CELL BIOLOGY AND TOXICOLOGY
·
2025
·Vol. 41
·期 1
·1-17
·
IF 5.9
Mao Shitao,Yu Na,Wang Wei,Mao Yikai,Du Ying,Zhao Qihe,Gu Xiu,Kang Jian
Idiopathic pulmonary fibrosis (IPF), characterized by fibroblast activation and collagen deposition, is a progressive lung disease that lacks effective interventions. Ubiquitin-specific peptidase 10 (USP10) acts as a multifunctional player in inflammatory response and progression of cancers, the effect on pulmonary fibrosis is unknown. Here, we demonstrated downregulated expression of USP10 in fibrotic lung tissues of IPF patients. In the current study, lung tissues were collected at the end of weeks 1, 2, or 3 post bleomycin (BLM)-intratracheal delivery. Consistently, USP10 expression levels were reduced after BLM challenge in a time-dependent manner. Mice treated with lentivirus overexpressing USP10 exhibited mitigative lung injury and reduced collagen deposition. USP10 overexpression enhanced autophagy in BLM-treated mouse lungs. Interestingly, the protective effect of USP10 was attenuated as the pulmonary autophagy flux was blocked by autophagy inhibitor 3-methyladenine (3-MA). Primary human and mouse lung fibroblasts were treated with pro-fibrotic TGF-β1 to verify the role of USP10 in vitro. Mechanically, the deubiquitinating enzyme USP10 interacted with Sirtuin 6 (Sirt6) and inhibited its degradation. Furthermore, USP10 overexpression inhibited the activation of Sirt6-mediated AKT/mTOR pathway in both lung tissues and fibroblasts. Our findings suggest that USP10 might attenuate pulmonary fibrosis through the promotion of Sirt6/AKT/mTOR-mediated autophagy. These data prioritize USP10 as a therapeutic target for treating IPF. Graphical
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2025
·
IF 4
Xiangxuan Feng,Yaohui Wang,Yifan Wang,Jun Ren
The dysregulated overproduction of pro-inflammatory cytokines like interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) is a hallmark of severe inflammatory diseases. Extracorporeal blood purification offers a promising therapeutic strategy, but current adsorbents often lack the specificity required for precise intervention. This study reports the development of a bispecific nanobody (bsNb)-based immunoadsorbent for the simultaneous and specific removal of IL-6 and TNF-α. The functional bsNb, produced in a prokaryotic system via tandem fusion, was covalently immobilized on Sepharose CL-6B matrix to prepare the affinity adsorbent. Comprehensive characterization confirmed its high saturation adsorption capacity (6.9 mg/mL for IL-6 and 2.2 mg/mL for TNF-α) and rapid binding kinetics. In a simulated hemoperfusion system using human serum, the adsorbent achieved removal efficiencies exceeding 85% for both cytokines across a range of pathophysiological concentrations. Crucially, the bsNb adsorbent effectively captured both targets simultaneously from a complex serum matrix. This work highlights the significant potential of bispecific nanobodies as robust and selective ligands for preparing targeted immunoadsorbents. The developed platform represents a superior alternative to non-specific adsorbents, paving the way for more precise and effective extracorporeal therapies against cytokine-driven inflammatory disorders.
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产品关联
DOI
PubMed
高影响因子
Desalination and Water Treatment
·
2016
·
IF 1.272
Jie Cui,Xiaoliang Ma,Xiaogang Wu,Xu Zhang
The adsorption of 2,4,6-trichlorophenol (2,4,6-TCP) on synthesized magnetic mesoporous silica (Fe3O4/SiO2/m-SiO2, MMS) composites and the regeneration of its adsorption capacity through direct UV photolysis was performed in this work. MMS exhibited good performance in removing 2,4,6-TCP from aqueous solutions. The adsorption ratio of 2,4,6-TCP was determined to be influenced by aquatic pH, dissolved humic acids, ionic strength, temperature, and the loaded adsorbents. The adsorption isotherm fit the Freundlich and Polanyi–Manes model better than the Langmuir model, which indicated that the adsorption of 2,4,6-TCP was more likely a pore-filling process. The calculated adsorbed capacity for 2,4,6-TCP on MMS was 55 mg/g. Direct UV photolysis could decompose the adsorbed 2,4,6-TCP and later regenerate the adsorption capacity of MMS to a certain degree; however, the outer mesoporous silica layer was not UV-persistent, and large parts of the mesoporous silica layer were also shown to be corrupted from the Fe3O4/SiO2 core. Therefore, after long duration UV irradiation, the adsorption of 2,4,6-TCP on regenerated MMS was much lower than on freshly synthesized MMS. These results suggest that the photostability of magnetic core-shell-like nanocomposites should be investigated because parts of the composites might leach into the bulk phase during practical use and cause potential environmental risks, similar to other well-known nanomaterials.
