BIOSENSORS & BIOELECTRONICS
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2017
·
IF 7.78
Zhiyong Tang,Junlin He,Jun Chen,Yazhen Niu,Yilin Zhao,Yuchan Zhang,Chao Yu
We are presenting an electrochemical immunosensor for the determination of Galectin-3 (Gal-3), a biomarker of heart failure. A glassy carbon electrode (GCE) was modified with a film of a composite made from the N-doped graphene nanoribbons immobilized Fe-based-Metal-organic frameworks deposited with Au nanoparticles (N-GNRs-Fe-MOFs@AuNPs). Primary antibody against Gal-3 (Gal-3-Ab 1 ) was immobilized on the Au nanoparticles on the surface of the modified GCE which then was blocked with bovine serum albumin . Signal amplification is crucial for obtaining low detection limits in biosensors. Here, a relatively simple and ultrasensitive sandwich-type electrochemical immunosensor based on novel signal generation and amplification was developed for the determination of Gal-3. A kind of novel redox-active species, AuPt-Methylene blue (MB) (AuPt-MB) nanocomposites , was synthesized by a one-pot method for the first time. Wherein, MB as a kind of the electron transfer mediators in an amperometric biosensor is responsible for electron production and signal amplification. The rod-like AuPt-MB nanohybrids displayed uniform morphology and good electrochemical activity and can combine with the second antibodies against Gal-3 (Gal-3-Ab 2 ). And the AuPt-MB-Ab 2 coupled with the N-GNRs-Fe-MOFs@AuNPs-Ab 1 to form the sandwich type format that can greatly enhance the biosensor's sensitivity. Under optimal conditions, the designed immunosensor exhibited a linear concentration range from 100 fg mL −1 to 50 ng mL −1 , with a low detection limit of 33.33 fg mL −1 (S/N = 3) for Gal-3 in spiked serum. Additionally, the designed immunosensor showed acceptable selectivity, reproducibility and stability. The satisfactory results in analyzing human serum samples indicated potential application promising in monitoring biomarkers.
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FOOD CHEMISTRY
·
2025
·
IF 9.8
Haiyan Zhao,Yunzhi Zhao,Shanshan Li,Meiya Zhang,Na Li,Min Cui,Cong Zhang
Kanamycin (KAN), a veterinary antibiotic, often leads to residue pollution in food, thereby threatening human health. Therefore, we established a dual-signal ratiometric electrochemical aptasensor for KAN detection in food. Herein, a ferrocene-functionalized COFs-derived Fe/N-doped porous carbon was synthesized and served as splendid electrode material to generate amplified electrocatalytic signal toward hydroquinone (HQ). Tetrahedral DNA-supported aptamer probes were employed to improve binding affinity and reduce nonspecific adsorption. Methylene blue-enriched COFs (MB@SA-COFs) were designed as another signal tag to generate an effective MB signal. With the recognition of KAN, the MB@SA-COFs would detach from electrode surface, inducing a decreased MB signal and decreased resistance, thereby causing an increased HQ signal. The signal on-off mode was conducive to mitigating environmental interference through self-calibration. Consequently, the aptasensor showed a wide linear range and a low detection limit for KAN detection. Satisfactory applicability was observed in milk samples, demonstrating its practicability for food safety monitoring.
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ELECTROCHIMICA ACTA
·
2022
·
IF 7.336
Minling Ding,Huizhe Niu,Nan Zhang,Tongtong Hou,Ping Guan,Xiaoling Hu
Here we report on a facile, rapid, selective, and stable electrochemical sensing platform for nicotinamide adenine dinucleotide (NADH) based on polythionine-methylene blue/electrochemically reduced graphene oxide modified glassy carbon electrode (PTH-MB DES -ERG/GCE). The ERG was directly formed on a glassy carbon electrode (ERG/GCE) through one-step electrochemical reduction of GO in PBS solution (10 mM, pH 7.4). In a PBS electrolyte, respectively, containing 50% (v/v) three different deep eutectic solvents (DES), thionine and methylene blue were polymerized on the surface of ERG/GCE. The electrochemical properties of the polymers polymerized in different electrolytes were investigated. The PTH-MB DES -ERG composite has a higher performance in the electrocatalytic oxidation of NADH than that of PTH-MB DES . The as-prepared PTH-MB DES -ERG/GCE demonstrated excellent direct electrocatalytic oxidation toward NADH, providing a favorable platform for electron transfer from NADH to the electrode. The sensor displayed a wide linear range from 1.52 μM to 3333.33 μM with a limit of detection of 0.51 nM. The interference from the ascorbic acid and uric acid was negligible. The prepared sensor was further tested for the determination of NADH in artificial urine samples, showing the PTH-MB DES -ERG/GCE formed by electropolymerization have promising biomedical applications.
