JOURNAL OF PROSTHETIC DENTISTRY
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2022
·
IF 4.148
Hao Yang,Xianju Xie,Xiaowei Li,Yuxing Bai
Statement of problem Polymethyl methacrylate (PMMA) is commonly used in dentistry, including as a denture base material. However, the colonization of a PMMA surface by microbial microorganisms could increase the risk of oral diseases such as denture stomatitis and gingivitis. The development of PMMA with antibacterial properties should improve its clinical application, but whether adding ε-poly-L-lysine (ε-PL) and 2-methacryloyloxyethyl phosphorylcholine (MPC) provides antimicrobial effects is unclear. Purpose This in vitro study aimed to develop a novel antibacterial PMMA resin containing the natural nontoxic antibacterial agent ε-PL and the protein repellent agent MPC. The mechanical properties, protein repellency, and antimicrobial activities of the resin were then evaluated. Material and methods Different mass fractions of ε-PL and MPC were mixed into PMMA as the experimental groups, with unaltered PMMA as the control group. The flexural strength (n=10) and surface roughness (n=6) of the resulting mixtures were measured to determine their mechanical properties. The antiprotein properties were measured by using the micro bicinchoninic acid method (n=6). The antimicrobial effect of the resin was assessed using live/dead staining (n=6) and methyltransferase (MTT) assays (n=10). According to the variance homogeneity and normal distribution results, 1-way analysis of variance followed by the Tukey honestly significant difference test or the Welch test and the Games-Howell test were used (α=.05 for all tests). Results No significant differences were found in the flexural strength values and surface roughness of the specimens containing 1.5% MPC and 1.5% ε-PL compared with those of the control ( P >.05). The addition of ε-PL to the PMMA resin alone significantly increased its bactericidal properties ( P <.05). Adding both ε-PL and MPC further increased the antibacterial activity of the PMMA resin without increasing protein adhesion more than in the control group. Conclusions The incorporation of both ε-PL and MPC into PMMA improved its antibacterial capacity without affecting its mechanical properties and did not increase protein adhesion. Therefore, the novel PMMA fabricated in this study shows promise for dental applications.
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高影响因子
LETTERS IN APPLIED MICROBIOLOGY
·
2015
·Vol. 61
·期 4
·390-396
·
IF 1.659
Chao H.,Wu B.,Shen P.
Increase in L‐serine production is of interest for industry. Here, we describe a metabolic engineering approach to increase the production of L‐serine in a methylotrophic bacterium. mxaF, the gene encoding the large subunit of a methanol dehydrogenase, was cloned from Methylobacterium sp. MB200 through transposon mutagenesis. Deletion of mxaF gene prevented the strain to grow on methanol, suggesting that mxaF is involved in methanol metabolism. Overexpression of mxaF gene in the strain MB200 resulted in a fivefold increase in methanol dehydrogenase activity compared to the wild‐type. Resting cell assays showed that the recombinant strain accumulated 6·6 mg ml−1 L‐serine in 72 h with 30 mg ml−1 wet cells from 50 mg ml−1 glycine and 50 mg ml−1 methanol, representing a 1·5‐fold increment for L‐serine production in contrast to the wild‐type strain. These results demonstrate that the potential for improving the production of L‐serine can be achieved by overexpressing mxaF gene in methylotrophic bacteria.Significance and Impact of the StudyThe amount of L‐serine produced each year worldwide is relatively small compared with the amounts of the other amino acids and hence it is in great demand. Here, we describe a metabolic engineering approach to increase the production of L‐serine in a methylotrophic bacterium Methylobacterium sp. MB200. The result demonstrates that raising the output of L‐serine can be achieved by overexpressing mxaF gene in methylotrophic bacteria.
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PeerJ
·
2023
·
IF 2.7
Wanwei Hou,Xiaojuan Zhang,Yuling Liu,Yujiao Liu,Bai li Feng
Background Faba bean ( Vicia faba L) is one of the most important legumes in the world. However, there is relatively little genomic information available for this species owing to its large genome. The lack of data impedes the discovery of molecular markers and subsequent genetic research in faba bean. The objective of this study was to analyze the faba bean transcriptome, and to develop simple sequence repeat (SSR) markers to determine the genetic diversity of 226 faba bean varieties derived from different regions in China. Methods Faba bean varieties with different phenotype were used in transcriptome analysis. The functions of the unigenes were analyzed using various database. SSR markers were developed and the polymorphic markers were selected to conduct genetic diversity analysis. Results A total of 92.43 Gb of sequencing data was obtained in this study, and 133,487 unigene sequences with a total length of 178,152,541 bp were assembled. A total of 5,200 SSR markers were developed on the basis of RNA-Seq analysis. Then, 200 SSR markers were used to evaluate polymorphisms. In total, 103 (51.5%) SSR markers showed significant and repeatable bands between different faba bean varieties. Clustering analysis revealed that 226 faba bean materials were divided into five groups. Genetic diversity analysis revealed that the relationship between different faba beans in China was related, especially in the same region. These results provided a valuable data resource for annotating genes to different categories and developing SSR markers.
