Food Bioscience
·
2024
·
IF 4.8
Huanhuan Liu,Jiahui Li,Chunhong Yan,Jiaxiu Liu,Dongyun Zhao,Shugang Li,Xiaodong Xia
Inflammatory bowel disease is characterized by compromised epithelium barrier function and hyperactive intestinal inflammation. This study aims to investigate the effects of punicalagin, a bioactive ellagitannin in pomegranate, on dextran-sodium sulfate (DSS) - induced colitis in mice and to examine the underlying mechanisms involved. Oral administration of punicalagin (100 mg/kg/day) effectively mitigated the severity of experimental colitis in mice, evidenced by increased colon length, improved intestinal permeability, and decreased histological scores. Punicalagin up-regulated the mRNA expression level of Muc2 in and the protein expression of zonula occludens-1 (ZO-1) and Occludin in colitic mice. Moreover, punicalagin effectively decreased reactive oxygen species (ROS) levels in the colon, and in Caco-2 cells exposed to H 2 O 2 in vitro . Mechanistically, punicalagin attenuated inflammation by inhibiting the secretion of interleukin-6 (IL-6) and the phosphorylation of the NF-κB/STAT3 pathway. In addition, punicalagin increased adenosine monophosphate activated protein kinase (AMPK) phosphorylation in the colon of DSS-treated mice and lipopolysaccharide (LPS) -induced RAW 264.7 macrophages. Knocking down AMPKα by siRNA diminished punicalagin's anti-inflammatory function in RAW 264.7 macrophages exposed to LPS. Furthermore , molecular docking analysis, cellular thermal shift assay (CETSA), and drug affinity responsive target stability (DARTS) analysis supported the direct interaction between punicalagin and AMPK. In conclusion, punicalagin could mitigate DSS-induced colitis through enhancing intestinal epithelial barrier, alleviating oxidative stress, and attenuating inflammation partly through modulation of AMPK–NF–κB-STAT3 pathway.
研究领域:
药理学
·
免疫学
·
胃肠病学
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DOI
高影响因子
ChemistrySelect
·
2023
·Vol. 8
·期 33
·e202301702
·
IF 2.1
Zhiwei Chen,Xiaoxiao Huang,Luyao Yang
Graphical Lewis Acid-Mediated One-pot Tandem Reactions for synthesis of structurally diverse Furo[3,2-c]coumarins. The three-component cyanoalkylsulfonylation cyclization reaction of N -methacryloyl-2-phenylbenzimidazole, DABCO ⋅ (SO 2 ) 2 and cyclobutanone oxime ester regulated by different metals (iron and nickel) and the two-component cyanoalkylation cyclization reaction of DABCO ⋅ (SO 2 ) 2 as oxidant were described. The tandem cyclization route allows different cyanoalkylsulfonylated benzimidazole [2,1-a] iso-quinoline-6(5H)-ones/cyanoalkylated benzimidazole [2,1-a] iso-quinoline-6(5H)-ones to be obtained in moderate to good yields under mild conditions, with good functional group tolerance, readily available starting materials, and simple operation. In addition, experiments show that the tandem cyclization reaction was a free radical reaction process.