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ACS Applied Polymer Materials
·
2023
·
IF 5
Kunling Liu,Mengmeng Fu,Xue Ma,Pengcheng Zhang,Shuai Zong,Wenjie Zhao
Methylene blue (MB) is extensively used as a coloring agent in the textile and pharmaceutical industries, causing ecological risk and physical defunctions. Unfortunately, the adsorbents commonly used to remove MB from the environment are also limited by disadvantages, such as harsh synthesis conditions and poor adsorption capacity. In this paper, we present a simple and low-cost method for preparing carboxyl-functionalized hyper-cross-linked microporous polymers (HCPTPB-COOH) capable of efficiently removing MB from water. To reduce chemical reagent consumption and improve productive efficiency, aluminum trichloride was employed as a catalyst for both Friedel-Craft alkylation and Friedel-Craft acylation reactions; meanwhile, dichloromethane served the dual function of solvent and cross-linker reagent. The successful synthesis of HCPTPB-COOH was confirmed by clearly characterizing its chemical structure, morphology, and pore distribution. Due to its rich pore structure (Brunauer–Emmett–Teller (BET) = 720.2 m2 g–1) and multiple interactions, including hydrophobic interactions, π–π stacking, hydrogen bonding, and electrostatic interactions, the developed HCPTPB-COOH exhibited excellent adsorption capacity for MB compared to most traditional adsorbents. In particular, the organic contaminants were efficiently adsorbed from the aqueous solution despite the small amount of adsorbent and the presence of other ionic species, and they also exhibited excellent reusability with a minimal decline in adsorption performance after at least six adsorption cycles. Adsorption isotherms demonstrated that HCPTPB-COOH was more consistent with the Langmuir adsorption model, with a maximum adsorption of 371 mg g–1 for MB. The adsorption kinetics indicated that the pseudo-second-order dynamics model was more compatible with MB. The presence of negative Gibbs free energy change indicated that the adsorption process was spontaneous.
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LANGMUIR
·
2024
·
IF 3.7
Liyun Zhang,Xin Wen,Guilan Zhang,Xin Wang,Xiang Li,Cong Peng,Minghang Jiang,Mengjun Wang,Lan Ma
Developing a strong catalytic antifouling membrane to achieve efficient sewage purification has great potential for alleviating water crisis. In this work, we designed and prepared an Fe/Cu-layered double hydroxide (Fe–Cu LDH)-coated polyvinylidene fluoride (PVDF) composite membrane (PVDF/Fe–Cu LDHs) with strong antifouling and activating peroxymonosulfate (PMS) catalytic degradation performance through polydopamine-coordination anchoring and hydrothermal reaction. The results showed that abundant hydroxyl groups of the LDH surface endowed the superhydrophilicity (water contact angle <10°) and underwater superoleophobicity (underwater–oil contact angle >150°) of the membrane surface, which displayed outstanding resistance to crude oil adhesion. With assistance of the LDH surface-bound sulfate radical of the peroxymonosulfate system, the PVDF/Fe–Cu LDH membrane demonstrated robust catalytic degradation performance for the methylene blue (MB) in the dark; the degradation rate constant (k, min–1) reached 0.96. Meanwhile, facing the oily wastewater, the selective wettability and charge effect of LDH of the surface made the PVDF/Fe–Cu LDH membrane realize the separation for the various surfactant-free and surfactant-stabilized emulsions. Importantly, the PMS-activation catalytic produced the ROS (•SO4–,•OH, •O2–, and 1O2), which enhanced the regeneration of the fouled PVDF/Fe–Cu LDH membrane and obtained a high flux recovery ratio in the dark (94.7%) after 10 cycles of separation experiments. Hence, we believed that the PVDF/Fe–Cu LDH membrane can provide inspiration for the development and further practical application of antifouling membranes.