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Frontiers in Bioengineering and Biotechnology
·
2021
·
IF 5.89
E Yang,Meng Jun,Cai Heqing,Li Caibin,Liu Sainan,Sun Luming,Liu Yanxiang
The organic compounds from biochar play a role of hormone analogs, stimulating the expression of metabolites by controlling related gene and protein. In this experiment, we reported the L-histidine biosysthesis was promoted by biochar treatment in E. coli unlike genetic engineering of the traditional method. The related results indicated the most optimal concentration was found to be 3%, and 7% is the lethal dose. E. coli was inhibited in the high-concentration treatment. On the other hand, docking technology was usually used as drug screening, basing on Lock-and-key model of protein in order to better understand mechanisms. So the organic compounds of biochar from GC-MS analysis that acted as ligands were connected to HisG protein controlling L-histidine biosysthesis in E. coli. The result showed that the three organic molecules interacted with HisG protein by hydrogen bond. So we considered that these three compounds play regulatory roles in L-histidine biosysthesis, and the hisG gene expression fully supports this conclusion.
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高影响因子
Nanomaterials
·
2021
·Vol. 12
·期 1
·6
·
IF 5.076
Mingchao Sun,Shaojuan Chen,Peixue Ling,Jianwei Ma,Shaohua Wu
Electrospun nanofiber mats have attracted intense attention as advanced wound dressing materials. The objective of this study was to fabricate methacrylated gelatin (MeGel)/poly(L-lactic acid) (PLLA) hybrid nanofiber mats with an extracellular matrix (ECM) mimicking nanofibrous structure and hydrogel-like properties for potential use as wound dressing materials. MeGel was first synthesized via the methacryloyl substitution of gelatin (Gel), a series of MeGel and PLLA blends with various mass ratios were electrospun into nanofiber mats, and a UV crosslinking process was subsequently utilized to stabilize the MeGel components in the nanofibers. All the as-crosslinked nanofiber mats exhibited smooth and bead-free fiber morphologies. The MeGel-containing and crosslinked nanofiber mats presented significantly improved hydrophilic properties (water contact angle = 0°; 100% wettability) compared to the pure PLLA nanofiber mats (~127°). The swelling ratio of crosslinked nanofiber mats notably increased with the increase of MeGel (143.6 ± 7.4% for PLLA mats vs. 875.0 ± 17.1% for crosslinked 1:1 MeGel/PLLA mats vs. 1135.2 ± 16.0% for crosslinked MeGel mats). The UV crosslinking process was demonstrated to significantly improve the structural stability and mechanical properties of MeGel/PLLA nanofiber mats. The Young’s modulus and ultimate strength of the crosslinked nanofiber mats were demonstrated to obviously decrease when more MeGel was introduced in both dry and wet conditions. The biological tests showed that all the crosslinked nanofiber mats presented great biocompatibility, but the crosslinked nanofiber mats with more MeGel were able to notably promote the attachment, growth, and proliferation of human dermal fibroblasts. Overall, this study demonstrates that our MeGel/PLLA blend nanofiber mats are attractive candidates for wound dressing material research and application.Keywords:electrospinning;hydrogel;methacrylated gelatin;poly(L-lactic acid);wound dressing
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Materials Science & Engineering C-Materials for Biological Applications
·
2012
·
IF 2.686
Shen Liu,Jingwen Zhao,Hongjiang Ruan,Wei Wang,Tianyi Wu,Wenguo Cui,Cunyi Fan
The complications of tendon injury are frequently compromised by peritendinous adhesions and tendon sheath infection. Physical barriers for anti-adhesion may increase the incidence of postoperative infection. This study was designed to evaluate the potential of silver nanoparticles (AgNPs)-loaded poly( l -lactide) (PLLA) electrospun fibrous membranes to prevent adhesion formation and infection. Results of an in vitro drug release study showed that a burst release was followed by sustained release from electrospun fibrous membranes with a high initial silver content. Fewer fibroblasts adhered to and proliferated on the AgNP-loaded PLLA electrospun fibrous membranes compared with pure PLLA electrospun fibrous membrane. In the antibacterial test, the AgNP-loaded PLLA electrospun fibrous membranes can prevent the adhesion of Gram-positive Staphylococcus aureus and Staphylococcus epidermidis and Gram-negative Pseudomonas aeruginosa . Taken together, these results demonstrate that AgNP-loaded PLLA electrospun fibrous membranes have the convenient practical medical potential of reduction of infection and adhesion formation after tendon injury.
