Analytical Methods
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2012
·Vol. 5
·期 4
·982-986
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IF 1.547
Mao Deng,Fu-nan Chen,Xing-yu Long
In the present work, a new method of chemiluminescence detection coupled with high performance liquid chromatography for three organic acids—tartaric, malic and citric acids is described. The detection is based on the enhancement of the organic acids for the chemiluminescence reaction between cerous sulfate (Ce(SO4)2) and tris (2,2′-bipyridyl)ruthenium(II) (Ru(bipy)32+). The latter was immobilized on the cationic ion-exchange resin for obtaining high sensitivity and reducing consumption of expensive reagent. Under the optimal conditions, the linear ranges were 7.5 × 10−7 g mL−1 to 7 × 10−6 g mL−1 for tartaric acid, 4.0 × 10−7 g mL−1 to 1.0 × 10−5 g mL−1 for malic acid and 1 × 10−7 g mL−1 to 1.5 × 10−6 g mL−1 for citric acid, and the detection limits were 1.5 × 10−7 g mL−1, 2.0 × 10−7 g mL−1, 4.0 × 10−8 g mL−1 for tartaric, malic and citric acid, respectively. The proposed method had been successfully applied to the determination of three organic acids in fruit juice – with only filtration and dilution steps.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2016
·
IF 2.857
Shuaihua Zhang,Qian Yang,Wenchang Wang,Chun Wang,Zhi Wang
A hybrid material of the zinc-based metal–organic framework-5 and graphene oxide (metal–organic framework-5/graphene oxide) was prepared as a novel fiber coating material for solid-phase microextraction (SPME). The SPME fibers were fabricated by covalent bonding via chemical cross-linking between the coating material metal–organic framework-5/graphene oxide and stainless steel wire. The prepared fiber was used for the extraction of five triazole fungicides from fruit and vegetable samples. Gas chromatography coupled with microelectron capture detector (GC-μECD) was used for quantification. The developed method gave a low limit of detection (0.05–1.58 ng g–1) and good linearity (0.17–100 ng g–1) for the determination of the triazole fungicides in fruit and vegetable samples. The relative standard deviations (RSDs) for five replicate extractions of the triazole fungicides ranged from 3.7 to 8.9%. The method recoveries for spiked fungicides (5, 20, and 50 ng g–1) in grape, apple, cucumber, celery cabbage, pear, cabbage, and tomato samples were in the range of 85.6–105.8% with the RSDs ranging from 3.6 to 11.4%, respectively, depending on both the analytes and samples. The metal–organic framework-5/graphene oxide coated fiber was stable enough for 120 extraction cycles without a significant loss of extraction efficiency. The method was suitable for the determination of triazole fungicides in fruit and vegetable samples.
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高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Lingcan Kong,Haixin Chen,Qiaoyan Liu
Excessive intake bioamines is harmful to human health, and thus the development of simple, rapid and efficient detection method of bioamines in food is urgent. Herein, an unprecedented method based on a commercial molecular sieve has been developed for the determination of four bioamines (cadaverine, tyramine, histamine and tryptamine) in fruit wines by dispersive solid phase microextraction (D-μSPE) coupled to liquid chromatography-triple quadrupole tandem mass spectrometry (LC-MS/MS). The extraction process of ultra-stable Y-type molecular sieve (USY) on bioamines belongs to physisorption, and the adsorption pattern is found in good accord with the Langmuir isotherm model. Theoretical and experimental analyses are further used to explore their binding energies, binding constants, and the maximum adsorption capacity. It is found that the adsorption is driven mainly by hydrogen bonds between USY and bioamines and water molecules. Moreover, the extraction efficiency is dependent on the binding constants and the maximum adsorption capacity of USY. The optimization of adsorption-desorption process involves the USY amount, extraction time, desorption solvent, desorption time, desorption number and cyclic usage number of USY. Under the optimum conditions, the limits of detection and quantification were found in the range of 0.2–2.5 μg L −1 and 0.6–8 μg L −1 with intra-day and inter-day precision and recoveries in a fruit wine in the range of 1.3 %–7.9 % and 91 %–104 %, respectively. Owing to the efficient enrichment and edulcoration functions of USY, the method shows negligible matrix effect and is simple, rapid, and has the potential to apply in routine work.
