Frontiers in Nutrition
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2025
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IF 5.1
Siyu Dai,Cunjie Li,Dagang Li,Hong Wang
Introduction: This study investigates the effects of α-GML on intestinal epithelial tight junction (TJ) protein expression and its molecular mechanisms. Recognizing the critical role of TJ proteins in intestinal barrier function and the potential of α-GML to enhance this barrier, we employ the IPEC-J2 cell model. Our aim is to validate the regulatory impact ofα-GML on TJ protein expression and elucidate the underlying signaling pathways, thereby offering new strategies for intestinal health maintenance.Methods: Utilized Data-Independent Acquisition (DIA) analysis to identify optimal targets of α-GML in modulating Tight Junction (TJ) protein expression. Treated cells with specific inhibitors of PKC and MAPK to assess their role in TJ regulation by α-GML. Co-treated cells with the MAPK inhibitor SCH772984 and α-GML to study the effects on p-ATF-2 expression. Evaluated the effects of SCH772984 and ATF-2 overexpression on the protein expression levels of phosphorylated ATF-2, ZO-1, and OCLN.Results: Revealed that α-GML’s modulation of TJ proteins might involve the PKC/MAPK signaling pathway, leading to ATF-2 phosphorylation. Both PKC and MAPK inhibitors reduced TJ protein expression (p < 0.05, p < 0.01 or p < 0.001), indicating their involvement in α-GML’s regulation. SCH772984 counteracted α-GML-induced upregulation of p-ATF-2 (p < 0.05), suggesting MAPK’s role in this process. Identified potential ATF-2 binding sites on ZO-1 and OCLN promoters. ATF-2 significantly enhanced ZO-1 promoter activity (p < 0.001). SCH772984 reduced phosphorylated ATF-2, ZO-1, and OCLN levels (p < 0.05 or p < 0.01), while ATF-2 overexpression rescued this decrease (p < 0.05 or p < 0.01), confirming ATF-2’s role in TJ protein upregulation via the MAPK pathway.Discussion: Our study indicated that α-GML enhanced the expression of TJ proteins through the PKC/MAPK/ATF-2 pathway, thereby enhancing the barrier function of intestinal epithelial cells.
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Journal of Asian Ceramic Societies
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2025
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IF 2.1
Maohui Li,Mingjun Zhang,Bo Ma,Wenzhou Sun,Yanmin Wang,And Youjun Lu
While graphene is widely acknowledged for its potential in enhancing the toughness of ceramic composites, achieving substantial mechanical improvements through simple and scalable processing methods remains a challenge. This study demonstrates that minimal additions of graphene nanoplatelets (GNPs) can significantly enhance the fracture toughness of silicon carbide (SiC) ceramics using pressureless sintering. The investigation focused on examining the influence of GNPs addition on the phase composition, porosity, mechanical properties, and microstructure of these composite ceramic materials. By incorporating only 1–3 wt.% GNPs into an α-SiC matrix, a remarkable 37.86% increase in fracture toughness (5.28 MPa·m1/2) is achieved at the optimal loading of 1 wt.%, surpassing the toughening efficiency typically reported for similar composite systems. Microstructural analysis revealed that the primary toughening mechanisms of GNPs in SiC involve pull-out and crack deflection. This study demonstrates GNPs combined with pressureless sintering can effectively unlock the toughness potential of SiC ceramics, providing a practical and scalable approach for industrial applications.
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CONSTRUCTION AND BUILDING MATERIALS
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2025
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IF 8
Ting Xiao,Siyu Chen,Yu Zheng,Lihua Jiang,Xinyi Li,Yulong Qiao,Xinyu Tan
Addressing the high energy consumption and greenhouse gas emissions associated with conventional active cooling technologies, daytime passive radiative cooling has attracted significant interest due to its zero-energy cooling characteristics. However, its practical application remains challenging due to the difficulty in balancing optical performance, fabrication cost, process complexity, and environmental durability within existing material systems. This study utilizes industrially derived α-hemihydrate phosphogypsum (α-HPG) as a functional inorganic filler to develop an SPG-DM 0.6 S composite coating with integrated cooling performance, superhydrophobicity, and self-healing capability through hydrophobic group grafting and compounding with polymethyl methacrylate (PMMA). Experimental results demonstrate that the coating achieves an average temperature reduction of 19.5°C below ambient temperature, a net cooling power of 128 W·m −2 , a WCA of 161.2°, and excellent anti-icing and self-cleaning performance. Finite-Difference Time-Domain (FDTD) simulations systematically reveal the regulation mechanism of the material's microstructure on light scattering efficiency. This work pioneers a novel approach for the high-value utilization of industrial by-products and provides an innovative solution for developing cost-effective, high-performance, and durable sustainable radiative cooling materials.