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CHEMICAL ENGINEERING JOURNAL
·
2021
·
IF 13.273
Lin Sun,Yu Liu,Ming Yan,Qingjun Yang,Xiaoying Liu,Weidong Shi
The ideas of high effective specific surface area, multi-component and multi-phase synergistic are introduced to construct high-performance and high-stability electrodes. After etching and exchanging with Lewis acid (nickel ion) and annealing selenization, the hierarchical multi-phase Ni x Co 1-x Se 2 /carbon–nitrogen frameworks (CNFs, Zeolitic-Imidazolate-Framework-L derivatives) on CoO is synthesized. After systematic research, it is found that the concentration and reaction time of the Lewis acid and selenization conditions can directly affect the structure and composition (bimetal) of the electrode. In the optimal electrode, CNFs with the larger effective specific surface area can fully expose the active material to the electrolyte, the bimetal synergy (Ni-Co), and the multiphase synergy ((Ni, Co)Se 2 and CoSe 2 ) can effectively enhance the performance. The anode demonstrates a high mass-specific capacity of 207.8 mAh g −1 . A hybrid supercapacitor (Ni x Co 1-x Se 2 /CNFs/CoO@CC//activated carbon) is fabricated and displayed superior performance and stability: high energy density (45.0 Wh kg −1 , 800.0 W kg −1 ), and a cycle retention rate of 98.0% (12,000 cycles). LEDs in parallel were lit up by the devices, showing their practical value and potential.
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REACTIVE & FUNCTIONAL POLYMERS
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2020
·
IF 3.333
Yu Tang,Qinya Wang,Lexuan Wu,Kuan Liu,Wenjing Wang,Yinghua Shen,Yuyuan Xue,Sheng Dai
pH-Responsive copolymers with reversible transformation ability have been investigated as new effective carriers for L-proline to catalyze asymmetric aldol reaction in water. A series of block copolymers of mPEG- b -P(DEA x - co -L-ProlA y ) (mPEG-PDL) bearing L-proline catalytic sites and pH-responsive poly (diethylaminoethyl methacrylate) (PDEA) segments were synthesized by the reversible addition-fragmentation chain transfer (RAFT) polymerization in the presence of methyl polyethylene glycol (mPEG) trithiocarbonate macro-RAFT agent. The pH-induced self-assembly behaviors of three copolymer catalysts with different contents of PDEA were investigated via the combination of UV–visible spectrometer (UV–vis), dynamic light scattering (DLS) and transmission electron microscopy (TEM). Catalytic activity and stereoselectivity of these copolymer catalysts were evaluated by direct asymmetric aldol reaction of p -nitrobenzaldehyde and cyclohexanone in water. The results indicated that catalytic activity and stereoselectivity effected by the pH value of aqueous solution and the structures of copolymer catalyst assemblies. mPEG-PDL-2 give the aldol product with high conv., yield, anti/syn , and ee (92%, 91%, 92/8 , 96%), respectively at pH 7.0. Furthermore, these pH-responsive copolymers supported L-proline are easily recycled by a reversible phase transition.
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NEW JOURNAL OF CHEMISTRY
·
2019
·Vol. 43
·期 8
·3323-3331
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IF 3.069
Xi Wang,Tian Gao,Mian Yang,Jie Zhao,Feng-Lei Jiang,Yi Liu
L-Asparagine, a common amino acid, is a cost-effective and ideal precursor for carbon dots because of its high nitrogen content and its abundance in nature. By using L-asparagine as a biocompatible precursor, fluorescent nitrogen-doped carbon dots (A-CDs) were prepared by a one-pot microwave-assisted method. Then, a reasonable structure for A-CDs was put forward, which confirmed that unconjugated small molecules could transform into carbon dots with the sp2 core, and was helpful in the discussion of the photoluminescence mechanism. It was concluded that the photoluminescence origin of A-CDs was from the graphite-like structure and surface defects. The A-CDs showed high stability in strong ionic solutions and negligible cytotoxicity even when the incubation concentration was up to 800 μg mL−1. Long-time bioimaging tests suggested that A-CDs have great potential for use in a wide variety of biological applications.
