INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2023
·
IF 8.2
Qizhi Shuai,Yuxiang Liang,Xinrui Xu,Zulala Halbiyat,Xiaowan Wang,Jingwen Cheng,Jialing Liu,Tingjuan Huang,Zhiwei Peng,Lei Wang,Sheng He,Hong Zhao,Zhizhen Liu,Jun Xu,Jun Xie
A thinner endometrium has been linked to implantation failure, and various therapeutic strategies have been attempted to improve endometrial regeneration, including the use of mesenchymal stem cells (MSCs). However, low survival and retention rates of transplanted stem cells are main obstacles to efficient stem cell therapy in thin endometrium. Collagen type III is a key component of the extracellular matrix, plays a crucial role in promoting cell proliferation and differentiation, and has been identified as the major collagen expressed at the implantation site. Herein, composite alginate hydrogel containing recombinant type III collagen (rCo III) and umbilical cord mesenchymal stem cells are developed. rCo III serves as favorable bioactive molecule, displaying that rCo III administration promotes MSCs proliferation, stemness maintenance and migration. Moreover, rCo III administration enhances cell viability and migration of mouse endometrial stromal cells (ESCs). In a mouse model of thin endometrium, the Alg-rCo III hydrogel loaded with MSCs (MSC/Alg-rCo III) significantly induces endometrial regeneration and fertility enhancement in vivo. Further studies demonstrate that the MSC/Alg-rCo III hydrogel promoted endometrial function recovery partly by regulating mesenchymal-epithelial transition of ESCs. Taken together, the combination of Alg-rCo III hydrogel and MSCs has shown promising results in promoting endometrium regeneration and fertility restoration, and may provide new therapeutic options for endometrial disease.
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ACS Applied Materials & Interfaces
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2021
·
IF 9.229
Xiaoyan Yang,Mengjie Zhang,Zixuan Chen,Yuwei Bu,Xue Gao,Yongkun Sui,Yueqin Yu
A simple and selective photoelectrochemical (PEC) biosensor was constructed for Hg2+ detection based on zinc phthalocyanine (ZnPc) dye-sensitized CdS using alginate not only as a carrier but also as a binder. First, CdS as a photoactive material was in situ modified on the electrode surface using a rapid and simple electrodeposition to obtain an initial photocurrent signal. Second, ZnPc was loaded in the amphiphilic alginate micelle and then was coated onto the CdS film surface via alginate as the binder. The photocurrent was subsequently enhanced due to the favorable dye sensitization effect of ZnPc to CdS. Finally, the thymine-rich probe DNA was immobilized on the modified ITO surface via coupling reaction between the carbonyl groups of the amphiphilic polymer and the amino groups of the probe DNA. In the presence of Hg2+, the thymine–Hg2+–thymine (T–Hg2+–T) structure was formed due to the specific bond of Hg2+ with thymine, resulting in the decrease of photocurrent due to the increase of steric hindrance on the modified electrode surface. The proposed PEC biosensor for Hg2+ detection possessed a wide linear range from 10 pM to 1.0 μM with a detection limit of 5.7 pM. This biosensor provides a promising platform for detecting other biomolecules of interest.
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ENVIRONMENTAL RESEARCH
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2020
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IF 5.715
Hongliang Dai,Tongshuai Sun,Ting Han,Zechong Guo,Xingang Wang,Yong Chen
The widespread applications of zinc oxide nanoparticles (ZnO NPs) have raised increasing concerns due to their adverse environmental effects. The ubiquitous natural organic matter in natural aqueous environments can interact with ZnO NPs, thereby affecting their aggregation, sedimentation and biotoxicity. Here, we systematically investigated the effects of humic acid (HA) and sodium alginate (SA) on the aggregation behavior of ZnO NPs and their biotoxicity to Daphnia magna . High concentrations (9.0 mg/L) of HA and SA accelerated the aggregation of ZnO NPs with maximum aggregation rates (ΔD/Δt) of 22.1 and 19.2 nm/min, respectively. Both HA and SA led to 31.2% and 30.1% decrease of ZnO NPs concentration compared with the control experiment. The results calculated by Derjaguin-Landau-Verwey-Overbeek theoretical formula were consistent with these of aggregation and sedimentation of ZnO NPs. Furthermore, excitation-emission-matrix fluorescence spectroscopy verified that the carboxylic groups of HA and SA have high complexation capacity with ZnO NPs. Daphnia magna was used to evaluate the biotoxicity of ZnO NPs, and the toxicity of ZnO NPs to Daphnia magna was alleviated as the HA concentration increased from 0 to 1.2 mg/L. Toxicity mitigation experiments confirmed that photocatalytic generation of reactive oxygen species was more toxic to Daphnia magna than dissolved Zn 2+ in acute and chronic toxicity tests . Moreover, the attacks of active oxygen free radical damaged the antioxidant system of Daphnia magna . The information obtained will help us to improve the understanding of the impacts of ZnO NPs on freshwater ecosystems .
