LABORATORY INVESTIGATION
·
2023
·
IF 5
Zhiguang Chen,Haibin Zhao,Lingshuai Meng,Shengwei Yu,Zhenning Liu,Jinqi Xue
Fracture healing is a complex physiological process in which angiogenesis plays an essential role. Microfibril-associated glycoprotein-2 (MAGP2) has been reported to possess a proangiogenic activity via integrin αvβ3, yet its role in bone repair is unexplored. In this study, a critical-sized femoral defect (2 mm) was created in mice, followed by the delivery of an adenovirus-based MAGP2 overexpression vector or its negative control at the fracture site. At days 7, 14, 21, and 28 postfracture, bone fracture healing was evaluated by radiography, micro–computed tomography, and histopathologic analysis. Adenovirus-based MAGP2 overexpression vector–treated mice exhibited increased bone mineral density and bone volume fraction. MAGP2 overexpression contributed to an advanced stage of endochondral ossification and induced cartilage callus into the bony callus. Further analysis indicated that MAGP2 was associated with enhanced angiogenesis, as evidenced by marked MAGP2 and integrin αvβ3 costaining and increased levels of endothelial cell markers such as endomucin and CD31, as well as elevated phosphorylation of protein tyrosine kinase 2 (PTK2) and AKT serine/threonine kinase 1 (AKT) in the callus. In vitro , recombinant human MAGP2 treatment enhanced the viability, migration, and tube formation ability of human microvascular endothelial cells, which was partially reversed by integrin αvβ3 inhibition or MK-2206, a specific AKT inhibitor. Inhibition of integrin αvβ3 abolished MAGP2-induced PTK2 and AKT activation. Taken together, our data provide the first evidence that MAGP2 promotes angiogenesis and bone formation by activating the integrin αvβ3/PTK2/AKT signaling pathway.
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DOI
PubMed
高影响因子
POLYMER TESTING
·
2025
·
IF 6
Ke Li,Lu-Ming Zhou,Yun-Xiao Zhang,Yuan-Xiang Zhou
Electrical treeing is a primary degradation pathway in cross-linked polyethylene (XLPE) cable insulation, making enhancement of the tree-initiation voltage (TIV) critical for reliable high-voltage operation. We report a peroxide-assisted grafting strategy that covalently incorporates 2-vinylanthraquinone (VAQ) into XLPE during standard crosslinking, thereby addressing the migration and durability limitations of physically blended stabilizers. The grafted VAQ introduces deep electronic traps and mitigates defect-driven degradation, increasing the characteristic TIV by more than 160 % and markedly suppressing tree growth under AC voltage. This migration-resistant, trap-engineered stabilization provides a practical route to improve long-term insulation reliability and is directly relevant to the design of ultra-high-voltage polymeric cables.
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DOI
高影响因子
FUEL
·
2025
·
IF 7.5
Wanpeng Li,Xuewei Zhang,Guangmai Zhao,Tianhan Zhu,Mi Luo,Tianyu Gao,Chenguang Wang,Xiaoyu Chen,Jin Tan,Yuhe Liao,Haiyong Wang
The efficient and sustainable transformation of biomass-derived furfural into value-added chemicals is a key challenge in green chemistry. In this study, a series of Pt/CeO 2 catalysts with varying Pt loadings (0.1–2.0 wt%) were synthesized via a carbonization–reduction method and applied for the selective conversion of furfural to 2-furfural diethanol in ethanol under mild conditions. Comprehensive characterization using XRD, XPS, EPR, TEM, and Py-FTIR revealed that the 1.0 wt% Pt/CeO 2 catalyst exhibited optimal properties, including high dispersion of metallic Pt⁰, abundant oxygen vacancies, enriched Ce 3+ species, and dominant Lewis acid sites. Catalytic tests demonstrated that under mild conditions (20 °C, 4 MPa N 2 ), the 1.0 wt% Pt/CeO 2 catalyst achieved the highest yield (92.17 %) and furfural conversion (95.41 %). Mechanistic studies suggest a proposed redox-driven tandem hydrogenation–condensation pathway, where the synergistic effect between Pt⁰ sites and oxygen vacancies facilitates furfural activation, hydrogen transfer, and product formation. This work provides a mechanistic understanding of furfural upgrading over Pt/metal oxide interfaces and offers a promising strategy for the rational design of high-performance catalysts for biomass valorization.