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FOOD CHEMISTRY
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2020
·
IF 6.306
Ibrahim Abdelhai Senosy,Hao-Ming Guo,Mei-Nan Ouyang,Zhi-Heng Lu,Zhong-Hua Yang,Jian-Hong Li
In this work, zeolite imidazolate framework-8 (ZIF-8) functionalized magnetic graphene oxide (Fe 3 O 4 @APTES-GO/ZIF-8) was successfully synthesized and used as a novel adsorbent in magnetic solid-phase extraction (MSPE) for the determination of four triazole fungicides in water, honey and fruit juices. The main parameters such as extraction time, amount of adsorbent, the pH value of the sample, ionic strength, and desorption solvent which could affect the experiment results were optimization. Under the optimum condition, the obtained linearity of this method ranged from 1 to 1000 µg L −1 for all analytes, with correlation coefficients (R 2 ) ≥ 0.9914. Limit of detections (LODs) and limit of qualifications (LOQs) of four triazole fungicides were ranged from 0.014 to 0.109 µg L −1 and from 0.047 to 0.365 µg L −1 , respectively. Based on comparison with outcomes from other studies, Fe 3 O 4 @APTES-GO/ZIF-8-MSPE could provide high performance and achieve satisfied results for the analysis of trace triazole fungicides in complicated matrices.
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JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
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2024
·
IF 3.3
Xiefei Li,Yupeng Lin,Yumeng Huang,Xin Li,Fengping An,Hongbo Song,Qun Huang
BACKGROUND Pectin extracted by high-speed shearing from passion fruit peel (HSSP) is a potentially excellent wall material for encapsulating curcumin, which has multiple advantages over pectin prepared by heated water extraction. HSSP was used to fabricate complex nanoparticles of zein–sodium caseinate–pectin for encapsulation of curcumin in this study. The influence of heating on the physicochemical properties of the composite nanoparticles was also investigated, as well as the effect of composite nanoparticles on the encapsulation efficiency, antioxidant activity and release characteristics of curcumin. RESULTS The nanoparticles were formed through electrostatic interactions, hydrogen bonds and hydrophobic interactions between the proteins and HSSP. A temperature of 50 °C was more favorable for generating compact and small-sized nanoparticles, which could effectively improve the encapsulation efficiency and functional properties. Moreover, compared to other pectin used in the study, the nanoparticles prepared with HSSP showed the best functionality with a particle size of 234.28 ± 0.85 nm, encapsulation rate of 90.22 ± 0.54%, free radical scavenging rate of 78.97% and strongest protective capacity in simulated gastric fluid and intestinal release effect. CONCLUSION Zein–sodium caseinate–HSSP is effective for encapsulating and delivering hydrophobic bioactive substances such as curcumin, which has potential applications in the functional food and pharmaceutical industries. © 2024 Society of Chemical Industry.