研究领域:
环境科学
·
材料科学
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DOI
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
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2013
·
IF 1.684
Xiao-Qiu Dou,Ping Li,Siqing Lu,Xiaobing Tian,Yitian Tang,June D. Mercer-Chalmers,Chuan-Liang Feng,Di Zhang
The highly directional interactions among complex gelators of 2,6-pyridinedicarboxylic acid and 4-hydroxypyridine are investigated, which lead to the formation of ordered one-dimensional fibers with diameters of 500 nm ~ 2 μm. By scanning electron microscopy (SEM), Fourier transform infrared (FT-IR) spectroscopy, 1 H nuclear magnetic resonance ( 1 H NMR) spectroscopy, ultraviolet–visible (UV–vis) spectroscopy, and X-ray powder diffraction (XRD), the co-assembly of the complex into straight fibers under the function of multiple directional hydrogen bonds and π–π stackings is proved. The strong and directional interactions lead to rich dynamic behavior and high degrees of internal order among the complex gelators. The ordered one-dimensional co-assemblies possess useful functions in templated synthesis, molecular recognition , enzyme immobilization, and other biochemistry fields.
研究领域:
化学
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材料科学
·
生物化学
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DOI
高影响因子
CATALYSIS TODAY
·
2013
·
IF 2.98
Xuefeng Hu,Huanhuan Ji,Fei Chang,Yongming Luo
In this study, a rapid reduction of Cr(VI) and degradation of 2,4,6-trichlorophenol (2,4,6-TCP) in a simultaneous manner was reported through the catalysis of g-C 3 N 4 under visible light ( λ > 420 nm) irradiation. The effects of initial concentration of reactants, dissolved O 2 and pH value were investigated systematically. It indicated that, under the optimized concentration, the Cr(VI) reduction and 2,4,6-TCP oxidation could be accomplished in couple of hours in the presence of g-C 3 N 4 . And also, the O 2 involvement and low pH value were able to significantly improve the removal rate of Cr(VI) and 2,4,6-TCP. In addition, the reaction mechanism was investigated through monitoring the reduced states of Cr(VI) and active oxygen intermediates formed during photoreaction by ESR and XPS, as well as determining the degradation products of 2,4,6-TCP by HPLC–MS. The results supported that the redox reactions of Cr(VI) and 2,4,6-TCP can be performed simultaneously via a synergistic oxidation–reduction mechanism in the presence of g-C 3 N 4 under visible light irradiation.
研究领域:
环境科学
·
光催化材料
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DOI
高影响因子
SYNTHESIS-STUTTGART
·
2021
·Vol. 53
·期 14
·2435-2448
·
IF 3.157
Xin Zhou,Qing Long,Dongwei Li,Jingru Gao,Qikai Sun,Shaozi Sun,Yong Su,Peng Wang,Wenjie Peng,Ming Li
Structurally defined and pure oligosaccharides corresponding to β-glucans have attracted great attention because of their potential properties as immunostimulating agents and as antigens of vaccine candidates. We herein describe a convergent synthesis of ready-to-conjugate tridecasaccharides composed of a β-1,3-glucan nonasaccharide backbone and a β-1,6-glucan tetrasaccharide branch. The assembly was achieved by employing trichloroacetimidate glycosylations and features the gram-scale preparation of the nonasaccharide backbone and installation of the tetrasaccharide branch involving orthoester rearrangement to the glycoside.