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Journal of Materials Research and Technology-JMR&T
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2020
·
IF 5.289
Ting-Ting Li,Jiaxin Li,Yue Zhang,Jun-Li Huo,Shu Liu,Bing-Chiuan Shiu,Jia-Horng Lin,Ching-Wen Lou
Medical wound dressing with natural antibacterial efficacy is an important issue. However, few studies on Artemisia argyi oil based wound dressing have been studied because of the volatility of the Artemisia argyi oil. In this study, Artemisia argyi oil -microcapsules (AAO-MC)/PVC fibrous membrane were fabricated through emulsification-internal gelation and electrospinning method. The membranes were characterized by SEM, Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), and particle diameter, hydrophilicity, breathability, water vapor transmission rate (WVTR), and antibacterial performance were evaluated. The results show that AAO-MC microcapsules excellent stability, and the slow-release property. Moreover, the prepared AAO-MC/PVC fibrous membrane indicated good WVT (72.47 g/(m²·h)), high hydrophilicity (30.5°), and outstanding antibacterial rate (94.3%). This study provides a new strategy to prepare microcapsule nanofibrous membrane with excellent performance and is suggested to be a promising candidate for medical wound dressings.
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Polymers
·
2020
·Vol. 12
·期 5
·1135
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IF 3.426
Shiying Wang,Yi Zhang,Liupeng Yang,Qizhan Zhu,Qianli Ma,Ruifei Wang,Chaoqun Zhang,Zhixiang Zhang
Traditional pesticide formulations show poor utilization and environmental safety due to their low foliage adhesion and large auxiliaries. In this study, a novel and environment-friendly indoxacarb formulation was prepared to improve the pesticide’s utilization rate, target control characteristics and ecological security. Indoxacarb-loaded waterborne polyurethane–sodium alginate (PU/SA) nanoemulsions with film forming properties, alkaline responsive release, high effectiveness againstSpodoptera litura,and reduced acute contact toxicity for nontarget organisms were successfully prepared. The colloidal properties, swelling and release behaviors, leaf adhesion, degradation dynamics and bioactivity assay of the indoxacarb-loaded PU/SA nanoemulsions were determined. Results showed that the obtained indoxacarb-loaded microcapsule particulates were approximately 57 nm in diameter, electronegative −45.9 mV, and uniformly dispersed in the nanoemulsions. The dried latex films of PU/SA in the alkaline environment revealed better responsive swelling and release characteristics than those in acidic and neutral conditions. Compared with a commercial emulsifiable concentrate, the indoxacarb-loaded PU/SA nanoemulsions were useful for the targeted control ofS. litura, which have alkaline gut and showed reduced acute contact toxicity toHarmonia axyridia. Furthermore, the PU/SA formulation had better foliage adhesion and indicated the property of controlled-release and a persistent effect.Keywords:indoxacarb;anionic polyurethane;sodium alginate;nanoemulsions;bioactivity
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CARBOHYDRATE POLYMERS
·
2020
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IF 7.182
Yihua Yin,Mengdan Yang,Jing Xi,Weiquan Cai,Ying Yi,Guanghua He,Yue Dai,Ting Zhou,Mengqing Jiang
To improve in vitro photostability and enhance insecticidal activity, a novel esterase/glutathione (GSH) responsive photoactivated nano-pesticide delivery system was synthesized by conjugation of photoactivated pesticide phloxine B(PB) to sodium alginate (SA) via esterase/GSH sensitive phenolic ester bond followed by ultrasonic dispersion. The system was stable in PBS (pH 7.4) and could protect effectively the conjugated PB from in vitro photodegradation because of aggregation-caused quenching effect, whose maximum photodegradation rate did not exceed 10 % after 270 min illumination. However, upon exposure to esterase-6 or GSH stimulus, high photoactivity was observed due to the destruction of the system and accompanied by PB release. The combined stimulation could trigger more PB release than any single stimulus and thus resulting in a higher photoactivity. Compared with free PB, The system showed a higher phototoxicity on Sf9 insect cells and the in vitro light exposure had little influence on the phototoxicity.