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高影响因子
Foods
·
2025
·Vol. 14
·期 10
·1707
·
IF 5.1
Li Li,Ying Hu,Yu-Mei Wang,Xiao-Xue Wu,Si-Tong Lin,Hang Li,Ji Zhang,Guo-Rong Fan,Zong-De Wang,Bin Wang,Shang-Xing Chen
In this study, we systematically investigated the mechanisms of the antioxidation and anti-lipid accumulation effects of antioxidant peptides fromLitsea cubebaon a free fatty acid (FFA)-induced NAFLD model of HepG2 cells. The NAFLD cell model was constructed by inducing the HepG2 hepatocellular carcinoma cell line with 0.5 mmol/L FFAs, and AQRDAGLL, QEGPFVR, and DVPPPRGPL were given to the culture to study their lipid-lowering and antioxidant activities on NAFLD cells. The lipid-lowering activities of the three antioxidant peptides were evaluated by Oil Red O staining and TG and TC content assays, and the results showed that all three peptides had strong ameliorating effects on FFA-induced lipid accumulation in NAFLD cells. The intracellular antioxidant protease (CAT, GSH, and SOD) activity levels and lipid peroxidation (MDA) content were measured and intracellular ROS levels were detected. The results showed that after intervention with the antioxidant peptides, the intracellular ROS levels in the NAFLD model cells were significantly reduced, the SOD and CAT activities were increased, the GSH content was elevated, and the MDA content was reduced, which indicated that AQRDAGLL, QEGPFVR, and DVPPPRGPL were able to inhibit the oxidative stress of the cells effectively and to achieve the effect of intervening in NAFLD. JC-1 fluorescence staining experiments showed that the mitochondrial membrane potential function of NAFLD cells was restored under the effect of the antioxidant peptides. Molecular dynamics simulations revealed that the main driving force between QEGPFVR and Keap1 protein was van der Waals forces, ΔG = −62.11 kcal/mol, which indicated that QEGPFVR was capable of spontaneously binding to Keap1 protein.
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高影响因子
Polymers
·
2022
·Vol. 14
·期 3
·536
·
IF 4.967
Muhammad Rafiullah Khan,Chongxing Huang,Rafi Ullah,Hakim Ullah,Ihsan Mabood Qazi,Taufiq Nawaz,Muhammad Adnan,Abdullah Khan,Hongxia Su,Liu Ren
Plant extracts represent a rich repository of metabolites with antioxidant and antimicrobial properties. Neem (Azadirachta indica) is a medicinal plant considered the tree of the 21st century. In this study, we investigated the antioxidant and antimicrobial effects of propyl disulfide (PD), a major volatile compound in neem seed, against the pericarp browning (BI), microbial decay incidence (DI), and water loss of longan fruit. Fresh longan cv. Shixia samples were packaged in oriented polypropylene (OPP) and polyethene (PE) packages of different thicknesses (20, 40, and 60 µm). Sterile gauze was fixed inside the packages and 500 uL of PD was placed on them to avoid the direct contact of PD with fruit samples. Packages were sealed immediately to minimize vaporization and stored at 12 ± 1 °C for 18 days. Fruit samples packaged in open net packages served as controls. The results showed that fruit treated with PD in OPP and PE packages significantly prevented losses of water, DI, and BI compared to control treatment. PD also maintained the color, TSS values, TA values, pH values, high peel firmness, high TPC content, and high TFC content, and reduced the activity levels of PPO and POD. Scanning electron microscope (SEM) analysis indicated that the exocarp, mesocarp, and endocarp of longan peel were smooth, uniform, and compact with no free space compared to control, where crakes, a damaged and loose structure, and a lot of fungal mycelia were found. The shortest shelf life of 9 days was observed in control as compared to 18 days in OPP-20 and OPP-40; 15 days in OPP-60, PE-20, and PE-40; and 12 days in PE-60 packaging films. Therefore, PD as a natural antioxidant and antimicrobial agent, in combination with OPP-20 and OPP-40 polymeric films, could successfully be applied commercially to extend the postharvest shelf life of longan.Keywords:longan;fruit;polymeric films;antioxidant activity;enzymatic browning;neem;propyl disulfide;microbial decay;essential oil
