INORGANIC CHEMISTRY COMMUNICATIONS
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2024
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IF 4.4
Yujuan Sun,Jun Wu,Xiashi Zhu
A novel poly[1-vinyl-3-benzyltriethylammonium proline ionic liquid] membrane(PIL/PVDF membrane) were prepared through the blending poly[1-vinyl-3-benzyltriethylammonium proline ionic liquid](P[Pro][TEA])-poly(vinylidene fluoride)(PVDF) phase inversion method. A series of PIL/PVDF membrane characterization (NMR, FT-IR, SEM, EDS, and XPS) was performed. The properties of PIL/PVDF membrane (contact angle, water flux, mechanical properties, thermal stability,electric charge property, specific surface area and pore diameter) were evaluated. A solid phase extraction − high performance liquid chromatography (SPE-HPLC) method for separation/analysis of quercetin was developed. The factors affecting the adsorption and elution (time, dosage, pH etc.) were investigated. Under optimal conditions, the method has been successfully applied to the separation/analysis of quercetin in tea, and the recovery rates are satisfactory (86.4–106.0 %). The concentration factor of quercetin was 10. The adsorption capacity of PIL/PVDF membrane towards quercetin was 22.1 mg g −1 . The extraction mechanism was preliminarily discussed. The established SPE-HPLC method was simple and reliable, which provided application prospects for extraction and detection of quercetin.
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高影响因子
INTERNATIONAL IMMUNOPHARMACOLOGY
·
2024
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IF 4.8
Yao Wang,Jinjin Zhang,Yunsong Wang,Feng Wu,Baoshen Song,Jiatian Li,Qiuyue Lin,Yunpeng Xie,Yunlong Xia,Xiangbo An,Jiawei Liao
Aortic dissection (AD) is a life-threatening aortopathy with no specific pharmacological therapy. Ubiquitination, a highly orchestrated enzymatic cascade involving sequential E1-E2-E3 interactions, is suggested to contribute to the disease pathogenesis. However, the specific role of E1 enzymes in AD progression remains unknown. In this study, we analyzed the aortic transcriptional profiles of a human ascending dissection dataset (GSE52093) and identified ubiquitin-like modifier-activating enzyme 1 (UBA1) as a significantly up-regulated E1 enzyme in human AD. This finding was further corroborated by immunohistochemistry and RT-qPCR in a mouse model of AD induced by β-aminopropionitrile (BAPN). Treatment of TAK-243, a specific UBA1 inhibitor, prevented BAPN-induced AD formation in mice and attenuated aortic medial degeneration, as evidenced by decreased elastin fragmentation (evaluated by EVG scoring), reduced vascular smooth muscle cell loss (visualized by α-SMA immunohistochemistry), and less extracellular matrix degradation (indicated by diminished MMP2 and MMP9 expression in immunohistochemistry and RT-qPCR). Furthermore, TAK-243 treatment attenuated lesional macrophage accumulation and activation, as demonstrated by CD68 immunohistochemistry and RT-qPCR analysis of aortic pro-inflammatory cytokine expression. In vitro , UBA1 activation was observed in macrophages (RAW264.7 cells) treated with angiotensin II (AngII), and TAK-243 significantly reduced AngII-induced macrophage activation, at least partially through the inhibition of IκBα and NF-κB p65 phosphorylation. In conclusion, we demonstrate that UBA1 may facilitate AD progression by promoting macrophage activation via the NF-κB signaling pathway. These findings reveal a pathogenic role for the E1 enzyme UBA1 in AD and show a pharmacological potential of UBA1-targeted therapy against this disease.
