FOOD CHEMISTRY
·
2023
·
IF 8.8
Mengyang Hou,Chengyuan Lin,Yanhua Ma,Jingchun Shi,Jie Liu,Lin Zhu,Zhaoxiang Bian
Chaenomeles speciosa (Sweet) Nakai fruit is a good source of phenolics with many health benefits. In this work, the enrichment of C. speciosa fruit total phenolics (CSFTP) using macroporous resins was studied. NKA-Ⅱ resin was selected for enriching CSFTP due to its highest adsorption/desorption quantity. Adsorption characteristics of CSFTP on NKA-Ⅱ resin exhibited a good fit with the Langmuir isotherm model and pseudo-second order kinetics model. This adsorption was spontaneous, exothermic, and entropy-decreasing through a physisorption mechanism. The breakthrough-elution curves were studied to optimize CSFTP enrichment conditions. One-step enrichment increased CSFTP content in the extracts from 26.51 % to 78.63 %, with a recovery of 81.03 %. A UPLC-QqQ-MS/MS method in multiple reaction monitoring (MRM) mode was established and validated for the simultaneous quantification of seven phenolic compounds. This study demonstrates the feasibility of industrial enrichment of CSFTP using NKA-Ⅱ resin and proposes a reliable method for quality control of CSFTP-rich products.
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高影响因子
Analytical Methods
·
2023
·Vol. 15
·期 41
·5492-5499
·
IF 3.1
Xingle Guo,Haijuan Jiang,Yuqi Guo,Liyan Jia,Xu Jing,Junxue Wu
A novel method based on homogeneous liquid–liquid extraction with deep eutectic solvents (DES) under subzero-temperature conditions in combination with high performance liquid chromatography (HPLC) for the determination of chiral fungicide triadimefon (TF) and its metabolite triadimenol (TN) in water, fruit juice, vinegar, and fermented liquor was developed in this study. The method involved using deep eutectic solvents (DES) under subzero-temperature conditions in combination with high performance liquid chromatography (HPLC). This novel technique, known as subzero-temperature homogeneous liquid–liquid extraction (STHLLE), offers several advantages, including high efficiency, time-saving, low-cost, and eco-friendliness. The enantiomers of chiral TF and TN were simultaneously separated and quantified using HPLC coupled with a Daicel Chiralpak OD-RH column. Various experimental parameters such as DES composition and volume, freezing condition, salt concentration, and pH were optimized to enhance the recoveries of the target analytes. Under the optimized conditions, spiked recoveries of six enantiomers (i.e., S-TF, R-TF, SR-TN, RS-TN, SS-TN, and RR-TN) in the water, fruit juice, vinegar, and fermented liquor samples were 82.2–100.1% with relative standard deviations of 0.4–10.1%. The current method demonstrated a detection range of 0.03–0.06 mg L−1 in the target analytes. This established technique exhibits potential for efficient and precise extraction and quantification of the enantiomers of TF and TN in water phase samples.
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Food & Function
·
2023
·Vol. 14
·期 20
·9295-9308
·
IF 6.1
Yin Qin,Ruyan Fan,Yingxin Liu,Shuyi Qiu,Lu Wang
Nonalcoholic fatty liver disease (NAFLD), as the commonest chronic liver disease, is accompanied by liver oxidative stress and inflammatory responses. Herein, the extract obtained from Rubus corchorifolius fruits was purified and characterized for its polyphenol composition. The liver protective effect of the purified R. corchorifolius fruit extract (RCE) on mice with high-fat-diet (HFD)-induced NAFLD were investigated, and the potential mechanisms were explored through the integration of transcriptomics and metabolomics. Results showed that the polyphenolic compounds in RCE mainly included (−)-epigallocatechin, procyanidin B2, keracyanin, vanillin, dihydromyricetin, and ellagic acid. In addition, RCE intervention ameliorated liver and mitochondrial damage, which was evidenced by decreased indices of oxidative stress, liver function markers, and lipid profile levels. The liver metabonomics research revealed that RCE intervention affected the metabolic pathways of metabolites, including linoleic acid metabolism, galactose metabolism, alanine, aspartate and glutamate metabolism, retinol metabolism, glycine, serine and threonine metabolism, tryptophan metabolism, aminoacyl-tRNA biosynthesis, riboflavin metabolism, starch and sucrose metabolism, and arachidonic acid metabolism. Additionally, liver transcriptomics research indicated that pathways like fatty acid degradation, circadian rhythm, valine, leucine and isoleucine degradation, primary bile acid biosynthesis, cytokine–cytokine receptor interaction, adipocytokine signaling pathway, glutathione metabolism, lipid and atherosclerosis were significantly enriched. The transcriptomics and metabolomics analysis demonstrated that RCE intervention had significant modulatory effects on the metabolic pathways associated with glycolipid metabolism. Moreover, RT-PCR results verified that RCE intervention regulated liver mRNA levels associated with the inflammatory response. Therefore, our findings suggest that the intake of RCE might be an effective strategy to alleviate liver damage.