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高影响因子
Fermentation-Basel
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2023
·Vol. 9
·期 5
·432
·
IF 3.7
Yuhao Sun,Zhenzhen Wang,Jing Dai,Ruyi Sha,Jianwei Mao,Yangchen Mao,Yanli Cui
In this work, spontaneously fermented and inoculation-fermentedAkebia trifoliatafruit Jiaosu (SFAJ/IFAJ) were compared. The key metabolites and antioxidant activities of SFAJ and IFAJ were tracked and tested during fermentation. The antioxidant effect of fermentedAkebia trifoliatafruit and the underlying mechanisms were explored using network pharmacology for the prediction and verification of the molecular targets and pathways of theAkebia trifoliatafruit’s action against oxidative stress. Furthermore, the results were verified by molecular docking and then investigated, based on a HepG2 cell model. The results of correlation analysis and principal component analysis (PCA) showed that there were significant positive correlations between the phenols, flavonoids, and terpenoids in SFAJ and IFAJ and their antioxidant activities. Network pharmacology and molecular docking analysis disclosed the antioxidation mechanism at the molecular level. In addition, both SFAJ and IFAJ were effective at alleviating oxidative stress in HepG2 cells. In particular, IFAJ performed better than SFAJ in protecting cells with an intracellular reactive oxygen species (ROS) level of 99.96 ± 4.07 U/mg prot, superoxide dismutase (SOD) activity of 41.56 ± 0.06 U/mg prot, catalase (CAT) activity of 91.78 ± 3.85 U/mg prot, and glutathione peroxidase (GSH-Px) activity of 39.32 ± 2.75 mU/mg prot in the IFAJ group. Collectively, this study revealed the changes in bioactive metabolite contents and the in vitro antioxidant activity during fermentation and investigated the protectiveness of SFAJ and IFAJ against oxidative stress within HepG2 cells, promoting the study of the antioxidant efficacy of IFAJ, thereby providing valuable reference data for the optimization of its preparation and the development of relevant products with health benefits.Keywords:Akebia trifoliatafruit;bioactive metabolites;antioxidant activity;network pharmacology;molecular docking;oxidative stress
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NEW JOURNAL OF CHEMISTRY
·
2019
·Vol. 43
·期 32
·12563-12569
·
IF 3.069
Huixiao Duo,Xiaofeng Lu,Shuai Wang,Licheng Wang,Yong Guo,Xiaojing Liang
Herein, a novel, fusiform-like magnetic metal–organic framework material (Fe3O4-NH2@MOF-235) was fabricated by a facile two-step solvothermal approach. Then, Fe3O4-NH2@MOF-235 was applied as an adsorbent in magnetic solid-phase extraction (MSPE) coupled with high-performance liquid chromatography (HPLC) for the simultaneous extraction and determination of five kinds of benzoylurea insecticides (flufenoxuron, chlorbenzuron, teflubenzuron, diflubenzuron and triflumuron) from honey, fruit juice and tap water samples. Moreover, potential variables influencing the MSPE efficiency were optimized. Under the optimized conditions, an excellent linearity was achieved in the range of 1.0–300.0 μg L−1 for teflubenzuron and flufenoxuron and 2.0–300.0 μg L−1 for diflubenzuron, chlorbenzuron and triflumuron, with the correlation coefficients of 0.9991–0.9995. The limits of detection for the analytes were 0.25–0.5 μg L−1, and the recoveries at three fortified concentration levels ranged from 76.45% to 90.74% for the honey samples, 79.95–95.27% for the fruit juice samples, and 84.02–99.62% for the tap water samples. The π–π stacking and hydrophobic interactions between Fe3O4-NH2@MOF-235 and the analytes played an important role in the adsorption process. Moreover, maximum adsorption could be achieved within 5 minutes with very little adsorbent for all the samples. Therefore, the magnetic Fe3O4-NH2@MOF-235 composite combined with MSPE is a convenient approach to detect benzoylurea insecticides in actual samples.
