AAPS PHARMSCITECH
·
2022
·Vol. 23
·期 6
·1-11
·
IF 4.026
Zou Linghui,Zhang Zhongbin,Chen Jinqing,Yang Xu,Li Yuyang,Tang Jing,Du Xiaolu,Tang Ling,Liang Dan,Zhu Xiaoyong,Feng Jianfang,Ding Wenya
β-Cyclodextrin (CD) and chitosan (CS) have attracted great attention due to their unique properties and structures. β-Cyclodextrin-grafted chitosan (CD-CS) has been widely used as a drug carrier to prepare nano-formulations for drug delivery. However, few researches have been conducted to investigate the effect of CD-CS as an excipient on cellular uptake and intestinal absorption. Herein, Caco-2 cells were used to investigate the influence of CD-CS on cellular uptake. The MTT assay showed that CD-CS was non-toxic to Caco-2 cells in concentrations ranging from 15.62 to 125 μg/mL. Confocal laser microscopy and flow cytometry measurements indicated that the uptake ability of Caco-2 cells was significantly enhanced after being treated with CD-CS at a concentration of 31.25 μg/mL or incubation for 0.5 h, and the uptake enhancement gradually increased with increasing CD-CS concentration and incubation time. The Caco-2 monolayer cell model and the everted intestinal sac method were employed to preliminarily explore the mechanism of the improved intestinal absorption. The results demonstrated that CD-CS might open the tight junctions and enhance the clathrin-dependent endocytosis, macro-pinocytosis, and phagocytosis of the intestinal epithelial cells. Such findings can serve as references and inspiration for the design of absorption enhancers. Graphical abstract
✓ 已验证
产品关联
DOI
PubMed
高影响因子
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
·
2022
·Vol. 194
·期 12
·6021-6036
·
IF 3.094
Zhang Xin,Wang Yibing,Liu Jiaqi,Wang Weiyang,Yan Xuecui,Zhou Yifa,Cui Jing,Yuan Ye
Because β-1,6-galactans are significant components in arabinogalactans from plant cell walls, identifying selective endo-β-1,6-galactanases is crucial to degrading these polysaccharides and to analyzing and modifying their structures. Here, we cloned and expressed in E. coli a novel endo-β-1,6-galactanase in the glycosidic hydrolase family 30 (GH30) from Penicillium oxalicum . Our recombinant PoGal30 hydrolase (1464 bp gene) that contains an N-terminal His-tag for purification by nickel affinity chromatography has a specific activity of 3.8 U/mg on the substrate de-arabinosylated gum Arabic (dGA) polysaccharide. The enzyme has 487 residues with a molecular mass of 60 kDa, an isoelectric point of 6, and functional pH and temperature optima of pH 2.5 to pH 5.0 and 40 °C, respectively. While the activity of PoGal30 is activated by Mg 2+ (5 or 50 mmol/L), it is completely inhibited by Cu 2+ and Fe 3+ (50 mmol/L) and partially inhibited by Hg 2+ , EDTA, and SDS (50 mmol/L). The enzyme demonstrates high specificity towards β-1,6-galactosidic linkages in dGA, but is inactive against aryl-glycosides and galactobioses with different linkages. Using PoGal30 is, therefore, an effective approach to analyzing the fine structure of polysaccharides and preparing bioactive oligosaccharides.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LUMINESCENCE
·
2022
·Vol. 37
·期 8
·1290-1299
·
IF 2.613
Dan Li,Peipei Zhou,Yufei Hu,Gongke Li,Ling Xia
In recent years, fluorescent organic–inorganic hybrid nanomaterials have received much interest as potential fluorescent sensor materials. In this study, fluorescent organic–inorganic hybrid nanomaterials (POSS@ANT) were created using polyhedral oligomeric silsesquioxane as the precursor and 9,10-bromoanthracene as the monomer. The morphology and composition of POSS@ANT, as well as its pore characteristics and fluorescence properties were studied. POSS@ANT displayed steady fluorescence emission at an excitation wavelength of 374 nm. Next, a β-carotene fluorescence sensor was developed using the capacity of β-carotene to quench the fluorescence of POSS@ANT. The quenching process is linked to acceptor electron transfer and energy transfer, and the sensor has a high selectivity for β-carotene. This β-carotene fluorescence analysis method that we established has a linear range of 0.2–4.3 mg/L and a detection limit of 0.081 mg/L. Finally, it was used to quantify β-carotene in health products, the recovery rate was 91.1–109.9%, the relative standard deviation (RSD) was 2.2–4.3%, and the results were comparable with the results of high-performance liquid chromatography. The approach is reliable and can be used to determine β-carotene in health products.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2022
