan Xu,Jie Cao,Xiaoyu Guo,Jingdan Han,Yuanyuan Zhang,Yuda Niu,Meiling Zhang,Yi Wang,Guohua Liang,Qian Qian,Song Ge,Kang Chong
While it is known that temperature sensors trigger calcium (Ca 2+ ) signaling to confer cold tolerance in cells, less is known about sensors that couple with other secondary messengers. Here, we identify a cold sensor complex of CHILLING-TOLERANCE DIVERGENCE 6 (COLD6) and osmotin-like 1 (OSM1), which triggers 2′,3′-cyclic adenosine monophosphate (2′,3′-cAMP) production to enhance cold tolerance in rice. COLD6, which is encoded by a major quantitative trait locus (QTL) gene, interacts with the rice G protein α subunit (RGA1) at the plasma membrane under normal conditions. Upon exposure to chilling, cold-induced OSM1 binds to COLD6, kicking out RGA1 from interaction. This triggers an elevation of 2′,3′-cAMP levels for enhancing chilling tolerance. Genetic data show that COLD6 negatively regulates cold tolerance and functionally depends on OSM1 in chilling stress. COLD6 alleles were selected during rice domestication. Knockout and natural variation of COLD6 in hybrid rice enhanced chilling tolerance, hinting design potential for breeding. This highlighted a module triggering 2′,3′-cAMP to improve chilling tolerance in crops.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
·
2025
·
IF 3.3
Guo Jing,Cui Jiayi,Xun Mingyue,Zhang Wencheng,Xian Mo,Zhang Rubing
L- threo -3-hydroxyaspartic acid (L-THA) is a non-proteinogenic amino acid that has garnered significant attention due to its diverse biological activities. However, the synthesis of L-THA through enzymatic and whole-cell catalysis requires the expensive substrate L-aspartic acid or L-asparagine, and co-substrate α-ketoglutarate, which limits their large-scale application. Here, this is the first report of engineering E. coli as a cell factory for de novo production of L-THA from glucose by fermentation. Firstly, the asnO gene encoding asparagine hydroxylases from Streptomyces coelicolor was heterologously expressed in E. coli to yield the L-THA producing strain. The formation and configuration of L-THA were characterized by LC–MS and HPLC after FDAA derivatization. Secondly, the pathway genes aspC and asnB , which encode aspartate aminotransferase and asparagine synthase, respectively, were overexpressed to enhance L-THA titer from 49.9 to 90.84 mg/L. Thirdly, the efforts were made to improve the key precursor L-aspartic acid pool by overexpressing the aspartase encoding gene aspA and knocking out aspartate kinase (AK) III encoding gene lysC . The best strain CC03 was obtained and L-THA titer reached 278.3 mg/L in a shake flask, representing an approximately 5.6-fold increase compared to the original strain. Ultimately, 2.87 g/L L-THA was obtained after 32 h fed-batch fermentation. This research underscores the potential use of E. coli fermentation as a feasible platform for de novo biosynthesis of L-THA from glucose, which is amenable to industrial application.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF WUHAN UNIVERSITY OF TECHNOLOGY-MATERIALS SCIENCE EDITION
·
2017
·Vol. 32
·期 4
·772-776
·
IF 0.447
Xiang Tao,Wang Qingxiu,Duan Lijie,Liu Fude
A conjugated polymer of poly(4,4'-bis(thien-2-yl)azobenzene-co-3-hexylthiophene) (PTAB-H), bearing azo group in main chain and soluble in THF, chloroform, was synthesized through FeCl3 oxidation copolymerization with 3-hexylthiophene, and the monomer of 4,4'-bis(thien-2-yl)azobenzene was synthesized by Sandmeyer reaction and Suzuki reaction. The UV-Vis spectra of PTAB-H reveal that azo group in the main chain can extend the absorption and lower the band gap. The fluorescent spectra, photoluminescence (PL) spectra, cyclic voltammetry (CV) of PTAB-H were recorded.The gel permeation chromatography (GPC), thermogravimetry analysis (TGA), and differential scanning calorimetry (DSC) measurements indicated that the molecular weight Mn, Mw and polydispersity indices (PDI) of the polymer are 10 876 g/mol, 22 338 g/mol and 2.05 respectively and the temperature of 5% weight-loss is 224.6 °C. No glass transition and crystallization melting peak was observed in the temperature range from 30 °C to 400 °C.