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PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2024
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IF 12.2
Mingliang Zhang,Weihao Zhu,Hongfei Liu,Kaihua Pan,Qian Li,Qian Zhu,Yanni Huang,Changchang Wang,Junqiang Hu,Mingli Jiang,Xin Yan,Qing Hong
Acetaminophen (APAP), an analgesic and antipyretic drug, is commonly detected in wastewater treatment plant (WWTP) effluents, surface water, and soil, indicating its status as an emerging environmental contaminant. In this study, we isolated a bacterium, Pseudomonas taiwanensis AP-1, capable of completely mineralizing APAP and utilizing it as the sole carbon source for growth. A newly identified metabolite, γ-glutamyl-4-aminophenol (γ-G4AP), was reported for the first time in the degradation of APAP by strain AP-1. Two amidases (ApaH1 and ApaH2), responsible for the conversion of APAP to 4-aminophenol (4-AP), were identified through a combination of genomic comparison, heterologous expression, and gene knockout. Notably, ApaH1 played a pivotal role in the degradation of APAP by strain AP-1. The catalytic triad of ApaH1 (K82-S161-S185) and ApaH2 (K85-S160-S184) were identified as by molecular docking and site-directed mutagenesis. Additionally, a gene cluster apd for the metabolism of 4-AP was also successfully identified in strain AP-1, consisting of the aniline dioxygenase gene cluster apdBCD1D2EF and the BT catabolic gene apdGH . Interestingly, the 4-AP metabolic gene cluster apd was highly conserved among other Pseudomonas strains capable of APAP degradation. Our results provide new insights into the mechanism of APAP biodegradation and strain AP-1 may be a promising bacterium for the bioremediation of APAP pollutions.
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高影响因子
Small
·
2025
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IF 12.1
Zetao Chen,Weixia Li,Chuanlong Li,Songsong Huang,Yuhong He,Liyang Duan,Hui Luo,Jianqiong Zheng,Hongping Zhang,Chen Li,Cha Han,Bin Chen,Zi Lin,Shuang Li,Penghui Li,Furong Deng,Shike Hou,Liqiong Guo
Preeclampsia in pregnancies affects angiogenesis while the fetus develops. Early diagnosis of preeclampsia can enable timely prognosis and intervention. This study developed a smartphone-based, label-free dual-channel electrochemical biosensing platform for the simultaneous detection of preeclampsia biomarkers, placental growth factor (PLGF) and soluble Fms-like tyrosine kinase-1 (sFlt-1). Based on the functional modification of gold nanoparticles and reduced graphene oxide on working electrodes, the specific surface area is enlarged, and the stability is improved. PLGF and sFlt-1 antibody serving as sensitive elements, are immobilized on the electrode surface. As PLGF binds sFlt-1 dynamically, fluidic chambers are designed based on 3D printing for comprehensive detection. The biosensor achieved linear ranges of 10–8000 pg mL −1 for sFlt-1 and 1–250 pg mL −1 for PLGF, meeting clinical diagnostic requirements. Molecular docking is used to analyze the binding properties and signaling pathways of PLGF and sFlt-1, providing critical diagnostic insights for clinicians. This biosensing platform, featuring a portable integrated circuit and smartphone-based APP, enables point-of-care testing (POCT). Comparison tests using plasma samples from 10 individuals demonstrated a strong correlation (r > 0.98, p > 0.1) with ELISA, indicating the potential in clinical POCT applications for preeclampsia. These results suggest that the sensor can be used as a dual-channel biomarker for preeclampsia without sample labeling.
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PubMed
高影响因子
Frontiers in Pharmacology
·
2025
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IF 4.8
Ji Yang,Peng Guo,Hongtao Luo,Xin Tang,Wei Liu,Xiaolin Ren
Background: Current therapeutic options for inflammatory bowel disease (IBD) remain suboptimal due to limited efficacy, significant side effects, and high relapse rates, necessitating novel treatment strategies. Lumefantrine, a clinically established antimalarial drug, emerges as a compelling repurposing candidate based on its putative anti-inflammatory activity, though its efficacy and mechanism in IBD remain unexplored.Methods: A murine IBD model was induced by 3% dextran sulfate sodium (DSS). Mice received oral Lumefantrine (20 mg/kg/day) for 7 days. Disease progression was monitored via disease activity index (DAI) scoring and histological analysis. Serum cytokines (IL-1β, IL-6, TNF-α) and colonic inflammatory mediators (Cox-2, iNos) were quantified by ELISA and qPCR. Tight junction proteins (Claudin-1, ZO-1) were assessed by immunohistochemistry and Western blot. Molecular targets were identified through computational docking and pull-down assays. Additionally, NF-κB signaling modulation was assessed in lipopolysaccharide (LPS)-stimulated intestinal epithelial cells (IEC-6 and NCM460) via Western blot analysis.Results: Oral administration of Lumefantrine significantly attenuated disease activity index (DAI) scores and restored intestinal barrier integrity through upregulation of epithelial tight junction proteins Claudin-1 and ZO-1. Treated mice exhibited reduced serum levels of IL-1β, IL-6 and TNF-α, along with decreased colonic expression of inflammatory mediators cyclooxygenase-2 (Cox-2) and inducible nitric oxide synthase (iNos). Computational and experimental approaches identified FLI-1 a transcription factor upregulated in IBD colon tissues as Lumefantrine’s direct binding target. This interaction mediated suppression of NF-κB signaling, specifically downregulating phosphorylation of IκBα and p65 in LPS-stimulated intestinal epithelial cells.Conclusion: Lumefantrine ameliorates experimental colitis through FLI-1-dependent inhibition of the NF-κB pathway, demonstrating high repurposing potential as an IBD therapeutic.