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高影响因子
JOURNAL OF FOOD SCIENCE
·
2025
·Vol. 91
·期 1
·e70646
·
IF 3.4
Qi Fang,Xinyao Liu,Wei Huang,Kai Qian,Yanjianxiong Yang,Jing Li,Zeyuan Deng
Docosahexaenoic acid (DHA), one of the most critical polyunsaturated fatty acids, is vital for the neurological growth and cognitive function of infants and children. Approximately 98% of DHA in breast milk exists as triglycerides, with 60% esterified at the sn-2 position. To demonstrate the necessity of mimicking the form of DHA present in breast milk in nutritional food for young children, this study administered diets with varying sn-2 DHA contents (10%, 30%, and 50%) to four groups of mice and analyzed their behavioral performance, brain DHA concentration, expression of brain fatty acid transport proteins, histopathology, and expression of synaptic-related proteins in the hippocampus after 4 weeks. The results showed that compared with the control group, mice in the 50% sn-2 DHA group exhibited superior learning and memory capabilities in behavioral tests, with the most pronounced behavioral improvements in mice, which correlated with higher brain DHA accumulation (from 0.870 ± 0.055 mg/g brain to 1.809 ± 0.132 mg/g brain, p < 0.05), increased levels of MFSD2A (1.40-fold, p > 0.05), FABP5 (2.36-fold, p < 0.05), FATP1 (1.47-fold, p < 0.05), and ACSL6 (1.48-fold, p < 0.05), improved hippocampal neuron morphology, and enhanced the level of BDNF (1.55-fold, p < 0.05), SYN (1.45-fold, p < 0.05), and PSD-95 (1.57-fold, p < 0.05). These findings establish a foundation for developing DHA nutritional supplements.
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF ENDOCRINOLOGY
·
2018
·Vol. 238
·期 2
·137-149
·
IF 4.012
Lihong Fu,Yixuan Qiu,Linyan Shen,Canqi Cui,Shuang Wang,Shujie Wang,Yun Xie,Xinjie Zhao,Xianfu Gao,Guang Ning,Aifang Nie,Yanyun Gu
An increasing amount of evidence suggests that the delayed effect of antibiotics (abx) on gut microbiota after its cessation is not as favorable as its immediate effect on host metabolism. However, it is not known how the diverse abx-dependent metabolic effects influence diabetic subjects and how gut microbiota is involved. Here, we treated db/db mice with abx cocktail for 12 days and discontinued for 24 days. We found that db/db mice showed decreased body weight and blood glucose after abx treatment, which rapidly caught up after abx cessation. Twenty-four days after abx withdrawal, db/db mice exhibit increased plasma, hepatic total cholesterol (TC) levels and liver weight. The gut microbiota composition at that time showed decreased relative abundances (RAs) of Desulfovibrionaceae and Rikenellaceae, increased RA of Erysipelotrichaceae and Mogibacteriaceae, which were correlating with the reduced short-chain fatty acids (SCFAs) in gut content, such as propionic acid and valeric acid and with the elevated fecal taurine-conjugated bile acids (BAs) levels. The molecular biology studies showed inhibited hepatic BA synthesis from cholesterol, impeded intracellular transportation and biliary excretion of cholesterol that all conferred to liver TC accumulation. The associations among alterations of gut microbiota composition, microbial metabolite profiles and host phenotypes suggested the existence of gut microbiota-linked mechanisms that mediate the unfavorable delayed effects of abx on db/db mice cholesterol metabolism. Thus, we call upon the caution of applying abx in diabetic animal models for studying microbiota-host interaction and in type 2 diabetes subjects for preventing chronic cardiovascular consequences. An increasing amount of evidence suggests that the delayed effect of antibiotics (abx) on gut microbiota after its cessation is not as favorable as its immediate effect on host metabolism. However, it is not known how the diverse abx-dependent metabolic effects influence diabetic subjects and how gut microbiota is involved. Here, we treated db/db mice with abx cocktail for 12 days and discontinued for 24 days. We found that db/db mice showed decreased body weight and blood glucose after abx treatment, which rapidly caught up after abx cessation. Twenty-four days after abx withdrawal, db/db mice exhibit increased plasma, hepatic total cholesterol (TC) levels and liver weight. The gut microbiota composition at that time showed decreased relative abundances (RAs) of Desulfovibrionaceae and Rikenellaceae, increased RA of Erysipelotrichaceae and Mogibacteriaceae, which were correlating with the reduced short-chain fatty acids (SCFAs) in gut content, such as propionic acid and valeric acid and with the elevated fecal taurine-conjugated bile acids (BAs) levels. The molecular biology studies showed inhibited hepatic BA synthesis from cholesterol, impeded intracellular transportation and biliary excretion of cholesterol that all conferred to liver TC accumulation. The associations among alterations of gut microbiota composition, microbial metabolite profiles and host phenotypes suggested the existence of gut microbiota-linked mechanisms that mediate the unfavorable delayed effects of abx on db/db mice cholesterol metabolism. Thus, we call upon the caution of applying abx in diabetic animal models for studying microbiota-host interaction and in type 2 diabetes subjects for preventing chronic cardiovascular consequences.