研究领域:
有机化学
·
药物化学
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DOI
高影响因子
JOURNAL OF GENE MEDICINE
·
2020
·Vol. 22
·期 9
·e3201
·
IF 3.258
Liping Ma,Yanjing Cao,Lin Zhang,Ketao Li,Laixing Yan,Yizhan Pan,Jianhua Zhu
Background Celastrol (Cel) has been corroborated as an anti-inflammatory and anti-apoptotic agent in multiple cell damage models. However, the protective effect of Cel in high glucose (HG)-induced cardiomyocyte injury is still unclear. The present study aimed to determine whether Cel can mitigate HG-stimulated cardiomyocyte injury via regulating the miR-345-5p/growth arrest-specific 6 (Gas6) signaling pathway. Methods Cardiomyocytes were exposed to normal glucose (NG; 5 mmol/l) or HG (30 mmol/l) and then administered with Cel. Cell counting kit-8 and flow cytometry assays were used to detect cell proliferative activity and apoptosis. mRNA and protein expression were analyzed using a quantitative reverse transcriptase-polymerase chain reaction and western blotting, respectively. A bioinformatics algorithm and a luciferase reporter gene assay were used to determine whether Gas6 is a direct target of miR-345-5p. Results The present study confirmed the inhibitory effects of Cel in HG-induced inflammation in cardiomyocytes. Moreover, Cel exhibited the ability to antagonize HG-induced cardiomyocyte apoptosis and suppress the elevated Bax/Bcl-2 ratio elicited by HG stimulation. Intriguingly, Cel treatment revoked the HG-triggered repression of Gas6 protein expression, and Gas6 loss-of-function accelerated HG-induced cardiomyocyte apoptosis. HG-triggered up-regulation of miR-345-5p expression was depressed following Cel treatment. Importantly, we validated that Gas6 is a direct target of miR-345-5p. Transfection with miR-345-5p inhibitors restrained HG-induced release of pro-inflammatory cytokines and cell apoptosis. Conclusions The findings of the present study demonstrate that Cel administration antagonized HG-induced cardiomyocyte apoptosis and inflammation through up-regulating Gas6 expression by restraining miR-345-5p.
研究领域:
心脏病学
·
分子生物学
·
药理学
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DOI
PubMed
高影响因子
TOXICOLOGY AND APPLIED PHARMACOLOGY
·
2020
·
IF 3.347
Yang Zhang,Jiabei Zhou,Qin Ye,Kui Zeng,Jie Pan,Lu Chen,Yingying Wang,Bo Yang,Qiaojun He,Jianqing Gao,Su Zeng,Lushan Yu
Thioredoxin 1 (Trx1) and telomerase play key roles in the development and progression process of most tumors, and they both are promising drug therapy targets. We have, for the first time, discovered that Trx1 and telomerase had a dual-target synergistic effect. Based on that results, we designed a series of 6-dithio-2′-deoxyguanosine analogs (named as YLS00X) and verified whether they can inhibit Trx1 and telomerase simultaneously. TrxR1/Trx1 system activity and telomerase expression were significantly inhibited by 6-dithio-2′-deoxyguanosine analogs, especially YLS004. YLS004 can also cause ROS accumulation, and induce tumor cell apoptosis . The vitro antitumor activity of 6-dithio-2′-deoxyguanosine analogs using MTT assay on 11 different human cancer cells and found that human colon cancer cells(HCT116) and melanoma cells (A375) were the most sensitive cells to 6-dithio-2′-deoxyguanosine analogs treatment and vivo xenografts models also confirmed that. The serum biochemical parameters and multiple organs HE staining results of subacute experiments indicated that YLS004 might be mildly toxic to immune organs, including the thymus, spleen, and hematopoietic system . Besides, YLS004 was rapidly metabolized in the rats' blood. Our study revealed that YLS004, a Trx1 and telomerase inhibitor , has strong anti-tumor effects to colon cancer and melanoma cells and is a promising new candidate drug.