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AICHE JOURNAL
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2025
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IF 4
Xueqi Shi,Kai Liu,Na Wang,Qiuyan Ding,Zhenyu Zhao,Hong Li,Xin Gao
The actual applications of extraction methods for α -olefins separation are limited by their long time and high energy consumption. Hence, this study employs microwave technology to intensify the extraction process using Ag-based ionic liquid (Ag-IL), where the influence of microwave irradiation and the mechanism of microwave-assisted ionic liquid extraction (MAILE) are investigated, along with a comprehensive techno-economic analysis of process feasibility. The results of COSMO-RS and DFT simulations demonstrate the dominant role of π-complexation in identifying Ag-IL with high selectivity, indicating the possible enhancement caused by microwave irradiation. The subsequent kinetic experiments show that compared with conventional routes, the MAILE accelerated the extraction process by approximately 89.72%. As a result, the total annual cost of unit product of the overall process is reduced by 6.43% according to techno-economic analysis by Aspen Plus, indicating that MAILE is a cost-effective, scalable, and sustainable approach to α -olefin separation, offering process efficiency and environmental sustainability.
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FOOD CHEMISTRY
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2018
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IF 4.946
Xiao-dan Shi,Ou-ye Li,Jun-yi Yin,Shao-ping Nie
Dictyophora echinovolvata is a kind of edible mushroom in the Dictyophora genus, of which polysaccharide is an important chemical substance. Herein, three polysaccharide fractions (DEP-4P, DEP-6P and DEP-8P) were prepared from water extract of D. echinovolvata using gradient ethanol precipitation method. Their chemical structures were analyzed by methylation analysis and 1D/2D NMR spectroscopy. Molecular weights were determined by multi-angle laser light scattering combined with size-exclusion chromatography (HPSEC-MALLS). HPSEC data showed that DEP-4P had the highest values of molecular weight, intrinsic viscosity and hydrodynamic radius. DEP-6P and DEP-8P had lower molecular weights, which contributed to their easily distinguished 1D and 2D NMR spectra. Methylation and NMR analysis suggested that the three fractions were linear α-(1 → 4)-glucans with α-Glc p residues linked to the backbone at C -6. Differences among the three fractions were the molar ratios of the identified glycosidic bonds. Repeating units of polysaccharides from D. echinovolvata were proposed as follows: Download : Download high-res image (60KB) Download : Download full-size image
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BMC Complementary Medicine and Therapies
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2022
·Vol. 22
·期 1
·1-13
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IF 2.838
Fu Weiyu,Liao Xuehua,Zhang Qian,Zhu Yuzhen,Mei Si,Li Qian,Zhou Xin,Li Xiaojun,Luo Hui,Ye Hua,Wu Kefeng
Background Polysaccharides from wampee have been reported to process various biological activities, while the relationship between structure and bioactivities has been barely addressed. Pectin, an abundant water-soluble polysaccharide in wampee, showed significant antioxidant activity, which was associated with the anti-melanogenic activity. Therefore, this study investigated the physicochemical characteristics and the anti-melanogenesis effect of pectin extracted from wampee fruit in A375 cells.Methods The physicochemical characterization of pectin from wampee fruit was investigated by gel chromatography (GCP), FT-IR spectroscopy, and NMR spectroscopy methods. The anti-melanogenesis effects and mechanism were evaluated by mushroom tyrosine enzyme and human melanin cell model in vitro.Results The results showed that a molecular weight of 5.271 × 105 Da wampee fruit pectin (WFP) were mainly composed of mannose (Man), ribose (Rib), rhamnose (Rha), glucuronic acid (Glc A), glucose (Glc), galacturonic acid (Gal A), galactose (Gal), and arabinose (Ara), which linked with →4)-β-D-Galp-(1 → units. The current study revealed that WFP could significantly suppress mushroom TRY activity in vitro. Furtherly, WFP significantly reduced intracellular and extracellular melanin formation in A375 melanoma cells depending on the presence of alpha-melanocyte stimulating hormone (α-MSH). TRY activity was only inhibited in α-MSH treated A375 cells. Western blot analysis demonstrated that WFP reverse α-MSH induced melanogenesis in A375 melanoma cells, including in down-regulated TRY, TYRP-1, TYRP-2, MITF and CREB expressions.Conclusion These results indicated that WFP could inhibit α-MSH induced melanogenesis in A375 melanoma cells via α-MSH/TRY pathway. In conclusion, these data provided a new perspective to annotate WFP anti-melanogenesis activity mechanism.
