BIOCHEMICAL ENGINEERING JOURNAL
·
2016
·
IF 2.463
Xiangmin Miao,Zhiyuan Cheng,Zongbing Li,Po Wang
This work described a label-free platform for sensitive cholesterol detection upon the precipitation of cetyltrimethyl ammonium bromide (CTAB) stabilized gold nanoparticles (AuNPs). Modifying with CTAB, the stability of AuNPs was greatly improved compared to conventional used AuNPs, and the surface of them was positively charged, which could effectively adsorb with negatively charged DNA detection probe (DP) based on electrostatic adsorption, and inducing the precipitation of them. Tremendous H 2 O 2 would produce based on the reaction of cholesterol with oxygen by using cholesterol oxidase (ChO x ) as the catalyzer, and followed the happen of Fenton reaction in the presence of Fe 2+ to generate OH , inducing the cleavage of DP into DNA fragments. As a result, the adsorption ability of AuNPs decreased, accompanying with a decrease of AuNPs precipitation. Then, based on detecting the absorption spectra change of AuNPs in supernatant along with the increase of cholesterol concentration, sensitive and simple visual-based cholesterol detection was realized with a detection limit of 335 pM. Such method showed high selectivity for the detection of cholesterol. Moreover, the high stability of such AuNPs in solution that contained salt, protein or other metal ions, making our sensing method have a potential application in bioanalysis.
✓ 已验证
产品关联
DOI
高影响因子
ELECTROCHIMICA ACTA
·
2016
·
IF 4.803
Guangran Ma,Min Yang,Chenyi Li,Haiyan Tan,Liang Deng,Shi Xie,Fugang Xu,Li Wang,Yonghai Song
Nickel-cobalt oxide nanowrinkles with spinel-type crystal structure supported on reduced graphene oxide (NiCo 2 O 4 NWs-rGO) was prepared to develop a sensitive and stable nonenzymatic glucose sensor. The NiCo 2 O 4 NWs-rGO hybrid were prepared by a facile one-pot hydrothermal reaction, and sequential calcination in air. The morphology, composition and crystal structure of the NiCo 2 O 4 NWs-rGO hybrid were characterized by scanning electron microscope, transmission electron microscope, selected area electron diffraction, and energy-dispersive spectroscopy. The electrochemical behavior of the hybrid and its catalytic activity towards glucose oxidation were investigated by several electrochemical methods. Compared with single component NiO or Co 3 O 4 , spinel type NiCo 2 O 4 NWs displayed higher catalysis towards glucose oxidation. Further integration of NiCo 2 O 4 with graphene could reduce the overpotential and enhance the catalytic current due to the improved conductivity and dispersity of NiCo 2 O 4 . The NiCo 2 O 4 NWs-rGO based glucose sensor showed a wide linear range of 0.005-8.6 mM, a low detection limit of 2 μM (S/N = 3), and an improved stability. A satisfactory recovery was also obtained for glucose detection in human serum at physiological level. Our results indicate rationally combine spinel type mixed metal oxide with graphene is a good alternative to fabricate advanced metal oxide based electrochemical sensors.
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2024
·
IF 8.3
Zehua Hou,Tianjun Zhou,Liangjiu Bai,Wenxiang Wang,Hou Chen,Lixia Yang,Huawei Yang,Donglei Wei
Hydrogels, as flexible materials, have been widely used in the field of flexible sensors. Human sweat contains a variety of biomarkers that can reflect the physiological state of the human body. Therefore, it is of great practical significance and application value to realize the detection of sweat composition and combine it with human motion sensing through a hydrogel. Based on mussel-inspired chemistry, polydopamine (PDA) and gold nanoparticles (AuNPs) were coated on the surface of cellulose nanocrystals (CNCs) to obtain CNC-based nanocomposites (CNCs@PDA-Au), which could simultaneously enhance the mechanical, electrochemical, and self-healing properties of hydrogels. The CNCs@PDA-Au was composited with poly(vinyl alcohol) (PVA) hydrogel to obtain the nanocomposite hydrogel (PVA/CNCs@PDA-Au) by freeze–thaw cycles. The PVA/CNCs@PDA-Au has excellent mechanical strength (7.2 MPa) and self-healing properties (88.3%). The motion sensors designed with PVA/CNCs@PDA-Au exhibited a fast response time (122.9 ms), wide strain sensing range (0–600.0%), excellent stability, and fatigue resistance. With the unique electrochemical redox properties of uric acid, the designed hydrogel sensor successfully realized the detection of uric acid in sweat with a wide detection range (1.0–100.0 μmol/L) and low detection limit (0.42 μmol/L). In this study, the dual detection of human motion and uric acid in sweat was successfully realized by the designed PVA/CNCs@PDA-Au nanocomposite hydrogel.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Xinyi Wang,Jing Wang,Houcheng Ding,Yongping Dong
Covalent organic framework (COF) materials exhibit potential application in electrochemical sensing and attract increasing research interests. In this study, a novel signal-off mode electrogenerated chemiluminescence (ECL) sensor based on gold nanoparticle decorated COF (Au@COF) composite was developed. The oxidation of luminol was promoted at ferrocene (Fc) modified electrode to generate strong anodic ECL signal. The resonance energy transfer (RET) between luminol ECL and Au@COF composite could inhibit ECL emission. The acetylcholinesterase (AChE) aptamer connected Au@COF composite was immobilized onto the electrode through DNA hybridization to construct ECL-RET system. The specific recognition between AChE and its aptamer could release Au@COF composite from the electrode surface to block the ECL-RET process and restore the ECL signal. As a result, an ‘‘off-on’’ type ECL sensor was constructed and used to detect AChE in the range of 0.5 nM-1 μM, with a detection limit of 1.7 × 10 -10 mol/L (3σ). The sensor has high sensitivity and good stability, indicating that COF composite has attractive potential application in ECL sensing field.