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高影响因子
APPLIED SURFACE SCIENCE
·
2025
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IF 6.9
YuChen Wang,Changjian Liu,XinRui Liu,JingRu Han,LiJun Yang,Xiaoyuan Liao,Yue Yao
The photocatalytic production of hydrogen peroxide is a sustainable approach to producing oxygen and water. In this work, the microspheres flake-like, sheet-like, and flower-like BiOBr were by varying different solvents during the reaction process. The investigation revealed that the highest hydrogen peroxide production of 294 μmol g −1 h −1 was obtained for BiOBr(0 0 1). Their physico-chemical properties are fully investigated by XRD, SEM, XPS, ESR, and DFT calculation, which confirmed the exposure surface of BiOBr has a close relationship with the oxygen vacancy concentration, and BiOBr(0 0 1) has the highest oxygen vacancy concentration (oxygen vacancy formation energy 4.17 eV) and the best photocatalytic effect. DFT calculation also showed that the introduction of oxygen vacancies increased the adsorption and activation capacity of O 2 and greatly reduced the Gibbs free energy of the reaction pathway.
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高影响因子
INTERNATIONAL IMMUNOPHARMACOLOGY
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2025
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IF 4.7
Mengdan Shen,Xi Han,Xixi Li,Jiayun Ji,Shunyao Zhu,Beihua Xu,Senlin Shi,Ting Zhang
In this study, emodin (ED) and polysialic acid (PSA) were synthesized into a compound called PSA-ED. The PSA-ED self-assembled into nanoparticles (PE-NS) using a nano-precipitation method. According to the results of particle size analyzer, the particle size of PE-NS was 180.7 ± 1.60 nm, the polydispersity index (PDI) was 0.263 ± 0.090, and the ζ potential was −17.1 ± 0.9 mV. In vitro cellular experiments confirmed that compared to free emodin, PE-NS exhibited significantly higher cellular uptake in RAW264.7 cells. In addition, in vivo imaging demonstrated enhanced fluorescence accumulation in tumors. This indicated that PE-NS possesses the ability to effectively target tumor-associated macrophages (TAMs). We conducted experiments using a mouse model of the B16F10 tumor, treating the mice with PD-1 antibody, PE-NS, and various doses of PE-NS + anti-PD-1 antibody, and compared them with untreated controls. In the PE-NS and anti-PD-1 antibody combination therapy group, there was a notable decrease in the proportion of M2-TAMs and CD4 + Fop3+ T cells, alongside a significant increase in the proportion of M1-TAMs and CD8+ T cells, resulting in an increased tumor inhibition rate. The combination of PE-NS and anti-PD-1 antibody exhibited synergistic antitumor activity, enhancing the effectiveness of PD-1 checkpoint blockers in the mouse melanoma models. In conclusion, the combination of PE-NS and anti-PD-1 antibody immunotherapy holds great promise in improving the therapeutic effects of melanoma treatment and may offer a novel strategy for clinical application.