·Vol. 14
·期 17
·1739-1746
·
IF 3.532
Zixuan Chen,Yinan Li,Haixin Qin,Xiaoyan Yang,Wei Cao
β-Amyloid (Aβ) peptides can bind both Cu2+ and heme cofactors simultaneously to form heme–Cu2+–Aβ complexes, which are proposed to generate toxic partially reduced oxygen species (PROS, e.g., H2O2) and play a vital role in Alzheimer's disease (AD). In this paper, a competitive dual-mechanism-driven electrochemiluminescence (ECL) aptasensor integrating the synergistic enhancement and steric hindrance effect was described for Aβ detection. Specifically, graphite carbon nitride (g-C3N4) as an effective ECL luminescent substrate and Au nanoparticles were sequentially assembled on the Au electrode surface, and then a thiol-modified aptamer for capturing Aβ peptide was attached to the surface of the electrode through the Au–S bond. Aβ peptides were simultaneously incubated with heme and Cu2+, and the forming heme–Cu2+–Aβ complexes were subsequently anchored on the electrode through the specific recognition between the target Aβ and the aptamer. When the concentration of the target Aβ is low, the synergistic enhancement effect arising from K2S2O8 with in situ generated H2O2 is predominant, resulting in an increase in the ECL signal of g-C3N4. In contrast, when the concentration of Aβ is high, the steric hindrance effect generated from heme–Cu2+–Aβ complexes is dominant, leading to a decrease in the ECL signal. The present sensor exhibits a favorable linear response for the detection of Aβ with a relatively low detection limit of 0.24 pM, and provides a more sensitive and selective platform for bioanalysis.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL PHYSICS LETTERS
·
2022
·
IF 2.719
Xinhe Liu,Yong Kang,Yi Wang
In this work, novel visible-light responsive β-Bi 2 O 3 /Ag 2 WO 4 p-n heterojunction photocatalysts were prepared by a simple in situ deposition method and characterized using XRD, FT-IR, SEM, TEM, XPS, UV–vis and PL. The uniform deposition of Ag 2 WO 4 particles on β-Bi 2 O 3 flakes to form heterojunctions, exhibited excellent photocatalytic activity for the degradation of methylene blue (MB) under visible light in 30 min. Compared with pure β-Bi 2 O 3 and Ag 2 WO 4 samples, the kinetic reaction rate constant of MB degradation by β-Bi 2 O 3 /Ag 2 WO 4 was 48.47 times higher than that of β-Bi 2 O 3 , and the degradation rate was as high as 98.8% with good cycling stability.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2022
·
IF 14.224
Long Kong,Ruixin Yan,Min Liu,Junqing Xu,Takeshi Hagio,Ryoichi Ichino,Liang Li,Xinde Cao
In this study, β-Cyclodextrin (CD) modified Fe 3 S 4 nanomaterials were synthesized by a one-step facile strategy and investigated for the removal of Cr(VI). The resulted CD-Fe 3 S 4 exhibited enhanced removal efficiency toward Cr(VI) than bared Fe 3 S 4 with a maximum capacity of 220.26 mg·g -1 as the molar ratio of CD-to-Fe 3 S 4 at 0.2. The effective performance of CD-Fe 3 S 4 toward Cr(VI) could well maintain under oxic conditions and a wide pH range of aqueous solution. A high selectivity for Cr(VI) was achieved in the presence of coexisting cations and anions. More significantly, a single treatment step of CD-Fe 3 S 4 effectively removed chromium from actual electroplating wastewater to the detection limit of 0.004 mg·L -1 that far below the WHO limitation of Cr (VI) (<0.05 mg·L -1 ) combing with the rapid magnetic separation without adjusting the pH value of wastewater at 7. The effective removal of Cr (VI) by CD-Fe 3 S 4 involved a complex process of surface adsorption/reduction, and solution homogenous reduction and subsequent sequestration of Cr(III) achieving the effective removal of aqueous total Cr. The superior Cr (VI) removal capability and facial separation of CD-Fe 3 S 4 attained its prominent potential application as an effective material for the Cr(VI) removal.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CRYSTAL RESEARCH AND TECHNOLOGY