✓ 已验证
产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 5
·1-9
·
IF 5.3
Yan Qinghua,Wu Ruixue,Wang Jiaxin,Mao Jian,Nan Wenbin
Abstract To accurately detect tumor marker carbohydrate antigen 72-4 (CA72-4) of serum samples is of great significance for the early diagnosis of malignant tumors. In the present study, MnO 2 /hollow nanobox metal-organic framework (HNM)-AuPtPd nanocomposites were prepared via multi-step synthesis and superposition method and a series of characterizations were carried out. A highly sensitive immunosensor Ab/MnO 2 /HNM-AuPtPd/GCE based on the composite nanomaterial was further prepared and used to detect the tumor marker CA72-4. The constructed immunosensor achieved signal amplification by increasing the electrocatalytic activity to H 2 O 2 by means of the synergistic effect of MnO 2 ultra-thin nanosheets (MnO 2 UNs) and HNM-AuPtPd. At the same time, the electrochemical properties of the immunosensor were analyzed using cyclic voltammetry, electrochemical impedance, amperometry (with the test voltage of −0.4 V), and differential pulse voltammetry. The experimental results showed that the MnO 2 /HNM-AuPtPd nanocomposites were successfully prepared, and the immunosensor Ab/MnO 2 /HNM-AuPtPd/GCE demonstrated an excellent electrochemical performance. The electrochemical immunosensor had the highest detection sensitivity under the optimal experimental conditions, such as incubation pH of 7.0, incubation time of 60 min, with the addition of 15 μL of H 2 O 2 , and in the concentration range 0.001–500 U/mL. It had a low detection limit of 1.78×10 −5 U/mL (S/N = 3). Moreover, the serum sample recovery were in the range from 99.38 to 100.52%. This study provides a new method and experimental basis for the detection of tumor markers in clinical practice. Graphical Abstract
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2024
·
IF 6.7
Yun-Dong Yin,Lei Yang,Xi-Tong Song,Jun Hu,Fang-Fang Chen,Ming Xu,Zhi-Yuan Gu
The high expression of Spermidine/spermine N1-acetyltransferase (SSAT-1) is an important indicator in early cancer diagnosis. Here, we developed a nanopore-based methodology with γ-cyclodextrin as an adaptor to detect and quantify acetylamantadine, the specific SSAT-1-catalyzed product from amantadine, to accordingly reflect the activity of SSAT-1. We employ γ-cyclodextrin and report that amantadine cannot cause any secondary signals in γ-cyclodextrin-assisted α-HL nanopore, while its acetylation product, acetylamantadine, does. This allows γ-cyclodextrin to practically detect acetylamantadine in the interference of excessive amantadine, superior to the previously reported β-cyclodextrin. The quantification of acetylamantadine was not interfered with even a 50-fold amantadine and displayed no interference in artificial urine sample analysis, which indicates the good feasibility of this nanopore-based methodology in painless cancer prediagnosis. In addition, the discrimination mechanism is also explored by 2-D nuclear magnetic resonance (NMR) and nanopore experiments with a series of adamantane derivatives with different hydrophilic and hydrophobic groups. We found that both the hydrophobic region matching effect and hydrophilic interactions play a synergistic effect in forming a host–guest complex to further generate the characteristic signals, which may provide insights for the subsequent design and study of drug-cyclodextrin complexes.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2024
·
IF 4.9
Linghui Cao,Yuanxinyi Zhou,Tianyu Shen,Aijuan Xie,Shiping Luo,Baozhu Yang