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FOOD CHEMISTRY
·
2023
·
IF 8.8
Liangrui Lv,QingQing Chen,Chunyang Jing,Xiaoying Wang
To sensitively monitor trace-level of toxic patulin (PAT), an ultrasensitive PAT ratiometric aptasensor based on the dual-potential electrochemiluminescence (ECL) of Ru(bpy) 3 2+ was first proposed. Noteworthily, Ru(bpy) 3 2+ -doped trimetallic nanocube ( [email protected] ) innovatively integrated the luminophore and cathode coreaction accelerator (CCA), which could generate strong cathodic ECL in the existence of low concentration of K 2 S 2 O 8 . Simultaneously, anthocyanin-derived carbon quantum dots (anth-CQDs) prepared from purple potato skins was first served as a green anodic coreactant. And SiO 2 -coated anth-CQDs ( [email protected] 2 ) exhibited excellent performance for enhancing anodic ECL of [email protected] Based on this, a novel ternary ECL system was established. In the presence of PAT, the ECL intensity ratio of anode to cathode ( I ECL-A / I ECL-C ) was significantly increased, and a low detection limit of 0.05 pg mL −1 was obtained. Moreover, when proposed method and high performance liquid chromatography (HPLC) were simultaneously applied to series of fruit products, the obtained results were completely consistent, reflecting its practicability.
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FOOD CHEMISTRY
·
2019
·
IF 5.399
Chan-Yuan Yao,Zhen-Lin Xu,Hong Wang,Fan Zhu,Lin Luo,Jin-Yi Yang,Yuan-Ming Sun,Hong-Tao Lei,Yuan-Xin Tian,Yu-Dong Shen
Alternariol (AOH), a secondary metabolites of the genus Alternaria , is a genotoxic mycotoxin. Because the previous “Mannich antigen” for AOH readily underwent unstable induction, a new AOH hapten was designed for inducing a high-quality antibody against AOH using a conjugate of the AOH carboxymethyl derivative with a carrier protein as the immunogen. A competitive indirect chemiluminescence enzyme immunoassay (ciCLEIA) for AOH was optimized and showed good sensitivity (limit of detection 0.068 ng/mL), a linear range (0.11–1.23 ng/mL), and negligible cross-reactivity with analogues. Average rates of recovery from spiked samples (juice and cereal) were between 72.7% and 115.8%, with coefficients of variation <14.3%. Results from ciCLEIA correlated well with those of high-performance liquid chromatography coupled with tandem mass spectrometry, meaning the proposed method might be an effective screening tool for detection of AOH in food samples.
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Food Analytical Methods
·
2014
·Vol. 8
·期 6
·1556-1564
·
IF 1.802
Liu Tao,Zhang Xiao,Hao Jianpeng,Zhu Wenxin,Liu Wei,Zhang Daohong,Wang Jianlong
Herein, we for the first time report an acetylcholinesterase (AChE)-free colorimetric method for the detection of chlorpyrifos (CP), which is based on the oxidation reaction of H 2 O 2 with CP and 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS 2− ) catalyzed by hemin/G-quadruplex DNAzyme. In the absence of CP, ABTS 2− is directly oxidized by H 2 O 2 with hemin/G-quadruplex DNAzyme as a biocatalyst to produce the blue-green-colored free radical anion (ABTS ·− ), accompanied with an obvious UV-visible absorbance at 418 nm. However, the amount of H 2 O 2 used in H 2 O 2 -DNAzyme-ABTS 2− system will be decreased if CP is first reacted with H 2 O 2 , leading to the decrease of the amount of ABTS ·− and a change in the UV-visible absorbance of ABTS ·− at 418 nm. Thus, an indirect dependence relation between CP concentration and the absorbance of ABTS ·− at 418 nm could be constructed and used for CP detection. Under optimal conditions, the detection range of the method is 0.04–1 μg/mL, with a detection limit of 0.01 μg/mL. Application of the method in real juice samples shows good analytical features in terms of recoveries (95–107.5 %), the relative standard deviation (RSD) (0.6–4.7 %), rendering it as an attractive candidate to current methodologies for the determination of CP.