·
2022
·Vol. 57
·期 4
·2100162
·
IF 1.599
Yanli Ding,Zhaohan Li
The low luminous efficiency of upconversion materials limits their practical applications in various fields. The present study focuses on the efficient upconversion enhancement of β-NaYF 4 :Yb,Er induced by Bi 3+ doping. The XRD and SEM results show that introducing Bi 3+ into the matrix lattice changes the microstructure and particle size of the crystal. The photoluminescence spectra show that at 6% Bi 3+ doping, the red and green emission intensities are enhanced by 12 and 8 times, respectively. The reason could be the lattice distortion caused by Bi 3+ doping, breaking the partial forbidden transition of rare-earth ions. The fluorescence lifetime measurement reveals that the Bi 3+ doping changes the environment around erbium ions, affecting the energy level lifetime of erbium ions, explaining the luminescence dynamics law. The present study provides a facile route to achieve nano/microstructures with intense upconversion luminescence with potential applications in optoelectronic devices.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF CHEMICAL PHYSICS
·
2023
·Vol. 158
·期 20
·
IF 4.4
Zhang Qin,Zhou Zhongneng,Deng Bodan,Wang Bingyao,Kang Xiu-Wen,Chen Jinquan,Ding Bei,Zhong Dongping
Phototriggers are useful molecular tools to initiate reactions in enzymes by light for the purpose of photoenzymatic design and mechanistic investigations. Here, we incorporated the non-natural amino acid 5-cyanotryptophan (W 5CN ) in a polypeptide scaffold and resolved the photochemical reaction of the W 5CN –W motif using femtosecond transient UV/Vis and mid-IR spectroscopy. We identified a marker band of ∼2037 cm −1 from the CN stretch of the electron transfer intermediate W 5CN ·− in the transient IR measurement and found UV/Vis spectroscopic evidence for the W ·+ radical at 580 nm. Through kinetic analysis, we characterized that the charge separation between the excited W 5CN and W occurs in 253 ps, with a charge-recombination lifetime of 862 ps. Our study highlights the potential use of the W 5CN –W pair as an ultrafast phototrigger to initiate reactions in enzymes that are not light-sensitive, making downstream reactions accessible to femtosecond spectroscopic detection.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nano Research
·
2021
·Vol. 15
·期 4
·3026-3033
·
IF 8.897
Sun Hao,Yin Hua-Qing,Shi Wenxiong,Yang Lu-Lu,Guo Xiang-Wei,Lin Hong,Zhang Jiangwei,Lu Tong-Bu,Zhang Zhi-Ming
Electrochemical nitrogen reduction reaction (NRR) under ambient conditions is highly desirable to achieve sustainable ammonia (NH 3 ) production via an alternative carbon free strategy. Single-atom catalysts (SACs) with super high atomic utilization and catalytic efficiency exhibit great potential for NRR. Herein, a high-performance NRR SAC is facilely prepared via a simple deposition method to anchor Au single atoms onto porous β-FeOOH nanotubes. The resulting Au-SA/FeOOH can efficiently drive NRR under ambient conditions, and the NH 3 yield reaches as high as 2,860 µg·h −1 ·mg Au −1 at −0.4 V vs. reversible hydrogen electrode (RHE) with 14.2% faradaic efficiency, much superior to those of all the reported Au-based electrocatalysts. Systematic investigations demonstrate that the synergy of much enhanced N 2 adsorption, directional electron export, and mass transfer ability in Au-SA/FeOOH greatly contributes to the superior NRR activity. This work highlights a new insight into the design of high efficient NRR electrocatalysts by combination of porous metal oxide matrix and highly active single-atom sites.