研究领域:
肿瘤学
·
药理学
·
分子生物学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF LEGAL MEDICINE
·
2021
·Vol. 135
·期 2
·431-440
·
IF 2.686
Jiang Bowei,He Wei,Jin Chuan,Liu Ying,Wen Dan,Wang Chudong,ZEYE Moutanou Modeste Judes,Li Jienan,Zha Lagabaiyila
Genotyping of short tandem repeat (STR) markers is the basic method of forensic science. Enhanced technologies are needed to meet the requirements of databasing and casework samples. The STRscan-17LC kit is a 6 Dye STR kit which amplifies 16 STR loci: D3S1358, TPOX, D16S539, vWA, D2S1338, CSF1PO, D19S433, D7S820, FGA, D8S1179, D5S818, D13S317, D18S51, TH01, D12S391, and D21S11 and the sex-determinant locus amelogenin. This kit is designed for better tolerance to PCR inhibitors and analysis of mildly degraded samples with all fragments smaller than 330 bases. In this study, the STRscan-17LC kit is validated according to the SWGDAM (Scientific Working Group on DNA Analysis Methods) guidelines, including PCR-based studies, sensitivity, precision and accuracy, inhibitors, species specificity, DNA mixture studies, population, and concordance studies. The validation results suggest that the STRscan-17LC kit is a useful tool for forensic application.
研究领域:
法医学
·
分子生物学
·
遗传学
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DOI
PubMed
高影响因子
CHINESE JOURNAL OF POLYMER SCIENCE
·
2026
·
IF 4
Wu Jia-Le,Xiao Wen-Hao,Wu Jing-Bo,Lin Jiang-Hua,Xu Rui-Jie,Lei Cai-Hong
The chemical structure of polyamide 6 (PA6) dictates that only 50% of hydrogen bonds participate in crystallization during the crystallization process, resulting in the properties of its products being significantly dependent on the molding process. Therefore, the design and development of nucleating agents suitable for PA6 holds great practical significance for high-performance PA6 materials. Amide-based nucleating agents can effectively improve the crystallization rate by increasing intermolecular hydrogen bond density. Further introduction of hydroxyl groups can enhance the hydrogen bonding interactions between the nucleating agent and PA6. In this study, a hydroxyl-containing amide-based nucleating agent, BHT, was designed and synthesized using a tyramine-based biomass as the raw material. These results demonstrated that BHT exhibited good structural compatibility with PA6. After adding 1 wt% BHT, the crystallization temperature of PA6 increased from 170.9 °C to 193.3 °C, the crystallinity increased 16.6%, the heat distortion temperature and Vicat softening temperature rose to 89.5 and 187.8 °C, respectively, the haze decreased to 46%, achieving the synergistic optimization of mechanical, thermal, and optical properties. The in situ time-resolved FTIR results indicated that the addition of BHT increased the enthalpy of hydrogen bond formation during the nucleation stage, facilitated the segmental conformation adjustment of PA6, and enhanced the molar concentration of trans-conformations, ultimately leading to an improvement in the crystallization rate.
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产品关联
DOI
高影响因子
Journal of Physical Chemistry C
·
2017
·
IF 4.536
Wanfeng Li,Fang Zhang,Xingde Xiang,Xiucheng Zhang
Nickel-based ferrocyanides have attracted wide interest as cathode for aqueous sodium-ion batteries in fields of sustainable energies such as wind and solar, owing to excellent cycling stability and high-rate capability. However, they suffer from low specific capacities (∼60 mAh g–1). Herein, Na2Ni0.4Co0.6[Fe(CN)6] nanocrystallites are reported for the first time as high-capacity cathode for aqueous sodium-ion batteries. Its electrochemical properties and redox mechanism have been understood by combining the X-ray diffraction technique, Fourier-transform infrared spectroscopy, cyclic voltammetry, electrochemical impedance microscopy, and charge/discharge measurements. It is revealed that the material undergoes a reversible three-step single-phase reaction mechanism during Na extraction through sequential electrochemical oxidation of nitrogen-coordinated Co2+ ions and carbon-coordinated Fe2+ ions and achieves superior electrochemical performance with a high reversible capacity of 85 mAh g–1 at 0.5 C, an average operating potential of 0.62 V (vs Ag/AgCl), and a high capacity retention of 90% after 100 cycles. The combination of high specific energy and good cycling performance enables the Na2Ni0.4Co0.6[Fe(CN)6] material exhibiting promising application for high-performance aqueous sodium-ion batteries.