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MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 3
·1-12
·
IF 5.3
Wang Hongjun,Bian Ziyang,Wang Yue,Yang Zhenshan,Niu Huijuan,Li Hefu
A dual-functional biomimetic surface-enhanced Raman scattering (SERS) substrate composed of grating/microcavity arrays (GCA) was developed for on-site monitoring of pesticides on fruit peels and in surface water. The hierarchical GCA structure was fabricated by molding a negative polydimethylsiloxane (PDMS) replica from lily petals, followed by the generation of grating wrinkles through oxygen plasma treatment. After the deposition of silver nanoparticles (Ag NPs) through magnetron sputtering, the optimal Ag NPs@GCA substrate exhibits high sensitivity, good uniformity, and reproducibility. Moreover, due to its optical transparency, mechanical stability, and hydrophobic properties, the Ag NPs@GCA substrate combined with a handhold Raman spectrometer can be used for on-site determination of pesticides in real samples. The limit of detection (LOD) for thiram on the surfaces of apples, pears, and cherry tomatoes were 2.86 × 10 −7 , 5.03 × 10 −7 , and 7.25 × 10 −7 M, respectively. For malachite green, the LODs were 9.04 × 10 −8 M in lake water and 4.83 × 10 −7 M on fish scales. The results indicate the developed dual-functional Ag NPs@GCA substrate has great potential for applications in food safety assessment and environmental pollutant monitoring. Graphical abstract
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POSTHARVEST BIOLOGY AND TECHNOLOGY
·
2025
·
IF 6.8
Zhimin Wang,Mingming Huang,Mei Dai,Dandan Tian,Yingying Shi,Bin Wu,Zheng Zhang,Zunyang Song,Jingying Shi
Peach fruit is highly susceptible to decay caused by Botrytis cinerea . Volatile compounds have attracted considerable attention due to their safety and practical application advantages. S-methyl butanethioate, a volatile organic compound, is biosynthesized by microorganisms and plants. In this study, fumigation with synthetic s-methyl butanethioate at 50, 100, 200 and 300 μL/L effectively inhibited mycelial growth and conidial germination of B. cinerea and disease incidence of gray mold on peach fruit in a concentration-dependent manner. Furthermore, s-methyl butanethioate induced conidial apoptosis by causing the accumulation of reactive oxygen species (ROS) and exacerbating mitochondrial damage. To address the limitations in solubility, stability, and volatility of s-methyl butanethioate, a slow-release fresh-keeping card was prepared by embedding s-methyl butanethioate into β-cyclodextrin. Scanning electron microscopy (SEM) and Fourier transform-infrared spectroscopy (FT-IR) confirmed the successful embedding of s-methyl butanethioate into the cavity of β-cyclodextrin, forming the encapsulated structure of the inclusion complex. Notably, the release of s-methyl butanethioate from the fresh-keeping card was delayed for 15 d and the antifungal effect was stronger than direct fumigation with s-methyl butanethioate. Moreover, the fresh-keeping card application prevented surface damage to peach fruit caused by direct fumigation. Therefore, the fresh-keeping card loaded with encapsulated s-methyl butanethioate not only enhanced stability but also extended antifungal efficacy, outperforming the traditional un-encapsulated form. Therefore, this study demonstrates that fresh-keeping card loaded with natural components might serve as an alternative to synthetic chemical fungicides for controlling postharvest diseases of fruit and vegetables.
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LWT-FOOD SCIENCE AND TECHNOLOGY
·
2024
·
IF 6
Chao Zhang,Chunhe Gu,Fan Su,Mengrui Wang,Junxia Chen,Ziqing Chang,Junping Zhou,Mingzhe Yue,Fei Liu,Zhen Feng
Iridoid glycosides (IGs) are secondary metabolites found in plants such as the noni plant ( Morinda citrifolia L.), known for their diverse biological activities and potential health benefits. Herein, we screened fifteen natural deep eutectic solvents (NADES) and two conventional solvents (CS, 70% (V/V) methanol and 70% (V/V) ethanol) for extraction of IGs from noni fruit pomace. Our findings indicate that non-acidic NADES promote IG extraction. Glycerol/glycine combined with β-cyclodextrin was selected, and a molecular simulation was performed to elucidate the interaction between NADES components and IGs. Further, the structure-activity relationship and interaction mechanisms of IGs with α-glucosidase were investigated using multispectral and molecular docking tools. The results demonstrated that deacetylation of the B-ring's C-10 improved the inhibitory potential of IGs. Deacetylation produces hydrophobic contacts, which enhance the inhibitory effect of deacetylasperulosidic acid on α-glucosides. Additionally, hydrogen bonds and van der Waals forces drive the formation of non-covalent complexes between IGs and enzymes.