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DOI
PubMed
高影响因子
RARE METALS
·
2025
·
IF 11
Zhen-Xuan Ma,Jia-Guang Zheng,Mei-Ling Lv,Cong Li,Chang-Hai Wu,Pan-Pan Zhou,Chao Su,Lei Ge
en This research uncovered the influence of nitrogen-doped MXene Ti 3 CN on the reversible hydrogen storage properties of Mg(BH 4 ) 2 . The Mg(BH 4 ) 2 -20Ti 3 CN composite began releasing hydrogen at a temperature of 80°C. In addition, the composite discharged over 8.8 wt% hydrogen at a comparatively lower temperature of 270°C, whereas the pure Mg(BH 4 ) 2 emitted merely 5.9 wt% hydrogen under identical circumstances, which exhibited remarkably enhanced dehydrogenation kinetics. Furthermore, upon completion of four cycles, Mg(BH 4 ) 2 -20Ti 3 CN retained a reversible hydrogen storage capacity of 4.5 wt%, representing an 80% improvement over the undoped Mg(BH 4 ) 2 . In the course of the dehydrogenation phase, the boron atoms in Mg(BH 4 ) 2 were anchored by nitrogen atoms in Ti 3 CN to form B-N bonds, which helped suppress the formation of MgB 12 H 12 . During the subsequent rehydrogenation process, the B-N bonds broke, and the boron atoms reparticipated in the reversible transformation into [BH 4 ] − clusters. Meanwhile, the formation of Ti 0 by the reaction of Mg(BH 4 ) 2 with Ti 3 CN weakened the B-H bond energy, and the layered structure provided an effective way for hydrogen spillover. These factors collectively improved the reversible hydrogen storage capabilities of Mg(BH 4 ) 2 . 摘要 zh 本研究揭示了氮掺杂MXene Ti 3 CN对Mg(BH 4 ) 2 可逆储氢性能的影响。Mg(BH 4 ) 2 -20Ti 3 CN复合材料在温度80°C时开始放氢。此外,在270°C的较低温度下,该复合材料的氢排放量超过8.8 wt%,而在相同的条件下,纯Mg(BH 4 ) 2 的氢排放量仅为5.9 wt%,脱氢动力学显著增强。在完成四个循环后,Mg(BH 4 ) 2 -20Ti 3 CN保留了4.5 wt%的可逆储氢容量,比未掺杂的Mg(BH 4 ) 2 提高了80%。在脱氢过程中,Mg(BH 4 ) 2 中的硼原子被Ti 3 CN中的氮原子锚定形成B-N键,有助于抑制MgB 12 H 12 的形成。在随后的再氢过程中,B-N键断裂,硼原子重新参与可逆转化为[BH 4 ] − 簇。同时,Mg(BH 4 ) 2 与Ti 3 CN反应生成Ti 0 ,削弱了B-H键能,层状结构也为氢溢出提供了有效路径。这些因素共同提高了Mg(BH 4 ) 2 的可逆储氢能力。
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高影响因子
BIOMEDICINE & PHARMACOTHERAPY
·
2016
·
IF 2.326
ZhenQiu Chen,QiuShi Wei,GuoJu Hong,Da Chen,Jiang Liang,Wei He,Mei Hui Chen
Bone marrow stromal cells (BMSCs) have proven to be useful for the treatment of numerous human diseases . However, the reparative ability of BMSCs is limited by their poor migration. Polydatin , widely used in traditional Chinese remedies, has proven to exert protective effects to BMSCs. However, little is known about its role in BMSCs migration. In this study, we studied the effects of polydatin on rat BMSCs migration using the scratch wound healing and transwell migration assays. Our results showed polydatin could promote BMSCs migration. Further experiments showed activation of ERK 1/2, but not JNK , was required for polydatin-induced BMSCs migration, suggesting that polydatin may promote BMSCs migration via the ERK 1/2 signaling pathways . Taken together, our results indicate that polydatin might be beneficial for stem cell replacement therapy by improving BMSCs migration.