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2021
·
IF 9.229
Wenjuan Wang,Miaomiao Liu,Weiqun Gao,Yan Sun,Xiaoyan Dong
β-Amyloid (Aβ) fibrillogenesis is closely associated with the pathogenesis of Alzheimer’s disease (AD), so detection and inhibition of Aβ aggregation are of significance for the theranostics of AD. In this work, the coassembled nanoparticles of chitosan and hyaluronic acid cross-linked with glutaraldehyde (CHG NPs) were found to work as a theranostic agent for imaging/probing and inhibition of Aβ fibrillization both in vitro and in vivo. The biomass-based CHG NPs of high stability exhibited a wide range of excitation/emission wavelengths and showed binding affinity toward Aβ aggregates, especially for soluble Aβ oligomers. CHG NPs displayed weak emission in the monodispersed state, while they remarkably emitted increased red fluorescence upon interacting with Aβ oligomers and fibrils, showing high sensitivity with a detection limit of 0.1 nM. By comparing the different fluorescence responses of CHG NPs and Thioflavin T to Aβ aggregation, the Aβ oligomerization rate during nucleation can be determined. Moreover, the fluorescence recognition behavior of CHG NPs was selective. CHG NPs specifically bind to negatively charged amyloid aggregates but not to positively charged amyloids and negatively charged soluble proteins. Such enhancement in fluorescence emission is attributed to the clustering-triggered emission effect of CHG NPs after interaction with Aβ aggregates via various electronic conjugations and hydrogen bonding, electrostatic, and hydrophobic interactions. Besides fluorescent imaging/probing, CHG NPs over 360 μg/mL could almost completely inhibit the formation of Aβ fibrils, exhibiting the capability of regulating Aβ aggregation. In-vivo assays with Caenorhabditis elegans CL2006 demonstrated the potency of CHG NPs as an effective theranostic nanoagent for imaging Aβ plaques and inhibiting Aβ deposition. The findings proved the potential of CHG NPs for development as a potent agent for the diagnosis and treatment of AD.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Yingting Luo,Yaqi Tu,Fazheng Ren,Hao Zhang
The Maillard reaction products (MRPs) between polydextrose (PDX), a popular polysaccharide in formula powder, and β-lactoglobulin (β-LG), a major whey protein, were studied in aggregation degree, structure, hydrophobic, antigenic and antioxidant activity changes of β-LG. Incubation of PDX and β-LG (60 ℃, 79% relative humidity) for up to 72 h yielded MRPs with increases in furosine, UV absorbance, fluorescence intensity and loss of free amino groups. High molecular weight β-LG-PDX MRPs were observed by SDS-PAGE. Circular dichroism spectroscopy revealed negligible change in β-LG secondary structure. Changes in the tertiary structure of β-LG were detected by tryptophan fluorescence spectroscopy consistent with an increase in surface hydrophobicity of heated β-LG-PDX. Antigenicity reduction of β-LG in β-LG-PDX reached its peak when heated for 24 h. After heating for 72 h, DPPH radical-scavenging activity of β-LG-PDX increased by 7.4-fold, and the ferric reducing antioxidant power reached 61.1 µmol ascorbic acid/g protein.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Chemical Biology & Drug Design
·
2021
·Vol. 99
·期 2
·362-372
·
IF 2.817
Jia-Zhu Chigan,Jia-Qi Li,Huan-Huan Ding,Yin-Sui Xu,Lu Liu,Cheng Chen,Ke-Wu Yang
Bacterial resistance caused by metallo-β-lactamases (MβLs) has become an emerging public health threat, and the development of MβLs inhibitor is an effective way to overcome the resistance. In this study, thirteen novel O -aryloxycarbonyl hydroxamates were constructed and assayed against MβLs. The obtained molecules specifically inhibited imipenemase-1 (IMP-1) and New Delhi metallo-β-lactamase-1, exhibiting an IC 50 value in the range of 0.10–18.42 and 0.23–22.33 μM, respectively. The hydroxamate 5 was found to be the most potent inhibitor, with an IC 50 of 0.1 and 0.23 μM using meropenem and cefazolin as substrates. ICP-MS analysis showed that 5 did not coordinate to the Zn(II) ions at the active site of IMP-1, while the rapid dilution, thermal shift and MALDI-TOF assays revealed that the hydroxamate formed a covalent bond with the enzyme. Cytotoxicity assays indicated that the hydroxamates have low toxicity in MCF-7 cells. This work provided a potent scaffold for the development of MβLs inhibitors.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）