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高影响因子
International Journal of Nanomedicine
·
2025
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IF 6.5
Ren Na,Jianmei Jing,Hua Yang,Ye Li,Xiaofeng Yuan,Xue Sun,Jiangfan Han,Jiajun Wang,Zhenhua Tong,Guangbin He,Weiliang Ye
Objective Methotrexate (MTX) is widely used for rheumatoid arthritis (RA) but has poor targeting and significant side effects. This study developed MTX-loaded lipid nanoparticles modified with PVCAM-1 peptide (MTX@LNP-PVCAM-1) to enhance targeting and reduce toxicity.Methods MTX@LNP-PVCAM-1 was prepared using the thin-film dispersion method. Particle size and morphology were assessed by dynamic light scattering (DLS) and transmission electron microscopy (TEM). Biocompatibility was tested using human umbilical vein endothelial cells (HUVEC) and hemolysis assays. Cellular uptake was examined via fluorescence microscopy, while cytotoxicity and cell migration inhibition were evaluated using CCK-8 and scratch assays. Inflammatory cytokines (IL-1β, IL-6) were measured by ELISA. Distribution in adjuvant-induced arthritis (AIA) rats was observed using in vivo imaging, and safety and anti-inflammatory effects were assessed through blood tests, paw volume, joint scores, and histology.Results MTX@LNP-PVCAM-1 had an average particle size of 168.5 nm, PDI of 0.142, and zeta potential of −12.1 mV, with spherical morphology. It exhibited pH responsiveness and good biocompatibility. Compared with unmodified MTX@LNP, PVCAM-1 surface modification significantly increased cellular uptake efficiency (p<0.05) and more effectively inhibited the growth (p<0.05), migration (p<0.05), and secretion of inflammatory cytokines (significantly reduced levels of IL-1β and IL-6, p<0.05) of synovial fibroblasts. In animal experiments, the accumulation of MTX@LNP-PVCAM-1 in inflamed sites was significantly higher than that of MTX@LNP (p<0.05), demonstrating good targeting. Moreover, it enhanced the anti-inflammatory effects of methotrexate in AIA rats, significantly reducing paw swelling (p<0.05) and joint clinical scores (p<0.05). Importantly, it had no significant effect on the blood routine indicators of rats (p>0.05), indicating no obvious toxicity.Conclusion MTX@LNP-PVCAM-1 combines passive and active targeting, delivering MTX efficiently to inflamed sites and reducing toxicity. This approach enhances anti-inflammatory effects in AIA rats, offering a potential strategy for low-toxicity RA treatment.
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CHEMICAL ENGINEERING JOURNAL
·
2025
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IF 13.2
Cuihua Zhang,Yawen Yao,Wenqiu Xu,Jieying Zhou,Hua Yu,Ying Li,Wei Xiong
Efficient cytosolic delivery of small interfering RNA (siRNA) remains a challenge in RNA therapeutics, particularly for oral administration. Identifying new cytosolic stimulus-responsive targets is thus crucial for optimizing siRNA delivery. Given the elevated intracellular arginase-1 (Arg1) levels in inflamed colonic mucosa, we developed lipid nanoparticles (siRNA-LANPs) derived from L-arginine-modified chitosan (ACS) for the cytosolic release of TNF-α-siRNA to treat ulcerative colitis (UC). The siRNA-LANPs exhibited superior Arg1-binding affinity compared to its substrate, L-arginine, enabling rapid Arg1-responsive release. Moreover, we found that siRNA-LANPs maintained nanoparticle stability, and 84.5 % of the loaded siRNA remained intact in simulated digestive fluids over 12 h. Following oral administration, siRNA-LANPs efficiently penetrated the mucus layer and preferentially accumulated in inflamed colonic tissue compared to healthy colon tissue. In DSS-induced colitis mice, siRNA-LANPs significantly reduced disease severity through TNF-α silencing, achieving greater therapeutic efficacy than sulfasalazine (a first-line UC treatment). The siRNA-LANPs exhibited superior TNF-α silencing compared to transfection reagent Lipo3000 and TNF-α biologic agent infliximab. The enhanced performance was attributed to multiple endocytic pathways, improved lysosomal escape capability (29.7 %), and Arg1-mediated cytoplasmic siRNA release. In summary, Arg1 seems to be a promising intracellular target for stimulus-responsive, oral siRNA delivery in UC treatment.