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2020
·
IF 5.162
Jie Chen,Xicheng Wang,Hua Ye,Zhenyan Yu,Longbao Feng,Jing Zhou,Yajin Che
As one of the most commonly used materials in liver tissue engineering, hydrogel has received much attention in recent years. In this work, we prepared a gelatin methacrylate (GelMA)/oxidized hyaluronic acid (OHA)/galactosylated chitosan (Gal-CS)/Fe (III)@ [email protected] 200 (TA200) hydrogel loaded with insulin-like growth factor 2 (IGF-2) for regeneration of damaged hepatocytes. Fe (III)@TA microspheres served as carrier to achieve sustained release of IGF-2 to promote hepatocytes regeneration. Galactose ligands could bind to the asialoglycoprotein receptor (ASGPR) on the surface of hepatocytes. Galactosylated chitosan could significantly increase the specific function of hepatocytes. The hydrogel we prepared had a storage modulus of 1100 Pa and was suitable for migration of hepatocytes. The release ratio of IGF-2 could reach up to 90% within 14 days. For carbon tetrachloride (CCl 4 ) induced human hepatic stellate cell line LX2 damage, GelMA/OHA/Gal-CS/TA200 hydrogel could significantly improve the survival of LX2 cells. The expression of HNF-4α and transferrin was detected in LX2 cells treated with hydrogel, indicating that the specific function of the liver was also restored. In summary, the GelMA/OHA/Gal-CS/TA200 hydrogels could be used as new tissue engineering scaffolds for the construction of artificial livers.
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DOI
PubMed
高影响因子
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY
·
2018
·Vol. 95
·期 1
·79-88
·
IF 1.601
Ting Zheng,Zhenyu Wu,Qinglong Xie,Meizhen Lu,Fan Xia,Guangquan Wang,Yong Nie,Jianbing Ji
Owing to the decrease of global oil price, development of downstream value-added products is important to biodiesel industry. In this study, we used palmitic acid methyl ester (PAME) as a starting material to produce 2-ethylhexyl palmitate (2-EHP), an environmentally friendly biolubricant product, which was derived from the transesterification of fatty acid methyl esters and long chain fatty alcohols. Conventional synthetic routes of 2-EHP have disadvantages, including high catalyst price, low conversion efficiency, and pollution issues. To solve these problems, in situ transesterification of PAME with 2-ethylhexanol (2-EH) was conducted over unsupported potassium carbonate as heterogeneous catalyst. The optimal reaction temperature, 2-EH to PAME molar ratio, and catalyst to PAME mass ratio were 180 °C, 3:1, and 3.0 wt%, respectively. The PAME conversion reached up to 100% within 1 hour under the optimal conditions. In addition, a kinetic model describing the experimental data over a temperature range of 160–180 °C was developed. The dependence of kinetic rate constant ( k ) on temperature was evaluated, and the activation energy ( E a ) for the transesterification of PAME with 2-EH was calculated to be 57.04 kJ mol −1 .
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高影响因子
JOURNAL OF LOSS PREVENTION IN THE PROCESS INDUSTRIES
·
2024
·
IF 3.6
Chenyang Pu,Zhi Wang,Boren Zheng,Bin Zhang
1,2,4-Triazole is a common intermediate used in the synthesis of pesticides and medicine, and has a very wide range of applications. As a kind of polynitrogen heterocycles, 1,2,4-triazole will release a lot of heat during thermal decomposition. If there is no comprehensive understanding of its pyrolysis behavior, there will be a very large thermal safety risk in the process involving 1,2,4-triazole in industrial production. At the same time, the instability of the substance may be affected by the presence of impurities, and the existing research on this aspect is quite scarce. Therefore, it is very necessary to study the effect of adding other substances on the thermal decomposition of 1,2, 4-triazole. The thermal decomposition characteristics and thermal runaway behavior of 1,2, 4-triazoles were revealed by thermodynamic experiments and various kinetic theoretical methods. According to the risk matrix, the thermal runaway danger level of 1,2, 4-triazole was determined to be level Ⅲ, which was an unacceptable risk. The results show that 1,2, 4-triazole has a huge heat release and the effect of thermal runaway is serious. In this study, three substances involved in the industrial production of fluconazole and flutriafol mixed with 1,2, 4-triazole were selected to explore their influence on the thermal decomposition of 1,2, 4-triazole under non-working conditions. DSC and ARC experiments consistently showed that these three impurities could reduce the reaction heat release of 1,2, 4-triazole to varying degrees, and confirmed that these three impurities could reduce the risk of thermal runaway of 1,2, 4-triazole to varying degrees. Among them, sodium hydride has the most outstanding performance, both in terms of reducing reaction heat release and reducing adiabatic temperature rise. The results can provide reference for loss control of 1,2,4-triazole in industrial applications.