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SEPARATION AND PURIFICATION TECHNOLOGY
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2021
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IF 7.312
Jing Yu,Yuqi Wang,Yi He,Yixuan Gao,Ruitong Hou,Jing Ma,Liyun Zhang,Xiao Guo,Li Chen
Graphene oxide (GO)-based membranes formed by stacking layered graphene oxide nanosheets have great application prospects for dye separation of textile wastewater. This paper proposes a simple vacuum-assisted method to prepare a double-crosslinked composite graphene oxide nanofiltration membrane (Ca/GO-SAx) by adding sodium alginate (SA) and calcium ions. This method adjusts the nanochannels of the membrane, expands the interlayer spacing, improves the water permeability, and increases the water flux from 10.14 L m -2 h −1 bar −1 to 38.90 L m -2 h −1 bar −1 , which is 384% of the original GO membrane. Importantly, the Ca/GO-SA3 composite membrane has excellent dye separation performance, achieving more than 99% removal of multiple dyes (congo red, crystal violet, methylene blue). Furthermore, the structure of the Ca/GO-SA3 composite membrane is more compact and orderly, and the stability is obviously enhanced. It not only repels other cations in different chloride salt solutions, keeps the interlayer spacing stable, but also maintains excellent dye separation performance after being immersed in harsh aqueous solutions for one month. These findings demonstrate a simple and practical method to prepare GO nanofiltration membranes with a two-dimensional layered structure to achieve the purification treatment of textile wastewater.
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Soft Matter
·
2019
·Vol. 15
·期 11
·2319-2327
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IF 3.399
Na Qi,Hui Zhao,Qiaozhi Wang,Yan Qin,Hao Yuan,Ying Li
Quantum dots (QDs) are of great interest due to their excellent fluorescence properties and thus, they have been widely studied. Compared with the typical organometallic synthetic routes and hydrothermal methods usually carried out under high temperatures, methods employing colloidal templates can be used for preparing QDs in mild conditions and have gained increasing attention. In this prospect, a hydrogel is an ideal colloidal template for the preparation of QDs in an aqueous medium, while the related study for in situ preparation of QDs in a gel and the consequent functionalization of QDs are in demand. In this paper, we proposed a two-step method to prepare CdS QDs in a sodium alginate (SA) gel, which showed effective constraint in the uniform size distribution of QDs. Without the introduction of additional ligands, the prepared CdS–SA QDs exhibited responsiveness to pH and detection of Fe3+, thus providing a simplified way for the functionalization of QDs. CdS–SA QDs showed good biocompatibility and stability in a certain concentration, which indicated the prospective applications of CdS–SA QDs in the fields of biological labeling and environmental sensing.
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ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2019
·Vol. 26
·期 16
·16482-16492
·
IF 2.914
Tong Dongshen,Fang Kai,Yang Haiyan,Wang Jie,Zhou Chunhui,Yu Weihua
Sodium alginate (SA) is a linear biopolymer, which is the nontoxic, biodegradable, and rich in carboxyl and hydroxyl groups. In the paper, the SA-based hydrogel bead was prepared by the cationic hectorite clay and anionic sodium alginate with a simple ionic gelation method under freeze-drying, and the adsorption properties were evaluated by the removal of copper ions from aqueous solutions. The composites were characterized by X-ray diffraction (XRD), nitrogen adsorption-desorption isotherm (BET), thermal analysis (TG), and Fourier transform infrared spectroscopy (FT-IR). The pseudo-first-order and pseudo-second-order kinetic models were used to describe the kinetic data and the Langmuir, Freundlich, Dubinin–Radushkevich (D-R), and Temkin models were applied to describe the adsorption isotherms. The results showed that the adsorption process was found to follow the Freundlich isotherm model and the maximum sorption capacity was observed to be 160.28 mg/g under the initial concentration from 10 to 700 mg/L at 45 °C. Adsorption kinetics data fitted well with pseudo-second-order rate model. The porous structure of the composite was responsible for the adsorption of Cu 2+ ions. But the adsorption ability could be improved by pH. Finally, the adsorption mechanism was suggested.