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高影响因子
APPLIED SURFACE SCIENCE
·
2025
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IF 6.9
Lang Wu,Zhineng Zhou,Qiaogang Song,Na Yu,Xinghuan Hu,Ying Zhang,Xu Su,Xintong Zhao,Shurong Wang
SnO 2 has attracted significant attention as a potential replacement for TiO 2 in perovskite solar cells (PSCs) due to its high optical transmittance, stable physicochemical properties, and ability to process at low temperatures. However, when an SnO 2 electron-transport layer is prepared using solution gel spin coating, many oxygen vacancy defects are generated in the SnO 2 bulk and on its surface. These defects may result in nonradiative recombination at the SnO 2 /perovskite interface. This study proposes a novel and effective multibottom interfacial modification strategy that introduces 1,2-propanediamine hydroiodic acid (1,2-PDADI) molecules with multiple functional groups at the SnO 2 /perovskite interface. The incorporation of 1,2-PDADI molecules effectively passivates the oxygen vacancy defects at the SnO 2 surface and considerably improves the electronic properties of SnO 2 by reducing nonradiative recombination. Additionally, the interaction between the –NH 3 group in 1,2-PDADI and PbI 2 substantially reduces the PbI 2 content, thereby inhibiting defects forming in perovskite films. As a result, PSCs modified with 1,2-PDADI achieve a higher efficiency of 24.70% and demonstrate reduced hysteresis characteristics compared to the control (22.86 %). Thus, this study offers a cost-effective, straightforward, and effective approach for realizing high-efficiency PSCs.
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高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2025
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IF 6.2
Jing Huang,Siyi Chen,Weipeng He,Yinan Xiao,Nana Wang,Lili Huang
Phenazine-1-carboxylic acid (PCA), as a broad-spectrum antifungal agent, showed significant resistance against the Apple Valsa canker caused by Valsa mali (V. mali). Therefore, it is of great importance to reveal the functions and mechanisms of PCA in plant pathogens, which would provide potential targets for crop disease control. In this study, we identified the major contribution of PCA in the resistance of Pseudomonas chlororaphis subsp. aureofaciens W9-1 to V. mali by gene knockout and high-performance liquid chromatography. Subsequently, we demonstrated that PCA could target isocitrate lyase (ICL) to exert a broad-spectrum antifungal effect by molecular docking, microscale thermophoresis, and isothermal titration calorimetry. Furthermore, lipidomics analysis shows that PCA alters the lipid balance at the cell membrane of V. mali. In summary, this study demonstrates that PCA has a broad-spectrum antifungal effect by targeting the ICL, which may be a potential target for the development and utilization of fungicides.
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高影响因子
Joule
·
2024
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IF 38.6
Qiaonan Zhu,Dandan Yu,Jiangchun Chen,Liwei Cheng,Mengyao Tang,Yingyu Wang,Yuting Li,Jie Yang,Hua Wang
High-energy cryogenic batteries have been pursued throughout the past decades. Anode-free batteries offer a promising avenue to boost energy density because they comprise weightless anodes. However, their low-temperature operation is challenged by poor metal plating/stripping reversibility and unstable solid electrolyte interphase (SEI). Herein, the first low-temperature anode-free sodium battery is fabricated by simultaneous surface modification of the current collector and electrolyte design. The plasma-treated current collector (p-Al@C) possesses a sodiophilic N-doped carbon surface, enabling uniform sodium nucleation and growth; meanwhile, the optimized weak-solvation electrolyte facilitates Na + desolvation and the formation of a B/F inorganic-contained SEI. Na||p-Al@C half cells exhibit an ultrahigh average Coulombic efficiency of 99.88%, and p-Al@C||Na 3 V 2 (PO 4 ) 3 batteries achieve an energy density of 250 Wh kg −1 cathode + anode at −40°C. More interestingly, Ah-level anode-free pouch cells deliver a remarkable energy density of 110 Wh kg −1 entire cell at −40°C. This work represents a critical step for low-temperature sodium batteries.
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