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高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2020
·
IF 3.232
Xiao Wang,Mei-Hong Liu,Qing Xu,De-Long Zhang,Pei Zhang,Wing-Han Wong
Er 3+ -doped NaLa(WO 4 ) 2 is a promising phosphor material for applications in many fields including the ratiometric thermometry based on thermal effect of fluorescence intensity ratio (FIR) of green fluorescence of Er 3+ , which is directly correlated with Judd-Ofelt parameters Ω i (i = 2, 4, 6). Present paper reports synthesis and Judd-Ofelt spectroscopic properties of Er 3+ -doped NaLa(WO 4 ) 2 µm-sized phosphor. The phosphor was synthesized by solid-state chemical reaction and characterized by X-ray diffraction and Raman scattering techniques. The results show that the phosphor is dominated by NaLa(WO 4 ) 2 crystalline phase and has a maximum phonon energy 927 cm −1 . Judd-Ofelt analysis was done for the phosphor using a safe, reliable method based on diffuse reflection spectrum and Er 3+ 1.5 μm fluorescence lifetime . First, diffuse reflection spectrum of the phosphor was measured and relative absorption spectrum was calibrated from it using Kubelka-Munk theory. Second, Er 3+ 1.5 μm fluorescence lifetime of the phosphor was measured and absorption cross-section spectrum was obtained based on the assumption that the 1.5 μm emission has 100% quantum efficiency. Finally, based on the absorption cross-section spectrum, standard Judd-Ofelt analysis was carried out to extract the Ω i . Radiative rate, fluorescent branch ratio and radiative lifetime of some transitions have been obtained from the known Ω i values. In addition, FIR proportional factor was evaluated in terms of Ω i and compared with those values of other materials. The result shows that the phosphor has a better prospect for the application in ratiometric thermometry.
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高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Sizhong Feng,Mengke Xue,Fang Xie,Hongyang Zhao,Yemin Xue
The gene-encoding carboxylesterase (TM1022) from the hyperthermophilic bacterium Thermotoga maritima (T. maritima) was cloned and expressed in Escherichia coli Top10 and BL21 (DE3). Recombinant TM1022 showed the best activity at pH 8.0 and 85 °C and retained 57% activity after 8 h cultivation at 90 °C. TM1022 exhibited good stability at pH 6.0–9.0, maintaining 53% activity after incubation at pH 10.0 and 37 °C for 6 h. The esterase TM1022 exhibited the optimum thermo-alkali stability and kcat/Km (598.57 ± 19.97 s–1mM–1) for pN–C4. TM1022 hydrolyzed poly(ethylene terephthalate) (PET) degradation intermediates, such as bis(2-hydroxyethyl) terephthalate (BHET) and mono(2-hydroxyethyl) terephthalate (MHET). The Km, kcat, and kcat/Km values for BHET were 0.82 ± 0.01 mM, 2.20 ± 0.02 s–1, and 2.67 ± 0.02 mM–1 s–1, respectively; those for MHET were 2.43 ± 0.07 mM, 0.04 ± 0.001 s–1, and 0.02 ± 0.001 mM–1 s–1, respectively. When purified TM1022 was added to the cutinase BhrPETase, hydrolysis of PET from drinking water bottle tops produced pure terephthalic acids (TPA) with 166% higher yield than those obtained after 72 h of incubation with BhrPETase alone as control. The above findings demonstrate that the esterase TM1022 from T. maritima has substantial potential for depolymerizing PET into monomers for reuse.
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高影响因子
危险品化学品经营许可证（带存储）