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JOURNAL OF CHEMICAL AND ENGINEERING DATA
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2019
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IF 2.298
Rong Wo,Qiu-Lin Li,Chao Zhu,Yan Zhang,Ge-fei Qiao,Kai-yu Lei,Ping Du,Wei Jiang
In this work, a novel core@double-shell three-layer structure adsorbent (Fe3O4@SiO2@Zn–TDPAT) [TDPAT = 2,4,6-tris(3,5-dicarboxyl phenylamino)-1,3,5-triazine] was prepared to remove congo red (CR) and methylene blue (MB). The as-obtained magnetic adsorbent Fe3O4@SiO2@Zn–TDPAT was characterized by X-ray diffraction, Fourier transform infrared spectra, vibrating sample magnetometry, transmission electron microscopy, and scanning electron microscopy. The influencing adsorption parameters including adsorbent amounts, extraction time, pH, and ionic strength were investigated. Under the optimized conditions, the removal efficiencies of CR and MB reached 100 and 99%, respectively. The pseudo-second-order kinetic model and Langmuir model were suitable to illustrate the adsorption processes of CR and MB. The thermodynamic values, including Gibbs free energy (ΔG°), enthalpy (ΔH°), and entropy (ΔS°), showed that the adsorption processes were spontaneous and endothermic in nature. Moreover, the obtained nanocomposite remained stable in different acid–alkaline solutions and exhibited good reusability after multiple cycles. All of the experimental results indicated that Fe3O4@SiO2@Zn–TDPAT could be a potential adsorbent to purify dye wastewater.
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RSC Advances
·
2019
·Vol. 9
·期 58
·33872-33882
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IF 3.049
Genxing Zhu,Qi Liu,Fengyi Cao,Qi Qin,Mingli Jiao
Dyes are typical water contaminants that seriously affect water quality. In this study, silkworm cocoon derived N, O-codoped hierarchical porous carbon was successively developed via a facile pre-carbonization and chemical activation method, and characterized thoroughly by SEM, TEM, HRTEM, XRD, Raman, N2 adsorption and XPS. The as-prepared N, O-HPC showed a well-developed porous structure with an ultra-high specific surface area of 2270.19 m2 g−1, which proved to be a high-efficiency adsorbent. Batch adsorption experiments demonstrated that MB adsorption was highly dependent on contact time, initial MB concentration, temperature and initial solution pH. However, no remarkable effects of humic acid and ionic strength were observed. In the kinetic studies, the good applicability of a pseudo-second-order kinetic model was demonstrated. The adsorption isotherm study showed that a Langmuir isotherm model can describe the experimental data much more suitably with a maximum monolayer adsorption capacity value of 2104.29 mg g−1, which is among the highest in previously reported adsorbents and ascribed to multiple adsorption mechanisms including pore filling, π–π stacking interaction and electrostatic interaction between MB and N, O-HPC. Thermodynamic analyses suggested that MB adsorption onto N, O-HPC was spontaneous and endothermic. Furthermore, the as prepared adsorbent showed highly efficient adsorption for MB in tap water and synergistic adsorption performance toward MB and MO. Therefore, N, O-HPC derived from silkworm cocoon could be considered as an efficient, novel and advantageous material for wastewater remediation.