Graphical abstract
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COMPOSITES PART B-ENGINEERING
·
2019
·
IF 6.864
Rongxiu Ma,Yajing Wang,Houjuan Qi,Cai Shi,Guangbiao Wei,Lidong Xiao,Zhanhua Huang,Shouxin Liu,Haipeng Yu,Chunbo Teng,Hu Liu,Vignesh Murugadoss,Jiaoxia Zhang,Yonggui Wang,Zhanhu Guo
Wound dressings with high flexibility, mechanical strength , and porosity have received increasing attention. Herein, nanocomposite sponges of sodium alginate/graphene oxide (GO)/polyvinyl alcohol were prepared by a freeze-thawing cyclic process and freeze-dried molding. The properties of the sponges were investigated including morphology, mechanical properties , swelling, water vapor permeability, in vitro norfloxacin (NFX) release, antibacterial property , and biocompatibility as well as in vivo wound healing in a mouse model. A GO concentration of 1 wt% resulted in the sponges with a homogeneously porous and interconnected network structure, which exhibited suitable water absorption, breathability , and mechanical properties. Hemolysis tests indicated that the prepared sponges were non-hemolytic material. The CCK-8 assay demonstrated that the presence of an appropriate amount of GO could promote the cell proliferation . The sponges displayed excellent bioavailability of NFX, with a sustained release behavior and a strong inhibitory effect against both Escherichia coli and Staphylococcus aureus . The in vivo evaluations demonstrated that the sponges enhanced wound healing. Therefore, these novel nanocomposite sponges are promising candidates for wound healing applications.
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FOOD HYDROCOLLOIDS
·
2019
·
IF 5.839
Xiuqin Fang,Xinyu Zhao,Gaobo Yu,Lei Zhang,Yuhong Feng,Yang Zhou,Yuanyuan Liu,Jiacheng Li
Biopolymer-based Pickering emulsions have attracted increasing interest in research areas including food , pharmaceutics and drug delivery. However, understanding of self-assembly behavior from the dedicate structure of stabilizers has not been well explored yet. In this work, we proposed to investigate how molecular weight affect the self-assembly behavior and to explore the potential of biopolymer colloidal particle-stabilized Pickering emulsion for controlling release of hydrophobic drugs. Amphiphilic sodium alginate derivatives (Ugi-Alg) with three molecular weights (685 kDa, 307 kDa and 48 kDa) were successfully incorporated with a hydrophobic group. Based on the characterizations of morphology and rheology behavior, the high-molecular-weight derivatives (H-Ugi-Alg) can form a compact micelle structure in aqueous solution. Emulsions stabilized by H-Ugi-Alg exhibited a higher stability and stronger viscoelasticity than those using Ugi-Alg with a lower molecular weight. In addition to the entangled polymer chains caused by higher molecular weight and hydrophobic association, the partially protonated carboxyl groups reduced electrostatic repulsion at pH = 4.2 also facilitate the formation of compact micelle structure. Approximately 78.6% of the encapsulated curcumin released from the emulsion stabilized by H-Ugi-Alg, which exhibits a similar release efficiency compared with the nanoparticle-stabilized Pickering emulsions.
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RSC Advances
·
2018
·Vol. 8
·期 73
·42123-42132
·
IF 2.936
Zhongxiang Bai,Weihua Dan,Guofei Yu,Yanjun Wang,Yining Chen,Yanping Huang,Changkai Yang,Nianhua Dan
Natural collagen has good biocompatibility and ability to promote tissue regeneration; however, its low flexibility and easy degradation hinder its applications in wound repair. In this study, we synthesized a skin wound-repairing hydrogel with good bioactivity and high toughness and adhesion. Inspired by the good adhesion of natural mussels, dopamine was grafted onto oxidized sodium alginate to synthesize a new crosslinker (COA), which was introduced into the collagen/polyacrylamide (PAM-Col) double network to synthesize hydrogel. The morphological characterization of the hydrogel using scanning electron microscopy confirmed that the hydrogel formed a more chaotic interconnected structure after the introduction of COA. PAM-Col-COA hydrogel had good mechanical properties, skin tissue adhesion, water absorption, and sustained biological activity. In vivo wound healing experiments showed that hydrogel accelerates the wound healing process and has potential applications in wound dressings.
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