研究领域:
有机化学
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生物化学
·
疫苗研究
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DOI
高影响因子
KOREAN JOURNAL OF CHEMICAL ENGINEERING
·
2014
·Vol. 32
·期 4
·767-776
·
IF 1.241
Yu Ping,Sun Qilong,Li Jianfeng,Tan Zhenjiang,Pan Jianming,Yan Yongsheng
Magnetic molecularly imprinted microspheres (MMIS) were successfully prepared by suspension polymerization, and then as-prepared MMIS were used as adsorbents for selective recognition of 2,4,6-trichlorophenol (2,4,6-TCP) from aqueous solutions. The results composites were characterized by Fourier transform infrared (FT-IR), X-ray diffraction (XRD), thermo gravimetric analysis (TGA), scanning electron microscope (SEM) and vibrating sample magnetometer (VSM). The results demonstrated that MMIS possesses porous spherical morphology, and exhibits good thermal stability and magnetic property (Ms=10.14 emu g -1 ). Then batch mode of binding experiments was used to determine the equilibrium, kinetics and selectivity recognition. The Langmuir isotherm model fitted the equilibrium data better than did the Freundlich model, and the maximum adsorption capacity on MMIS was about 1.7 times higher than that of MNIS. Kinetics behaviors of MMIS were well described by the pseudo-second-order model. MMIS possessed outstanding selectivity recognition for 2,4,6-TCP in the presence of other competitive phenols (such as sesamol, 3-CP, thymol, 2,4-DCP). Furthermore, the reusability performance of MMIS showed about 17.53% loss after five repeated cycles. Finally, the MMIS were successfully applied to the selective extraction of 2,4,6-TCP from the vegetable samples.
研究领域:
环境科学
·
材料科学
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DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2018
·
IF 6.735
Long Chen,Na Li,Ziyan Wen,Lin Zhang,Qiong Chen,Lina Chen,Pengchao Si,Jinkui Feng,Yanhui Li,Jun Lou,Lijie Ci
A free-standing sandwich-structured polyamide 6 (PA 6)@GO@PA 6 nanofiltration membrane intercalated by nanoparticles with high water flux was prepared using electrospraying combined with electrospinning method. According to the XRD patterns, the interlayer spacing of the membrane increases with the TiO 2 nanoparticle (NP) intercalated. The TiO 2 NP intercalated PA 6@GO (120)@ PA 6 nanofiltration membrane shows a pure water flux up to 13.77 L m −2 h −1 bar −1 even under an extremely low external pressure (1.0 bar), which increased by 80.7% than that of PA 6@GO (120)@PA 6 nanofiltration membrane (7.62 L m −2 h −1 bar −1 ) without nanoparticles intercalated, and maintains high organic dye rejection capabilities (>85% for BF, >92% for MB, >99% for MO, and 99.85% for EB). Besides TiO 2 , other nanoparticles, such as SiO 2 and Si 3 N 4 can also improve the water flux and maintain high rejection rate for MO and MB. Furthermore, TiO 2 intercalated PA 6@GO (120)@PA 6 nanofiltration membrane is very stable even under ultrasonication, and shows a high flux for some common solvents (12.32, 4.91, and 1.26 L m −2 h −1 bar −1 for methanol, ethanol, and NMP, respectively).
研究领域:
材料科学
·
环境工程
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DOI
高影响因子
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
·
2021
·
IF 4.291
Xinyue Jiao,Luminita Marin,Xinjian Cheng
2,4,6-trinitrophenol (TNP) and 2,4-dinitrophenylhydrazine (DNH) are harmful to the environment and human beings. Trace detection of TNP and DNH is worth of developing facile methods. In this work, cellulose powder and filter paper based fluorescence sensor are synthesized. Firstly, NH 2 CH 2 CH 2 – was introduced into cellulose through etherification reaction, and at the same time, a small molecule BODIPY with –CHO was synthesized. Then BODIPY was incorporated into cellulose by Schiff base reaction to obtain fluorescent macromolecular probes. These fluorescent cellulose sensors can efficiently identify TNP and DNH, with the lowest detection limits of 2.8 × 10 −7 M and 1.6 × 10 −7 M, respectively. Fluorescent filter paper is obtained via the same route. They also exhibit TNP and DNH identification ability. The method developed in filter paper not only expands the application range of cellulose, but also can efficiently identify the pollutants TNP and DNH, which is of great significance in theory and practical applications.