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JOURNAL OF MOLECULAR LIQUIDS
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2024
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IF 5.3
Tianqi Liu,Hailian Huang,Yunzong Liu,Konstantinos Papadikis,Beata Layla Mehdi,Pow-Seng Yap,Graham Dawson
A novel magnetic quinary nanocomposite, protonated g-C 3 N 4 /Fe 3 O 4 /AgBr/AgCl/AgI, was synthesized using a wet chemical method. The multi-heterojunction photocatalyst with multi carrier source and multi-channel carrier transport is constructed to enhance the photocatalytic degradation performance of pure g-C 3 N 4 . The design of the quinary nanocomposite is also an effective approach solving the chemical stability problem of silver halides. The photocatalytic degradation efficiency under visible light irradiation was analyzed for methylene blue (MB) as the target organic pollutant. The quinary nanocomposite with 10 % g-C 3 N 4 displayed high photocatalytic performance, with 83 % of MB degraded under 6 h LED white light irradiation. The effective quinary nanocomposite has good magnetic properties and can be magnetically separated to prevent secondary pollution of the photocatalyst in water. The band gap of protonated g-C 3 N 4 /Fe 3 O 4 /AgBr/AgCl/AgI was found to be lower than pure g-C 3 N 4 . Parameters such as initial concentration of pollutant, initial solution pH, and light source were studied. Zeta potential results showed that the quinary nanocomposite could disperse homogenously to decrease aggregation in the solution. The photocatalytic mechanism was investigated, and the mineralization results were analyzed based on liquid chromatography-mass spectroscopy (LC-MS) and total organic carbon (TOC). It was found that the hydroxyl and superoxide radicals were major reactive species by scavenging experiments. Additionally, protonated g-C 3 N 4 /Fe 3 O 4 /AgBr/AgCl/AgI nanocomposite showed excellent reusability after magnetic separation and regeneration, thus suggesting it to be a promising photocatalyst for treating wastewater containing organic contaminants.
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ACS Applied Optical Materials
·
2023
·
IF 0
Xiaoya Bu,Yan-gai Liu,Ci’an Xie,Baochen Wang
The long-afterglow luminescent material Sr2MgSi2O7:(Eu, Dy) was combined with the photocatalytic material g-C3N4 to construct a type of Sr2MgSi2O7:(Eu, Dy)/g-C3N4 self-luminous photocatalytic composite. This overcomes the limitation that photocatalytic experiments can only be carried out under an external light source, and it provides a more flexible way to use photocatalytic technology in environmental purification. The morphology, structure, luminescence, and photocatalytic properties of the samples were investigated using a variety of advanced characterization techniques. The results show that the significant improvement in the photocatalytic performance is attributed to the formation of a Z-scheme heterojunction between the luminescent and photocatalytic materials and the promotion of the photocatalytic reaction by the properties of the long-afterglow luminescent material itself. A possible mechanism for the photocatalytic process of the composite is proposed, and it is speculated that the reason for the increased photocatalytic activity is internal electron transfer and electron–hole pair separation based on tests such as X-ray photoelectron spectroscopy and density functional theory calculations. This study provides a strategy for enhancing the photocatalytic activity of photocatalysts and achieving round-the-clock degradation of pollutants.
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CHEMOSPHERE
·
2023
·
IF 8.8
Jida Gao,Qianji Lin,Tingzhi Yang,Yong chao Bao,Juan Liu
Coal gangue is a kind of solid waste produced in the process of coal mining and washing. Its silicon aluminum silicon aluminum oxide content is high, respectively, which are suitable for resource utilization as raw materials for Si–Al molecular sieving. In this paper, a novel, simple, low-cost, and environmentally friendly process was carried out to prepare ZSM-5 zeolite by solvent free method after calcination, acid leaching, and alkali melting. The obtained samples were characterized by Energy Dispersive Spectrometer (EDS), Inductively Coupled Plasma (ICP), Thermo-gravimetry Analysis (TG), X-ray Photoelectron Spectroscopy (XPS), Fourier Transform Infrared Spectrometer (FTIR) X-Ray Diffraction (XRD), Scanning Electron Microscope (SEM) and N 2 adsorption isotherm. The characteristics of the raw materials and the adsorption mechanism of the prepared samples were characterized. Through a series of pretreatment such as calcined acid leaching and alkali melting of the raw materials, the silicon-aluminum ratio of the sample reaches 1.749, and the maximum specific surface area of the sample can reach 252.59 m 2 /g. The obtained samples were used to adsorb heavy metal ions and methylene blue solution, and the removal rate of lead ions and methylene blue solution was more than 95%. The theoretical maximum adsorption capacity of Pb ion, methylene blue solution and copper ion can reach 232.56 mg/g and 118.34 mg/g. The adsorption process is mainly chemical adsorption. The product could be suitable for removing both heavy metal ions and cationic dyes from the wastewater and had broad application prospects.