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JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
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2013
·
IF 2.487
Pei Liang,Guojiao Liu,Fang Wang,Wanting Wang
A novel method, ultrasound-assisted surfactant-enhanced emulsification microextraction with solidification of floating organic droplet (UASEME-SFOD), has been developed for the extraction of four strobilurin fungicides (kresoxim-methyl, picoxystrobin, pyraclostrobin and trifloxystrobin) in fruit juices. In the UASEME-SFOD technique, Tween 80 was used as emulsifier, and 1-undecanol was used as extraction solvent without using any organic dispersive solvent. Several parameters that affect the extraction efficiency, such as the kind and volume of extraction solvent, the type and concentration of the surfactant, extraction time, extraction temperature and salt addition were investigated and optimized. Under the optimum extraction condition, the method yields a linear calibration curve in the concentration range from 5 to 10,000 ng mL −1 for the targeted analytes with the correlation coefficient ranging from 0.9991 to 0.9998. The enrichment factors were in the range between 95 and 135, and the limits of detection of the method were 2–4 ng mL −1 . The fruit juice samples were successfully analyzed using the proposed method, and the relative recoveries at fortified levels of 50 and 100 ng mL −1 were in the range of 82.6–97.5%.
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高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Haodong Wang,Cancan Qian,Lanlan Sun,Hao Jiang,Xiaodong Wang,Jianghu Cui,Desong Yang
The use of nano-pesticide delivery systems to suppress crop disease has great potential in sustainable agriculture. Herein, a pH-responsive salicylic acid (SA) conjugation based on aminopropyl-functionalized ZnO quantum dots (SA-ZnO QDs) with a loading capacity of 13.4 wt% was prepared by an amidation reaction. Results indicated that SA-ZnO QDs could release SA rapidly in an acidic condition, which corresponded to the pH of the water-soaked lesions where the bacterial fruit blotch disease often spread. The antibacterial activity of the synthesized SA-ZnO QDs was about 1.6 times and 1.3 times higher than that of SA and aminopropyl-functionalized ZnO QDs, respectively. A pot experiment showed that foliar application of SA-ZnO QDs could reduce the incidence of bacterial fruit blotch disease (54.46%) by increasing the antioxidant defense systems (44.33–79.59%) and nutrient absorption (23.19–26.95%). More importantly, we observed that, after the application of SA-ZnO QDs, the systemic acquired resistance and flavone biosynthesis were activated in infected melon seedlings, which was revealed by metabolomics. This work demonstrates the important role of the quantum-delivery system in improving crop disease suppression for sustainable agriculture.
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高影响因子
International Journal of Refrigeration
·
2025
·
IF 3.8
Shiya Lin,Yiqi Xie,Xianyang Chen,Pengcheng Lin,Ying Chen
Advanced cold chain systems are essential for maintaining nutritional integrity and reducing spoilage in perishable fruits. However, conventional mechanical refrigeration incurs considerable energy consumption and operational costs. This study introduces a green, safe, and cost-effective alternative—Brine Phase Change Cold Storage Gels (BPCCSGs). With a low phase change temperature (−11.29 °C), high latent heat (242.14 J·g⁻¹), and elevated thermal conductivity (0.835 W·m⁻¹·K⁻¹), BPCCSGs are well-suited for cold chain applications involving subtropical fruits. Additionally, the risk of cold storage material leakage and its consequent detriment to fruit safety could be significantly reduced by using our state-of-the-art gel packaging technique. Gel-based encapsulation effectively minimized leakage risk and prevented potential contamination. Pre-charging BPCCSGs during off-peak hours significantly improved energy and cost efficiency (3.82 × 10⁻⁵ $·J⁻¹), while reducing environmental burden. Moreover, the improvement in thermal conductivity and the optimization of the spatial layout facilitated temperature uniformity of BPCCSGs at cold storage scenarios. Finally, the integration of a 5G-enabled sensor system enabled real-time monitoring of temperatures and locations. The system was successfully deployed in waxberry logistics, ensuring real-time traceability and maintaining fruit quality, highlighting its potential for next-generation cold chain systems.
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危险品化学品经营许可证（带存储）