研究领域:
环境科学
·
材料科学
·
分析化学
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DOI
高影响因子
ANALYTICAL BIOCHEMISTRY
·
2016
·
IF 2.243
Xiaoxia Shi,Shanshan Sha,Likun Liu,Xin Li,Yufang Ma
Mycobacterium tuberculosis dTDP- d -glucose 4,6-dehydratase (RmlB) is the second enzyme for the biosynthesis of dTDP- l -rhamnose, which is a sugar donor to the synthesis of the cell wall linker, d - N -acetylglucosamine- l -rhamnose. RmlB is essential to mycobacterial growth and is not found in humans; therefore, it is a potential target for developing new anti-tuberculosis drugs. So far, there has been no suitable method for high-throughput screening of RmlB inhibitors. Here, the recombinant M. tuberculosis RmlB was purified and an absorbance-based microtiter plate assay was developed for RmlB activity. It could be used for high-throughput screening of RmlB inhibitors. The kinetic properties of M. tuberculosis RmlB, including optimal pH, optimal temperature, the effect of metal ions , and the kinetic parameters , were determined with this assay. The inhibitory effects of dTTP and dTDP on M. tuberculosis RmlB were also studied with the assay.
研究领域:
微生物学
·
药物开发
·
生物化学
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DOI
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
·
2014
·Vol. 131
·期 22
·
IF 1.64
Yanyan Zhang,Hong Fan,Bo-Geng Li
Advance polyamide-6- b -polydimethylsiloxane (PA6- b -PDMS) multiblock copolymers were first synthesized via the polymerization in bulk. Binary carboxyl terminated PA6 was served as the hard segment and PDMS modified with hexamethylene diisocyanate (PDMS-NCO) was the soft segment. A series of PA6 -b- PDMS copolymers based on different content and length of soft segments were obtained. Interestingly, Differential scanning calorimetry (DSC) studies revealed no obvious change in melting temperature after introducing PDMS segments to copolymers. The high melting temperatures indicated these copolymers possess potential applications in automotive industry that require high continuous use temperatures. DSC and transmission electron microscopy studies both demonstrated increasing the length and the content of the soft segment contributed to increasing of the degree of microphase separation. However, the improvement of thermal stability resulting from PDMS segments was also observed by thermo gravimetric analysis. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014 , 131 , 41114.
研究领域:
材料科学
·
高分子化学
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DOI
高影响因子
Journal of Saudi Chemical Society
·
2021
·
IF 3.932
Smaher M. Elbayomi,Xuan Nie,Ye-Zi You,Tamer M. Tamer
Cationic polymers with high charge density could effectively condense the DNA and achieve gene transfection; however, it often brings non-negligible cytotoxicity. Notably, the high charge density gene vector fails in the serum environment, limiting further application in vivo. In this paper, an efficient and reliable non-viral gene vector of poly (amidoamine) (PAA) was designed by introducing diacryolyl-2,6-diaminopyridine (DADAP) onto the PAA backbone through Michael-addition polymerization, which provides high transfection efficiency in a serum-containing environment. Diacryolyl-2,6-diaminopyridine and cationic parts provided multiple interactions between gene vectors and DNA, including hydrogen bond and electrostatic interactions. The introduction of hydrogen bonding can effectively reduce the charge density of polyplexes without reducing the DNA condensing ability, incorporating the diaminopyridine group and cationic part in PAA chains successfully consolidated cellular uptake, endosome destabilization, and transfection efficiency for the PAA/DNA complexes with low cytotoxicity. The constructed vector with multiple interactions presented 6 times higher transfection efficiency in serum-free and 9 times in serum-containing environment than that of branched polyethyleneimine (PEI 25K) in 293T cells in vitro. Therefore, introducing the hydrogen band to form low charge density polyplexes with high transfection efficiency and low cytotoxicity has a great potential in gene delivery.
研究领域:
生物化学
·
分子生物学
·
基因治疗
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DOI
高影响因子
危险品化学品经营许可证（带存储）