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JOURNAL OF HAZARDOUS MATERIALS
·
2021
·
IF 10.588
Kunyi He,Tong Bu,Xiaohan Zheng,Junfang Xia,Feier Bai,Shuang Zhao,Xin yu Sun,Mengna Dong,Li Wang
The sensitive detection of foodborne pathogens is of great significance for ensuring food safety and quality. Herein, on the basis of methylene blue-embedded zirconium based organic framework ( [email protected] ) as the remarkable capture carrier and signal indicator, with the Al 3+ -assisted the fluorescent signal response, we developed a label-free and dual-signal lateral flow immunochromatographic assay (LDLFIA) for sensitive detection of Escherichia coli ( E. coli ) O157:H7. The [email protected] sensing carrier without monoclonal antibodies (mAbs) was manufactured, which adhered to bacteria to form the [email protected] E. coli O157:H7 conjugate, resulting in visible blue band. Then the fluorescent response of the OH-rich [email protected] was excited by introducing Al 3+ , arising from capturing of Al 3+ by -OH through coordination and electrostatic affinity, thus generating a green fluorescent band. Impressively, a smartphone-based portable reading system was developed that can reflect the test results of [email protected] immediately. Under optimum conditions, [email protected] can complete colorimetric and fluorescent mode detection within 90 min, with a detection sensitivity of 10 3 CFU/mL, which were 100 times lower than traditional gold nanoparticles-based LFIA and polymerase chain reaction (PCR). Moreover, the feasibility of the method was further evaluated by the determination of E. coli O157: H7 in drinking water and cabbage with average recoveries of 85.1-123.0%.
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BIOSENSORS & BIOELECTRONICS
·
2017
·
IF 7.78
Qiqi Yuan,Junlin He,Yazhen Niu,Jun Chen,Yilin Zhao,Yuchan Zhang,Chao Yu
α2,3-sialylated glycans (α2,3-sial-Gs) are one of the significant tumour biomarkers for the early diagnosis of cancer. In this work, a neoteric sandwich-type biosensor was developed for detecting α2,3-sial-Gs using 4-mercaptophenylboronic acid (4-MPBA) to construct a novel molecular recognition system by the coordination of a boron atom of 4-MPBA to the amide group of Neu5Ac in the α2,3-sial-Gs structure. Amino-functionalized fullerene coupled with palladium-platinum bimetallic alloy nanocrystals (n-C 60 -PdPt) was synthesized to modify the surface of a glassy carbon electrode (GCE) because the n-C 60 nanomaterial affords a large surface area for the on-site reduction of bimetallic alloy nanoparticles and an excellent capacity for electron transfer . Abundant 4-MPBA were immobilized on the n-C 60 -PdPt, since the 4-MPBA has the mercapto group can combine with PdPt alloy through strong adsorption. Maackia amurensis lectin (MAL) was covalently immobilized on Au-poly (methylene blue) (Au-PMB) acting as the signal amplification components, which was used to recognize the α2, 3-sial-Gs specifically like a second antibody linked on Au-PMB. The differential pulse voltammetry (DPV) current response of the biosensor in 5 mL of PBS (0.1 M, pH = 7.4) was recorded, and the proposed sandwich-type biosensor showed a wide linear range of 10 fg mL −1 −100 ng mL −1 as well as, a low detection limit of 3 fg mL −1 (S/N = 3). Furthermore, the proposed method exhibited good recovery and stability, indicating its potential for use in clinical studies.
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