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LIFE SCIENCES
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2020
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IF 3.647
Lixia Bu,Xiaohong Cao,Zilong Zhang,Huiwen Wu,Renwei Guo,Mingfeng Ma
Aims Macrophages, as an important member of immune system, engulf and digest pathogens in innate immunity and help initiate adaptive immunity. However, macrophages also involve in occurrence and development of many diseases, such as obesity and type 2 diabetes. Here, we aimed to reveal how activated macrophages cause insulin resistance in skeletal muscle in vitro through simulating body environment. Main methods We established RAW264.7 macrophages and C2C12 myotubes co-incubation model in vitro using Transwell filter to simulate body environment and investigated effects of RAW264.7 cells on insulin-regulated glucose metabolism in C2C12 myotubes. Immunofluorescence, Immunoblot and glucose uptake tests were used to assess metabolic changes in C2C12 myotubes. ELISA test detected secretions of tumor necrosis factor α (TNF-α) and interleukin-6 (IL-6) from RAW264.7 cells. In addition, RNA interference and inhibitor treatment were used. Key findings Activated RAW264.7 cells attenuated insulin response in C2C12 myotubes. Activated RAW264.7 cells secreted a lot of TNF-α and IL-6. We found that TNFα, but not IL-6, caused insulin resistance of skeletal muscle in a dose-dependent manner. The results further indicated that activation of TNF-α downstream proteins, inhibitor of nuclear factor κ-B kinase (IKK) and the jun-N-terminal kinase 1 (JNK1) led to phosphorylation of insulin receptor substrate 1 (IRS-1) at Ser residues and insulin resistance in C2C12 myotubes. Significance Our research provided further and direct demonstration on activated macrophage-induced insulin resistance in skeletal muscle, suggesting TNF-α might become a therapeutic target to ameliorate and treat type 2 diabetes.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2018
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IF 3.909
Taotao Dai,Jun Chen,Qian Li,Panying Li,Peng Hu,Chengmei Liu,Ti Li
Procyanidins were reported to have an inhibitory effect on α-amylase, but the interaction mechanism between procyanidins and α-amylase was rarely reported. Spectroscopic and molecular docking techniques were utilized to explore the interaction between porcine pancreatic α-amylase (PPA) and B-type procyanidin dimer (PB2). PB2 decreased the intrinsic fluorescence and surface hydrophobicity of PPA, indicating that an interaction occurred and complex formed. The binding process of complex was spontaneous and the main interaction was hydrophobic force. Circular dichroism showed conformational changes of PPA with an increasing of α-helix and β-sheet structure. Molecular docking speculated that PB2 could form hydrophobic force with PPA by bind to the active sit (Asp 167, Asn 100, Arg 158, His 201). This research can offer new insights into the mechanism of PB2 in inhibiting PPA catalysis and provide useful information on dietary recommendation of PB2 for the treatment of type 2 diabetes.