Dopamine (DA), widely recognized as the most prevalent catecholamine neurotransmitter in the brain, functions as a vital regulator of various physiological processes within the central nervous system. Meanwhile, uric acid (UA) serves as the terminal product of purine metabolism, and hyperuricemia emerges as a significant risk factor for gout, with a steep escalation in risk as blood uric acid levels surge. Consequently, monitoring the concentrations of DA and UA in biological fluids is crucial not only for the treatment of related disorders but also for enhancing disease prevention strategies. In this work, the blooming hydrangea shaped ZnCo(OH)F/carbon dots (CDs) composite was grown on carbon cloth (CC) substrate by a simple hydrothermal method, which was used as a flexible sensor for the detection of DA and UA. The unique open structure and morphologic adjustment strategy of ZnCo(OH)F/CDs/CC provided a large surface area and highly exposed active site. After screening the experimental variables that affect the sensor performance, the optimal ZnCo(OH)F/CDs/CC flexible electrode achieved individual and simultaneous detection for DA and UA in the concentration ranges of 1.0 220.0 µM and 1.0 500.0 µM, the detection limits (LOD) of DA and UA were 0.0129 and 0.044 μM, respectively (S/N=3). In addition, the sensor exhibited good repeatability, stability and selectivity, realizing the detection of DA and UA in human serum with recovery rate of 95.20 % 105.3 %. Also, the density functional theory (DFT) calculations were used to explore the interaction between electrode material and detected substrate. A simple hydrothermal method was utilized to grow the ZnCo(OH)F/CDs composite onto the CC substrate. The presence of F − in ZnCo(OH)F can increase the charge mobility and improve the conductivity. ZnCo(OH)F/CDs/CC as flexible electrode material can determine DA and UA simultaneously in human serum with a satisfactory recovery rate. This flexible sensor has a broad application prospect in the manufacture of wearable devices.
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Jing Li,Jijie Shi,Hui Ren,Junjie Yang,Dan Xiao,Xiaoqin Li,Xinhui Wang,Yong Guo,Zhaoyu Jin
A novel non-enzymatic glucose sensor is developed by directly growing flower-shaped NiCo 2 O 4 microsphere on flexible carbon cloth (NCO/CC) through a one-step electro-deposition with subsequent mild heat treatment. Benefiting from the excellent conductivity between the three-dimensional (3D) structure of NiCo 2 O 4 and CC, as well as the synergic effect between the nickel and cobalt cations, the NCO/CC can not only offer more additional active sites, but also significantly enhance its electrocatalytic performance. Under the optimized conditions, the NCO/CC electrode reveals excellent activity with a broad linear detection range from 10 to 2000 μM, a high sensitivity of 5260 μA mM −1 cm −2 , and a low detection limit (LOD) of 0.2 μM, along with good repeatability and long-term stability. Furthermore, the NCO/CC was successfully utilized to detect glucose in actual beverage samples, showing competitive performance. The straightforward and practical method offers a novel approach to measuring glucose in real-world applications. Novelty statement 3D flower-shaped NiCo 2 O 4 microspheres growing on flexible carbon cloth (NCO/CC) were fabricated by a facile one-step electro-deposition with subsequent mild heat treatment. The fabricated NCO/CC electrode exhibits a wide linear detection range, high selectivity, good reproducibility, and stability towards the detection of glucose.