✓ 已验证
产品关联
DOI
高影响因子
BIOPROCESS AND BIOSYSTEMS ENGINEERING
·
2017
·Vol. 40
·期 6
·901-910
·
IF 1.87
Zhang Qiang,Gong Jin-Song,Dong Ting-Ting,Liu Ting-Ting,Li Heng,Dou Wen-Fang,Lu Zhen-Ming,Shi Jin-Song,Xu Zheng-Hong
3-Hydroxypropionic acid (3-HP) is an important platform chemical in organic synthesis. Traditionally, 3-HP was produced by chemical methods and fermentation process. In this work, a novel enzymatic method was developed for green synthesis of 3-HP. A yeast strain harboring nitrile-hydrolyzing enzyme was newly isolated from environmental samples using 3-hydroxypropionitrile (3-HPN) as the sole nitrogen source. It was identified to be Meyerozyma guilliermondii CGMCC12935 by sequencing of the 18S ribosomal DNA and internal transcribed spacer, together with analysis of the morphology characteristics. The catalytic properties of M. guilliermondii CGMCC12935 resting cells were determined, and the optimum activity was achieved at 55 °C and pH 7.5. The enzyme showed broad substrate specificity towards nitriles, especially 3-HPN, aminoacetonitrile and 3-cyanopyridine. The presence of Ag + , Pb 2+ and excess substrate inhibited the enzyme activity, whereas 5% (v/v) ethyl acetate had a positive effect on the enzyme activity. M. guilliermondii CGMCC12935 resting cells by addition of 3% glucose could thoroughly hydrolyze 500 mM 3-HPN into 3-HP within 100 h and the maximal accumulative production of 3-HP reached 216.33 mM, which was over twofolds than the control group with no additional glucose. And this work would lay the foundation for biological production of 3-HP in industry.
✓ 已验证
DOI
PubMed
高影响因子
ChemSusChem
·
2016
·Vol. 9
·期 10
·1118-1124
·
IF 7.116
Xuefeng Liu,Zipeng Xing,Hang Zhang,Wenmei Wang,Yan Zhang,Zhenzi Li,Xiaoyan Wu,Xiujuan Yu,Wei Zhou
Graphical Pass the Mo : A 3 D sandwich of mesoporous black TiO 2 (MBT)/MoS 2 /MBT was fabricated using a facile mechanochemistry method combined with an in situ solid-state chemical reduction approach. The material exhibited excellent visible-light-driven photocatalytic performance for pollutant degradation and hydrogen evolution owing to the sandwich-like heterojunction structure and Ti 3+ doping. A novel 3 D mesoporous black TiO 2 (MBT)/MoS 2 /MBT sandwich-like nanosheet was successfully fabricated using a facile mechanochemical process combined with an in situ solid-state chemical reduction approach, followed by mild calcination (350 °C) under an argon atmosphere. The MBT/MoS 2 /MBT exhibits a 3 D sandwich-like nanosheet structure and heterojunctions are formed at the interfaces between MoS 2 and black TiO 2 . The significantly narrowed band gap of MBT/MoS 2 /MBT is attributed to the introduction of MoS 2 and the formed Ti 3+ species in the frameworks. The visible-light photocatalytic degradation rate of methyl orange and the hydrogen production rate are as high as 89.86 % and 0.56 mmol h −1 g −1 , respectively. The introduction of MoS 2 and Ti 3+ in the frameworks favors the visible-light absorption and the separation of photogenerated charges, and the 3 D sandwich-like heterojunction structure facilitates the transfer of photogenerated charges.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2016
·Vol. 163
·期 13
·D707
·
IF 3.014
Jie Zhang,Maozhong An,Anmin Liu,Shanshan Ji,Ye Lian,Xuefeng Ren
Octahedral indium particles are electrodeposited on the molybdenum films from 1-butyl-3-methylimidazolium trifluoromethanesulfonate ([Bmim][TfO]) ionic liquid. Chronoamperometry and SEM images demonstrate that the nucleation of indium on the glassy carbon electrode involves an instantaneous process. Molecular dynamic simulation reveals the interaction between [Bmim]+ and [TfO]− ion pairs and the indium surfaces, which can explain the formation of polyhedral shapes of the particles.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）