研究领域:
电化学
·
储能
·
材料科学
·
电池技术
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DOI
高影响因子
EUROPEAN JOURNAL OF PHARMACOLOGY
·
2026
·
IF 4.7
Wenying Fu,Ying Yan,Chongchong Shu,Chenxin Xu,Yinghua Chen,Yiqun Xia,Jundixia Chen,Yunzhi Chen,Ri Cui,Peng Zou,Daoyong Ni
6-Methoxydihydrosanguinarine is a natural alkaloid derived from medicinal plants that exhibits significant antitumor activity, making it a promising candidate for cancer therapy. However, the exact molecular mechanisms underlying its effects require further investigation. In this study, we investigated the cytotoxicity and underlying mechanisms of 6-Methoxydihydrosanguinarine in human non-small cell lung cancer (NSCLC) cells. Our findings reveal that reactive oxygen species (ROS) accumulation is the key driver of its antitumor activity. Mechanistically, 6-Methoxydihydrosanguinarine activates the JNK signaling pathway and induces endoplasmic reticulum (ER) stress, both of which can be reversed by the ROS scavenger N-acetylcysteine (NAC). Interestingly, 6-Methoxydihydrosanguinarine also activates autophagy, and inhibition of autophagy reverses the JNK and ER stress pathway activation induced by 6-Methoxydihydrosanguinarine. Notably, 6-Methoxydihydrosanguinarine synergistically enhances cisplatin-induced NSCLC cell death, and this synergistic effect is abolished by NAC, highlighting the critical role of ROS accumulation in their combined efficacy. This study systematically elucidates the molecular mechanisms of 6-Methoxydihydrosanguinarine against NSCLC, revealing that, in addition to the JNK and autophagy pathways, ER stress also mediates its antitumor effects. Moreover, our data establish a rationale for exploring 6-Methoxydihydrosanguinarine in NSCLC therapy and highlight its combination with cisplatin as a potentially effective strategy.
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产品关联
DOI
PubMed
高影响因子
POLYMER ENGINEERING AND SCIENCE
·
2026
·
IF 3.6
Wenqiao Meng,Kun Zhang,Wenxing Chen,Shichang Chen
Improving the toughness and reducing the oligomer content of Polyamide 6 through a green and sustainable bio-based modification strategy is of great significance for expanding its application potential. In this study, bio-based itaconic acid pentanediamine salt (IPA) was copolymerized with ε-caprolactam to prepare a bio-based copolyamide (PA6-IPA) containing pyrrolidone rings. The regulatory effects of IPA content on the oligomer content, thermal behavior, rheological properties, and mechanical properties of PA6-IPA were systematically investigated. The successful synthesis of PA6-IPA was confirmed by 1 H NMR and FT-IR. The results showed that with the increase of IPA content, the oligomer content of PA6-IPA decreased from 8.68% to 2.76%, the crystallinity crystallization temperature decreased significantly, and the relative content of crystal forms was regulated. In addition, PA6-IPA 20 achieved an elongation at break of 382.5% and a tensile strength of 58 MPa, realizing excellent toughness enhancement while maintaining the original strength. This can be attributed to the addition of IPA, which disrupts the regularity of the chain segments, increases the amorphous region of the copolyamide, and enhances the mobility of the molecular chains. This study provides a feasible strategy for developing eco-friendly, high-performance bio-based polyamides with balanced processability and mechanical properties. Highlights Bio-based PA6 copolymerized with itaconic acid-pentanediamine salt. The addition of IPA salt reduced the oligomers content from 8.68% to 2.76%. PA6-IPA 20 exhibited 382.5% elongation and 58 MPa strength.