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Microbiology Spectrum
·
2024
·
IF 3.7
Yun Li,Ruifeng Ma,Chenying Gao,Ziyi Li,Yongqin Zheng,Fang Fang,Cheng Wang,Guohua Li,Xiaozhen Du,Changbao Xu,Meirong Xu,Rui Liu,Xiaoling Deng,Zheng Zheng
“Candidatus Liberibacter asiaticus” (CLas), the causal agent of citrus Huanglongbing (HLB), is able to multiply to a high abundance in citrus fruit pith. However, little is known about the biological processes and phytochemical substances that are vital for CLas colonization and growth in fruit pith. In this study, CLas-infected fruit pith of three citrus cultivars (“Shatangju” mandarin, “Guanxi” pomelo, and “Shatian” pomelo) exhibiting different tolerance to CLas were collected and used for dual RNA-Seq and untargeted metabolome analysis. Comparative transcriptome analysis found that the activation of the CLas noncyclic TCA pathway and pathogenic-related effectors could contribute to the colonization and growth of CLas in fruit pith. The pre-established Type 2 prophage in the CLas genome and the induction of its CRISPR/cas system could enhance the phage resistance of CLas and, in turn, facilitate CLas population growth in fruit pith. CLas infection caused the accumulation of amino acids that were correlated with tolerance to CLas. The accumulation of most sugars and organic acids in CLas-infected fruit pith, which could be due to the phloem blockage caused by CLas infection, was thought to be beneficial for CLas growth in localized phloem tissue. The higher levels of flavonoids and terpenoids in the fruit pith of CLas-tolerant cultivars, particularly those known for their antimicrobial properties, could hinder the growth of CLas. This study advances our understanding of CLas multiplication in fruit pith and offers novel insight into metabolites that could be responsible for tolerance to CLas or essential to CLas population growth.
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FOOD HYDROCOLLOIDS
·
2026
·
IF 12.4
Zitian Liu,Ligang Lin,Jiaxin Zhang,Kui Niu,Minglun Cheng,Yuxuan Zhu,Bin Du,Wenlong Hou,Huiyang Shang
Natural biopolymer food packaging materials face limitations in practical applications due to insufficient packaging performance and functional activity. To address these drawbacks, this study developed a strategy combining film matrix modification with synergistic active ingredient introduction to effectively enhance physicochemical properties and functional activity of polysaccharide-based films. Specifically, silver-based meta-organic frameworks (SA/Ag-2MI) were synthesized within sodium alginate, enabling it to serve as both a film-forming matrix and a functional component. Subsequently, curcumin-cyclodextrin inclusion complexes (CUR@CD) were incorporated as active additives into the film-forming matrix composed of SA/Ag-2MI, guar gum and carboxylated cellulose nanofibers, further enhancing the film's physicochemical properties and functional activity. Compared to the pristine film, GSC/Ag-2MI/CUR@CD film exhibited superior water vapor barrier property (water vapor permeability decreased by 22.98 %), light barrier property (transmittance at 600 nm decreased by 30.24 %), antioxidant activity (scavenging rate for two free radicals increased to over 50 %) and antibacterial active (inhibition zone appearance of three bacteria). Furthermore, the GSC/Ag-2MI/CUR@CD film effectively maintained strawberry storage quality by significantly inhibiting appearance changes, weight loss, firmness reduction and rot occurrence. In summary, this work offers a promising and feasible pathway for enhancing the performance of natural biopolymer packaging, with considerable potential in active packaging and fruit preservation fields.
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JOURNAL OF SEPARATION SCIENCE
·
2019
·Vol. 42
·期 7
·1432-1439
·
IF 2.516
Ruiyu Liang,Yi Peng,Yuling Hu,Gongke Li
A novel hybrid triazine-imine core-shell magnetic covalent organic polymer with high adsorption capacity and excellent stability was fabricated by surface-assisted in situ growth technique. The composite possesses porous and extended π-conjugated system, and was applied as the magnetic sorbent for efficient enrichment and rapid separation of pesticides. A new analytical method for simultaneous determination of eight pesticides in fruit samples was developed by magnetic solid phase extraction combined with ultra high performance liquid chromatography and tandem mass spectrometry. The effect of extraction time, desorption time, and the type of desorption solvent on the extraction efficiency were evaluated. The established method shows good repeatability and high sensitivity. The repeatability of this method was estimated with relative standard deviations in the range of 0.7–7.0% ( n = 5) for the same batch, and 1.7–10% ( n = 3) for batch to batch. Good linearity for eight pesticides was obtained with coefficient of determination in the range of 0.9942–0.9990. Limit of detections ranged from 0.4 to 1.2 ng/L. Real sample determination showed that four and two pesticides were detected in strawberry and grape, respectively. The results demonstrated that the established method was efficient, sensitive, and convenient for trace determination of pesticides in fruit samples.
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危险品化学品经营许可证（带存储）