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Scientifica
·
2020
·
IF 0
Jasvidianto C. Kotta,Agatha B. S. Lestari,Damiana S. Candrasari,Maywan Hariono
Goat weed (Ageratum conyzoides L.), or bandotan in Indonesia, is an herbaceous plant that broadly grows up in both subtropical as well as tropical areas. This herb contains many phytoconstituents which have many benefits in different aspects. The essential oil contains phytochemicals such as phenol, phenolic ester, and coumarin, whereas many compounds can been identified in the whole part such as terpenoid, steroid, chromene, pyrrolizidine alkaloid, and flavonoid. Empirically, this herb has been used as an antihemorrhagic, antiseptic, antileprosy, and wound-healing agent. This article reviews the potency of the herb in medication according to the chemical substances being deposited, which are collected from numerous studies, followed by its in silico bioactivity prediction as well as its pharmaceutical dosage form formulation.
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MICROCHEMICAL JOURNAL
·
2011
·
IF 2.48
Yunchang Fan,Sheli Zhang,Jichuan Kong,Juan Miao
In this work, the interaction of an ionic liquid (IL) 1-octyl-3-methylimidazolium chloride ([Omim]Cl) and L -tryptophan was investigated with fluorescence spectroscopic method. The quenching mechanism and binding characteristics of [Omim]Cl with L -tryptophan at different temperatures were studied from fluorescence measurement. The thermodynamic parameters such as free energy change (Δ G ), enthalpy change (Δ H ) and entropy change (Δ S ), were calculated by thermodynamic equations. The experimental results indicated that [Omim]Cl and L -tryptophan had weak interaction and the mechanism of quenching belonged to static quenching. Hydrogen bonds and van der Waals interactions played important roles in the binding of [Omim]Cl with L -tryptophan.
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Membranes
·
2026
·Vol. 16
·期 10
·344
·
IF 4.2
Renxi Zhu,Qiang Wang,Zhiwei Chen,Feng Chen,Congcong Yang,Jianping Tao,Lunjie Zhao,Yukui Gao,Jingwei Zhang,Dan Li
Background: Diabetic skin wounds arise from chronic hyperglycaemia, peripheral neuropathy, microvascular dysfunction, and compromised immunity. Chronic ulcers are further complicated by infection and local ischaemia–hypoxia, which collectively impede tissue repair. Conventional interventions, including glycaemic control, anti-infective therapy, debridement, and wound dressings, can only partially relieve clinical symptoms. Since these strategies fail to reverse ischaemia–hypoxia or restore the reparative capacity of endogenous cells, ulcers often recur, healing outcomes remain unsatisfactory, and limb amputation may become unavoidable. To overcome these drawbacks, we fabricated a bilayer electrospun microfibre membrane for diabetic wound repair.Methods: An electrospun microfibre membrane with a bilayer structure was fabricated. The outer layer, composed of polycaprolactone (PCL), provided mechanical support and enabled sustained release of mitomycin C (MMC). The inner layer, formulated with a 7:3 ratio of poly-L-lactic acid (PLLA) to gelatine (Gel), was designed to match the mechanical properties of the wound bed and was loaded with dimethyloxalylglycine (DMOG). Antibacterial assays againstEscherichia coliand Staphylococcus aureus, as well as proangiogenic evaluations in ischaemic tissues, were conducted to characterise this composite membrane.Results: The outer PCL layer provided mechanical support and enabled sustained MMC release, producing effective antibacterial effects againstE. coliandS. aureus. The DMOG-incorporated inner PLLA/GEL layer displayed favourable mechanical compatibility with wound tissue and successfully promoted angiogenesis under ischaemic conditions. By remodelling the wound microenvironment, the biomaterial improved vascularisation and decreased the bacterial load, ultimately accelerating the repair of diabetic skin wounds.Conclusions: This bilayer electrospun membrane integrates antibacterial and pro-angiogenic functions, showing promising application potential for the treatment of diabetic skin wounds.
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