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DOI
高影响因子
NATURAL PRODUCT RESEARCH
·
2026
·
IF 1.8
Qiao-Long Hao,Han-Mei Bai,Lei Miao,Shuang Yao,Chuan-Rui Zhang
The first phytochemical investigation of Tarlmounia elliptica (the sole species of its genus) led to the isolation of eight compounds from its aerial parts, including a previously undescribed seco-norlignan, tarlelliptin A (1), and a rare ring B aromatic 1(10→6)abeo-ergostane-type steroid, tarlelliptin B (2). Their structures were fully characterised by comprehensive spectroscopic analysis (HRESIMS, 1D/2D NMR) and corroborated by quantum chemical calculations (including ECD and NMR computations). Biological evaluation for cytotoxic, anti-inflammatory, and antibacterial activities indicated that only the known compound 7-oxo-β-sitosterol (8) exhibited weak antibacterial activity (MIC = 64.0 μg/mL) against methicillin-resistant Staphylococcus aureus (MRSA) strain 252.
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DOI
PubMed
高影响因子
Journal of Energy Storage
·
2026
·
IF 10.7
Kaixin Xie,Xiaobo Chen
In the pursuit of advanced energy storage solutions, materials featuring high porosity, robust redox activity, and abundant electrochemically accessible sites are highly attractive candidates for next-generation supercapacitor electrodes. In this study, a binder-free positive electrode material consisting of porous MnCo 2 O 4 particles directly coated onto carbon cloth (MnCo 2 O 4 @CC) was synthesized via a straightforward hydrothermal process followed by thermal treatment. Additionally, the incorporation of redox additives into electrolytes has been identified as a promising approach to elevate electrochemical supercapacitor performance. To this end, we designed an aqueous asymmetric supercapacitor (ASC) system utilizing MnCo 2 O 4 @CC and activated carbon (AC) electrodes, where potassium ferricyanide (K 3 [Fe(CN) 6 ], denoted as KFCN) was introduced as an electrolyte additive to augment pseudocapacitive behavior. The electron migration mechanisms and charge storage dynamics of MnCo 2 O 4 @CC in a KOH + KFCN electrolyte were systematically investigated. The MnCo 2 O 4 @CC electrode immersed in a 3 M KOH + 0.05 M KFCN solution exhibited a high specific capacitance of 2206.7 F g −1 at 1 A g −1 , along with low resistance characteristics and exceptional cycling durability. A complete aqueous MnCo 2 O 4 @CC//KOH + KFCN//AC ASC was assembled, demonstrating enhanced specific capacitance, extended cycle life (94.5% capacitance retention after 10,000 cycles), and an ultrahigh energy density of 79.9 Wh kg −1 at 799.6 W kg −1 . These findings provide significant electrochemical insights and demonstrate the great potential of the MnCo 2 O 4 @CC-KOH + KFCN system for next-generation supercapacitor applications.
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DOI
高影响因子
FOOD CHEMISTRY
·
2026
·
IF 10.4
Yuzhuo Wei,WenJing Guo,Shiqi Hao,Xueying Sun,Lina Sun,Erke Sun,Xiaotong Li,Ye Jin,Lihua Zhao
2-Amino-1-methyl-6-phenylimidazo [4,5-b] pyridine (PhIP) is a highly polar heterocyclic amine with elevated levels in high-temperature meat products and strong carcinogenicity. This study screened Pediococcus acidilactici X3-1B, achieving a PhIP adsorption rate of 74.30%. The stability of the complex formed between the strain and PhIP was highest at 0 h, 4 °C, and pH 7. Scanning electron microscopy (SEM) revealed significant morphological changes in the strain after PhIP adsorption. Fourier transform infrared spectroscopy (FTIR) results indicated that N H and C O groups on the bacterial surface played a primary role in adsorption. Zeta potential measurements revealed an increase in net negative charge on the bacterial surface following PhIP adsorption. Functional group chemical masking indicated that amino groups are one of the core functional groups influencing X3-1B's adsorption of PhIP. This study provides theoretical foundations and practical references for the application of lactic acid bacteria in food safety.
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DOI
PubMed
高影响因子
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