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Food & Function
·
2021
·Vol. 13
·期 2
·857-866
·
IF 5.396
Ming He,Yuhan Zhai,Yuqing Zhang,Shuo Xu,Shaoxuan Yu,Yingxin Wei,Haifang Xiao,Yuanda Song
α-Glucosidase is related to the increase in postprandial blood glucose in vivo. Inhibition of α-glucosidase is supposed to be an effective approach to treat type 2 diabetes mellitus (T2DM). Trilobatin, a member of the dihydrochalcone family, shows anti-oxidant, anti-inflammatory and anti-diabetic activities. In this study, the inhibitory activity and mechanism of trilobatin on α-glucosidase were investigated using multispectroscopic and molecular docking techniques. The kinetic analysis showed that trilobatin reversibly inhibited α-glucosidase in a noncompetitive-type manner and the value of IC50 was 0.24 ± 0.02 mM. The analysis of fluorescence spectra demonstrated that the formation of the trilobatin-α-glucosidase complex was driven mainly by hydrogen bonding and van der Waals forces, resulting in the conformational changes of α-glucosidase. Fourier transform infrared spectroscopy (FT–IR) and circular dichroism (CD) measurements suggested that the interaction could change the micro-environment and conformation of α-glucosidase affected by trilobatin. Molecular docking analysis determined the exact binding sites of trilobatin on α-glucosidase. These results indicated that trilobatin is a strong α-glucosidase inhibitor, thus it could be conducive to ameliorate T2DM.
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JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
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2020
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IF 3.004
Xiaorong Hou,Xiaoyi Lou,Qian Guo,Lan Tang,Weiguang Shan
Immobilized liposome chromatography (ILC) is a powerful tool in screening and analyzing membrane-permeable components in natural medicinal herbs. In this study, the stationary phase of a new receptor liposome biomembrane chromatography (RLBC) was employed to screen the active ingredients in seven natural medicinal herbs. As a model system, α-glucosidase was immobilized in porous silica gel by incorporating α-glucosidase into liposome vesicles. Combined with HPLC, this stationary phase can be used to evaluate the interaction of liposome-protein compounds with compounds and drugs. The surface characteristics of the RLBC phase was characterized by Scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FTIR) and Energy dispersive spectrometer (EDS). RLBC was successfully used to screen and analyze permeable compounds in natural medicinal herbs, and screen the extracts from Schisandra chinensis. This method was used to establish that Schisandrin in Schisandra chinensis is an active component. Furthermore, the hypoglycemic effect of Schisandrin was verified in vivo in rats. This study further modeled the relationship between the activity of inhibitor and retention behavior with RLBC with a mathematical equation. RLBC stationary phase combined with HPLC can be used for rapid screening of drug candidates.
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MOLECULES
·
2024
·Vol. 29
·期 23
·5622
·
IF 4.2
Shengxue Zhang,Wangchuan Xiao,Jingjing Wu,Fanhong Wu,Houjin Huang,Xiaoyu Ma,Yafei Shi,Chao Liu
A novel and efficient 1,2-oxidative trifluoromethylation of olefins employing Ag(O 2 CCF 2 SO 2 F) as a trifluoromethyl source is described with O 2 as the oxidant, which provides access to a variety of valuable α-trifluoromethyl-substituted ketones. The broad substrate scope, feasibility of large-scale operation, and derivatization reactions of α-trifluoromethyl ketones demonstrate the promising utility of this protocol.
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JOURNAL OF LUMINESCENCE
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2024
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IF 3.3
Haoyu Ma,Xiaoling Niu,Wenlong Qu,Yongliang Liu,Zhen Feng,Shengbo Zhu
Aggregation induced emission luminogens (AIEgens) as one of the optimal alternatives for designing stimuli-responsive fluorophores for real-life applications have sparked much current interest in smart materials design. Herein, the “Star molecules” in AIEgens of α-cyanostilbene and Tetraphenylethylene (TPE) derivatives were constructed into one molecular, namely DC-TPE-tBu and DC-TPE-CN. Both compounds exhibited significant AIE properties. Moreover, the two compounds could change their luminescent colors and intensities under the stimulation of external mechanical force and UV light, showing excellent mechanochromic luminescent (MCL) and remarkable photo-responsive behaviors, respectively. The stimuli responsive mechanisms were investigated by powder X-Ray diffractometer (PXRD) spectra and density functional theory calculations (DFT). The MLC behaviors were associated with the crystalline-to-amorphous transitions after mechanical grinding. These remarkable features make them suitable for the application of mechano/photo-sensors. The studies provide a useful strategy for developing AIE-active functional materials.
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