✓ 已验证
产品关联
DOI
高影响因子
Scientific Reports
·
2024
·Vol. 14
·期 1
·1-14
·
IF 3.8
Bian Lijun,Su Xinglin,Wang Jialu
Carbon cloth (CC) was electrochemically activated using three electrochemical methods in different electrolytes. The gases released during the activation process etched the surface of the CC, thereby increasing its surface area of the activated CC (FCC). Moreover, functional groups such as oxygen-containing groups and N-doping have been introduced. The quantity and type of functional groups introduced during the activation process were related to the anions in the solution. FCC electrodes were used to construct electrochemical sensors for the simultaneous determination of ascorbic acid (AA), dopamine (DA), and uric acid (UA). The PFCC electrode activated by advanced cyclic voltammetry in (NH 4 ) 2 HPO 4 showed linear ranges for AA, DA, and UA concentrations of 0.1 to 2.1 mM, 0.5 to 11 µM, and 0.5 to 11 µM, respectively. The detection limits are 72.93, 0.22, and 0.42 µM, respectively. The good flexibility the PFCC electrode made it suitable for the preparation of flexible sensors. The simultaneous determination of AA, DA, and UA in fetal bovine serum showed a reliable recovery ratio. This study provides a simple and green approach for activating carbon cloth and constructing flexible electrochemical sensors with the potential to simultaneously detect AA, DA, and UA.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Nano Materials
·
2024
·
IF 5.3
Tingting Li,Yuzhuo Tuo,Yunqi Hao,Jingyu Pang,Miaomiao Tian,Samuel Lalthazuala Rokhum,Fang Zhang,Fang Chai
Determination of Hg2+ is crucial as its high toxicity poses a potential serious threat to food and the environment. Therefore, it is urgent to explore a portable, cost-effective, and visual approach to monitor Hg2+. Herein, ratiometric-constructed dual-emissive carbon dots (P-R-CDs) with dual emissions at 440 and 575 nm were fabricated by blue CDs and orange CDs for visual quantitative detection of Hg2+. The vivid colorimetric sensing can be achieved by quantitative quenching blue emission with Hg2+ and relative stable orange emission. Consecutive fluorescent color transformation with elimination of blue emission by Hg2+ embodied visible distinguishable determination of Hg2+, with a detection limit of 2.9 nM. Furthermore, a smartphone-supported visual sensing platform was established for the determination of Hg2+ by capturing and identifying the RGB value of the variation precisely. The smartphone assistant detection greatly promoted the effectiveness of detection and provided promising on-site application in real samples. The recoveries in real water and food samples revealed the excellent environmental tolerance of P-R-CDs, which represented a prospective application utility for quantitative monitoring of Hg2+ in food and the environment.
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2025
·
IF 6.1
Jinhang Guo,Hong Han,Hong Zhao,Deqi Jia,Ling Yin,Jingquan Sha
Nanozymes with biological enzyme activity show great promise in biochemical analysis and medicine, yet single-activity nanozymes were limited by low catalytic efficiency and strict catalytic environment requirements. Consequently, the development of nanozymes with multiple enzyme activities presents a significant challenge. In the work, a vanadium-capped polyoxometalates (POMs)-encapsulating metal-organic framework (MOF), [Cu 12 (Trz) 8 Cl][PMo 12 O 40 (VO) 2 ], was synthesized via hydrothermal synthesis, which shows multiple enzyme activities concluding oxidase (OXD), peroxidase (POD) and catalase (CAT) activities. In the catalytic procedure, a fraction of H 2 O 2 generated by the OXD is further subjected to the POD catalytic reaction and the remaining portion is transformed into O 2 through the CAT activity, in turn, supplies the driving force for the OXD-like catalytic process. This cascade reaction, in conjunction with the transformation between Cu 2+ and Cu + within the material, engenders a structure analogous to an interlocking "gear", which augments the catalytic efficacy as well as the adaptability to intricate environmental conditions of the [Cu 12 (Trz) 8 Cl][PMo 12 O 40 (VO) 2 ] enzyme. By capitalizing on this high catalytic efficiency, the rapid quantitative detection of glutathione (GSH) was established with the calculated limit of detection (LOD) 0.062 μM in the range of 5–60 μM. Surprisingly, the multi-enzymatic [Cu 12 (Trz) 8 Cl][PMo 12 O 40 (VO) 2 ] can concurrently enhance the generation of reactive oxygen species (ROS) and the depletion of GSH in the tumor microenvironment (TME), thereby inducing tumor cell apoptosis. This research holds promising application prospects in the fields of biotechnology, clinical diagnosis, and tumor therapy.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Chemical Science
·
2025
·
IF 7.4
Baoting Dou,Hui Shen,Zhimin Li,Huanyu Cheng,Po Wang
Reactive oxygen species (ROS) play a critical role in regulating various physiological processes. To gain a comprehensive understanding of their distinct functions in different physiological events, it is imperative to detect binary ROS simultaneously. However, the development of the sensing method capable of binary ROS detection remains a significant challenge. In this study, we address this challenge by integrating chemically modified DNAzyme probes with the functionalized metal-organic framework (MOF) to create an efficient electrochemical sensing platform for the binary detection of ROS. ROS targets would activate the DNAzyme cleavage activity by removing the phenylboronate (BO) and phosphorothioate (PS) modifications, leading to the controlled release of doxorubicin (DOX) and methylene blue (MB) enclosed within MOF nanocomposites. This process generates two distinct voltammetric current peaks, with their potentials and intensities reflecting the identity and concentration of the ROS targets. The sensor demonstrates simultaneous detection of multiple ROS (H2O2 and HClO) produced by cancer cells with high sensitivity across a broad linear range of 1 to 200 nM and a low detection limit of sub-nanomolar. The design strategies behind the developed ROS sensing system could also be exploited to create other biosensors with highly sensitive and binary detection to promote clinical research and revolutionize disease diagnostics.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）